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Tytuł :
Detecting SARS-CoV-2 3CLpro expression and activity using a polyclonal antiserum and a luciferase-based biosensor.
Autorzy :
O'Brien A; Department of Microbiology and Immunology, Loyola University Chicago, Stritch School of Medicine, Maywood, IL, USA.
Chen DY; Department of Biochemistry, Boston University School of Medicine, Boston, MA, USA; National Emerging Infectious Diseases Laboratories, Boston University, MA, USA.
Hackbart M; Department of Microbiology and Immunology, Loyola University Chicago, Stritch School of Medicine, Maywood, IL, USA.
Close BJ; National Emerging Infectious Diseases Laboratories, Boston University, MA, USA; Department of Microbiology, Boston University School of Medicine, Boston, MA, USA.
O'Brien TE; Department of Mathematics and Statistics, Loyola University Chicago, Chicago, IL, USA.
Saeed M; Department of Biochemistry, Boston University School of Medicine, Boston, MA, USA; National Emerging Infectious Diseases Laboratories, Boston University, MA, USA. Electronic address: .
Baker SC; Department of Microbiology and Immunology, Loyola University Chicago, Stritch School of Medicine, Maywood, IL, USA. Electronic address: .
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Źródło :
Virology [Virology] 2021 Apr; Vol. 556, pp. 73-78. Date of Electronic Publication: 2021 Jan 26.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
MeSH Terms :
Biosensing Techniques/*methods
Coronavirus 3C Proteases/*metabolism
Immune Sera/*immunology
Luciferases/*metabolism
SARS-CoV-2/*metabolism
Animals ; Antiviral Agents/pharmacology ; Coronavirus 3C Proteases/antagonists & inhibitors ; Coronavirus 3C Proteases/genetics ; Coronavirus 3C Proteases/immunology ; Cross Reactions ; Luciferases/genetics ; Protease Inhibitors/pharmacology ; Rabbits ; Recombinant Fusion Proteins/genetics ; Recombinant Fusion Proteins/metabolism ; SARS Virus/immunology ; SARS Virus/metabolism ; SARS-CoV-2/drug effects ; SARS-CoV-2/genetics ; SARS-CoV-2/immunology ; Viral Nonstructural Proteins/genetics ; Viral Nonstructural Proteins/immunology ; Viral Nonstructural Proteins/metabolism ; Virus Replication/drug effects
Czasopismo naukowe
Tytuł :
Unravelling the mechanism of pH-regulation in dinoflagellate luciferase.
Autorzy :
Kamerlin N; Science for Life Laboratory, Department of Cell and Molecular Biology, Uppsala University, SE-751 05 Uppsala, Sweden. Electronic address: .
Delcey MG; Department of Chemistry - Ångström Laboratory, Uppsala University, SE-751 02 Uppsala, Sweden.
Lindh R; Department of Chemistry - BMC, Uppsala University, P.O. Box 576, SE-751 23 Uppsala, Sweden.
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Źródło :
International journal of biological macromolecules [Int J Biol Macromol] 2020 Dec 01; Vol. 164, pp. 2671-2680. Date of Electronic Publication: 2020 Aug 19.
Typ publikacji :
Journal Article
MeSH Terms :
Dinoflagellida/*enzymology
Luciferases/*chemistry
Luciferases/*metabolism
Dinoflagellida/chemistry ; Dinoflagellida/genetics ; Hydrogen-Ion Concentration ; Luciferases/genetics ; Models, Molecular ; Molecular Dynamics Simulation ; Mutation ; Protein Conformation ; Protein Domains ; Protozoan Proteins/chemistry ; Protozoan Proteins/metabolism
Czasopismo naukowe
Tytuł :
Modeling Brain Metastasis Via Tail-Vein Injection of Inflammatory Breast Cancer Cells.
Autorzy :
Hu X; Department of Breast Medical Oncology, The University of Texas MD Anderson Cancer Center; Morgan Welch Inflammatory Breast Cancer Clinic and Research Program, The University of Texas MD Anderson Cancer Center.
Villodre ES; Department of Breast Medical Oncology, The University of Texas MD Anderson Cancer Center; Morgan Welch Inflammatory Breast Cancer Clinic and Research Program, The University of Texas MD Anderson Cancer Center.
Woodward WA; Morgan Welch Inflammatory Breast Cancer Clinic and Research Program, The University of Texas MD Anderson Cancer Center; Department of Radiation Oncology, The University of Texas MD Anderson Cancer Center.
Debeb BG; Department of Breast Medical Oncology, The University of Texas MD Anderson Cancer Center; Morgan Welch Inflammatory Breast Cancer Clinic and Research Program, The University of Texas MD Anderson Cancer Center; .
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Źródło :
Journal of visualized experiments : JoVE [J Vis Exp] 2021 Feb 04 (168). Date of Electronic Publication: 2021 Feb 04.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Video-Audio Media
MeSH Terms :
Brain Neoplasms/*secondary
Cell Tracking/*methods
Inflammatory Breast Neoplasms/*pathology
Injections, Intravenous/*methods
Luciferases, Firefly/*metabolism
Animals ; Female ; Humans ; Luciferases, Firefly/genetics ; Mice ; Tail ; Tumor Cells, Cultured ; Xenograft Model Antitumor Assays
Czasopismo naukowe
Tytuł :
Development of a Cell-Based Luciferase Complementation Assay for Identification of SARS-CoV-2 3CL Inhibitors.
Autorzy :
Rawson JMO; Viral Recombination Section, HIV Dynamics and Replication Program, National Cancer Institute, Frederick, MD 21702, USA.
Duchon A; Viral Recombination Section, HIV Dynamics and Replication Program, National Cancer Institute, Frederick, MD 21702, USA.
Nikolaitchik OA; Viral Recombination Section, HIV Dynamics and Replication Program, National Cancer Institute, Frederick, MD 21702, USA.
Pathak VK; Viral Mutation Section, HIV Dynamics and Replication Program, National Cancer Institute, Frederick, MD 21702, USA.
Hu WS; Viral Recombination Section, HIV Dynamics and Replication Program, National Cancer Institute, Frederick, MD 21702, USA.
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Źródło :
Viruses [Viruses] 2021 Jan 24; Vol. 13 (2). Date of Electronic Publication: 2021 Jan 24.
Typ publikacji :
Journal Article; Research Support, N.I.H., Intramural
MeSH Terms :
Antiviral Agents/*metabolism
Coronavirus 3C Proteases/*antagonists & inhibitors
Luciferases/*metabolism
Protease Inhibitors/*metabolism
SARS-CoV-2/*enzymology
Antiviral Agents/pharmacology ; Cell Survival/drug effects ; Coronavirus 3C Proteases/genetics ; Coronavirus 3C Proteases/metabolism ; Drug Evaluation, Preclinical ; HEK293 Cells ; Humans ; Lentivirus/genetics ; Luciferases/genetics ; Protease Inhibitors/pharmacology
Czasopismo naukowe
Tytuł :
Development of a nano-luciferase based assay to measure the binding of SARS-CoV-2 spike receptor binding domain to ACE-2.
Autorzy :
Lima MA; Molecular & Structural Bioscience, School of Life Sciences, Keele University Staffordshire, ST5 5BG, United Kingdom.
Skidmore M; Molecular & Structural Bioscience, School of Life Sciences, Keele University Staffordshire, ST5 5BG, United Kingdom.
Khanim F; School of Biomedical Sciences, Institute of Clinical Sciences Medical School University of Birmingham, Birmingham, B15 2TT, United Kingdom.
Richardson A; School of Pharmacy and Bioengineering, Keele University Staffordshire, ST5 5BG, United Kingdom. Electronic address: .
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Źródło :
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2021 Jan 01; Vol. 534, pp. 485-490. Date of Electronic Publication: 2020 Nov 17.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Angiotensin-Converting Enzyme 2/*metabolism
Luciferases/*metabolism
Receptors, Virus/*metabolism
Spike Glycoprotein, Coronavirus/*metabolism
Angiotensin-Converting Enzyme 2/chemistry ; Angiotensin-Converting Enzyme 2/genetics ; Antiviral Agents/metabolism ; Antiviral Agents/therapeutic use ; Binding Sites/genetics ; COVID-19/drug therapy ; COVID-19/metabolism ; COVID-19/virology ; Humans ; Luciferases/genetics ; Nanotechnology/methods ; Protein Binding ; Protein Domains ; Receptors, Virus/genetics ; SARS-CoV-2/drug effects ; SARS-CoV-2/metabolism ; SARS-CoV-2/physiology ; Spike Glycoprotein, Coronavirus/genetics
Czasopismo naukowe
Tytuł :
Construction of CRISPR Plasmids and Detection of Knockout Efficiency in Mammalian Cells through a Dual Luciferase Reporter System.
Autorzy :
Li H; Qingdao Agricultural University, Qingdao, China.
Qin H; Qingdao Agricultural University, Qingdao, China.
Zhang N; Qingdao Agricultural University, Qingdao, China.
Zhao J; Qingdao Agricultural University, Qingdao, China.
Xin J; Qingdao Agricultural University, Qingdao, China.
Perez-Campo FM; University of Cantabria, Santander y Torrelavega, Spain.
Liu H; Qingdao Agricultural University, Qingdao, China; .
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Źródło :
Journal of visualized experiments : JoVE [J Vis Exp] 2020 Dec 05 (166). Date of Electronic Publication: 2020 Dec 05.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't; Video-Audio Media
MeSH Terms :
Gene Knockout Techniques*
Genes, Reporter*
CRISPR-Cas Systems/*genetics
Luciferases/*metabolism
Mammals/*metabolism
Plasmids/*genetics
Animals ; Base Sequence ; Cell Line ; DNA/metabolism ; DNA Repair ; Genetic Vectors/metabolism ; Luciferases/genetics ; Oligonucleotides/metabolism ; RNA, Guide/genetics ; Reproducibility of Results ; Sheep ; Transformation, Genetic
Czasopismo naukowe
Tytuł :
Luminescence of Cypridina Luciferin in the Presence of Human Plasma Alpha 1-Acid Glycoprotein.
Autorzy :
Kanie S; Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Hokkaido Center, Sapporo 062-8517, Japan.
Komatsu M; Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Hokkaido Center, Sapporo 062-8517, Japan.
Mitani Y; Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Hokkaido Center, Sapporo 062-8517, Japan.
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Źródło :
International journal of molecular sciences [Int J Mol Sci] 2020 Oct 12; Vol. 21 (20). Date of Electronic Publication: 2020 Oct 12.
Typ publikacji :
Journal Article
MeSH Terms :
Luminescence*
Luminescent Measurements*/methods
Luciferases/*metabolism
Orosomucoid/*metabolism
Humans ; Hydrogen-Ion Concentration ; Luciferases/chemistry ; Luciferases/genetics ; Molecular Structure ; Temperature
Czasopismo naukowe
Tytuł :
Super-rapid quantitation of the production of HIV-1 harboring a luminescent peptide tag.
Autorzy :
Ozono S; Department of Pathology, National Institute of Infectious Diseases, Tokyo, Japan; Faculty of Life and Environmental Sciences, University of Yamanashi, Yamanashi, Japan.
Zhang Y; Department of Pathology, National Institute of Infectious Diseases, Tokyo, Japan.
Tobiume M; Department of Pathology, National Institute of Infectious Diseases, Tokyo, Japan.
Kishigami S; Faculty of Life and Environmental Sciences, University of Yamanashi, Yamanashi, Japan.
Tokunaga K; Department of Pathology, National Institute of Infectious Diseases, Tokyo, Japan. Electronic address: .
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Źródło :
The Journal of biological chemistry [J Biol Chem] 2020 Sep 11; Vol. 295 (37), pp. 13023-13030. Date of Electronic Publication: 2020 Jul 20.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Genetic Vectors*/genetics
Genetic Vectors*/metabolism
HIV-1*/genetics
HIV-1*/metabolism
Luciferases*/genetics
Luciferases*/metabolism
Peptides*/genetics
Peptides*/metabolism
HeLa Cells ; Humans
Czasopismo naukowe
Tytuł :
Inhibition of firefly luciferase activity by a HIF prolyl hydroxylase inhibitor.
Autorzy :
Günter J; Institute of Physiology, University of Zurich, Zurich, & National Centre of Competence in Research 'Kidney.CH', Zurich, Switzerland.
Wenger RH; Institute of Physiology, University of Zurich, Zurich, & National Centre of Competence in Research 'Kidney.CH', Zurich, Switzerland.
Scholz CC; Institute of Physiology, University of Zurich, Zurich, & National Centre of Competence in Research 'Kidney.CH', Zurich, Switzerland. Electronic address: .
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Źródło :
Journal of photochemistry and photobiology. B, Biology [J Photochem Photobiol B] 2020 Sep; Vol. 210, pp. 111980. Date of Electronic Publication: 2020 Jul 27.
Typ publikacji :
Journal Article
MeSH Terms :
Luciferases, Firefly/*metabolism
Prolyl-Hydroxylase Inhibitors/*chemistry
Animals ; Benzothiazoles/metabolism ; Binding, Competitive ; Fireflies/enzymology ; Glycine/analogs & derivatives ; Glycine/chemistry ; Glycine/metabolism ; Isoquinolines/chemistry ; Isoquinolines/metabolism ; Kinetics ; Luciferases, Firefly/antagonists & inhibitors ; Luciferases, Firefly/genetics ; Prolyl-Hydroxylase Inhibitors/metabolism ; Recombinant Proteins/biosynthesis ; Recombinant Proteins/isolation & purification ; Renilla/enzymology
Czasopismo naukowe
Tytuł :
pH-Dependent absorption spectrum of oxyluciferin analogues in the active site of firefly luciferase.
Autorzy :
de Almeida Barbosa NM; Aix-Marseille Univ, CNRS, ICR, Marseille, France. .
Gosset P; Université de Strasbourg, UMR 7021 CNRS, Laboratoire de Bioimagerie et Pathologies, Strasbourg, France.
Réal E; Université de Strasbourg, UMR 7021 CNRS, Laboratoire de Bioimagerie et Pathologies, Strasbourg, France.
Ledentu V; Aix-Marseille Univ, CNRS, ICR, Marseille, France. .
Didier P; Université de Strasbourg, UMR 7021 CNRS, Laboratoire de Bioimagerie et Pathologies, Strasbourg, France.
Ferré N; Aix-Marseille Univ, CNRS, ICR, Marseille, France. .
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Źródło :
Physical chemistry chemical physics : PCCP [Phys Chem Chem Phys] 2020 Oct 07; Vol. 22 (38), pp. 21731-21740.
Typ publikacji :
Journal Article
MeSH Terms :
Indoles/*chemistry
Luciferases, Firefly/*chemistry
Pyrazines/*chemistry
Animals ; Catalytic Domain ; Hydrogen-Ion Concentration ; Luciferases, Firefly/metabolism ; Spectrometry, Fluorescence
Czasopismo naukowe
Tytuł :
A multiplexed bioluminescent reporter for sensitive and non-invasive tracking of DNA double strand break repair dynamics in vitro and in vivo.
Autorzy :
Chien JC; Institute of Atomic and Molecular Sciences, Academia Sinica, Taipei 10617, Taiwan.
Tabet E; Department of Neurology, Massachusetts General Hospital, Boston, MA 02129, USA.; Department of Biomedical Engineering, University of South Dakota, 4800 N. Career Ave, Suite 221, Sioux Falls, Vermillion, SD 57069, USA.
Pinkham K; Department of Neurology, Massachusetts General Hospital, Boston, MA 02129, USA.
da Hora CC; Department of Neurology, Massachusetts General Hospital, Boston, MA 02129, USA.; Neuroscience Program, Harvard Medical School, Boston, MA 02115, USA.
Chang JC; Institute of Biochemical Sciences, National Taiwan University, Taipei 10617, Taiwan.; Institute of Biological Chemistry, Academia Sinica, Taipei 115, Taiwan.
Lin S; Institute of Biochemical Sciences, National Taiwan University, Taipei 10617, Taiwan.; Institute of Biological Chemistry, Academia Sinica, Taipei 115, Taiwan.
Badr CE; Department of Neurology, Massachusetts General Hospital, Boston, MA 02129, USA.; Neuroscience Program, Harvard Medical School, Boston, MA 02115, USA.
Lai CP; Institute of Atomic and Molecular Sciences, Academia Sinica, Taipei 10617, Taiwan.; Chemical Biology and Molecular Biophysics Program, Taiwan International Graduate Program, Academia Sinica, Taipei 115, Taiwan.; Genome and Systems Biology Degree Program, National Taiwan University and Academia Sinica, Taipei 115, Taiwan.
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Źródło :
Nucleic acids research [Nucleic Acids Res] 2020 Sep 25; Vol. 48 (17), pp. e100.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
MeSH Terms :
Genes, Reporter*
Recombinational DNA Repair*
Luciferases/*genetics
Animals ; CRISPR-Cas Systems ; Cell Line, Tumor ; Copepoda/enzymology ; DNA End-Joining Repair ; Female ; HEK293 Cells ; High-Throughput Nucleotide Sequencing/methods ; Humans ; Luciferases/metabolism ; Mice ; Mice, Nude ; Multiplex Polymerase Chain Reaction/methods ; Optical Imaging/methods ; Rad51 Recombinase/genetics ; Rad51 Recombinase/metabolism ; Sequence Analysis, DNA/methods
Czasopismo naukowe
Tytuł :
Mechanically Assisted Bioluminescence with Natural Luciferase.
Autorzy :
Schramm S; New York University Abu Dhabi, POB 129188, Abu Dhabi, United Arab Emirates.
Al-Handawi MB; New York University Abu Dhabi, POB 129188, Abu Dhabi, United Arab Emirates.
Karothu DP; New York University Abu Dhabi, POB 129188, Abu Dhabi, United Arab Emirates.
Kurlevskaya A; New York University Abu Dhabi, POB 129188, Abu Dhabi, United Arab Emirates.
Commins P; New York University Abu Dhabi, POB 129188, Abu Dhabi, United Arab Emirates.
Mitani Y; Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2-17-2-1 Tsukisamu-higashi, Toyohira-ku, Sapporo, 062-8517, Japan.
Wu C; Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-8-31 Midorigaoka, Ikeda, 563-8577, Japan.
Ohmiya Y; DAILAB, Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-8-31 Midorigaoka, Ikeda, 563-8577, Japan.
Naumov P; New York University Abu Dhabi, POB 129188, Abu Dhabi, United Arab Emirates.; Radcliffe Institute for Advanced Study, Harvard University, 10 Garden St, Cambridge, MA, 02138, USA.
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Źródło :
Angewandte Chemie (International ed. in English) [Angew Chem Int Ed Engl] 2020 Sep 14; Vol. 59 (38), pp. 16485-16489. Date of Electronic Publication: 2020 Jul 21.
Typ publikacji :
Journal Article
MeSH Terms :
Luminescent Measurements*
Luciferases/*metabolism
Animals ; Crustacea ; Firefly Luciferin/chemistry ; Firefly Luciferin/metabolism ; Luciferases/chemistry ; Molecular Structure
Czasopismo naukowe
Tytuł :
A Split Luciferase Complementation Assay for the Quantification of β-Arrestin2 Recruitment to Dopamine D 2 -Like Receptors.
Autorzy :
Forster L; Institute of Pharmacy, University of Regensburg, D-93053 Regensburg, Germany.
Grätz L; Institute of Pharmacy, University of Regensburg, D-93053 Regensburg, Germany.
Mönnich D; Institute of Pharmacy, University of Regensburg, D-93053 Regensburg, Germany.
Bernhardt G; Institute of Pharmacy, University of Regensburg, D-93053 Regensburg, Germany.
Pockes S; Institute of Pharmacy, University of Regensburg, D-93053 Regensburg, Germany.
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Źródło :
International journal of molecular sciences [Int J Mol Sci] 2020 Aug 24; Vol. 21 (17). Date of Electronic Publication: 2020 Aug 24.
Typ publikacji :
Journal Article
MeSH Terms :
Biological Assay/*methods
Drug Evaluation, Preclinical/*methods
Luciferases/*metabolism
Receptors, Dopamine D2/*metabolism
beta-Arrestin 2/*metabolism
Animals ; HEK293 Cells ; Humans ; Kinetics ; Ligands ; Luciferases/analysis ; Luciferases/genetics ; Protein Binding ; Receptors, Dopamine D2/agonists ; Receptors, Dopamine D2/analysis ; beta-Arrestin 2/agonists ; beta-Arrestin 2/analysis
Czasopismo naukowe
Tytuł :
A new brilliantly blue-emitting luciferin-luciferase system from Orfelia fultoni and Keroplatinae (Diptera).
Autorzy :
Viviani VR; Graduate School of Biotechnology and Environmental Monitoring (UFSCar), Federal University of São Carlos (UFSCar), Sorocaba, Brazil. .; Graduate School of Evolutive Genetics and Molecular Biology (UFSCar), São Carlos, Brazil. .
Silva JR; Graduate School of Biotechnology and Environmental Monitoring (UFSCar), Federal University of São Carlos (UFSCar), Sorocaba, Brazil.
Amaral DT; Graduate School of Biotechnology and Environmental Monitoring (UFSCar), Federal University of São Carlos (UFSCar), Sorocaba, Brazil.
Bevilaqua VR; Graduate School of Evolutive Genetics and Molecular Biology (UFSCar), São Carlos, Brazil.
Abdalla FC; Graduate School of Biotechnology and Environmental Monitoring (UFSCar), Federal University of São Carlos (UFSCar), Sorocaba, Brazil.
Branchini BR; Department of Chemistry, Connecticut College, New London, Connecticut, USA.
Johnson CH; Dept. Biological Sciences, Vanderbilt University, Nashville, TN, USA.
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Źródło :
Scientific reports [Sci Rep] 2020 Jun 15; Vol. 10 (1), pp. 9608. Date of Electronic Publication: 2020 Jun 15.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
MeSH Terms :
Diptera/*metabolism
Firefly Luciferin/*metabolism
Luciferases/*metabolism
Animals ; Chromatography, Ion Exchange ; Diptera/enzymology ; Firefly Luciferin/chemistry ; Firefly Luciferin/isolation & purification ; Gene Expression Profiling ; Luciferases/chemistry ; Luciferases/isolation & purification ; Luminescent Measurements ; Mitochondria/enzymology ; Mitochondria/metabolism ; Spectrometry, Fluorescence
Czasopismo naukowe
Tytuł :
Bioluminescent Protein-Inhibitor Pair in the Design of a Molecular Aptamer Beacon Biosensing System.
Autorzy :
Moutsiopoulou A; University of Miami, Leonard M. Miller School of Medicine, Department of Biochemistry and Molecular Biology, Miami, Florida 33136, United States.; University of Miami, Department of Chemistry, Coral Gables, Florida 33146, United States.
Broyles D; University of Miami, Leonard M. Miller School of Medicine, Department of Biochemistry and Molecular Biology, Miami, Florida 33136, United States.
Joda H; University of Miami, Leonard M. Miller School of Medicine, Department of Biochemistry and Molecular Biology, Miami, Florida 33136, United States.
Dikici E; University of Miami, Leonard M. Miller School of Medicine, Department of Biochemistry and Molecular Biology, Miami, Florida 33136, United States.
Kaur A; University of Miami, Leonard M. Miller School of Medicine, Department of Biochemistry and Molecular Biology, Miami, Florida 33136, United States.
Kaifer A; University of Miami, Department of Chemistry, Coral Gables, Florida 33146, United States.
Daunert S; University of Miami, Leonard M. Miller School of Medicine, Department of Biochemistry and Molecular Biology, Miami, Florida 33136, United States.; Dr. John T. Macdonald Foundation Biomedical Nanotechnology Institute of the University of Miami, Miami, Florida 33136, United States.; University of Miami Clinical and Translational Science Institute, Miami, Florida 33136, United States.
Deo SK; University of Miami, Leonard M. Miller School of Medicine, Department of Biochemistry and Molecular Biology, Miami, Florida 33136, United States.; Dr. John T. Macdonald Foundation Biomedical Nanotechnology Institute of the University of Miami, Miami, Florida 33136, United States.
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Źródło :
Analytical chemistry [Anal Chem] 2020 Jun 02; Vol. 92 (11), pp. 7393-7398. Date of Electronic Publication: 2020 May 21.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.
MeSH Terms :
Biosensing Techniques*
Aptamers, Nucleotide/*chemistry
Enzyme Inhibitors/*chemistry
Imidazoles/*chemistry
Luciferases/*chemistry
Luminescent Proteins/*chemistry
Pyrazines/*chemistry
Animals ; Aptamers, Nucleotide/chemical synthesis ; Copepoda/enzymology ; Enzyme Inhibitors/pharmacology ; Imidazoles/pharmacology ; Luciferases/antagonists & inhibitors ; Luciferases/metabolism ; Luminescent Measurements ; Luminescent Proteins/antagonists & inhibitors ; Luminescent Proteins/metabolism ; Models, Molecular ; Molecular Structure ; Pyrazines/pharmacology ; Signal-To-Noise Ratio
Czasopismo naukowe
Tytuł :
In Vivo Optical Reporter-Gene-Based Imaging of Macrophage Infiltration of DNCB-Induced Atopic Dermatitis.
Autorzy :
Lee SB; Korea Institute of Medical Microrobotics (KIMIRo), Gwangju 61011, Korea.
Park H; Laboratory Animal Center, Daegu-Gyeongbuk Medical Innovation Foundation, Daegu 700-721, Korea.; Research Center of Stickus Corporation, Haeundae-gu jaesong-dong 1050-21, Busan 48054, Korea.
Lee JE; Laboratory Animal Center, Daegu-Gyeongbuk Medical Innovation Foundation, Daegu 700-721, Korea.; Department of Biomaterials Science, College of Natural Resources and Life Science/Life and Industry Convergence Research Institute, Pusan National University, Pusan 50463, Korea.
Kim KS; Laboratory Animal Center, Daegu-Gyeongbuk Medical Innovation Foundation, Daegu 700-721, Korea.; College of Veterinary Medicine, Kyungpook National University, Daegu 700-721, Korea.
Jeon YH; Laboratory Animal Center, Daegu-Gyeongbuk Medical Innovation Foundation, Daegu 700-721, Korea.; Leading-Edge Research Center for Drug Discovery and Development for Diabetes and Metabolic Disease, Kyungpook National University Hospital, Daegu 700-721, Korea.
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Źródło :
International journal of molecular sciences [Int J Mol Sci] 2020 Aug 27; Vol. 21 (17). Date of Electronic Publication: 2020 Aug 27.
Typ publikacji :
Journal Article
MeSH Terms :
Dermatitis, Atopic/*metabolism
Dexamethasone/*administration & dosage
Dinitrochlorobenzene/*adverse effects
Luciferases, Firefly/*genetics
Macrophages/*transplantation
Administration, Intravenous ; Animals ; Cell Line ; Dermatitis, Atopic/chemically induced ; Dexamethasone/pharmacology ; Disease Models, Animal ; Female ; Genes, Reporter ; Luciferases, Firefly/metabolism ; Macrophages/drug effects ; Macrophages/metabolism ; Mice ; Mice, Inbred BALB C ; Molecular Imaging ; Optical Imaging ; RAW 264.7 Cells ; Treatment Outcome
Czasopismo naukowe
Tytuł :
Multicomponent Bioluminescence Imaging with a π-Extended Luciferin.
Autorzy :
Yao Z
Zhang BS
Steinhardt RC
Mills JH
Prescher JA
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Źródło :
Journal of the American Chemical Society [J Am Chem Soc] 2020 Aug 19; Vol. 142 (33), pp. 14080-14089. Date of Electronic Publication: 2020 Aug 04.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.
MeSH Terms :
Luminescent Measurements*
Firefly Luciferin/*chemistry
Luciferases/*chemistry
Luminescent Agents/*chemistry
Firefly Luciferin/chemical synthesis ; Luciferases/metabolism ; Luminescence ; Luminescent Agents/chemical synthesis ; Molecular Structure
Czasopismo naukowe
Tytuł :
An Ultrasensitive Bioluminescent Enzyme Immunoassay Based on Nanobody/Nanoluciferase Heptamer Fusion for the Detection of Tetrabromobisphenol A in Sediment.
Autorzy :
Li Z; Department of Entomology and Nematology and UCD Comprehensive Cancer Center, University of California, Davis, California 95616, United States.
Wang Y; Department of Entomology and Nematology and UCD Comprehensive Cancer Center, University of California, Davis, California 95616, United States.; Department of Pesticides Science, School of Resources and Environment, Anhui Agricultural University, No. 130 Changjiang West Road, Hefei 230036, China.
Vasylieva N; Department of Entomology and Nematology and UCD Comprehensive Cancer Center, University of California, Davis, California 95616, United States.
Wan D; Department of Entomology and Nematology and UCD Comprehensive Cancer Center, University of California, Davis, California 95616, United States.
Yin Z; Department of Entomology and Nematology and UCD Comprehensive Cancer Center, University of California, Davis, California 95616, United States.
Dong J; Shenzhen Forward Pharma Co., Ltd., Shenzhen 518057, China.
Hammock BD; Department of Entomology and Nematology and UCD Comprehensive Cancer Center, University of California, Davis, California 95616, United States.
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Źródło :
Analytical chemistry [Anal Chem] 2020 Jul 21; Vol. 92 (14), pp. 10083-10090. Date of Electronic Publication: 2020 Jul 07.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
MeSH Terms :
Environmental Monitoring*
Luminescent Measurements*
Geologic Sediments/*chemistry
Luciferases/*chemistry
Polybrominated Biphenyls/*analysis
Single-Domain Antibodies/*chemistry
Immunoenzyme Techniques ; Luciferases/metabolism
Czasopismo naukowe
Tytuł :
GL261 luciferase-expressing cells elicit an anti-tumor immune response: an evaluation of murine glioma models.
Autorzy :
Sanchez VE; Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, USA.
Lynes JP; Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, USA.
Walbridge S; Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, USA.
Wang X; Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, USA.
Edwards NA; Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, USA.
Nwankwo AK; Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, USA.
Sur HP; Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, USA.
Dominah GA; Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, USA.
Obungu A; Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, USA.
Adamstein N; Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, USA.
Dagur PK; Flow Cytometry Core Facility, National Heart, Lung, and Blood Institute, Bethesda, MD, USA.
Maric D; Flow Cytometry Core Facility, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, USA.
Munasinghe J; Mouse Imaging Facility, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, USA.
Heiss JD; Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, USA.
Nduom EK; Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, USA. .; Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, NIH, Room 3D-20, 10 Center Drive, Bethesda, MD, 20892, USA. .
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Źródło :
Scientific reports [Sci Rep] 2020 Jul 03; Vol. 10 (1), pp. 11003. Date of Electronic Publication: 2020 Jul 03.
Typ publikacji :
Journal Article; Research Support, N.I.H., Intramural
MeSH Terms :
Up-Regulation*
Brain Neoplasms/*metabolism
Cytokines/*metabolism
Glioma/*metabolism
Luciferases/*immunology
Animals ; Cell Line, Tumor ; Cell Survival ; Female ; Gene Expression Regulation, Neoplastic ; Kaplan-Meier Estimate ; Luciferases/genetics ; Mice ; Neoplasm Transplantation ; Proteomics/methods ; Tumor Microenvironment
Czasopismo naukowe
Tytuł :
Development of near-infrared firefly luciferin analogue reacted with wild-type and mutant luciferases.
Autorzy :
Kitada N; Department of Engineering Science, Graduate School of Informatics and Engineering,, The University of Electro-Communications, Chofu, Japan.; Center for Neuroscience and Biomedical Engineering, The University of Electro-Communications, Chofu, Japan.
Saito R; Department of Engineering Science, Graduate School of Informatics and Engineering,, The University of Electro-Communications, Chofu, Japan.; School of Pharmacy, Tokyo University of Pharmacy and Life Science, Tokyo, Japan.
Obata R; Department of Engineering Science, Graduate School of Informatics and Engineering,, The University of Electro-Communications, Chofu, Japan.
Iwano S; Laboratory for Cell Function and Dynamics, Center for Brain Science, Saitama, Japan.
Karube K; Department of Engineering Science, Graduate School of Informatics and Engineering,, The University of Electro-Communications, Chofu, Japan.
Miyawaki A; Laboratory for Cell Function and Dynamics, Center for Brain Science, Saitama, Japan.
Hirano T; Department of Engineering Science, Graduate School of Informatics and Engineering,, The University of Electro-Communications, Chofu, Japan.
Maki SA; Department of Engineering Science, Graduate School of Informatics and Engineering,, The University of Electro-Communications, Chofu, Japan.; Center for Neuroscience and Biomedical Engineering, The University of Electro-Communications, Chofu, Japan.
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Źródło :
Chirality [Chirality] 2020 Jul; Vol. 32 (7), pp. 922-931. Date of Electronic Publication: 2020 May 04.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Firefly Luciferin/*chemistry
Luciferases/*chemistry
Animals ; Firefly Luciferin/analogs & derivatives ; Luciferases/metabolism ; Luminescent Measurements/methods ; Mice ; Stereoisomerism ; Structure-Activity Relationship
Czasopismo naukowe

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