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    Tytuł:
    Correlative three-dimensional super-resolution and block-face electron microscopy of whole vitreously frozen cells.
    Autorzy:
    Hoffman DP; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA.
    Shtengel G; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA.
    Xu CS; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA.
    Campbell KR; Department of Developmental Neurobiology, St. Jude Children's Research Hospital, Memphis, TN 38105, USA.
    Freeman M; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA.
    Wang L; Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA.; Program in Cellular and Molecular Medicine, Boston Children's Hospital, Boston, MA 02115, USA.; Department of Pediatrics, Harvard Medical School, Boston, MA 02115, USA.
    Milkie DE; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA.
    Pasolli HA; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA.
    Iyer N; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA.
    Bogovic JA; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA.
    Stabley DR; Neuroimaging Laboratory, St. Jude Children's Research Hospital, Memphis, TN 38105, USA.
    Shirinifard A; Bioimage Analysis Core, St. Jude Children's Research Hospital, Memphis, TN 38105, USA.
    Pang S; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA.
    Peale D; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA.
    Schaefer K; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA.
    Pomp W; Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA.; Program in Cellular and Molecular Medicine, Boston Children's Hospital, Boston, MA 02115, USA.; Department of Pediatrics, Harvard Medical School, Boston, MA 02115, USA.
    Chang CL; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA.
    Lippincott-Schwartz J; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA.
    Kirchhausen T; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA.; Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA.; Program in Cellular and Molecular Medicine, Boston Children's Hospital, Boston, MA 02115, USA.; Department of Pediatrics, Harvard Medical School, Boston, MA 02115, USA.
    Solecki DJ; Department of Developmental Neurobiology, St. Jude Children's Research Hospital, Memphis, TN 38105, USA.
    Betzig E; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA. .; Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA.; Department of Physics, University of California, Berkeley, CA 94720, USA.; Howard Hughes Medical Institute, Berkeley, CA 94720, USA.; Helen Wills Neuroscience Institute, Berkeley, CA 94720, USA.; Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
    Hess HF; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA. .
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    Źródło:
    Science (New York, N.Y.) [Science] 2020 Jan 17; Vol. 367 (6475).
    Typ publikacji:
    Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
    MeSH Terms:
    Cells/*ultrastructure
    Cryoelectron Microscopy/*methods
    Imaging, Three-Dimensional/*methods
    Microscopy, Fluorescence/*methods
    Animals ; COS Cells ; Cell Adhesion ; Cell Line, Tumor ; Chlorocebus aethiops ; Freezing ; HeLa Cells ; Humans ; Mice
    Czasopismo naukowe
    Pełny tekst  Link otwiera się w nowym oknie Pełny tekstSzczegóły
    Tytuł:
    Photoactivatable mCherry for high-resolution two-color fluorescence microscopy.
    Autorzy:
    Subach FV; Department of Anatomy and Structural Biology, and Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, 1300 Morris Park Ave., Bronx, New York 10461, USA.
    Patterson GH
    Manley S
    Gillette JM
    Lippincott-Schwartz J
    Verkhusha VV
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    Źródło:
    Nature methods [Nat Methods] 2009 Feb; Vol. 6 (2), pp. 153-9. Date of Electronic Publication: 2009 Jan 25.
    Typ publikacji:
    Journal Article; Research Support, N.I.H., Extramural
    MeSH Terms:
    Fluorescent Dyes/*chemistry
    Luminescent Proteins/*chemistry
    Microscopy, Fluorescence/*methods
    Luminescent Proteins/genetics ; Microscopy, Confocal/methods ; Mutagenesis, Site-Directed ; Photochemical Processes ; Red Fluorescent Protein
    Czasopismo naukowe
    Pełny tekst  Link otwiera się w nowym oknieSzczegóły
    Tytuł:
    Putting super-resolution fluorescence microscopy to work.
    Autorzy:
    Lippincott-Schwartz J; Cell Biology and Metabolism Program, National Institute of Child Health and Human Development, National Institutes of Health, 18 Library Drive, Bethesda, MD 20892, USA. />Manley S
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    Źródło:
    Nature methods [Nat Methods] 2009 Jan; Vol. 6 (1), pp. 21-3.
    Typ publikacji:
    Journal Article
    MeSH Terms:
    Microscopy, Fluorescence/*instrumentation
    Microscopy, Fluorescence/*methods
    Animals ; Biology ; Computer Graphics ; Computer-Aided Design
    Czasopismo naukowe
    Pełny tekst  Link otwiera się w nowym oknieSzczegóły
    Tytuł:
    High-density mapping of single-molecule trajectories with photoactivated localization microscopy.
    Autorzy:
    Manley S; National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA.
    Gillette JM
    Patterson GH
    Shroff H
    Hess HF
    Betzig E
    Lippincott-Schwartz J
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    Źródło:
    Nature methods [Nat Methods] 2008 Feb; Vol. 5 (2), pp. 155-7. Date of Electronic Publication: 2008 Jan 13.
    Typ publikacji:
    Journal Article; Research Support, N.I.H., Intramural; Research Support, Non-U.S. Gov't
    MeSH Terms:
    Image Enhancement/*methods
    Membrane Proteins/*metabolism
    Membrane Proteins/*ultrastructure
    Microscopy, Fluorescence/*methods
    Animals ; COS Cells ; Chlorocebus aethiops
    Czasopismo naukowe
    Pełny tekst  Link otwiera się w nowym oknieSzczegóły
    Tytuł:
    Development and use of fluorescent protein markers in living cells.
    Autorzy:
    Lippincott-Schwartz J; Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA. />Patterson GH
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    Źródło:
    Science (New York, N.Y.) [Science] 2003 Apr 04; Vol. 300 (5616), pp. 87-91.
    Typ publikacji:
    Journal Article; Review
    MeSH Terms:
    Cell Physiological Phenomena*
    Luminescent Proteins*/chemistry
    Luminescent Proteins*/genetics
    Luminescent Proteins*/metabolism
    Diagnostic Imaging/*methods
    Microscopy/*methods
    Microscopy, Fluorescence/*methods
    Proteins/*metabolism
    Fluorescence ; Fluorescence Recovery After Photobleaching/methods ; Fluorometry/methods ; Green Fluorescent Proteins ; Kinetics ; Light ; Mutagenesis ; Protein Engineering ; Recombinant Fusion Proteins ; Spectrometry, Fluorescence
    Czasopismo naukowe
    Pełny tekst  Link otwiera się w nowym oknie Pełny tekstSzczegóły
      Wyświetlanie 1-5 z 5

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