Temat: "Genes, Reporter" - OpacWWW

Skocz do pozycji: 1 1.

Tytuł :: Analysis of SARS-CoV-2 infection dynamic in vivo using reporter-expressing viruses.
Autorzy :: Ye C; Host-Pathogen Interactions Program, Texas Biomedical Research Institute, San Antonio, TX 78227; .; Population Health Program, Texas Biomedical Research Institute, San Antonio, TX 78227.
Chiem K; Host-Pathogen Interactions Program, Texas Biomedical Research Institute, San Antonio, TX 78227.; Population Health Program, Texas Biomedical Research Institute, San Antonio, TX 78227.
Park JG; Host-Pathogen Interactions Program, Texas Biomedical Research Institute, San Antonio, TX 78227.; Population Health Program, Texas Biomedical Research Institute, San Antonio, TX 78227.
Silvas JA; Host-Pathogen Interactions Program, Texas Biomedical Research Institute, San Antonio, TX 78227.; Population Health Program, Texas Biomedical Research Institute, San Antonio, TX 78227.
Morales Vasquez D; Host-Pathogen Interactions Program, Texas Biomedical Research Institute, San Antonio, TX 78227.; Population Health Program, Texas Biomedical Research Institute, San Antonio, TX 78227.
Sourimant J; Center for Translational Antiviral Research, Institute for Biomedical Sciences, Georgia State University, Atlanta, GA 30303.
Lin MJ; Virology Division, Department of Laboratory Medicine, University of Washington, Seattle, WA 98195.
Greninger AL; Virology Division, Department of Laboratory Medicine, University of Washington, Seattle, WA 98195.
Plemper RK; Center for Translational Antiviral Research, Institute for Biomedical Sciences, Georgia State University, Atlanta, GA 30303.
Torrelles JB; Host-Pathogen Interactions Program, Texas Biomedical Research Institute, San Antonio, TX 78227.; Population Health Program, Texas Biomedical Research Institute, San Antonio, TX 78227.
Kobie JJ; Department of Medicine, Division of Infectious Diseases, University of Alabama at Birmingham, Birmingham, AL 35294.
Walter MR; Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL 35294.
de la Torre JC; Department of Immunology and Microbiology, The Scripps Research Institute, La Jolla, CA 92037.
Martinez-Sobrido L; Host-Pathogen Interactions Program, Texas Biomedical Research Institute, San Antonio, TX 78227; .; Population Health Program, Texas Biomedical Research Institute, San Antonio, TX 78227.; Pokaż więcej
Źródło :: Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2021 Oct 12; Vol. 118 (41).
Typ publikacji :: Journal Article
MeSH Terms :: COVID-19*/genetics
COVID-19*/metabolism
Gene Expression Regulation, Viral*
Genes, Reporter*
SARS-CoV-2*/genetics
SARS-CoV-2*/metabolism
Viral Proteins*/biosynthesis
Viral Proteins*/genetics
Angiotensin-Converting Enzyme 2/genetics ; Angiotensin-Converting Enzyme 2/metabolism ; Animals ; Chlorocebus aethiops ; Coronavirus Nucleocapsid Proteins/biosynthesis ; Coronavirus Nucleocapsid Proteins/genetics ; Female ; Humans ; Mice ; Mice, Transgenic ; Teschovirus/genetics ; Vero Cells
: Czasopismo naukowe

Szczegóły

Skocz do pozycji: 2 2.

Tytuł :: CG Dinucleotide Removal in Bioluminescent and Fluorescent Reporters Improves HIV-1 Replication and Reporter Gene Expression for Dual Imaging in Humanized Mice.
Autorzy :: Roy CN; Department of Microbiology and Molecular Genetics, University of Pittsburghgrid.21925.3d School of Medicine, Pittsburgh, Pennsylvania, USA.
Benitez Moreno MA; Department of Infectious Diseases and Microbiology, University of Pittsburghgrid.21925.3d Graduate School of Public Health, Pittsburgh, Pennsylvania, USA.
Kline C; Department of Microbiology and Molecular Genetics, University of Pittsburghgrid.21925.3d School of Medicine, Pittsburgh, Pennsylvania, USA.
Ambrose Z; Department of Microbiology and Molecular Genetics, University of Pittsburghgrid.21925.3d School of Medicine, Pittsburgh, Pennsylvania, USA.; Department of Infectious Diseases and Microbiology, University of Pittsburghgrid.21925.3d Graduate School of Public Health, Pittsburgh, Pennsylvania, USA.; Pokaż więcej
Źródło :: Journal of virology [J Virol] 2021 Sep 09; Vol. 95 (19), pp. e0044921. Date of Electronic Publication: 2021 Sep 09.
Typ publikacji :: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
MeSH Terms :: Genes, Reporter*
Virus Replication*
Dinucleoside Phosphates/*metabolism
HIV Infections/*virology
HIV-1/*physiology
Animals ; CD4-Positive T-Lymphocytes/virology ; Gene Expression ; HIV-1/genetics ; Humans ; Luciferases/genetics ; Luminescent Measurements ; Luminescent Proteins/genetics ; Mice ; Optical Imaging ; Viremia ; Whole Body Imaging
: Czasopismo naukowe

Szczegóły

Skocz do pozycji: 3 3.

Tytuł :: Light-Inducible Spatio-Temporal Control of TLR4 and NF-κB-Gluc Reporter in Human Pancreatic Cell Line.
Autorzy :: Stierschneider A; Department of Medical and Pharmaceutical Biotechnology, IMC University of Applied Sciences, 3500 Krems, Austria.
Grünstäudl P; Department of Medical and Pharmaceutical Biotechnology, IMC University of Applied Sciences, 3500 Krems, Austria.
Colleselli K; Department of Medical and Pharmaceutical Biotechnology, IMC University of Applied Sciences, 3500 Krems, Austria.
Atzler J; Molecular Devices, LLC, 5071 Wals-Siezenheim, Austria.
Klein CT; Department of Medical and Pharmaceutical Biotechnology, IMC University of Applied Sciences, 3500 Krems, Austria.
Hundsberger H; Department of Medical and Pharmaceutical Biotechnology, IMC University of Applied Sciences, 3500 Krems, Austria.
Wiesner C; Department of Medical and Pharmaceutical Biotechnology, IMC University of Applied Sciences, 3500 Krems, Austria.; Pokaż więcej
Źródło :: International journal of molecular sciences [Int J Mol Sci] 2021 Aug 26; Vol. 22 (17). Date of Electronic Publication: 2021 Aug 26.
Typ publikacji :: Journal Article
MeSH Terms :: Genes, Reporter*
Light*
NF-kappa B/*metabolism
Optogenetics/*methods
Toll-Like Receptor 4/*metabolism
Cell Adhesion ; Cell Movement ; HEK293 Cells ; HeLa Cells ; Humans ; Mitogen-Activated Protein Kinase 1/metabolism ; Mitogen-Activated Protein Kinase 3/metabolism ; Oxygen/metabolism ; Pancreas/cytology ; Podosomes/metabolism ; Signal Transduction ; Toll-Like Receptor 4/genetics
: Czasopismo naukowe

Szczegóły Pełny tekst

Skocz do pozycji: 4 4.

Tytuł :: Rescue of dual reporter-tagged parainfluenza virus 5 as tool for rapid screening of antivirals in vitro.
Autorzy :: Liu F; College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, 266109, China. Electronic address: .
Wang Q; College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, 266109, China.
Shan H; College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, 266109, China. Electronic address: .; Pokaż więcej
Źródło :: Veterinary microbiology [Vet Microbiol] 2021 Aug; Vol. 259, pp. 109154. Date of Electronic Publication: 2021 Jun 16.
Typ publikacji :: Journal Article
MeSH Terms :: Genes, Reporter*
Antiviral Agents/*pharmacology
High-Throughput Screening Assays/*methods
Parainfluenza Virus 5/*drug effects
Parainfluenza Virus 5/*metabolism
Cell Line ; Cytopathogenic Effect, Viral ; Green Fluorescent Proteins/metabolism ; Virus Replication/drug effects
: Czasopismo naukowe

Szczegóły

Skocz do pozycji: 5 5.

Tytuł :: Cytosine and adenosine base editing in human pluripotent stem cells using transient reporters for editing enrichment.
Autorzy :: Tekel SJ; School for Engineering of Matter, Transport, and Energy, Arizona State University, Tempe, AZ, USA.
Brookhouser N; School of Biological and Health Systems Engineering, Arizona State University, Tempe, AZ, USA.
Standage-Beier K; School of Biological and Health Systems Engineering, Arizona State University, Tempe, AZ, USA.; Molecular and Cellular Biology Graduate Program, Arizona State University, Tempe, AZ, USA.
Wang X; School of Biological and Health Systems Engineering, Arizona State University, Tempe, AZ, USA. .
Brafman DA; School of Biological and Health Systems Engineering, Arizona State University, Tempe, AZ, USA. .; Pokaż więcej
Źródło :: Nature protocols [Nat Protoc] 2021 Jul; Vol. 16 (7), pp. 3596-3624. Date of Electronic Publication: 2021 Jun 25.
Typ publikacji :: Journal Article; Research Support, N.I.H., Extramural
MeSH Terms :: Genes, Reporter*
Adenosine/*metabolism
Cytosine/*metabolism
Gene Editing/*methods
Induced Pluripotent Stem Cells/*metabolism
Base Sequence ; Clone Cells ; Cryopreservation ; Flow Cytometry ; Humans ; Plasmids/genetics ; RNA, Guide/metabolism ; Reproducibility of Results ; Single-Cell Analysis ; Transfection
: Czasopismo naukowe

Szczegóły

Skocz do pozycji: 6 6.

Tytuł :: Chromatin-embedded reporter genes: Quantification of stimulus-induced gene transcription.
Autorzy :: Thiel G; Department of Medical Biochemistry and Molecular Biology, Saarland University Medical Faculty, D-66421 Homburg, Germany. Electronic address: .
Backes TM; Department of Medical Biochemistry and Molecular Biology, Saarland University Medical Faculty, D-66421 Homburg, Germany.
Guethlein LA; Department of Structural Biology and Department of Microbiology & Immunology, School of Medicine, Stanford University, Stanford, CA 94305, USA.
Rössler OG; Department of Medical Biochemistry and Molecular Biology, Saarland University Medical Faculty, D-66421 Homburg, Germany.; Pokaż więcej
Źródło :: Gene [Gene] 2021 Jun 30; Vol. 787, pp. 145645. Date of Electronic Publication: 2021 Apr 20.
Typ publikacji :: Journal Article; Review
MeSH Terms :: Genes, Reporter*
Transcriptional Activation*
Chromatin/*genetics
Transcription Factors/*metabolism
Animals ; Cyclic AMP Response Element-Binding Protein/metabolism ; Early Growth Response Protein 1/metabolism ; Gene Expression Regulation ; Humans ; NF-kappa B/metabolism ; NFATC Transcription Factors/metabolism ; Signal Transduction ; Transcription Factor AP-1/metabolism ; ets-Domain Protein Elk-1/metabolism
: Czasopismo naukowe

Szczegóły

Skocz do pozycji: 7 7.

Tytuł :: Evaluation of Firefly and Renilla Luciferase Inhibition in Reporter-Gene Assays: A Case of Isoflavonoids.
Autorzy :: Kenda M; Faculty of Pharmacy, University of Ljubljana, Aškerčeva Cesta 7, 1000 Ljubljana, Slovenia.
Vegelj J; Faculty of Pharmacy, University of Ljubljana, Aškerčeva Cesta 7, 1000 Ljubljana, Slovenia.
Herlah B; Faculty of Pharmacy, University of Ljubljana, Aškerčeva Cesta 7, 1000 Ljubljana, Slovenia.; National Institute of Chemistry, Hajdrihova 19, 1000 Ljubljana, Slovenia.
Perdih A; Faculty of Pharmacy, University of Ljubljana, Aškerčeva Cesta 7, 1000 Ljubljana, Slovenia.; National Institute of Chemistry, Hajdrihova 19, 1000 Ljubljana, Slovenia.
Mladěnka P; Faculty of Pharmacy in Hradec Králové, Charles University, Akademika Heyrovského 1203, 500 05 Hradec Králové, Czech Republic.
Sollner Dolenc M; Faculty of Pharmacy, University of Ljubljana, Aškerčeva Cesta 7, 1000 Ljubljana, Slovenia.; Pokaż więcej
Źródło :: International journal of molecular sciences [Int J Mol Sci] 2021 Jun 28; Vol. 22 (13). Date of Electronic Publication: 2021 Jun 28.
Typ publikacji :: Journal Article
MeSH Terms :: Genes, Reporter/*physiology
Isoflavones/*metabolism
Luciferases, Renilla/*metabolism
Animals ; Fireflies ; Genes, Reporter/genetics ; Isoflavones/chemistry ; Luciferases, Renilla/chemistry ; Protein Structure, Secondary
: Czasopismo naukowe

Szczegóły Pełny tekst Pełny tekst

Skocz do pozycji: 8 8.

Tytuł :: Live-cell imaging of rapid calcium dynamics using fluorescent, genetically-encoded GCaMP probes with Aspergillus fumigatus.
Autorzy :: Muñoz A; Manchester Fungal Infection Group, Division of Infection, Immunity and Respiratory Medicine, University of Manchester, CTF Building, 46 Grafton Street, Manchester M13 9NT, UK.
Bertuzzi M; Manchester Fungal Infection Group, Division of Infection, Immunity and Respiratory Medicine, University of Manchester, CTF Building, 46 Grafton Street, Manchester M13 9NT, UK.
Seidel C; Manchester Fungal Infection Group, Division of Infection, Immunity and Respiratory Medicine, University of Manchester, CTF Building, 46 Grafton Street, Manchester M13 9NT, UK.
Thomson D; Manchester Fungal Infection Group, Division of Infection, Immunity and Respiratory Medicine, University of Manchester, CTF Building, 46 Grafton Street, Manchester M13 9NT, UK.
Bignell EM; Manchester Fungal Infection Group, Division of Infection, Immunity and Respiratory Medicine, University of Manchester, CTF Building, 46 Grafton Street, Manchester M13 9NT, UK. Electronic address: .
Read ND; Manchester Fungal Infection Group, Division of Infection, Immunity and Respiratory Medicine, University of Manchester, CTF Building, 46 Grafton Street, Manchester M13 9NT, UK.; Pokaż więcej
Źródło :: Fungal genetics and biology : FG & B [Fungal Genet Biol] 2021 Jun; Vol. 151, pp. 103470. Date of Electronic Publication: 2020 Sep 24.
Typ publikacji :: Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :: Genes, Reporter*
Aspergillus fumigatus/*metabolism
Calcium/*metabolism
Fluorescent Dyes/*metabolism
Calcium Signaling ; Calmodulin/genetics ; Calmodulin/metabolism ; Green Fluorescent Proteins/genetics ; Green Fluorescent Proteins/metabolism ; Molecular Probes ; Myosin-Light-Chain Kinase/genetics ; Myosin-Light-Chain Kinase/metabolism ; Spores, Fungal/metabolism
: Czasopismo naukowe

Szczegóły

Skocz do pozycji: 9 9.

Tytuł :: Identification of Small Molecule Inhibitors of a Mir155 Transcriptional Reporter in Th17 Cells.
Autorzy :: Singh A; Division of Preclinical Innovation, National Center for Advancing Translational Sciences (NCATS), NIH, 9800 Medical Center Drive, Rockville, MD, 20850, USA. .
Dashynam M; Division of Preclinical Innovation, National Center for Advancing Translational Sciences (NCATS), NIH, 9800 Medical Center Drive, Rockville, MD, 20850, USA.
Chim B; Laboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases, NIH, Bethesda, MD, USA.
Escobar TM; Laboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases, NIH, Bethesda, MD, USA.
Liu X; Laboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases, NIH, Bethesda, MD, USA.
Hu X; Division of Preclinical Innovation, National Center for Advancing Translational Sciences (NCATS), NIH, 9800 Medical Center Drive, Rockville, MD, 20850, USA.
Patnaik S; Division of Preclinical Innovation, National Center for Advancing Translational Sciences (NCATS), NIH, 9800 Medical Center Drive, Rockville, MD, 20850, USA.
Xu X; Division of Preclinical Innovation, National Center for Advancing Translational Sciences (NCATS), NIH, 9800 Medical Center Drive, Rockville, MD, 20850, USA.
Southall N; Division of Preclinical Innovation, National Center for Advancing Translational Sciences (NCATS), NIH, 9800 Medical Center Drive, Rockville, MD, 20850, USA.
Marugan J; Division of Preclinical Innovation, National Center for Advancing Translational Sciences (NCATS), NIH, 9800 Medical Center Drive, Rockville, MD, 20850, USA.
Jadhav A; Division of Preclinical Innovation, National Center for Advancing Translational Sciences (NCATS), NIH, 9800 Medical Center Drive, Rockville, MD, 20850, USA.
Lazarevic V; Experimental Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.
Muljo SA; Laboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases, NIH, Bethesda, MD, USA. .
Ferrer M; Division of Preclinical Innovation, National Center for Advancing Translational Sciences (NCATS), NIH, 9800 Medical Center Drive, Rockville, MD, 20850, USA. .; Pokaż więcej
Źródło :: Scientific reports [Sci Rep] 2021 Jun 01; Vol. 11 (1), pp. 11498. Date of Electronic Publication: 2021 Jun 01.
Typ publikacji :: Journal Article; Research Support, N.I.H., Extramural
MeSH Terms :: Genes, Reporter*
Down-Regulation/*drug effects
Imidazoles/*pharmacology
MicroRNAs/*biosynthesis
Th17 Cells/*metabolism
Transcription, Genetic/*drug effects
Animals ; Cytokines/biosynthesis ; Cytokines/genetics ; Cytokines/immunology ; Down-Regulation/genetics ; Down-Regulation/immunology ; Imidazoles/chemistry ; Mice ; Mice, Transgenic ; MicroRNAs/genetics ; MicroRNAs/immunology ; Th17 Cells/immunology ; Transcription, Genetic/genetics ; Transcription, Genetic/immunology
: Czasopismo naukowe

Szczegóły Pełny tekst

Skocz do pozycji: 10 10.

Tytuł :: Modified secreted alkaline phosphatase as an improved reporter protein for N-glycosylation analysis.
Autorzy :: Olczak M; Laboratory of Biochemistry, Faculty of Biotechnology, University of Wrocław, Wrocław, Poland.
Szulc B; Laboratory of Biochemistry, Faculty of Biotechnology, University of Wrocław, Wrocław, Poland.; Pokaż więcej
Źródło :: PloS one [PLoS One] 2021 May 25; Vol. 16 (5), pp. e0251805. Date of Electronic Publication: 2021 May 25 (Print Publication: 2021).
Typ publikacji :: Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :: Genes, Reporter*
Alkaline Phosphatase/*genetics
Glycoproteins/*genetics
Alkaline Phosphatase/isolation & purification ; Alkaline Phosphatase/metabolism ; Animals ; CHO Cells ; Cricetulus ; Enzyme Assays ; Glycoproteins/isolation & purification ; Glycoproteins/metabolism ; Glycosylation ; HEK293 Cells ; Hep G2 Cells ; Humans ; Mutagenesis, Site-Directed ; Recombinant Proteins/genetics ; Recombinant Proteins/isolation & purification ; Recombinant Proteins/metabolism
: Czasopismo naukowe

Szczegóły Pełny tekst

Skocz do pozycji: 11 11.

Tytuł :: Current Practice in Bicistronic IRES Reporter Use: A Systematic Review.
Autorzy :: van den Akker GGH; Department of Orthopedic Surgery, Maastricht University, Medical Center+, 6229 ER Maastricht, The Netherlands.
Zacchini F; Department of Experimental, Diagnostic and Specialty Medicine, Bologna University, I-40138 Bologna, Italy.; Centro di Ricerca Biomedica Applicata-CRBA, Bologna University, Policlinico di Sant'Orsola, I-40138 Bologna, Italy.
Housmans BAC; Department of Orthopedic Surgery, Maastricht University, Medical Center+, 6229 ER Maastricht, The Netherlands.
van der Vloet L; Department of Orthopedic Surgery, Maastricht University, Medical Center+, 6229 ER Maastricht, The Netherlands.
Caron MMJ; Department of Orthopedic Surgery, Maastricht University, Medical Center+, 6229 ER Maastricht, The Netherlands.
Montanaro L; Department of Experimental, Diagnostic and Specialty Medicine, Bologna University, I-40138 Bologna, Italy.; Centro di Ricerca Biomedica Applicata-CRBA, Bologna University, Policlinico di Sant'Orsola, I-40138 Bologna, Italy.; Programma Dipartimentale in Medicina di Laboratorio, IRCCS Azienda Ospedaliero-Universitaria di Bologna, Via Albertoni 15, I-40138 Bologna, Italy.
Welting TJM; Department of Orthopedic Surgery, Maastricht University, Medical Center+, 6229 ER Maastricht, The Netherlands.; Pokaż więcej
Źródło :: International journal of molecular sciences [Int J Mol Sci] 2021 May 14; Vol. 22 (10). Date of Electronic Publication: 2021 May 14.
Typ publikacji :: Journal Article; Systematic Review
MeSH Terms :: Genes, Reporter*
Internal Ribosome Entry Sites*
Protein Biosynthesis*
Regulatory Sequences, Nucleic Acid*
Ribosomes/*metabolism
Animals ; Humans ; Ribosomes/genetics
: Czasopismo naukowe

Szczegóły Pełny tekst

Skocz do pozycji: 12 12.

Tytuł :: Principles and Practical Considerations for the Analysis of Disease-Associated Alternative Splicing Events Using the Gateway Cloning-Based Minigene Vectors pDESTsplice and pSpliceExpress.
Autorzy :: Putscher E; Division of Neuroimmunology, Department of Neurology, Rostock University Medical Center, Gehlsheimer Street 20, 18147 Rostock, Germany.
Hecker M; Division of Neuroimmunology, Department of Neurology, Rostock University Medical Center, Gehlsheimer Street 20, 18147 Rostock, Germany.
Fitzner B; Division of Neuroimmunology, Department of Neurology, Rostock University Medical Center, Gehlsheimer Street 20, 18147 Rostock, Germany.
Lorenz P; Rostock University Medical Center, Institute of Immunology, Schillingallee 70, 18057 Rostock, Germany.
Zettl UK; Division of Neuroimmunology, Department of Neurology, Rostock University Medical Center, Gehlsheimer Street 20, 18147 Rostock, Germany.; Pokaż więcej
Źródło :: International journal of molecular sciences [Int J Mol Sci] 2021 May 13; Vol. 22 (10). Date of Electronic Publication: 2021 May 13.
Typ publikacji :: Journal Article; Review
MeSH Terms :: Alternative Splicing*
Cloning, Molecular*
Genes, Reporter*
Genome, Human*
Mutation*
Genetic Diseases, Inborn/*diagnosis
Genetic Vectors/*genetics
DNA Restriction Enzymes ; Genetic Diseases, Inborn/genetics ; Humans
: Czasopismo naukowe

Szczegóły Pełny tekst

Skocz do pozycji: 13 13.

Tytuł :: Combining transposon mutagenesis and reporter genes to identify novel regulators of the topA promoter in Streptomyces.
Autorzy :: Gongerowska-Jac M; Faculty of Biotechnology, University of Wroclaw, Wroclaw, Poland. .
Szafran MJ; Faculty of Biotechnology, University of Wroclaw, Wroclaw, Poland.
Jakimowicz D; Faculty of Biotechnology, University of Wroclaw, Wroclaw, Poland.; Pokaż więcej
Źródło :: Microbial cell factories [Microb Cell Fact] 2021 May 13; Vol. 20 (1), pp. 99. Date of Electronic Publication: 2021 May 13.
Typ publikacji :: Journal Article
MeSH Terms :: Gene Expression Regulation, Bacterial*
Genes, Reporter*
Promoter Regions, Genetic*
Bacterial Proteins/*genetics
DNA Transposable Elements/*genetics
Mutagenesis, Insertional/*methods
Streptomyces/*genetics
Proof of Concept Study
: Czasopismo naukowe

Szczegóły Pełny tekst

Skocz do pozycji: 14 14.

Tytuł :: Sensitive High-Throughput Assays for Tumour Burden Reveal the Response of a Drosophila melanogaster Model of Colorectal Cancer to Standard Chemotherapies.
Autorzy :: Adams J; Department of Biomedical Science and Bateson Centre, The University of Sheffield, Sheffield S10 2TN, UK.
Casali A; Departament de Ciències Mèdiques Bàsiques, Universitat de Lleida and IRBLleida, 25198 Lleida, Spain.
Campbell K; Department of Biomedical Science and Bateson Centre, The University of Sheffield, Sheffield S10 2TN, UK.; Pokaż więcej
Źródło :: International journal of molecular sciences [Int J Mol Sci] 2021 May 12; Vol. 22 (10). Date of Electronic Publication: 2021 May 12.
Typ publikacji :: Journal Article
MeSH Terms :: Genes, Reporter*
Antineoplastic Agents/*administration & dosage
Colorectal Neoplasms/*drug therapy
Colorectal Neoplasms/*pathology
Animals ; Animals, Genetically Modified ; Antineoplastic Agents/pharmacology ; Colorectal Neoplasms/metabolism ; Drosophila melanogaster ; Drug Screening Assays, Antitumor ; Female ; Fluorouracil/administration & dosage ; Fluorouracil/pharmacology ; Green Fluorescent Proteins/genetics ; Green Fluorescent Proteins/metabolism ; High-Throughput Screening Assays ; Humans ; Luciferases/genetics ; Luciferases/metabolism ; Oxaliplatin/administration & dosage ; Oxaliplatin/pharmacology ; Tumor Burden/drug effects ; Xenograft Model Antitumor Assays
: Czasopismo naukowe

Szczegóły Pełny tekst

Skocz do pozycji: 15 15.

Tytuł :: Establishment of a firefly luciferase reporter assay system in the unicellular red alga Cyanidioschyzon merolae.
Autorzy :: Zhou B; Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology.; School of Life Science and Technology, Tokyo Institute of Technology.
Takahashi S; Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology.; Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology.
Takemura T; Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology.; School of Life Science and Technology, Tokyo Institute of Technology.
Tanaka K; Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology.
Imamura S; Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology.; Pokaż więcej
Źródło :: The Journal of general and applied microbiology [J Gen Appl Microbiol] 2021 Apr 16; Vol. 67 (1), pp. 42-46. Date of Electronic Publication: 2020 Sep 16.
Typ publikacji :: Journal Article
MeSH Terms :: Genes, Reporter*
Luciferases, Firefly/*genetics
Luciferases, Firefly/*metabolism
Rhodophyta/*enzymology
Rhodophyta/*genetics
Gene Expression Regulation, Plant ; Genes, Plant/genetics ; Nitrite Reductases ; Plant Proteins/genetics ; Plant Proteins/metabolism ; Promoter Regions, Genetic ; Transcription Factors/genetics
: Czasopismo naukowe

Szczegóły

Skocz do pozycji: 16 16.

Tytuł :: Strategy for generation of replication-competent recombinant rotaviruses expressing multiple foreign genes.
Autorzy :: Hatazawa R; Department of Molecular Laboratory Medicine, Clinical Laboratory Medicine, Fujita Health University Graduate School of Health Sciences, Toyoake, Aichi 470-1192, Japan.; Department of Virology and Parasitology, Fujita Health University School of Medicine, Toyoake, Aichi 470-1192, Japan.
Fukuda S; Department of Virology and Parasitology, Fujita Health University School of Medicine, Toyoake, Aichi 470-1192, Japan.
Kumamoto K; Education and Research Facility of Animal Models for Human Diseases, Fujita Health University, Toyoake, Aichi 470-1192, Japan.
Matsushita F; Department of Molecular Laboratory Medicine, Clinical Laboratory Medicine, Fujita Health University Graduate School of Health Sciences, Toyoake, Aichi 470-1192, Japan.
Nagao S; Education and Research Facility of Animal Models for Human Diseases, Fujita Health University, Toyoake, Aichi 470-1192, Japan.
Murata T; Department of Virology and Parasitology, Fujita Health University School of Medicine, Toyoake, Aichi 470-1192, Japan.
Taniguchi K; Department of Virology and Parasitology, Fujita Health University School of Medicine, Toyoake, Aichi 470-1192, Japan.
Matsui T; Department of Molecular Laboratory Medicine, Clinical Laboratory Medicine, Fujita Health University Graduate School of Health Sciences, Toyoake, Aichi 470-1192, Japan.
Komoto S; Department of Virology and Parasitology, Fujita Health University School of Medicine, Toyoake, Aichi 470-1192, Japan.; Pokaż więcej
Źródło :: The Journal of general virology [J Gen Virol] 2021 Apr; Vol. 102 (4).
Typ publikacji :: Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :: Genes, Reporter*
Genetic Vectors*
RNA, Viral*
Reverse Genetics*
Rotavirus/*genetics
Animals ; Cell Line ; Cricetinae ; Haplorhini ; Plasmids ; Rotavirus/physiology ; Virus Replication
: Czasopismo naukowe

Szczegóły

Skocz do pozycji: 17 17.

Tytuł :: Cell-Based Reporter Release Assay to Determine the Activity of Calcium-Dependent Neurotoxins and Neuroactive Pharmaceuticals.
Autorzy :: Pathe-Neuschäfer-Rube A; Department of Nutritional Biochemistry, Institute of Nutritional Science, University of Potsdam, 14469 Potsdam, Germany.
Neuschäfer-Rube F; Department of Nutritional Biochemistry, Institute of Nutritional Science, University of Potsdam, 14469 Potsdam, Germany.
Püschel GP; Department of Nutritional Biochemistry, Institute of Nutritional Science, University of Potsdam, 14469 Potsdam, Germany.; Pokaż więcej
Źródło :: Toxins [Toxins (Basel)] 2021 Mar 30; Vol. 13 (4). Date of Electronic Publication: 2021 Mar 30.
Typ publikacji :: Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :: Genes, Reporter*
Calcium/*metabolism
Calcium Channel Blockers/*pharmacology
Calcium Channels/*drug effects
Muscarinic Agonists/*pharmacology
Muscarinic Antagonists/*pharmacology
Neuroblastoma/*metabolism
Neurotoxins/*pharmacology
Secretory Vesicles/*drug effects
Calcium Channels/genetics ; Calcium Channels/metabolism ; Calcium Signaling/drug effects ; Cell Line, Tumor ; Humans ; Luciferases/genetics ; Luciferases/metabolism ; Neuroblastoma/genetics ; Neuroblastoma/pathology ; Pro-Opiomelanocortin/genetics ; Pro-Opiomelanocortin/metabolism ; Secretory Vesicles/genetics ; Secretory Vesicles/metabolism ; Spider Venoms/pharmacology
: Czasopismo naukowe

Szczegóły Pełny tekst

Skocz do pozycji: 18 18.

Tytuł :: A lacZ reporter with high activity in the human fungal pathogen Candida albicans.
Autorzy :: Klimova N; Department of Medicine, McGill University Health Centre, McGill University, Montréal H3A 1A1, Québec, Canada.
Chu S; Department of Medicine, McGill University Health Centre, McGill University, Montréal H3A 1A1, Québec, Canada.
Turcotte B; Department of Medicine, McGill University Health Centre, McGill University, Montréal H3A 1A1, Québec, Canada.; Department of Biochemistry, McGill University Health Centre, McGill University, Montréal H3A 1A1, Québec, Canada.; Department of Microbiology and Immunology, McGill University Health Centre, McGill University, Montréal H3A 1A1, Québec, Canada.; Pokaż więcej
Źródło :: FEMS yeast research [FEMS Yeast Res] 2021 Mar 18; Vol. 21 (2).
Typ publikacji :: Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :: Genes, Reporter*
Candida albicans/*genetics
Lac Operon/*genetics
Animals ; Candida albicans/pathogenicity ; Gene Expression ; Humans ; Mice ; Promoter Regions, Genetic ; Vibrio cholerae/genetics ; beta-Galactosidase/metabolism
: Czasopismo naukowe

Szczegóły

Skocz do pozycji: 19 19.

Tytuł :: High-Throughput Screening for CEBPD-Modulating Compounds in THP-1-Derived Reporter Macrophages Identifies Anti-Inflammatory HDAC and BET Inhibitors.
Autorzy :: Ullmann T; Fraunhofer Institute for Translational Medicine and Pharmacology ITMP, Theodor-Stern-Kai 7, 60596 Frankfurt am Main, Germany.
Luckhardt S; Fraunhofer Institute for Translational Medicine and Pharmacology ITMP, Theodor-Stern-Kai 7, 60596 Frankfurt am Main, Germany.
Wolf M; Fraunhofer Institute for Translational Medicine and Pharmacology ITMP, Schnackenburgallee 114, 22525 Hamburg, Germany.
Parnham MJ; Fraunhofer Institute for Translational Medicine and Pharmacology ITMP, Theodor-Stern-Kai 7, 60596 Frankfurt am Main, Germany.; EpiEndo Pharmaceuticals ehf, Eiðistorg 13-15, 170 Seltjarnarnes, Iceland.
Resch E; Fraunhofer Institute for Translational Medicine and Pharmacology ITMP, Theodor-Stern-Kai 7, 60596 Frankfurt am Main, Germany.; Pokaż więcej
Źródło :: International journal of molecular sciences [Int J Mol Sci] 2021 Mar 16; Vol. 22 (6). Date of Electronic Publication: 2021 Mar 16.
Typ publikacji :: Journal Article
MeSH Terms :: Genes, Reporter*
High-Throughput Screening Assays*
Anti-Inflammatory Agents/*pharmacology
CCAAT-Enhancer-Binding Protein-delta/*metabolism
Histone Deacetylase Inhibitors/*pharmacology
Macrophages/*metabolism
Alkaline Phosphatase/metabolism ; Azepines/pharmacology ; CCAAT-Enhancer-Binding Protein-delta/antagonists & inhibitors ; Gene Expression Regulation/drug effects ; HEK293 Cells ; Heterocyclic Compounds, 4 or More Rings/pharmacology ; Humans ; Hydroxamic Acids/pharmacology ; Luminescent Measurements ; Macrophages/drug effects ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; THP-1 Cells ; Thiophenes/pharmacology ; Vorinostat/pharmacology
: Czasopismo naukowe

Szczegóły

Skocz do pozycji: 20 20.

Tytuł :: Isolating live cell clones from barcoded populations using CRISPRa-inducible reporters.
Autorzy :: Umkehrer C; Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Vienna, Austria.
Holstein F; Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Vienna, Austria.
Formenti L; Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Vienna, Austria.
Jude J; Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Vienna, Austria.
Froussios K; Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Vienna, Austria.
Neumann T; Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Vienna, Austria.
Cronin SM; Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Vienna, Austria.
Haas L; Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Vienna, Austria.
Lipp JJ; Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Vienna, Austria.; Boehringer Ingelheim RCV GmbH & Co. KG, Vienna, Austria.
Burkard TR; Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Vienna, Austria.
Fellner M; Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Vienna, Austria.
Wiesner T; Department of Dermatology, Medical University of Vienna, Vienna, Austria.
Zuber J; Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Vienna, Austria.; Medical University of Vienna, Vienna BioCenter (VBC), Vienna, Austria.
Obenauf AC; Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Vienna, Austria. .; Pokaż więcej
Źródło :: Nature biotechnology [Nat Biotechnol] 2021 Feb; Vol. 39 (2), pp. 174-178. Date of Electronic Publication: 2020 Jul 27.
Typ publikacji :: Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :: Cell Separation*
Genes, Reporter*
CRISPR-Cas Systems/*genetics
Clone Cells/*metabolism
Animals ; Cell Line ; Female ; Humans ; Melanoma/pathology ; Mice, Inbred C57BL ; Mitogen-Activated Protein Kinase Kinases/antagonists & inhibitors ; Protein Kinase Inhibitors/pharmacology ; raf Kinases/antagonists & inhibitors
: Czasopismo naukowe

Szczegóły

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