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Tytuł :
The relationship between melanin production and lipofuscin formation in Tyrosinase gene knockout melanocytes using CRISPR/Cas9 system.
Autorzy :
Kim JH; Department of Applied Chemistry, Dong-Eui University, Busan 614-714, Republic of Korea.
Kim MM; Department of Applied Chemistry, Dong-Eui University, Busan 614-714, Republic of Korea. Electronic address: .
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Źródło :
Life sciences [Life Sci] 2021 Nov 01; Vol. 284, pp. 119915. Date of Electronic Publication: 2021 Aug 25.
Typ publikacji :
Journal Article
MeSH Terms :
Gene Knockout Techniques*
CRISPR-Cas Systems/*genetics
Lipofuscin/*metabolism
Melanins/*biosynthesis
Melanocytes/*enzymology
Monophenol Monooxygenase/*genetics
Animals ; Antioxidants/metabolism ; Base Sequence ; Cell Survival/radiation effects ; Gene Expression Regulation, Neoplastic ; Melanoma, Experimental/genetics ; Melanoma, Experimental/pathology ; Mice ; Ultraviolet Rays
Czasopismo naukowe
Tytuł :
In vivo CRISPR-Cas9 knockout screening using quantitative PCR identifies thymosin beta-4 X-linked that promotes diffuse-type gastric cancer metastasis.
Autorzy :
An HW; Division of Biomedical Convergence, College of Biomedical Science, Kangwon National University, ChunCheon-si, Gangwon-do, South Korea.
Kim SY; Division of Biomedical Convergence, College of Biomedical Science, Kangwon National University, ChunCheon-si, Gangwon-do, South Korea.
Kwon JW; Division of Biomedical Convergence, College of Biomedical Science, Kangwon National University, ChunCheon-si, Gangwon-do, South Korea.
Seok SH; Division of Biomedical Convergence, College of Biomedical Science, Kangwon National University, ChunCheon-si, Gangwon-do, South Korea.
Woo SH; Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, Seoul, South Korea.
Kim DY; Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, Seoul, South Korea.
Park JW; Division of Biomedical Convergence, College of Biomedical Science, Kangwon National University, ChunCheon-si, Gangwon-do, South Korea.
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Źródło :
Molecular carcinogenesis [Mol Carcinog] 2021 Sep; Vol. 60 (9), pp. 597-606. Date of Electronic Publication: 2021 Jun 03.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Biomarkers, Tumor*
CRISPR-Cas Systems*
Gene Knockout Techniques*
Real-Time Polymerase Chain Reaction*
Stomach Neoplasms/*genetics
Stomach Neoplasms/*pathology
Thymosin/*genetics
Animals ; Disease Models, Animal ; Gene Expression ; Humans ; Mice ; Neoplasm Metastasis ; Signal Transduction
Czasopismo naukowe
Tytuł :
Targeted knockout of the gene OsHOL1 removes methyl iodide emissions from rice plants.
Autorzy :
Carlessi M; PlantLab, Institute of Life Sciences, Scuola Superiore Sant'Anna, Pisa, Italy.
Mariotti L; Department of Agriculture, Food and Environment, University of Pisa, Pisa, Italy.
Giaume F; Department of Biosciences, University of Milan, Milan, Italy.
Fornara F; Department of Biosciences, University of Milan, Milan, Italy.
Perata P; PlantLab, Institute of Life Sciences, Scuola Superiore Sant'Anna, Pisa, Italy. .
Gonzali S; PlantLab, Institute of Life Sciences, Scuola Superiore Sant'Anna, Pisa, Italy. .
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Źródło :
Scientific reports [Sci Rep] 2021 Aug 23; Vol. 11 (1), pp. 17010. Date of Electronic Publication: 2021 Aug 23.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Gene Knockout Techniques*
Genes, Plant*
Hydrocarbons, Iodinated/*isolation & purification
Oryza/*genetics
Base Sequence ; CRISPR-Cas Systems/genetics ; Gene Expression Regulation, Plant ; Luciferases/metabolism ; Methyltransferases/genetics ; Methyltransferases/metabolism ; Mutagenesis/genetics ; Plant Leaves/genetics ; Protein Multimerization ; Subcellular Fractions/metabolism
Czasopismo naukowe
Tytuł :
Gene knockout in highly purified mouse hematopoietic stem cells by CRISPR/Cas9 technology.
Autorzy :
Dong Y; State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300020, China.
Bai H; State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300020, China.
Dong F; State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300020, China.
Zhang XB; State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300020, China; Department of Medicine, Loma Linda University, Loma Linda, CA, United States of America.
Ema H; State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300020, China. Electronic address: .
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Źródło :
Journal of immunological methods [J Immunol Methods] 2021 Aug; Vol. 495, pp. 113070. Date of Electronic Publication: 2021 May 04.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
CRISPR-Cas Systems*
Clustered Regularly Interspaced Short Palindromic Repeats*
Gene Editing*
Gene Knockout Techniques*
CRISPR-Associated Protein 9/*genetics
Hematopoietic Stem Cells/*metabolism
PAX5 Transcription Factor/*genetics
Animals ; CRISPR-Associated Protein 9/metabolism ; Cells, Cultured ; Green Fluorescent Proteins/genetics ; Green Fluorescent Proteins/metabolism ; Hematopoietic Stem Cells/immunology ; Mice, Inbred C57BL ; Mice, Transgenic ; PAX5 Transcription Factor/deficiency ; Phenotype ; RNA, Guide/genetics ; RNA, Guide/metabolism ; Transduction, Genetic
Czasopismo naukowe
Tytuł :
Generation of Transgenic Rat Embryonic Stem Cells Using the CRISPR/Cpf1 System for Inducible Gene Knockout.
Autorzy :
Sherstyuk VV; Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, 630090, Russia. .
Zakian SM; Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, 630090, Russia.
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Źródło :
Biochemistry. Biokhimiia [Biochemistry (Mosc)] 2021 Jul; Vol. 86 (7), pp. 843-851.
Typ publikacji :
Journal Article
MeSH Terms :
Embryonic Stem Cells*
Gene Editing*
Gene Knockout Techniques*
Rats, Transgenic*
Animals ; CRISPR-Cas Systems ; Integrases ; Rats ; Tamoxifen/analogs & derivatives
Czasopismo naukowe
Tytuł :
Genetic Ablation of Transmembrane Prolyl 4-Hydroxylase Reduces Atherosclerotic Plaques in Mice.
Autorzy :
Määttä J; Biocenter Oulu, Faculty of Biochemistry and Molecular Medicine, Oulu Center for Cell-Matrix Research (J. Määttä, R.S., V.I., J. Myllyharju, E.Y.D., P.K.), University of Oulu, Finland.
Serpi R; Biocenter Oulu, Faculty of Biochemistry and Molecular Medicine, Oulu Center for Cell-Matrix Research (J. Määttä, R.S., V.I., J. Myllyharju, E.Y.D., P.K.), University of Oulu, Finland.
Hörkkö S; Institute of Biomedicine (S.H.), University of Oulu, Finland.
Izzi V; Faculty of Medicine (V.I.), University of Oulu, Finland.; Finnish Cancer Institute, Helsinki, Finland (V.I.).
Myllyharju J; Biocenter Oulu, Faculty of Biochemistry and Molecular Medicine, Oulu Center for Cell-Matrix Research (J. Määttä, R.S., V.I., J. Myllyharju, E.Y.D., P.K.), University of Oulu, Finland.
Dimova EY; Biocenter Oulu, Faculty of Biochemistry and Molecular Medicine, Oulu Center for Cell-Matrix Research (J. Määttä, R.S., V.I., J. Myllyharju, E.Y.D., P.K.), University of Oulu, Finland.
Koivunen P; Biocenter Oulu, Faculty of Biochemistry and Molecular Medicine, Oulu Center for Cell-Matrix Research (J. Määttä, R.S., V.I., J. Myllyharju, E.Y.D., P.K.), University of Oulu, Finland.
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Źródło :
Arteriosclerosis, thrombosis, and vascular biology [Arterioscler Thromb Vasc Biol] 2021 Jul; Vol. 41 (7), pp. 2128-2140. Date of Electronic Publication: 2021 May 27.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Gene Knockout Techniques*
Plaque, Atherosclerotic*
Aorta/*enzymology
Aortic Diseases/*prevention & control
Atherosclerosis/*prevention & control
Liver/*enzymology
Prolyl Hydroxylases/*deficiency
Animals ; Aorta/pathology ; Aortic Diseases/enzymology ; Aortic Diseases/genetics ; Aortic Diseases/pathology ; Atherosclerosis/enzymology ; Atherosclerosis/genetics ; Atherosclerosis/pathology ; Autoantibodies/blood ; Diet, High-Fat ; Disease Models, Animal ; Lipoprotein Lipase/metabolism ; Lipoproteins, LDL/immunology ; Lipoproteins, LDL/metabolism ; Male ; Mice, Inbred C57BL ; Mice, Knockout ; Prolyl Hydroxylases/genetics ; Receptors, LDL/genetics ; Receptors, LDL/metabolism ; Transcriptome ; Triglycerides/blood
Czasopismo naukowe
Tytuł :
Knockout of CD147 inhibits the proliferation, invasion, and drug resistance of human oral cancer CAL27 cells in Vitro and in Vivo.
Autorzy :
Pan S; Department of Biopharmacy, School of Pharmaceutical Sciences, Jilin University, Chang Chun, China.
Su Y; Department of Biopharmacy, School of Pharmaceutical Sciences, Jilin University, Chang Chun, China.
Sun B; Department of Biopharmacy, School of Pharmaceutical Sciences, Jilin University, Chang Chun, China.
Hao R; Department of Biopharmacy, School of Pharmaceutical Sciences, Jilin University, Chang Chun, China.
Gao X; Department of Biopharmacy, School of Pharmaceutical Sciences, Jilin University, Chang Chun, China.
Han B; Department of Biopharmacy, School of Pharmaceutical Sciences, Jilin University, Chang Chun, China. Electronic address: .
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Źródło :
International journal of biological macromolecules [Int J Biol Macromol] 2021 Jun 30; Vol. 181, pp. 378-389. Date of Electronic Publication: 2021 Mar 23.
Typ publikacji :
Journal Article
MeSH Terms :
Drug Resistance, Neoplasm*/drug effects
Gene Knockout Techniques*
Basigin/*metabolism
Mouth Neoplasms/*drug therapy
Mouth Neoplasms/*pathology
Animals ; Antineoplastic Agents/pharmacology ; Antineoplastic Agents/therapeutic use ; Apoptosis/drug effects ; CRISPR-Cas Systems/genetics ; Cell Line, Tumor ; Cell Movement/drug effects ; Cell Proliferation/drug effects ; Disease Models, Animal ; Doxorubicin/pharmacology ; Doxorubicin/therapeutic use ; Humans ; Matrix Metalloproteinases/metabolism ; Mice, Nude ; Neoplasm Invasiveness
Czasopismo naukowe
Tytuł :
ITGB6 -Knockout Suppresses Cholangiocarcinoma Cell Migration and Invasion with Declining PODXL2 Expression.
Autorzy :
Soejima Y; Department of Molecular Pathology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan.
Takeuchi M; Department of Molecular Pathology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan.
Miyamoto N; Department of Molecular Pathology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan.
Sawabe M; Department of Molecular Pathology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan.
Fukusato T; General Medical Education and Research Center, Teikyo University, 2-11-1 Kaga, Itabashi-ku, Tokyo 178-8605, Japan.
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Źródło :
International journal of molecular sciences [Int J Mol Sci] 2021 Jun 11; Vol. 22 (12). Date of Electronic Publication: 2021 Jun 11.
Typ publikacji :
Journal Article
MeSH Terms :
Cell Movement*/genetics
Gene Knockout Techniques*
Cell Adhesion Molecules/*metabolism
Cholangiocarcinoma/*pathology
Integrin beta Chains/*genetics
Sialoglycoproteins/*metabolism
Adult ; Aged ; Aged, 80 and over ; Calcium-Binding Proteins/metabolism ; Cell Adhesion Molecules/genetics ; Cell Cycle ; Cell Line, Tumor ; Cholangiocarcinoma/genetics ; Female ; Gene Expression Regulation, Neoplastic ; Humans ; Integrin beta Chains/metabolism ; Male ; Middle Aged ; Neoplasm Invasiveness ; Neoplasm Proteins/metabolism ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Sialoglycoproteins/genetics ; Tumor Stem Cell Assay ; Up-Regulation/genetics
Czasopismo naukowe
Tytuł :
Knockout of the CMP-Sialic Acid Transporter SLC35A1 in Human Cell Lines Increases Transduction Efficiency of Adeno-Associated Virus 9: Implications for Gene Therapy Potency Assays.
Autorzy :
Banning A; Institute of Biochemistry, Medical Faculty, University of Giessen, Friedrichstrasse 24, D-35392 Giessen, Germany.
Zakrzewicz A; Institute of Biochemistry, Medical Faculty, University of Giessen, Friedrichstrasse 24, D-35392 Giessen, Germany.
Chen X; Department of Pediatrics, UTSW Medical Center, Dallas, TX 75390, USA.
Gray SJ; Department of Pediatrics, UTSW Medical Center, Dallas, TX 75390, USA.
Tikkanen R; Institute of Biochemistry, Medical Faculty, University of Giessen, Friedrichstrasse 24, D-35392 Giessen, Germany.
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Źródło :
Cells [Cells] 2021 May 19; Vol. 10 (5). Date of Electronic Publication: 2021 May 19.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Gene Knockout Techniques*
Genetic Therapy*
Transduction, Genetic*
Aspartylglucosylaminase/*genetics
Dependovirus/*genetics
Neuronal Ceroid-Lipofuscinoses/*therapy
Nucleotide Transport Proteins/*genetics
Aspartylglucosylaminase/metabolism ; Genetic Vectors ; HEK293 Cells ; HeLa Cells ; Humans ; Neuronal Ceroid-Lipofuscinoses/enzymology ; Neuronal Ceroid-Lipofuscinoses/genetics ; Nucleotide Transport Proteins/metabolism
Czasopismo naukowe
Tytuł :
CRISPR/Cas9-Mediated Knockout of GmFATB1 Significantly Reduced the Amount of Saturated Fatty Acids in Soybean Seeds.
Autorzy :
Ma J; State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058, China.; The Provincial International Science and Technology Cooperation Base on Engineering Biology, International Campus of Zhejiang University, Haining 314400, China.
Sun S; State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058, China.; The Provincial International Science and Technology Cooperation Base on Engineering Biology, International Campus of Zhejiang University, Haining 314400, China.
Whelan J; State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058, China.; The Provincial International Science and Technology Cooperation Base on Engineering Biology, International Campus of Zhejiang University, Haining 314400, China.; Australian Research Council Centre of Excellence in Plant Energy Biology, Department of Animal, Plant and Soil Science, School of Life Science, La Trobe University, Bundoora, VIC 3086, Australia.
Shou H; State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058, China.; The Provincial International Science and Technology Cooperation Base on Engineering Biology, International Campus of Zhejiang University, Haining 314400, China.; Hainan Institute, Zhejiang University, Sanya 572025, China.
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Źródło :
International journal of molecular sciences [Int J Mol Sci] 2021 Apr 09; Vol. 22 (8). Date of Electronic Publication: 2021 Apr 09.
Typ publikacji :
Journal Article
MeSH Terms :
CRISPR-Cas Systems*
Gene Knockout Techniques*
Gene Targeting*
Fatty Acids/*metabolism
Soybeans/*genetics
Soybeans/*metabolism
Thiolester Hydrolases/*deficiency
Gene Expression ; Genetic Association Studies ; Genetic Engineering ; Humans ; Mutation ; Phenotype ; Plant Proteins/genetics ; Soybean Oil/genetics ; Soybean Oil/metabolism
Czasopismo naukowe
Tytuł :
Knockout of zebrafish desmin genes does not cause skeletal muscle degeneration but alters calcium flux.
Autorzy :
Kayman Kürekçi G; Department of Medical Biology, Hacettepe University Faculty of Medicine, 06100, Ankara, Turkey.
Kural Mangit E; Department of Medical Biology, Hacettepe University Faculty of Medicine, 06100, Ankara, Turkey.; Laboratory Animals Research and Application Centre, Hacettepe University, 06100, Ankara, Turkey.
Koyunlar C; Department of Medical Biology, Hacettepe University Faculty of Medicine, 06100, Ankara, Turkey.; Department of Hematology, Erasmus MC, 3015 CN, Rotterdam, The Netherlands.
Unsal S; Department of Medical Biology, Hacettepe University Faculty of Medicine, 06100, Ankara, Turkey.; Department of Cancer and Inflammation, The Institute of Molecular Medicine, University of Southern Denmark, 5000, Odense C, Denmark.
Saglam B; Department of Biophysics, Hacettepe University Faculty of Medicine, 06100, Ankara, Turkey.
Ergin B; Department of Biophysics, Hacettepe University Faculty of Medicine, 06100, Ankara, Turkey.
Gizer M; Department of Stem Cell Sciences, Hacettepe University Graduate School of Health Sciences, 06100, Ankara, Turkey.
Uyanik I; Department of Electrical and Electronics Engineering, Hacettepe University Faculty of Engineering, 06800, Ankara, Turkey.
Boustanabadimaralan Düz N; Department of Medical Biology, Hacettepe University Faculty of Medicine, 06100, Ankara, Turkey.
Korkusuz P; Department of Histology and Embryology, Hacettepe University Faculty of Medicine, 06100, Ankara, Turkey.
Talim B; Pathology Unit, Department of Pediatrics, Hacettepe University Faculty of Medicine, 06100, Ankara, Turkey.
Purali N; Department of Biophysics, Hacettepe University Faculty of Medicine, 06100, Ankara, Turkey.
Hughes SM; Randall Centre for Cell and Molecular Biophysics, New Hunt's House, Guy's Campus, King's College London, London, SE1 1UL, United Kingdom.
Dincer PR; Department of Medical Biology, Hacettepe University Faculty of Medicine, 06100, Ankara, Turkey. .
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Źródło :
Scientific reports [Sci Rep] 2021 Apr 05; Vol. 11 (1), pp. 7505. Date of Electronic Publication: 2021 Apr 05.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Gene Knockout Techniques*
Calcium/*metabolism
Desmin/*genetics
Muscle, Skeletal/*metabolism
Muscle, Skeletal/*pathology
Zebrafish/*genetics
Animals ; Base Sequence ; Desmin/metabolism ; Embryo, Nonmammalian/metabolism ; Gene Expression Profiling ; Gene Expression Regulation, Developmental ; Larva/genetics ; Muscle Fibers, Skeletal/pathology ; Muscle, Skeletal/ultrastructure ; Mutation/genetics ; Neuromuscular Junction/pathology ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Zebrafish/embryology
Czasopismo naukowe
Tytuł :
Synergizing Off-Target Predictions for In Silico Insights of CENH3 Knockout in Cannabis through CRISPR/Cas.
Autorzy :
Hesami M; Department of Plant Agriculture, University of Guelph, Guelph, ON N1G 2W1, Canada.
Yoosefzadeh Najafabadi M; Department of Plant Agriculture, University of Guelph, Guelph, ON N1G 2W1, Canada.
Adamek K; Department of Plant Agriculture, University of Guelph, Guelph, ON N1G 2W1, Canada.
Torkamaneh D; Department of Plant Agriculture, University of Guelph, Guelph, ON N1G 2W1, Canada.; Département de Phytologie, Université Laval, Québec City, QC G1V 0A6, Canada.
Jones AMP; Department of Plant Agriculture, University of Guelph, Guelph, ON N1G 2W1, Canada.
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Źródło :
Molecules (Basel, Switzerland) [Molecules] 2021 Apr 03; Vol. 26 (7). Date of Electronic Publication: 2021 Apr 03.
Typ publikacji :
Journal Article
MeSH Terms :
Computer Simulation*
Gene Knockout Techniques*
CRISPR-Cas Systems/*genetics
Cannabis/*genetics
Centromere/*metabolism
Histones/*genetics
Area Under Curve ; ROC Curve ; Support Vector Machine
Czasopismo naukowe
Tytuł :
Adipose-specific knockout of ubiquitin-conjugating enzyme E2L6 (Ube2l6) reduces diet-induced obesity, insulin resistance, and hepatic steatosis.
Autorzy :
Wei W; Department of Endocrinology, Hainan General Hospital, Haikou, China.
Li Y; Hainan Provincial Healthcare Center, Hainan General Hospital, Haikou, China.
Li Y; Chuangxu Institute of Life Science, Chongqing, China.
Li D; Department of Urological Surgery, Hainan General Hospital, Haikou, China. Electronic address: .
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Źródło :
Journal of pharmacological sciences [J Pharmacol Sci] 2021 Apr; Vol. 145 (4), pp. 327-334. Date of Electronic Publication: 2020 Dec 30.
Typ publikacji :
Journal Article
MeSH Terms :
Gene Knockout Techniques*
Adipocytes/*metabolism
Adipogenesis/*genetics
Diet, High-Fat/*adverse effects
Fatty Liver/*genetics
Insulin Resistance/*genetics
Obesity/*etiology
Obesity/*genetics
Ubiquitin-Conjugating Enzymes/*genetics
Ubiquitin-Conjugating Enzymes/*physiology
Animals ; Humans ; Lipase/genetics ; Lipase/metabolism ; Lipase/physiology ; Mice ; Ubiquitination/drug effects
Czasopismo naukowe
Tytuł :
CRISPR/Cas9 generated knockout mice lacking phenylalanine hydroxylase protein as a novel preclinical model for human phenylketonuria.
Autorzy :
Singh K; Global Discovery Pathology, Translational In-Vivo Models Research Platform, Sanofi, 5 The Mountain Road, Framingham, MA, 01701, USA.; WuXi AppTec Inc., 8th Floor, 55 Cambridge Parkway, Cambridge, MA, 02142, USA.
Cornell CS; Genomic Medicine Unit, Sanofi, 49 New York Avenue, Framingham, MA, 01701, USA.
Jackson R; Genomic Medicine Unit, Sanofi, 49 New York Avenue, Framingham, MA, 01701, USA.
Kabiri M; Transgenic Model and Technology, Translational In-Vivo Research Platform, Industrie Park Hoechst, Sanofi, Frankfurt, Germany.
Phipps M; Transgenic Model and Technology, Translational In-Vivo Models Research Platform, Sanofi, 5 The Mountain Road, Framingham, MA, 01701, USA.
Desai M; Global Bioimaging, Translational In-Vivo Models Research Platform, Sanofi, Framingham, MA, 01701, USA.
Fogle R; Global Bioimaging, Translational In-Vivo Models Research Platform, Sanofi, Framingham, MA, 01701, USA.
Ying X; Global Bioimaging, Translational In-Vivo Models Research Platform, Sanofi, Framingham, MA, 01701, USA.
Anarat-Cappillino G; Pre-Development Sciences NA, Analytical R&D, Sanofi, Framingham, MA, 01701, USA.
Geller S; Pre-Development Sciences NA, Analytical R&D, Sanofi, Framingham, MA, 01701, USA.
Johnson J; Global Discovery Pathology, Translational In-Vivo Models Research Platform, Sanofi, 5 The Mountain Road, Framingham, MA, 01701, USA.
Roberts E; Global Discovery Pathology, Translational In-Vivo Models Research Platform, Sanofi, 5 The Mountain Road, Framingham, MA, 01701, USA.
Malley K; Global Discovery Pathology, Translational In-Vivo Models Research Platform, Sanofi, 5 The Mountain Road, Framingham, MA, 01701, USA.
Devlin T; Transgenic Model and Technology, Translational In-Vivo Models Research Platform, Sanofi, 5 The Mountain Road, Framingham, MA, 01701, USA.
DeRiso M; Transgenic Model and Technology, Translational In-Vivo Models Research Platform, Sanofi, 5 The Mountain Road, Framingham, MA, 01701, USA.
Berthelette P; Genomic Medicine Unit, Sanofi, 49 New York Avenue, Framingham, MA, 01701, USA.
Zhang YV; Genomic Medicine Unit, Sanofi, 49 New York Avenue, Framingham, MA, 01701, USA.
Ryan S; Global Discovery Pathology, Translational In-Vivo Models Research Platform, Sanofi, 5 The Mountain Road, Framingham, MA, 01701, USA.
Rao S; Translational In-Vivo Models Research Platform, Sanofi, 49 New York Avenue, Framingham, MA, 01701, USA.
Thurberg BL; Global Discovery Pathology, Translational In-Vivo Models Research Platform, Sanofi, 5 The Mountain Road, Framingham, MA, 01701, USA.
Bangari DS; Global Discovery Pathology, Translational In-Vivo Models Research Platform, Sanofi, 5 The Mountain Road, Framingham, MA, 01701, USA. .
Kyostio-Moore S; Genomic Medicine Unit, Sanofi, 49 New York Avenue, Framingham, MA, 01701, USA. .
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Źródło :
Scientific reports [Sci Rep] 2021 Mar 31; Vol. 11 (1), pp. 7254. Date of Electronic Publication: 2021 Mar 31.
Typ publikacji :
Journal Article
MeSH Terms :
CRISPR-Cas Systems*
Disease Models, Animal*
Gene Knockout Techniques*
Phenylalanine Hydroxylase/*genetics
Phenylketonurias/*genetics
Animals ; Male ; Mice ; Mice, Knockout
Czasopismo naukowe
Tytuł :
Flow Assisted Mutation Enrichment (FAME): A highly efficacious and efficient method to enrich Double Knockouts (DKO) after gene editing.
Autorzy :
Hansen M; Division of Endocrinology, Phoenix VA Medical Center, Phoenix, Arizona, United States of America.
Cai X; Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, Minnesota, United States of America.
Bowen S; Flow Cytometry Core Facility, Barrow Neurological Institute, Phoenix, Arizona, United States of America.
Largaespada DA; Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, Minnesota, United States of America.; Department of Pediatrics, University of Minnesota, Minneapolis, Minnesota, United States of America.
Li MV; Division of Endocrinology, Phoenix VA Medical Center, Phoenix, Arizona, United States of America.; Department of Medicine, University of Arizona College of Medicine, Phoenix, Arizona, United States of America.
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Źródło :
PloS one [PLoS One] 2021 Mar 04; Vol. 16 (3), pp. e0247375. Date of Electronic Publication: 2021 Mar 04 (Print Publication: 2021).
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
MeSH Terms :
Flow Cytometry*
Gene Editing*
Gene Knockout Techniques*
HEK293 Cells ; Humans
Czasopismo naukowe
Tytuł :
Altered Visual Function in a Larval Zebrafish Knockout of Neurodevelopmental Risk Gene pdzk1.
Autorzy :
Xie J; School of BioSciences, The University of Melbourne, Melbourne, Australia.
Jusuf PR; School of BioSciences, The University of Melbourne, Melbourne, Australia.
Bui BV; Department of Optometry and Vision Sciences, The University of Melbourne, Melbourne, Australia.
Dudczig S; School of BioSciences, The University of Melbourne, Melbourne, Australia.
Sztal TE; School of Biological Sciences, Monash University, Melbourne, Australia.
Goodbourn PT; Melbourne School of Psychological Sciences, The University of Melbourne, Melbourne, Australia.
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Źródło :
Investigative ophthalmology & visual science [Invest Ophthalmol Vis Sci] 2021 Mar 01; Vol. 62 (3), pp. 29.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Gene Knockout Techniques*
PDZ Domains/*genetics
Psychomotor Performance/*physiology
Retina/*physiopathology
Vision Disorders/*genetics
Zebrafish Proteins/*genetics
Animals ; CRISPR-Associated Protein 9 ; Contrast Sensitivity/physiology ; Electroretinography ; Genotyping Techniques ; Larva ; Real-Time Polymerase Chain Reaction ; Receptors, Corticotropin-Releasing Hormone/metabolism ; Receptors, N-Methyl-D-Aspartate/metabolism ; Retina/metabolism ; Serotonin/metabolism ; Vision Disorders/metabolism ; Vision Disorders/physiopathology ; Zebrafish
Czasopismo naukowe
Tytuł :
Generation of Knockout and Transgenic Zebrafish to Characterize Abcc4 Functions in Detoxification and Efflux of Lead.
Autorzy :
Lu X; Key Laboratory of Freshwater Biodiversity Conservation, Ministry of Agriculture and Rural Affairs, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China.; Department of Genetics, Wuhan University, Wuhan 430071, China.
Long Y; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
Li X; Department of Genetics, Wuhan University, Wuhan 430071, China.; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.; Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, State Key Laboratory of Applied Microbiology Southern China, Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China.
Zhang L; Department of Genetics, Wuhan University, Wuhan 430071, China.
Li Q; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
Wen H; Key Laboratory of Freshwater Biodiversity Conservation, Ministry of Agriculture and Rural Affairs, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China.
Zhong S; Department of Genetics, Wuhan University, Wuhan 430071, China.; Hubei Provincial Key Laboratory of Allergy and Immunology, Wuhan 430071, China.
Cui Z; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.; Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, State Key Laboratory of Applied Microbiology Southern China, Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China.
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Źródło :
International journal of molecular sciences [Int J Mol Sci] 2021 Feb 19; Vol. 22 (4). Date of Electronic Publication: 2021 Feb 19.
Typ publikacji :
Journal Article
MeSH Terms :
Gene Knockout Techniques*
Lead/*metabolism
Multidrug Resistance-Associated Proteins/*metabolism
Zebrafish/*genetics
Zebrafish Proteins/*metabolism
Animals ; Animals, Genetically Modified ; Base Sequence ; Biological Transport ; Gene Expression Regulation, Developmental ; Inactivation, Metabolic ; LLC-PK1 Cells ; Multidrug Resistance-Associated Proteins/genetics ; Mutation/genetics ; Swine ; Zebrafish/embryology ; Zebrafish Proteins/genetics
Czasopismo naukowe
Tytuł :
Non-Transgenic CRISPR-Mediated Knockout of Entire Ergot Alkaloid Gene Clusters in Slow-Growing Asexual Polyploid Fungi.
Autorzy :
Florea S; Department of Plant Pathology, University of Kentucky, Lexington, KY 40546, USA.
Jaromczyk J; Computer Science Department, University of Kentucky, Lexington, KY 40546, USA.
Schardl CL; Department of Plant Pathology, University of Kentucky, Lexington, KY 40546, USA.
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Źródło :
Toxins [Toxins (Basel)] 2021 Feb 16; Vol. 13 (2). Date of Electronic Publication: 2021 Feb 16.
Typ publikacji :
Journal Article; Research Support, U.S. Gov't, Non-P.H.S.
MeSH Terms :
CRISPR-Cas Systems*
Gene Editing*
Gene Knockout Techniques*
Multigene Family*
Endophytes/*genetics
Epichloe/*genetics
Ergot Alkaloids/*genetics
CRISPR-Associated Protein 9/genetics ; CRISPR-Associated Protein 9/metabolism ; Clustered Regularly Interspaced Short Palindromic Repeats ; Endophytes/growth & development ; Endophytes/metabolism ; Epichloe/growth & development ; Epichloe/metabolism ; Ergot Alkaloids/biosynthesis ; Ergot Alkaloids/toxicity ; Gene Expression Regulation, Fungal ; Reproduction, Asexual ; Secondary Metabolism
SCR Organism :
Epichloe coenophiala
Czasopismo naukowe
Tytuł :
Brain iron deficiency and affected contextual fear memory in mice with conditional Ferroportin1 ablation in the brain.
Autorzy :
Wu Q; Laboratory of Molecular Iron Metabolism, College of Life Science, Hebei Normal University, Shijiazhuang, China.; College of Basic Medicine, Hebei University of Chinese Medicine, Shijiazhuang, China.; Hebei Key Laboratory of Chinese Medicine Research on Cardio-Cerebrovascular Disease, Shijiazhuang, China.
Hao Q; Laboratory of Molecular Iron Metabolism, College of Life Science, Hebei Normal University, Shijiazhuang, China.
Li H; Laboratory of Molecular Iron Metabolism, College of Life Science, Hebei Normal University, Shijiazhuang, China.
Wang B; Laboratory of Molecular Iron Metabolism, College of Life Science, Hebei Normal University, Shijiazhuang, China.
Wang P; Laboratory of Molecular Iron Metabolism, College of Life Science, Hebei Normal University, Shijiazhuang, China.
Jin X; Laboratory of Molecular Iron Metabolism, College of Life Science, Hebei Normal University, Shijiazhuang, China.
Yu P; Laboratory of Molecular Iron Metabolism, College of Life Science, Hebei Normal University, Shijiazhuang, China.
Gao G; Laboratory of Molecular Iron Metabolism, College of Life Science, Hebei Normal University, Shijiazhuang, China.
Chang YZ; Laboratory of Molecular Iron Metabolism, College of Life Science, Hebei Normal University, Shijiazhuang, China.
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Źródło :
FASEB journal : official publication of the Federation of American Societies for Experimental Biology [FASEB J] 2021 Feb; Vol. 35 (2), pp. e21174. Date of Electronic Publication: 2020 Nov 16.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Fear*
Gene Knockout Techniques*
Memory*
Blood-Brain Barrier/*metabolism
Cation Transport Proteins/*genetics
Hippocampus/*metabolism
Iron/*deficiency
Animals ; Astrocytes/metabolism ; Cation Transport Proteins/metabolism ; Cells, Cultured ; Endothelial Cells/metabolism ; Homeostasis/genetics ; Male ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Neurons/metabolism ; Stress Disorders, Post-Traumatic/metabolism
Czasopismo naukowe
Tytuł :
Muscle-specific deletion of SLK/Stk2 enhances p38 activity and myogenesis in mdx mice.
Autorzy :
Pryce BR; Department of Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa, Ottawa, ON, Canada; Cancer Therapeutics Program, Ottawa Hospital Research Institute, Ottawa, ON, Canada.
Labrèche C; Department of Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa, Ottawa, ON, Canada; Cancer Therapeutics Program, Ottawa Hospital Research Institute, Ottawa, ON, Canada.
Hamoudi D; Centre de Recherche du Centre Hospitalier Universitaire de Québec-Centre Hospitalier de L'Université Laval, Université Laval, Quebec City, Quebec, Canada.
Abou-Hamad J; Department of Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa, Ottawa, ON, Canada; Cancer Therapeutics Program, Ottawa Hospital Research Institute, Ottawa, ON, Canada.
Al-Zahrani KN; Department of Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa, Ottawa, ON, Canada; Cancer Therapeutics Program, Ottawa Hospital Research Institute, Ottawa, ON, Canada.
Hodgins JJ; Cancer Therapeutics Program, Ottawa Hospital Research Institute, Ottawa, ON, Canada; Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, Ottawa, ON, Canada.
Boulanger-Piette A; Centre de Recherche du Centre Hospitalier Universitaire de Québec-Centre Hospitalier de L'Université Laval, Université Laval, Quebec City, Quebec, Canada.
Bossé S; Centre de Recherche du Centre Hospitalier Universitaire de Québec-Centre Hospitalier de L'Université Laval, Université Laval, Quebec City, Quebec, Canada.
Balog-Alvarez C; Department of Veterinary Integrative Biosciences, Texas A&M University College of Veterinary Medicine & Biomedical Sciences, College Station, TX, USA.
Frénette J; Centre de Recherche du Centre Hospitalier Universitaire de Québec-Centre Hospitalier de L'Université Laval, Université Laval, Quebec City, Quebec, Canada; Département de Réadaptation, Faculté de Médecine, Université Laval, Quebec City, Quebec, Canada.
Ardolino M; Cancer Therapeutics Program, Ottawa Hospital Research Institute, Ottawa, ON, Canada; Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, Ottawa, ON, Canada.
Kornegay JN; Department of Veterinary Integrative Biosciences, Texas A&M University College of Veterinary Medicine & Biomedical Sciences, College Station, TX, USA.
Sabourin LA; Department of Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa, Ottawa, ON, Canada; Cancer Therapeutics Program, Ottawa Hospital Research Institute, Ottawa, ON, Canada. Electronic address: .
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Źródło :
Biochimica et biophysica acta. Molecular cell research [Biochim Biophys Acta Mol Cell Res] 2021 Feb; Vol. 1868 (2), pp. 118917. Date of Electronic Publication: 2020 Nov 28.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Gene Knockout Techniques*
MAP Kinase Signaling System/*genetics
Muscle Development/*genetics
Muscle, Skeletal/*metabolism
Muscular Dystrophy, Duchenne/*metabolism
Protein-Serine-Threonine Kinases/*metabolism
p38 Mitogen-Activated Protein Kinases/*metabolism
Animals ; Cells, Cultured ; Disease Models, Animal ; Dogs ; Mice ; Mice, Inbred mdx ; Mice, Knockout ; Muscular Dystrophy, Duchenne/pathology ; Myoblasts/metabolism ; Myogenin/metabolism ; Protein-Serine-Threonine Kinases/genetics ; Transforming Growth Factor beta/metabolism
Czasopismo naukowe

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