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Wyszukujesz frazę ""Methods in Enzymology"" wg kryterium: JN


Tytuł :
Assessment of insulin proteolysis in rat liver endosomes: its relationship to intracellular insulin signaling.
Autorzy :
Authier F; Service information scientifique et technique (IST) de l'Inserm, Délégation régionale Inserm Paris V, Paris, France. Electronic address: .
Desbuquois B; Inserm U567, Institut Cochin, CNRS UMR 8104, Université Paris-Descartes, Paris, France.
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Źródło :
Methods in enzymology [Methods Enzymol] 2014; Vol. 535, pp. 3-23.
Typ publikacji :
Journal Article
MeSH Terms :
Endosomes/*enzymology
Insulin/*isolation & purification
Liver/*metabolism
Peptide Hydrolases/*metabolism
Amino Acid Sequence ; Animals ; Chemical Precipitation ; Chromatography, High Pressure Liquid ; Chromatography, Reverse-Phase ; Endosomes/metabolism ; Insulin/chemistry ; Insulin/metabolism ; Molecular Sequence Data ; Phosphorylation ; Protease Inhibitors/pharmacology ; Protein Processing, Post-Translational ; Protein Transport ; Proteolysis ; Rats ; Receptor, Insulin/metabolism ; Signal Transduction ; Trichloroacetic Acid/chemistry
Czasopismo naukowe
Tytuł :
Reconstitution of microtubule-dependent organelle transport.
Autorzy :
Barak P; Department of Biological Sciences, Tata Institute of Fundamental Research, Colaba, Mumbai, Maharashtra, India.
Rai A; Department of Biological Sciences, Tata Institute of Fundamental Research, Colaba, Mumbai, Maharashtra, India.
Dubey AK; Department of Biological Sciences, Tata Institute of Fundamental Research, Colaba, Mumbai, Maharashtra, India.
Rai P; Department of Biological Sciences, Tata Institute of Fundamental Research, Colaba, Mumbai, Maharashtra, India.
Mallik R; Department of Biological Sciences, Tata Institute of Fundamental Research, Colaba, Mumbai, Maharashtra, India. Electronic address: .
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Źródło :
Methods in enzymology [Methods Enzymol] 2014; Vol. 540, pp. 231-48.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Dictyostelium/*cytology
Lipids/*analysis
Liver/*metabolism
Microtubules/*metabolism
Organelles/*metabolism
Phagosomes/*metabolism
Animals ; Biological Transport ; Dictyostelium/metabolism ; Lipid Metabolism ; Optical Tweezers ; Rats
Czasopismo naukowe
Tytuł :
Assessment of endurance exercise training in hepatic endoplasmic reticulum stress.
Autorzy :
Chapados NA; Institut de Recherche de l'Hôpital Montfort/Montfort Hospital, Research Institute, Ottawa, Ontario, Canada.
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Źródło :
Methods in enzymology [Methods Enzymol] 2011; Vol. 489, pp. 97-107.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Stress, Physiological*
Endoplasmic Reticulum/*physiology
Liver/*metabolism
Physical Conditioning, Animal/*physiology
Animals ; Dietary Fats/administration & dosage ; Oxygen Consumption ; Physical Conditioning, Animal/methods ; Physical Endurance ; Rats
Czasopismo naukowe
Tytuł :
The impact of ions on allosteric functions in human liver pyruvate kinase.
Autorzy :
Fenton AW; Department of Biochemistry and Molecular Biology, The University of Kansas Medical Center, Kansas City, Kansas, USA.
Alontaga AY
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Źródło :
Methods in enzymology [Methods Enzymol] 2009; Vol. 466, pp. 83-107. Date of Electronic Publication: 2009 Nov 13.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural
MeSH Terms :
Ions/*metabolism
Liver/*enzymology
Pyruvate Kinase/*metabolism
Allosteric Regulation ; Biochemistry/methods ; Buffers ; Humans ; Phosphoenolpyruvate/metabolism
Czasopismo naukowe
Tytuł :
Analyzing macroautophagy in hepatocytes and the liver.
Autorzy :
Ding WX; Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA.
Yin XM
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Źródło :
Methods in enzymology [Methods Enzymol] 2009; Vol. 453, pp. 397-416.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
MeSH Terms :
Autophagy/*physiology
Hepatocytes/*metabolism
Liver/*metabolism
Animals ; Blotting, Western ; Cells, Cultured ; Hepatocytes/ultrastructure ; Liver/ultrastructure ; Mice ; Microscopy, Electron ; Microtubule-Associated Proteins/metabolism
Czasopismo naukowe
Tytuł :
Methods for inducing and monitoring liver autophagy relative to aging and antiaging caloric restriction in rats.
Autorzy :
Donati A; Centro di Ricerca Interdipartimentale di Biologia e Patologia dell'Invecchiamento, Università di Pisa, Pisa, Italy.
Cavallini G
Bergamini E
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Źródło :
Methods in enzymology [Methods Enzymol] 2009; Vol. 452, pp. 441-55.
Typ publikacji :
Journal Article
MeSH Terms :
Caloric Restriction*
Aging/*physiology
Autophagy/*physiology
Liver/*metabolism
Animals ; Hepatocytes/metabolism ; Rats ; Rats, Inbred F344 ; Rats, Sprague-Dawley ; Rats, Wistar
Czasopismo naukowe
Tytuł :
Osmosensing by integrins in rat liver.
Autorzy :
Schliess F; Clinic for Gastroenterology, Hepatology, and Infectiology, Heinrich-Heine-University, Düsseldorf, Germany.
Häussinger D
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Źródło :
Methods in enzymology [Methods Enzymol] 2007; Vol. 428, pp. 129-44.
Typ publikacji :
Journal Article; Review
MeSH Terms :
Integrins/*physiology
Liver/*physiology
Mechanotransduction, Cellular/*physiology
Signal Transduction/*physiology
Animals ; Autophagy/physiology ; Bile Acids and Salts/metabolism ; Cell Size ; In Vitro Techniques ; Insulin/physiology ; Osmolar Concentration ; Peptide Hydrolases/metabolism ; Perfusion ; Rats
Czasopismo naukowe
Tytuł :
Osmoregulation of bile formation.
Autorzy :
Kubitz R; Clinic of Gastroenterology, Hepatology and Infectiology, Heinrich-Heine-University Düsseldorf, Düsseldorf, Germany.
Häussinger D
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Źródło :
Methods in enzymology [Methods Enzymol] 2007; Vol. 428, pp. 313-24.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't; Review
MeSH Terms :
Bile/*metabolism
Liver/*physiology
Water-Electrolyte Balance/*physiology
Animals ; Bile Acids and Salts/metabolism ; Bile Canaliculi/physiology ; Humans ; Integrins/physiology ; Osmosis ; Signal Transduction/physiology
Czasopismo naukowe
Tytuł :
Nitric oxide production by primary liver cells isolated from amino acid diet-fed rats.
Autorzy :
Kotake Y; Oklahoma Medical Research Foundation, Free Radical Biology and Aging Research Program, Oklahoma City, OK 73104, USA.
Kishida H
Nakae D
Floyd RA
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Źródło :
Methods in enzymology [Methods Enzymol] 2005; Vol. 396, pp. 535-41.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural
MeSH Terms :
Diet*
Amino Acids/*administration & dosage
Liver/*metabolism
Nitric Oxide/*biosynthesis
Animals ; Cells, Cultured ; Flow Cytometry ; Liver/cytology ; Male ; Rats ; Rats, Wistar
Czasopismo naukowe
Tytuł :
Substrate specificity of human hepatic udp-glucuronosyltransferases.
Autorzy :
Burchell B; Department of Molecular and Cellular Pathology, Ninewells Hospital and Medical School, University of Dundee, Scotland, United Kingdom.
Lockley DJ
Staines A
Uesawa Y
Coughtrie MW
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Źródło :
Methods in enzymology [Methods Enzymol] 2005; Vol. 400, pp. 46-57.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Glucuronides/*chemistry
Glucuronosyltransferase/*chemistry
Liver/*enzymology
Gas Chromatography-Mass Spectrometry ; Glucuronides/metabolism ; Glucuronosyltransferase/classification ; Glucuronosyltransferase/genetics ; Humans ; Molecular Structure ; Substrate Specificity
Czasopismo naukowe
Tytuł :
Oxygen-dependent regulation of hepatic glucose metabolism.
Autorzy :
Kietzmann T; Institut fur Biochemie und Molekulare Zellbiologie, Universitat Gottingen, Germany.
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Źródło :
Methods in enzymology [Methods Enzymol] 2004; Vol. 381, pp. 357-76.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Glucose/*metabolism
Liver/*metabolism
Oxygen/*metabolism
Animals ; Genes, Reporter ; Gluconeogenesis/physiology ; Glycogen/metabolism ; Glycolysis/physiology ; Hepatocytes/metabolism ; Male ; Rats ; Rats, Wistar ; Transcription Factors/metabolism ; Transcription, Genetic/physiology ; Transfection/methods
Czasopismo naukowe
Tytuł :
Biodistribution and uptake of liposomes in vivo.
Autorzy :
Kamps JA; Department of Cell Biology, Groningen University Institute for Drug Exploration, The Netherlands.
Scherphof GL
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Źródło :
Methods in enzymology [Methods Enzymol] 2004; Vol. 387, pp. 257-66.
Typ publikacji :
Journal Article
MeSH Terms :
Liposomes/*pharmacokinetics
Liver/*metabolism
Animals ; Cell Separation/methods ; Collagenases ; Drug Carriers/pharmacokinetics ; Drug Delivery Systems/methods ; Endothelial Cells/metabolism ; Hepatocytes/metabolism ; Kupffer Cells/metabolism ; Mice ; Models, Animal ; Rats ; Tissue Distribution
Czasopismo naukowe
Tytuł :
Detection of protein histidine phosphatase in vertebrates.
Autorzy :
Klumpp S; Institut für Pharmazeutische & Medizinische Chemie, Westfälische Wilhelms-Universität, Hittorfstrasse 58-62, 48149 Münster, Germany.
Hermesmeier J
Krieglstein J
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Źródło :
Methods in enzymology [Methods Enzymol] 2003; Vol. 366, pp. 56-64.
Typ publikacji :
Journal Article
MeSH Terms :
Histidine/*analogs & derivatives
Hydrolases/*metabolism
Liver/*enzymology
Animals ; Bacterial Proteins/metabolism ; Chromatography, Affinity/methods ; Chromatography, Gel/methods ; Chromatography, Ion Exchange/methods ; Histidine/metabolism ; Hydrolases/analysis ; Hydrolases/isolation & purification ; Indicators and Reagents ; Kinetics ; Membrane Proteins/metabolism ; Methyl-Accepting Chemotaxis Proteins ; Phosphorylation ; Substrate Specificity
Czasopismo naukowe
Tytuł :
Differentiation of mouse embryonic stem cells into pancreatic and hepatic cells.
Autorzy :
Kania G; In Vitro Differentiation Group, Institute of Plant Genetics and Crop Plant Research, Corrensstr. 3, Gatersleben, D-06466, Germany.
Blyszczuk P
Czyz J
Navarrete-Santos A
Wobus AM
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Źródło :
Methods in enzymology [Methods Enzymol] 2003; Vol. 365, pp. 287-303.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Nerve Tissue Proteins*
Cell Culture Techniques/*methods
Liver/*cytology
Pancreas/*cytology
Stem Cells/*cytology
Albumins/metabolism ; Animals ; Cell Differentiation ; Enzyme-Linked Immunosorbent Assay ; Flow Cytometry ; Immunohistochemistry ; Insulin/metabolism ; Intermediate Filament Proteins/metabolism ; Liver/metabolism ; Mice ; Microscopy, Fluorescence ; Microscopy, Phase-Contrast ; Nestin ; Pancreas/metabolism ; Phenotype ; Reverse Transcriptase Polymerase Chain Reaction ; Signal Transduction ; Time Factors
Czasopismo naukowe
Tytuł :
Proteomic analysis of rodent hepatic responses to peroxisome proliferators.
Autorzy :
Macdonald N; Central Toxicology Laboratory, Syngenta, Macclesfield SK10 4TJ, United Kingdom.
Roberts R
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Źródło :
Methods in enzymology [Methods Enzymol] 2002; Vol. 357, pp. 249-58.
Typ publikacji :
Journal Article
MeSH Terms :
Protein Array Analysis*
Liver/*drug effects
Peroxisome Proliferators/*pharmacology
Proteomics/*methods
Animals ; Cytochrome P-450 Enzyme System/analysis ; Electrophoresis, Gel, Two-Dimensional ; Humans ; Liver/metabolism ; Mice ; Rats ; Receptors, Cytoplasmic and Nuclear/metabolism ; Transcription Factors/metabolism
Czasopismo naukowe
Tytuł :
Direct expression of fluorescent protein-tagged nuclear receptor CAR in mouse liver.
Autorzy :
Sueyoshi T; Laboratory of Reproductive and Developmental Toxicology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA.
Moore R
Pascussi JM
Negishi M
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Źródło :
Methods in enzymology [Methods Enzymol] 2002; Vol. 357, pp. 205-13.
Typ publikacji :
Journal Article
MeSH Terms :
Gene Expression Regulation*/drug effects
Liver/*metabolism
Luminescent Proteins/*metabolism
Receptors, Cytoplasmic and Nuclear/*metabolism
Transcription Factors/*metabolism
Animals ; Chlorpromazine/metabolism ; Chlorpromazine/pharmacology ; Cytochrome P-450 Enzyme System/genetics ; Green Fluorescent Proteins ; Hepatocytes/cytology ; Hepatocytes/metabolism ; Indicators and Reagents/metabolism ; Liver/chemistry ; Liver/cytology ; Luminescent Proteins/genetics ; Mice ; Plasmids/genetics ; Plasmids/metabolism ; Receptors, Cytoplasmic and Nuclear/genetics ; Receptors, Glucocorticoid/genetics ; Receptors, Glucocorticoid/metabolism ; Recombinant Fusion Proteins/genetics ; Recombinant Fusion Proteins/metabolism ; Transcription Factors/genetics
Czasopismo naukowe
Tytuł :
Purification and properties of rat liver Sec23-Sec24 complex.
Autorzy :
Weissman JT; Department of Cell Biology, Scripps Research Institute, La Jolla, California 92037, USA.
Aridor M
Balch WE
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Źródło :
Methods in enzymology [Methods Enzymol] 2001; Vol. 329, pp. 431-8.
Typ publikacji :
Journal Article
MeSH Terms :
Saccharomyces cerevisiae Proteins*
Liver/*chemistry
Membrane Proteins/*isolation & purification
Membrane Proteins/*metabolism
Proteins/*isolation & purification
Proteins/*metabolism
Animals ; COP-Coated Vesicles/metabolism ; Chromatography, Gel ; Chromatography, Ion Exchange ; Electrophoresis, Polyacrylamide Gel ; Endoplasmic Reticulum/metabolism ; Enzyme Activation ; Kinetics ; Macromolecular Substances ; Male ; Monomeric GTP-Binding Proteins/metabolism ; Rats ; Rats, Sprague-Dawley ; Vesicular Transport Proteins
Czasopismo naukowe
Tytuł :
Liver perchloric acid-soluble ribonuclease.
Autorzy :
Sawasaki T; Department of Applied Chemistry, Faculty of Engineering, Ehime University, Matsuyama 790-8577, Japan.
Oka T
Endo Y
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Źródło :
Methods in enzymology [Methods Enzymol] 2001; Vol. 342, pp. 44-54.
Typ publikacji :
Journal Article
MeSH Terms :
Heat-Shock Proteins/*metabolism
Liver/*drug effects
Perchlorates/*pharmacology
Ribonucleases/*metabolism
Amino Acid Sequence ; Animals ; Base Sequence ; DNA, Complementary ; Heat-Shock Proteins/chemistry ; Heat-Shock Proteins/genetics ; Heat-Shock Proteins/isolation & purification ; Hydrolysis ; Liver/enzymology ; Male ; Molecular Sequence Data ; RNA, Ribosomal, 5S/metabolism ; Rats ; Rats, Wistar ; Ribonucleases/chemistry ; Ribonucleases/genetics ; Ribonucleases/isolation & purification ; Solubility ; Substrate Specificity
Czasopismo naukowe
Tytuł :
Antisense oligonucleotide therapy of hepadnavirus infection.
Autorzy :
Offensperger WB; Department of Medicine II, University of Freiburg, Germany.
Thoma C
Moradpour D
von Weizsäcker F
Offensperger S
Blum HE
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Źródło :
Methods in enzymology [Methods Enzymol] 2000; Vol. 314, pp. 524-36.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Hepadnaviridae Infections/*drug therapy
Hepatitis B Virus, Duck/*drug effects
Liver/*drug effects
Oligodeoxyribonucleotides, Antisense/*therapeutic use
Thionucleotides/*therapeutic use
Animals ; Ducks ; Hepatitis B Virus, Duck/genetics ; Liver/cytology ; Virus Replication/drug effects
Czasopismo naukowe
Tytuł :
Purification, properties, and sequencing of aminoisobutyrate aminotransferases from rat liver.
Autorzy :
Tamaki N; Laboratory of Nutritional Chemistry, Faculty of Nutrition, Kobe Gakuin University, Japan.
Sakata SF
Matsuda K
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Źródło :
Methods in enzymology [Methods Enzymol] 2000; Vol. 324, pp. 376-89.
Typ publikacji :
Journal Article
MeSH Terms :
Liver/*enzymology
Transaminases/*isolation & purification
Amino Acid Sequence ; Animals ; Base Sequence ; Chromatography, Liquid ; DNA Primers ; Molecular Sequence Data ; Rats ; Sequence Homology, Amino Acid ; Transaminases/chemistry ; Transaminases/metabolism
Czasopismo naukowe
Tytuł :
Regulation of branched-chain alpha-keto acid dehydrogenase kinase gene expression by glucocorticoids in hepatoma cells and rat liver.
Autorzy :
Huang YS; Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas 75390-9038, USA.
Chuang DT
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Źródło :
Methods in enzymology [Methods Enzymol] 2000; Vol. 324, pp. 498-511.
Typ publikacji :
Journal Article
MeSH Terms :
Gene Expression Regulation, Enzymologic*
Dexamethasone/*pharmacology
Liver/*drug effects
Liver Neoplasms, Experimental/*enzymology
Protein Kinases/*genetics
Animals ; Liver/enzymology ; Models, Animal ; Promoter Regions, Genetic ; Rats ; Tumor Cells, Cultured
Czasopismo naukowe
Tytuł :
Targeted delivery of antisense oligonucleotides to parenchymal liver cells in vivo.
Autorzy :
Biessen EA; Division of Biopharmaceutics, Leiden/Amsterdam Center for Drug Research, Leiden University, The Netherlands.
Vietsch H
Rump ET
Fluiter K
Bijsterbosch MK
van Berkel TJ
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Źródło :
Methods in enzymology [Methods Enzymol] 2000; Vol. 314, pp. 324-42.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Membrane Proteins*
Receptors, Lipoprotein*
Drug Delivery Systems/*methods
Liver/*drug effects
Oligodeoxyribonucleotides, Antisense/*administration & dosage
Receptors, Immunologic/*metabolism
Animals ; Asialoglycoproteins/metabolism ; Biological Availability ; Drug Carriers ; Drug Stability ; Glycopeptides ; Liver/cytology ; Oligodeoxyribonucleotides, Antisense/chemistry ; Oligodeoxyribonucleotides, Antisense/pharmacokinetics ; Orosomucoid/metabolism ; Rats ; Receptors, Scavenger ; Scavenger Receptors, Class B
Czasopismo naukowe
Tytuł :
Assessment of physiological interaction between vitamin E and vitamin C.
Autorzy :
Glascott PA Jr; Rhône-Poulenc Rorer Research and Development, Drug Safety, Collegeville, Pennsylvania 19426-0994, USA.
Farber JL
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Źródło :
Methods in enzymology [Methods Enzymol] 1999; Vol. 300, pp. 78-88.
Typ publikacji :
Journal Article
MeSH Terms :
Antioxidants/*metabolism
Ascorbic Acid/*metabolism
Liver/*metabolism
Vitamin E/*metabolism
Animals ; Ascorbic Acid/analysis ; Cells, Cultured ; Liver/cytology ; Male ; Oxidative Stress ; Rats ; Rats, Sprague-Dawley ; Thiobarbiturates/analysis ; Vitamin E/analysis
Czasopismo naukowe
Tytuł :
Ascorbic acid recycling in rat hepatocytes as measurement of antioxidant capacity: decline with age.
Autorzy :
Lykkesfeldt J; Department of Molecular and Cell Biology, University of California, Berkeley 94720-3202, USA.
Ames BN
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Źródło :
Methods in enzymology [Methods Enzymol] 1999; Vol. 299, pp. 83-8.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.
MeSH Terms :
Aging/*metabolism
Ascorbic Acid/*metabolism
Liver/*metabolism
Oxidative Stress/*physiology
Animals ; Cell Separation ; Liver/cytology ; Male ; Rats ; Rats, Inbred F344
Czasopismo naukowe
Tytuł :
Purification and characterization of cellular carotenoid-binding protein from mammalian liver.
Autorzy :
Lakshman MR; Lipid Research Laboratory, DVA Medical Center, Washington, DC, USA.
Rao MN
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Źródło :
Methods in enzymology [Methods Enzymol] 1999; Vol. 299, pp. 441-56.
Typ publikacji :
Journal Article; Research Support, U.S. Gov't, P.H.S.
MeSH Terms :
Ferrets*
Carrier Proteins/*isolation & purification
Liver/*chemistry
beta Carotene/*metabolism
Animals ; Binding, Competitive ; Carotenoids/metabolism ; Carrier Proteins/metabolism ; Chromatography, Affinity/methods ; Male ; Vitamin A/metabolism
Czasopismo naukowe
Tytuł :
Assay of phylloquinone and menaquinones in human liver.
Autorzy :
Usui Y; Department of Surgery, Tazuke Kofukai Medical Research Institute, Osaka, Japan.
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Źródło :
Methods in enzymology [Methods Enzymol] 1997; Vol. 282, pp. 438-46.
Typ publikacji :
Journal Article
MeSH Terms :
Hepatitis, Chronic/*metabolism
Liver/*chemistry
Liver Cirrhosis/*metabolism
Vitamin K/*analogs & derivatives
Vitamin K/*analysis
Vitamin K 1/*analysis
Chromatography, High Pressure Liquid/instrumentation ; Chromatography, High Pressure Liquid/methods ; Humans ; Indicators and Reagents ; Reference Values ; Vitamin K 1/analogs & derivatives
Czasopismo naukowe
Tytuł :
Methionine synthase from pig liver.
Autorzy :
Banerjee R; Department of Biochemistry, University of Nebraska, Lincoln 68588-0664, USA.
Chen Z
Gulati S
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Źródło :
Methods in enzymology [Methods Enzymol] 1997; Vol. 281, pp. 189-96.
Typ publikacji :
Journal Article; Research Support, U.S. Gov't, P.H.S.
MeSH Terms :
5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase/*isolation & purification
5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase/*metabolism
Liver/*enzymology
5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase/chemistry ; Animals ; Carbon Radioisotopes ; Chromatography ; Chromatography, Gel ; Homocysteine/metabolism ; Hydrogen-Ion Concentration ; Methionine/biosynthesis ; Molecular Weight ; Spectrophotometry ; Swine ; Tetrahydrofolates/metabolism
Czasopismo naukowe
Tytuł :
Purification and characterization of glutathione-dependent dehydroascorbate reductase from rat liver.
Autorzy :
Maellaro E; Istituto di Patologia Generale, Università di Siena, Italy.
Del Bello B
Sugherini L
Comporti M
Casini AF
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Źródło :
Methods in enzymology [Methods Enzymol] 1997; Vol. 279, pp. 30-5.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't; Review
MeSH Terms :
Liver/*enzymology
Oxidoreductases/*isolation & purification
Animals ; Biological Assay ; Rats
Czasopismo naukowe
Tytuł :
Purification and characterization of aquacobalamin reductases from mammals.
Autorzy :
Watanabe F; Department of Food and Nutrition, Kochi Women's University, Japan.
Nakano Y
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Źródło :
Methods in enzymology [Methods Enzymol] 1997; Vol. 281, pp. 295-305.
Typ publikacji :
Journal Article
MeSH Terms :
Liver/*enzymology
NADH, NADPH Oxidoreductases/*isolation & purification
NADH, NADPH Oxidoreductases/*metabolism
Animals ; Cytochrome Reductases/metabolism ; Cytochromes b5/metabolism ; Electron Transport ; Hydrogen-Ion Concentration ; Kinetics ; Microsomes, Liver/enzymology ; Mitochondria, Liver/enzymology ; NADP/metabolism ; Oxidation-Reduction ; Rats ; Temperature
Czasopismo naukowe
Tytuł :
Purification and properties of FAD synthetase from liver.
Autorzy :
McCormick DB; Department of Biochemistry, Rollins Research Center, Emory University, Atlanta, Georgia 30322, USA.
Oka M
Bowers-Komro DM
Yamada Y
Hartman HA
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Źródło :
Methods in enzymology [Methods Enzymol] 1997; Vol. 280, pp. 407-13.
Typ publikacji :
Journal Article
MeSH Terms :
Liver/*enzymology
Nucleotidyltransferases/*isolation & purification
Adenosine Triphosphate/metabolism ; Animals ; Apoenzymes ; Cations/pharmacology ; Coenzymes ; D-Amino-Acid Oxidase/metabolism ; Enzyme Stability ; Flavin Mononucleotide/metabolism ; Hydrogen-Ion Concentration ; Magnesium/metabolism ; Molecular Weight ; Nucleotidyltransferases/drug effects ; Nucleotidyltransferases/metabolism ; Rats ; Substrate Specificity
Czasopismo naukowe
Tytuł :
Radiation inactivation studies of hepatic cholesteryl ester hydrolases.
Autorzy :
Harrison EH; Department of Biochemistry, MCP-Hahnemann School of Medicine, Philadelphia, Pennsylvania 19129, USA.
Kempner ES
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Źródło :
Methods in enzymology [Methods Enzymol] 1997; Vol. 286, pp. 116-26.
Typ publikacji :
Journal Article
MeSH Terms :
Liver/*enzymology
Sterol Esterase/*antagonists & inhibitors
Sterol Esterase/*radiation effects
Animals ; Carboxylesterase ; Carboxylic Ester Hydrolases/antagonists & inhibitors ; Carboxylic Ester Hydrolases/radiation effects ; Enzyme Stability ; Hydrogen-Ion Concentration ; Lipase/analysis ; Lipase/radiation effects ; Microsomes, Liver/enzymology ; Rats
Czasopismo naukowe
Tytuł :
Influence of various signal peptides on secretion of mammalian acidic lipases in baculovirus-insect cell system.
Autorzy :
Dupuis L; Laboratoire de Lipolyse Enzymatique, UPR-9025, Marseille, France.
Canaan S
Rivière M
Wicker-Planquart C
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Źródło :
Methods in enzymology [Methods Enzymol] 1997; Vol. 284, pp. 261-72.
Typ publikacji :
Journal Article
MeSH Terms :
Gastric Mucosa/*enzymology
Lipase/*biosynthesis
Liver/*enzymology
Protein Sorting Signals/*metabolism
Amino Acid Sequence ; Animals ; Baculoviridae ; Base Sequence ; Cell Line ; DNA Primers ; Humans ; Lipase/chemistry ; Lipase/genetics ; Lysosomes/enzymology ; Mammals ; Molecular Sequence Data ; Polymerase Chain Reaction ; Recombinant Fusion Proteins/biosynthesis ; Recombinant Fusion Proteins/chemistry ; Restriction Mapping ; Spodoptera ; Transfection/methods
Czasopismo naukowe
Tytuł :
Human 5,10-methenyltetrahydrofolate synthetase.
Autorzy :
Jolivet J; Centre de Recherche, Centre Hospitalier de L'Université de Montréal, Montreal, Quebec, Canada.
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Źródło :
Methods in enzymology [Methods Enzymol] 1997; Vol. 281, pp. 162-70.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Carbon-Nitrogen Ligases*
Ligases/*isolation & purification
Ligases/*metabolism
Liver/*enzymology
Mitochondria, Liver/*enzymology
Blotting, Southern ; Cloning, Molecular ; Cytosol/enzymology ; DNA Primers ; Enzyme Inhibitors/pharmacology ; Escherichia coli/genetics ; Humans ; Kinetics ; Leucovorin/metabolism ; Ligases/chemistry ; Molecular Sequence Data ; RNA, Messenger/metabolism ; Recombinant Proteins/chemistry ; Recombinant Proteins/isolation & purification ; Recombinant Proteins/metabolism ; Sequence Analysis, DNA ; Spectrophotometry ; Tetrahydrofolates/metabolism ; Tetrahydrofolates/pharmacology
Czasopismo naukowe
Tytuł :
Hepatic lipase: high-level expression and subunit structure determination.
Autorzy :
Hill JS; Lipid Research Laboratory, West Los Angeles VA Medical Center, California 90073, USA.
Davis RC
Yang D
Schotz MC
Wong H
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Źródło :
Methods in enzymology [Methods Enzymol] 1997; Vol. 284, pp. 232-46.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.
MeSH Terms :
Lipase/*biosynthesis
Lipase/*chemistry
Liver/*enzymology
Animals ; CHO Cells ; Chromatography ; Chromatography, Affinity ; Chromatography, Ion Exchange ; Cloning, Molecular ; Cricetinae ; Durapatite ; Humans ; Indicators and Reagents ; Kinetics ; Lipase/isolation & purification ; Macromolecular Substances ; Recombinant Proteins/biosynthesis ; Recombinant Proteins/chemistry ; Recombinant Proteins/isolation & purification ; Restriction Mapping ; Transfection/methods
Czasopismo naukowe
Tytuł :
Sandwich immunoassay for measurement of human hepatic lipase.
Autorzy :
Bensadoun A; Division of Nutritional Sciences, Cornell University, Ithaca, New York 14853, USA.
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Źródło :
Methods in enzymology [Methods Enzymol] 1996; Vol. 263, pp. 333-8.
Typ publikacji :
Journal Article
MeSH Terms :
Enzyme-Linked Immunosorbent Assay/*methods
Lipase/*metabolism
Liver/*enzymology
Antibodies, Monoclonal/immunology ; Humans ; Lipase/immunology ; Lipase/isolation & purification
Czasopismo naukowe
Tytuł :
Trimethadione: metabolism and assessment of hepatic drug-oxidizing capacity.
Autorzy :
Tanaka E; Institute of Community Medicine, University of Tsukuba, Japan.
Funae Y
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Źródło :
Methods in enzymology [Methods Enzymol] 1996; Vol. 272, pp. 163-9.
Typ publikacji :
Journal Article
MeSH Terms :
Liver/*metabolism
Trimethadione/*metabolism
Animals ; Anticonvulsants/analysis ; Anticonvulsants/metabolism ; Anticonvulsants/pharmacokinetics ; Chromatography, Gas ; Dimethadione/analysis ; Dimethadione/metabolism ; Humans ; In Vitro Techniques ; Liver Diseases/metabolism ; Male ; Microsomes, Liver/metabolism ; Oxidation-Reduction ; Rats ; Rats, Sprague-Dawley ; Trimethadione/analysis ; Trimethadione/pharmacokinetics
Czasopismo naukowe
Tytuł :
13Bovine mitochondrial ribosomes.
Autorzy :
O'Brien TW; Department of Biochemistry and Molecular Biology, University of Florida College of Medicine, Gainesville 32610, USA.
Denslow ND
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Źródło :
Methods in enzymology [Methods Enzymol] 1996; Vol. 264, pp. 237-48.
Typ publikacji :
Journal Article
MeSH Terms :
Protein Biosynthesis*
Liver/*ultrastructure
Mitochondria, Liver/*ultrastructure
Ribosomes/*ultrastructure
Amino Acyl-tRNA Synthetases/metabolism ; Animals ; Cattle ; Cell Fractionation/methods ; Centrifugation, Density Gradient/methods ; Centrifugation, Zonal/methods ; Cesium ; Chlorides ; DEAE-Cellulose ; Indicators and Reagents ; Leucine/metabolism ; Liver/metabolism ; Mitochondria, Liver/metabolism ; Peptidyl Transferases/metabolism ; RNA, Transfer, Leu/metabolism ; Radioisotope Dilution Technique ; Ribosomes/metabolism ; Sucrose ; Tritium ; Ultrafiltration/methods
Czasopismo naukowe
Tytuł :
Use of human hepatocytes to study P450 gene induction.
Autorzy :
Strom SC; Department of Pathology, University of Pittsburgh, Pennsylvania 15261, USA.
Pisarov LA
Dorko K
Thompson MT
Schuetz JD
Schuetz EG
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Źródło :
Methods in enzymology [Methods Enzymol] 1996; Vol. 272, pp. 388-401.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.
MeSH Terms :
Gene Expression Regulation, Enzymologic*
Cytochrome P-450 Enzyme System/*genetics
Liver/*enzymology
Adolescent ; Adult ; Cell Separation ; Child ; Child, Preschool ; Cytochrome P-450 Enzyme System/biosynthesis ; Enzyme Induction ; Humans ; In Vitro Techniques ; Infant ; Infant, Newborn ; Liver/cytology ; Middle Aged ; Tissue Donors ; Transcriptional Activation
Czasopismo naukowe
Tytuł :
Chlorzoxazone: an in vitro and in vivo substrate probe for liver CYP2E1.
Autorzy :
Lucas D; Labs Biochimie-Nutrition, Faculté de Médecine, Brest, France.
Menez JF
Berthou F
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Źródło :
Methods in enzymology [Methods Enzymol] 1996; Vol. 272, pp. 115-23.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Chlorzoxazone/*metabolism
Cytochrome P-450 CYP2E1/*metabolism
Liver/*enzymology
Chlorzoxazone/analogs & derivatives ; Cytochrome P-450 CYP2E1/analysis ; Cytochrome P-450 CYP2E1/genetics ; Humans ; Hydroxylation ; In Vitro Techniques ; Microsomes, Liver/enzymology ; Molecular Probes ; Muscle Relaxants, Central/metabolism ; Phenotype ; Recombinant Proteins/genetics ; Recombinant Proteins/metabolism ; Substrate Specificity
Czasopismo naukowe
Tytuł :
Purification of RNA polymerase II general transcription factors from rat liver.
Autorzy :
Conaway RC; Program in Molecular and Cell Biology, Oklahoma Medical Research Foundation, Oklahoma City 73104, USA.
Reines D
Garrett KP
Powell W
Conaway JW
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Źródło :
Methods in enzymology [Methods Enzymol] 1996; Vol. 273, pp. 194-207.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.; Review
MeSH Terms :
Transcription Factors, General*
Transcription Factors, TFII*
Transcriptional Elongation Factors*
Liver/*chemistry
RNA Polymerase II/*metabolism
Transcription Factors/*isolation & purification
Animals ; Cell Fractionation/methods ; Cell Nucleus/chemistry ; Cytoplasm/chemistry ; Elongin ; Male ; Precipitin Tests ; Rats ; Rats, Sprague-Dawley ; Transcription Factor TFIIA ; Transcription Factor TFIIB ; Transcription Factor TFIID ; Transcription Factor TFIIH ; Transcription Factors/metabolism ; Transcription, Genetic
Czasopismo naukowe
Tytuł :
Protein disulfide-isomerase.
Autorzy :
Freedman RB; Research School of Biosciences, University of Kent, Canterbury, United Kingdom.
Hawkins HC
McLaughlin SH
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Źródło :
Methods in enzymology [Methods Enzymol] 1995; Vol. 251, pp. 397-406.
Typ publikacji :
Journal Article
MeSH Terms :
Isomerases/*isolation & purification
Isomerases/*metabolism
Liver/*enzymology
Animals ; Cattle ; Chromatography, High Pressure Liquid/methods ; Chromatography, Ion Exchange/methods ; Dithiothreitol/pharmacology ; Electrophoresis, Polyacrylamide Gel/methods ; Hot Temperature ; Indicators and Reagents ; Isomerases/chemistry ; Molecular Weight ; Pancreas/enzymology ; Protein Disulfide-Isomerases ; Ribonucleases/metabolism ; Substrate Specificity
Czasopismo naukowe

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