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Tytuł :
Modelling genetic mosaicism of neurodevelopmental disorders in vivo by a Cre-amplifying fluorescent reporter.
Autorzy :
Trovato F; National Enterprise for Nanoscience and Nanotechnology (NEST), Istituto Nanoscienze Consiglio Nazionale delle Ricerche (CNR) and Scuola Normale Superiore Pisa, 56127, Pisa, Italy. .
Parra R; National Enterprise for Nanoscience and Nanotechnology (NEST), Istituto Nanoscienze Consiglio Nazionale delle Ricerche (CNR) and Scuola Normale Superiore Pisa, 56127, Pisa, Italy.
Pracucci E; National Enterprise for Nanoscience and Nanotechnology (NEST), Istituto Nanoscienze Consiglio Nazionale delle Ricerche (CNR) and Scuola Normale Superiore Pisa, 56127, Pisa, Italy.
Landi S; National Enterprise for Nanoscience and Nanotechnology (NEST), Istituto Nanoscienze Consiglio Nazionale delle Ricerche (CNR) and Scuola Normale Superiore Pisa, 56127, Pisa, Italy.; Institute of Neuroscience CNR, Pisa, Italy.
Cozzolino O; National Enterprise for Nanoscience and Nanotechnology (NEST), Istituto Nanoscienze Consiglio Nazionale delle Ricerche (CNR) and Scuola Normale Superiore Pisa, 56127, Pisa, Italy.
Nardi G; National Enterprise for Nanoscience and Nanotechnology (NEST), Istituto Nanoscienze Consiglio Nazionale delle Ricerche (CNR) and Scuola Normale Superiore Pisa, 56127, Pisa, Italy.
Cruciani F; National Enterprise for Nanoscience and Nanotechnology (NEST), Istituto Nanoscienze Consiglio Nazionale delle Ricerche (CNR) and Scuola Normale Superiore Pisa, 56127, Pisa, Italy.
Pillai V; National Enterprise for Nanoscience and Nanotechnology (NEST), Istituto Nanoscienze Consiglio Nazionale delle Ricerche (CNR) and Scuola Normale Superiore Pisa, 56127, Pisa, Italy.
Mosti L; National Enterprise for Nanoscience and Nanotechnology (NEST), Istituto Nanoscienze Consiglio Nazionale delle Ricerche (CNR) and Scuola Normale Superiore Pisa, 56127, Pisa, Italy.
Cwetsch AW; Istituto Italiano di Tecnologia, Genoa, Italy.; Università degli studi di Genova, Genoa, Italy.
Cancedda L; Istituto Italiano di Tecnologia, Genoa, Italy.; Istituto Telethon Dulbecco, Rome, Italy.
Gritti L; Institute of Neuroscience CNR, Milan, Italy.
Sala C; Institute of Neuroscience CNR, Milan, Italy.
Verpelli C; Institute of Neuroscience CNR, Milan, Italy.
Maset A; Veneto Institute of Molecular Medicine, Padua, Italy.; Padova Neuroscience Center, Padova Università di Padova, Padua, Italy.
Lodovichi C; Veneto Institute of Molecular Medicine, Padua, Italy.; Padova Neuroscience Center, Padova Università di Padova, Padua, Italy.; Institute of Neuroscience CNR, Padua, Italy.
Ratto GM; National Enterprise for Nanoscience and Nanotechnology (NEST), Istituto Nanoscienze Consiglio Nazionale delle Ricerche (CNR) and Scuola Normale Superiore Pisa, 56127, Pisa, Italy. .
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Źródło :
Nature communications [Nat Commun] 2020 Dec 03; Vol. 11 (1), pp. 6194. Date of Electronic Publication: 2020 Dec 03.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Genes, Reporter*
Mosaicism*
Fluorescent Dyes/*chemistry
Integrases/*metabolism
Neurodevelopmental Disorders/*genetics
Action Potentials ; Animals ; Animals, Newborn ; Disease Models, Animal ; Electroencephalography ; Gene Expression ; HEK293 Cells ; Humans ; Mice ; Mice, Inbred C57BL ; NIH 3T3 Cells ; Neurodevelopmental Disorders/physiopathology ; PTEN Phosphohydrolase/metabolism ; Tamoxifen/pharmacology
Czasopismo naukowe
Tytuł :
A robust CRISPR-Cas9-based fluorescent reporter assay for the detection and quantification of DNA double-strand break repair.
Autorzy :
Eki R; Department of Radiation Oncology, University of Virginia, Charlottesville, VA 22908, USA.; Department of Biochemistry and Molecular Genetics, University of Virginia, Charlottesville, VA 22908, USA.; Center for Cell Signaling, University of Virginia, Charlottesville, VA 22908, USA.
She J; Department of Radiation Oncology, University of Virginia, Charlottesville, VA 22908, USA.
Parlak M; Department of Radiation Oncology, University of Virginia, Charlottesville, VA 22908, USA.
Benamar M; Department of Radiation Oncology, University of Virginia, Charlottesville, VA 22908, USA.; Department of Biochemistry and Molecular Genetics, University of Virginia, Charlottesville, VA 22908, USA.; Center for Cell Signaling, University of Virginia, Charlottesville, VA 22908, USA.
Du KP; Department of Radiation Oncology, University of Virginia, Charlottesville, VA 22908, USA.
Kumar P; Department of Biochemistry and Molecular Genetics, University of Virginia, Charlottesville, VA 22908, USA.
Abbas T; Department of Radiation Oncology, University of Virginia, Charlottesville, VA 22908, USA.; Department of Biochemistry and Molecular Genetics, University of Virginia, Charlottesville, VA 22908, USA.; Center for Cell Signaling, University of Virginia, Charlottesville, VA 22908, USA.; Cancer Center, University of Virginia, Charlottesville, VA 22908, USA.
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Źródło :
Nucleic acids research [Nucleic Acids Res] 2020 Dec 02; Vol. 48 (21), pp. e126.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural
MeSH Terms :
DNA Breaks, Double-Stranded*
DNA Repair*
Genes, Reporter*
Biological Assay/*methods
CRISPR-Cas Systems/*genetics
Fluorescent Dyes/*chemistry
Ataxia Telangiectasia Mutated Proteins/metabolism ; Cell Line, Tumor ; DNA Ligase ATP/metabolism ; DNA Repair Enzymes/metabolism ; DNA-Binding Proteins/metabolism ; HEK293 Cells ; Humans
Czasopismo naukowe
Tytuł :
An expanded palette of improved SPLICS reporters detects multiple organelle contacts in vitro and in vivo.
Autorzy :
Vallese F; Department of Biomedical Sciences, University of Padova, Padova, Italy.
Catoni C; Department of Biology, University of Padova, Padova, Italy.
Cieri D; Department of Biomedical Sciences, University of Padova, Padova, Italy.
Barazzuol L; Department of Biomedical Sciences, University of Padova, Padova, Italy.
Ramirez O; Department of Neurobiology, Interdisciplinary Center for Neurosciences, Heidelberg University, Heidelberg, Germany.
Calore V; Department of Biomedical Sciences, University of Padova, Padova, Italy.
Bonora M; Department of Morphology, Surgery and Experimental Medicine, Section of General Pathology, University of Ferrara, Ferrara, Italy.; Laboratory for Technologies of Advanced Therapies (LTTA), University of Ferrara, Ferrara, Italy.
Giamogante F; Department of Biomedical Sciences, University of Padova, Padova, Italy.
Pinton P; Department of Morphology, Surgery and Experimental Medicine, Section of General Pathology, University of Ferrara, Ferrara, Italy.; Laboratory for Technologies of Advanced Therapies (LTTA), University of Ferrara, Ferrara, Italy.
Brini M; Department of Biology, University of Padova, Padova, Italy. .
Calì T; Department of Biomedical Sciences, University of Padova, Padova, Italy. .; Padova Neuroscience Center (PNC), University of Padova, Padova, Italy. .
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Źródło :
Nature communications [Nat Commun] 2020 Nov 27; Vol. 11 (1), pp. 6069. Date of Electronic Publication: 2020 Nov 27.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Genes, Reporter*
Green Fluorescent Proteins/*metabolism
Organelles/*metabolism
Animals ; Calcium/metabolism ; Cell Membrane/metabolism ; Cytosol/metabolism ; Endoplasmic Reticulum/metabolism ; HeLa Cells ; Humans ; Neurons/metabolism ; Rats, Sprague-Dawley ; Zebrafish/metabolism
Czasopismo naukowe
Tytuł :
[Constraction of triple negative breast cancer cell model containing EGFR promoter and luciferase reporter gene].
Autorzy :
Liang D; Department of Pharmaceutical Analysis, Guangxi Medical University, Nanning 530021, China.
Wang C; Department of Pharmaceutical Analysis, Guangxi Medical University, Nanning 530021, China.
Li J; Department of Pharmaceutical Analysis, Guangxi Medical University, Nanning 530021, China.
Qin L; Department of Pharmaceutical Analysis, Guangxi Medical University, Nanning 530021, China.
Su Z; Department of Pharmacy, Guangxi International Zhuang Medicine Hospital, Nanning 530201, China.
Hou H; Department of Pharmaceutical Analysis, Guangxi Medical University, Nanning 530021, China. *Corresponding author, E-mail: .
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Źródło :
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology [Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi] 2020 Oct; Vol. 36 (10), pp. 918-923.
Typ publikacji :
Journal Article
MeSH Terms :
Genes, Reporter*
Promoter Regions, Genetic*
Triple Negative Breast Neoplasms/*genetics
Cell Line, Tumor ; ErbB Receptors/genetics ; Humans ; Luciferases/genetics
Czasopismo naukowe
Tytuł :
Development of Strong Anaerobic Fluorescent Reporters for Clostridium acetobutylicum and Clostridium ljungdahlii Using HaloTag and SNAP-tag Proteins.
Autorzy :
Charubin K; Department of Chemical and Biomolecular Engineering, University of Delaware, Newark, Delaware, USA.; Delaware Biotechnology Institute, University of Delaware, Newark, Delaware, USA.
Streett H; Delaware Biotechnology Institute, University of Delaware, Newark, Delaware, USA.; Department of Biological Sciences, University of Delaware, Newark, Delaware, USA.
Papoutsakis ET; Department of Chemical and Biomolecular Engineering, University of Delaware, Newark, Delaware, USA .; Delaware Biotechnology Institute, University of Delaware, Newark, Delaware, USA.; Department of Biological Sciences, University of Delaware, Newark, Delaware, USA.
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Źródło :
Applied and environmental microbiology [Appl Environ Microbiol] 2020 Oct 01; Vol. 86 (20). Date of Electronic Publication: 2020 Oct 01 (Print Publication: 2020).
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
MeSH Terms :
Genes, Bacterial*
Genes, Reporter*
Bacterial Proteins/*genetics
Bacteriological Techniques/*methods
Clostridium/*genetics
Clostridium acetobutylicum/*genetics
Absorption, Physiological ; Anaerobiosis ; Fluorescence ; Promoter Regions, Genetic
SCR Organism :
Clostridium ljungdahlii
Czasopismo naukowe
Tytuł :
A multiplexed bioluminescent reporter for sensitive and non-invasive tracking of DNA double strand break repair dynamics in vitro and in vivo.
Autorzy :
Chien JC; Institute of Atomic and Molecular Sciences, Academia Sinica, Taipei 10617, Taiwan.
Tabet E; Department of Neurology, Massachusetts General Hospital, Boston, MA 02129, USA.; Department of Biomedical Engineering, University of South Dakota, 4800 N. Career Ave, Suite 221, Sioux Falls, Vermillion, SD 57069, USA.
Pinkham K; Department of Neurology, Massachusetts General Hospital, Boston, MA 02129, USA.
da Hora CC; Department of Neurology, Massachusetts General Hospital, Boston, MA 02129, USA.; Neuroscience Program, Harvard Medical School, Boston, MA 02115, USA.
Chang JC; Institute of Biochemical Sciences, National Taiwan University, Taipei 10617, Taiwan.; Institute of Biological Chemistry, Academia Sinica, Taipei 115, Taiwan.
Lin S; Institute of Biochemical Sciences, National Taiwan University, Taipei 10617, Taiwan.; Institute of Biological Chemistry, Academia Sinica, Taipei 115, Taiwan.
Badr CE; Department of Neurology, Massachusetts General Hospital, Boston, MA 02129, USA.; Neuroscience Program, Harvard Medical School, Boston, MA 02115, USA.
Lai CP; Institute of Atomic and Molecular Sciences, Academia Sinica, Taipei 10617, Taiwan.; Chemical Biology and Molecular Biophysics Program, Taiwan International Graduate Program, Academia Sinica, Taipei 115, Taiwan.; Genome and Systems Biology Degree Program, National Taiwan University and Academia Sinica, Taipei 115, Taiwan.
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Źródło :
Nucleic acids research [Nucleic Acids Res] 2020 Sep 25; Vol. 48 (17), pp. e100.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
MeSH Terms :
Genes, Reporter*
Recombinational DNA Repair*
Luciferases/*genetics
Animals ; CRISPR-Cas Systems ; Cell Line, Tumor ; Copepoda/enzymology ; DNA End-Joining Repair ; Female ; HEK293 Cells ; High-Throughput Nucleotide Sequencing/methods ; Humans ; Luciferases/metabolism ; Mice ; Mice, Nude ; Multiplex Polymerase Chain Reaction/methods ; Optical Imaging/methods ; Rad51 Recombinase/genetics ; Rad51 Recombinase/metabolism ; Sequence Analysis, DNA/methods
Czasopismo naukowe
Tytuł :
Establishment of a stable transfection method in Babesia microti and identification of a novel bidirectional promoter of Babesia microti.
Autorzy :
Jaijyan DK; National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi, 10067, India.
Govindasamy K; MJKS Research LLC, Edison, NJ, USA. .
Singh J; National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi, 10067, India.
Bhattacharya S; National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi, 10067, India.
Singh AP; National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi, 10067, India. .
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Źródło :
Scientific reports [Sci Rep] 2020 Sep 24; Vol. 10 (1), pp. 15614. Date of Electronic Publication: 2020 Sep 24.
Typ publikacji :
Journal Article
MeSH Terms :
Genes, Reporter*
Promoter Regions, Genetic*
Babesia microti/*genetics
Babesia microti/*growth & development
Babesiosis/*parasitology
Genetic Vectors/*administration & dosage
Transfection/*standards
Animals ; Babesiosis/pathology ; Genetic Vectors/genetics ; Mice ; Mice, Inbred C57BL ; Transfection/methods
Czasopismo naukowe
Tytuł :
A New Class of Dengue and West Nile Virus Protease Inhibitors with Submicromolar Activity in Reporter Gene DENV-2 Protease and Viral Replication Assays.
Autorzy :
Kühl N; Medicinal Chemistry, Institute of Pharmacy and Molecular Biotechnology IPMB, Heidelberg University, Im Neuenheimer Feld 364, 69120 Heidelberg, Germany.
Graf D; Medicinal Chemistry, Institute of Pharmacy and Molecular Biotechnology IPMB, Heidelberg University, Im Neuenheimer Feld 364, 69120 Heidelberg, Germany.
Bock J; Medicinal Chemistry, Institute of Pharmacy and Molecular Biotechnology IPMB, Heidelberg University, Im Neuenheimer Feld 364, 69120 Heidelberg, Germany.
Behnam MAM; Medicinal Chemistry, Institute of Pharmacy and Molecular Biotechnology IPMB, Heidelberg University, Im Neuenheimer Feld 364, 69120 Heidelberg, Germany.
Leuthold MM; Medicinal Chemistry, Institute of Pharmacy and Molecular Biotechnology IPMB, Heidelberg University, Im Neuenheimer Feld 364, 69120 Heidelberg, Germany.
Klein CD; Medicinal Chemistry, Institute of Pharmacy and Molecular Biotechnology IPMB, Heidelberg University, Im Neuenheimer Feld 364, 69120 Heidelberg, Germany.
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Źródło :
Journal of medicinal chemistry [J Med Chem] 2020 Aug 13; Vol. 63 (15), pp. 8179-8197. Date of Electronic Publication: 2020 Jul 15.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Dengue Virus/*drug effects
Genes, Reporter/*drug effects
Protease Inhibitors/*administration & dosage
Protease Inhibitors/*chemistry
Virus Replication/*drug effects
West Nile virus/*drug effects
Animals ; Chlorocebus aethiops ; Dengue Virus/physiology ; Dose-Response Relationship, Drug ; Genes, Reporter/physiology ; HeLa Cells ; Humans ; Vero Cells ; Virus Replication/physiology ; West Nile virus/physiology
Czasopismo naukowe
Tytuł :
Systematized reporter assays reveal ZIC protein regulatory abilities are Subclass-specific and dependent upon transcription factor binding site context.
Autorzy :
Ahmed JN; Early Mammalian Development Laboratory, John Curtin School of Medical Research, The Australian National University, Canberra, ACT, 2601, Australia.
Diamand KEM; Early Mammalian Development Laboratory, John Curtin School of Medical Research, The Australian National University, Canberra, ACT, 2601, Australia.
Bellchambers HM; Early Mammalian Development Laboratory, John Curtin School of Medical Research, The Australian National University, Canberra, ACT, 2601, Australia.; Department of Pediatrics, Indiana University School of Medicine, Indianapolis, IN, USA.
Arkell RM; Early Mammalian Development Laboratory, John Curtin School of Medical Research, The Australian National University, Canberra, ACT, 2601, Australia. .
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Źródło :
Scientific reports [Sci Rep] 2020 Aug 04; Vol. 10 (1), pp. 13130. Date of Electronic Publication: 2020 Aug 04.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Gene Expression Regulation*
Genes, Reporter*
Response Elements*
Transcription Factors*/genetics
Transcription Factors*/metabolism
Transcription, Genetic*
HEK293 Cells ; Humans
Czasopismo naukowe
Tytuł :
Forward Genetic Screen Using Transgenic Calcium Reporter Aequorin to Identify Novel Targets in Calcium Signaling.
Autorzy :
Mittal D; National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi 110067, India.
Mishra S; National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi 110067, India.
Prajapati R; National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi 110067, India.
Vadassery J; National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi 110067, India; .
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Źródło :
Journal of visualized experiments : JoVE [J Vis Exp] 2020 Aug 01 (162). Date of Electronic Publication: 2020 Aug 01.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't; Video-Audio Media
MeSH Terms :
Calcium Signaling*/drug effects
Genes, Reporter*
Genetic Testing*
Transgenes*
Aequorin/*genetics
Calcium/*metabolism
Aequorin/metabolism ; Arabidopsis/drug effects ; Arabidopsis/genetics ; Arabidopsis/metabolism ; Hydrogen Peroxide/toxicity ; Mutagenesis/genetics ; Mutation/genetics ; Phenotype ; Plants, Genetically Modified ; Seeds/drug effects ; Seeds/genetics ; Seeds/metabolism
Czasopismo naukowe
Tytuł :
Genetically encoded betaxanthin-based small-molecular fluorescent reporter for mammalian cells.
Autorzy :
Stücheli P; Department of Biosystems Science and Engineering, ETH Zurich, Mattenstrasse 26, CH-4058 Basel, Switzerland.
Sieber S; Department of Chemistry, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland.
Fuchs DW; Department of Biosystems Science and Engineering, ETH Zurich, Mattenstrasse 26, CH-4058 Basel, Switzerland.
Scheller L; Department of Biosystems Science and Engineering, ETH Zurich, Mattenstrasse 26, CH-4058 Basel, Switzerland.
Strittmatter T; Department of Biosystems Science and Engineering, ETH Zurich, Mattenstrasse 26, CH-4058 Basel, Switzerland.
Saxena P; Department of Biosystems Science and Engineering, ETH Zurich, Mattenstrasse 26, CH-4058 Basel, Switzerland.
Gademann K; Department of Chemistry, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland.
Fussenegger M; Department of Biosystems Science and Engineering, ETH Zurich, Mattenstrasse 26, CH-4058 Basel, Switzerland.; Faculty of Science, University of Basel, Mattenstrasse 26, CH-4058 Basel, Switzerland.
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Źródło :
Nucleic acids research [Nucleic Acids Res] 2020 Jul 09; Vol. 48 (12), pp. e67.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Genes, Reporter*
Fungal Proteins/*metabolism
Oxygenases/*metabolism
Picolinic Acids/*metabolism
Single-Cell Analysis/*methods
Absorption, Radiation ; Animals ; CHO Cells ; Cricetinae ; Cricetulus ; Fungal Proteins/genetics ; HEK293 Cells ; Humans ; Microscopy, Fluorescence/methods ; Oxygenases/genetics ; Picolinic Acids/radiation effects ; Recombinant Proteins/genetics ; Recombinant Proteins/metabolism ; Spectrometry, Fluorescence/methods ; Tyrosine 3-Monooxygenase/genetics ; Tyrosine 3-Monooxygenase/metabolism ; Ultraviolet Rays
Czasopismo naukowe
Tytuł :
A reporter cell line for real-time imaging of autophagy and apoptosis.
Autorzy :
Lupitha SS; Cancer Research Program, Rajiv Gandhi Centre for Biotechnology (Research Centre, University of Kerala), Poojappura, Thycaud P.O., Thiruvananthapuram, Kerala, 695014, India.
Chandrasekhar L; Cancer Research Program, Rajiv Gandhi Centre for Biotechnology (Research Centre, University of Kerala), Poojappura, Thycaud P.O., Thiruvananthapuram, Kerala, 695014, India.
Varadarajan SN; Cancer Research Program, Rajiv Gandhi Centre for Biotechnology (Research Centre, University of Kerala), Poojappura, Thycaud P.O., Thiruvananthapuram, Kerala, 695014, India.
Jayaprasad AG; Cancer Research Program, Rajiv Gandhi Centre for Biotechnology (Research Centre, University of Kerala), Poojappura, Thycaud P.O., Thiruvananthapuram, Kerala, 695014, India.
Chandrasekharan A; Cancer Research Program, Rajiv Gandhi Centre for Biotechnology (Research Centre, University of Kerala), Poojappura, Thycaud P.O., Thiruvananthapuram, Kerala, 695014, India.
Patil SN; Cancer Research Program, Rajiv Gandhi Centre for Biotechnology (Research Centre, University of Kerala), Poojappura, Thycaud P.O., Thiruvananthapuram, Kerala, 695014, India.
Mini M; Cancer Research Program, Rajiv Gandhi Centre for Biotechnology (Research Centre, University of Kerala), Poojappura, Thycaud P.O., Thiruvananthapuram, Kerala, 695014, India.
Pillai PR; Cancer Research Program, Rajiv Gandhi Centre for Biotechnology (Research Centre, University of Kerala), Poojappura, Thycaud P.O., Thiruvananthapuram, Kerala, 695014, India.
Santhoshkumar TR; Cancer Research Program, Rajiv Gandhi Centre for Biotechnology (Research Centre, University of Kerala), Poojappura, Thycaud P.O., Thiruvananthapuram, Kerala, 695014, India. Electronic address: .
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Źródło :
Toxicology letters [Toxicol Lett] 2020 Jun 15; Vol. 326, pp. 23-30. Date of Electronic Publication: 2020 Feb 25.
Typ publikacji :
Journal Article
MeSH Terms :
Apoptosis/*drug effects
Autophagy/*drug effects
Cell Line, Tumor/*drug effects
Drug Evaluation, Preclinical/*methods
Genes, Reporter/*drug effects
Green Fluorescent Proteins/*drug effects
HeLa Cells/*drug effects
Apoptosis/physiology ; Autophagy/physiology ; Cell Line, Tumor/physiology ; Genes, Reporter/physiology ; Green Fluorescent Proteins/physiology ; HeLa Cells/physiology ; Humans
Czasopismo naukowe
Tytuł :
A gene signal amplifier platform for monitoring the unfolded protein response.
Autorzy :
Origel Marmolejo CA; BioScience Department, Rice University, Houston, TX, USA.
Bachhav B; Chemical and Biomolecular Engineering Department, Rice University, Houston, TX, USA.
Patibandla SD; BioScience Department, Rice University, Houston, TX, USA.
Yang AL; Department of Computational and Applied Mathematics, Rice University, Houston, TX, USA.
Segatori L; BioScience Department, Rice University, Houston, TX, USA. .; Chemical and Biomolecular Engineering Department, Rice University, Houston, TX, USA. .; Bioengineering Department, Rice University, Houston, TX, USA. .
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Źródło :
Nature chemical biology [Nat Chem Biol] 2020 May; Vol. 16 (5), pp. 520-528. Date of Electronic Publication: 2020 Mar 09.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
MeSH Terms :
Genes, Reporter*
Gene Expression Profiling/*methods
Unfolded Protein Response/*genetics
Activating Transcription Factor 6/genetics ; Chromosomes/genetics ; Computational Biology/methods ; Endoplasmic Reticulum ; Endoribonucleases/genetics ; Green Fluorescent Proteins/genetics ; Green Fluorescent Proteins/metabolism ; HEK293 Cells ; Humans ; Protein Biosynthesis ; Protein-Serine-Threonine Kinases/genetics ; Transcription, Genetic ; eIF-2 Kinase/genetics
Czasopismo naukowe
Tytuł :
Characterization of a nap1l1 transgenic reporter in zebrafish.
Autorzy :
Sun S; Children's Hospital, Fudan University, Shanghai 201102, PR China; Shanghai Municipal Eighth People's Hospital, Shanghai 200235, PR China.
Liu Z; Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital and Institute of Biomedical Sciences, Fudan University, Shanghai 200032, PR China.
Li X; Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital and Institute of Biomedical Sciences, Fudan University, Shanghai 200032, PR China.
Jia J; Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital and Institute of Biomedical Sciences, Fudan University, Shanghai 200032, PR China.
Zhang G; Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital and Institute of Biomedical Sciences, Fudan University, Shanghai 200032, PR China.
Yang C; Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital and Institute of Biomedical Sciences, Fudan University, Shanghai 200032, PR China.
Jiang Q; Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital and Institute of Biomedical Sciences, Fudan University, Shanghai 200032, PR China. Electronic address: .
Zou Y; Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital and Institute of Biomedical Sciences, Fudan University, Shanghai 200032, PR China. Electronic address: .
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Źródło :
Gene [Gene] 2020 Apr 20; Vol. 735, pp. 144388. Date of Electronic Publication: 2020 Jan 24.
Typ publikacji :
Journal Article
MeSH Terms :
Genes, Reporter*
Transgenes*
Lateral Line System/*metabolism
Nucleosome Assembly Protein 1/*genetics
Zebrafish/*genetics
Zebrafish Proteins/*genetics
Animals ; Green Fluorescent Proteins/genetics ; Green Fluorescent Proteins/metabolism ; Lateral Line System/growth & development ; Nucleosome Assembly Protein 1/metabolism ; Promoter Regions, Genetic ; Zebrafish/growth & development ; Zebrafish Proteins/metabolism
Czasopismo naukowe
Tytuł :
Simplified Reverse Genetics Method to Recover Recombinant Rotaviruses Expressing Reporter Proteins.
Autorzy :
Philip AA; Department of Biology, Indiana University.
Dai J; Department of Biology, Indiana University.
Katen SP; Department of Biology, Indiana University.
Patton JT; Department of Biology, Indiana University; .
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Źródło :
Journal of visualized experiments : JoVE [J Vis Exp] 2020 Apr 17 (158). Date of Electronic Publication: 2020 Apr 17.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Video-Audio Media
MeSH Terms :
Genes, Reporter*
Recombination, Genetic/*genetics
Reverse Genetics/*methods
Rotavirus/*genetics
Viral Proteins/*metabolism
Animals ; Cell Line ; Cell Survival ; DNA-Directed RNA Polymerases/metabolism ; Plasmids/genetics ; Plasmids/metabolism ; RNA, Viral/genetics ; Sequence Analysis, RNA
Czasopismo naukowe
Tytuł :
GO: a functional reporter system to identify and enrich base editing activity.
Autorzy :
Katti A; Sandra and Edward Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USA.; Graduate School of Medical Sciences, Weill Cornell Medicine, New York, NY 10065, USA.
Foronda M; Sandra and Edward Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USA.
Zimmerman J; Sandra and Edward Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USA.; Graduate School of Medical Sciences, Weill Cornell Medicine, New York, NY 10065, USA.
Diaz B; Sandra and Edward Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USA.; Graduate School of Medical Sciences, Weill Cornell Medicine, New York, NY 10065, USA.
Zafra MP; Sandra and Edward Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USA.
Goswami S; Sandra and Edward Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USA.
Dow LE; Sandra and Edward Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USA.; Department of Medicine, Weill Cornell Medicine, New York, NY 10065, USA.; Department of Biochemistry, Weill Cornell Medicine, New York, NY 10021, USA.
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Źródło :
Nucleic acids research [Nucleic Acids Res] 2020 Apr 06; Vol. 48 (6), pp. 2841-2852.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural
MeSH Terms :
Gene Editing*
Genes, Reporter*
Animals ; Base Sequence ; Cell Line, Tumor ; Drug Resistance, Microbial ; HEK293 Cells ; Humans ; Integrases/metabolism ; Mice ; NIH 3T3 Cells ; Recombination, Genetic/genetics
Czasopismo naukowe
Tytuł :
A Novel Reporter Gene Assay for Pyrogen Detection.
Autorzy :
He Q; National Institutes for Food and Drug Control.
Yu C; National Institutes for Food and Drug Control.
Wang L; National Institutes for Food and Drug Control.
Ni Y; National Institutes for Food and Drug Control.
Zhang H; National Institutes for Food and Drug Control.
Du Y; National Institutes for Food and Drug Control.
Gao H; National Institutes for Food and Drug Control.
Wang J; National Institutes for Food and Drug Control.
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Źródło :
Japanese journal of infectious diseases [Jpn J Infect Dis] 2020 Mar 24; Vol. 73 (2), pp. 111-118. Date of Electronic Publication: 2019 Oct 31.
Typ publikacji :
Journal Article
MeSH Terms :
Genes, Reporter*
NF-kappa B/*genetics
Pyrogens/*analysis
Animals ; Biological Assay/methods ; Fever/diagnosis ; Fever/etiology ; Limit of Detection ; Mice ; Pyrogens/chemistry ; RAW 264.7 Cells ; Sensitivity and Specificity
Czasopismo naukowe
Tytuł :
Generation and validation of a PITX2-EGFP reporter line of human induced pluripotent stem cells enables isolation of periocular mesenchymal cells.
Autorzy :
Okubo T; Department of Stem Cells and Applied Medicine, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan.; Basic Research Development Division, Rohto Pharmaceutical Co., Ltd., Osaka 544-8666, Japan.
Hayashi R; Department of Stem Cells and Applied Medicine, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan .; Department of Ophthalmology, Graduate School of Medicine, Osaka University, Suita, Osaka 565-0871, Japan.
Shibata S; Department of Stem Cells and Applied Medicine, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan.; Basic Research Development Division, Rohto Pharmaceutical Co., Ltd., Osaka 544-8666, Japan.
Kudo Y; Department of Stem Cells and Applied Medicine, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan.; Basic Research Development Division, Rohto Pharmaceutical Co., Ltd., Osaka 544-8666, Japan.
Ishikawa Y; Department of Ophthalmology, Graduate School of Medicine, Osaka University, Suita, Osaka 565-0871, Japan.
Inoue S; Department of Ophthalmology, Graduate School of Medicine, Osaka University, Suita, Osaka 565-0871, Japan.
Kobayashi Y; Department of Ophthalmology, Graduate School of Medicine, Osaka University, Suita, Osaka 565-0871, Japan.
Honda A; Department of Ophthalmology, Graduate School of Medicine, Osaka University, Suita, Osaka 565-0871, Japan.
Honma Y; Department of Stem Cells and Applied Medicine, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan.; Basic Research Development Division, Rohto Pharmaceutical Co., Ltd., Osaka 544-8666, Japan.
Kawasaki S; Department of Ophthalmology, Graduate School of Medicine, Osaka University, Suita, Osaka 565-0871, Japan.
Nishida K; Department of Ophthalmology, Graduate School of Medicine, Osaka University, Suita, Osaka 565-0871, Japan.; Integrated Frontier Research for Medical Science Division, Institute for Open and Transdisciplinary Research Initiatives, Osaka University, Suita, Osaka 565-0871, Japan.
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Źródło :
The Journal of biological chemistry [J Biol Chem] 2020 Mar 13; Vol. 295 (11), pp. 3456-3465. Date of Electronic Publication: 2020 Feb 07.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't; Validation Study
MeSH Terms :
Cell Separation*
Genes, Reporter*
Eye/*cytology
Green Fluorescent Proteins/*metabolism
Homeodomain Proteins/*metabolism
Induced Pluripotent Stem Cells/*cytology
Mesenchymal Stem Cells/*metabolism
Transcription Factors/*metabolism
Animals ; Cell Line ; Clone Cells ; Ectoderm/cytology ; Embryo, Mammalian/cytology ; Fluorescence ; Humans ; Mice, Inbred ICR ; Phenotype ; Promoter Regions, Genetic/genetics ; RNA Splicing/genetics ; Reproducibility of Results
Czasopismo naukowe
Tytuł :
The mito -QC Reporter for Quantitative Mitophagy Assessment in Primary Retinal Ganglion Cells and Experimental Glaucoma Models.
Autorzy :
Rosignol I; Department of Cellular and Molecular Biology, Centro de Investigaciones Biológicas Margarita Salas, CSIC, 28040 Madrid, Spain.
Villarejo-Zori B; Department of Cellular and Molecular Biology, Centro de Investigaciones Biológicas Margarita Salas, CSIC, 28040 Madrid, Spain.
Teresak P; Department of Cellular and Molecular Biology, Centro de Investigaciones Biológicas Margarita Salas, CSIC, 28040 Madrid, Spain.
Sierra-Filardi E; Department of Cellular and Molecular Biology, Centro de Investigaciones Biológicas Margarita Salas, CSIC, 28040 Madrid, Spain.
Pereiro X; Experimental-Ophthalmo-Biology Group, Universidad del País Vasco, 48950 Leioa, Spain.
Rodríguez-Muela N; Department of Cellular and Molecular Biology, Centro de Investigaciones Biológicas Margarita Salas, CSIC, 28040 Madrid, Spain.
Vecino E; Experimental-Ophthalmo-Biology Group, Universidad del País Vasco, 48950 Leioa, Spain.
Vieira HLA; UCIBIO, REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, 2829-516 Caparica, Portugal.; CEDOC, NOVA Medical School/Faculdade de Ciências Médicas, Universidade Nova de Lisboa, 1150-082 Lisbon, Portugal.
Bell K; Department of Cellular and Molecular Biology, Centro de Investigaciones Biológicas Margarita Salas, CSIC, 28040 Madrid, Spain.
Boya P; Department of Cellular and Molecular Biology, Centro de Investigaciones Biológicas Margarita Salas, CSIC, 28040 Madrid, Spain.
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Źródło :
International journal of molecular sciences [Int J Mol Sci] 2020 Mar 10; Vol. 21 (5). Date of Electronic Publication: 2020 Mar 10.
Typ publikacji :
Journal Article
MeSH Terms :
Genes, Reporter*
Deferiprone/*pharmacology
Glaucoma/*metabolism
Iron Chelating Agents/*pharmacology
Mitochondria/*metabolism
Retinal Ganglion Cells/*cytology
Animals ; Cell Survival/drug effects ; Cells, Cultured ; Disease Models, Animal ; Glaucoma/etiology ; Humans ; Mice ; Mitophagy ; Primary Cell Culture ; Rats ; Retinal Ganglion Cells/drug effects ; Retinal Ganglion Cells/metabolism
Czasopismo naukowe
Tytuł :
A Novel Fluorescent Reporter System Identifies Laminin-511/521 as Potent Regulators of Cardiomyocyte Maturation.
Autorzy :
Chanthra N; Division of Regenerative Medicine, Center for Molecular Medicine, Jichi Medical University, Shimotsuke, Japan.
Abe T; Division of Regenerative Medicine, Center for Molecular Medicine, Jichi Medical University, Shimotsuke, Japan.; Division of Stem Cell Research and Drug Development, Center for Development of Advanced Medical Technology, Jichi Medical University, Shimotsuke, Japan.
Miyamoto M; Division of Cardiology, Johns Hopkins University School of Medicine, Baltimore, USA.; Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, USA.
Sekiguchi K; Division of Matrixome Research and Application, Institute for Protein Research, Osaka University, Osaka, Japan.
Kwon C; Division of Cardiology, Johns Hopkins University School of Medicine, Baltimore, USA.; Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, USA.
Hanazono Y; Division of Regenerative Medicine, Center for Molecular Medicine, Jichi Medical University, Shimotsuke, Japan.; Division of Stem Cell Research and Drug Development, Center for Development of Advanced Medical Technology, Jichi Medical University, Shimotsuke, Japan.
Uosaki H; Division of Regenerative Medicine, Center for Molecular Medicine, Jichi Medical University, Shimotsuke, Japan. .; Division of Stem Cell Research and Drug Development, Center for Development of Advanced Medical Technology, Jichi Medical University, Shimotsuke, Japan. .
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Źródło :
Scientific reports [Sci Rep] 2020 Mar 06; Vol. 10 (1), pp. 4249. Date of Electronic Publication: 2020 Mar 06.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Biomarkers*
Cell Differentiation*/genetics
Gene Expression*
Genes, Reporter*
Laminin/*genetics
Myocytes, Cardiac/*cytology
Myocytes, Cardiac/*metabolism
Calcium ; Computational Biology/methods ; Extracellular Matrix/metabolism ; Gene Expression Profiling ; Laminin/metabolism ; Pluripotent Stem Cells/cytology ; Pluripotent Stem Cells/metabolism ; Sarcomeres/metabolism ; Transcription, Genetic ; Transcriptome
Czasopismo naukowe

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