Temat: "Mutagenesis, Site-Directed" - OpacWWW

Skocz do pozycji: 61.

Tytuł:: Cys Site-Directed Mutagenesis of the Human SLC1A5 (ASCT2) Transporter: Structure/Function Relationships and Crucial Role of Cys467 for Redox Sensing and Glutamine Transport.
Autorzy:: Scalise M; Department DiBEST (Biologia, Ecologia, Scienze della Terra) Unit of Biochemistry and Molecular Biotechnology, University of Calabria, Via P. Bucci 4C, 87036 Arcavacata di Rende, Italy. .
Pochini L; Department DiBEST (Biologia, Ecologia, Scienze della Terra) Unit of Biochemistry and Molecular Biotechnology, University of Calabria, Via P. Bucci 4C, 87036 Arcavacata di Rende, Italy. .
Console L; Department DiBEST (Biologia, Ecologia, Scienze della Terra) Unit of Biochemistry and Molecular Biotechnology, University of Calabria, Via P. Bucci 4C, 87036 Arcavacata di Rende, Italy. .
Pappacoda G; Department DiBEST (Biologia, Ecologia, Scienze della Terra) Unit of Biochemistry and Molecular Biotechnology, University of Calabria, Via P. Bucci 4C, 87036 Arcavacata di Rende, Italy. .
Pingitore P; Department of Molecular and Clinical Medicine, Wallenberg Laboratory, Sahlgrenska Academy, University of Gothenburg, 413 45 Gothenburg, Sweden. .
Hedfalk K; Department of Chemistry and Molecular Biology, University of Gothenburg, P.O. Box 462, SE-405 30 Göteborg, Sweden. .
Indiveri C; Department DiBEST (Biologia, Ecologia, Scienze della Terra) Unit of Biochemistry and Molecular Biotechnology, University of Calabria, Via P. Bucci 4C, 87036 Arcavacata di Rende, Italy. .; CNR Institute of Biomembranes, Bioenergetics and Molecular Biotechnology, via Amendola 165/A, 70126 Bari, Italy. .; Pokaż więcej
Źródło:: International journal of molecular sciences [Int J Mol Sci] 2018 Feb 25; Vol. 19 (3). Date of Electronic Publication: 2018 Feb 25.
Typ publikacji:: Journal Article
MeSH Terms:: Mutagenesis, Site-Directed*
Amino Acid Transport System ASC/*chemistry
Amino Acid Transport System ASC/*genetics
Cysteine/*genetics
Minor Histocompatibility Antigens/*chemistry
Minor Histocompatibility Antigens/*genetics
Amino Acid Transport System ASC/metabolism ; Biological Transport/drug effects ; Disulfides/chemistry ; Energy Metabolism ; Glutamine/metabolism ; Glutamine/pharmacology ; Humans ; Kinetics ; Minor Histocompatibility Antigens/metabolism ; Models, Molecular ; Oxidation-Reduction ; Protein Conformation ; Structure-Activity Relationship ; Substrate Specificity

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Skocz do pozycji: 62.

Tytuł:: dbAMEPNI: a database of alanine mutagenic effects for protein-nucleic acid interactions.
Autorzy:: Liu L; School of Life Sciences, Anhui University, Hefei, Anhui 230601, China.
Xiong Y; State Key Laboratory of Microbial Metabolism and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
Gao H; Information and Engineering College, Dalian University, Dalian 116622, Liaoning, China.
Wei DQ; State Key Laboratory of Microbial Metabolism and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
Mitchell JC; Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, USA.; Department of Mathematics, University of Wisconsin-Madison, Madison, WI 53706, USA.; Oak Ridge National Laboratory, Biosciences Division, Oak Ridge, TN 37830, USA.
Zhu X; School of Life Sciences, Anhui University, Hefei, Anhui 230601, China.; Pokaż więcej
Źródło:: Database : the journal of biological databases and curation [Database (Oxford)] 2018 Jan 01; Vol. 2018.
Typ publikacji:: Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:: Alanine*
DNA-Binding Proteins*/chemistry
DNA-Binding Proteins*/genetics
DNA-Binding Proteins*/metabolism
Databases, Nucleic Acid*
Databases, Protein*
Mutagenesis, Site-Directed*
Mutation, Missense*
Amino Acid Substitution

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Skocz do pozycji: 63.

Tytuł:: Enhancement in catalytic activity of Aspergillus niger XynB by selective site-directed mutagenesis of active site amino acids.
Autorzy:: Wu X; The State Key Laboratory of Microbial Technology, Shandong University, 27 Shandanan Rd, Jinan, 250100, People's Republic of China.
Tian Z; The State Key Laboratory of Microbial Technology, Shandong University, 27 Shandanan Rd, Jinan, 250100, People's Republic of China.
Jiang X; The State Key Laboratory of Microbial Technology, Shandong University, 27 Shandanan Rd, Jinan, 250100, People's Republic of China.
Zhang Q; The State Key Laboratory of Microbial Technology, Shandong University, 27 Shandanan Rd, Jinan, 250100, People's Republic of China.
Wang L; The State Key Laboratory of Microbial Technology, Shandong University, 27 Shandanan Rd, Jinan, 250100, People's Republic of China. .; Pokaż więcej
Źródło:: Applied microbiology and biotechnology [Appl Microbiol Biotechnol] 2018 Jan; Vol. 102 (1), pp. 249-260. Date of Electronic Publication: 2017 Nov 04.
Typ publikacji:: Journal Article
MeSH Terms:: Mutagenesis, Site-Directed*
Amino Acids/*genetics
Aspergillus niger/*enzymology
Aspergillus niger/*genetics
Catalytic Domain/*genetics
Endo-1,4-beta Xylanases/*genetics
beta-Glucosidase/*genetics
Amino Acids/chemistry ; Amino Acids/metabolism ; Aspergillus niger/metabolism ; Catalysis ; Endo-1,4-beta Xylanases/metabolism ; Enzyme Stability ; Evolution, Molecular ; Kinetics ; Models, Molecular ; Mutation ; Protein Binding ; Protein Conformation ; beta-Glucosidase/metabolism

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Skocz do pozycji: 64.

Tytuł:: The role of cysteine residues in the allosteric modulation of the chromophore phototransformations of biphotochromic fluorescent protein SAASoti.
Autorzy:: Gavshina AV; A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow, Russia.
Marynich NK; A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow, Russia.
Khrenova MG; A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow, Russia.; Department of Chemistry, Lomonosov Moscow State University, Moscow, Russia.
Solovyev ID; A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow, Russia.
Savitsky AP; A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow, Russia. .; Pokaż więcej
Źródło:: Scientific reports [Sci Rep] 2021 Dec 21; Vol. 11 (1), pp. 24314. Date of Electronic Publication: 2021 Dec 21.
Typ publikacji:: Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:: Photochemical Processes*
Point Mutation*
Cysteine/*chemistry
Luminescent Proteins/*chemistry
Rhodophyta/*metabolism
Allosteric Site ; Cysteine/genetics ; Luminescent Proteins/genetics ; Luminescent Proteins/metabolism ; Mutagenesis, Site-Directed ; Protein Conformation

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Skocz do pozycji: 65.

Tytuł:: Combined effects of double mutations on catalytic activity and structural stability contribute to clinical manifestations of glucose-6-phosphate dehydrogenase deficiency.
Autorzy:: Pakparnich P; Department of Molecular Tropical Medicine and Genetics, Faculty of Tropical Medicine, Mahidol University, Bangkok, 10400, Thailand.
Sudsumrit S; Department of Molecular Tropical Medicine and Genetics, Faculty of Tropical Medicine, Mahidol University, Bangkok, 10400, Thailand.
Imwong M; Department of Molecular Tropical Medicine and Genetics, Faculty of Tropical Medicine, Mahidol University, Bangkok, 10400, Thailand.
Suteewong T; Department of Chemical Engineering, School of Engineering, King Mongkut's Institute of Technology Ladkrabang, Bangkok, 10520, Thailand.
Chamchoy K; Princess Srisavangavadhana College of Medicine, Chulabhorn Royal Academy, Bangkok, 10210, Thailand.
Pakotiprapha D; Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, 10400, Thailand.; Center for Excellence in Protein and Enzyme Technology, Faculty of Science, Mahidol University, Bangkok, 10400, Thailand.
Leartsakulpanich U; National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani, 12120, Thailand.
Boonyuen U; Department of Molecular Tropical Medicine and Genetics, Faculty of Tropical Medicine, Mahidol University, Bangkok, 10400, Thailand. .; Pokaż więcej
Źródło:: Scientific reports [Sci Rep] 2021 Dec 21; Vol. 11 (1), pp. 24307. Date of Electronic Publication: 2021 Dec 21.
Typ publikacji:: Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:: Mutation*
Glucosephosphate Dehydrogenase/*chemistry
Glucosephosphate Dehydrogenase/*genetics
Catalysis ; Glucosephosphate Dehydrogenase/metabolism ; Humans ; Mutagenesis, Site-Directed ; Oxidative Stress ; Protein Conformation

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Skocz do pozycji: 66.

Tytuł:: Structural and functional insights into the first Bacillus thuringiensis vegetative insecticidal protein of the Vpb4 fold, active against western corn rootworm.
Autorzy:: Kouadio JL; Bayer Crop Science, Chesterfield, Missouri, United States of America.
Zheng M; Bayer Crop Science, Chesterfield, Missouri, United States of America.
Aikins M; Department of Entomology, Kansas State University, Manhattan, Kansas, United States of America.
Duda D; Bayer Crop Science, Chesterfield, Missouri, United States of America.
Duff S; Bayer Crop Science, Chesterfield, Missouri, United States of America.
Chen D; Bayer Crop Science, Chesterfield, Missouri, United States of America.
Zhang J; Bayer Crop Science, Chesterfield, Missouri, United States of America.
Milligan J; Bayer Crop Science, Chesterfield, Missouri, United States of America.
Taylor C; Bayer Crop Science, Chesterfield, Missouri, United States of America.
Mamanella P; Bayer Crop Science, Chesterfield, Missouri, United States of America.
Rydel T; Bayer Crop Science, Chesterfield, Missouri, United States of America.
Kessenich C; Bayer Crop Science, Chesterfield, Missouri, United States of America.
Panosian T; Bayer Crop Science, Chesterfield, Missouri, United States of America.
Yin Y; Bayer Crop Science, Chesterfield, Missouri, United States of America.
Moar W; Bayer Crop Science, Chesterfield, Missouri, United States of America.
Giddings K; Bayer Crop Science, Chesterfield, Missouri, United States of America.
Park Y; Department of Entomology, Kansas State University, Manhattan, Kansas, United States of America.
Jerga A; Bayer Crop Science, Chesterfield, Missouri, United States of America.
Haas J; Bayer Crop Science, Chesterfield, Missouri, United States of America.; Pokaż więcej
Źródło:: PloS one [PLoS One] 2021 Dec 20; Vol. 16 (12), pp. e0260532. Date of Electronic Publication: 2021 Dec 20 (Print Publication: 2021).
Typ publikacji:: Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:: Bacillus thuringiensis/*metabolism
Bacterial Proteins/*chemistry
Insecticides/*pharmacology
Amino Acid Sequence ; Animals ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Coleoptera/drug effects ; Coleoptera/growth & development ; Crystallography, X-Ray ; Insecticides/chemistry ; Intestines/metabolism ; Larva/drug effects ; Larva/metabolism ; Mutagenesis, Site-Directed ; Protein Multimerization ; Protein Structure, Tertiary ; Recombinant Proteins/biosynthesis ; Recombinant Proteins/isolation & purification ; Recombinant Proteins/pharmacology ; Zea mays/metabolism ; Zea mays/parasitology

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Skocz do pozycji: 67.

Tytuł:: A Systematic Approach to Assess the Activity and Classification of PCSK9 Variants.
Autorzy:: Uribe KB; Department of Molecular Biophysics, Biofisika Institute, University of Basque Country and Consejo Superior de Investigaciones Científicas (UPV/EHU, CSIC), 48940 Leioa, Spain.; Center for Cooperative Research in Biomaterials (CIC biomaGUNE), Basque Research and Technology Alliance (BRTA), 20014 Donostia San Sebastian, Spain.
Chemello K; Inserm, UMR 1188 Diabète Athérothrombose Thérapies Réunion Océan Indien (DéTROI), Université de La Réunion, 97400 Saint-Denis de La Reunion, France.
Larrea-Sebal A; Department of Molecular Biophysics, Biofisika Institute, University of Basque Country and Consejo Superior de Investigaciones Científicas (UPV/EHU, CSIC), 48940 Leioa, Spain.; Fundación Biofisika Bizkaia, 48940 Leioa, Spain.
Benito-Vicente A; Department of Molecular Biophysics, Biofisika Institute, University of Basque Country and Consejo Superior de Investigaciones Científicas (UPV/EHU, CSIC), 48940 Leioa, Spain.; Department of Biochemistry and Molecular Biology, Universidad del País Vasco UPV/EHU, 48080 Bilbao, Spain.
Galicia-Garcia U; Department of Molecular Biophysics, Biofisika Institute, University of Basque Country and Consejo Superior de Investigaciones Científicas (UPV/EHU, CSIC), 48940 Leioa, Spain.; Fundación Biofisika Bizkaia, 48940 Leioa, Spain.
Bourane S; Inserm, UMR 1188 Diabète Athérothrombose Thérapies Réunion Océan Indien (DéTROI), Université de La Réunion, 97400 Saint-Denis de La Reunion, France.
Jaafar AK; Inserm, UMR 1188 Diabète Athérothrombose Thérapies Réunion Océan Indien (DéTROI), Université de La Réunion, 97400 Saint-Denis de La Reunion, France.
Lambert G; Inserm, UMR 1188 Diabète Athérothrombose Thérapies Réunion Océan Indien (DéTROI), Université de La Réunion, 97400 Saint-Denis de La Reunion, France.
Martín C; Department of Molecular Biophysics, Biofisika Institute, University of Basque Country and Consejo Superior de Investigaciones Científicas (UPV/EHU, CSIC), 48940 Leioa, Spain.; Department of Biochemistry and Molecular Biology, Universidad del País Vasco UPV/EHU, 48080 Bilbao, Spain.; Pokaż więcej
Źródło:: International journal of molecular sciences [Int J Mol Sci] 2021 Dec 18; Vol. 22 (24). Date of Electronic Publication: 2021 Dec 18.
Typ publikacji:: Journal Article
MeSH Terms:: Proprotein Convertase 9/*genetics
Gain of Function Mutation ; HEK293 Cells ; Hep G2 Cells ; Humans ; Hyperlipoproteinemia Type II/genetics ; Hyperlipoproteinemia Type II/metabolism ; Mutagenesis, Site-Directed ; Mutation ; Proprotein Convertase 9/metabolism ; Receptors, LDL/metabolism

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Skocz do pozycji: 68.

Tytuł:: Polar Interactions at the Dimer-Dimer Interface of Methionine Adenosyltransferase MAT I Control Tetramerization.
Autorzy:: Sánchez-Pérez GF; Instituto de Investigaciones Biomédicas Alberto Sols (CSIC-UAM), Arturo Duperier 4, 28029 Madrid, Spain.; Hudson River Biotechnology, Nieuwe Kanaal 7V, 6709 PA Wageningen, The Netherlands.
Pajares MÁ; Instituto de Investigaciones Biomédicas Alberto Sols (CSIC-UAM), Arturo Duperier 4, 28029 Madrid, Spain.; Centro de Investigaciones Biológicas Margarita Salas (CSIC), Ramiro de Maeztu 9, 28040 Madrid, Spain.; Pokaż więcej
Źródło:: International journal of molecular sciences [Int J Mol Sci] 2021 Dec 08; Vol. 22 (24). Date of Electronic Publication: 2021 Dec 08.
Typ publikacji:: Journal Article
MeSH Terms:: Amino Acid Substitution*
Methionine Adenosyltransferase/*chemistry
Methionine Adenosyltransferase/*metabolism
Animals ; Methionine Adenosyltransferase/genetics ; Models, Molecular ; Mutagenesis, Site-Directed ; Protein Multimerization ; Protein Structure, Secondary ; Protein Structure, Tertiary ; Rats

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Skocz do pozycji: 69.

Tytuł:: Experimental Characterization of the Interaction between the N-Terminal SH3 Domain of Crkl and C3G.
Autorzy:: Pagano L; Istituto Pasteur-Fondazione Cenci Bolognetti, Dipartimento di Scienze Biochimiche 'A. Rossi Fanelli' and Istituto di Biologia e Patologia Molecolari del CNR, Sapienza Università di Roma, 00185 Rome, Italy.
Malagrinò F; Istituto Pasteur-Fondazione Cenci Bolognetti, Dipartimento di Scienze Biochimiche 'A. Rossi Fanelli' and Istituto di Biologia e Patologia Molecolari del CNR, Sapienza Università di Roma, 00185 Rome, Italy.
Nardella C; Istituto Pasteur-Fondazione Cenci Bolognetti, Dipartimento di Scienze Biochimiche 'A. Rossi Fanelli' and Istituto di Biologia e Patologia Molecolari del CNR, Sapienza Università di Roma, 00185 Rome, Italy.
Gianni S; Istituto Pasteur-Fondazione Cenci Bolognetti, Dipartimento di Scienze Biochimiche 'A. Rossi Fanelli' and Istituto di Biologia e Patologia Molecolari del CNR, Sapienza Università di Roma, 00185 Rome, Italy.
Toto A; Istituto Pasteur-Fondazione Cenci Bolognetti, Dipartimento di Scienze Biochimiche 'A. Rossi Fanelli' and Istituto di Biologia e Patologia Molecolari del CNR, Sapienza Università di Roma, 00185 Rome, Italy.; Pokaż więcej
Źródło:: International journal of molecular sciences [Int J Mol Sci] 2021 Dec 07; Vol. 22 (24). Date of Electronic Publication: 2021 Dec 07.
Typ publikacji:: Journal Article
MeSH Terms:: Adaptor Proteins, Signal Transducing/*chemistry
Adaptor Proteins, Signal Transducing/*metabolism
Guanine Nucleotide-Releasing Factor 2/*metabolism
Mutagenesis, Site-Directed/*methods
Adaptor Proteins, Signal Transducing/genetics ; Binding Sites ; Cell Adhesion ; Cell Differentiation ; Cell Proliferation ; Guanine Nucleotide-Releasing Factor 2/chemistry ; Humans ; Models, Molecular ; Protein Binding ; Protein Conformation ; Protein Domains ; Static Electricity

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Skocz do pozycji: 70.

Tytuł:: Chimeric Investigations into the Diamide Binding Site on the Lepidopteran Ryanodine Receptor.
Autorzy:: Richardson E; Department of Biointeractions and Crop Protection, Rothamsted Research, Harpenden AL5 2JQ, UK.
Troczka BJ; Department of Biointeractions and Crop Protection, Rothamsted Research, Harpenden AL5 2JQ, UK.; Department of Biosciences, College of Life and Environmental Sciences, Penryn Campus, University of Exeter, Penryn TR10 9FW, UK.
Gutbrod O; Bayer AG, Crop Science Division, R&D, D-40789 Monheim, Germany.
Ebbinghaus-Kintscher U; Bayer AG, Crop Science Division, R&D, D-40789 Monheim, Germany.
Williamson MS; Department of Biointeractions and Crop Protection, Rothamsted Research, Harpenden AL5 2JQ, UK.
George CH; Institute of Life Sciences, Swansea University Medical School, Swansea SA2 8PP, UK.
Nauen R; Bayer AG, Crop Science Division, R&D, D-40789 Monheim, Germany.
Davies TGE; Department of Biointeractions and Crop Protection, Rothamsted Research, Harpenden AL5 2JQ, UK.; Pokaż więcej
Źródło:: International journal of molecular sciences [Int J Mol Sci] 2021 Dec 02; Vol. 22 (23). Date of Electronic Publication: 2021 Dec 02.
Typ publikacji:: Journal Article
MeSH Terms:: Diamide/*chemistry
Insect Proteins/*chemistry
Ryanodine Receptor Calcium Release Channel/*chemistry
Animals ; Binding Sites ; Caffeine/pharmacology ; Calcium Signaling/drug effects ; Diamide/metabolism ; Diamide/pharmacology ; Humans ; Insect Proteins/genetics ; Insect Proteins/metabolism ; Insecticide Resistance/drug effects ; Insecticides/chemistry ; Insecticides/metabolism ; Insecticides/pharmacology ; Moths/metabolism ; Mutagenesis, Site-Directed ; Recombinant Fusion Proteins/biosynthesis ; Recombinant Fusion Proteins/chemistry ; Recombinant Fusion Proteins/genetics ; Ryanodine Receptor Calcium Release Channel/genetics ; Ryanodine Receptor Calcium Release Channel/metabolism ; ortho-Aminobenzoates/chemistry ; ortho-Aminobenzoates/metabolism ; ortho-Aminobenzoates/pharmacology

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Skocz do pozycji: 71.

Tytuł:: Structural and biochemical analyses of the tetrameric carboxypeptidase S9Cfn from Fusobacterium nucleatum.
Autorzy:: Wang X; Department of Stomatology, The Second Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230601, People's Republic of China.
Cheng MT; Hefei National Laboratory for Physical Sciences at the Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230027, People's Republic of China.
Chen ZP; Hefei National Laboratory for Physical Sciences at the Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230027, People's Republic of China.
Jiang YL; Hefei National Laboratory for Physical Sciences at the Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230027, People's Republic of China.
Ge YS; Hefei National Laboratory for Physical Sciences at the Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230027, People's Republic of China.
Xia R; Department of Stomatology, The Second Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230601, People's Republic of China.
Hou WT; Hefei National Laboratory for Physical Sciences at the Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230027, People's Republic of China.; Pokaż więcej
Źródło:: Acta crystallographica. Section D, Structural biology [Acta Crystallogr D Struct Biol] 2021 Dec 01; Vol. 77 (Pt 12), pp. 1554-1563. Date of Electronic Publication: 2021 Nov 17.
Typ publikacji:: Journal Article
MeSH Terms:: Bacterial Proteins/*metabolism
Carboxypeptidases/*metabolism
Fusobacterium nucleatum/*enzymology
Amino Acid Sequence ; Bacterial Proteins/chemistry ; Carboxypeptidases/chemistry ; Carboxypeptidases/genetics ; Catalytic Domain ; Crystallography, X-Ray ; Humans ; Mass Spectrometry ; Mutagenesis, Site-Directed ; Protein Conformation

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Skocz do pozycji: 72.

Tytuł:: Flavonoids and Acid-Hydrolysis derivatives of Neo -Clerodane diterpenes from Teucrium flavum subsp. glaucum as inhibitors of the HIV-1 reverse transcriptase-associated RNase H function.
Autorzy:: Fois B; Department of Life and Environmental Sciences, University of Cagliari, Cittadella Universitaria di Monserrato, Monserrato, Italy.
Corona A; Department of Life and Environmental Sciences, University of Cagliari, Cittadella Universitaria di Monserrato, Monserrato, Italy.
Tramontano E; Department of Life and Environmental Sciences, University of Cagliari, Cittadella Universitaria di Monserrato, Monserrato, Italy.; Istituto di Ricerca Genetica e Biomedica, Consiglio Nazionale delle Ricerche, Monserrato, Italy.
Distinto S; Department of Life and Environmental Sciences, University of Cagliari, Cittadella Universitaria di Monserrato, Monserrato, Italy.
Maccioni E; Department of Life and Environmental Sciences, University of Cagliari, Cittadella Universitaria di Monserrato, Monserrato, Italy.
Meleddu R; Department of Life and Environmental Sciences, University of Cagliari, Cittadella Universitaria di Monserrato, Monserrato, Italy.
Caboni P; Department of Life and Environmental Sciences, University of Cagliari, Cittadella Universitaria di Monserrato, Monserrato, Italy.
Floris C; Department of Chemical and Geological Sciences, University of Cagliari, Monserrato, Italy.
Cottiglia F; Department of Life and Environmental Sciences, University of Cagliari, Cittadella Universitaria di Monserrato, Monserrato, Italy.; Pokaż więcej
Źródło:: Journal of enzyme inhibition and medicinal chemistry [J Enzyme Inhib Med Chem] 2021 Dec; Vol. 36 (1), pp. 749-757.
Typ publikacji:: Journal Article
MeSH Terms:: Diterpenes, Clerodane/*pharmacology
Flavonoids/*pharmacology
HIV Reverse Transcriptase/*antagonists & inhibitors
Plant Extracts/*pharmacology
Reverse Transcriptase Inhibitors/*pharmacology
Ribonuclease H/*antagonists & inhibitors
Teucrium/*chemistry
Diterpenes, Clerodane/chemistry ; Diterpenes, Clerodane/isolation & purification ; Dose-Response Relationship, Drug ; Flavonoids/chemistry ; Flavonoids/isolation & purification ; HIV Reverse Transcriptase/genetics ; HIV Reverse Transcriptase/metabolism ; Hydrogen-Ion Concentration ; Hydrolysis ; Models, Molecular ; Molecular Conformation ; Mutagenesis, Site-Directed ; Plant Extracts/chemistry ; Plant Extracts/isolation & purification ; Reverse Transcriptase Inhibitors/chemistry ; Reverse Transcriptase Inhibitors/isolation & purification ; Ribonuclease H/genetics ; Ribonuclease H/metabolism ; Structure-Activity Relationship

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Skocz do pozycji: 73.

Tytuł:: Biallelic inheritance in a single Pakistani family with intellectual disability implicates new candidate gene RDH14.
Autorzy:: Pastore SF; Molecular Neuropsychiatry and Development (MiND) Lab, Campbell Family Mental Health Research Institute, Centre for Addiction and Mental Health, 250 College Street, Toronto, ON, M5T 1R8, Canada.; Institute of Medical Science, University of Toronto, Toronto, ON, Canada.
Muhammad T; Molecular Neuropsychiatry and Development (MiND) Lab, Campbell Family Mental Health Research Institute, Centre for Addiction and Mental Health, 250 College Street, Toronto, ON, M5T 1R8, Canada.; Institute of Medical Science, University of Toronto, Toronto, ON, Canada.
Harripaul R; Molecular Neuropsychiatry and Development (MiND) Lab, Campbell Family Mental Health Research Institute, Centre for Addiction and Mental Health, 250 College Street, Toronto, ON, M5T 1R8, Canada.; Institute of Medical Science, University of Toronto, Toronto, ON, Canada.
Lau R; Molecular Neuropsychiatry and Development (MiND) Lab, Campbell Family Mental Health Research Institute, Centre for Addiction and Mental Health, 250 College Street, Toronto, ON, M5T 1R8, Canada.; Institute of Medical Science, University of Toronto, Toronto, ON, Canada.
Khan MTM; Department of Pathology, North-West School of Medicine, Hayatabad, Peshawar, Khyber Pakhtunkhwa, Pakistan.
Khan MI; Department of Zoology, Islamia College Peshawar, Peshawar, Khyber Pakhtunkhwa, Pakistan.
Islam O; Department of Diagnostic Radiology, Queen's University, Kingston, ON, Canada.
Kang C; Department of Biology and Institute of Basic Sciences, Sungshin Women's University, Seoul, Republic of Korea.
Ayub M; Department of Psychiatry, Queen's University Kingston, Kingston, ON, Canada.
Jelani M; Centre for Omic Sciences, Islamia College Peshawar, Peshawar, Khyber Pakhtunkhwa, Pakistan.
Vincent JB; Molecular Neuropsychiatry and Development (MiND) Lab, Campbell Family Mental Health Research Institute, Centre for Addiction and Mental Health, 250 College Street, Toronto, ON, M5T 1R8, Canada. .; Institute of Medical Science, University of Toronto, Toronto, ON, Canada. .; Department of Psychiatry, University of Toronto, Toronto, ON, Canada. .; Pokaż więcej
Źródło:: Scientific reports [Sci Rep] 2021 Nov 30; Vol. 11 (1), pp. 23113. Date of Electronic Publication: 2021 Nov 30.
Typ publikacji:: Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:: Alcohol Oxidoreductases*/genetics
Intellectual Disability*/genetics
Receptors, G-Protein-Coupled*/genetics
Adolescent ; Child ; Child, Preschool ; Female ; Humans ; Infant ; Male ; Alleles ; Brain/diagnostic imaging ; Brain/metabolism ; Cell Nucleus/metabolism ; Cerebellum/pathology ; Chlorides ; Chromosome Mapping ; Cytoplasm/metabolism ; Frameshift Mutation ; Genetic Variation ; Genotype ; HEK293 Cells ; Homozygote ; Ions ; Magnetic Resonance Imaging ; Mutagenesis, Site-Directed ; Mutation ; Oligonucleotide Array Sequence Analysis ; Pakistan ; Pedigree ; Retina/metabolism ; RNA, Small Interfering/metabolism ; Signal Transduction ; Tretinoin/metabolism ; Exome Sequencing

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Skocz do pozycji: 74.

Tytuł:: Analysis of Hendra Virus Fusion Protein N-Terminal Transmembrane Residues.
Autorzy:: Barrett CT; Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, KY 40502, USA.
Neal HE; Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, KY 40502, USA.
Edmonds K; Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, KY 40502, USA.
Zamora JLR; Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, KY 40502, USA.
Moncman CL; Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, KY 40502, USA.
Popa A; Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, KY 40502, USA.
Smith EC; Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, KY 40502, USA.
Webb SR; Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, KY 40502, USA.
Dutch RE; Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, KY 40502, USA.; Pokaż więcej
Źródło:: Viruses [Viruses] 2021 Nov 24; Vol. 13 (12). Date of Electronic Publication: 2021 Nov 24.
Typ publikacji:: Journal Article; Research Support, N.I.H., Extramural
MeSH Terms:: Membrane Fusion*
Hendra Virus/*genetics
Henipavirus Infections/*virology
Viral Fusion Proteins/*metabolism
Alanine/genetics ; Amino Acid Sequence ; Amino Acid Substitution ; Animals ; Cell Membrane/metabolism ; Chlorocebus aethiops ; Endocytosis ; Endosomes/metabolism ; Genes, Reporter ; Hendra Virus/physiology ; Humans ; Mutagenesis, Site-Directed ; Protein Domains ; Protein Stability ; Vero Cells ; Viral Fusion Proteins/genetics

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Skocz do pozycji: 75.

Tytuł:: The Methionine 549 and Leucine 552 Residues of Friedelin Synthase from Maytenus ilicifolia Are Important for Substrate Binding Specificity.
Autorzy:: Mazzeu BF; Instituto de Química, Universidade Estadual Paulista-UNESP, CP 355, Araraquara 14800-900, SP, Brazil.
Souza-Moreira TM; Instituto de Química, Universidade Estadual Paulista-UNESP, CP 355, Araraquara 14800-900, SP, Brazil.
Oliveira AA; Centro de Pesquisa e Inovação em Biodiversidade e Fármacos, Instituto de Física de São Carlos, Universidade de São Paulo, São Carlos 13563-120, SP, Brazil.
Remlinger M; Instituto de Química, Universidade Estadual Paulista-UNESP, CP 355, Araraquara 14800-900, SP, Brazil.
Felippe LG; Instituto de Química, Universidade Estadual Paulista-UNESP, CP 355, Araraquara 14800-900, SP, Brazil.
Valentini SR; Faculdade de Ciências Farmacêuticas, Universidade Estadual Paulista-UNESP, Rod. Araraquara-Jaú km 1, Araraquara 14800-903, SP, Brazil.
Guido RVC; Centro de Pesquisa e Inovação em Biodiversidade e Fármacos, Instituto de Física de São Carlos, Universidade de São Paulo, São Carlos 13563-120, SP, Brazil.
Zanelli CF; Faculdade de Ciências Farmacêuticas, Universidade Estadual Paulista-UNESP, Rod. Araraquara-Jaú km 1, Araraquara 14800-903, SP, Brazil.
Furlan M; Instituto de Química, Universidade Estadual Paulista-UNESP, CP 355, Araraquara 14800-900, SP, Brazil.; Pokaż więcej
Źródło:: Molecules (Basel, Switzerland) [Molecules] 2021 Nov 11; Vol. 26 (22). Date of Electronic Publication: 2021 Nov 11.
Typ publikacji:: Journal Article
MeSH Terms:: Alkyl and Aryl Transferases/*metabolism
Maytenus/*enzymology
Plant Proteins/*metabolism
Triterpenes/*metabolism
Alkyl and Aryl Transferases/chemistry ; Alkyl and Aryl Transferases/genetics ; Amino Acid Substitution ; Biosynthetic Pathways ; Cyclization ; Genes, Plant ; Leucine/chemistry ; Maytenus/genetics ; Methionine/chemistry ; Models, Molecular ; Mutagenesis, Site-Directed ; Oleanolic Acid/analogs & derivatives ; Oleanolic Acid/biosynthesis ; Pentacyclic Triterpenes/metabolism ; Plant Proteins/chemistry ; Plant Proteins/genetics ; Protein Structure, Secondary ; Recombinant Proteins/chemistry ; Recombinant Proteins/genetics ; Recombinant Proteins/metabolism ; Substrate Specificity

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Skocz do pozycji: 76.

Tytuł:: Glutamate Dehydrogenase Functions in Glutamic Acid Metabolism and Stress Resistance in Pyropia haitanensis .
Autorzy:: Li S; CAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China.; University of Chinese Academy of Sciences, Beijing 100049, China.
Shao Z; CAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China.
Lu C; CAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China.; University of Chinese Academy of Sciences, Beijing 100049, China.
Yao J; CAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China.
Zhou Y; Yancheng Hairui Food Co., Ltd., Dafeng 224005, China.
Duan D; CAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China.; Pokaż więcej
Źródło:: Molecules (Basel, Switzerland) [Molecules] 2021 Nov 10; Vol. 26 (22). Date of Electronic Publication: 2021 Nov 10.
Typ publikacji:: Journal Article
MeSH Terms:: Glutamate Dehydrogenase/*metabolism
Glutamic Acid/*metabolism
Rhodophyta/*metabolism
Biochemical Phenomena ; China ; Glutamate Dehydrogenase/physiology ; Mutagenesis, Site-Directed ; Rhodophyta/genetics ; Stress, Physiological/physiology

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Skocz do pozycji: 77.

Tytuł:: Mutations in the binding site of TNFR1 PLAD reduce homologous interactions but can enhance antagonism of wild-type TNFR1 activity.
Autorzy:: Albogami S; School of Life Sciences, University of Nottingham, Nottingham, UK.; Department of Biotechnology, School of Science, Taif University, Taif, Saudi Arabia.
Todd I; School of Life Sciences, University of Nottingham, Nottingham, UK.
Negm O; School of Life Sciences, University of Nottingham, Nottingham, UK.; Microbiology and Immunology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
Fairclough LC; School of Life Sciences, University of Nottingham, Nottingham, UK.
Tighe PJ; School of Life Sciences, University of Nottingham, Nottingham, UK.; Pokaż więcej
Źródło:: Immunology [Immunology] 2021 Nov; Vol. 164 (3), pp. 637-654. Date of Electronic Publication: 2021 Aug 25.
Typ publikacji:: Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:: Protein Domains/*genetics
Receptors, Tumor Necrosis Factor, Type I/*metabolism
Signal Transduction/*immunology
Binding Sites/genetics ; Cell Line, Tumor ; Humans ; Mutagenesis, Site-Directed ; Protein Multimerization/genetics ; Protein Multimerization/immunology ; Receptors, Tumor Necrosis Factor, Type I/genetics ; Tumor Necrosis Factor-alpha/metabolism

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Skocz do pozycji: 78.

Tytuł:: Monoclonal Antibody Targeting the HA191/199 Region of H1N1 Influenza Virus Mediates the Damage of Neural Cells.
Autorzy:: Guo CY; Central Laboratory of Shaanxi Provincial People's Hospital, Xi'an, Shaanxi, 710068, China. .; Shaanxi Province Research Center of Cell Immunological Engineering and Technology, Xi'an, Shaanxi, 710068, China.
Feng Q; Central Laboratory of Shaanxi Provincial People's Hospital, Xi'an, Shaanxi, 710068, China.; Shaanxi Province Research Center of Cell Immunological Engineering and Technology, Xi'an, Shaanxi, 710068, China.
Yan LT; Central Laboratory of Shaanxi Provincial People's Hospital, Xi'an, Shaanxi, 710068, China.; Shaanxi Province Research Center of Cell Immunological Engineering and Technology, Xi'an, Shaanxi, 710068, China.
Xie X; Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Xi'an, Shaanxi, 710069, China.
Liang DY; Central Laboratory of Shaanxi Provincial People's Hospital, Xi'an, Shaanxi, 710068, China.; Shaanxi Province Research Center of Cell Immunological Engineering and Technology, Xi'an, Shaanxi, 710068, China.
Li Y; Central Laboratory of Shaanxi Provincial People's Hospital, Xi'an, Shaanxi, 710068, China.; Shaanxi Province Research Center of Cell Immunological Engineering and Technology, Xi'an, Shaanxi, 710068, China.
Feng YM; Central Laboratory of Shaanxi Provincial People's Hospital, Xi'an, Shaanxi, 710068, China.; Shaanxi Province Research Center of Cell Immunological Engineering and Technology, Xi'an, Shaanxi, 710068, China.
Sun LJ; Central Laboratory of Shaanxi Provincial People's Hospital, Xi'an, Shaanxi, 710068, China. .; Shaanxi Province Research Center of Cell Immunological Engineering and Technology, Xi'an, Shaanxi, 710068, China.
Hu J; Central Laboratory of Shaanxi Provincial People's Hospital, Xi'an, Shaanxi, 710068, China. .; Shaanxi Province Research Center of Cell Immunological Engineering and Technology, Xi'an, Shaanxi, 710068, China.; Pokaż więcej
Źródło:: Biochemistry. Biokhimiia [Biochemistry (Mosc)] 2021 Nov; Vol. 86 (11), pp. 1469-1476.
Typ publikacji:: Journal Article
MeSH Terms:: Antibodies, Monoclonal, Murine-Derived*/chemistry
Antibodies, Monoclonal, Murine-Derived*/immunology
Hemagglutinin Glycoproteins, Influenza Virus*/chemistry
Hemagglutinin Glycoproteins, Influenza Virus*/genetics
Hemagglutinin Glycoproteins, Influenza Virus*/immunology
Influenza A Virus, H1N1 Subtype*/chemistry
Influenza A Virus, H1N1 Subtype*/genetics
Influenza A Virus, H1N1 Subtype*/immunology
Molecular Dynamics Simulation*
Neurons/*metabolism
Antibodies, Viral/chemistry ; Antibodies, Viral/immunology ; Cell Line, Tumor ; Humans ; Mutagenesis, Site-Directed ; Neurons/pathology ; Protein Domains

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Skocz do pozycji: 79.

Tytuł:: Site-saturation mutagenesis of mutant L-asparaginase II signal peptide hydrophobic region for improved excretion of cyclodextrin glucanotransferase.
Autorzy:: Ismail A; Department of Bioprocess and Polymer Engineering, Faculty of Chemical and Energy Engineering, Universiti Teknologi Malaysia, 81310, Skudai, Johor, Malaysia.
Illias RM; Department of Bioprocess and Polymer Engineering, Faculty of Chemical and Energy Engineering, Universiti Teknologi Malaysia, 81310, Skudai, Johor, Malaysia. .; Pokaż więcej
Źródło:: Journal of industrial microbiology & biotechnology [J Ind Microbiol Biotechnol] 2017 Dec; Vol. 44 (12), pp. 1627-1641. Date of Electronic Publication: 2017 Sep 18.
Typ publikacji:: Journal Article
MeSH Terms:: Hydrophobic and Hydrophilic Interactions*
Mutagenesis, Site-Directed*
Mutation*
Asparaginase/*genetics
Glucosyltransferases/*genetics
Glucosyltransferases/*metabolism
Protein Sorting Signals/*genetics
Asparaginase/chemistry ; Asparaginase/metabolism ; Escherichia coli/genetics ; Escherichia coli/metabolism ; Glucosyltransferases/chemistry ; Protein Sorting Signals/physiology ; Recombinant Proteins/biosynthesis ; Recombinant Proteins/genetics ; Recombinant Proteins/metabolism

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Skocz do pozycji: 80.

Tytuł:: Site-Directed Mutagenesis of the Fibronectin Domains in Insulin Receptor-Related Receptor.
Autorzy:: Deyev IE; Group of Molecular Physiology, Institute of Bioorganic Chemistry, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences, 117997 Moscow, Russia. .
Chachina NA; Laboratory of Receptor Cell Biology, Institute of Bioorganic Chemistry, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences, 117997 Moscow, Russia. n_.
Zhevlenev ES; Group of Molecular Physiology, Institute of Bioorganic Chemistry, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences, 117997 Moscow, Russia. .
Petrenko AG; Laboratory of Receptor Cell Biology, Institute of Bioorganic Chemistry, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences, 117997 Moscow, Russia. .; Pokaż więcej
Źródło:: International journal of molecular sciences [Int J Mol Sci] 2017 Nov 19; Vol. 18 (11). Date of Electronic Publication: 2017 Nov 19.
Typ publikacji:: Journal Article
MeSH Terms:: Mutagenesis, Site-Directed*
Fibronectins/*chemistry
Receptor, Insulin/*chemistry
Receptor, Insulin/*genetics
Amino Acid Sequence ; Glycosylation ; HEK293 Cells ; Humans ; Hydrogen-Ion Concentration ; Mutant Proteins/chemistry ; Protein Domains ; Structure-Activity Relationship

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