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Tytuł :
Processing Hundreds of SARS-CoV-2 Samples with an In-House PCR-Based Method without Robotics.
Autorzy :
Mair T; Institute of Biophysics, Johannes Kepler University, 4020 Linz, Austria.
Ivankovic M; Institute of Biophysics, Johannes Kepler University, 4020 Linz, Austria.
Paar C; Institute of Laboratory Medicine, Johannes Kepler Universitätsklinikum, 4020 Linz, Austria.
Salzer HJF; Department of Pulmonology, Johannes Kepler Universitätsklinikum, 4020 Linz, Austria.
Heissl A; Department of Biosciences, University of Salzburg, 5020 Salzburg, Austria.
Lamprecht B; Department of Pulmonology, Johannes Kepler Universitätsklinikum, 4020 Linz, Austria.
Schreier-Lechner E; Institute of Laboratory Medicine, Johannes Kepler Universitätsklinikum, 4020 Linz, Austria.
Tiemann-Boege I; Institute of Biophysics, Johannes Kepler University, 4020 Linz, Austria.
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Źródło :
Viruses [Viruses] 2021 Aug 28; Vol. 13 (9). Date of Electronic Publication: 2021 Aug 28.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Polymerase Chain Reaction*/methods
Polymerase Chain Reaction*/standards
SARS-CoV-2*/genetics
COVID-19/*diagnosis
COVID-19/*virology
COVID-19 Nucleic Acid Testing/*methods
COVID-19 Nucleic Acid Testing/standards ; Humans ; Reagent Kits, Diagnostic ; Real-Time Polymerase Chain Reaction/methods ; Real-Time Polymerase Chain Reaction/standards ; Reproducibility of Results ; Sensitivity and Specificity ; Viral Load
Czasopismo naukowe
Tytuł :
Specific allelic discrimination of N501Y and other SARS-CoV-2 mutations by ddPCR detects B.1.1.7 lineage in Washington State.
Autorzy :
Perchetti GA; Department of Laboratory Medicine and Pathology, Virology Division, University of Washington School of Medicine, Seattle, Washington, USA.
Zhu H; Department of Laboratory Medicine and Pathology, Virology Division, University of Washington School of Medicine, Seattle, Washington, USA.
Mills MG; Department of Laboratory Medicine and Pathology, Virology Division, University of Washington School of Medicine, Seattle, Washington, USA.
Shrestha L; Department of Laboratory Medicine and Pathology, Virology Division, University of Washington School of Medicine, Seattle, Washington, USA.
Wagner C; Department of Genome Sciences, University of Washington, Seattle, Washington, USA.; Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.
Bakhash SM; Department of Laboratory Medicine and Pathology, Virology Division, University of Washington School of Medicine, Seattle, Washington, USA.
Lin MJ; Department of Laboratory Medicine and Pathology, Virology Division, University of Washington School of Medicine, Seattle, Washington, USA.
Xie H; Department of Laboratory Medicine and Pathology, Virology Division, University of Washington School of Medicine, Seattle, Washington, USA.
Huang ML; Department of Laboratory Medicine and Pathology, Virology Division, University of Washington School of Medicine, Seattle, Washington, USA.
Mathias P; Department of Laboratory Medicine and Pathology, Virology Division, University of Washington School of Medicine, Seattle, Washington, USA.; Department of Biomedical Informatics and Medical Education, University of Washington School of Medicine, Seattle, Washington, USA.
Bedford T; Department of Genome Sciences, University of Washington, Seattle, Washington, USA.; Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.
Jerome KR; Department of Laboratory Medicine and Pathology, Virology Division, University of Washington School of Medicine, Seattle, Washington, USA.; Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.
Greninger AL; Department of Laboratory Medicine and Pathology, Virology Division, University of Washington School of Medicine, Seattle, Washington, USA.; Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.
Roychoudhury P; Department of Laboratory Medicine and Pathology, Virology Division, University of Washington School of Medicine, Seattle, Washington, USA.; Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.
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Źródło :
Journal of medical virology [J Med Virol] 2021 Oct; Vol. 93 (10), pp. 5931-5941. Date of Electronic Publication: 2021 Jul 03.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
COVID-19 Nucleic Acid Testing*
Real-Time Polymerase Chain Reaction*
SARS-CoV-2/*isolation & purification
Alleles ; COVID-19/diagnosis ; COVID-19/epidemiology ; Genotype ; High-Throughput Nucleotide Sequencing ; Humans ; Mutation ; Phylogeny ; Reverse Transcriptase Polymerase Chain Reaction ; SARS-CoV-2/genetics ; Spike Glycoprotein, Coronavirus/genetics ; Time Factors ; Washington/epidemiology
SCR Organism :
SARS-CoV-2 variants
Czasopismo naukowe
Tytuł :
Development of a dual-labeled, hydrolysis probe-based, real-time quantitative PCR assay for detection of both genotypes of duck circovirus-1 (DuCV-1) and DuCV-2.
Autorzy :
Zhang L; Shandong Agricultural University, Tai'an, Shandong, China.
Jiang W; China Animal Health and Epidemiology Center, Qingdao, Shandong, China.
Zhang F; Shandong Agricultural University, Tai'an, Shandong, China.
Li Y; China Animal Health and Epidemiology Center, Qingdao, Shandong, China.
Li J; China Animal Health and Epidemiology Center, Qingdao, Shandong, China.
Liang S; Inner Mongolia Agricultural University, Huhhot, Inner Mongolia, China.
Yu X; China Animal Health and Epidemiology Center, Qingdao, Shandong, China.
Peng C; China Animal Health and Epidemiology Center, Qingdao, Shandong, China.
Liu S; China Animal Health and Epidemiology Center, Qingdao, Shandong, China.
Wang J; China Animal Health and Epidemiology Center, Qingdao, Shandong, China.
Sun S; Shandong Agricultural University, Tai'an, Shandong, China. .
Liu H; China Animal Health and Epidemiology Center, Qingdao, Shandong, China. .
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Źródło :
Virus genes [Virus Genes] 2021 Oct; Vol. 57 (5), pp. 453-458. Date of Electronic Publication: 2021 Jul 26.
Typ publikacji :
Journal Article
MeSH Terms :
Polymerase Chain Reaction*
Circoviridae Infections/*diagnosis
Circovirus/*isolation & purification
Poultry Diseases/*diagnosis
Animals ; Circoviridae Infections/genetics ; Circoviridae Infections/virology ; Circovirus/genetics ; Circovirus/pathogenicity ; DNA, Viral/genetics ; Genotype ; Hydrolysis ; Poultry Diseases/genetics ; Poultry Diseases/virology ; Real-Time Polymerase Chain Reaction
SCR Organism :
Duck circovirus
Czasopismo naukowe
Tytuł :
DNA barcode, multiplex PCR and qPCR assay for diagnosis of pathogens infecting pulse crops to facilitate safe exchange and healthy conservation of germplasm.
Autorzy :
Tripathi A; Division of Plant Quarantine, ICAR-National Bureau of Plant Genetic Resources, New Delhi, 110 012, India.
Rai A; Division of Plant Quarantine, ICAR-National Bureau of Plant Genetic Resources, New Delhi, 110 012, India.
Dubey SC; Division of Plant Quarantine, ICAR-National Bureau of Plant Genetic Resources, New Delhi, 110 012, India. .
Akhtar J; Division of Plant Quarantine, ICAR-National Bureau of Plant Genetic Resources, New Delhi, 110 012, India.
Kumar P; Division of Plant Quarantine, ICAR-National Bureau of Plant Genetic Resources, New Delhi, 110 012, India.
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Źródło :
Archives of microbiology [Arch Microbiol] 2021 Jul; Vol. 203 (5), pp. 2575-2589. Date of Electronic Publication: 2021 Mar 08.
Typ publikacji :
Journal Article
MeSH Terms :
DNA Barcoding, Taxonomic*
Polymerase Chain Reaction*
Crops, Agricultural/*microbiology
Fabaceae/*microbiology
Fungi/*classification
Plant Diseases/*microbiology
Alternaria/classification ; Alternaria/genetics ; Alternaria/isolation & purification ; Ascomycota/classification ; Ascomycota/genetics ; Ascomycota/isolation & purification ; DNA, Fungal/genetics ; Fungi/genetics ; Fungi/isolation & purification ; Fusarium/classification ; Fusarium/genetics ; Fusarium/isolation & purification ; Multiplex Polymerase Chain Reaction ; Phylogeny ; Real-Time Polymerase Chain Reaction ; Rhizoctonia/classification ; Rhizoctonia/genetics ; Rhizoctonia/isolation & purification
SCR Organism :
Alternaria alternata; Ascochyta rabiei; Fusarium oxysporum; Macrophomina phaseolina; Pseudocercospora nelumbonicola; Rhizoctonia solani; Sclerotinia sclerotiorum
Czasopismo naukowe
Tytuł :
SARS-CoV-2 detection in wastewater using multiplex quantitative PCR.
Autorzy :
Navarro A; European Commission Joint Research Centre, Via E. Fermi 2749, 21027 Ispra, VA, Italy.
Gómez L; European Commission Joint Research Centre, Via E. Fermi 2749, 21027 Ispra, VA, Italy.
Sanseverino I; European Commission Joint Research Centre, Via E. Fermi 2749, 21027 Ispra, VA, Italy.
Niegowska M; European Commission Joint Research Centre, Via E. Fermi 2749, 21027 Ispra, VA, Italy.
Roka E; Department of Public Health Laboratory, National Public Health Centre, Albert Flórián út 2-6, 1097 Budapest, Hungary.
Pedraccini R; Alfa S.r.l., via Carrobbio, 3, 21100 Varese, Italy.
Vargha M; Department of Public Health Laboratory, National Public Health Centre, Albert Flórián út 2-6, 1097 Budapest, Hungary.
Lettieri T; European Commission Joint Research Centre, Via E. Fermi 2749, 21027 Ispra, VA, Italy. Electronic address: .
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Źródło :
The Science of the total environment [Sci Total Environ] 2021 Nov 25; Vol. 797, pp. 148890. Date of Electronic Publication: 2021 Jul 14.
Typ publikacji :
Journal Article
MeSH Terms :
COVID-19*
Multiplex Polymerase Chain Reaction*
Humans ; Real-Time Polymerase Chain Reaction ; SARS-CoV-2 ; Sensitivity and Specificity ; Waste Water
Czasopismo naukowe
Tytuł :
Implementation of a Sample Pooling Strategy for the Direct Detection of SARS-CoV-2 by Real-Time Polymerase Chain Reaction During the COVID-19 Pandemic.
Autorzy :
Chan CW; Department of Pathology, Chicago, IL, USA.
Kwon S; Department of Pathology, Chicago, IL, USA.
Matushek SM; Clinical Microbiology and Immunology Laboratory, University of Chicago, Chicago, IL, USA.
Ciaglia C; Clinical Microbiology and Immunology Laboratory, University of Chicago, Chicago, IL, USA.
Bethel C; Clinical Microbiology and Immunology Laboratory, University of Chicago, Chicago, IL, USA.
Beavis KG; Department of Pathology, Chicago, IL, USA.
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Źródło :
American journal of clinical pathology [Am J Clin Pathol] 2021 Jun 17; Vol. 156 (1), pp. 15-23.
Typ publikacji :
Journal Article
MeSH Terms :
COVID-19 Testing*
Real-Time Polymerase Chain Reaction*/methods
COVID-19/*diagnosis
SARS-CoV-2/*genetics
COVID-19/genetics ; Clinical Laboratory Techniques/methods ; Humans ; RNA, Viral/genetics ; Reverse Transcriptase Polymerase Chain Reaction/methods ; SARS-CoV-2/pathogenicity ; Specimen Handling/methods
Czasopismo naukowe
Tytuł :
Rapid and sensitive identification of pleural and peritoneal infections by droplet digital PCR.
Autorzy :
Zhou F; College of Medical Technology, Zhejiang Chinese Medical University, Hangzhou, 310053, Zhejiang, People's Republic of China.
Sun S; Department of Clinical Laboratory, The First Affiliated Hospital of Zhejiang Chinese Medical University, 54 Youdian Road, Hangzhou, 310006, Zhejiang, People's Republic of China.; The First Clinical Medical College of Zhejiang Chinese Medical University, Hangzhou, 310053, Zhejiang, People's Republic of China.
Sun X; Department of Clinical Laboratory, The First Affiliated Hospital of Zhejiang Chinese Medical University, 54 Youdian Road, Hangzhou, 310006, Zhejiang, People's Republic of China.
Chen Y; Department of Clinical Laboratory, The First Affiliated Hospital of Zhejiang Chinese Medical University, 54 Youdian Road, Hangzhou, 310006, Zhejiang, People's Republic of China.; The First Clinical Medical College of Zhejiang Chinese Medical University, Hangzhou, 310053, Zhejiang, People's Republic of China.
Yang X; Department of Clinical Laboratory, The First Affiliated Hospital of Zhejiang Chinese Medical University, 54 Youdian Road, Hangzhou, 310006, Zhejiang, People's Republic of China. .
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Źródło :
Folia microbiologica [Folia Microbiol (Praha)] 2021 Apr; Vol. 66 (2), pp. 213-219. Date of Electronic Publication: 2020 Nov 07.
Typ publikacji :
Journal Article
MeSH Terms :
Real-Time Polymerase Chain Reaction*
Humans ; Polymerase Chain Reaction ; Sensitivity and Specificity
Czasopismo naukowe
Tytuł :
Prevalence of respiratory viruses by Multiplex PCR: a four-and-a-half year retrospective study in an Italian general hospital.
Autorzy :
Leli C; Microbiology Laboratory, Azienda Ospedaliera SS. Antonio e Biagio e Cesare Arrigo, Alessandria, Italy.
Di Matteo L; Microbiology Laboratory, Azienda Ospedaliera SS. Antonio e Biagio e Cesare Arrigo, Alessandria, Italy.
Gotta F; Microbiology Laboratory, Azienda Ospedaliera SS. Antonio e Biagio e Cesare Arrigo, Alessandria, Italy.
Vay D; Microbiology Laboratory, Azienda Ospedaliera SS. Antonio e Biagio e Cesare Arrigo, Alessandria, Italy.
Piceghello A; Microbiology Laboratory, Azienda Ospedaliera SS. Antonio e Biagio e Cesare Arrigo, Alessandria, Italy.
Cornaglia E; Microbiology Laboratory, Azienda Ospedaliera SS. Antonio e Biagio e Cesare Arrigo, Alessandria, Italy.
Cavallo V; Microbiology Laboratory, Azienda Ospedaliera SS. Antonio e Biagio e Cesare Arrigo, Alessandria, Italy.
Busso S; Microbiology Laboratory, Azienda Ospedaliera SS. Antonio e Biagio e Cesare Arrigo, Alessandria, Italy.
Carrabba L; Microbiology Laboratory, Azienda Ospedaliera SS. Antonio e Biagio e Cesare Arrigo, Alessandria, Italy.
Mazzeo R; Microbiology Laboratory, Azienda Ospedaliera SS. Antonio e Biagio e Cesare Arrigo, Alessandria, Italy.
Rocchetti A; Microbiology Laboratory, Azienda Ospedaliera SS. Antonio e Biagio e Cesare Arrigo, Alessandria, Italy.
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Źródło :
Le infezioni in medicina [Infez Med] 2021 Mar 01; Vol. 29 (1), pp. 94-101.
Typ publikacji :
Journal Article
MeSH Terms :
Multiplex Polymerase Chain Reaction*
Respiratory Tract Infections*/diagnosis
Virus Diseases*/diagnosis
Adult ; Aged ; Child ; Coinfection ; Female ; Hospitals, General ; Humans ; Italy ; Male ; Prevalence ; Real-Time Polymerase Chain Reaction ; Retrospective Studies
Czasopismo naukowe
Tytuł :
End-point RT-PCR: A potential alternative for diagnosing coronavirus disease 2019 (COVID-19).
Autorzy :
Silva Júnior JVJ; Setor de Virologia, Laboratório de Imunopatologia Keizo Asami, Universidade Federal de Pernambuco, Pernambuco, Brazil; Departamento de Microbiologia e Parasitologia, Universidade Federal de Santa Maria, Rio Grande do Sul, Brazil; Setor de Virologia, Departamento de Medicina Veterinária Preventiva, Universidade Federal de Santa Maria, Rio Grande do Sul, Brazil.
Merchioratto I; Setor de Virologia, Departamento de Medicina Veterinária Preventiva, Universidade Federal de Santa Maria, Rio Grande do Sul, Brazil.
de Oliveira PSB; Setor de Virologia, Departamento de Medicina Veterinária Preventiva, Universidade Federal de Santa Maria, Rio Grande do Sul, Brazil.
Rocha Lopes TR; Setor de Virologia, Laboratório de Imunopatologia Keizo Asami, Universidade Federal de Pernambuco, Pernambuco, Brazil; Setor de Virologia, Departamento de Medicina Veterinária Preventiva, Universidade Federal de Santa Maria, Rio Grande do Sul, Brazil.
Brites PC; Laboratório de Análises Clínicas, Setor de Biologia Molecular, Hospital Universitário de Santa Maria, Rio Grande do Sul, Brazil.
de Oliveira EM; Laboratório de Análises Clínicas, Setor de Biologia Molecular, Hospital Universitário de Santa Maria, Rio Grande do Sul, Brazil.
Weiblen R; Setor de Virologia, Departamento de Medicina Veterinária Preventiva, Universidade Federal de Santa Maria, Rio Grande do Sul, Brazil.
Flores EF; Setor de Virologia, Departamento de Medicina Veterinária Preventiva, Universidade Federal de Santa Maria, Rio Grande do Sul, Brazil. Electronic address: .
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Źródło :
Journal of virological methods [J Virol Methods] 2021 Feb; Vol. 288, pp. 114007. Date of Electronic Publication: 2020 Oct 29.
Typ publikacji :
Journal Article
MeSH Terms :
Reverse Transcriptase Polymerase Chain Reaction*
COVID-19/*diagnosis
COVID-19/*virology
COVID-19 Nucleic Acid Testing/*methods
SARS-CoV-2/*genetics
Humans ; RNA, Viral ; Real-Time Polymerase Chain Reaction ; Sensitivity and Specificity
Czasopismo naukowe
Tytuł :
Point-of-care tests detecting HIV nucleic acids for diagnosis of HIV-1 or HIV-2 infection in infants and children aged 18 months or less.
Autorzy :
Ochodo EA; Centre for Global Health Research, Kenya Medical Research Institute, Kisumu, Kenya.; Centre for Evidence-based Health Care, Department of Global Health, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, South Africa.
Guleid F; KEMRI-Wellcome Trust Research Programme, Nairobi, Kenya.
Deeks JJ; Test Evaluation Research Group, Institute of Applied Health Research, University of Birmingham, Birmingham, UK.
Mallett S; UCL Centre for Medical Imaging, Division of Medicine, Faculty of Medical Sciences, University College London, London, UK.
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Źródło :
The Cochrane database of systematic reviews [Cochrane Database Syst Rev] 2021 Aug 12; Vol. 8. Cochrane AN: CD013207. Date of Electronic Publication: 2021 Aug 12.
Typ publikacji :
Journal Article; Meta-Analysis; Research Support, Non-U.S. Gov't; Review
MeSH Terms :
Point-of-Care Testing*
HIV Infections/*diagnosis
HIV-1/*genetics
HIV-2/*genetics
Polymerase Chain Reaction/*methods
Cross-Sectional Studies ; Female ; HIV-1/isolation & purification ; HIV-2/isolation & purification ; Humans ; Infant ; Infant, Newborn ; Male ; Reverse Transcriptase Polymerase Chain Reaction ; Sensitivity and Specificity
Czasopismo naukowe
Tytuł :
Novel dual multiplex real-time RT-PCR assays for the rapid detection of SARS-CoV-2, influenza A/B, and respiratory syncytial virus using the BD MAX open system.
Autorzy :
Chung HY; Division of Clinical Pathology, Department of Pathology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, ROC.
Jian MJ; Division of Clinical Pathology, Department of Pathology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, ROC.
Chang CK; Division of Clinical Pathology, Department of Pathology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, ROC.
Lin JC; Division of Infectious Diseases and Tropical Medicine, Department of Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, ROC.
Yeh KM; Division of Infectious Diseases and Tropical Medicine, Department of Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, ROC.
Chen CW; Division of Pulmonary and Critical Care Medicine, Department of Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, ROC.
Chiu SK; Division of Infectious Diseases and Tropical Medicine, Department of Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, ROC.
Wang YH; Division of Clinical Pathology, Department of Pathology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, ROC.
Liao SJ; Division of Clinical Pathology, Department of Pathology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, ROC.
Li SY; Division of Clinical Pathology, Department of Pathology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, ROC.
Hsieh SS; Division of Clinical Pathology, Department of Pathology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, ROC.
Tsai SH; Department of Emergency Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, ROC.
Perng CL; Division of Clinical Pathology, Department of Pathology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, ROC.
Yang JR; Centers for Disease Control, Taipei, Taiwan, ROC.
Liu MT; Centers for Disease Control, Taipei, Taiwan, ROC.
Chang FY; Division of Infectious Diseases and Tropical Medicine, Department of Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, ROC.
Shang HS; Division of Clinical Pathology, Department of Pathology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, ROC.
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Źródło :
Emerging microbes & infections [Emerg Microbes Infect] 2021 Dec; Vol. 10 (1), pp. 161-166.
Typ publikacji :
Journal Article
MeSH Terms :
COVID-19/*diagnosis
Influenza, Human/*diagnosis
Polymerase Chain Reaction/*methods
Respiratory Syncytial Virus Infections/*diagnosis
SARS-CoV-2/*genetics
Adolescent ; Adult ; Aged ; Aged, 80 and over ; COVID-19 Testing ; Coinfection/epidemiology ; Female ; Humans ; Male ; Middle Aged ; Multiplex Polymerase Chain Reaction/methods ; Real-Time Polymerase Chain Reaction/methods ; Reverse Transcriptase Polymerase Chain Reaction/methods ; Young Adult
Czasopismo naukowe
Tytuł :
Comparison of PCR-based methods for the detection of Babesia caballi and Theileria equi in field samples collected in Central Italy.
Autorzy :
Nardini R; Istituto Zooprofilattico Sperimentale del Lazio e della Toscana 'M. Aleandri', Via Appia Nuova 1411, 00178, Rome, Italy. .
Bartolomé Del Pino LE; Complutense University of Madrid, Madrid, Spain.
Cersini A; Istituto Zooprofilattico Sperimentale del Lazio e della Toscana 'M. Aleandri', Via Appia Nuova 1411, 00178, Rome, Italy.
Manna G; Istituto Zooprofilattico Sperimentale del Lazio e della Toscana 'M. Aleandri', Via Appia Nuova 1411, 00178, Rome, Italy.
Viola MR; Istituto Zooprofilattico Sperimentale del Lazio e della Toscana 'M. Aleandri', Via Appia Nuova 1411, 00178, Rome, Italy.
Antognetti V; Istituto Zooprofilattico Sperimentale del Lazio e della Toscana 'M. Aleandri', Via Appia Nuova 1411, 00178, Rome, Italy.
Autorino GL; Istituto Zooprofilattico Sperimentale del Lazio e della Toscana 'M. Aleandri', Via Appia Nuova 1411, 00178, Rome, Italy.
Scicluna MT; Istituto Zooprofilattico Sperimentale del Lazio e della Toscana 'M. Aleandri', Via Appia Nuova 1411, 00178, Rome, Italy.
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Źródło :
Parasitology research [Parasitol Res] 2021 Jun; Vol. 120 (6), pp. 2157-2164. Date of Electronic Publication: 2021 Apr 15.
Typ publikacji :
Comparative Study; Journal Article; Validation Study
MeSH Terms :
Babesia/*isolation & purification
Babesiosis/*parasitology
Horse Diseases/*parasitology
Polymerase Chain Reaction/*veterinary
Theileria/*isolation & purification
Theileriasis/*parasitology
Animals ; Babesia/genetics ; Babesiosis/epidemiology ; Horse Diseases/epidemiology ; Horses ; Italy/epidemiology ; Molecular Diagnostic Techniques/veterinary ; Polymerase Chain Reaction/methods ; Real-Time Polymerase Chain Reaction/veterinary ; Retrospective Studies ; Theileria/genetics ; Theileriasis/epidemiology
Czasopismo naukowe
Tytuł :
Correlating qRT-PCR, dPCR and Viral Titration for the Identification and Quantification of SARS-CoV-2: A New Approach for Infection Management.
Autorzy :
Brandolini M; Unit of Microbiology, The Great Romagna Hub Laboratory, 47522 Pievesestina, Italy.
Taddei F; Unit of Microbiology, The Great Romagna Hub Laboratory, 47522 Pievesestina, Italy.
Marino MM; Unit of Microbiology, The Great Romagna Hub Laboratory, 47522 Pievesestina, Italy.
Grumiro L; Unit of Microbiology, The Great Romagna Hub Laboratory, 47522 Pievesestina, Italy.
Scalcione A; Unit of Microbiology, The Great Romagna Hub Laboratory, 47522 Pievesestina, Italy.
Turba ME; Xenturion Srl, 47122 Forlì, Italy.
Gentilini F; Department of Veterinary Medical Sciences, Alma Mater Studiorum-University of Bologna, 40064 Bologna, Italy.
Fantini M; Unit of Microbiology, The Great Romagna Hub Laboratory, 47522 Pievesestina, Italy.
Zannoli S; Unit of Microbiology, The Great Romagna Hub Laboratory, 47522 Pievesestina, Italy.
Dirani G; Unit of Microbiology, The Great Romagna Hub Laboratory, 47522 Pievesestina, Italy.
Sambri V; Unit of Microbiology, The Great Romagna Hub Laboratory, 47522 Pievesestina, Italy.; Department of Experimental, Diagnostic and Specialty Medicine-DIMES, Alma Mater Studiorum-University of Bologna, 40138 Bologna, Italy.
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Źródło :
Viruses [Viruses] 2021 May 28; Vol. 13 (6). Date of Electronic Publication: 2021 May 28.
Typ publikacji :
Comparative Study; Journal Article
MeSH Terms :
Reverse Transcriptase Polymerase Chain Reaction*
COVID-19/*therapy
COVID-19/*virology
Polymerase Chain Reaction/*methods
RNA, Viral/*analysis
SARS-CoV-2/*isolation & purification
Viral Load/*methods
Animals ; Cells, Cultured ; Chlorocebus aethiops ; Digital Technology/methods ; Humans ; SARS-CoV-2/genetics ; Vero Cells ; Virus Cultivation
Czasopismo naukowe
Tytuł :
A high leukocyte count and administration of hydrocortisone hamper PCR-based diagnostics for bloodstream infections.
Autorzy :
Huber S; Institute of Hygiene and Medical Microbiology, Medical University of Innsbruck, Schöpfstrasse 41, 6020, Innsbruck, Austria.
Weinberger J; Ares Genetics GmbH, Karl-Farkas-Gasse 18, 1030, Vienna, Austria.
Pilecky M; Center for Biomedical Technology, Department for Biomedical Research, Danube University Krems, Krems, Austria.
Lorenz I; Department of General and Surgical Critical Care Medicine, Medical University of Innsbruck, Innsbruck, Austria.
Schildberger A; Center for Biomedical Technology, Department for Biomedical Research, Danube University Krems, Krems, Austria.
Weber V; Center for Biomedical Technology, Department for Biomedical Research, Danube University Krems, Krems, Austria.; Christian Doppler Laboratory for Innovative Therapy Approaches in Sepsis, Danube University Krems, Krems, Austria.
Fuchs S; Institute of Hygiene and Medical Microbiology, Medical University of Innsbruck, Schöpfstrasse 41, 6020, Innsbruck, Austria.
Posch W; Institute of Hygiene and Medical Microbiology, Medical University of Innsbruck, Schöpfstrasse 41, 6020, Innsbruck, Austria.
Knabl L; Institute of Hygiene and Medical Microbiology, Medical University of Innsbruck, Schöpfstrasse 41, 6020, Innsbruck, Austria.
Würzner R; Institute of Hygiene and Medical Microbiology, Medical University of Innsbruck, Schöpfstrasse 41, 6020, Innsbruck, Austria.
Posch AE; Ares Genetics GmbH, Karl-Farkas-Gasse 18, 1030, Vienna, Austria. .
Orth-Höller D; Institute of Hygiene and Medical Microbiology, Medical University of Innsbruck, Schöpfstrasse 41, 6020, Innsbruck, Austria. .
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Źródło :
European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology [Eur J Clin Microbiol Infect Dis] 2021 Jul; Vol. 40 (7), pp. 1441-1449. Date of Electronic Publication: 2021 Feb 05.
Typ publikacji :
Journal Article
MeSH Terms :
Hydrocortisone/*administration & dosage
Molecular Diagnostic Techniques/*methods
Polymerase Chain Reaction/*methods
Sepsis/*microbiology
Adolescent ; Adult ; Aged ; Blood Culture/methods ; Child ; Child, Preschool ; Female ; Humans ; Infant ; Infant, Newborn ; Leukocyte Count ; Male ; Middle Aged ; Multiplex Polymerase Chain Reaction ; Retrospective Studies ; Sensitivity and Specificity ; Young Adult
Czasopismo naukowe
Tytuł :
Comparison of real-time and droplet digital PCR to detect and quantify SARS-CoV-2 RNA in plasma.
Autorzy :
Tedim AP; Group for Biomedical Research in Sepsis (BioSepsis), Instituto de Investigación Biomédica de Salamanca, Salamanca, Spain.; Hospital Universitario Río Hortega, Valladolid, Spain.
Almansa R; Group for Biomedical Research in Sepsis (BioSepsis), Instituto de Investigación Biomédica de Salamanca, Salamanca, Spain.; Hospital Universitario Río Hortega, Valladolid, Spain.; Centro de Investigación Biomédica en Red en Enfermedades Respiratorias (CIBERES), Instituto de Salud Carlos III, Av. de Monforte de Lemos, Madrid, Spain.
Domínguez-Gil M; Microbiology Service, Hospital Universitario Rio Hortega, Valladolid, Spain.
González-Rivera M; Department of Laboratory Medicine, Hospital General Universitario Gregorio Marañón, Madrid, Spain.
Micheloud D; Emergency Department, Hospital General Universitario Gregorio Marañón, Madrid, Spain.
Ryan P; Hospital Universitario Infanta Leonor, Madrid, Spain.
Méndez R; Pulmonology Service, Hospital Universitario y Politécnico de La Fe, Avinguda de Fernando Abril Martorell, Spain.
Blanca-López N; Hospital Universitario Infanta Leonor, Madrid, Spain.
Pérez-García F; Servicio de Microbiología Clínica, Hospital Universitario Príncipe de Asturias, Madrid, Spain.
Bustamante E; Intensive Care Unit, Hospital Clínico Universitario de Valladolid. Av. Ramón y Cajal, Valladolid, Spain.
Gómez JM; Intensive Care Unit. Hospital General Universitario Gregorio Marañón, Madrid, Spain.
Doncel C; Group for Biomedical Research in Sepsis (BioSepsis), Instituto de Investigación Biomédica de Salamanca, Salamanca, Spain.; Hospital Universitario Río Hortega, Valladolid, Spain.; Centro de Investigación Biomédica en Red en Enfermedades Respiratorias (CIBERES), Instituto de Salud Carlos III, Av. de Monforte de Lemos, Madrid, Spain.
Trapiello W; Clinical Analysis Service. Hospital, Clínico Universitario de Valladolid, Av. Ramón y Cajal, Valladolid, Spain.
Kelvin AA; Department of Microbiology and Immunology, Faculty of Medicine, Canadian Center for Vaccinology CCfV, Dalhousie University, Halifax, Nova Scotia, Canada.; Laboratory of Immunity, Shantou University Medical College, Jinping, Shantou, China.
Booth R; Department of Microbiology and Immunology, Faculty of Medicine, Canadian Center for Vaccinology CCfV, Dalhousie University, Halifax, Nova Scotia, Canada.; Laboratory of Immunity, Shantou University Medical College, Jinping, Shantou, China.
Ostadgavahi AT; Department of Microbiology and Immunology, Faculty of Medicine, Canadian Center for Vaccinology CCfV, Dalhousie University, Halifax, Nova Scotia, Canada.; Laboratory of Immunity, Shantou University Medical College, Jinping, Shantou, China.
Oneizat R; Microbiology Service, Hospital Universitario Rio Hortega, Valladolid, Spain.
Puertas C; Department of Laboratory Medicine, Hospital General Universitario Gregorio Marañón, Madrid, Spain.
Barbé F; Centro de Investigación Biomédica en Red en Enfermedades Respiratorias (CIBERES), Instituto de Salud Carlos III, Av. de Monforte de Lemos, Madrid, Spain.; Respiratory Department, Institut Ricerca Biomedica de Lleida, Av. Alcalde Rovira Roure, Lleida, Spain.
Ferrer R; Centro de Investigación Biomédica en Red en Enfermedades Respiratorias (CIBERES), Instituto de Salud Carlos III, Av. de Monforte de Lemos, Madrid, Spain.; Intensive Care Department, SODIR Research Group, Vall d'Hebron Hospital Universitari, Vall d'Hebron Institut de Recerca, Barcelona, Spain.
Menéndez R; Centro de Investigación Biomédica en Red en Enfermedades Respiratorias (CIBERES), Instituto de Salud Carlos III, Av. de Monforte de Lemos, Madrid, Spain.; Pulmonology Service, Hospital Universitario y Politécnico de La Fe, Avinguda de Fernando Abril Martorell, Spain.
Bermejo-Martin JF; Group for Biomedical Research in Sepsis (BioSepsis), Instituto de Investigación Biomédica de Salamanca, Salamanca, Spain.; Hospital Universitario Río Hortega, Valladolid, Spain.; Centro de Investigación Biomédica en Red en Enfermedades Respiratorias (CIBERES), Instituto de Salud Carlos III, Av. de Monforte de Lemos, Madrid, Spain.
Eiros JM; Microbiology Service, Hospital Universitario Rio Hortega, Valladolid, Spain.
Kelvin DJ; Department of Microbiology and Immunology, Faculty of Medicine, Canadian Center for Vaccinology CCfV, Dalhousie University, Halifax, Nova Scotia, Canada.; Laboratory of Immunity, Shantou University Medical College, Jinping, Shantou, China.
Torres A; Centro de Investigación Biomédica en Red en Enfermedades Respiratorias (CIBERES), Instituto de Salud Carlos III, Av. de Monforte de Lemos, Madrid, Spain.; Department of Pulmonology, Institut D investigacions August Pi I Sunyer (IDIBAPS), Hospital Clinic de Barcelona, Universidad de Barcelona, Barcelona, Spain.
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Źródło :
European journal of clinical investigation [Eur J Clin Invest] 2021 Jun; Vol. 51 (6), pp. e13501. Date of Electronic Publication: 2021 Feb 08.
Typ publikacji :
Comparative Study; Journal Article
MeSH Terms :
COVID-19/*blood
RNA, Viral/*blood
Real-Time Polymerase Chain Reaction/*methods
Aged ; Ambulatory Care ; Female ; Humans ; Intensive Care Units ; Male ; Middle Aged ; Patients' Rooms ; Polymerase Chain Reaction/methods ; SARS-CoV-2/genetics ; Severity of Illness Index
Czasopismo naukowe
Tytuł :
An Eye on COVID-19: A Meta-analysis of Positive Conjunctival Reverse Transcriptase-Polymerase Chain Reaction and SARS-CoV-2 Conjunctivitis Prevalence.
Autorzy :
Sopp NM
Sharda V; Atrius Health, Newton, Massachusetts.
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Źródło :
Optometry and vision science : official publication of the American Academy of Optometry [Optom Vis Sci] 2021 May 01; Vol. 98 (5), pp. 429-436.
Typ publikacji :
Journal Article; Meta-Analysis; Review
MeSH Terms :
COVID-19/*epidemiology
Conjunctivitis/*epidemiology
Reverse Transcriptase Polymerase Chain Reaction/*methods
Angiotensin-Converting Enzyme 2/metabolism ; COVID-19/complications ; COVID-19 Nucleic Acid Testing ; Conjunctiva/virology ; Conjunctivitis/complications ; Conjunctivitis/virology ; Humans ; Pandemics ; Polymerase Chain Reaction ; Prevalence ; RNA, Viral/analysis ; Tears/chemistry ; Tears/virology
Czasopismo naukowe
Tytuł :
Evaluation of three extraction-free SARS-CoV-2 RT-PCR assays: A feasible alternative approach with low technical requirements.
Autorzy :
Visseaux B; Université de Paris, INSERM UMR 1137 IAME, Laboratoire de Virologie, AP-HP, Hôpital Bichat-Claude Bernard, F-75018 Paris, France.
Collin G; Université de Paris, INSERM UMR 1137 IAME, Laboratoire de Virologie, AP-HP, Hôpital Bichat-Claude Bernard, F-75018 Paris, France.
Houhou-Fidouh N; Université de Paris, INSERM UMR 1137 IAME, Laboratoire de Virologie, AP-HP, Hôpital Bichat-Claude Bernard, F-75018 Paris, France.
Le Hingrat Q; Université de Paris, INSERM UMR 1137 IAME, Laboratoire de Virologie, AP-HP, Hôpital Bichat-Claude Bernard, F-75018 Paris, France.
Ferré VM; Université de Paris, INSERM UMR 1137 IAME, Laboratoire de Virologie, AP-HP, Hôpital Bichat-Claude Bernard, F-75018 Paris, France.
Damond F; Université de Paris, INSERM UMR 1137 IAME, Laboratoire de Virologie, AP-HP, Hôpital Bichat-Claude Bernard, F-75018 Paris, France.
Ichou H; Université de Paris, INSERM UMR 1137 IAME, Laboratoire de Virologie, AP-HP, Hôpital Bichat-Claude Bernard, F-75018 Paris, France.
Descamps D; Université de Paris, INSERM UMR 1137 IAME, Laboratoire de Virologie, AP-HP, Hôpital Bichat-Claude Bernard, F-75018 Paris, France.
Charpentier C; Université de Paris, INSERM UMR 1137 IAME, Laboratoire de Virologie, AP-HP, Hôpital Bichat-Claude Bernard, F-75018 Paris, France. Electronic address: .
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Źródło :
Journal of virological methods [J Virol Methods] 2021 May; Vol. 291, pp. 114086. Date of Electronic Publication: 2021 Feb 09.
Typ publikacji :
Journal Article
MeSH Terms :
COVID-19/*diagnosis
COVID-19 Testing/*methods
Reverse Transcriptase Polymerase Chain Reaction/*methods
SARS-CoV-2/*genetics
SARS-CoV-2/*isolation & purification
COVID-19/virology ; Clinical Laboratory Techniques/methods ; RNA, Viral/genetics ; Reagent Kits, Diagnostic ; Real-Time Polymerase Chain Reaction/methods ; Sensitivity and Specificity ; Viral Load
Czasopismo naukowe
Tytuł :
Highly sensitive detection of SARS-CoV-2 RNA by multiplex rRT-PCR for molecular diagnosis of COVID-19 by clinical laboratories.
Autorzy :
Ishige T; Division of Laboratory Medicine, Chiba University Hospital, 1-8-1 Inohana, Chuo-ward, Chiba-city, Chiba 266-8677, Japan. Electronic address: .
Murata S; Division of Laboratory Medicine, Chiba University Hospital, 1-8-1 Inohana, Chuo-ward, Chiba-city, Chiba 266-8677, Japan.
Taniguchi T; Department of Infectious Diseases, Chiba University Hospital, 1-8-1 Inohana, Chuo-ward, Chiba-city, Chiba 266-8677, Japan.
Miyabe A; Division of Laboratory Medicine, Chiba University Hospital, 1-8-1 Inohana, Chuo-ward, Chiba-city, Chiba 266-8677, Japan.
Kitamura K; Division of Laboratory Medicine, Chiba University Hospital, 1-8-1 Inohana, Chuo-ward, Chiba-city, Chiba 266-8677, Japan.
Kawasaki K; Division of Laboratory Medicine, Chiba University Hospital, 1-8-1 Inohana, Chuo-ward, Chiba-city, Chiba 266-8677, Japan.
Nishimura M; Division of Laboratory Medicine, Chiba University Hospital, 1-8-1 Inohana, Chuo-ward, Chiba-city, Chiba 266-8677, Japan.
Igari H; Department of Infectious Diseases, Chiba University Hospital, 1-8-1 Inohana, Chuo-ward, Chiba-city, Chiba 266-8677, Japan.
Matsushita K; Division of Laboratory Medicine, Chiba University Hospital, 1-8-1 Inohana, Chuo-ward, Chiba-city, Chiba 266-8677, Japan.
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Źródło :
Clinica chimica acta; international journal of clinical chemistry [Clin Chim Acta] 2020 Aug; Vol. 507, pp. 139-142. Date of Electronic Publication: 2020 Apr 23.
Typ publikacji :
Journal Article
MeSH Terms :
Multiplex Polymerase Chain Reaction*
Reverse Transcriptase Polymerase Chain Reaction*/methods
Reverse Transcriptase Polymerase Chain Reaction*/standards
Betacoronavirus/*genetics
Clinical Laboratory Techniques/*methods
Coronavirus Infections/*diagnosis
Coronavirus Infections/*genetics
Pneumonia, Viral/*diagnosis
RNA, Viral/*genetics
Betacoronavirus/isolation & purification ; COVID-19 ; COVID-19 Testing ; COVID-19 Vaccines ; Clinical Laboratory Techniques/standards ; Humans ; Pandemics ; Pneumonia, Viral/genetics ; RNA, Viral/isolation & purification ; Real-Time Polymerase Chain Reaction/methods ; Real-Time Polymerase Chain Reaction/standards ; Reproducibility of Results ; SARS-CoV-2 ; Sputum/chemistry ; Sputum/virology
Czasopismo naukowe
Tytuł :
Sensitive detection and quantification of SARS-CoV-2 by multiplex droplet digital RT-PCR.
Autorzy :
de Kock R; Clinical Laboratory, Catharina Hospital Eindhoven, Eindhoven, The Netherlands. .; Institute for Complex Molecular Systems and Department of Biomedical Engineering, Laboratory of Chemical Biology, Eindhoven University of Technology, Eindhoven, The Netherlands. .; Expert Center Clinical Chemistry Eindhoven, Eindhoven, The Netherlands. .
Baselmans M; Clinical Laboratory, Catharina Hospital Eindhoven, Eindhoven, The Netherlands.
Scharnhorst V; Clinical Laboratory, Catharina Hospital Eindhoven, Eindhoven, The Netherlands.; Institute for Complex Molecular Systems and Department of Biomedical Engineering, Laboratory of Chemical Biology, Eindhoven University of Technology, Eindhoven, The Netherlands.; Expert Center Clinical Chemistry Eindhoven, Eindhoven, The Netherlands.
Deiman B; Clinical Laboratory, Catharina Hospital Eindhoven, Eindhoven, The Netherlands.; Institute for Complex Molecular Systems and Department of Biomedical Engineering, Laboratory of Chemical Biology, Eindhoven University of Technology, Eindhoven, The Netherlands.; Expert Center Clinical Chemistry Eindhoven, Eindhoven, The Netherlands.
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Źródło :
European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology [Eur J Clin Microbiol Infect Dis] 2021 Apr; Vol. 40 (4), pp. 807-813. Date of Electronic Publication: 2020 Oct 26.
Typ publikacji :
Journal Article
MeSH Terms :
COVID-19/*diagnosis
COVID-19 Nucleic Acid Testing/*methods
DNA/*analysis
Multiplex Polymerase Chain Reaction/*methods
RNA, Messenger/*analysis
RNA, Viral/*analysis
Reverse Transcriptase Polymerase Chain Reaction/*methods
Autoantigens/genetics ; Coronavirus Envelope Proteins/genetics ; Coronavirus Nucleocapsid Proteins/genetics ; Coronavirus RNA-Dependent RNA Polymerase/genetics ; Genes, Essential ; Glucuronidase/genetics ; Humans ; Phosphoproteins/genetics ; Real-Time Polymerase Chain Reaction ; Ribonuclease P/genetics ; SARS-CoV-2 ; Sensitivity and Specificity
Czasopismo naukowe
Tytuł :
Noninvasive sexing of human preimplantation embryos using RT-PCR in the spent culture media: A proof-of-concept study.
Autorzy :
Esmaeili M; Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran; Department of Genetics, Faculty of Basic Sciences and Advanced Technologies in Biology, Department of Genetics, University of Science and Culture, ACECR, Tehran, Iran.
Bazrgar M; Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran. Electronic address: .
Gourabi H; Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
Ebrahimi B; Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
Boroujeni PB; Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
Fakhri M; Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
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Źródło :
European journal of obstetrics, gynecology, and reproductive biology [Eur J Obstet Gynecol Reprod Biol] 2020 Sep; Vol. 252, pp. 89-93. Date of Electronic Publication: 2020 Jun 12.
Typ publikacji :
Journal Article
MeSH Terms :
Blastocyst*
Reverse Transcriptase Polymerase Chain Reaction*
Sex Determination Analysis*
Culture Media ; Female ; Humans ; In Situ Hybridization, Fluorescence ; Male ; Polymerase Chain Reaction ; Pregnancy
Czasopismo naukowe

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