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Wyszukujesz frazę ""Polymerase Chain Reaction"" wg kryterium: Temat


Tytuł:
Adaptive Filtering Framework to Remove Nonspecific and Low-Efficiency Reactions in Multiplex Digital PCR Based on Sigmoidal Trends.
Autorzy:
Miglietta L; Department of Infectious Disease, Faculty of Medicine, Imperial College London, LondonW12 0NN, U.K.; Department of Electrical and Electronic Engineering, Faculty of Engineering, Imperial College London, LondonSW7 2AZ, U.K.
Xu K; Department of Infectious Disease, Faculty of Medicine, Imperial College London, LondonW12 0NN, U.K.; Department of Electrical and Electronic Engineering, Faculty of Engineering, Imperial College London, LondonSW7 2AZ, U.K.
Chhaya P; Department of Electrical and Electronic Engineering, Faculty of Engineering, Imperial College London, LondonSW7 2AZ, U.K.
Kreitmann L; Department of Infectious Disease, Faculty of Medicine, Imperial College London, LondonW12 0NN, U.K.
Hill-Cawthorne K; Department of Infectious Disease, Faculty of Medicine, Imperial College London, LondonW12 0NN, U.K.
Bolt F; Department of Infectious Disease, Faculty of Medicine, Imperial College London, LondonW12 0NN, U.K.
Holmes A; Department of Infectious Disease, Faculty of Medicine, Imperial College London, LondonW12 0NN, U.K.
Georgiou P; Department of Electrical and Electronic Engineering, Faculty of Engineering, Imperial College London, LondonSW7 2AZ, U.K.
Rodriguez-Manzano J; Department of Infectious Disease, Faculty of Medicine, Imperial College London, LondonW12 0NN, U.K.
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Źródło:
Analytical chemistry [Anal Chem] 2022 Oct 18; Vol. 94 (41), pp. 14159-14168. Date of Electronic Publication: 2022 Oct 03.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
Algorithms*
Multiplex Polymerase Chain Reaction*
Kinetics ; Real-Time Polymerase Chain Reaction
Czasopismo naukowe
Tytuł:
Designing highly multiplex PCR primer sets with Simulated Annealing Design using Dimer Likelihood Estimation (SADDLE).
Autorzy:
Xie NG; Department of Bioengineering, Rice University, Houston, TX, USA.
Wang MX; Department of Bioengineering, Rice University, Houston, TX, USA.
Song P; Department of Bioengineering, Rice University, Houston, TX, USA.
Mao S; Department of Medical Oncology, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China.
Wang Y; NuProbe China, Shanghai, China.
Yang Y; NuProbe China, Shanghai, China.
Luo J; NuProbe China, Shanghai, China.
Ren S; Department of Medical Oncology, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China. harry_.
Zhang DY; NuProbe USA, Houston, TX, USA. .
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Źródło:
Nature communications [Nat Commun] 2022 Apr 11; Vol. 13 (1), pp. 1881. Date of Electronic Publication: 2022 Apr 11.
Typ publikacji:
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
MeSH Terms:
Algorithms*
Multiplex Polymerase Chain Reaction*/methods
DNA Primers/genetics ; Likelihood Functions ; Real-Time Polymerase Chain Reaction/methods
Czasopismo naukowe
Tytuł:
Comparison of FACS and PCR for Detection of BCMA-CAR-T Cells.
Autorzy:
Reichman A; Department of Hematology, Oncology and Rheumatology, Heidelberg University Hospital, Im Neuenheimer Feld 410, 69120 Heidelberg, Germany.
Kunz A; Department of Hematology, Oncology and Rheumatology, Heidelberg University Hospital, Im Neuenheimer Feld 410, 69120 Heidelberg, Germany.
Joedicke JJ; Department of Microenvironmental Regulation in Autoimmunity and Cancer, Max-Delbrück Center for Molecular Medicine (MDC), Robert-Rössle-Str. 10, 13125 Berlin-Buch, Germany.
Höpken UE; Department of Translational Tumor Immunology, Max-Delbrück Center for Molecular Medicine (MDC), Robert-Rössle-Str. 10, 13125 Berlin-Buch, Germany.
Keib A; Department of Hematology, Oncology and Rheumatology, Heidelberg University Hospital, Im Neuenheimer Feld 410, 69120 Heidelberg, Germany.
Neuber B; Department of Hematology, Oncology and Rheumatology, Heidelberg University Hospital, Im Neuenheimer Feld 410, 69120 Heidelberg, Germany.
Sedloev D; Department of Hematology, Oncology and Rheumatology, Heidelberg University Hospital, Im Neuenheimer Feld 410, 69120 Heidelberg, Germany.
Wang L; Department of Hematology, Oncology and Rheumatology, Heidelberg University Hospital, Im Neuenheimer Feld 410, 69120 Heidelberg, Germany.
Jiang G; Department of Hematology, Oncology and Rheumatology, Heidelberg University Hospital, Im Neuenheimer Feld 410, 69120 Heidelberg, Germany.
Hückelhoven-Krauss A; Department of Hematology, Oncology and Rheumatology, Heidelberg University Hospital, Im Neuenheimer Feld 410, 69120 Heidelberg, Germany.
Eberhardt F; Department of Hematology, Oncology and Rheumatology, Heidelberg University Hospital, Im Neuenheimer Feld 410, 69120 Heidelberg, Germany.
Müller-Tidow C; Department of Hematology, Oncology and Rheumatology, Heidelberg University Hospital, Im Neuenheimer Feld 410, 69120 Heidelberg, Germany.
Wermke M; NCT/UCC Early Clinical Trial Unit (ECTU), Medical Faculty C.-G. Carus, Technical University Dresden, Fetscherstraße 74, 01307 Dresden, Germany.
Rehm A; Department of Microenvironmental Regulation in Autoimmunity and Cancer, Max-Delbrück Center for Molecular Medicine (MDC), Robert-Rössle-Str. 10, 13125 Berlin-Buch, Germany.
Schmitt M; Department of Hematology, Oncology and Rheumatology, Heidelberg University Hospital, Im Neuenheimer Feld 410, 69120 Heidelberg, Germany.
Schmitt A; Department of Hematology, Oncology and Rheumatology, Heidelberg University Hospital, Im Neuenheimer Feld 410, 69120 Heidelberg, Germany.
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Źródło:
International journal of molecular sciences [Int J Mol Sci] 2022 Jan 14; Vol. 23 (2). Date of Electronic Publication: 2022 Jan 14.
Typ publikacji:
Journal Article
MeSH Terms:
Flow Cytometry*/methods
Flow Cytometry*/standards
Polymerase Chain Reaction*/methods
Polymerase Chain Reaction*/standards
B-Cell Maturation Antigen/*metabolism
Receptors, Chimeric Antigen/*metabolism
T-Lymphocytes/*metabolism
B-Cell Maturation Antigen/genetics ; Biomarkers ; Humans ; Immunophenotyping ; Immunotherapy, Adoptive/methods ; Immunotherapy, Adoptive/standards ; Real-Time Polymerase Chain Reaction ; Receptors, Chimeric Antigen/genetics ; Reproducibility of Results ; Sensitivity and Specificity ; T-Lymphocytes/immunology
Czasopismo naukowe
Tytuł:
Evaluation of Sensitivity and Cost-Effectiveness of Molecular Methods for the Co-detection of Waterborne Pathogens in India.
Autorzy:
M A; Department of Life Sciences, University of Calicut, Malappuram, Kerala-673635, India.
Sebastian D; Department of Life Sciences, University of Calicut, Malappuram, Kerala-673635, India. .
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Źródło:
Marine biotechnology (New York, N.Y.) [Mar Biotechnol (NY)] 2021 Dec; Vol. 23 (6), pp. 955-963. Date of Electronic Publication: 2021 Oct 29.
Typ publikacji:
Journal Article
MeSH Terms:
Escherichia coli*/genetics
Multiplex Polymerase Chain Reaction*
Cost-Benefit Analysis ; DNA Primers/genetics ; Real-Time Polymerase Chain Reaction/methods
Czasopismo naukowe
Tytuł:
Specific allelic discrimination of N501Y and other SARS-CoV-2 mutations by ddPCR detects B.1.1.7 lineage in Washington State.
Autorzy:
Perchetti GA; Department of Laboratory Medicine and Pathology, Virology Division, University of Washington School of Medicine, Seattle, Washington, USA.
Zhu H; Department of Laboratory Medicine and Pathology, Virology Division, University of Washington School of Medicine, Seattle, Washington, USA.
Mills MG; Department of Laboratory Medicine and Pathology, Virology Division, University of Washington School of Medicine, Seattle, Washington, USA.
Shrestha L; Department of Laboratory Medicine and Pathology, Virology Division, University of Washington School of Medicine, Seattle, Washington, USA.
Wagner C; Department of Genome Sciences, University of Washington, Seattle, Washington, USA.; Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.
Bakhash SM; Department of Laboratory Medicine and Pathology, Virology Division, University of Washington School of Medicine, Seattle, Washington, USA.
Lin MJ; Department of Laboratory Medicine and Pathology, Virology Division, University of Washington School of Medicine, Seattle, Washington, USA.
Xie H; Department of Laboratory Medicine and Pathology, Virology Division, University of Washington School of Medicine, Seattle, Washington, USA.
Huang ML; Department of Laboratory Medicine and Pathology, Virology Division, University of Washington School of Medicine, Seattle, Washington, USA.
Mathias P; Department of Laboratory Medicine and Pathology, Virology Division, University of Washington School of Medicine, Seattle, Washington, USA.; Department of Biomedical Informatics and Medical Education, University of Washington School of Medicine, Seattle, Washington, USA.
Bedford T; Department of Genome Sciences, University of Washington, Seattle, Washington, USA.; Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.
Jerome KR; Department of Laboratory Medicine and Pathology, Virology Division, University of Washington School of Medicine, Seattle, Washington, USA.; Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.
Greninger AL; Department of Laboratory Medicine and Pathology, Virology Division, University of Washington School of Medicine, Seattle, Washington, USA.; Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.
Roychoudhury P; Department of Laboratory Medicine and Pathology, Virology Division, University of Washington School of Medicine, Seattle, Washington, USA.; Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.
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Źródło:
Journal of medical virology [J Med Virol] 2021 Oct; Vol. 93 (10), pp. 5931-5941. Date of Electronic Publication: 2021 Jul 03.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
COVID-19 Nucleic Acid Testing*
Real-Time Polymerase Chain Reaction*
SARS-CoV-2/*isolation & purification
Alleles ; COVID-19/diagnosis ; COVID-19/epidemiology ; Genotype ; High-Throughput Nucleotide Sequencing ; Humans ; Mutation ; Phylogeny ; Reverse Transcriptase Polymerase Chain Reaction ; SARS-CoV-2/genetics ; Spike Glycoprotein, Coronavirus/genetics ; Time Factors ; Washington/epidemiology
Czasopismo naukowe
Tytuł:
Development of a dual-labeled, hydrolysis probe-based, real-time quantitative PCR assay for detection of both genotypes of duck circovirus-1 (DuCV-1) and DuCV-2.
Autorzy:
Zhang L; Shandong Agricultural University, Tai'an, Shandong, China.
Jiang W; China Animal Health and Epidemiology Center, Qingdao, Shandong, China.
Zhang F; Shandong Agricultural University, Tai'an, Shandong, China.
Li Y; China Animal Health and Epidemiology Center, Qingdao, Shandong, China.
Li J; China Animal Health and Epidemiology Center, Qingdao, Shandong, China.
Liang S; Inner Mongolia Agricultural University, Huhhot, Inner Mongolia, China.
Yu X; China Animal Health and Epidemiology Center, Qingdao, Shandong, China.
Peng C; China Animal Health and Epidemiology Center, Qingdao, Shandong, China.
Liu S; China Animal Health and Epidemiology Center, Qingdao, Shandong, China.
Wang J; China Animal Health and Epidemiology Center, Qingdao, Shandong, China.
Sun S; Shandong Agricultural University, Tai'an, Shandong, China. .
Liu H; China Animal Health and Epidemiology Center, Qingdao, Shandong, China. .
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Źródło:
Virus genes [Virus Genes] 2021 Oct; Vol. 57 (5), pp. 453-458. Date of Electronic Publication: 2021 Jul 26.
Typ publikacji:
Journal Article
MeSH Terms:
Polymerase Chain Reaction*
Circoviridae Infections/*diagnosis
Circovirus/*isolation & purification
Poultry Diseases/*diagnosis
Animals ; Circoviridae Infections/genetics ; Circoviridae Infections/virology ; Circovirus/genetics ; Circovirus/pathogenicity ; DNA, Viral/genetics ; Genotype ; Hydrolysis ; Poultry Diseases/genetics ; Poultry Diseases/virology ; Real-Time Polymerase Chain Reaction
SCR Organism:
Duck circovirus
Czasopismo naukowe
Tytuł:
Processing Hundreds of SARS-CoV-2 Samples with an In-House PCR-Based Method without Robotics.
Autorzy:
Mair T; Institute of Biophysics, Johannes Kepler University, 4020 Linz, Austria.
Ivankovic M; Institute of Biophysics, Johannes Kepler University, 4020 Linz, Austria.
Paar C; Institute of Laboratory Medicine, Johannes Kepler Universitätsklinikum, 4020 Linz, Austria.
Salzer HJF; Department of Pulmonology, Johannes Kepler Universitätsklinikum, 4020 Linz, Austria.
Heissl A; Department of Biosciences, University of Salzburg, 5020 Salzburg, Austria.
Lamprecht B; Department of Pulmonology, Johannes Kepler Universitätsklinikum, 4020 Linz, Austria.
Schreier-Lechner E; Institute of Laboratory Medicine, Johannes Kepler Universitätsklinikum, 4020 Linz, Austria.
Tiemann-Boege I; Institute of Biophysics, Johannes Kepler University, 4020 Linz, Austria.
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Źródło:
Viruses [Viruses] 2021 Aug 28; Vol. 13 (9). Date of Electronic Publication: 2021 Aug 28.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
Polymerase Chain Reaction*/methods
Polymerase Chain Reaction*/standards
SARS-CoV-2*/genetics
COVID-19/*diagnosis
COVID-19/*virology
COVID-19 Nucleic Acid Testing/*methods
COVID-19 Nucleic Acid Testing/standards ; Humans ; Reagent Kits, Diagnostic ; Real-Time Polymerase Chain Reaction/methods ; Real-Time Polymerase Chain Reaction/standards ; Reproducibility of Results ; Sensitivity and Specificity ; Viral Load
Czasopismo naukowe
Tytuł:
DNA barcode, multiplex PCR and qPCR assay for diagnosis of pathogens infecting pulse crops to facilitate safe exchange and healthy conservation of germplasm.
Autorzy:
Tripathi A; Division of Plant Quarantine, ICAR-National Bureau of Plant Genetic Resources, New Delhi, 110 012, India.
Rai A; Division of Plant Quarantine, ICAR-National Bureau of Plant Genetic Resources, New Delhi, 110 012, India.
Dubey SC; Division of Plant Quarantine, ICAR-National Bureau of Plant Genetic Resources, New Delhi, 110 012, India. .
Akhtar J; Division of Plant Quarantine, ICAR-National Bureau of Plant Genetic Resources, New Delhi, 110 012, India.
Kumar P; Division of Plant Quarantine, ICAR-National Bureau of Plant Genetic Resources, New Delhi, 110 012, India.
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Źródło:
Archives of microbiology [Arch Microbiol] 2021 Jul; Vol. 203 (5), pp. 2575-2589. Date of Electronic Publication: 2021 Mar 08.
Typ publikacji:
Journal Article
MeSH Terms:
DNA Barcoding, Taxonomic*
Polymerase Chain Reaction*
Crops, Agricultural/*microbiology
Fabaceae/*microbiology
Fungi/*classification
Plant Diseases/*microbiology
Alternaria/classification ; Alternaria/genetics ; Alternaria/isolation & purification ; Ascomycota/classification ; Ascomycota/genetics ; Ascomycota/isolation & purification ; DNA, Fungal/genetics ; Fungi/genetics ; Fungi/isolation & purification ; Fusarium/classification ; Fusarium/genetics ; Fusarium/isolation & purification ; Multiplex Polymerase Chain Reaction ; Phylogeny ; Real-Time Polymerase Chain Reaction ; Rhizoctonia/classification ; Rhizoctonia/genetics ; Rhizoctonia/isolation & purification
SCR Organism:
Alternaria alternata; Ascochyta rabiei; Fusarium oxysporum; Macrophomina phaseolina; Pseudocercospora nelumbonicola; Rhizoctonia solani; Sclerotinia sclerotiorum
Czasopismo naukowe
Tytuł:
Rapid preparation of gastrointestinal nematode eggs from faeces for PCR identification.
Autorzy:
Hedley N; Department of Chemistry and Biotechnology, Swinburne University of Technology, John Street, Hawthorn, VIC 3122, Australia. Electronic address: .
Richards DG; Gretals Australia, Unit 8/1140 Nepean Highway, Mornington, VIC 3931, Australia.
Palombo EA; Department of Chemistry and Biotechnology, Swinburne University of Technology, John Street, Hawthorn, VIC 3122, Australia.
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Źródło:
Journal of microbiological methods [J Microbiol Methods] 2021 Aug; Vol. 187, pp. 106257. Date of Electronic Publication: 2021 May 29.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
Polymerase Chain Reaction*
DNA, Helminth/*isolation & purification
Feces/*parasitology
Nematoda/*classification
Nematode Infections/*veterinary
Parasitology/*methods
Animals ; DNA Primers ; Gastrointestinal Tract/parasitology ; Multiplex Polymerase Chain Reaction ; Nematoda/genetics ; Nematoda/isolation & purification ; Nematode Infections/parasitology ; Ovum ; Sheep ; Sheep Diseases/parasitology
Czasopismo naukowe
Tytuł:
Identification of the SARS-CoV-2 Delta variant C22995A using a high-resolution melting curve RT-FRET-PCR.
Autorzy:
Barua S; College of Veterinary Medicine, Auburn University, Auburn, AL, USA.
Bai J; Kansas State Veterinary Diagnostic Laboratory, College of Veterinary Medicine, Kansas State University, Manhattan, KS, USA.
Kelly PJ; Department of Veterinary Clinical Sciences, Ross University School of Veterinary Medicine, St. Kitts & Nevis.
Hanzlicek G; Kansas State Veterinary Diagnostic Laboratory, College of Veterinary Medicine, Kansas State University, Manhattan, KS, USA.
Noll L; Kansas State Veterinary Diagnostic Laboratory, College of Veterinary Medicine, Kansas State University, Manhattan, KS, USA.
Johnson C; College of Veterinary Medicine, Auburn University, Auburn, AL, USA.
Yin JH; College of Veterinary Medicine, Auburn University, Auburn, AL, USA.
Wang C; College of Veterinary Medicine, Auburn University, Auburn, AL, USA.
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Źródło:
Emerging microbes & infections [Emerg Microbes Infect] 2022 Dec; Vol. 11 (1), pp. 14-17.
Typ publikacji:
Letter
MeSH Terms:
Fluorescence Resonance Energy Transfer*/methods
Reverse Transcriptase Polymerase Chain Reaction*/methods
COVID-19/*virology
SARS-CoV-2/*genetics
Alleles ; Amino Acid Substitution ; Humans ; Mutation ; RNA, Viral ; SARS-CoV-2/classification ; Spike Glycoprotein, Coronavirus/genetics
SCR Organism:
SARS-CoV-2 variants
Opinia redakcyjna
Tytuł:
Heat-Treated Meat Origin Tracing and Authenticity through a Practical Multiplex Polymerase Chain Reaction Approach.
Autorzy:
Cheng Y; Key Laboratory of Animal Protein Deep Processing Technology of Zhejiang Province, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315800, China.
Wang S; Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Province, Huzhou University, Huzhou 313000, China.
Ju S; Key Laboratory of Animal Protein Deep Processing Technology of Zhejiang Province, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315800, China.
Zhou S; Key Laboratory of Animal Protein Deep Processing Technology of Zhejiang Province, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315800, China.
Zeng X; Key Laboratory of Animal Protein Deep Processing Technology of Zhejiang Province, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315800, China.
Wu Z; Key Laboratory of Animal Protein Deep Processing Technology of Zhejiang Province, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315800, China.
Pan D; Key Laboratory of Animal Protein Deep Processing Technology of Zhejiang Province, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315800, China.
Zhong G; Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing 211166, China.
Cai Z; Key Laboratory of Animal Protein Deep Processing Technology of Zhejiang Province, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315800, China.
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Źródło:
Nutrients [Nutrients] 2022 Nov 09; Vol. 14 (22). Date of Electronic Publication: 2022 Nov 09.
Typ publikacji:
Journal Article
MeSH Terms:
Multiplex Polymerase Chain Reaction*/methods
Hot Temperature*
Cattle ; Swine ; Sheep ; Dogs ; Humans ; Animals ; Food Contamination/analysis ; Meat/analysis ; DNA/analysis
Czasopismo naukowe
Tytuł:
Diagnosis of an Acinetobacter pittii from a patient in China with a multiplex PCR-based targeted gene sequencing platform of the cerebrospinal fluid: A case report with literature review.
Autorzy:
Feng W; Department of Critical Care Medicine, Fengtai Youanmen Hospital, Beijing, China.
Jiang X; Intensive Care Unit, Renhe Hospital, Beijing, China.
Zhang R; Department of Neurology, Yunnan St John's Hospital, Yunnan, China.
Guo Z; Department of Science and Technology, Shanghai, China.
Gao D; Department of Neurology, Xuanwu Hospital of Capital Medical University, Beijing, China.
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Źródło:
Medicine [Medicine (Baltimore)] 2022 Oct 21; Vol. 101 (42), pp. e31130.
Typ publikacji:
Review; Case Reports; Journal Article
MeSH Terms:
Multiplex Polymerase Chain Reaction*
Ceftazidime*
Humans ; Male ; Middle Aged ; Vancomycin ; Xylose ; Valproic Acid ; High-Throughput Nucleotide Sequencing/methods ; Staphylococcus epidermidis ; Mannitol
SCR Organism:
Acinetobacter pittii
Czasopismo naukowe
Tytuł:
Multiplex PCR: Aid to more-timely and directed therapeutic intervention for patients with infectious gastroenteritis.
Autorzy:
Montasser K; Clinical Pathology Department, Faculty of Medicine, Helwan University, Helwan, Egypt.
Osman HA; Tropical Medicine and Gastroenterology Department, Faculty of Medicine, South Valley University, Qena, Egypt.
Abozaid H; Clinical Pathology Department, Faculty of Medicine, Helwan University, Helwan, Egypt.
Khalil HS; Medical Microbiology and Immunology Department, Faculty of Medicine, Helwan University, Helwan, Egypt.
Hatem Amer W; Microbiology and Immunology Department, Faculty of Medicine, Tanta University, Tanta, Egypt.
Sabry AMM; Internal Medicine and Gastroenterology Department, Faculty of Medicine, Helwan University, Helwan, Egypt.
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Źródło:
Medicine [Medicine (Baltimore)] 2022 Oct 14; Vol. 101 (41), pp. e31022.
Typ publikacji:
Journal Article; Randomized Controlled Trial
MeSH Terms:
Gastroenteritis*/diagnosis
Gastroenteritis*/microbiology
Multiplex Polymerase Chain Reaction*/methods
Diarrhea/diagnosis ; Feces/microbiology ; Humans ; Molecular Diagnostic Techniques/methods ; Prospective Studies ; Sensitivity and Specificity
Czasopismo naukowe
Tytuł:
Developing a multiplex PCR-based assay kit for bloodstream infection by analyzing genomic big data.
Autorzy:
Zhang D; Department of Infectious Diseases, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China.; Ningbo Health Gene Technologies Co., Ltd., Ningbo, China.
Luo Y; Ningbo Health Gene Technologies Co., Ltd., Ningbo, China.
Zeng X; Ningbo Health Gene Technologies Co., Ltd., Ningbo, China.
Yu Y; Department of Infectious Diseases, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China.; Key Laboratory of Microbial Technology and Bioinformatics of Zhejiang Province, Hangzhou, China.; Regional Medical Center for National Institute of Respiratory Diseases, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China.
Wu Y; Ningbo Health Gene Technologies Co., Ltd., Ningbo, China.
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Źródło:
Journal of clinical laboratory analysis [J Clin Lab Anal] 2022 Oct; Vol. 36 (10), pp. e24686. Date of Electronic Publication: 2022 Aug 31.
Typ publikacji:
Journal Article
MeSH Terms:
Multiplex Polymerase Chain Reaction*/methods
Sepsis*/diagnosis
Sepsis*/genetics
Sepsis*/microbiology
Anti-Bacterial Agents ; Bacteria/genetics ; Big Data ; Genomics ; Hormones ; Humans ; Immunosuppressive Agents ; Sensitivity and Specificity
Czasopismo naukowe
Tytuł:
Evaluation of a novel multiplex qPCR method for rapid detection and quantification of pathogens associated with calf diarrhoea.
Autorzy:
Pansri P; DNA Diagnostic A/S, Risskov, Denmark.
Svensmark B; LVK Veterinary Cattle Practice, Hobro, Denmark.
Liu G; Department of Veterinary and Animal Sciences, University of Copenhagen, Copenhagen, Denmark.
Thamsborg SM; Department of Veterinary and Animal Sciences, University of Copenhagen, Copenhagen, Denmark.
Kudirkiene E; Department of Veterinary and Animal Sciences, University of Copenhagen, Copenhagen, Denmark.
Nielsen HV; Department of Microbiology and Infection Control, Statens Serum Institut, Copenhagen, Denmark.
Goecke NB; Department of Veterinary and Animal Sciences, University of Copenhagen, Copenhagen, Denmark.
Olsen JE; Department of Veterinary and Animal Sciences, University of Copenhagen, Copenhagen, Denmark.
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Źródło:
Journal of applied microbiology [J Appl Microbiol] 2022 Oct; Vol. 133 (4), pp. 2516-2527. Date of Electronic Publication: 2022 Jul 31.
Typ publikacji:
Journal Article
MeSH Terms:
Multiplex Polymerase Chain Reaction*/methods
Nucleic Acids*
Animals ; Bacteria/genetics ; Cattle ; Diarrhea/diagnosis ; Diarrhea/microbiology ; Diarrhea/veterinary ; Feces/microbiology ; Sensitivity and Specificity
Czasopismo naukowe
Tytuł:
Rapid and sensitive identification of pleural and peritoneal infections by droplet digital PCR.
Autorzy:
Zhou F; College of Medical Technology, Zhejiang Chinese Medical University, Hangzhou, 310053, Zhejiang, People's Republic of China.
Sun S; Department of Clinical Laboratory, The First Affiliated Hospital of Zhejiang Chinese Medical University, 54 Youdian Road, Hangzhou, 310006, Zhejiang, People's Republic of China.; The First Clinical Medical College of Zhejiang Chinese Medical University, Hangzhou, 310053, Zhejiang, People's Republic of China.
Sun X; Department of Clinical Laboratory, The First Affiliated Hospital of Zhejiang Chinese Medical University, 54 Youdian Road, Hangzhou, 310006, Zhejiang, People's Republic of China.
Chen Y; Department of Clinical Laboratory, The First Affiliated Hospital of Zhejiang Chinese Medical University, 54 Youdian Road, Hangzhou, 310006, Zhejiang, People's Republic of China.; The First Clinical Medical College of Zhejiang Chinese Medical University, Hangzhou, 310053, Zhejiang, People's Republic of China.
Yang X; Department of Clinical Laboratory, The First Affiliated Hospital of Zhejiang Chinese Medical University, 54 Youdian Road, Hangzhou, 310006, Zhejiang, People's Republic of China. .
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Źródło:
Folia microbiologica [Folia Microbiol (Praha)] 2021 Apr; Vol. 66 (2), pp. 213-219. Date of Electronic Publication: 2020 Nov 07.
Typ publikacji:
Journal Article
MeSH Terms:
Real-Time Polymerase Chain Reaction*
Humans ; Polymerase Chain Reaction ; Sensitivity and Specificity
Czasopismo naukowe
Tytuł:
Smaller reaction volume of triplex taqman real-time reverse transcription-PCR assays for diagnosing coronavirus disease 2019.
Autorzy:
Dong W; Key Laboratory for Molecular Genetic Mechanisms and Intervention Research on High Altitude Disease of Tibet Autonomous Region, School of Medicine, Xizang Minzu University, Xianyang, China.
Yang X; Key Laboratory for Molecular Genetic Mechanisms and Intervention Research on High Altitude Disease of Tibet Autonomous Region, School of Medicine, Xizang Minzu University, Xianyang, China.
Li J; Key Laboratory for Molecular Genetic Mechanisms and Intervention Research on High Altitude Disease of Tibet Autonomous Region, School of Medicine, Xizang Minzu University, Xianyang, China.
Zhang Z; Key Laboratory for Molecular Genetic Mechanisms and Intervention Research on High Altitude Disease of Tibet Autonomous Region, School of Medicine, Xizang Minzu University, Xianyang, China.
Liu L; Key Laboratory for Molecular Genetic Mechanisms and Intervention Research on High Altitude Disease of Tibet Autonomous Region, School of Medicine, Xizang Minzu University, Xianyang, China.
Zhao Z; Key Laboratory for Molecular Genetic Mechanisms and Intervention Research on High Altitude Disease of Tibet Autonomous Region, School of Medicine, Xizang Minzu University, Xianyang, China.; Department of Basic Medical Sciences, Taizhou University, Taizhou, China.
Kang L; Key Laboratory for Molecular Genetic Mechanisms and Intervention Research on High Altitude Disease of Tibet Autonomous Region, School of Medicine, Xizang Minzu University, Xianyang, China.
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Źródło:
Journal of clinical laboratory analysis [J Clin Lab Anal] 2022 Jan; Vol. 36 (1), pp. e24137. Date of Electronic Publication: 2021 Dec 03.
Typ publikacji:
Journal Article
MeSH Terms:
COVID-19 Nucleic Acid Testing/*methods
Real-Time Polymerase Chain Reaction/*methods
SARS-CoV-2/*genetics
DNA Primers ; Humans ; RNA, Viral ; Reproducibility of Results ; Reverse Transcriptase Polymerase Chain Reaction/methods ; Sensitivity and Specificity
Czasopismo naukowe
Tytuł:
Novel dual multiplex real-time RT-PCR assays for the rapid detection of SARS-CoV-2, influenza A/B, and respiratory syncytial virus using the BD MAX open system.
Autorzy:
Chung HY; Division of Clinical Pathology, Department of Pathology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, ROC.
Jian MJ; Division of Clinical Pathology, Department of Pathology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, ROC.
Chang CK; Division of Clinical Pathology, Department of Pathology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, ROC.
Lin JC; Division of Infectious Diseases and Tropical Medicine, Department of Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, ROC.
Yeh KM; Division of Infectious Diseases and Tropical Medicine, Department of Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, ROC.
Chen CW; Division of Pulmonary and Critical Care Medicine, Department of Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, ROC.
Chiu SK; Division of Infectious Diseases and Tropical Medicine, Department of Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, ROC.
Wang YH; Division of Clinical Pathology, Department of Pathology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, ROC.
Liao SJ; Division of Clinical Pathology, Department of Pathology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, ROC.
Li SY; Division of Clinical Pathology, Department of Pathology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, ROC.
Hsieh SS; Division of Clinical Pathology, Department of Pathology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, ROC.
Tsai SH; Department of Emergency Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, ROC.
Perng CL; Division of Clinical Pathology, Department of Pathology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, ROC.
Yang JR; Centers for Disease Control, Taipei, Taiwan, ROC.
Liu MT; Centers for Disease Control, Taipei, Taiwan, ROC.
Chang FY; Division of Infectious Diseases and Tropical Medicine, Department of Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, ROC.
Shang HS; Division of Clinical Pathology, Department of Pathology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, ROC.
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Źródło:
Emerging microbes & infections [Emerg Microbes Infect] 2021 Dec; Vol. 10 (1), pp. 161-166.
Typ publikacji:
Journal Article
MeSH Terms:
COVID-19/*diagnosis
Influenza, Human/*diagnosis
Polymerase Chain Reaction/*methods
Respiratory Syncytial Virus Infections/*diagnosis
SARS-CoV-2/*genetics
Adolescent ; Adult ; Aged ; Aged, 80 and over ; COVID-19 Testing ; Coinfection/epidemiology ; Female ; Humans ; Male ; Middle Aged ; Multiplex Polymerase Chain Reaction/methods ; Real-Time Polymerase Chain Reaction/methods ; Reverse Transcriptase Polymerase Chain Reaction/methods ; Young Adult
Czasopismo naukowe
Tytuł:
End-point RT-PCR: A potential alternative for diagnosing coronavirus disease 2019 (COVID-19).
Autorzy:
Silva Júnior JVJ; Setor de Virologia, Laboratório de Imunopatologia Keizo Asami, Universidade Federal de Pernambuco, Pernambuco, Brazil; Departamento de Microbiologia e Parasitologia, Universidade Federal de Santa Maria, Rio Grande do Sul, Brazil; Setor de Virologia, Departamento de Medicina Veterinária Preventiva, Universidade Federal de Santa Maria, Rio Grande do Sul, Brazil.
Merchioratto I; Setor de Virologia, Departamento de Medicina Veterinária Preventiva, Universidade Federal de Santa Maria, Rio Grande do Sul, Brazil.
de Oliveira PSB; Setor de Virologia, Departamento de Medicina Veterinária Preventiva, Universidade Federal de Santa Maria, Rio Grande do Sul, Brazil.
Rocha Lopes TR; Setor de Virologia, Laboratório de Imunopatologia Keizo Asami, Universidade Federal de Pernambuco, Pernambuco, Brazil; Setor de Virologia, Departamento de Medicina Veterinária Preventiva, Universidade Federal de Santa Maria, Rio Grande do Sul, Brazil.
Brites PC; Laboratório de Análises Clínicas, Setor de Biologia Molecular, Hospital Universitário de Santa Maria, Rio Grande do Sul, Brazil.
de Oliveira EM; Laboratório de Análises Clínicas, Setor de Biologia Molecular, Hospital Universitário de Santa Maria, Rio Grande do Sul, Brazil.
Weiblen R; Setor de Virologia, Departamento de Medicina Veterinária Preventiva, Universidade Federal de Santa Maria, Rio Grande do Sul, Brazil.
Flores EF; Setor de Virologia, Departamento de Medicina Veterinária Preventiva, Universidade Federal de Santa Maria, Rio Grande do Sul, Brazil. Electronic address: .
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Źródło:
Journal of virological methods [J Virol Methods] 2021 Feb; Vol. 288, pp. 114007. Date of Electronic Publication: 2020 Oct 29.
Typ publikacji:
Journal Article
MeSH Terms:
Reverse Transcriptase Polymerase Chain Reaction*
COVID-19/*diagnosis
COVID-19/*virology
COVID-19 Nucleic Acid Testing/*methods
SARS-CoV-2/*genetics
Humans ; RNA, Viral ; Real-Time Polymerase Chain Reaction ; Sensitivity and Specificity
Czasopismo naukowe
Tytuł:
Assessing a chip based rapid RTPCR test for SARS CoV-2 detection (TrueNat assay): A diagnostic accuracy study.
Autorzy:
Ghoshal U; Department of Microbiology, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow, India.
Garg A; Department of Microbiology, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow, India.
Vasanth S; Department of Microbiology, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow, India.
Arya AK; Department of Microbiology, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow, India.
Pandey A; Department of Microbiology, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow, India.
Tejan N; Department of Microbiology, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow, India.
Patel V; Department of Microbiology, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow, India.
Singh VP; Department of Microbiology, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow, India.
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Źródło:
PloS one [PLoS One] 2021 Oct 13; Vol. 16 (10), pp. e0257834. Date of Electronic Publication: 2021 Oct 13 (Print Publication: 2021).
Typ publikacji:
Journal Article
MeSH Terms:
COVID-19/*diagnosis
Reverse Transcriptase Polymerase Chain Reaction/*methods
SARS-CoV-2/*genetics
COVID-19/virology ; COVID-19 Testing ; Humans ; Point-of-Care Systems ; Real-Time Polymerase Chain Reaction/instrumentation ; Retrospective Studies ; Reverse Transcriptase Polymerase Chain Reaction/instrumentation ; SARS-CoV-2/isolation & purification ; Sensitivity and Specificity
Czasopismo naukowe

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