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Wyszukujesz frazę ""Ligase chain reaction"" wg kryterium: Temat


Tytuł:
Integrated microRNA and transcriptome profiling reveal key miRNA-mRNA interaction pairs associated with seed development in Tartary buckwheat (Fagopyrum tataricum).
Autorzy:
Li H; Research Center of Buckwheat Industry Technology, Guizhou Normal University, Guiyang, 550001, China. .
Meng H; Key Laboratory of High-Quality Crops Cultivation and Safety Control of Yunnan Province, Honghe University, Honghe, 661100, China.
Sun X; Research Center of Buckwheat Industry Technology, Guizhou Normal University, Guiyang, 550001, China.; School of Life Sciences, Guizhou Normal University, Guiyang, 550025, China.
Deng J; Research Center of Buckwheat Industry Technology, Guizhou Normal University, Guiyang, 550001, China.
Shi T; Research Center of Buckwheat Industry Technology, Guizhou Normal University, Guiyang, 550001, China.
Zhu L; Research Center of Buckwheat Industry Technology, Guizhou Normal University, Guiyang, 550001, China.
Lv Q; School of Big Data and Computer Science, Guizhou Normal University, Guiyang, 550025, China.
Chen Q; Research Center of Buckwheat Industry Technology, Guizhou Normal University, Guiyang, 550001, China. .
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Źródło:
BMC plant biology [BMC Plant Biol] 2021 Mar 09; Vol. 21 (1), pp. 132. Date of Electronic Publication: 2021 Mar 09.
Typ publikacji:
Journal Article; Validation Study
MeSH Terms:
Fagopyrum/*genetics
Fagopyrum/*growth & development
MicroRNAs/*physiology
RNA, Messenger/*physiology
RNA, Plant/*physiology
Seeds/*growth & development
Evolution, Molecular ; Gene Expression Profiling ; Ligase Chain Reaction ; MicroRNAs/genetics ; Phylogeny ; Plant Development/genetics ; RNA, Plant/genetics ; Real-Time Polymerase Chain Reaction ; Seeds/genetics
Czasopismo naukowe
Tytuł:
Integration of magnetic separation and real-time ligation chain reaction for detection of uracil-DNA glycosylase.
Autorzy:
Liu J; Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Key Laboratory of Analytical Science and Technology of Hebei Province, College of Chemistry and Environmental Science, Institute of Life Science and Green Development, Hebei University, Baoding, 071002, China.
Zhang J; Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Key Laboratory of Analytical Science and Technology of Hebei Province, College of Chemistry and Environmental Science, Institute of Life Science and Green Development, Hebei University, Baoding, 071002, China. .
Chen M; Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Key Laboratory of Analytical Science and Technology of Hebei Province, College of Chemistry and Environmental Science, Institute of Life Science and Green Development, Hebei University, Baoding, 071002, China.
Qiu D; Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Key Laboratory of Analytical Science and Technology of Hebei Province, College of Chemistry and Environmental Science, Institute of Life Science and Green Development, Hebei University, Baoding, 071002, China.
Lv X; Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Key Laboratory of Analytical Science and Technology of Hebei Province, College of Chemistry and Environmental Science, Institute of Life Science and Green Development, Hebei University, Baoding, 071002, China.
Jiang Q; Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Key Laboratory of Analytical Science and Technology of Hebei Province, College of Chemistry and Environmental Science, Institute of Life Science and Green Development, Hebei University, Baoding, 071002, China.
Cheng Y; Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Key Laboratory of Analytical Science and Technology of Hebei Province, College of Chemistry and Environmental Science, Institute of Life Science and Green Development, Hebei University, Baoding, 071002, China. .
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Źródło:
Analytical and bioanalytical chemistry [Anal Bioanal Chem] 2021 Jan; Vol. 413 (1), pp. 255-261. Date of Electronic Publication: 2020 Oct 20.
Typ publikacji:
Journal Article
MeSH Terms:
Ligase Chain Reaction/*methods
Uracil-DNA Glycosidase/*analysis
HeLa Cells ; Hot Temperature ; Humans ; Limit of Detection ; Reproducibility of Results ; Uracil-DNA Glycosidase/antagonists & inhibitors
Czasopismo naukowe
Tytuł:
Simplified Paper Format for Detecting HIV Drug Resistance in Clinical Specimens by Oligonucleotide Ligation.
Autorzy:
Panpradist N; Department of Bioengineering, University of Washington, Seattle, Washington, United States of America.
Beck IA; Seattle Children's Research Institute, Seattle, Washington, United States of America.
Chung MH; Department of Internal Medicine, University of Washington, Seattle, Washington, United States of America.; Department of Global Health, University of Washington, Seattle, Washington, United States of America.
Kiarie JN; Departments of Obstetrics and Gynecology, University of Nairobi, Nairobi, Kenya.
Frenkel LM; Department of Global Health, University of Washington, Seattle, Washington, United States of America.; Departments of Pediatrics and Laboratory Medicine, University of Washington, Seattle, Washington, United States of America.
Lutz BR; Department of Bioengineering, University of Washington, Seattle, Washington, United States of America.
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Źródło:
PloS one [PLoS One] 2016 Jan 11; Vol. 11 (1), pp. e0145962. Date of Electronic Publication: 2016 Jan 11 (Print Publication: 2016).
Typ publikacji:
Journal Article; Observational Study; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
MeSH Terms:
Genotyping Techniques*
Molecular Diagnostic Techniques*
Point Mutation*
Drug Resistance, Viral/*genetics
HIV-1/*genetics
Ligase Chain Reaction/*methods
Anti-HIV Agents/pharmacology ; Dried Blood Spot Testing/economics ; Dried Blood Spot Testing/methods ; Drug Resistance, Viral/drug effects ; Genotype ; HIV Infections/diagnosis ; HIV Infections/drug therapy ; HIV Infections/virology ; Humans ; Kenya ; Ligase Chain Reaction/economics ; Oligonucleotide Probes/chemistry ; Paper ; Retrospective Studies ; Signal-To-Noise Ratio ; pol Gene Products, Human Immunodeficiency Virus/genetics
Czasopismo naukowe
Tytuł:
"Missing mutations" in MPS I: Identification of two novel copy number variations by an IDUA-specific in house MLPA assay.
Autorzy:
Jahic A; Institute of Clinical Chemistry and Laboratory Diagnostics, Jena University Hospital, Jena, Germany.; Institute of Laboratory Medicine, Clinical Chemistry and Pathobiochemistry, Charité - Universitätsmedizin Berlin, Berlin, Germany.
Günther S; Institute of Clinical Chemistry and Laboratory Diagnostics, Jena University Hospital, Jena, Germany.
Muschol N; Department of Pediatrics, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
Fossøy Stadheim B; Department of Clinical Genetics, Oslo University Hospital, Oslo, Norway.
Braaten Ø; Department of Clinical Genetics, Oslo University Hospital, Oslo, Norway.
Kjensli Hyldebrandt H; Department of Clinical Genetics, Oslo University Hospital, Oslo, Norway.
Kuiper GA; Pediatric Metabolic Diseases, Amsterdam UMC, University of Amsterdam, Academic Medical Center (AMC), Amsterdam, Netherlands.
Tylee K; Manchester Center for Genomic Medicine, St Mary's Hospital, Manchester, UK.
Wijburg FA; Pediatric Metabolic Diseases, Amsterdam UMC, University of Amsterdam, Academic Medical Center (AMC), Amsterdam, Netherlands.
Beetz C; Institute of Clinical Chemistry and Laboratory Diagnostics, Jena University Hospital, Jena, Germany.; Centogene AG, Rostock, Germany.
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Źródło:
Molecular genetics & genomic medicine [Mol Genet Genomic Med] 2019 Sep; Vol. 7 (9), pp. e00615. Date of Electronic Publication: 2019 Jul 18.
Typ publikacji:
Clinical Trial; Journal Article
MeSH Terms:
DNA Copy Number Variations*
Mutation*
Iduronidase/*genetics
Mucopolysaccharidosis I/*genetics
Female ; Humans ; Ligase Chain Reaction ; Male ; Mucopolysaccharidosis I/enzymology
Czasopismo naukowe
Tytuł:
Multiplexed, ligation-dependent probe amplification for rapid and inexpensive HLA-DQB1 allelotyping.
Autorzy:
Akers NK; Division of Environmental Health Sciences, School of Public Health, University of California, Berkeley, Berkeley, CA, USA. />Curry JD
Smith MT
Bracci PM
Skibola CF
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Źródło:
Tissue antigens [Tissue Antigens] 2011 Oct; Vol. 78 (4), pp. 275-80. Date of Electronic Publication: 2011 Jul 18.
Typ publikacji:
Journal Article; Research Support, N.I.H., Extramural
MeSH Terms:
Alleles*
HLA-DQ beta-Chains/*genetics
Histocompatibility Testing/*methods
Ligase Chain Reaction/*methods
Oligonucleotide Probes/*chemistry
Cell Line ; Female ; Heterozygote ; Histocompatibility Testing/economics ; Humans ; Ligase Chain Reaction/economics ; Male
Czasopismo naukowe
Tytuł:
Novel quantitative real-time LCR for the sensitive detection of SNP frequencies in pooled DNA: method development, evaluation and application.
Autorzy:
Psifidi A; Department of Animal Production, Faculty of Veterinary Medicine, Aristotle University of Thessaloniki, Thessaloniki, Greece.
Dovas C
Banos G
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Źródło:
PloS one [PLoS One] 2011 Jan 19; Vol. 6 (1), pp. e14560. Date of Electronic Publication: 2011 Jan 19.
Typ publikacji:
Evaluation Study; Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
Gene Frequency*
Polymorphism, Single Nucleotide*
DNA Mutational Analysis/*methods
Ligase Chain Reaction/*methods
Animals ; DNA/genetics ; DNA Mutational Analysis/standards ; Fluorescent Dyes ; Ligase Chain Reaction/standards ; Limit of Detection ; Methods ; Reproducibility of Results ; Sensitivity and Specificity ; Sheep
Czasopismo naukowe
Tytuł:
HER-2/neu amplification testing in breast cancer by multiplex ligation-dependent probe amplification in comparison with immunohistochemistry and in situ hybridization.
Autorzy:
Moelans CB; Department of Pathology, University Medical Centre Utrecht, Heidelberglaan 100, Utrecht, The Netherlands.
de Weger RA
van Blokland MT
Ezendam C
Elshof S
Tilanus MG
van Diest PJ
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Źródło:
Cellular oncology : the official journal of the International Society for Cellular Oncology [Cell Oncol] 2009; Vol. 31 (1), pp. 1-10.
Typ publikacji:
Comparative Study; Journal Article
MeSH Terms:
Gene Amplification*
Breast Neoplasms/*genetics
Immunohistochemistry/*methods
In Situ Hybridization, Fluorescence/*methods
Ligase Chain Reaction/*methods
Receptor, ErbB-2/*genetics
Breast Neoplasms/metabolism ; Female ; Humans ; Ligase Chain Reaction/economics ; Molecular Probes/genetics ; Receptor, ErbB-2/metabolism
Czasopismo naukowe
Tytuł:
Search for copy number alterations in the MEFV gene using multiplex ligation probe amplification, experience from three diagnostic centres.
Autorzy:
van Gijn ME; DBG Department of Medical Genetics, UMC Utrecht, The Netherlands. />Soler S
de la Chapelle C
Mulder M
Ritorre C
Kriek M
Philibert L
van der Wielen M
Frenkel J
Grandemange S
Bakker E
Ploos van Amstel JK
Touitou I
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Źródło:
European journal of human genetics : EJHG [Eur J Hum Genet] 2008 Nov; Vol. 16 (11), pp. 1404-6. Date of Electronic Publication: 2008 Jul 23.
Typ publikacji:
Journal Article; Multicenter Study; Research Support, Non-U.S. Gov't
MeSH Terms:
Ligase Chain Reaction*
Point Mutation*
Cytoskeletal Proteins/*genetics
Familial Mediterranean Fever/*genetics
Cohort Studies ; Female ; Humans ; Male ; Pyrin
Czasopismo naukowe
Tytuł:
Computer-assisted prenatal aneuploidy screening for chromosome 13, 18, 21, X and Y based on multiplex ligation-dependent probe amplification (MLPA).
Autorzy:
Gerdes T; Department of Clinical Genetics, Rigshospitalet, DK-2100 Copenhagen Ø, Denmark.
Kirchhoff M
Lind AM
Larsen GV
Schwartz M
Lundsteen C
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Źródło:
European journal of human genetics : EJHG [Eur J Hum Genet] 2005 Feb; Vol. 13 (2), pp. 171-5.
Typ publikacji:
Journal Article
MeSH Terms:
Aneuploidy*
Genetic Testing*/methods
Ligase Chain Reaction*/methods
Prenatal Diagnosis*/methods
Chromosomes, Human/*genetics
False Negative Reactions ; False Positive Reactions ; Female ; Humans ; Predictive Value of Tests ; Pregnancy
Czasopismo naukowe
Tytuł:
Use of ligase chain reaction for the rapid diagnosis of lymph node tuberculosis.
Autorzy:
León Muiños E; Department of Microbiology, Clinical University Hospital, Santiago de Compostela, A Coruña, Spain.
Pérez Del Molino ML
Lado Lado FL
Pazo Nuñez M
Pardo F
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Źródło:
Scandinavian journal of infectious diseases [Scand J Infect Dis] 2004; Vol. 36 (10), pp. 724-6.
Typ publikacji:
Comparative Study; Journal Article
MeSH Terms:
Ligase Chain Reaction*
Mycobacterium tuberculosis/*isolation & purification
Tuberculosis, Lymph Node/*diagnosis
Adolescent ; Adult ; Aged ; Aged, 80 and over ; Bacteriological Techniques ; Child ; Child, Preschool ; Cohort Studies ; DNA Ligases/analysis ; DNA, Bacterial ; Female ; Humans ; Male ; Middle Aged ; Sensitivity and Specificity ; Spain ; Sputum/microbiology ; Tissue Culture Techniques
Czasopismo naukowe
Tytuł:
Rapid diagnosis of tuberculous meningitis by ligase chain reaction amplification.
Autorzy:
Rajo MC; Servicio de Microbiologia, Hospital Clinico Universitario de Santiago de Compostela, Spain. />Pérez Del Molina ML
Lado Lado FL
López MJ
Prieto E
Pardo F
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Źródło:
Scandinavian journal of infectious diseases [Scand J Infect Dis] 2002; Vol. 34 (1), pp. 14-6.
Typ publikacji:
Evaluation Study; Journal Article
MeSH Terms:
Ligase Chain Reaction*
Mycobacterium tuberculosis/*isolation & purification
Tuberculosis, Meningeal/*diagnosis
Cerebrospinal Fluid/microbiology ; Humans ; Mycobacterium tuberculosis/genetics ; Nucleic Acid Amplification Techniques/methods ; Predictive Value of Tests ; Sensitivity and Specificity ; Tuberculosis, Meningeal/microbiology
Czasopismo naukowe
Tytuł:
Cervical, urine and vaginal specimens for detection of Chlamydia trachomatis by ligase chain reaction in women: a comparison.
Autorzy:
Hjelm E; Department of Medical Sciences, University Hospital, Uppsala, Sweden. />Hallén A
Domeika M
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Źródło:
Acta dermato-venereologica [Acta Derm Venereol] 2001 Aug-Sep; Vol. 81 (4), pp. 285-8.
Typ publikacji:
Comparative Study; Journal Article
MeSH Terms:
Ligase Chain Reaction*
Cervix Uteri/*microbiology
Chlamydia Infections/*microbiology
Chlamydia Infections/*urine
Chlamydia trachomatis/*isolation & purification
Vagina/*microbiology
Adolescent ; Adult ; Female ; Humans ; Middle Aged ; Predictive Value of Tests ; Sensitivity and Specificity ; Urine/microbiology
Czasopismo naukowe
Czasopismo naukowe
Tytuł:
Alternative nucleic amplification strategies, part 2: LCR and HDA.
Autorzy:
Brunstein J
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Źródło:
MLO: medical laboratory observer [MLO Med Lab Obs] 2014 Feb; Vol. 46 (2), pp. 22, 24-5.
Typ publikacji:
Journal Article
MeSH Terms:
DNA Helicases/*metabolism
Ligase Chain Reaction/*methods
DNA Primers/metabolism ; DNA Replication/physiology ; DNA-Binding Proteins/metabolism ; Molecular Diagnostic Techniques ; Nucleic Acid Amplification Techniques/methods ; Polymorphism, Single Nucleotide ; Temperature ; Transcription, Genetic ; United States
Czasopismo naukowe
Tytuł:
Multiplex ligation-dependent probe amplification to detect HER2 amplification in breast cancer: new insights in optimal cut-off value.
Autorzy:
Moelans CB
de Weger RA
van Diest PJ
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Źródło:
Cellular oncology : the official journal of the International Society for Cellular Oncology [Cell Oncol] 2010 Jan 01; Vol. 32 (4), pp. 311-2.
Typ publikacji:
Comment; Letter
MeSH Terms:
Early Detection of Cancer*
Reference Standards*
Breast Neoplasms/*diagnosis
Ligase Chain Reaction/*methods
Receptor, ErbB-2/*genetics
Breast Neoplasms/genetics ; Female ; Gene Amplification/genetics ; Humans ; Oligonucleotide Probes ; Reproducibility of Results
Opinia redakcyjna
Tytuł:
MALS: an efficient strategy for multiple site-directed mutagenesis employing a combination of DNA amplification, ligation and suppression PCR.
Autorzy:
Fushan AA; National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Bethesda, MD 20892, USA. />Drayna DT
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Źródło:
BMC biotechnology [BMC Biotechnol] 2009 Sep 24; Vol. 9, pp. 83. Date of Electronic Publication: 2009 Sep 24.
Typ publikacji:
Journal Article; Research Support, N.I.H., Extramural
MeSH Terms:
Ligase Chain Reaction/*methods
Mutagenesis, Site-Directed/*methods
Polymerase Chain Reaction/*methods
Mutation
Czasopismo naukowe
Tytuł:
High sensitivity detection of Plasmodium species reveals positive correlations between infections of different species, shifts in age distribution and reduced local variation in Papua New Guinea.
Autorzy:
Mueller I; Papua New Guinea Institute of Medical Research, Goroka, Papua New Guinea. />Widmer S
Michel D
Maraga S
McNamara DT
Kiniboro B
Sie A
Smith TA
Zimmerman PA
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Źródło:
Malaria journal [Malar J] 2009 Mar 11; Vol. 8, pp. 41. Date of Electronic Publication: 2009 Mar 11.
Typ publikacji:
Journal Article; Research Support, N.I.H., Extramural
MeSH Terms:
DNA, Protozoan/*genetics
Malaria/*diagnosis
Malaria/*parasitology
Plasmodium/*classification
Plasmodium/*growth & development
Adolescent ; Age Distribution ; Animals ; Child ; Child, Preschool ; Cross-Sectional Studies ; Female ; Humans ; Ligase Chain Reaction ; Malaria/epidemiology ; Male ; Microspheres ; Molecular Sequence Data ; Papua New Guinea/epidemiology ; Plasmodium/genetics ; Polymerase Chain Reaction/methods ; Population Surveillance/methods ; Prevalence ; Sensitivity and Specificity ; Young Adult
Czasopismo naukowe
Tytuł:
Unambiguous molecular detections with multiple genetic approach for the complicated chromosome 22q11 deletion syndrome.
Autorzy:
Yang C; Division of Genetics, Department of Pediatrics, Taipei Medical University Hospital, Taipei 11031, Taiwan. />Huang CH
Cheong ML
Hung KL
Lin LH
Yu YS
Chien CC
Huang HC
Chen CW
Huang CJ
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Źródło:
BMC medical genetics [BMC Med Genet] 2009 Feb 25; Vol. 10, pp. 16. Date of Electronic Publication: 2009 Feb 25.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
Chromosome Deletion*
Chromosomes, Human, Pair 22*
Abnormalities, Multiple/genetics ; Child ; Child, Preschool ; DNA Primers ; Female ; Genetic Markers ; Genome, Human ; Heart Defects, Congenital/genetics ; Humans ; Infant ; Infant, Newborn ; Ligase Chain Reaction ; Male ; Polymerase Chain Reaction ; Syndrome ; Taiwan
Czasopismo naukowe
Tytuł:
High-resolution array copy number analyses for detection of deletion, gain, amplification and copy-neutral LOH in primary neuroblastoma tumors: four cases of homozygous deletions of the CDKN2A gene.
Autorzy:
Carén H; Department of Clinical Genetics, Institute of Biomedicine, Göteborg University, Sahlgrenska University Hospital, SE-41345 Göteborg, Sweden. />Erichsen J
Olsson L
Enerbäck C
Sjöberg RM
Abrahamsson J
Kogner P
Martinsson T
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Źródło:
BMC genomics [BMC Genomics] 2008 Jul 29; Vol. 9, pp. 353. Date of Electronic Publication: 2008 Jul 29.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
Chromosome Deletion*
Computational Biology*
Gene Dosage*
Loss of Heterozygosity*
Cyclin-Dependent Kinase Inhibitor p16/*genetics
Neuroblastoma/*genetics
Cell Line, Tumor ; Child ; Chromosome Mapping ; Chromosomes, Human, Pair 9/genetics ; Gene Amplification ; Gene Deletion ; Gene Rearrangement ; Homozygote ; Humans ; Ligase Chain Reaction ; N-Myc Proto-Oncogene Protein ; Nuclear Proteins/genetics ; Oligonucleotide Array Sequence Analysis ; Oncogene Proteins/genetics ; Polymorphism, Single Nucleotide
Czasopismo naukowe

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