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Tytuł:
Optimized MLPA workflow for spinal muscular atrophy diagnosis: identification of a novel variant, NC_000005.10:g.(70919941_70927324)del in isolated exon 1 of SMN1 gene through long-range PCR.
Autorzy:
Yao M; Department of Neurology, Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, Hangzhou, 310052, China.; Department of Infectious Diseases, Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, Hangzhou, China.
Jiang L; Department of Neurology, Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, Hangzhou, 310052, China.
Yu Y; Department of Neurology, Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, Hangzhou, 310052, China.
Cui Y; Department of Neurology, Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, Hangzhou, 310052, China.
Chen Y; Xiamen Biofast Biotechnology Co., Ltd., Xiamen, China.
Zhou D; Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, Hangzhou, China.
Gao F; Department of Neurology, Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, Hangzhou, 310052, China.
Mao S; Department of Neurology, Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, Hangzhou, 310052, China. .
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Źródło:
BMC neurology [BMC Neurol] 2024 Mar 11; Vol. 24 (1), pp. 93. Date of Electronic Publication: 2024 Mar 11.
Typ publikacji:
Journal Article
MeSH Terms:
Multiplex Polymerase Chain Reaction*
Muscular Atrophy, Spinal*/diagnosis
Muscular Atrophy, Spinal*/genetics
Humans ; DNA Copy Number Variations/genetics ; Workflow ; Motor Neurons ; Exons/genetics ; Survival of Motor Neuron 1 Protein/genetics
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknie Pełny tekstSzczegóły
Tytuł:
Identification of two novel large deletions in FBN1 gene by next-generation sequencing and multiplex ligation-dependent probe amplification.
Autorzy:
Lu X; Center of Clinical Laboratory, Zhongshan Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, 361004, China.
Wang R; Department of Cardiovascular Surgery, Fujian Provincial Hospital, Fuzhou, 350001, Fujian, China.
Li M; Department of Clinical Laboratory, Fujian Provincial Hospital, Fuzhou, 350001, Fujian, China.
Zhang B; Department of Clinical Laboratory, Fujian Provincial Hospital, Fuzhou, 350001, Fujian, China.
Rao H; Department of Ophthalmology, Fujian Provincial Hospital, Fuzhou, 350001, Fujian, China.
Huang X; Department of Clinical Laboratory, Fujian Provincial Hospital, Fuzhou, 350001, Fujian, China.
Chen X; Department of Clinical Laboratory, Fujian Provincial Hospital, Fuzhou, 350001, Fujian, China.
Wu Y; Department of Clinical Laboratory, Fujian Provincial Hospital, Fuzhou, 350001, Fujian, China. .
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Źródło:
BMC medical genomics [BMC Med Genomics] 2024 Feb 05; Vol. 17 (1), pp. 47. Date of Electronic Publication: 2024 Feb 05.
Typ publikacji:
Journal Article
MeSH Terms:
Multiplex Polymerase Chain Reaction*
Marfan Syndrome*/genetics
Marfan Syndrome*/diagnosis
Humans ; Genetic Testing ; Mutation ; High-Throughput Nucleotide Sequencing ; Fibrillin-1/genetics ; Adipokines/genetics
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknie Pełny tekstSzczegóły
Tytuł:
primerJinn: a tool for rationally designing multiplex PCR primer sets for amplicon sequencing and performing in silico PCR.
Autorzy:
Limberis JD; Division of Pulmonary and Critical Care Medicine, Trauma Centre, Zuckerberg San Francisco General Hospital, University of California, San Francisco, San Francisco, CA, USA. .
Metcalfe JZ; Division of Pulmonary and Critical Care Medicine, Trauma Centre, Zuckerberg San Francisco General Hospital, University of California, San Francisco, San Francisco, CA, USA.
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Źródło:
BMC bioinformatics [BMC Bioinformatics] 2023 Dec 12; Vol. 24 (1), pp. 468. Date of Electronic Publication: 2023 Dec 12.
Typ publikacji:
Journal Article
MeSH Terms:
Multiplex Polymerase Chain Reaction*/methods
Mycobacterium tuberculosis*/genetics
DNA Primers/genetics ; Sequence Analysis ; Base Sequence
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknie Pełny tekstSzczegóły
Tytuł:
Prenatal diagnosis of 1408 foetuses at risk of DMD/BMD by MLPA and Sanger sequencing combined with STR linkage analysis.
Autorzy:
Hua C; Genetics and Prenatal Diagnosis Center, Department of Obstetrics and Gynaecology, The First Affiliated Hospital of Zhengzhou University, 450052, Zhengzhou, China.
Liu L; Genetics and Prenatal Diagnosis Center, Department of Obstetrics and Gynaecology, The First Affiliated Hospital of Zhengzhou University, 450052, Zhengzhou, China.
Kong X; Genetics and Prenatal Diagnosis Center, Department of Obstetrics and Gynaecology, The First Affiliated Hospital of Zhengzhou University, 450052, Zhengzhou, China. .
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Źródło:
BMC medical genomics [BMC Med Genomics] 2023 Dec 01; Vol. 16 (1), pp. 310. Date of Electronic Publication: 2023 Dec 01.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
Multiplex Polymerase Chain Reaction*
Muscular Dystrophy, Duchenne*/diagnosis
Muscular Dystrophy, Duchenne*/genetics
Humans ; Male ; Female ; Pregnancy ; Retrospective Studies ; Exons ; Prenatal Diagnosis/methods ; Fetus
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknie Pełny tekstSzczegóły
Tytuł:
Simple, Precise, and Less Biased GMO Quantification by Multiplexed Genetic Element-Specific Digital PCR.
Autorzy:
Noma S; Research Center for Basic Science, Nisshin Seifun Group Inc, 5-3-1 Tsurugaoka, Fujimino, Saitama 356-8511, Japan.
Kikuchi Y; Research Center for Basic Science, Nisshin Seifun Group Inc, 5-3-1 Tsurugaoka, Fujimino, Saitama 356-8511, Japan.
Satou M; Food Research Center, Nippn Corporation, 5-1-3 Midorigaoka, Atsugi, Kanagawa 243-0041, Japan.
Tanaka T; Food Research Center, Nippn Corporation, 5-1-3 Midorigaoka, Atsugi, Kanagawa 243-0041, Japan.
Takiya T; Food Research Center, Nippn Corporation, 5-1-3 Midorigaoka, Atsugi, Kanagawa 243-0041, Japan.
Okusu H; Food Research Center, Nippn Corporation, 5-1-3 Midorigaoka, Atsugi, Kanagawa 243-0041, Japan.
Futo S; FASMAC Co., Ltd, 5-1-3 Midorigaoka, Atsugi, Kanagawa 243-0041, Japan.
Takabatake R; Institute of Food Research, National Agriculture and Food Research Organization, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan.
Kitta K; Institute of Food Research, National Agriculture and Food Research Organization, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan.
Mano J; Institute of Food Research, National Agriculture and Food Research Organization, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan.
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Źródło:
Journal of AOAC International [J AOAC Int] 2022 Feb 04; Vol. 105 (1), pp. 159-166.
Typ publikacji:
Journal Article
MeSH Terms:
Multiplex Polymerase Chain Reaction*
Zea mays*/genetics
DNA ; DNA, Plant/genetics ; Plants, Genetically Modified/genetics ; Real-Time Polymerase Chain Reaction
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknieSzczegóły
Tytuł:
Comparison of FACS and PCR for Detection of BCMA-CAR-T Cells.
Autorzy:
Reichman A; Department of Hematology, Oncology and Rheumatology, Heidelberg University Hospital, Im Neuenheimer Feld 410, 69120 Heidelberg, Germany.
Kunz A; Department of Hematology, Oncology and Rheumatology, Heidelberg University Hospital, Im Neuenheimer Feld 410, 69120 Heidelberg, Germany.
Joedicke JJ; Department of Microenvironmental Regulation in Autoimmunity and Cancer, Max-Delbrück Center for Molecular Medicine (MDC), Robert-Rössle-Str. 10, 13125 Berlin-Buch, Germany.
Höpken UE; Department of Translational Tumor Immunology, Max-Delbrück Center for Molecular Medicine (MDC), Robert-Rössle-Str. 10, 13125 Berlin-Buch, Germany.
Keib A; Department of Hematology, Oncology and Rheumatology, Heidelberg University Hospital, Im Neuenheimer Feld 410, 69120 Heidelberg, Germany.
Neuber B; Department of Hematology, Oncology and Rheumatology, Heidelberg University Hospital, Im Neuenheimer Feld 410, 69120 Heidelberg, Germany.
Sedloev D; Department of Hematology, Oncology and Rheumatology, Heidelberg University Hospital, Im Neuenheimer Feld 410, 69120 Heidelberg, Germany.
Wang L; Department of Hematology, Oncology and Rheumatology, Heidelberg University Hospital, Im Neuenheimer Feld 410, 69120 Heidelberg, Germany.
Jiang G; Department of Hematology, Oncology and Rheumatology, Heidelberg University Hospital, Im Neuenheimer Feld 410, 69120 Heidelberg, Germany.
Hückelhoven-Krauss A; Department of Hematology, Oncology and Rheumatology, Heidelberg University Hospital, Im Neuenheimer Feld 410, 69120 Heidelberg, Germany.
Eberhardt F; Department of Hematology, Oncology and Rheumatology, Heidelberg University Hospital, Im Neuenheimer Feld 410, 69120 Heidelberg, Germany.
Müller-Tidow C; Department of Hematology, Oncology and Rheumatology, Heidelberg University Hospital, Im Neuenheimer Feld 410, 69120 Heidelberg, Germany.
Wermke M; NCT/UCC Early Clinical Trial Unit (ECTU), Medical Faculty C.-G. Carus, Technical University Dresden, Fetscherstraße 74, 01307 Dresden, Germany.
Rehm A; Department of Microenvironmental Regulation in Autoimmunity and Cancer, Max-Delbrück Center for Molecular Medicine (MDC), Robert-Rössle-Str. 10, 13125 Berlin-Buch, Germany.
Schmitt M; Department of Hematology, Oncology and Rheumatology, Heidelberg University Hospital, Im Neuenheimer Feld 410, 69120 Heidelberg, Germany.
Schmitt A; Department of Hematology, Oncology and Rheumatology, Heidelberg University Hospital, Im Neuenheimer Feld 410, 69120 Heidelberg, Germany.
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Źródło:
International journal of molecular sciences [Int J Mol Sci] 2022 Jan 14; Vol. 23 (2). Date of Electronic Publication: 2022 Jan 14.
Typ publikacji:
Journal Article
MeSH Terms:
Flow Cytometry*/methods
Flow Cytometry*/standards
Polymerase Chain Reaction*/methods
Polymerase Chain Reaction*/standards
B-Cell Maturation Antigen/*metabolism
Receptors, Chimeric Antigen/*metabolism
T-Lymphocytes/*metabolism
B-Cell Maturation Antigen/genetics ; Biomarkers ; Humans ; Immunophenotyping ; Immunotherapy, Adoptive/methods ; Immunotherapy, Adoptive/standards ; Real-Time Polymerase Chain Reaction ; Receptors, Chimeric Antigen/genetics ; Reproducibility of Results ; Sensitivity and Specificity ; T-Lymphocytes/immunology
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknie Pełny tekstSzczegóły
Tytuł:
Processing Hundreds of SARS-CoV-2 Samples with an In-House PCR-Based Method without Robotics.
Autorzy:
Mair T; Institute of Biophysics, Johannes Kepler University, 4020 Linz, Austria.
Ivankovic M; Institute of Biophysics, Johannes Kepler University, 4020 Linz, Austria.
Paar C; Institute of Laboratory Medicine, Johannes Kepler Universitätsklinikum, 4020 Linz, Austria.
Salzer HJF; Department of Pulmonology, Johannes Kepler Universitätsklinikum, 4020 Linz, Austria.
Heissl A; Department of Biosciences, University of Salzburg, 5020 Salzburg, Austria.
Lamprecht B; Department of Pulmonology, Johannes Kepler Universitätsklinikum, 4020 Linz, Austria.
Schreier-Lechner E; Institute of Laboratory Medicine, Johannes Kepler Universitätsklinikum, 4020 Linz, Austria.
Tiemann-Boege I; Institute of Biophysics, Johannes Kepler University, 4020 Linz, Austria.
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Źródło:
Viruses [Viruses] 2021 Aug 28; Vol. 13 (9). Date of Electronic Publication: 2021 Aug 28.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
Polymerase Chain Reaction*/methods
Polymerase Chain Reaction*/standards
SARS-CoV-2*/genetics
COVID-19/*diagnosis
COVID-19/*virology
COVID-19 Nucleic Acid Testing/*methods
COVID-19 Nucleic Acid Testing/standards ; Humans ; Reagent Kits, Diagnostic ; Real-Time Polymerase Chain Reaction/methods ; Real-Time Polymerase Chain Reaction/standards ; Reproducibility of Results ; Sensitivity and Specificity ; Viral Load
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknie Pełny tekstSzczegóły
Tytuł:
Comparison of two multiplex PCR tests for common pathogen detection in hospitalized children with acute respiratory infection.
Autorzy:
Wang L; Institute of Pediatric Research, Children's Hospital of Hebei Province, Shijiazhuang 050031, China.
Sun S; Institute of Pediatric Research, Children's Hospital of Hebei Province, Shijiazhuang 050031, China.
You D; Institute of Pediatric Research, Children's Hospital of Hebei Province, Shijiazhuang 050031, China.
Chen F; Institute of Pediatric Research, Children's Hospital of Hebei Province, Shijiazhuang 050031, China.
Guo Y; Institute of Pediatric Research, Children's Hospital of Hebei Province, Shijiazhuang 050031, China.
Zeng X; Ningbo Health Gene Tech Co., Ltd., Ningbo 315040, China.
Wang W; Ningbo Health Gene Tech Co., Ltd., Ningbo 315040, China.
Yang S; Institute of Pediatric Research, Children's Hospital of Hebei Province, Shijiazhuang 050031, China.
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Źródło:
Journal of infection in developing countries [J Infect Dev Ctries] 2023 Oct 31; Vol. 17 (10), pp. 1474-1479. Date of Electronic Publication: 2023 Oct 31.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
Multiplex Polymerase Chain Reaction*/methods
Respiratory Tract Infections*/diagnosis
Child ; Humans ; Infant ; Child, Hospitalized
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknieSzczegóły
Tytuł:
A tool to automatically design multiplex PCR primer pairs for specific targets using diverse templates.
Autorzy:
Yang L; Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518120, China.; State Key Laboratory of Resource Insects, Institute of Sericulture and Systems Biology, Southwest University, Chongqing, 400715, China.
Ding F; Shenzhen National Clinical Research Center for Infectious Diseases, No. 29, Bulan Road, Longgang District, Shenzhen, 518112, China.
Lin Q; Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518120, China.
Xie J; Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518120, China.; School of Life Sciences, Henan University, Kaifeng, 475004, China.; Shenzhen Research Institute of Henan University, Shenzhen, 518000, China.
Fan W; Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518120, China.
Dai F; State Key Laboratory of Resource Insects, Institute of Sericulture and Systems Biology, Southwest University, Chongqing, 400715, China. .
Cui P; Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518120, China. .
Liu W; Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518120, China. .
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Źródło:
Scientific reports [Sci Rep] 2023 Sep 30; Vol. 13 (1), pp. 16451. Date of Electronic Publication: 2023 Sep 30.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
Multiplex Polymerase Chain Reaction*
Phylogeny
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknie Pełny tekstSzczegóły
Tytuł:
Development and Implementation of a Quadruple RT-qPCR Method for the Identification of Porcine Reproductive and Respiratory Syndrome Virus Strains.
Autorzy:
Ruan S; National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.; The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430070, China.
Ren W; National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.; The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430070, China.
Yu B; National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.; The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430070, China.
Yu X; National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.; The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430070, China.
Wu H; National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.; The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430070, China.
Li W; National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.; The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430070, China.
Jiang Y; National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.; The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430070, China.
He Q; National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.; The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430070, China.
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Źródło:
Viruses [Viruses] 2023 Sep 18; Vol. 15 (9). Date of Electronic Publication: 2023 Sep 18.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
Porcine Reproductive and Respiratory Syndrome*/diagnosis
Porcine respiratory and reproductive syndrome virus*/genetics
Reverse Transcriptase Polymerase Chain Reaction*/veterinary
Animals ; Female ; Pregnancy ; China/epidemiology ; Sus scrofa ; Swine
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknie Pełny tekstSzczegóły
Tytuł:
Smart-Plexer: a breakthrough workflow for hybrid development of multiplex PCR assays.
Autorzy:
Miglietta L; Department of Infectious Disease, Faculty of Medicine, Imperial College London, London, UK.; Department of Electrical and Electronic Engineering, Faculty of Engineering, Imperial College London, London, UK.
Chen Y; Department of Electrical and Electronic Engineering, Faculty of Engineering, Imperial College London, London, UK.
Luo Z; Department of Infectious Disease, Faculty of Medicine, Imperial College London, London, UK.
Xu K; Department of Infectious Disease, Faculty of Medicine, Imperial College London, London, UK.; Department of Electrical and Electronic Engineering, Faculty of Engineering, Imperial College London, London, UK.
Ding N; Department of Infectious Disease, Faculty of Medicine, Imperial College London, London, UK.
Peng T; Department of Electrical and Electronic Engineering, Faculty of Engineering, Imperial College London, London, UK.
Moniri A; Department of Electrical and Electronic Engineering, Faculty of Engineering, Imperial College London, London, UK.
Kreitmann L; Department of Infectious Disease, Faculty of Medicine, Imperial College London, London, UK.
Cacho-Soblechero M; Department of Electrical and Electronic Engineering, Faculty of Engineering, Imperial College London, London, UK.
Holmes A; Department of Infectious Disease, Faculty of Medicine, Imperial College London, London, UK.
Georgiou P; Department of Electrical and Electronic Engineering, Faculty of Engineering, Imperial College London, London, UK.
Rodriguez-Manzano J; Department of Infectious Disease, Faculty of Medicine, Imperial College London, London, UK. .
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Źródło:
Communications biology [Commun Biol] 2023 Sep 09; Vol. 6 (1), pp. 922. Date of Electronic Publication: 2023 Sep 09.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
Multiplex Polymerase Chain Reaction*
Biological Assay*
Computer Simulation ; Workflow ; Kinetics
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknie Pełny tekstSzczegóły
Tytuł:
Development of Multiplex Polymerase Chain Reaction (PCR)-Based MSA Assay for Bladder Cancer Detection.
Autorzy:
Reynolds T; NEXT Bio-Research Services, LLC, 11601 Ironbridge Road, Suite 101, Chester, VA 23831, USA.
Gordon M; HJM Cancer Research Foundation Corporation, 10606 Candlewick Road, Lutherville, MD 21093, USA.; BCD Innovations USA, 10606 Candlewick Road, Lutherville, MD 21093, USA.
Monar GVF; HJM Cancer Research Foundation Corporation, 10606 Candlewick Road, Lutherville, MD 21093, USA.
Moon D; HJM Cancer Research Foundation Corporation, 10606 Candlewick Road, Lutherville, MD 21093, USA.
Moon C; HJM Cancer Research Foundation Corporation, 10606 Candlewick Road, Lutherville, MD 21093, USA.; BCD Innovations USA, 10606 Candlewick Road, Lutherville, MD 21093, USA.; Department of Otolaryngology-Head and Neck Surgery, The Johns Hopkins Medical Institution, Cancer Research Building II, 5M3, 1550 Orleans Street, Baltimore, MD 21205, USA.
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Źródło:
International journal of molecular sciences [Int J Mol Sci] 2023 Sep 04; Vol. 24 (17). Date of Electronic Publication: 2023 Sep 04.
Typ publikacji:
Journal Article
MeSH Terms:
Multiplex Polymerase Chain Reaction*
Urinary Bladder Neoplasms*/diagnosis
Urinary Bladder Neoplasms*/genetics
Humans ; Urinary Bladder ; Biological Assay ; Microsatellite Repeats/genetics
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknie Pełny tekstSzczegóły
Tytuł:
A Digital PCR Method Based on Highly Specific Taq for Detecting Gene Editing and Mutations.
Autorzy:
Li B; Shandong Provincial Key Laboratory of Animal Cell and Developmental Biology, School of Life Sciences, Shandong University, Qingdao 266237, China.
Liu J; Shandong Provincial Key Laboratory of Animal Cell and Developmental Biology, School of Life Sciences, Shandong University, Qingdao 266237, China.
Huang Q; Shandong Provincial Key Laboratory of Animal Cell and Developmental Biology, School of Life Sciences, Shandong University, Qingdao 266237, China.
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Źródło:
International journal of molecular sciences [Int J Mol Sci] 2023 Aug 29; Vol. 24 (17). Date of Electronic Publication: 2023 Aug 29.
Typ publikacji:
Journal Article
MeSH Terms:
Polymerase Chain Reaction*/methods
DNA Replication ; Fluorescent Dyes ; Gene Editing ; Mutation
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknie Pełny tekstSzczegóły
Tytuł:
Quadruplex Real-Time TaqMan RT-qPCR Assay for Differentiation of Equine Group A and B Rotaviruses and Identification of Group A G3 and G14 Genotypes.
Autorzy:
Carossino M; Louisiana Animal Disease Diagnostic Laboratory, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA.; Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA.
Balasuriya UBR; Louisiana Animal Disease Diagnostic Laboratory, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA.; Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA.
Thieulent CJ; Louisiana Animal Disease Diagnostic Laboratory, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA.; Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA.
Barrandeguy ME; Escuela de Veterinaria, Universidad del Salvador, Buenos Aires B1630, Argentina.; Instituto de Virología, CICVyA, Instituto Nacional de Tecnología Agropecuaria (INTA), Buenos Aires B1686, Argentina.
Vissani MA; Escuela de Veterinaria, Universidad del Salvador, Buenos Aires B1630, Argentina.; Instituto de Virología, CICVyA, Instituto Nacional de Tecnología Agropecuaria (INTA), Buenos Aires B1686, Argentina.; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires C1425, Argentina.
Parreño V; Instituto de Virología, CICVyA, Instituto Nacional de Tecnología Agropecuaria (INTA), Buenos Aires B1686, Argentina.; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires C1425, Argentina.
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Źródło:
Viruses [Viruses] 2023 Jul 26; Vol. 15 (8). Date of Electronic Publication: 2023 Jul 26.
Typ publikacji:
Evaluation Study; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, Non-U.S. Gov't
MeSH Terms:
Real-Time Polymerase Chain Reaction*/methods
Real-Time Polymerase Chain Reaction*/veterinary
Rotavirus*/isolation & purification
Horse Diseases*/virology
Rotavirus Infections*/veterinary
Animals ; Horses ; Feces/virology ; Sensitivity and Specificity
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknie Pełny tekstSzczegóły
Tytuł:
Drug Regulatory-Compliant Validation of a qPCR Assay for Bioanalysis Studies of a Cell Therapy Product with a Special Focus on Matrix Interferences in a Wide Range of Organ Tissues.
Autorzy:
Schröder HM; RHEACELL GmbH & Co. KG, 69120 Heidelberg, Germany.
Niebergall-Roth E; RHEACELL GmbH & Co. KG, 69120 Heidelberg, Germany.
Norrick A; RHEACELL GmbH & Co. KG, 69120 Heidelberg, Germany.
Esterlechner J; RHEACELL GmbH & Co. KG, 69120 Heidelberg, Germany.
Ganss C; RHEACELL GmbH & Co. KG, 69120 Heidelberg, Germany.
Frank MH; Department of Dermatology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.; Harvard Stem Cell Institute, Harvard University, Cambridge, MA 02138, USA.; Transplant Research Program, Boston Children's Hospital, Harvard Medical School, Boston, MA 02115, USA.; School of Medical and Health Sciences, Edith Cowan University, Perth, WA 6027, Australia.
Kluth MA; RHEACELL GmbH & Co. KG, 69120 Heidelberg, Germany.
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Źródło:
Cells [Cells] 2023 Jul 05; Vol. 12 (13). Date of Electronic Publication: 2023 Jul 05.
Typ publikacji:
Journal Article; Validation Study; Research Support, N.I.H., Extramural
MeSH Terms:
Mesenchymal Stem Cells*/metabolism
Polymerase Chain Reaction*/methods
Animals ; Humans ; Mice ; Transplantation, Heterologous
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknie Pełny tekstSzczegóły
Tytuł:
EndoGeneAnalyzer: A tool for selection and validation of reference genes.
Autorzy:
Teixeira EB; Oncology Research Center, Federal University of Pará, Belém, Pará, Brazil.
Khayat AS; Oncology Research Center, Federal University of Pará, Belém, Pará, Brazil.
Assumpção PP; Oncology Research Center, Federal University of Pará, Belém, Pará, Brazil.
Casseb SM; Oncology Research Center, Federal University of Pará, Belém, Pará, Brazil.
Moreira-Nunes CA; Oncology Research Center, Federal University of Pará, Belém, Pará, Brazil.; Pharmacogenetics Laboratory, Drug Research and Development Center (NPDM), Department of Medicine, Federal University of Ceará, Fortaleza, Brazil.; Clementino Fraga Group, Central Unity, Molecular Biology Laboratory, Fortaleza, CE, Brazil.
Moreira FC; Oncology Research Center, Federal University of Pará, Belém, Pará, Brazil.
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Źródło:
PloS one [PLoS One] 2024 Apr 03; Vol. 19 (4), pp. e0299993. Date of Electronic Publication: 2024 Apr 03 (Print Publication: 2024).
Typ publikacji:
Journal Article
MeSH Terms:
Gene Expression Profiling*
Real-Time Polymerase Chain Reaction ; Reference Standards ; Reverse Transcriptase Polymerase Chain Reaction
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknie Pełny tekstSzczegóły
Tytuł:
Detection of pathogenic Leptospira with rapid extraction followed by recombinase polymerase amplification (RPA) and quantitative polymerase chain reaction (qPCR) assay-A comprehensive study from Sri Lanka.
Autorzy:
Uduwawala H; Molecular Medicine Unit, Faculty of Medicine, University of Kelaniya, Ragama, Sri Lanka.
Manamperi A; Molecular Medicine Unit, Faculty of Medicine, University of Kelaniya, Ragama, Sri Lanka.
Gunaratna GPS; Department of Medical Microbiology, Faculty of Medicine, University of Kelaniya, Ragama, Sri Lanka.
Karunanayake L; National Reference Laboratory for Leptospirosis, Medical Research Institute, Colombo, Sri Lanka.
Ceruti A; Institute of Animal Hygiene and Veterinary Public Health, Leipzig University, Leipzig, Germany.
Abd El Wahed A; Institute of Animal Hygiene and Veterinary Public Health, Leipzig University, Leipzig, Germany.
Fernando L; Centre for Clinical Management of Dengue & Dengue Haemorrhagic Fever, District General Hospital, Negombo, Sri Lanka.
Premaratna R; Department of Medicine, Faculty of Medicine, University of Kelaniya, Ragama, Sri Lanka.
Hapugoda M; Molecular Medicine Unit, Faculty of Medicine, University of Kelaniya, Ragama, Sri Lanka.
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Źródło:
PloS one [PLoS One] 2024 Mar 15; Vol. 19 (3), pp. e0295287. Date of Electronic Publication: 2024 Mar 15 (Print Publication: 2024).
Typ publikacji:
Journal Article
MeSH Terms:
Leptospira*/genetics
Leptospirosis*/diagnosis
Animals ; Humans ; Recombinases ; Retrospective Studies ; Prospective Studies ; Sri Lanka ; Polymerase Chain Reaction ; Nucleotidyltransferases ; Zoonoses ; Sensitivity and Specificity ; Real-Time Polymerase Chain Reaction/methods ; Nucleic Acid Amplification Techniques/methods
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknie Pełny tekstSzczegóły
Tytuł:
Development and validation of multiplex real-time PCR for simultaneous detection of six bacterial pathogens causing lower respiratory tract infections and antimicrobial resistance genes.
Autorzy:
Dung TTN; Molecular Epidemiology Group, Oxford University Clinical Research Unit, 764 Vo Van Kiet Street, Ward 1, District 5, Ho Chi Minh City, Vietnam.
Phat VV; Molecular Epidemiology Group, Oxford University Clinical Research Unit, 764 Vo Van Kiet Street, Ward 1, District 5, Ho Chi Minh City, Vietnam.
Vinh C; Molecular Epidemiology Group, Oxford University Clinical Research Unit, 764 Vo Van Kiet Street, Ward 1, District 5, Ho Chi Minh City, Vietnam.
Lan NPH; Hospital for Tropical Diseases, Ho Chi Minh City, Vietnam.
Phuong NLN; Hospital for Tropical Diseases, Ho Chi Minh City, Vietnam.
Ngan LTQ; Hospital for Tropical Diseases, Ho Chi Minh City, Vietnam.
Thwaites G; Molecular Epidemiology Group, Oxford University Clinical Research Unit, 764 Vo Van Kiet Street, Ward 1, District 5, Ho Chi Minh City, Vietnam.; Centre for Tropical Medicine and Global Health, Nuffield Department of Clinical Medicine, Oxford University, Oxford, UK.
Thwaites L; Molecular Epidemiology Group, Oxford University Clinical Research Unit, 764 Vo Van Kiet Street, Ward 1, District 5, Ho Chi Minh City, Vietnam.; Centre for Tropical Medicine and Global Health, Nuffield Department of Clinical Medicine, Oxford University, Oxford, UK.
Rabaa M; Molecular Epidemiology Group, Oxford University Clinical Research Unit, 764 Vo Van Kiet Street, Ward 1, District 5, Ho Chi Minh City, Vietnam.; Centre for Tropical Medicine and Global Health, Nuffield Department of Clinical Medicine, Oxford University, Oxford, UK.
Nguyen ATK; Molecular Epidemiology Group, Oxford University Clinical Research Unit, 764 Vo Van Kiet Street, Ward 1, District 5, Ho Chi Minh City, Vietnam.; Hospital for Tropical Diseases, Ho Chi Minh City, Vietnam.
Duy PT; Molecular Epidemiology Group, Oxford University Clinical Research Unit, 764 Vo Van Kiet Street, Ward 1, District 5, Ho Chi Minh City, Vietnam. .; Centre for Tropical Medicine and Global Health, Nuffield Department of Clinical Medicine, Oxford University, Oxford, UK. .
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Źródło:
BMC infectious diseases [BMC Infect Dis] 2024 Feb 07; Vol. 24 (1), pp. 164. Date of Electronic Publication: 2024 Feb 07.
Typ publikacji:
Journal Article
MeSH Terms:
Anti-Bacterial Agents*/pharmacology
Anti-Bacterial Agents*/therapeutic use
Respiratory Tract Infections*/diagnosis
Respiratory Tract Infections*/microbiology
Humans ; Real-Time Polymerase Chain Reaction/methods ; Escherichia coli/genetics ; Staphylococcus aureus/genetics ; Reproducibility of Results ; Multiplex Polymerase Chain Reaction/methods ; Drug Resistance, Bacterial ; Bacteria/genetics ; Streptococcus pneumoniae/genetics ; Klebsiella pneumoniae/genetics
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknie Pełny tekstSzczegóły
Tytuł:
Identification of Torquetenovirus Species in Patients with Kawasaki Disease Using a Newly Developed Species-Specific PCR Method.
Autorzy:
Spezia PG; Department of Translational Research, University of Pisa, 56127 Pisa, Italy.
Filippini F; Department of Translational Research, University of Pisa, 56127 Pisa, Italy.
Nagao Y; Department of Paediatrics, Japan Community Health Care Organisation Osaka Hospital, Osaka 5530003, Japan.; Department of Paediatrics, Fukuoka Tokushukai Hospital, Fukuoka 8160864, Japan.
Sano T; Department of Paediatrics, Japan Community Health Care Organisation Osaka Hospital, Osaka 5530003, Japan.
Ishida T; Department of Biological Sciences, University of Tokyo, Tokyo 1130033, Japan.
Maggi F; Laboratory of Virology, National Institute for Infectious Diseases 'Lazzaro Spallanzani'-IRCCS, 00149 Rome, Italy.
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Źródło:
International journal of molecular sciences [Int J Mol Sci] 2023 May 12; Vol. 24 (10). Date of Electronic Publication: 2023 May 12.
Typ publikacji:
Journal Article
MeSH Terms:
Torque teno virus*/genetics
Torque teno virus*/isolation & purification
Mucocutaneous Lymph Node Syndrome*/virology
Polymerase Chain Reaction*/methods
DNA Virus Infections*/virology
High-Throughput Nucleotide Sequencing ; Datasets as Topic ; Humans ; Male ; Female ; Infant ; Child, Preschool ; Child ; Prospective Studies ; DNA, Viral/genetics ; DNA, Viral/isolation & purification
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknie Pełny tekstSzczegóły
Tytuł:
Neq2X7: a multi-purpose and open-source fusion DNA polymerase for advanced DNA engineering and diagnostics PCR.
Autorzy:
Hernández-Rollán C; The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Søltofts Plads, Building 220, Kongens Lyngby, 2800, Denmark.
Ehrmann AK; The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Søltofts Plads, Building 220, Kongens Lyngby, 2800, Denmark.
Vlassis A; The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Søltofts Plads, Building 220, Kongens Lyngby, 2800, Denmark.
Kandasamy V; The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Søltofts Plads, Building 220, Kongens Lyngby, 2800, Denmark.
Nørholm MHH; The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Søltofts Plads, Building 220, Kongens Lyngby, 2800, Denmark. .; Mycropt ApS, Kongens Lyngby, 2800, Denmark. .
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Źródło:
BMC biotechnology [BMC Biotechnol] 2024 Apr 02; Vol. 24 (1), pp. 17. Date of Electronic Publication: 2024 Apr 02.
Typ publikacji:
Journal Article
MeSH Terms:
DNA-Directed DNA Polymerase*/genetics
Uracil*/metabolism
Polymerase Chain Reaction ; Plasmids ; DNA
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknie Pełny tekstSzczegóły
Wyświetlanie 1-20 z 435928

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