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Wyszukujesz frazę ""Molecular evolution"" wg kryterium: Temat


Tytuł :
Adaptive laboratory evolution of the fast-growing cyanobacterium Synechococcus elongatus PCC 11801 for improved solvent tolerance.
Autorzy :
Srivastava V; Department of Chemical Engineering, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India.
Amanna R; Department of Chemical Engineering, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India.
Rowden SJL; Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1QW, UK.
Sengupta S; Department of Chemical Engineering, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India; DBT-Pan IIT Center for Bioenergy, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India.
Madhu S; Department of Chemical Engineering, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India.
Howe CJ; Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1QW, UK.
Wangikar PP; Department of Chemical Engineering, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India; DBT-Pan IIT Center for Bioenergy, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India; Wadhwani Research Center for Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India. Electronic address: .
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Źródło :
Journal of bioscience and bioengineering [J Biosci Bioeng] 2021 May; Vol. 131 (5), pp. 491-500. Date of Electronic Publication: 2021 Feb 18.
Typ publikacji :
Journal Article
MeSH Terms :
Directed Molecular Evolution*
Solvents/*pharmacology
Synechococcus/*drug effects
Synechococcus/*genetics
Alcohols/pharmacology ; Biofuels ; Carbon Dioxide/metabolism ; Photosynthesis/drug effects ; Synechococcus/metabolism
SCR Organism :
Synechococcus elongatus
Czasopismo naukowe
Tytuł :
Design of novel enzyme biocatalysts for industrial bioprocess: Harnessing the power of protein engineering, high throughput screening and synthetic biology.
Autorzy :
Madhavan A; Rajiv Gandhi Centre for Biotechnology, Trivandrum 695 014, India.
Arun KB; Rajiv Gandhi Centre for Biotechnology, Trivandrum 695 014, India.
Binod P; Microbial Processes and Technology Division, CSIR-National Institute for Interdisciplinary Science and Technology (CSIR-NIIST), Trivandrum 695 019, India.
Sirohi R; The Center for Energy and Environmental Sustainability, Lucknow 226 010, Uttar Pradesh, India.
Tarafdar A; Division of Livestock Production and Management, ICAR - Indian Veterinary Research Institute, Izatnagar, Bareilly 243 122, Uttar Pradesh, India.
Reshmy R; Post Graduate and Research Department of Chemistry, Bishop Moore College, Mavelikara 690 110, Kerala, India.
Kumar Awasthi M; College of Natural Resources and Environment, North West A & F University, Yangling, Shaanxi 712 100, China.
Sindhu R; Microbial Processes and Technology Division, CSIR-National Institute for Interdisciplinary Science and Technology (CSIR-NIIST), Trivandrum 695 019, India. Electronic address: .
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Źródło :
Bioresource technology [Bioresour Technol] 2021 Apr; Vol. 325, pp. 124617. Date of Electronic Publication: 2020 Dec 26.
Typ publikacji :
Journal Article; Review
MeSH Terms :
Directed Molecular Evolution*
Synthetic Biology*
Biocatalysis ; Enzymes/metabolism ; High-Throughput Screening Assays ; Protein Engineering
Czasopismo naukowe
Tytuł :
A High-Throughput Screening System Based on Fluorescence-Activated Cell Sorting for the Directed Evolution of Chitinase A.
Autorzy :
Menghiu G; Institute for Biology VII, Molecular Biotechnology, RWTH Aachen University, Worringerweg 1, 52074 Aachen, Germany.; Advanced Environmental Research Laboratories, Department of Biology-Chemistry, West University of Timisoara, Oituz 4, 300086 Timisoara, Romania.
Ostafe V; Advanced Environmental Research Laboratories, Department of Biology-Chemistry, West University of Timisoara, Oituz 4, 300086 Timisoara, Romania.
Prodanović R; Faculty of Chemistry, University of Belgrade, Studentski trg 12-16, 11000 Belgrade, Serbia.
Fischer R; Institute for Biology VII, Molecular Biotechnology, RWTH Aachen University, Worringerweg 1, 52074 Aachen, Germany.; Departments of Biological Sciences and Chemistry, Purdue University, 207 S. Martin Jischke Dr., West Lafayette, IN 47907, USA.
Ostafe R; Institute for Biology VII, Molecular Biotechnology, RWTH Aachen University, Worringerweg 1, 52074 Aachen, Germany.; Purdue Institute of Inflammation, Immunology and Infectious Disease, Molecular Evolution, Protein Engineering and Production, Purdue University, 207 S. Martin Jischke Dr., West Lafayette, IN 47907, USA.
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Źródło :
International journal of molecular sciences [Int J Mol Sci] 2021 Mar 16; Vol. 22 (6). Date of Electronic Publication: 2021 Mar 16.
Typ publikacji :
Journal Article
MeSH Terms :
Directed Molecular Evolution*
Flow Cytometry*
High-Throughput Screening Assays*
Chitinases/*genetics
Catalytic Domain ; Cell Survival ; Cyclodextrins ; Fluorescent Dyes/metabolism ; Gene Library ; Models, Molecular ; Mutation/genetics ; Structural Homology, Protein ; Substrate Specificity ; Trisaccharides ; Umbelliferones
Czasopismo naukowe
Tytuł :
Computational design of enzymes for biotechnological applications.
Autorzy :
Planas-Iglesias J; Loschmidt Laboratories, Department of Experimental Biology and RECETOX, Faculty of Science, Masaryk University, Kamenice 5/A13, 625 00 Brno, Czech Republic; International Clinical Research Center, St. Anne's University Hospital Brno, Pekarska 53, 656 91 Brno, Czech Republic.
Marques SM; Loschmidt Laboratories, Department of Experimental Biology and RECETOX, Faculty of Science, Masaryk University, Kamenice 5/A13, 625 00 Brno, Czech Republic; International Clinical Research Center, St. Anne's University Hospital Brno, Pekarska 53, 656 91 Brno, Czech Republic.
Pinto GP; Loschmidt Laboratories, Department of Experimental Biology and RECETOX, Faculty of Science, Masaryk University, Kamenice 5/A13, 625 00 Brno, Czech Republic; International Clinical Research Center, St. Anne's University Hospital Brno, Pekarska 53, 656 91 Brno, Czech Republic.
Musil M; Loschmidt Laboratories, Department of Experimental Biology and RECETOX, Faculty of Science, Masaryk University, Kamenice 5/A13, 625 00 Brno, Czech Republic; International Clinical Research Center, St. Anne's University Hospital Brno, Pekarska 53, 656 91 Brno, Czech Republic; IT4Innovations Centre of Excellence, Faculty of Information Technology, Brno University of Technology, 61266 Brno, Czech Republic.
Stourac J; Loschmidt Laboratories, Department of Experimental Biology and RECETOX, Faculty of Science, Masaryk University, Kamenice 5/A13, 625 00 Brno, Czech Republic; International Clinical Research Center, St. Anne's University Hospital Brno, Pekarska 53, 656 91 Brno, Czech Republic.
Damborsky J; Loschmidt Laboratories, Department of Experimental Biology and RECETOX, Faculty of Science, Masaryk University, Kamenice 5/A13, 625 00 Brno, Czech Republic; International Clinical Research Center, St. Anne's University Hospital Brno, Pekarska 53, 656 91 Brno, Czech Republic. Electronic address: .
Bednar D; Loschmidt Laboratories, Department of Experimental Biology and RECETOX, Faculty of Science, Masaryk University, Kamenice 5/A13, 625 00 Brno, Czech Republic. Electronic address: .
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Źródło :
Biotechnology advances [Biotechnol Adv] 2021 Mar-Apr; Vol. 47, pp. 107696. Date of Electronic Publication: 2021 Jan 26.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't; Review
MeSH Terms :
Biotechnology*
Directed Molecular Evolution*
Biocatalysis ; Enzymes/genetics ; Enzymes/metabolism ; Mutagenesis ; Protein Engineering
Czasopismo naukowe
Tytuł :
Molecular biology interventions for activity improvement and production of industrial enzymes.
Autorzy :
Kant Bhatia S; Department of Biological Engineering, College of Engineering, Konkuk University, Seoul 05029, Republic of Korea; Institute for Ubiquitous Information Technology and Application, Konkuk University, Seoul 05029, Republic of Korea.
Vivek N; Centre for Climate and Environmental Protection, School of Water, Energy and Environment, Cranfield University, Cranfield MK43 0AL, UK.
Kumar V; Centre for Climate and Environmental Protection, School of Water, Energy and Environment, Cranfield University, Cranfield MK43 0AL, UK.
Chandel N; School of Medical and Allied Sciences, GD Goenka University, Gurugram 122103, Haryana, India.
Thakur M; Department of Biotechnology, Shoolini Institute of Life Sciences and Business Management, Solan 173212, Himachal Pradesh, India.
Kumar D; School of Bioengineering & Food Technology, Shoolini University of Biotechnology and Management Sciences, Solan 173229, Himachal Pradesh, India.
Yang YH; Department of Biological Engineering, College of Engineering, Konkuk University, Seoul 05029, Republic of Korea; Institute for Ubiquitous Information Technology and Application, Konkuk University, Seoul 05029, Republic of Korea.
Pugazendhi A; Innovative Green Product Synthesis and Renewable Environment Development Research Group, Faculty of Environment and Labour Safety, Ton Duc Thang University, Ho ChiMinh City, Viet Nam.
Kumar G; Institute of Chemistry, Bioscience and Environmental Engineering, Faculty of Science and Technology, University of Stavanger, Box 8600 Forus, 4036 Stavanger, Norway; School of Civil and Environmental Engineering, Yonsei University, Seoul 03722, Republic of Korea. Electronic address: .
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Źródło :
Bioresource technology [Bioresour Technol] 2021 Mar; Vol. 324, pp. 124596. Date of Electronic Publication: 2020 Dec 24.
Typ publikacji :
Journal Article; Review
MeSH Terms :
Directed Molecular Evolution*
Industry*
Catalysis ; Enzymes/genetics ; Enzymes/metabolism ; Molecular Biology ; Protein Engineering ; Substrate Specificity
Czasopismo naukowe
Tytuł :
[Recent advances of continuous in vivo evolution].
Autorzy :
Zhai H; State Key Laboratory of Microbial Technology, Shandong University, Qingdao 266273, Shandong, China.
Qi Q; State Key Laboratory of Microbial Technology, Shandong University, Qingdao 266273, Shandong, China.
Hou J; State Key Laboratory of Microbial Technology, Shandong University, Qingdao 266273, Shandong, China.
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Źródło :
Sheng wu gong cheng xue bao = Chinese journal of biotechnology [Sheng Wu Gong Cheng Xue Bao] 2021 Feb 25; Vol. 37 (2), pp. 486-499.
Typ publikacji :
Journal Article; Review
MeSH Terms :
Directed Molecular Evolution*
Proteins*
Humans ; Mutation ; Phenotype
Czasopismo naukowe
Tytuł :
Phage-assisted evolution of botulinum neurotoxin proteases with reprogrammed specificity.
Autorzy :
Blum TR; Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA.; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA.; Howard Hughes Medical Institute, Harvard University, Cambridge, MA 02138, USA.
Liu H; Department of Urology, Boston Children's Hospital, Boston, MA 02115, USA.; Department of Microbiology and Department of Surgery, Harvard Medical School, Boston, MA 02115, USA.
Packer MS; Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA.; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA.; Howard Hughes Medical Institute, Harvard University, Cambridge, MA 02138, USA.
Xiong X; Department of Urology, Boston Children's Hospital, Boston, MA 02115, USA.; Department of Microbiology and Department of Surgery, Harvard Medical School, Boston, MA 02115, USA.
Lee PG; Department of Urology, Boston Children's Hospital, Boston, MA 02115, USA.; Department of Microbiology and Department of Surgery, Harvard Medical School, Boston, MA 02115, USA.
Zhang S; Department of Urology, Boston Children's Hospital, Boston, MA 02115, USA.; Department of Microbiology and Department of Surgery, Harvard Medical School, Boston, MA 02115, USA.
Richter M; Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA.; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA.; Howard Hughes Medical Institute, Harvard University, Cambridge, MA 02138, USA.
Minasov G; Department of Microbiology-Immunology, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USA.
Satchell KJF; Department of Microbiology-Immunology, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USA.
Dong M; Department of Urology, Boston Children's Hospital, Boston, MA 02115, USA. .; Department of Microbiology and Department of Surgery, Harvard Medical School, Boston, MA 02115, USA.
Liu DR; Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA. .; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA.; Howard Hughes Medical Institute, Harvard University, Cambridge, MA 02138, USA.
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Źródło :
Science (New York, N.Y.) [Science] 2021 Feb 19; Vol. 371 (6531), pp. 803-810.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
MeSH Terms :
Directed Molecular Evolution*
Protein Engineering*
Botulinum Toxins/*metabolism
Animals ; Bacteriophage M13/genetics ; Botulinum Toxins/chemistry ; Botulinum Toxins/genetics ; Catalytic Domain ; Cell Line ; Cells, Cultured ; Humans ; Mutation ; Neurons/metabolism ; PTEN Phosphohydrolase/metabolism ; Peptide Library ; Protein Domains ; R-SNARE Proteins/metabolism ; Rats ; Selection, Genetic ; Substrate Specificity ; Vesicle-Associated Membrane Protein 2/metabolism
Czasopismo naukowe
Tytuł :
[Progress in the construction and screening of random mutation library].
Autorzy :
Chen J; Institute of Applied Genomics, Fuzhou University, Fuzhou 350108, Fujian, China.; College of Biological Science and Engineering, Fuzhou University, Fuzhou 350108, Fujian, China.
Huang J; Institute of Applied Genomics, Fuzhou University, Fuzhou 350108, Fujian, China.; College of Biological Science and Engineering, Fuzhou University, Fuzhou 350108, Fujian, China.
Yan T; Institute of Applied Genomics, Fuzhou University, Fuzhou 350108, Fujian, China.; College of Biological Science and Engineering, Fuzhou University, Fuzhou 350108, Fujian, China.
Peng X; Institute of Applied Genomics, Fuzhou University, Fuzhou 350108, Fujian, China.; College of Biological Science and Engineering, Fuzhou University, Fuzhou 350108, Fujian, China.
Lin J; Institute of Applied Genomics, Fuzhou University, Fuzhou 350108, Fujian, China.; College of Biological Science and Engineering, Fuzhou University, Fuzhou 350108, Fujian, China.; Fujian Key Laboratory of Marine Enzyme Engineering, Fuzhou University, Fuzhou 350108, Fujian, China.
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Źródło :
Sheng wu gong cheng xue bao = Chinese journal of biotechnology [Sheng Wu Gong Cheng Xue Bao] 2021 Jan 25; Vol. 37 (1), pp. 163-177.
Typ publikacji :
Journal Article; Review
MeSH Terms :
Directed Molecular Evolution*
Proteins*/genetics
Base Sequence ; Gene Library ; Mutation
Czasopismo naukowe
Tytuł :
Directed Evolution of a Selective and Sensitive Serotonin Sensor via Machine Learning.
Autorzy :
Unger EK; Departments of Biochemistry and Molecular Medicine, Chemistry, Statistics, Molecular and Cellular Biology, and Physiology and Membrane Biology, the Center for Neuroscience, and Graduate Programs in Molecular, Cellular, and Integrative Physiology, Biochemistry, Molecular, Cellular and Developmental Biology and Neuroscience, University of California, Davis, Davis, CA 95616, USA.
Keller JP; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20174, USA.
Altermatt M; Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
Liang R; Departments of Biochemistry and Molecular Medicine, Chemistry, Statistics, Molecular and Cellular Biology, and Physiology and Membrane Biology, the Center for Neuroscience, and Graduate Programs in Molecular, Cellular, and Integrative Physiology, Biochemistry, Molecular, Cellular and Developmental Biology and Neuroscience, University of California, Davis, Davis, CA 95616, USA.
Matsui A; Laboratory on Neurobiology of Compulsive Behaviors, National Institute on Alcohol Abuse and Alcoholism, NIH, Bethesda, MD 20892, USA.
Dong C; Departments of Biochemistry and Molecular Medicine, Chemistry, Statistics, Molecular and Cellular Biology, and Physiology and Membrane Biology, the Center for Neuroscience, and Graduate Programs in Molecular, Cellular, and Integrative Physiology, Biochemistry, Molecular, Cellular and Developmental Biology and Neuroscience, University of California, Davis, Davis, CA 95616, USA.
Hon OJ; Bowles Center for Alcohol Studies, Department of Pharmacology, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA.
Yao Z; Department of Chemistry, University of California, Irvine, Irvine, CA 92697, USA.
Sun J; Departments of Biochemistry and Molecular Medicine, Chemistry, Statistics, Molecular and Cellular Biology, and Physiology and Membrane Biology, the Center for Neuroscience, and Graduate Programs in Molecular, Cellular, and Integrative Physiology, Biochemistry, Molecular, Cellular and Developmental Biology and Neuroscience, University of California, Davis, Davis, CA 95616, USA.
Banala S; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20174, USA.
Flanigan ME; Bowles Center for Alcohol Studies, Department of Pharmacology, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA.
Jaffe DA; Departments of Biochemistry and Molecular Medicine, Chemistry, Statistics, Molecular and Cellular Biology, and Physiology and Membrane Biology, the Center for Neuroscience, and Graduate Programs in Molecular, Cellular, and Integrative Physiology, Biochemistry, Molecular, Cellular and Developmental Biology and Neuroscience, University of California, Davis, Davis, CA 95616, USA.
Hartanto S; Departments of Biochemistry and Molecular Medicine, Chemistry, Statistics, Molecular and Cellular Biology, and Physiology and Membrane Biology, the Center for Neuroscience, and Graduate Programs in Molecular, Cellular, and Integrative Physiology, Biochemistry, Molecular, Cellular and Developmental Biology and Neuroscience, University of California, Davis, Davis, CA 95616, USA.
Carlen J; Departments of Biochemistry and Molecular Medicine, Chemistry, Statistics, Molecular and Cellular Biology, and Physiology and Membrane Biology, the Center for Neuroscience, and Graduate Programs in Molecular, Cellular, and Integrative Physiology, Biochemistry, Molecular, Cellular and Developmental Biology and Neuroscience, University of California, Davis, Davis, CA 95616, USA.
Mizuno GO; Departments of Biochemistry and Molecular Medicine, Chemistry, Statistics, Molecular and Cellular Biology, and Physiology and Membrane Biology, the Center for Neuroscience, and Graduate Programs in Molecular, Cellular, and Integrative Physiology, Biochemistry, Molecular, Cellular and Developmental Biology and Neuroscience, University of California, Davis, Davis, CA 95616, USA.
Borden PM; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20174, USA.
Shivange AV; Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
Cameron LP; Departments of Biochemistry and Molecular Medicine, Chemistry, Statistics, Molecular and Cellular Biology, and Physiology and Membrane Biology, the Center for Neuroscience, and Graduate Programs in Molecular, Cellular, and Integrative Physiology, Biochemistry, Molecular, Cellular and Developmental Biology and Neuroscience, University of California, Davis, Davis, CA 95616, USA.
Sinning S; Department of Pharmacology, Yale University School of Medicine, New Haven, CT 06520, USA.
Underhill SM; Laboratory of Molecular and Cellular Neurobiology, National Institute on Mental Health, NIH, Bethesda, MD 20892, USA.
Olson DE; Departments of Biochemistry and Molecular Medicine, Chemistry, Statistics, Molecular and Cellular Biology, and Physiology and Membrane Biology, the Center for Neuroscience, and Graduate Programs in Molecular, Cellular, and Integrative Physiology, Biochemistry, Molecular, Cellular and Developmental Biology and Neuroscience, University of California, Davis, Davis, CA 95616, USA.
Amara SG; Laboratory of Molecular and Cellular Neurobiology, National Institute on Mental Health, NIH, Bethesda, MD 20892, USA.
Temple Lang D; Departments of Biochemistry and Molecular Medicine, Chemistry, Statistics, Molecular and Cellular Biology, and Physiology and Membrane Biology, the Center for Neuroscience, and Graduate Programs in Molecular, Cellular, and Integrative Physiology, Biochemistry, Molecular, Cellular and Developmental Biology and Neuroscience, University of California, Davis, Davis, CA 95616, USA.
Rudnick G; Department of Pharmacology, Yale University School of Medicine, New Haven, CT 06520, USA.
Marvin JS; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20174, USA.
Lavis LD; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20174, USA.
Lester HA; Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
Alvarez VA; Laboratory on Neurobiology of Compulsive Behaviors, National Institute on Alcohol Abuse and Alcoholism, NIH, Bethesda, MD 20892, USA.
Fisher AJ; Departments of Biochemistry and Molecular Medicine, Chemistry, Statistics, Molecular and Cellular Biology, and Physiology and Membrane Biology, the Center for Neuroscience, and Graduate Programs in Molecular, Cellular, and Integrative Physiology, Biochemistry, Molecular, Cellular and Developmental Biology and Neuroscience, University of California, Davis, Davis, CA 95616, USA.
Prescher JA; Department of Chemistry, University of California, Irvine, Irvine, CA 92697, USA.
Kash TL; Bowles Center for Alcohol Studies, Department of Pharmacology, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA.
Yarov-Yarovoy V; Departments of Biochemistry and Molecular Medicine, Chemistry, Statistics, Molecular and Cellular Biology, and Physiology and Membrane Biology, the Center for Neuroscience, and Graduate Programs in Molecular, Cellular, and Integrative Physiology, Biochemistry, Molecular, Cellular and Developmental Biology and Neuroscience, University of California, Davis, Davis, CA 95616, USA.
Gradinaru V; Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
Looger LL; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20174, USA. Electronic address: .
Tian L; Departments of Biochemistry and Molecular Medicine, Chemistry, Statistics, Molecular and Cellular Biology, and Physiology and Membrane Biology, the Center for Neuroscience, and Graduate Programs in Molecular, Cellular, and Integrative Physiology, Biochemistry, Molecular, Cellular and Developmental Biology and Neuroscience, University of California, Davis, Davis, CA 95616, USA. Electronic address: .
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Źródło :
Cell [Cell] 2020 Dec 23; Vol. 183 (7), pp. 1986-2002.e26. Date of Electronic Publication: 2020 Dec 16.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural; Research Support, N.I.H., Intramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
MeSH Terms :
Directed Molecular Evolution*
Machine Learning*
Serotonin/*metabolism
Algorithms ; Amino Acid Sequence ; Amygdala/physiology ; Animals ; Behavior, Animal ; Binding Sites ; Brain/metabolism ; HEK293 Cells ; Humans ; Kinetics ; Linear Models ; Mice ; Mice, Inbred C57BL ; Photons ; Protein Binding ; Serotonin Plasma Membrane Transport Proteins/metabolism ; Sleep/physiology ; Wakefulness/physiology
Czasopismo naukowe
Tytuł :
How directed evolution reshapes the energy landscape in an enzyme to boost catalysis.
Autorzy :
Otten R; Howard Hughes Medical Institute and Department of Biochemistry, Brandeis University, Waltham, MA 02454, USA.
Pádua RAP; Howard Hughes Medical Institute and Department of Biochemistry, Brandeis University, Waltham, MA 02454, USA.
Bunzel HA; Laboratory of Organic Chemistry, ETH Zürich, 8093 Zürich, Switzerland.
Nguyen V; Howard Hughes Medical Institute and Department of Biochemistry, Brandeis University, Waltham, MA 02454, USA.
Pitsawong W; Howard Hughes Medical Institute and Department of Biochemistry, Brandeis University, Waltham, MA 02454, USA.
Patterson M; Howard Hughes Medical Institute and Department of Biochemistry, Brandeis University, Waltham, MA 02454, USA.
Sui S; Department of Chemical Engineering, Institute of Applied Life Sciences, University of Massachusetts, Amherst, MA 01003, USA.
Perry SL; Department of Chemical Engineering, Institute of Applied Life Sciences, University of Massachusetts, Amherst, MA 01003, USA.
Cohen AE; Stanford Synchrotron Radiation Lightsource, Menlo Park, CA 94025, USA.
Hilvert D; Laboratory of Organic Chemistry, ETH Zürich, 8093 Zürich, Switzerland. .
Kern D; Howard Hughes Medical Institute and Department of Biochemistry, Brandeis University, Waltham, MA 02454, USA. .
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Źródło :
Science (New York, N.Y.) [Science] 2020 Dec 18; Vol. 370 (6523), pp. 1442-1446. Date of Electronic Publication: 2020 Nov 19.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
MeSH Terms :
Biocatalysis*
Computer-Aided Design*
Directed Molecular Evolution*
Enzymes/*chemistry
Enzymes/*genetics
Proteins/*chemistry
Proteins/*genetics
Catalytic Domain ; Nuclear Magnetic Resonance, Biomolecular ; Protein Conformation
Czasopismo naukowe
Tytuł :
Directed co-evolution of interacting protein-peptide pairs by compartmentalized two-hybrid replication (C2HR).
Autorzy :
Siau JW; p53 Laboratory, Agency for Science, Technology and Research (A*STAR), 8A Biomedical Grove, 138648, Singapore.
Nonis S; p53 Laboratory, Agency for Science, Technology and Research (A*STAR), 8A Biomedical Grove, 138648, Singapore.
Chee S; p53 Laboratory, Agency for Science, Technology and Research (A*STAR), 8A Biomedical Grove, 138648, Singapore.
Koh LQ; p53 Laboratory, Agency for Science, Technology and Research (A*STAR), 8A Biomedical Grove, 138648, Singapore.
Ferrer FJ; p53 Laboratory, Agency for Science, Technology and Research (A*STAR), 8A Biomedical Grove, 138648, Singapore.
Brown CJ; p53 Laboratory, Agency for Science, Technology and Research (A*STAR), 8A Biomedical Grove, 138648, Singapore.
Ghadessy FJ; p53 Laboratory, Agency for Science, Technology and Research (A*STAR), 8A Biomedical Grove, 138648, Singapore.
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Źródło :
Nucleic acids research [Nucleic Acids Res] 2020 Dec 16; Vol. 48 (22), pp. e128.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Directed Molecular Evolution*
Escherichia coli/*genetics
Peptides/*genetics
Protein Interaction Maps/*genetics
Cell Compartmentation/genetics ; DNA Replication/genetics ; Gene Expression Regulation, Bacterial/genetics ; Genotype ; Luciferases/genetics ; Phenotype ; Protein Engineering ; Taq Polymerase/genetics
Czasopismo naukowe
Tytuł :
UMI-linked consensus sequencing enables phylogenetic analysis of directed evolution.
Autorzy :
Zurek PJ; Department of Biochemistry, University of Cambridge, Cambridge, CB2 1GA, UK.; Johnson Matthey Plc, Cambridge, CB4 0WE, UK.
Knyphausen P; Department of Biochemistry, University of Cambridge, Cambridge, CB2 1GA, UK.
Neufeld K; Department of Biochemistry, University of Cambridge, Cambridge, CB2 1GA, UK.; Johnson Matthey Plc, Cambridge, CB4 0WE, UK.
Pushpanath A; Johnson Matthey Plc, Cambridge, CB4 0WE, UK.
Hollfelder F; Department of Biochemistry, University of Cambridge, Cambridge, CB2 1GA, UK. .
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Źródło :
Nature communications [Nat Commun] 2020 Nov 26; Vol. 11 (1), pp. 6023. Date of Electronic Publication: 2020 Nov 26.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Directed Molecular Evolution*
High-Throughput Nucleotide Sequencing/*methods
High-Throughput Screening Assays/*methods
Oxidoreductases Acting on CH-NH Group Donors/*genetics
Protein Engineering/*methods
Biocatalysis ; Cloning, Molecular ; Computational Biology/methods ; Consensus Sequence/genetics ; Datasets as Topic ; Enzyme Assays ; Epistasis, Genetic ; Gene Library ; Mutagenesis ; Mutation ; Oxidoreductases Acting on CH-NH Group Donors/isolation & purification ; Oxidoreductases Acting on CH-NH Group Donors/metabolism ; Phylogeny ; Recombinant Proteins/genetics ; Recombinant Proteins/isolation & purification ; Recombinant Proteins/metabolism ; Software
Czasopismo naukowe
Tytuł :
High Throughput Screening with SAMDI Mass Spectrometry for Directed Evolution.
Autorzy :
Pluchinsky AJ
Wackelin DJ; Division of Chemistry and Chemical Engineering MC 210-41, California Institute of Technology, Pasadena, California 91125, United States.
Huang X; Division of Chemistry and Chemical Engineering MC 210-41, California Institute of Technology, Pasadena, California 91125, United States.
Arnold FH; Division of Chemistry and Chemical Engineering MC 210-41, California Institute of Technology, Pasadena, California 91125, United States.
Mrksich M
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Źródło :
Journal of the American Chemical Society [J Am Chem Soc] 2020 Nov 25; Vol. 142 (47), pp. 19804-19808. Date of Electronic Publication: 2020 Nov 11.
Typ publikacji :
Journal Article; Research Support, U.S. Gov't, Non-P.H.S.
MeSH Terms :
Directed Molecular Evolution*
High-Throughput Screening Assays/*methods
Alkylation ; Carbon/chemistry ; Cytochrome P-450 Enzyme System/genetics ; Cytochrome P-450 Enzyme System/metabolism ; Escherichia coli/metabolism ; Hydrogen/chemistry ; Mutagenesis, Site-Directed ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Czasopismo naukowe
Tytuł :
Genetically Encoded Biosensor-Based Screening for Directed Bacteriophage T4 Lysozyme Evolution.
Autorzy :
Woo SG; Synthetic Biology and Bioengineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Korea.; Department of Biosystems and Bioengineering, KRIBB School of Biotechnology, University of Science and Technology (UST), Daejeon 34113, Korea.
Kim SK; Synthetic Biology and Bioengineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Korea.
Oh BR; Microbial Biotechnology Research Center, Jeonbuk Branch Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup 56212, Korea.
Lee SG; Synthetic Biology and Bioengineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Korea.; Department of Biosystems and Bioengineering, KRIBB School of Biotechnology, University of Science and Technology (UST), Daejeon 34113, Korea.
Lee DH; Synthetic Biology and Bioengineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Korea.; Department of Biosystems and Bioengineering, KRIBB School of Biotechnology, University of Science and Technology (UST), Daejeon 34113, Korea.
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Źródło :
International journal of molecular sciences [Int J Mol Sci] 2020 Nov 17; Vol. 21 (22). Date of Electronic Publication: 2020 Nov 17.
Typ publikacji :
Journal Article
MeSH Terms :
Bacteriophage T4*/enzymology
Bacteriophage T4*/growth & development
Biosensing Techniques*
Directed Molecular Evolution*
Escherichia coli*/enzymology
Escherichia coli*/genetics
Muramidase*/genetics
Muramidase*/metabolism
Viral Proteins*/genetics
Viral Proteins*/metabolism
Czasopismo naukowe
Tytuł :
Frequency-dependent selection can forecast evolution in Streptococcus pneumoniae.
Autorzy :
Azarian T; Burnett School of Biomedical Sciences, University of Central Florida, Orlando, Florida, United States of America.; Center for Communicable Disease Dynamics, Department of Epidemiology, T.H. Chan School of Public Health, Harvard University, Boston, Massachusetts, United States of America.
Martinez PP; Center for Communicable Disease Dynamics, Department of Epidemiology, T.H. Chan School of Public Health, Harvard University, Boston, Massachusetts, United States of America.
Arnold BJ; Center for Communicable Disease Dynamics, Department of Epidemiology, T.H. Chan School of Public Health, Harvard University, Boston, Massachusetts, United States of America.
Qiu X; Center for Communicable Disease Dynamics, Department of Epidemiology, T.H. Chan School of Public Health, Harvard University, Boston, Massachusetts, United States of America.
Grant LR; Center for American Indian Health, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, United States of America.
Corander J; Helsinki Institute for Information Technology, Department of Mathematics and Statistics, University of Helsinki, Helsinki, Finland.; Department of Biostatistics, University of Oslo, Oslo, Norway.; Infection Genomics, The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, United Kingdom.
Fraser C; Big Data Institute, Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom.
Croucher NJ; MRC Centre for Global Infectious Disease Analysis, Department of Infectious Disease Epidemiology, Imperial College London, London, United Kingdom.
Hammitt LL; Center for American Indian Health, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, United States of America.
Reid R; Center for American Indian Health, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, United States of America.
Santosham M; Center for American Indian Health, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, United States of America.
Weatherholtz RC; Center for American Indian Health, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, United States of America.
Bentley SD; Infection Genomics, The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, United Kingdom.
O'Brien KL; World Health Organization, Geneva, Switzerland.
Lipsitch M; Center for Communicable Disease Dynamics, Department of Epidemiology, T.H. Chan School of Public Health, Harvard University, Boston, Massachusetts, United States of America.; Department of Immunology and Infectious Diseases, T.H. Chan School of Public Health, Harvard University, Boston, Massachusetts, United States of America.
Hanage WP; Center for Communicable Disease Dynamics, Department of Epidemiology, T.H. Chan School of Public Health, Harvard University, Boston, Massachusetts, United States of America.
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Źródło :
PLoS biology [PLoS Biol] 2020 Oct 22; Vol. 18 (10), pp. e3000878. Date of Electronic Publication: 2020 Oct 22 (Print Publication: 2020).
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
MeSH Terms :
Directed Molecular Evolution*
Streptococcus pneumoniae/*physiology
Computer Simulation ; Models, Biological ; Pneumococcal Vaccines/immunology ; Streptococcus pneumoniae/immunology
Czasopismo naukowe
Tytuł :
A paper-based whole-cell screening assay for directed evolution-driven enzyme engineering.
Autorzy :
Gul I; School of Life Science and Technology, University of Electronic Science and Technology of China, No. 4, Section 2, North Jianshe Road, Chengdu, 610054, China.
Bogale TF; School of Life Science and Technology, University of Electronic Science and Technology of China, No. 4, Section 2, North Jianshe Road, Chengdu, 610054, China.
Chen Y; School of Life Science and Technology, University of Electronic Science and Technology of China, No. 4, Section 2, North Jianshe Road, Chengdu, 610054, China.
Yang X; School of Life Science and Technology, University of Electronic Science and Technology of China, No. 4, Section 2, North Jianshe Road, Chengdu, 610054, China.
Fang R; School of Life Science and Technology, University of Electronic Science and Technology of China, No. 4, Section 2, North Jianshe Road, Chengdu, 610054, China.; Center for Informational Biology, University of Electronic Science and Technology of China, Chengdu, 610054, China.
Feng J; School of Life Science and Technology, University of Electronic Science and Technology of China, No. 4, Section 2, North Jianshe Road, Chengdu, 610054, China.; Center for Informational Biology, University of Electronic Science and Technology of China, Chengdu, 610054, China.
Gao H; School of Computing Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054, China.; Center for Informational Biology, University of Electronic Science and Technology of China, Chengdu, 610054, China.
Tang L; School of Life Science and Technology, University of Electronic Science and Technology of China, No. 4, Section 2, North Jianshe Road, Chengdu, 610054, China. .; Center for Informational Biology, University of Electronic Science and Technology of China, Chengdu, 610054, China. .
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Źródło :
Applied microbiology and biotechnology [Appl Microbiol Biotechnol] 2020 Jul; Vol. 104 (13), pp. 6013-6022. Date of Electronic Publication: 2020 May 04.
Typ publikacji :
Journal Article
MeSH Terms :
Paper*
Directed Molecular Evolution/*methods
High-Throughput Screening Assays/*methods
Catalysis ; Colorimetry ; Directed Molecular Evolution/economics ; Directed Molecular Evolution/standards ; Escherichia coli/genetics ; Escherichia coli/metabolism ; Gene Library ; High-Throughput Screening Assays/economics ; High-Throughput Screening Assays/standards ; Hydrolases/genetics ; Hydrolases/metabolism ; Mutagenesis ; Mutation ; Reproducibility of Results ; Smartphone
Czasopismo naukowe
Tytuł :
Entropy of a bacterial stress response is a generalizable predictor for fitness and antibiotic sensitivity.
Autorzy :
Zhu Z; Biology Department, Boston College, Chestnut Hill, MA, 02467, USA.
Surujon D; Biology Department, Boston College, Chestnut Hill, MA, 02467, USA.
Ortiz-Marquez JC; Biology Department, Boston College, Chestnut Hill, MA, 02467, USA.
Huo W; Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, MA, 02111, USA.
Isberg RR; Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, MA, 02111, USA.
Bento J; Computer Science Department, Boston College, Chestnut Hill, MA, 02467, USA.
van Opijnen T; Biology Department, Boston College, Chestnut Hill, MA, 02467, USA. .
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Źródło :
Nature communications [Nat Commun] 2020 Aug 31; Vol. 11 (1), pp. 4365. Date of Electronic Publication: 2020 Aug 31.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural
MeSH Terms :
Directed Molecular Evolution*
Stress, Physiological*
Bacteria/*genetics
Drug Resistance, Bacterial/*genetics
Anti-Bacterial Agents/pharmacology ; Bacteria/drug effects ; Bacteria/metabolism ; Bacterial Physiological Phenomena ; Communicable Diseases/diagnosis ; Entropy ; Gene Expression Regulation, Bacterial ; Genes, Bacterial ; Genome, Bacterial ; Sequence Analysis, RNA ; Streptococcus pneumoniae/drug effects ; Streptococcus pneumoniae/genetics ; Streptococcus pneumoniae/metabolism ; Transcriptome
Czasopismo naukowe
Tytuł :
A colourimetric high-throughput screening system for directed evolution of prodigiosin ligase PigC.
Autorzy :
Brands S; Lehrstuhl für Biotechnologie, RWTH Aachen University, Worringerweg 3, 52074 Aachen, Germany. .
Brass HUC
Klein AS
Pietruszka J
Ruff AJ
Schwaneberg U
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Źródło :
Chemical communications (Cambridge, England) [Chem Commun (Camb)] 2020 Aug 07; Vol. 56 (61), pp. 8631-8634. Date of Electronic Publication: 2020 Jun 26.
Typ publikacji :
Journal Article
MeSH Terms :
Directed Molecular Evolution*
Bacterial Proteins/*metabolism
High-Throughput Screening Assays/*methods
Ligases/*metabolism
Bacterial Proteins/genetics ; Colorimetry ; Escherichia coli/metabolism ; Kinetics ; Ligases/genetics ; Mutagenesis, Site-Directed ; Prodigiosin/metabolism ; Pseudomonas putida/metabolism ; Substrate Specificity
Czasopismo naukowe
Tytuł :
A mechanistic view of enzyme evolution.
Autorzy :
Yang G; Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, Canada.
Miton CM; Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, Canada.
Tokuriki N; Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, Canada.
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Źródło :
Protein science : a publication of the Protein Society [Protein Sci] 2020 Aug; Vol. 29 (8), pp. 1724-1747.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't; Review
MeSH Terms :
Directed Molecular Evolution*
Enzymes*/chemistry
Enzymes*/genetics
Evolution, Molecular*
Protein Engineering*
Protein Domains ; Substrate Specificity
Czasopismo naukowe
Tytuł :
ARTP mutation and adaptive laboratory evolution improve probiotic performance of Bacillus coagulans.
Autorzy :
Liu K; School of Food and Biological Engineering, Jiangsu University, Zhenjiang, 212013, People's Republic of China.
Fang H; ShangHai YuanYao Biotechnology, Co., Ltd, Shanghai, 201316, People's Republic of China.
Cui F; School of Food and Biological Engineering, Jiangsu University, Zhenjiang, 212013, People's Republic of China. .
Nyabako BA; School of Food and Biological Engineering, Jiangsu University, Zhenjiang, 212013, People's Republic of China.
Tao T; School of Food and Biological Engineering, Jiangsu University, Zhenjiang, 212013, People's Republic of China.
Zan X; School of Food and Biological Engineering, Jiangsu University, Zhenjiang, 212013, People's Republic of China.
Chen H; Institute of Life Sciences, Jiangsu University, Zhenjiang, 212000, People's Republic of China.
Sun W; School of Food and Biological Engineering, Jiangsu University, Zhenjiang, 212013, People's Republic of China. .
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Źródło :
Applied microbiology and biotechnology [Appl Microbiol Biotechnol] 2020 Jul; Vol. 104 (14), pp. 6363-6373. Date of Electronic Publication: 2020 May 30.
Typ publikacji :
Journal Article
MeSH Terms :
Directed Molecular Evolution*
Probiotics*
Bacillus coagulans/*physiology
Plasma Gases/*pharmacology
Bacillus coagulans/drug effects ; Bacillus coagulans/genetics ; Bacillus coagulans/isolation & purification ; Bile Acids and Salts/metabolism ; Biofilms/growth & development ; Cell Membrane/chemistry ; Cell Membrane/metabolism ; Cell Membrane Permeability ; Fatty Acids/chemistry ; Hydrophobic and Hydrophilic Interactions ; Mutagenesis ; Mutation ; Temperature
Czasopismo naukowe

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