Comparative functional genomics identifies an iron-limited bottleneck in a Saccharomyces cerevisiae strain with a cytosolic-localized isobutanol pathway.
Autorzy:
Gambacorta FV; DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, Madison, WI, USA.; Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, WI, USA. Wagner ER; DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, Madison, WI, USA.; Laboratory of Genetics, Center for Genomic Science Innovation, University of Wisconsin-Madison, Madison, WI, USA. Jacobson TB; DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, Madison, WI, USA.; Department of Bacteriology, University of Wisconsin-Madison, Madison, WI, USA. Tremaine M; DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, Madison, WI, USA. Muehlbauer LK; Department of Chemistry, University of Wisconsin-Madison, Madison, WI, USA. McGee MA; DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, Madison, WI, USA. Baerwald JJ; Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, WI, USA. Wrobel RL; DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, Madison, WI, USA.; Laboratory of Genetics, Center for Genomic Science Innovation, University of Wisconsin-Madison, Madison, WI, USA.; Wisconsin Energy Institute, J. F. Crow Institute for the Study of Evolution, University of Wisconsin-Madison, Madison, WI, USA. Wolters JF; DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, Madison, WI, USA.; Laboratory of Genetics, Center for Genomic Science Innovation, University of Wisconsin-Madison, Madison, WI, USA.; Wisconsin Energy Institute, J. F. Crow Institute for the Study of Evolution, University of Wisconsin-Madison, Madison, WI, USA. Place M; DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, Madison, WI, USA. Dietrich JJ; DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, Madison, WI, USA.; Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, WI, USA. Xie D; DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, Madison, WI, USA. Serate J; DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, Madison, WI, USA. Gajbhiye S; DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, Madison, WI, USA. Liu L; DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, Madison, WI, USA.; Department of Bacteriology, University of Wisconsin-Madison, Madison, WI, USA. Vang-Smith M; DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, Madison, WI, USA. Coon JJ; Department of Chemistry, University of Wisconsin-Madison, Madison, WI, USA.; Department of Biomolecular Chemistry, University of Wisconsin-Madison, Madison, WI, USA. Zhang Y; DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, Madison, WI, USA. Gasch AP; DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, Madison, WI, USA.; Laboratory of Genetics, Center for Genomic Science Innovation, University of Wisconsin-Madison, Madison, WI, USA. Amador-Noguez D; DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, Madison, WI, USA.; Department of Bacteriology, University of Wisconsin-Madison, Madison, WI, USA. Hittinger CT; DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, Madison, WI, USA.; Laboratory of Genetics, Center for Genomic Science Innovation, University of Wisconsin-Madison, Madison, WI, USA.; Wisconsin Energy Institute, J. F. Crow Institute for the Study of Evolution, University of Wisconsin-Madison, Madison, WI, USA. Sato TK; DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, Madison, WI, USA. PflegerBF; DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, Madison, WI, USA.; Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, WI, USA.
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Źródło:
Synthetic and systems biotechnology [Synth Syst Biotechnol] 2022 Mar 18; Vol. 7 (2), pp. 738-749. Date of Electronic Publication: 2022 Mar 18 (Print Publication: 2022).
Enabling commercial success of industrial biotechnology.
Autorzy:
Biggs BW; Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA. Alper HS; McKetta Department of Chemical Engineering, University of Texas at Austin, Austin, TX, USA. PflegerBF; Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, WI, USA. Tyo KEJ; Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA. Santos CNS; Manus Bio, Cambridge, MA 02138, USA. Ajikumar PK; Manus Bio, Cambridge, MA 02138, USA. Stephanopoulos G; Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02142, USA.
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Źródło:
Science (New York, N.Y.) [Science] 2021 Dec 24; Vol. 374 (6575), pp. 1563-1565. Date of Electronic Publication: 2021 Dec 23.
Optimization of a T7-RNA polymerase system in Synechococcus sp. PCC 7002 mirrors the protein overproduction phenotype from E. coli BL21(DE3).
Autorzy:
Jones CM; Chemical Engineering, School for Engineering Matter, Transport, and Energy, Arizona State University, Tempe, AZ, 85287, USA. Korosh TC; Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, WI, 53706, USA. Nielsen DR; Chemical Engineering, School for Engineering Matter, Transport, and Energy, Arizona State University, Tempe, AZ, 85287, USA. PflegerBF; Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, WI, 53706, USA. pfleger@engr.wisc.edu.
Model-driven analysis of mutant fitness experiments improves genome-scale metabolic models of Zymomonas mobilis ZM4.
Autorzy:
Ong WK; Department of Chemical and Biological Engineering, University of Wisconsin - Madison, Madison, Wisconsin, United States of America.; DOE Great Lakes Bioenergy Research Center, Univ. of Wisconsin-Madison - Madison, Madison, Wisconsin, United States of America. Courtney DK; Department of Chemical and Biological Engineering, University of Wisconsin - Madison, Madison, Wisconsin, United States of America.; DOE Great Lakes Bioenergy Research Center, Univ. of Wisconsin-Madison - Madison, Madison, Wisconsin, United States of America. Pan S; Department of Chemical and Biological Engineering, University of Wisconsin - Madison, Madison, Wisconsin, United States of America.; DOE Great Lakes Bioenergy Research Center, Univ. of Wisconsin-Madison - Madison, Madison, Wisconsin, United States of America. Andrade RB; Department of Chemical and Biological Engineering, University of Wisconsin - Madison, Madison, Wisconsin, United States of America. Kiley PJ; DOE Great Lakes Bioenergy Research Center, Univ. of Wisconsin-Madison - Madison, Madison, Wisconsin, United States of America.; Department of Biomolecular Chemistry, University of Wisconsin - Madison, Madison, Wisconsin, United States of America. PflegerBF; Department of Chemical and Biological Engineering, University of Wisconsin - Madison, Madison, Wisconsin, United States of America.; DOE Great Lakes Bioenergy Research Center, Univ. of Wisconsin-Madison - Madison, Madison, Wisconsin, United States of America. Reed JL; Department of Chemical and Biological Engineering, University of Wisconsin - Madison, Madison, Wisconsin, United States of America.; DOE Great Lakes Bioenergy Research Center, Univ. of Wisconsin-Madison - Madison, Madison, Wisconsin, United States of America.
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Źródło:
PLoS computational biology [PLoS Comput Biol] 2020 Aug 17; Vol. 16 (8), pp. e1008137. Date of Electronic Publication: 2020 Aug 17 (Print Publication: 2020).
Typ publikacji:
Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.
Genetic tools for reliable gene expression and recombineering in Pseudomonas putida.
Autorzy:
Cook TB; Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, USA. Rand JM; Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, USA. Nurani W; Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, USA. Courtney DK; Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, USA. Liu SA; Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, USA.; Waunakee High School, Waunakee, USA. PflegerBF; Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, USA. pfleger@engr.wisc.edu.; Microbiology Doctoral Training Program, University of Wisconsin-Madison, Madison, USA. pfleger@engr.wisc.edu.
Functional genomics analysis of free fatty acid production under continuous phosphate limiting conditions.
Autorzy:
Youngquist JT; Department of Chemical and Biological Engineering, University of Wisconsin-Madison, 3629 Engineering Hall, 1415 Engineering Drive, Madison, WI, USA. Korosh TC; Department of Chemical and Biological Engineering, University of Wisconsin-Madison, 3629 Engineering Hall, 1415 Engineering Drive, Madison, WI, USA.; Graduate Program in Environmental Chemistry and Technology, University of Wisconsin-Madison, Madison, WI, USA. PflegerBF; Department of Chemical and Biological Engineering, University of Wisconsin-Madison, 3629 Engineering Hall, 1415 Engineering Drive, Madison, WI, USA. pfleger@engr.wisc.edu.; Microbiology Doctoral Training Program, University of Wisconsin-Madison, Madison, WI, USA. pfleger@engr.wisc.edu.
Efflux systems in bacteria and their metabolic engineering applications.
Autorzy:
Jones CM; Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, WI, 53706, USA. Hernández Lozada NJ; Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, WI, 53706, USA. PflegerBF; Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, WI, 53706, USA. pfleger@engr.wisc.edu.; Microbiology Doctoral Training Program, University of Wisconsin-Madison, Madison, WI, 53706, USA. pfleger@engr.wisc.edu.; 3629 Engineering Hall, 1415 Engineering Drive, Madison, WI, 53706, USA. pfleger@engr.wisc.edu.
An organic acid based counter selection system for cyanobacteria.
Autorzy:
Begemann MB; Microbiology Doctoral Training Program, University of Wisconsin-Madison, Madison, Wisconsin, United States of America ; Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, Wisconsin, United States of America. Zess EK Walters EM Schmitt EF Markley AL PflegerBF
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Źródło:
PloS one [PLoS One] 2013 Oct 01; Vol. 8 (10), pp. e76594. Date of Electronic Publication: 2013 Oct 01 (Print Publication: 2013).
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
Free fatty acid production in Escherichia coli under phosphate-limited conditions.
Autorzy:
Youngquist JT; Department of Chemical and Biological Engineering, University of Wisconsin-Madison, 3629 Engineering Hall, 1415 Engineering Dr, Madison, WI 53706, USA. Rose JP PflegerBF
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