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Tytuł :
Evaluation of the diagnostic performance of an immunoblot for ANCA and anti-GBM antibody detection.
Autorzy :
Busch MH; Department of Nephrology and Clinical Immunology, Maastricht University Medical Center, Maastricht, The Netherlands.
Aendekerk JP; Department of Nephrology and Clinical Immunology, Maastricht University Medical Center, Maastricht, The Netherlands.
van Beers JJBC; Central Diagnostic Laboratory, Maastricht University Medical Center, Maastricht, The Netherlands.
van Paassen P; Department of Nephrology and Clinical Immunology, Maastricht University Medical Center, Maastricht, The Netherlands.
Damoiseaux JGMC; Central Diagnostic Laboratory, Maastricht University Medical Center, Maastricht, The Netherlands.
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Źródło :
Autoimmunity [Autoimmunity] 2021 Feb; Vol. 54 (1), pp. 45-50. Date of Electronic Publication: 2020 Nov 20.
Typ publikacji :
Journal Article
MeSH Terms :
Antibodies, Antineutrophil Cytoplasmic/*blood
Autoantibodies/*blood
Immunoblotting/*methods
Antibodies, Antineutrophil Cytoplasmic/immunology ; Autoantibodies/immunology ; Autoimmune Diseases/blood ; Autoimmune Diseases/diagnosis ; Biomarkers ; Biopsy ; Disease Susceptibility/immunology ; Glomerulonephritis/blood ; Glomerulonephritis/diagnosis ; Glomerulonephritis/etiology ; Humans ; Immunoblotting/standards ; Reference Values ; Reproducibility of Results ; Sensitivity and Specificity
Czasopismo naukowe
Tytuł :
R-Loop Analysis by Dot-Blot.
Autorzy :
Ramirez P; Life Sciences Institute, University of Michigan, Ann Arbor.
Crouch RJ; Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health.
Cheung VG; Life Sciences Institute, University of Michigan, Ann Arbor; Department of Pediatrics, University of Michigan, Ann Arbor; Howard Hughes Medical Institute.
Grunseich C; Neurogenetics Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health; .
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Źródło :
Journal of visualized experiments : JoVE [J Vis Exp] 2021 Jan 22 (167). Date of Electronic Publication: 2021 Jan 22.
Typ publikacji :
Journal Article; Research Support, N.I.H., Intramural; Research Support, Non-U.S. Gov't; Video-Audio Media
MeSH Terms :
Immunoblotting*
R-Loop Structures*/genetics
Antibodies/metabolism ; DNA/isolation & purification ; Fibroblasts/metabolism ; Humans ; Nucleic Acid Heteroduplexes/metabolism ; Oligonucleotides/metabolism ; RNA/genetics ; Ribonuclease H/metabolism ; Ribonucleases/metabolism
Czasopismo naukowe
Tytuł :
Immunoblot Validation of Phospho-Specific Antibodies Using Lung Cancer Cell Lines.
Autorzy :
Pedreira-García WM; Biochemistry and Cancer Biology Divisions, Ponce Health Sciences University-Ponce Research Institute, Ponce, Puerto Rico.
Pérez-Morales J; Biochemistry and Cancer Biology Divisions, Ponce Health Sciences University-Ponce Research Institute, Ponce, Puerto Rico.
Chardón-Colón C; Biochemistry and Cancer Biology Divisions, Ponce Health Sciences University-Ponce Research Institute, Ponce, Puerto Rico.
Cabán-Rivera J; Epidemiology Program, School of Public Health, Ponce Health Sciences University-Ponce Research Institute, Ponce, Puerto Rico.
Santiago-Cardona PG; Biochemistry and Cancer Biology Divisions, Ponce Health Sciences University-Ponce Research Institute, Ponce, Puerto Rico. .
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Źródło :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2279, pp. 75-90.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Validation Study
MeSH Terms :
Immunoblotting*
Antibodies, Neoplasm/*chemistry
Antibodies, Phospho-Specific/*chemistry
Lung Neoplasms/*metabolism
Phosphoproteins/*metabolism
Retinoblastoma Protein/*metabolism
Cell Line, Tumor ; Humans ; Lung Neoplasms/pathology
Czasopismo naukowe
Tytuł :
Rapid Detection of Femtogram Amounts of Protein by Gel-Free Immunoblot.
Autorzy :
Shlyapnikov YM; Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow region, Russia. .
Malakhova EA; Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow region, Russia.
Shlyapnikova EA; Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow region, Russia.
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Źródło :
Bulletin of experimental biology and medicine [Bull Exp Biol Med] 2020 Oct; Vol. 169 (6), pp. 840-843. Date of Electronic Publication: 2020 Oct 24.
Typ publikacji :
Journal Article
MeSH Terms :
Electrophoresis/*methods
Immobilized Proteins/*analysis
Immunoblotting/*methods
Immunoglobulin A/*analysis
Immunomagnetic Separation/*methods
Air/analysis ; Azides/chemistry ; Biotin/chemistry ; Biotinylation ; Cellulose/chemistry ; Electrophoresis/instrumentation ; Exhalation/physiology ; Humans ; Immunoblotting/instrumentation ; Limit of Detection ; Membranes, Artificial ; Photochemical Processes ; Serum Albumin, Bovine/chemistry ; Streptavidin/chemistry
Czasopismo naukowe
Tytuł :
Allergenicity assessment of the edible cricket Acheta domesticus in terms of thermal and gastrointestinal processing and IgE cross-reactivity with shrimp.
Autorzy :
De Marchi L; University of Verona, Department of Biotechnology, Verona, Italy.
Mainente F; University of Verona, Department of Biotechnology, Verona, Italy.
Leonardi M; University of Verona, Department of Biotechnology, Verona, Italy.
Scheurer S; Paul-Ehrlich-Institut, Molecular Allergology, Langen, Germany.
Wangorsch A; Paul-Ehrlich-Institut, Molecular Allergology, Langen, Germany.
Mahler V; Paul-Ehrlich-Institut, Division of Allergology, Langen, Germany; Allergy Unit, Dept. of Dermatology, University Hospital Erlangen, Medical Faculty, Friedrich-Alexander-University Erlangen-Nuremberg, Erlangen, Germany.
Pilolli R; Institute of Sciences of Food Production, CNR-ISPA, 70126 Bari, Italy.
Sorio D; University of Verona, Department of Biotechnology, Verona, Italy.
Zoccatelli G; University of Verona, Department of Biotechnology, Verona, Italy. Electronic address: .
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Źródło :
Food chemistry [Food Chem] 2021 Oct 15; Vol. 359, pp. 129878. Date of Electronic Publication: 2021 Apr 20.
Typ publikacji :
Journal Article
MeSH Terms :
Food Hypersensitivity*
Allergens/*analysis
Gryllidae/*immunology
Immunoglobulin E/*analysis
Penaeidae/*chemistry
Shellfish/*analysis
Animals ; Calcium-Binding Proteins/immunology ; Cross Reactions ; Digestion ; Enzyme-Linked Immunosorbent Assay ; Food Handling ; Gryllidae/chemistry ; Humans ; Immunoblotting ; Tropomyosin/immunology
Czasopismo naukowe
Tytuł :
Catalytic Clusterbody for Enhanced Quantitative Protein Immunoblot.
Autorzy :
Li J; Department of Chemistry and Biology, Faculty of Environment and Life Science, Beijing University of Technology, Beijing 100124, China.
Zhang X; Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China.
Gao F; CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China.
Yuan Q; Department of Chemistry and Biology, Faculty of Environment and Life Science, Beijing University of Technology, Beijing 100124, China.
Zhang C; Department of Chemistry and Biology, Faculty of Environment and Life Science, Beijing University of Technology, Beijing 100124, China.
Yuan H; CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China.
Liu Y; Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
Chen L; Department of Chemistry and Biology, Faculty of Environment and Life Science, Beijing University of Technology, Beijing 100124, China.
Han Y; Department of Chemistry and Biology, Faculty of Environment and Life Science, Beijing University of Technology, Beijing 100124, China.
Gao X; Department of Chemistry and Biology, Faculty of Environment and Life Science, Beijing University of Technology, Beijing 100124, China.
Gao L; Department of Chemistry and Biology, Faculty of Environment and Life Science, Beijing University of Technology, Beijing 100124, China.
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Źródło :
Analytical chemistry [Anal Chem] 2021 Aug 10; Vol. 93 (31), pp. 10807-10815. Date of Electronic Publication: 2021 Jul 30.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Biosensing Techniques*
Enzyme-Linked Immunosorbent Assay ; Immunoassay ; Immunoblotting ; Reproducibility of Results
Czasopismo naukowe
Tytuł :
Preparation of Affinity Purified Antibodies against ε-Glutaryl-Lysine Residues in Proteins for Investigation of Glutarylated Proteins in Animal Tissues.
Autorzy :
Artiukhov AV; Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, 119991 Moscow, Russia.; Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119991 Moscow, Russia.
Kolesanova EF; Laboratory of Peptide Engineering, Institute of Biomedical Chemistry, 10/8, Pogodinskaya ul., 119121 Moscow, Russia.
Boyko AI; Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, 119991 Moscow, Russia.
Chashnikova AA; Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, 119991 Moscow, Russia.
Gnedoy SN; 'Agrobiomed' Ltd., 249010 Borovsk, Russia.
Kaehne T; Institute of Experimental Internal Medicine, Otto-von-Guericke University, 39120 Magdeburg, Germany.
Ivanova DA; Laboratory of Peptide Engineering, Institute of Biomedical Chemistry, 10/8, Pogodinskaya ul., 119121 Moscow, Russia.
Kolesnichenko AV; Laboratory of Peptide Engineering, Institute of Biomedical Chemistry, 10/8, Pogodinskaya ul., 119121 Moscow, Russia.
Aleshin VA; Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, 119991 Moscow, Russia.; Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119991 Moscow, Russia.
Bunik VI; Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, 119991 Moscow, Russia.; Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119991 Moscow, Russia.; Department of Biological Chemistry, Sechenov First Moscow State Medical University, 119146 Moscow, Russia.
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Źródło :
Biomolecules [Biomolecules] 2021 Aug 07; Vol. 11 (8). Date of Electronic Publication: 2021 Aug 07.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Protein Processing, Post-Translational*
Glutarates/*metabolism
Lysine/*metabolism
Proteins/*metabolism
Succinates/*metabolism
Acetylation ; Amino Acid Sequence ; Animals ; Antibodies/chemistry ; Antibodies/isolation & purification ; Antibody Specificity ; Brain/metabolism ; Chromatography, Affinity ; Immune Sera/chemistry ; Immunoblotting ; Liver/metabolism ; Male ; Rabbits ; Rats
Czasopismo naukowe
Tytuł :
Combining multi-antigenic immunodot with indirect immunofluorescence on HEp-2 cells improves the diagnosis of systemic sclerosis.
Autorzy :
Bost C; Immunology laboratory, Institut Fédératif de Biologie, Toulouse University Hospital Center, France; INSERM U1043, CNRS UMR 5282, Toulouse III University, Center for Pathophysiology Toulouse Purpan, Toulouse, France. Electronic address: .
Fortenfant F; Immunology laboratory, Institut Fédératif de Biologie, Toulouse University Hospital Center, France. Electronic address: .
Blancher A; Immunology laboratory, Institut Fédératif de Biologie, Toulouse University Hospital Center, France; INSERM U1043, CNRS UMR 5282, Toulouse III University, Center for Pathophysiology Toulouse Purpan, Toulouse, France. Electronic address: .
Pugnet G; Department of Internal Medicine, Toulouse University Hospital Center, France; Clinical Investigation Center 1436, CHU Toulouse, Toulouse, France. Electronic address: .
Renaudineau Y; Immunology laboratory, Institut Fédératif de Biologie, Toulouse University Hospital Center, France; INSERM U1043, CNRS UMR 5282, Toulouse III University, Center for Pathophysiology Toulouse Purpan, Toulouse, France. Electronic address: .
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Źródło :
Clinical immunology (Orlando, Fla.) [Clin Immunol] 2021 Aug; Vol. 229, pp. 108774. Date of Electronic Publication: 2021 Jun 07.
Typ publikacji :
Evaluation Study; Journal Article
MeSH Terms :
Autoantibodies/*blood
Fluorescent Antibody Technique, Indirect/*methods
Immunoblotting/*methods
Scleroderma, Systemic/*diagnosis
Scleroderma, Systemic/*immunology
Adult ; Aged ; Autoantigens/immunology ; Cell Line ; Centromere Protein A/immunology ; Centromere Protein B/immunology ; DNA Topoisomerases, Type I/immunology ; Female ; Humans ; Male ; Middle Aged ; Retrospective Studies
Czasopismo naukowe
Tytuł :
Klebsiella pneumoniae enolase-like membrane protein interacts with human plasminogen.
Autorzy :
Serek P; Department of Biochemistry and Immunochemistry, Wroclaw Medical University, Chałubińskiego 10, 50-368, Wroclaw, Poland.
Lewandowski Ł; Department of Biochemistry and Immunochemistry, Wroclaw Medical University, Chałubińskiego 10, 50-368, Wroclaw, Poland.
Dudek B; Department of Microbiology, Institute of Genetics and Microbiology, University of Wrocław, Przybyszewskiego 63-77, 51-148, Wroclaw, Poland.
Pietkiewicz J; Department of Biochemistry and Immunochemistry, Wroclaw Medical University, Chałubińskiego 10, 50-368, Wroclaw, Poland.
Jermakow K; Department of Microbiology, Faculty of Medicine, Wroclaw Medical University, Chałubińskiego 4, 50-368, Wrocław, Poland.
Kapczyńska K; Department of Immunology of Infectious Diseases, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53-114, Wrocław, Poland.
Krzyżewska E; Department of Immunology of Infectious Diseases, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53-114, Wrocław, Poland.
Bednarz-Misa I; Department of Biochemistry and Immunochemistry, Wroclaw Medical University, Chałubińskiego 10, 50-368, Wroclaw, Poland. Electronic address: .
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Źródło :
International journal of medical microbiology : IJMM [Int J Med Microbiol] 2021 Aug; Vol. 311 (6), pp. 151518. Date of Electronic Publication: 2021 Jul 03.
Typ publikacji :
Journal Article
MeSH Terms :
Klebsiella pneumoniae*
Plasminogen*
Bacterial Outer Membrane Proteins ; Humans ; Immunoblotting ; Phosphopyruvate Hydratase
Czasopismo naukowe
Tytuł :
A new diagnostic algorithm for pattern-oriented autoantibody testing according to the ICAP nomenclature: A pilot study.
Autorzy :
Infantino M; Laboratorio Immunologia Allergologia, Ospedale San Giovanni di Dio, Firenze, Italy. Electronic address: .
Carbone T; IReL, Istituto Reumatologico Lucano, Ospedale San Carlo, Potenza, Italy.
Manfredi M; Laboratorio Immunologia Allergologia, Ospedale San Giovanni di Dio, Firenze, Italy.
Grossi V; Laboratorio Immunologia Allergologia, Ospedale San Giovanni di Dio, Firenze, Italy.
Antico A; Laboratorio Analisi ULSS, Santorso (VI), Italy.
Panozzo MP; Laboratorio Analisi ULSS, Santorso (VI), Italy.
Brusca I; Patologia Clinica, Ospedale Buccheri La Ferla FBF, Palermo, Italy.
Alessio MG; Laboratorio Analisi Chimico Cliniche, ASST Papa Giovanni XXIII, Bergamo, Italy.
Previtali G; Laboratorio Analisi Chimico Cliniche, ASST Papa Giovanni XXIII, Bergamo, Italy.
Platzgummer S; Laboratorio Centrale, Ospedale Civile, Merano, Italy.
Cinquanta L; Laboratorio centralizzato (HUB) SDN Spa, Gruppo SYNLAB, Pagani, SA, Italy.
Paura G; Autoimmmunologia e allergologia diagnostica di laboratorio, OORR San Giovanni di Dio e Ruggi d'Aragona, Salerno, Italy.
Deleonardi G; Laboratorio Unico Metropolitano, Ospedale Maggiore, Bologna, Italy.
Trevisan MT; UOA di Laboratorio, Ospedale G. Fracastoro, Verona, Italy.
Radice A; UOC Microbiologia e Virologia, Presidio Ospedaliero San Carlo Borromeo, Milano, Italy.
Castiglione C; Laboratorio di Immunologia, Ospedale Spirito Santo, Pescara, Italy.
Imbastaro T; Laboratorio di Immunologia, Ospedale Spirito Santo, Pescara, Italy.
Fabris M; SOC Istituto di Patologia Clinica, Azienda Sanitaria Universitaria Integrata, Udine, Italy.
Pesce G; Laboratorio Diagnostico di Autoimmunologia IRCCS Ospedale Policlinico San Martino Genova Dipartimento di Medicina Interna e specialità mediche (DIMI), Università di Genova, Genova, Italy.
Porcelli B; UOC Laboratorio Patologia Clinica, Policlinico S. Maria alle Scotte, AOU Senese, Siena, Italy.
Terzuoli L; UOC Laboratorio Patologia Clinica, Policlinico S. Maria alle Scotte, AOU Senese, Siena, Italy.
Sorrentino MC; Dipartimento di Medicina di Laboratorio e Biotecnologie avanzate, Laboratorio di Patologia Clinica, Microbiologia e Virologia, Palermo, Italy.
Tampoia M; Laboratorio di Autoimmunologia, UOC di Patologia Clinica Universitaria, Bari, Italy.
Abbracciavento L; Laboratorio di Autoimmunologia, UOC di Patologia Clinica Universitaria, Bari, Italy.
Villalta D; SSD di Allergologia e Immunologia clinica, Presidio Ospedaliero S. Maria degli Angeli, Pordenone, Italy.
Conte M; SSD di Allergologia e Immunologia clinica, Presidio Ospedaliero S. Maria degli Angeli, Pordenone, Italy.
Barberio G; U.O.C. Medicina di Laboratorio Azienda ULSS n2 Marca trevigiana, Treviso, Italy.
Gallo N; Dipartimento di Medicina di Laboratorio, Azienda Universitaria di Padova, Padova, Italy.
Benucci M; SOS Reumatologia, Ospedale San Giovanni di Dio, Firenze, Italy.
Bizzaro N; Laboratorio di Patologia Clinica, Ospedale San Antonio, Tolmezzo, Italy.
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Corporate Authors :
Study Group on Autoimmune Diseases of the Italian Society of Clinical Pathology and Laboratory Medicine
Źródło :
Autoimmunity reviews [Autoimmun Rev] 2020 Aug; Vol. 19 (8), pp. 102588. Date of Electronic Publication: 2020 Jun 12.
Typ publikacji :
Journal Article; Review
MeSH Terms :
Algorithms*
Antibodies, Antinuclear*/blood
Autoimmune Diseases*/blood
Autoimmune Diseases*/diagnosis
Diagnostic Techniques and Procedures*
Immunoblotting*/standards
Fluorescent Antibody Technique, Indirect ; Humans ; Pilot Projects
Czasopismo naukowe

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