Ketonization of Ginsenoside C-K by Novel Recombinant 3-β-Hydroxysteroid Dehydrogenases and Effect on Human Fibroblast Cells.
Autorzy:
Jin Y; School of Life Science, Nantong University, Nantong 226019, China. Wang D; College of Life Sciences, Yantai University, Yantai 264005, China. ImWT; Department of Biotechnology, Hankyong National University, 327 Jungang-ro, Anseong-si 17579, Gyeonggi-do, Republic of Korea.; Department of Oriental Medicinal Material & Processing, College of Life Science, Kyung Hee University, Seocheon-dong, Giheung-gu, Yongin-si 17104, Gyeonggi-do, Republic of Korea. Siddiqi MZ; Department of Biotechnology, Hankyong National University, 327 Jungang-ro, Anseong-si 17579, Gyeonggi-do, Republic of Korea. Yang DC; Department of Oriental Medicinal Material & Processing, College of Life Science, Kyung Hee University, Seocheon-dong, Giheung-gu, Yongin-si 17104, Gyeonggi-do, Republic of Korea.
Production of Prosaikogenin F, Prosaikogenin G, Saikogenin F and Saikogenin G by the Recombinant Enzymatic Hydrolysis of Saikosaponin and their Anti-Cancer Effect.
Autorzy:
Lee JE; Department of Biotechnology, Major in Applied Biotechnology, Hankyong National University, 327 Chungang-no, Anseong-si 17579, Gyeonggi-do, Korea. Song BK; AceEMzyme Co., Ltd., Academic Industry Cooperation, 327 Chungang-no, Anseong-si 17579, Gyeonggi-do, Korea. Kim JH; AceEMzyme Co., Ltd., Academic Industry Cooperation, 327 Chungang-no, Anseong-si 17579, Gyeonggi-do, Korea. Siddiqi MZ; Department of Biotechnology, Major in Applied Biotechnology, Hankyong National University, 327 Chungang-no, Anseong-si 17579, Gyeonggi-do, Korea. ImWT; Department of Biotechnology, Major in Applied Biotechnology, Hankyong National University, 327 Chungang-no, Anseong-si 17579, Gyeonggi-do, Korea.; AceEMzyme Co., Ltd., Academic Industry Cooperation, 327 Chungang-no, Anseong-si 17579, Gyeonggi-do, Korea.; HK Ginseng Research Center, 327 Chungang-no, Anseong-si 17579, Gyeonggi-do, Korea.
Exploration and Characterization of Novel Glycoside Hydrolases from the Whole Genome of Lactobacillus ginsenosidimutans and Enriched Production of Minor Ginsenoside Rg3( S ) by a Recombinant Enzymatic Process.
Autorzy:
Siddiqi MZ; Department of Biotechnology, Hankyong National University, 327 Jungang-ro Anseong-si, Gyeonggi-do 17579, Korea.; AceEMzyme Co., Ltd., Academic Industry Cooperation, 327 Jungang-ro Anseong-si, Gyeonggi-do Anseong-si, Gyeonggi-do 17579, Korea. Srinivasan S; Department of Bio & Environmental Technology, Division of Environmental & Life Science, College of Natural Science, Seoul Women's University, 623 Hwarangno, Nowon-gu, Seoul 139-774, Korea. Park HY; National Institute of Biological Resources, Incheon 22689, Korea. ImWT; Department of Biotechnology, Hankyong National University, 327 Jungang-ro Anseong-si, Gyeonggi-do 17579, Korea.; AceEMzyme Co., Ltd., Academic Industry Cooperation, 327 Jungang-ro Anseong-si, Gyeonggi-do Anseong-si, Gyeonggi-do 17579, Korea.
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Źródło:
Biomolecules [Biomolecules] 2020 Feb 12; Vol. 10 (2). Date of Electronic Publication: 2020 Feb 12.
High-density immobilization of a ginsenoside-transforming β-glucosidase for enhanced food-grade production of minor ginsenosides.
Autorzy:
Cui CH; Intelligent Synthetic Biology Center, 291 Daehak-Ro, Yuseong-Gu, Daejeon, 305-701, Korea.; The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, Jiangsu Normal University, No. 101 Shanghai Road, Xuzhou, Jiangsu, 221116, People's Republic of China. Jeon BM; Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 291 Daehak-Ro, Yuseong-Gu, Daejeon, 305-701, Korea. Fu Y; The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, Jiangsu Normal University, No. 101 Shanghai Road, Xuzhou, Jiangsu, 221116, People's Republic of China. ImWT; Department of Biological Sciences, Hankyong National University, 327 Chungang-Ro, Anseong City, Kyonggi-Do, 456-749, Korea. Kim SC; Intelligent Synthetic Biology Center, 291 Daehak-Ro, Yuseong-Gu, Daejeon, 305-701, Korea. .; Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 291 Daehak-Ro, Yuseong-Gu, Daejeon, 305-701, Korea. .; KAIST Institute for Biocentury, Korea Advanced Institute of Science and Technology, 291 Daehak-Ro, Yuseong-Gu, Daejeon, 305-701, Korea. .
Identification of novel glycoside hydrolases via whole genome sequencing of Niabella ginsenosidivorans for production of various minor ginsenosides.
Autorzy:
Siddiqi MZ; 1Department of Biotechnology, Hankyong National University, 327 Jungang-ro, Anseong-si, Gyeonggi-do 17579 Republic of Korea.; AceEMzyme Co., Ltd., Room 403, Academic Industry Cooperation, 327 Jungang-ro, Anseong-si, Gyeonggi-do 17579 Republic of Korea. Hashmi MS; 3Department of Food Science and Technology Faculty of Nutrition Sciences, The University of Agriculture, Peshawar, Khyber Pakhtunkhwa 25120 Pakistan. Oh JM; 4Department of Physiology, Medical School, Chonbuk National University, Geonji-ro 20, Jeonju, Jeonbuk 54907 Republic of Korea. Chun S; 4Department of Physiology, Medical School, Chonbuk National University, Geonji-ro 20, Jeonju, Jeonbuk 54907 Republic of Korea. ImWT; 1Department of Biotechnology, Hankyong National University, 327 Jungang-ro, Anseong-si, Gyeonggi-do 17579 Republic of Korea.; AceEMzyme Co., Ltd., Room 403, Academic Industry Cooperation, 327 Jungang-ro, Anseong-si, Gyeonggi-do 17579 Republic of Korea.
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Źródło:
3 Biotech [3 Biotech] 2019 Jul; Vol. 9 (7), pp. 258. Date of Electronic Publication: 2019 Jun 10.
Ginsenoside Rk1 Induces Apoptosis in Neuroblastoma Cells Through Loss of Mitochondrial Membrane Potential and Activation of Caspases.
Autorzy:
Oh JM; Department of Physiology, Chonbuk National University Medical School, Jeonju 54907, Korea. . Lee J; Department of Anesthesiology and Pain Medicine, Chonbuk National University Hospital, Jeonju 54907, Korea. . ImWT; Department of Biotechnology, Hankyoung National University, Anseong 17579, Korea. . Chun S; Department of Physiology, Chonbuk National University Medical School, Jeonju 54907, Korea. .; Research Institute of Clinical Medicine of Chonbuk National University, Jeonju 54907, Korea. .; Biomedical Research Institute of Chonbuk National University Hospital, Jeonju 54907, Korea. .
Biotic elicitation of ginsenoside metabolism of mutant adventitious root culture in Panax ginseng.
Autorzy:
Le KC; Department of Horticultural Science, Division of Animal, Horticulture and Food Sciences, Chungbuk National University, Cheongju, 28644, Republic of Korea. ImWT; Department of Biotechnology, Hankyong National University, Anseong, 17579, Republic of Korea. Paek KY; Department of Horticultural Science, Division of Animal, Horticulture and Food Sciences, Chungbuk National University, Cheongju, 28644, Republic of Korea. Park SY; Department of Horticultural Science, Division of Animal, Horticulture and Food Sciences, Chungbuk National University, Cheongju, 28644, Republic of Korea. .
Enhanced Production of Gypenoside LXXV Using a Novel Ginsenoside-Transforming β-Glucosidase from Ginseng-Cultivating Soil Bacteria and Its Anti-Cancer Property.
Autorzy:
Cui CH; Intelligent Synthetic Biology Center, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Korea. . Kim DJ; Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Korea. . Jung SC; Intelligent Synthetic Biology Center, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Korea. . Kim SC; Intelligent Synthetic Biology Center, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Korea. .; Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Korea. .; KAIST Institute for Biocentury, Korea Advanced Institute of Science and Technology, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Korea. . ImWT; Department of Biological Sciences, Hankyong National University, 327 Chungang-no Anseong-si, Kyonggi-do 456-749, Korea. .
Comparative analysis of the expression level of recombinant ginsenoside-transforming β-glucosidase in GRAS hosts and mass production of the ginsenoside Rh2-Mix.
Autorzy:
Siddiqi MZ; Department of Biotechnology, Hankyoung National University, Kyonggi-do, Republic of Korea.; Center for Genetic Information, Graduate School of Bio and Information Technology, Hankyoung National University, Kyonggi-do, Republic of Korea. Cui CH; Intelligent Synthetic Biology Center, Yuseong-gu, Daejeon, Republic of Korea. Park SK; Intelligent Synthetic Biology Center, Yuseong-gu, Daejeon, Republic of Korea. Han NS; Brain Korea 21 Center for Bio-Resource Development, Division of Animal, Horticultural and Food Sciences, Chungbuk National University, Cheongju, Korea. Kim SC; Intelligent Synthetic Biology Center, Yuseong-gu, Daejeon, Republic of Korea. ImWT; Department of Biotechnology, Hankyoung National University, Kyonggi-do, Republic of Korea.; Center for Genetic Information, Graduate School of Bio and Information Technology, Hankyoung National University, Kyonggi-do, Republic of Korea.
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Źródło:
PloS one [PLoS One] 2017 Apr 19; Vol. 12 (4), pp. e0176098. Date of Electronic Publication: 2017 Apr 19 (Print Publication: 2017).
Two ginseng UDP-glycosyltransferases synthesize ginsenoside Rg3 and Rd.
Autorzy:
Jung SC; Department of Biological Sciences, KAIST, Daejeon 305-701, Republic of Korea. Kim W; Department of Biological Sciences, KAIST, Daejeon 305-701, Republic of Korea. Park SC; Department of Biological Sciences, KAIST, Daejeon 305-701, Republic of Korea. Jeong J; Department of Biological Sciences, KAIST, Daejeon 305-701, Republic of Korea. Park MK; Department of Biological Sciences, KAIST, Daejeon 305-701, Republic of Korea. Lim S; Department of Biological Sciences, KAIST, Daejeon 305-701, Republic of Korea. Lee Y; Department of Biological Sciences, KAIST, Daejeon 305-701, Republic of Korea. ImWT; KAIST Institute for Biocentury, KAIST, Daejeon, 305-701, Republic of Korea. Lee JH; KAIST Institute for Biocentury, KAIST, Daejeon, 305-701, Republic of Korea. Choi G; Department of Biological Sciences, KAIST, Daejeon 305-701, Republic of Korea . Kim SC; Department of Biological Sciences, KAIST, Daejeon 305-701, Republic of Korea .
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Źródło:
Plant & cell physiology [Plant Cell Physiol] 2014 Dec; Vol. 55 (12), pp. 2177-88. Date of Electronic Publication: 2014 Oct 14.
The first complete genome sequence of the class Fimbriimonadia in the phylum Armatimonadetes.
Autorzy:
Hu ZY; Department of Microbiology and Microbial Engineering, School of Life Sciences, Fudan University, Shanghai, China. Wang YZ; Shanghai-MOST Key Laboratory of Health and Disease Genomics, Chinese National Human Genome Center at Shanghai, Shanghai, China. ImWT; Department of Biotechnology, Hankyoung National Univeristy, Kyonggi-do, Republic of Korea. Wang SY; Shanghai-MOST Key Laboratory of Health and Disease Genomics, Chinese National Human Genome Center at Shanghai, Shanghai, China. Zhao GP; Department of Microbiology and Microbial Engineering, School of Life Sciences, Fudan University, Shanghai, China; Shanghai-MOST Key Laboratory of Health and Disease Genomics, Chinese National Human Genome Center at Shanghai, Shanghai, China. Zheng HJ; Shanghai-MOST Key Laboratory of Health and Disease Genomics, Chinese National Human Genome Center at Shanghai, Shanghai, China; Laboratory of Medical Foods, Shanghai Institute of Planned Parenthood Research, Shanghai, China. Quan ZX; Department of Microbiology and Microbial Engineering, School of Life Sciences, Fudan University, Shanghai, China.
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Źródło:
PloS one [PLoS One] 2014 Jun 26; Vol. 9 (6), pp. e100794. Date of Electronic Publication: 2014 Jun 26 (Print Publication: 2014).
Identification and characterization of a ginsenoside-transforming β-glucosidase from Pseudonocardia sp. Gsoil 1536 and its application for enhanced production of minor ginsenoside Rg2(S).
Autorzy:
Du J; KAIST Institute for Biocentury, Korea Advanced Institute of Science and Technology, Yuseong-gu, Daejeon, Republic of Korea; College of Biotechnology, Dalian Polytechnic University, Ganjingzi-qu, Dalian, P. R. China. Cui CH; Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Yuseong-gu, Daejeon, Republic of Korea. Park SC; Intelligent Synthetic Biology Center, Yuseong-gu, Daejeon, Republic of Korea. Kim JK; KAIST Institute for Biocentury, Korea Advanced Institute of Science and Technology, Yuseong-gu, Daejeon, Republic of Korea. Yu HS; College of Biotechnology, Dalian Polytechnic University, Ganjingzi-qu, Dalian, P. R. China. Jin FX; College of Biotechnology, Dalian Polytechnic University, Ganjingzi-qu, Dalian, P. R. China. Sun C; Institute for Brain Disorders, Dalian Medical University, Dalian, P.R. China. Kim SC; KAIST Institute for Biocentury, Korea Advanced Institute of Science and Technology, Yuseong-gu, Daejeon, Republic of Korea; Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Yuseong-gu, Daejeon, Republic of Korea; Intelligent Synthetic Biology Center, Yuseong-gu, Daejeon, Republic of Korea. ImWT; Department of Biotechnology, Hankyoung National University, Chungang-no Anseong-si, Republic of Korea.
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Źródło:
PloS one [PLoS One] 2014 Jun 09; Vol. 9 (6), pp. e96914. Date of Electronic Publication: 2014 Jun 09 (Print Publication: 2014).
Characterization of a ginsenoside-transforming β-glucosidase from Paenibacillus mucilaginosus and its application for enhanced production of minor ginsenoside F₂.
Autorzy:
Cui CH; Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Yuseong-gu, Daejeon, Republic of Korea. Kim JK; KAIST Institute for Biocentury, Korea Advanced Institute of Science and Technology, Yuseong-gu, Daejeon, Republic of Korea. Kim SC; Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Yuseong-gu, Daejeon, Republic of Korea ; KAIST Institute for Biocentury, Korea Advanced Institute of Science and Technology, Yuseong-gu, Daejeon, Republic of Korea ; Intelligent Synthetic Biology Center, Yuseong-gu, Daejeon, Republic of Korea. ImWT; KAIST Institute for Biocentury, Korea Advanced Institute of Science and Technology, Yuseong-gu, Daejeon, Republic of Korea.
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Źródło:
PloS one [PLoS One] 2014 Jan 27; Vol. 9 (1), pp. e85727. Date of Electronic Publication: 2014 Jan 27 (Print Publication: 2014).
Characterization of the ginsenoside-transforming recombinant β-glucosidase from Actinosynnema mirum and bioconversion of major ginsenosides into minor ginsenosides.
Autorzy:
Cui CH; Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea. Kim SC ImWT
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