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Tytuł :
End-point RT-PCR: A potential alternative for diagnosing coronavirus disease 2019 (COVID-19).
Autorzy :
Silva Júnior JVJ; Setor de Virologia, Laboratório de Imunopatologia Keizo Asami, Universidade Federal de Pernambuco, Pernambuco, Brazil; Departamento de Microbiologia e Parasitologia, Universidade Federal de Santa Maria, Rio Grande do Sul, Brazil; Setor de Virologia, Departamento de Medicina Veterinária Preventiva, Universidade Federal de Santa Maria, Rio Grande do Sul, Brazil.
Merchioratto I; Setor de Virologia, Departamento de Medicina Veterinária Preventiva, Universidade Federal de Santa Maria, Rio Grande do Sul, Brazil.
de Oliveira PSB; Setor de Virologia, Departamento de Medicina Veterinária Preventiva, Universidade Federal de Santa Maria, Rio Grande do Sul, Brazil.
Rocha Lopes TR; Setor de Virologia, Laboratório de Imunopatologia Keizo Asami, Universidade Federal de Pernambuco, Pernambuco, Brazil; Setor de Virologia, Departamento de Medicina Veterinária Preventiva, Universidade Federal de Santa Maria, Rio Grande do Sul, Brazil.
Brites PC; Laboratório de Análises Clínicas, Setor de Biologia Molecular, Hospital Universitário de Santa Maria, Rio Grande do Sul, Brazil.
de Oliveira EM; Laboratório de Análises Clínicas, Setor de Biologia Molecular, Hospital Universitário de Santa Maria, Rio Grande do Sul, Brazil.
Weiblen R; Setor de Virologia, Departamento de Medicina Veterinária Preventiva, Universidade Federal de Santa Maria, Rio Grande do Sul, Brazil.
Flores EF; Setor de Virologia, Departamento de Medicina Veterinária Preventiva, Universidade Federal de Santa Maria, Rio Grande do Sul, Brazil. Electronic address: .
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Źródło :
Journal of virological methods [J Virol Methods] 2021 Feb; Vol. 288, pp. 114007. Date of Electronic Publication: 2020 Oct 29.
Typ publikacji :
Journal Article
MeSH Terms :
Reverse Transcriptase Polymerase Chain Reaction*
COVID-19/*diagnosis
COVID-19/*virology
COVID-19 Nucleic Acid Testing/*methods
SARS-CoV-2/*genetics
Humans ; RNA, Viral ; Real-Time Polymerase Chain Reaction ; Sensitivity and Specificity
Czasopismo naukowe
Tytuł :
Highly sensitive detection of SARS-CoV-2 RNA by multiplex rRT-PCR for molecular diagnosis of COVID-19 by clinical laboratories.
Autorzy :
Ishige T; Division of Laboratory Medicine, Chiba University Hospital, 1-8-1 Inohana, Chuo-ward, Chiba-city, Chiba 266-8677, Japan. Electronic address: .
Murata S; Division of Laboratory Medicine, Chiba University Hospital, 1-8-1 Inohana, Chuo-ward, Chiba-city, Chiba 266-8677, Japan.
Taniguchi T; Department of Infectious Diseases, Chiba University Hospital, 1-8-1 Inohana, Chuo-ward, Chiba-city, Chiba 266-8677, Japan.
Miyabe A; Division of Laboratory Medicine, Chiba University Hospital, 1-8-1 Inohana, Chuo-ward, Chiba-city, Chiba 266-8677, Japan.
Kitamura K; Division of Laboratory Medicine, Chiba University Hospital, 1-8-1 Inohana, Chuo-ward, Chiba-city, Chiba 266-8677, Japan.
Kawasaki K; Division of Laboratory Medicine, Chiba University Hospital, 1-8-1 Inohana, Chuo-ward, Chiba-city, Chiba 266-8677, Japan.
Nishimura M; Division of Laboratory Medicine, Chiba University Hospital, 1-8-1 Inohana, Chuo-ward, Chiba-city, Chiba 266-8677, Japan.
Igari H; Department of Infectious Diseases, Chiba University Hospital, 1-8-1 Inohana, Chuo-ward, Chiba-city, Chiba 266-8677, Japan.
Matsushita K; Division of Laboratory Medicine, Chiba University Hospital, 1-8-1 Inohana, Chuo-ward, Chiba-city, Chiba 266-8677, Japan.
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Źródło :
Clinica chimica acta; international journal of clinical chemistry [Clin Chim Acta] 2020 Aug; Vol. 507, pp. 139-142. Date of Electronic Publication: 2020 Apr 23.
Typ publikacji :
Journal Article
MeSH Terms :
Multiplex Polymerase Chain Reaction*
Reverse Transcriptase Polymerase Chain Reaction*/methods
Reverse Transcriptase Polymerase Chain Reaction*/standards
Betacoronavirus/*genetics
Clinical Laboratory Techniques/*methods
Coronavirus Infections/*diagnosis
Coronavirus Infections/*genetics
Pneumonia, Viral/*diagnosis
RNA, Viral/*genetics
Betacoronavirus/isolation & purification ; COVID-19 ; COVID-19 Testing ; COVID-19 Vaccines ; Clinical Laboratory Techniques/standards ; Humans ; Pandemics ; Pneumonia, Viral/genetics ; RNA, Viral/isolation & purification ; Real-Time Polymerase Chain Reaction/methods ; Real-Time Polymerase Chain Reaction/standards ; Reproducibility of Results ; SARS-CoV-2 ; Sputum/chemistry ; Sputum/virology
Czasopismo naukowe
Tytuł :
Sensitive detection and quantification of SARS-CoV-2 by multiplex droplet digital RT-PCR.
Autorzy :
de Kock R; Clinical Laboratory, Catharina Hospital Eindhoven, Eindhoven, The Netherlands. .; Institute for Complex Molecular Systems and Department of Biomedical Engineering, Laboratory of Chemical Biology, Eindhoven University of Technology, Eindhoven, The Netherlands. .; Expert Center Clinical Chemistry Eindhoven, Eindhoven, The Netherlands. .
Baselmans M; Clinical Laboratory, Catharina Hospital Eindhoven, Eindhoven, The Netherlands.
Scharnhorst V; Clinical Laboratory, Catharina Hospital Eindhoven, Eindhoven, The Netherlands.; Institute for Complex Molecular Systems and Department of Biomedical Engineering, Laboratory of Chemical Biology, Eindhoven University of Technology, Eindhoven, The Netherlands.; Expert Center Clinical Chemistry Eindhoven, Eindhoven, The Netherlands.
Deiman B; Clinical Laboratory, Catharina Hospital Eindhoven, Eindhoven, The Netherlands.; Institute for Complex Molecular Systems and Department of Biomedical Engineering, Laboratory of Chemical Biology, Eindhoven University of Technology, Eindhoven, The Netherlands.; Expert Center Clinical Chemistry Eindhoven, Eindhoven, The Netherlands.
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Źródło :
European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology [Eur J Clin Microbiol Infect Dis] 2021 Apr; Vol. 40 (4), pp. 807-813. Date of Electronic Publication: 2020 Oct 26.
Typ publikacji :
Journal Article
MeSH Terms :
COVID-19/*diagnosis
COVID-19 Nucleic Acid Testing/*methods
DNA/*analysis
Multiplex Polymerase Chain Reaction/*methods
RNA, Messenger/*analysis
RNA, Viral/*analysis
Reverse Transcriptase Polymerase Chain Reaction/*methods
Autoantigens/genetics ; Coronavirus Envelope Proteins/genetics ; Coronavirus Nucleocapsid Proteins/genetics ; Coronavirus RNA-Dependent RNA Polymerase/genetics ; Genes, Essential ; Glucuronidase/genetics ; Humans ; Phosphoproteins/genetics ; Real-Time Polymerase Chain Reaction ; Ribonuclease P/genetics ; SARS-CoV-2 ; Sensitivity and Specificity
Czasopismo naukowe
Tytuł :
Rapid, Sensitive, and Specific Severe Acute Respiratory Syndrome Coronavirus 2 Detection: A Multicenter Comparison Between Standard Quantitative Reverse-Transcriptase Polymerase Chain Reaction and CRISPR-Based DETECTR.
Autorzy :
Brandsma E; Sanquin Research, Department of Hematopoiesis, Amsterdam, The Netherlands.; Landsteiner Laboratory, Amsterdam University Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
Verhagen HJMP; Sanquin Research, Department of Hematopoiesis, Amsterdam, The Netherlands.; Landsteiner Laboratory, Amsterdam University Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
van de Laar TJW; Landsteiner Laboratory, Amsterdam University Medical Center, University of Amsterdam, Amsterdam, The Netherlands.; Sanquin Research, Department of Donor Medicine Research, Amsterdam, The Netherlands.; Department of Medical Microbiology, Onze Lieve Vrouwe Gasthuis, Amsterdam, The Netherlands.
Claas ECJ; Department of Medical Microbiology, Leiden University Medical Center, Leiden, The Netherlands.
Cornelissen M; Department of Medical Microbiology, Amsterdam University Medical Center, Amsterdam, The Netherlands.
van den Akker E; Sanquin Research, Department of Hematopoiesis, Amsterdam, The Netherlands.; Landsteiner Laboratory, Amsterdam University Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
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Źródło :
The Journal of infectious diseases [J Infect Dis] 2021 Feb 03; Vol. 223 (2), pp. 206-213.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
COVID-19/*diagnosis
COVID-19/*virology
COVID-19 Testing/*methods
Reverse Transcriptase Polymerase Chain Reaction/*methods
SARS-CoV-2/*isolation & purification
Clinical Laboratory Techniques/methods ; Clustered Regularly Interspaced Short Palindromic Repeats ; Humans ; Molecular Diagnostic Techniques ; Nucleic Acid Amplification Techniques ; Point-of-Care Testing ; RNA, Viral/genetics ; Real-Time Polymerase Chain Reaction/methods ; Reference Standards ; Reproducibility of Results ; SARS-CoV-2/genetics
SCR Protocol :
LAMP assay
Czasopismo naukowe
Tytuł :
Evaluation of three extraction-free SARS-CoV-2 RT-PCR assays: A feasible alternative approach with low technical requirements.
Autorzy :
Visseaux B; Université de Paris, INSERM UMR 1137 IAME, Laboratoire de Virologie, AP-HP, Hôpital Bichat-Claude Bernard, F-75018 Paris, France.
Collin G; Université de Paris, INSERM UMR 1137 IAME, Laboratoire de Virologie, AP-HP, Hôpital Bichat-Claude Bernard, F-75018 Paris, France.
Houhou-Fidouh N; Université de Paris, INSERM UMR 1137 IAME, Laboratoire de Virologie, AP-HP, Hôpital Bichat-Claude Bernard, F-75018 Paris, France.
Le Hingrat Q; Université de Paris, INSERM UMR 1137 IAME, Laboratoire de Virologie, AP-HP, Hôpital Bichat-Claude Bernard, F-75018 Paris, France.
Ferré VM; Université de Paris, INSERM UMR 1137 IAME, Laboratoire de Virologie, AP-HP, Hôpital Bichat-Claude Bernard, F-75018 Paris, France.
Damond F; Université de Paris, INSERM UMR 1137 IAME, Laboratoire de Virologie, AP-HP, Hôpital Bichat-Claude Bernard, F-75018 Paris, France.
Ichou H; Université de Paris, INSERM UMR 1137 IAME, Laboratoire de Virologie, AP-HP, Hôpital Bichat-Claude Bernard, F-75018 Paris, France.
Descamps D; Université de Paris, INSERM UMR 1137 IAME, Laboratoire de Virologie, AP-HP, Hôpital Bichat-Claude Bernard, F-75018 Paris, France.
Charpentier C; Université de Paris, INSERM UMR 1137 IAME, Laboratoire de Virologie, AP-HP, Hôpital Bichat-Claude Bernard, F-75018 Paris, France. Electronic address: .
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Źródło :
Journal of virological methods [J Virol Methods] 2021 May; Vol. 291, pp. 114086. Date of Electronic Publication: 2021 Feb 09.
Typ publikacji :
Journal Article
MeSH Terms :
COVID-19/*diagnosis
COVID-19 Testing/*methods
Reverse Transcriptase Polymerase Chain Reaction/*methods
SARS-CoV-2/*genetics
SARS-CoV-2/*isolation & purification
COVID-19/virology ; Clinical Laboratory Techniques/methods ; RNA, Viral/genetics ; Reagent Kits, Diagnostic ; Real-Time Polymerase Chain Reaction/methods ; Sensitivity and Specificity ; Viral Load
Czasopismo naukowe
Tytuł :
TaqMan-MGB probe quantitative PCR assays to genotype and quantify three mtDNA mutations of Leber hereditary optic neuropathy.
Autorzy :
Xue B; Chongqing Key Laboratory of Child Infection and Immunity, Ministry of Education Key Laboratory of Child Development and Disorders, National Clinical Research Center for Child Health and Disorders, China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Children's Hospital of Chongqing Medical University, No. 136 Zhongshan Erd Road, Yuzhong District, Chongqing, 400014, China.
Li Y; Chongqing Key Laboratory of Child Infection and Immunity, Ministry of Education Key Laboratory of Child Development and Disorders, National Clinical Research Center for Child Health and Disorders, China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Children's Hospital of Chongqing Medical University, No. 136 Zhongshan Erd Road, Yuzhong District, Chongqing, 400014, China.; Department of Laboratory Medicine, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou , 450007, China.
Wang X; Chongqing Key Laboratory of Child Infection and Immunity, Ministry of Education Key Laboratory of Child Development and Disorders, National Clinical Research Center for Child Health and Disorders, China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Children's Hospital of Chongqing Medical University, No. 136 Zhongshan Erd Road, Yuzhong District, Chongqing, 400014, China.
Li R; Chongqing Key Laboratory of Child Infection and Immunity, Ministry of Education Key Laboratory of Child Development and Disorders, National Clinical Research Center for Child Health and Disorders, China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Children's Hospital of Chongqing Medical University, No. 136 Zhongshan Erd Road, Yuzhong District, Chongqing, 400014, China.
Zeng X; Chongqing Key Laboratory of Child Infection and Immunity, Ministry of Education Key Laboratory of Child Development and Disorders, National Clinical Research Center for Child Health and Disorders, China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Children's Hospital of Chongqing Medical University, No. 136 Zhongshan Erd Road, Yuzhong District, Chongqing, 400014, China.
Yang M; Department of Neurosurgery, Xinqiao Hospital of Army Military Medical University, Chongqing, 400037, China.
Xu X; Chongqing Key Laboratory of Child Infection and Immunity, Ministry of Education Key Laboratory of Child Development and Disorders, National Clinical Research Center for Child Health and Disorders, China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Children's Hospital of Chongqing Medical University, No. 136 Zhongshan Erd Road, Yuzhong District, Chongqing, 400014, China.
Ye T; Chongqing Key Laboratory of Child Infection and Immunity, Ministry of Education Key Laboratory of Child Development and Disorders, National Clinical Research Center for Child Health and Disorders, China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Children's Hospital of Chongqing Medical University, No. 136 Zhongshan Erd Road, Yuzhong District, Chongqing, 400014, China.; The Third People's Hospital of Chengdu, Chengdu, 610031, China.
Bao L; Department of Pathology, University of Colorado School of Medicine, Aurora, CO, 80045, USA.
Huang Y; Chongqing Key Laboratory of Child Infection and Immunity, Ministry of Education Key Laboratory of Child Development and Disorders, National Clinical Research Center for Child Health and Disorders, China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Children's Hospital of Chongqing Medical University, No. 136 Zhongshan Erd Road, Yuzhong District, Chongqing, 400014, China. .
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Źródło :
Scientific reports [Sci Rep] 2020 Jul 23; Vol. 10 (1), pp. 12264. Date of Electronic Publication: 2020 Jul 23.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
DNA, Mitochondrial*
Genetic Association Studies*
Genetic Predisposition to Disease*
Genotype*
Mutation*
Real-Time Polymerase Chain Reaction*/methods
Optic Atrophy, Hereditary, Leber/*diagnosis
Optic Atrophy, Hereditary, Leber/*genetics
Alleles ; Gene Frequency ; Genes, Mitochondrial ; Humans ; Multiplex Polymerase Chain Reaction ; RNA, Ribosomal/genetics
Czasopismo naukowe
Tytuł :
[Establishment and evaluation of multiplex PCR for detection of main pathogenic bacteria of endometritis in Tibetan sheep].
Autorzy :
Han J; Technology and Research Center of Gansu Province for Embryo Engineering of Bovine and Sheep& Goat, College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, Gansu, China.
Wang M; Technology and Research Center of Gansu Province for Embryo Engineering of Bovine and Sheep& Goat, College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, Gansu, China.
Pan Y; Technology and Research Center of Gansu Province for Embryo Engineering of Bovine and Sheep& Goat, College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, Gansu, China.
Hu X; Technology and Research Center of Gansu Province for Embryo Engineering of Bovine and Sheep& Goat, College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, Gansu, China.
Zhang X; Technology and Research Center of Gansu Province for Embryo Engineering of Bovine and Sheep& Goat, College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, Gansu, China.
Cui Y; Technology and Research Center of Gansu Province for Embryo Engineering of Bovine and Sheep& Goat, College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, Gansu, China.
Xu G; Technology and Research Center of Gansu Province for Embryo Engineering of Bovine and Sheep& Goat, College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, Gansu, China.
Wang L; Technology and Research Center of Gansu Province for Embryo Engineering of Bovine and Sheep& Goat, College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, Gansu, China.
Yu S; Technology and Research Center of Gansu Province for Embryo Engineering of Bovine and Sheep& Goat, College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, Gansu, China.
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Źródło :
Sheng wu gong cheng xue bao = Chinese journal of biotechnology [Sheng Wu Gong Cheng Xue Bao] 2020 May 25; Vol. 36 (5), pp. 908-919.
Typ publikacji :
Evaluation Study; Journal Article
MeSH Terms :
Bacteria*/genetics
Bacteria*/isolation & purification
Bacteriological Techniques*/methods
Endometritis*/microbiology
Endometritis*/veterinary
Multiplex Polymerase Chain Reaction*/standards
Sheep Diseases*/microbiology
Animals ; Female ; Polymerase Chain Reaction/veterinary ; Sensitivity and Specificity ; Sheep ; Tibet
Czasopismo naukowe
Tytuł :
A new SYBR Green real-time PCR to detect SARS-CoV-2.
Autorzy :
Marinowic DR; Brain Institute of Rio Grande do Sul (BraIns), Pontifical Catholic University of Rio Grande do Sul, Av Ipiranga, 6690, Building 63, Jardim Botânico, Porto Alegre, Rio Grande do Sul, 90610.000, Brazil.; Graduate Program in Medicine and Health Sciences, Pontifical Catholic University of Rio Grande do Sul, Porto Alegre, Brazil.; Graduate Program in Medicine, Pediatrics and Child Health, Pontifical Catholic University of Rio Grande do Sul, Porto Alegre, Brazil.; Graduate Program in Biomedical Gerontology, Pontifical Catholic University of Rio Grande do Sul, Porto Alegre, Brazil.; School of Medicine, Pontifical Catholic University of Rio Grande do Sul, Porto Alegre, Brazil.
Zanirati G; Brain Institute of Rio Grande do Sul (BraIns), Pontifical Catholic University of Rio Grande do Sul, Av Ipiranga, 6690, Building 63, Jardim Botânico, Porto Alegre, Rio Grande do Sul, 90610.000, Brazil.; Graduate Program in Medicine, Pediatrics and Child Health, Pontifical Catholic University of Rio Grande do Sul, Porto Alegre, Brazil.
Rodrigues FVF; Brain Institute of Rio Grande do Sul (BraIns), Pontifical Catholic University of Rio Grande do Sul, Av Ipiranga, 6690, Building 63, Jardim Botânico, Porto Alegre, Rio Grande do Sul, 90610.000, Brazil.; Graduate Program in Medicine, Pediatrics and Child Health, Pontifical Catholic University of Rio Grande do Sul, Porto Alegre, Brazil.
Grahl MVC; Brain Institute of Rio Grande do Sul (BraIns), Pontifical Catholic University of Rio Grande do Sul, Av Ipiranga, 6690, Building 63, Jardim Botânico, Porto Alegre, Rio Grande do Sul, 90610.000, Brazil.; Graduate Program in Medicine and Health Sciences, Pontifical Catholic University of Rio Grande do Sul, Porto Alegre, Brazil.
Alcará AM; Brain Institute of Rio Grande do Sul (BraIns), Pontifical Catholic University of Rio Grande do Sul, Av Ipiranga, 6690, Building 63, Jardim Botânico, Porto Alegre, Rio Grande do Sul, 90610.000, Brazil.; Graduate Program in Medicine, Pediatrics and Child Health, Pontifical Catholic University of Rio Grande do Sul, Porto Alegre, Brazil.
Machado DC; Brain Institute of Rio Grande do Sul (BraIns), Pontifical Catholic University of Rio Grande do Sul, Av Ipiranga, 6690, Building 63, Jardim Botânico, Porto Alegre, Rio Grande do Sul, 90610.000, Brazil.; Graduate Program in Medicine and Health Sciences, Pontifical Catholic University of Rio Grande do Sul, Porto Alegre, Brazil.; Graduate Program in Biomedical Gerontology, Pontifical Catholic University of Rio Grande do Sul, Porto Alegre, Brazil.; School of Medicine, Pontifical Catholic University of Rio Grande do Sul, Porto Alegre, Brazil.
Da Costa JC; Brain Institute of Rio Grande do Sul (BraIns), Pontifical Catholic University of Rio Grande do Sul, Av Ipiranga, 6690, Building 63, Jardim Botânico, Porto Alegre, Rio Grande do Sul, 90610.000, Brazil. .; Graduate Program in Medicine and Health Sciences, Pontifical Catholic University of Rio Grande do Sul, Porto Alegre, Brazil. .; Graduate Program in Medicine, Pediatrics and Child Health, Pontifical Catholic University of Rio Grande do Sul, Porto Alegre, Brazil. .; School of Medicine, Pontifical Catholic University of Rio Grande do Sul, Porto Alegre, Brazil. .
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Źródło :
Scientific reports [Sci Rep] 2021 Jan 26; Vol. 11 (1), pp. 2224. Date of Electronic Publication: 2021 Jan 26.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Organic Chemicals*
SARS-CoV-2*
COVID-19/*diagnosis
COVID-19 Nucleic Acid Testing/*methods
Real-Time Polymerase Chain Reaction/*methods
DNA, Single-Stranded ; Enterovirus ; Genome, Viral ; Humans ; Phylogeny ; Polymerase Chain Reaction ; Rhinovirus ; Sensitivity and Specificity ; Temperature ; Viral Load
Czasopismo naukowe
Tytuł :
A Novel Approach to the Bioluminescent Detection of the SARS-CoV-2 ORF1ab Gene by Coupling Isothermal RNA Reverse Transcription Amplification with a Digital PCR Approach.
Autorzy :
Fei Z; State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.
Wei R; State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.
Cheng C; State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.
Xiao P; State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.
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Źródło :
International journal of molecular sciences [Int J Mol Sci] 2021 Jan 20; Vol. 22 (3). Date of Electronic Publication: 2021 Jan 20.
Typ publikacji :
Journal Article
MeSH Terms :
COVID-19/*virology
Molecular Diagnostic Techniques/*methods
Nucleic Acid Amplification Techniques/*methods
RNA, Viral/*genetics
Reverse Transcriptase Polymerase Chain Reaction/*methods
SARS-CoV-2/*genetics
Viral Proteins/*genetics
Humans ; Limit of Detection ; Luminescent Measurements/methods ; Polyproteins/genetics ; RNA, Viral/analysis ; Real-Time Polymerase Chain Reaction/methods ; Reverse Transcription
SCR Protocol :
LAMP assay
Czasopismo naukowe
Tytuł :
Developing multiplex ddPCR assays for SARS-CoV-2 detection based on probe mix and amplitude based multiplexing.
Autorzy :
Nyaruaba R; Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, Hubei, China.; International College, University of Chinese Academy of Sciences, Beijing, China.; Microbiology, Sino-Africa Joint Research Center, Nairobi, Kenya.
Li C; Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, Hubei, China.; International College, University of Chinese Academy of Sciences, Beijing, China.
Mwaliko C; Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, Hubei, China.; International College, University of Chinese Academy of Sciences, Beijing, China.; Microbiology, Sino-Africa Joint Research Center, Nairobi, Kenya.
Mwau M; Center for Infectious and Parasitic Diseases Control Research, Kenya Medical Research Institute, Busia, Kenya.
Odiwuor N; Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, Hubei, China.; International College, University of Chinese Academy of Sciences, Beijing, China.; Microbiology, Sino-Africa Joint Research Center, Nairobi, Kenya.
Muturi E; Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, Hubei, China.; International College, University of Chinese Academy of Sciences, Beijing, China.; Microbiology, Sino-Africa Joint Research Center, Nairobi, Kenya.
Muema C; Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, Hubei, China.; International College, University of Chinese Academy of Sciences, Beijing, China.; Microbiology, Sino-Africa Joint Research Center, Nairobi, Kenya.
Xiong J; Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, Hubei, China.
Li J; Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, Hubei, China.
Yu J; Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, Hubei, China.
Wei H; Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, Hubei, China.
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Źródło :
Expert review of molecular diagnostics [Expert Rev Mol Diagn] 2021 Jan; Vol. 21 (1), pp. 119-129. Date of Electronic Publication: 2020 Dec 30.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
COVID-19 Nucleic Acid Testing*
COVID-19/*diagnosis
Multiplex Polymerase Chain Reaction/*methods
SARS-CoV-2/*isolation & purification
Antiviral Agents/pharmacology ; DNA Primers/genetics ; False Positive Reactions ; Humans ; Limit of Detection ; Pandemics ; RNA, Viral/isolation & purification ; Real-Time Polymerase Chain Reaction/methods ; Reproducibility of Results ; Sensitivity and Specificity ; Temperature ; Viral Load/methods
Czasopismo naukowe
Tytuł :
Blood markers (lymphocyte percentages, neutrophils, CRP and ESR) can help in prioritizing rRT-PCR test for suspected COVID-19 patients in countries with limited health resources.
Autorzy :
Ibnouf AO; Department of Medical Laboratory Sciences, Port Sudan Ahlia College, Port Sudan, Sudan.
Khalil MH; Department of Medical Laboratory Sciences, Omdurman Islamic University, Omdurman, Sudan.
Khalid R; Department of Clinical Genetics, Alneelain Stem Cells Centre, Alneelain University, Khartoum, Sudan.
Elshibli EM; Department of Biostatistics, Assafa Academy, Khartoum, Sudan.
Elsayed O; Department of Medicine, Faculty of Medicine, Alneelain University, Khartoum, Sudan.
Fadl-Elmula I; Department of Clinical Genetics, Alneelain Stem Cells Centre, Alneelain University, Khartoum, Sudan.
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Źródło :
The Pan African medical journal [Pan Afr Med J] 2020 Dec 10; Vol. 37, pp. 331. Date of Electronic Publication: 2020 Dec 10 (Print Publication: 2020).
Typ publikacji :
Journal Article
MeSH Terms :
COVID-19 Testing*
COVID-19/*diagnosis
Reverse Transcriptase Polymerase Chain Reaction/*methods
Adolescent ; Adult ; Aged ; Aged, 80 and over ; Biomarkers/blood ; Blood Sedimentation ; C-Reactive Protein/metabolism ; COVID-19/blood ; Child ; Female ; Humans ; Lymphocytes/metabolism ; Male ; Middle Aged ; Neutrophils/metabolism ; Real-Time Polymerase Chain Reaction ; Sudan ; Young Adult
Czasopismo naukowe
Tytuł :
A recombinase polymerase amplification-based lateral flow strip assay for rapid detection of genogroup II noroviruses in the field.
Autorzy :
Jia T; College of Food Science and Technology, Shanghai Ocean University, Shanghai, China.
Yu Y; College of Food Science and Technology, Shanghai Ocean University, Shanghai, China. .
Wang Y; College of Food Science and Technology, Shanghai Ocean University, Shanghai, China. .; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China. .; Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture, Shanghai, China. .
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Źródło :
Archives of virology [Arch Virol] 2020 Dec; Vol. 165 (12), pp. 2767-2776. Date of Electronic Publication: 2020 Sep 19.
Typ publikacji :
Journal Article
MeSH Terms :
Caliciviridae Infections/*diagnosis
Norovirus/*isolation & purification
Reverse Transcriptase Polymerase Chain Reaction/*methods
Caliciviridae Infections/genetics ; Feces/virology ; Humans ; Norovirus/genetics ; Real-Time Polymerase Chain Reaction/methods ; Recombinases/chemistry ; Sensitivity and Specificity
Czasopismo naukowe
Tytuł :
Evaluation of COVID-19 RT-qPCR Test in Multi sample Pools.
Autorzy :
Yelin I; Faculty of Biology, Technion - Israel Institute of Technology, Haifa, Israel.
Aharony N; Faculty of Biology, Technion - Israel Institute of Technology, Haifa, Israel.
Tamar ES; Faculty of Biology, Technion - Israel Institute of Technology, Haifa, Israel.
Argoetti A; Faculty of Biology, Technion - Israel Institute of Technology, Haifa, Israel.
Messer E; Safety Unit, Technion - Israel Institute of Technology, Haifa, Israel.
Berenbaum D; Faculty of Biology, Technion - Israel Institute of Technology, Haifa, Israel.
Shafran E; Virology laboratory, Rambam Health Care Campus, Haifa, Israel.
Kuzli A; Virology laboratory, Rambam Health Care Campus, Haifa, Israel.
Gandali N; Virology laboratory, Rambam Health Care Campus, Haifa, Israel.
Shkedi O; Faculty of Medicine, Technion - Israel Institute of Technology, Haifa, Israel.
Hashimshony T; Faculty of Biology, Technion - Israel Institute of Technology, Haifa, Israel.
Mandel-Gutfreund Y; Faculty of Biology, Technion - Israel Institute of Technology, Haifa, Israel.; Computer Science Department, Technion - Israel Institute of Technology, Haifa, Israel.
Halberthal M; Faculty of Medicine, Technion - Israel Institute of Technology, Haifa, Israel.; Rambam Health Care Campus, Haifa, Israel.
Geffen Y; Bacteriology laboratory, Rambam Health Care Campus, Haifa, Israel.
Szwarcwort-Cohen M; Virology laboratory, Rambam Health Care Campus, Haifa, Israel.
Kishony R; Faculty of Biology, Technion - Israel Institute of Technology, Haifa, Israel.; Computer Science Department, Technion - Israel Institute of Technology, Haifa, Israel.
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Źródło :
Clinical infectious diseases : an official publication of the Infectious Diseases Society of America [Clin Infect Dis] 2020 Nov 19; Vol. 71 (16), pp. 2073-2078.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
COVID-19/*diagnosis
Real-Time Polymerase Chain Reaction/*methods
COVID-19/virology ; Humans ; Reverse Transcriptase Polymerase Chain Reaction ; SARS-CoV-2/genetics ; SARS-CoV-2/pathogenicity
Czasopismo naukowe
Tytuł :
Affine analysis for quantitative PCR measurements.
Autorzy :
Patrone PN; National Institute of Standards and Technology, 100 Bureau Drive, Gaithersburg, MD, 20899, USA. .
Romsos EL; National Institute of Standards and Technology, 100 Bureau Drive, Gaithersburg, MD, 20899, USA.
Cleveland MH; National Institute of Standards and Technology, 100 Bureau Drive, Gaithersburg, MD, 20899, USA.
Vallone PM; National Institute of Standards and Technology, 100 Bureau Drive, Gaithersburg, MD, 20899, USA.
Kearsley AJ; National Institute of Standards and Technology, 100 Bureau Drive, Gaithersburg, MD, 20899, USA.
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Źródło :
Analytical and bioanalytical chemistry [Anal Bioanal Chem] 2020 Nov; Vol. 412 (28), pp. 7977-7988. Date of Electronic Publication: 2020 Sep 20.
Typ publikacji :
Journal Article
MeSH Terms :
Algorithms*
Betacoronavirus/*genetics
Coronavirus Infections/*virology
Pneumonia, Viral/*virology
RNA, Viral/*genetics
Reverse Transcriptase Polymerase Chain Reaction/*methods
Betacoronavirus/isolation & purification ; COVID-19 ; Coronavirus Infections/diagnosis ; Humans ; Pandemics ; Pneumonia, Viral/diagnosis ; RNA, Viral/analysis ; Real-Time Polymerase Chain Reaction/methods ; SARS-CoV-2
Czasopismo naukowe
Tytuł :
Development of a Novel, Genome Subtraction-Derived, SARS-CoV-2-Specific COVID-19-nsp2 Real-Time RT-PCR Assay and Its Evaluation Using Clinical Specimens.
Autorzy :
Yip CC; Department of Microbiology, Queen Mary Hospital, HKSAR, Hong Kong, China.
Ho CC; Genomics and Bioinformatics Programme, The Chinese University of Hong Kong, HKSAR, Hong Kong, China.
Chan JF; State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, HKSAR, Hong Kong, China.; Department of Microbiology, The University of Hong Kong, HKSAR, Hong Kong, China.; Department of Clinical Microbiology and Infection, The University of Hong Kong-Shenzhen Hospital, Shenzhen 518053, China.; Carol Yu Centre for Infection, Li Ka Shing Faculty of Medicine, The University of Hong Kong, HKSAR, Hong Kong, China.
To KK; State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, HKSAR, Hong Kong, China.; Department of Microbiology, The University of Hong Kong, HKSAR, Hong Kong, China.; Department of Clinical Microbiology and Infection, The University of Hong Kong-Shenzhen Hospital, Shenzhen 518053, China.; Carol Yu Centre for Infection, Li Ka Shing Faculty of Medicine, The University of Hong Kong, HKSAR, Hong Kong, China.
Chan HS; Department of Medicine, Queen Elizabeth Hospital, HKSAR, Hong Kong, China.
Wong SC; Department of Pathology, Queen Elizabeth Hospital, HKSAR, Hong Kong, China.
Leung KH; Department of Microbiology, The University of Hong Kong, HKSAR, Hong Kong, China.
Fung AY; Department of Microbiology, The University of Hong Kong, HKSAR, Hong Kong, China.
Ng AC; Department of Microbiology, The University of Hong Kong, HKSAR, Hong Kong, China.
Zou Z; Department of Microbiology, The University of Hong Kong, HKSAR, Hong Kong, China.
Tam AR; Department of Medicine, Queen Mary Hospital, HKSAR, Hong Kong, China.
Chung TW; Department of Microbiology, Queen Mary Hospital, HKSAR, Hong Kong, China.
Chan KH; State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, HKSAR, Hong Kong, China.; Department of Microbiology, The University of Hong Kong, HKSAR, Hong Kong, China.; Carol Yu Centre for Infection, Li Ka Shing Faculty of Medicine, The University of Hong Kong, HKSAR, Hong Kong, China.
Hung IF; Department of Medicine, The University of Hong Kong, HKSAR, Hong Kong, China.
Cheng VC; Department of Microbiology, Queen Mary Hospital, HKSAR, Hong Kong, China.
Tsang OT; Department of Medicine and Geriatrics, Princess Margaret Hospital, HKSAR, Hong Kong, China.
Tsui SKW; School of Biomedical Sciences, The Chinese University of Hong Kong, HKSAR, Hong Kong, China.
Yuen KY; State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, HKSAR, Hong Kong, China.; Department of Microbiology, The University of Hong Kong, HKSAR, Hong Kong, China.; Department of Clinical Microbiology and Infection, The University of Hong Kong-Shenzhen Hospital, Shenzhen 518053, China.; Carol Yu Centre for Infection, Li Ka Shing Faculty of Medicine, The University of Hong Kong, HKSAR, Hong Kong, China.
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Źródło :
International journal of molecular sciences [Int J Mol Sci] 2020 Apr 08; Vol. 21 (7). Date of Electronic Publication: 2020 Apr 08.
Typ publikacji :
Journal Article
MeSH Terms :
Genome, Viral*
Real-Time Polymerase Chain Reaction*
Betacoronavirus/*genetics
Clinical Laboratory Techniques/*methods
Coronavirus Infections/*diagnosis
Pneumonia, Viral/*diagnosis
RNA, Viral/*analysis
COVID-19 ; COVID-19 Testing ; COVID-19 Vaccines ; Humans ; Pandemics ; Reproducibility of Results ; Reverse Transcriptase Polymerase Chain Reaction ; SARS-CoV-2 ; Sensitivity and Specificity
Czasopismo naukowe
Tytuł :
High-quality RT-PCR with chemically modified RNA controls.
Autorzy :
Luo G; Key Laboratory of Bio-Resource and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan, 610064, PR China; Department of Clinical Laboratory, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan, China.
Zhang J; Key Laboratory of Bio-Resource and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan, 610064, PR China.
Zhang S; Key Laboratory of Bio-Resource and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan, 610064, PR China.
Hu B; Key Laboratory of Bio-Resource and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan, 610064, PR China.
Hu L; SeNA Research Institute, Atlanta, GA, 30303, USA.
Huang Z; Key Laboratory of Bio-Resource and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan, 610064, PR China; SeNA Research Institute, Atlanta, GA, 30303, USA. Electronic address: .
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Źródło :
Talanta [Talanta] 2021 Mar 01; Vol. 224, pp. 121850. Date of Electronic Publication: 2020 Nov 11.
Typ publikacji :
Journal Article
MeSH Terms :
COVID-19/*diagnosis
COVID-19 Nucleic Acid Testing/*methods
RNA, Viral/*analysis
Real-Time Polymerase Chain Reaction/*methods
Reverse Transcriptase Polymerase Chain Reaction/*methods
COVID-19 Nucleic Acid Testing/instrumentation ; DNA, Viral/analysis ; False Negative Reactions ; Humans ; RNA Stability ; RNA, Viral/chemistry ; Reagent Kits, Diagnostic ; Real-Time Polymerase Chain Reaction/instrumentation ; Reverse Transcriptase Polymerase Chain Reaction/instrumentation ; SARS-CoV-2/genetics
Czasopismo naukowe
Tytuł :
Development of a Droplet Digital PCR for Detection of Trichuriasis in Sheep.
Autorzy :
Yu Z; Department of Veterinary Medicine, College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang, 524088, Guangdong, China.; Technology Center, Hohhot Customs District, Hohhot, 010020, Inner Mongolia, China.
Zhao Z; Technology Center, Hohhot Customs District, Hohhot, 010020, Inner Mongolia, China.
Chen L; Technology Center, Hohhot Customs District, Hohhot, 010020, Inner Mongolia, China.
Li J; Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Hohhot, 010031, China.
Ju X; Department of Veterinary Medicine, College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang, 524088, Guangdong, China.
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Źródło :
The Journal of parasitology [J Parasitol] 2020 Oct 01; Vol. 106 (5), pp. 603-610.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Polymerase Chain Reaction/*veterinary
Sheep Diseases/*diagnosis
Trichuriasis/*veterinary
Animals ; DNA, Helminth/isolation & purification ; Feces/parasitology ; Polymerase Chain Reaction/methods ; Polymerase Chain Reaction/standards ; Real-Time Polymerase Chain Reaction/standards ; Real-Time Polymerase Chain Reaction/veterinary ; Reproducibility of Results ; Sensitivity and Specificity ; Sheep ; Sheep Diseases/parasitology ; Sheep Diseases/prevention & control ; Trichuriasis/diagnosis ; Trichuriasis/prevention & control
Czasopismo naukowe
Tytuł :
Design and Evaluation of a Novel Multiplex Real-Time PCR Melting Curve Assay for the Simultaneous Detection of Nine Sexually Transmitted Disease Pathogens in Genitourinary Secretions.
Autorzy :
Hu XM; Department of Laboratory Medicine, First Affiliated Hospital of Gannan Medical University, Ganzhou, China.; Department of Precision Medicine Center, First Affiliated Hospital of Gannan Medical University, Ganzhou, China.
Xu JX; Department of Medical Laboratory, The Fourth Affiliated Hospital of Nanchang University, Nanchang, China.
Jiang LX; Department of Laboratory Medicine, First Affiliated Hospital of Gannan Medical University, Ganzhou, China.
Deng LR; Department of Medical Laboratory, The Fourth Affiliated Hospital of Nanchang University, Nanchang, China.
Gu ZM; Department of Laboratory Medicine Center, Nanfang Hospital, Southern Medical University, Guangzhou, China.
Xie XY; Department of Obstetrics and Gynecology, First Affiliated Hospital of Gannan Medical University, Ganzhou, China.
Ji HC; Department of Laboratory Medicine Center, Nanfang Hospital, Southern Medical University, Guangzhou, China.
Wang WH; Department of Laboratory Medicine, First Affiliated Hospital of Gannan Medical University, Ganzhou, China.; Department of Precision Medicine Center, First Affiliated Hospital of Gannan Medical University, Ganzhou, China.
Li LM; Department of Dermatology, First Affiliated Hospital of Gannan Medical University, Ganzhou, China.
Tian CN; Department of Cardiac and Thoracic Surgery, First Affiliated Hospital of Gannan Medical University, Ganzhou, China.
Song FL; Jiangxi Shiningmed Medical Technology Ltd., Ganzhou, China.
Huang S; Jiangxi Shiningmed Medical Technology Ltd., Ganzhou, China.
Zheng L; Department of Laboratory Medicine Center, Nanfang Hospital, Southern Medical University, Guangzhou, China.
Zhong TY; Department of Laboratory Medicine, First Affiliated Hospital of Gannan Medical University, Ganzhou, China.; Department of Precision Medicine Center, First Affiliated Hospital of Gannan Medical University, Ganzhou, China.
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Źródło :
Frontiers in cellular and infection microbiology [Front Cell Infect Microbiol] 2019 Nov 12; Vol. 9, pp. 382. Date of Electronic Publication: 2019 Nov 12 (Print Publication: 2019).
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Bodily Secretions*
Real-Time Polymerase Chain Reaction*/methods
Real-Time Polymerase Chain Reaction*/standards
Sexually Transmitted Diseases/*diagnosis
Sexually Transmitted Diseases/*etiology
Urogenital System/*metabolism
Female ; Humans ; Male ; Multiplex Polymerase Chain Reaction/methods ; Multiplex Polymerase Chain Reaction/standards ; Reproducibility of Results ; Sensitivity and Specificity
Czasopismo naukowe
Tytuł :
Simpler and faster Covid-19 testing: Strategies to streamline SARS-CoV-2 molecular assays.
Autorzy :
Panpradist N; Department of Bioengineering, University of Washington, Seattle, WA, United States; Global Health of Women, Adolescents, and Children (Global WACh), School of Public Health, University of Washington, Seattle, WA, United States.
Wang Q; Department of Bioengineering, University of Washington, Seattle, WA, United States.
Ruth PS; Department of Bioengineering, University of Washington, Seattle, WA, United States; Paul G. Allen School of Computer Science & Engineering, University of Washington, Seattle, WA, United States.
Kotnik JH; Department of Bioengineering, University of Washington, Seattle, WA, United States; Department of Family Medicine, University of Washington, Seattle, WA, United States.
Oreskovic AK; Department of Bioengineering, University of Washington, Seattle, WA, United States.
Miller A; Department of Bioengineering, University of Washington, Seattle, WA, United States.
Stewart SWA; Department of Bioengineering, University of Washington, Seattle, WA, United States; Center for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, WA, United States.
Vrana J; Department of Bioengineering, University of Washington, Seattle, WA, United States.
Han PD; Department of Genome Sciences, Seattle, WA, United States; Brotman Baty Institute for Precision Medicine, Seattle, WA, United States.
Beck IA; Center for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, WA, United States.
Starita LM; Department of Genome Sciences, Seattle, WA, United States; Brotman Baty Institute for Precision Medicine, Seattle, WA, United States.
Frenkel LM; Center for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, WA, United States; Departments of Global Health, Medicine, Paediatrics, and Laboratory Medicine, University of Washington, Seattle, WA, United States. Electronic address: .
Lutz BR; Department of Bioengineering, University of Washington, Seattle, WA, United States; Brotman Baty Institute for Precision Medicine, Seattle, WA, United States. Electronic address: .
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Źródło :
EBioMedicine [EBioMedicine] 2021 Feb; Vol. 64, pp. 103236. Date of Electronic Publication: 2021 Feb 12.
Typ publikacji :
Clinical Trial; Journal Article; Multicenter Study
MeSH Terms :
COVID-19*/diagnosis
COVID-19*/genetics
COVID-19 Nucleic Acid Testing*
Real-Time Polymerase Chain Reaction*
Reverse Transcriptase Polymerase Chain Reaction*
RNA, Viral/*genetics
SARS-CoV-2/*genetics
Humans ; Sensitivity and Specificity
Czasopismo naukowe
Tytuł :
Comparison of three TaqMan real-time reverse transcription-PCR assays in detecting SARS-CoV-2.
Autorzy :
Xiao Y; National Health Commission Key Laboratory of Systems Biology of Pathogens and Christophe Mérieux Laboratory, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100730, PR China.
Li Z; Key Laboratory of Medical Molecular Cell Biology of Shanxi Province, Institutes of Biomedical Sciences, Shanxi University, Taiyuan, 030006, PR China.
Wang X; National Health Commission Key Laboratory of Systems Biology of Pathogens and Christophe Mérieux Laboratory, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100730, PR China.
Wang Y; National Health Commission Key Laboratory of Systems Biology of Pathogens and Christophe Mérieux Laboratory, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100730, PR China.
Wang Y; National Health Commission Key Laboratory of Systems Biology of Pathogens and Christophe Mérieux Laboratory, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100730, PR China.
Wang G; National Health Commission Key Laboratory of Systems Biology of Pathogens and Christophe Mérieux Laboratory, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100730, PR China.
Ren L; National Health Commission Key Laboratory of Systems Biology of Pathogens and Christophe Mérieux Laboratory, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100730, PR China; Key Laboratory of Respiratory Disease Pathogenomics, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100730, PR China. Electronic address: .
Li J; Key Laboratory of Medical Molecular Cell Biology of Shanxi Province, Institutes of Biomedical Sciences, Shanxi University, Taiyuan, 030006, PR China; The Provincial Key Laboratories for Prevention and Treatment of Major Infectious Diseases, Shanxi, Taiyuan, 030006, PR China. Electronic address: .
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Źródło :
Journal of virological methods [J Virol Methods] 2021 Feb; Vol. 288, pp. 114030. Date of Electronic Publication: 2020 Dec 01.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Real-Time Polymerase Chain Reaction*/methods
Reverse Transcriptase Polymerase Chain Reaction*/methods
COVID-19/*diagnosis
COVID-19/*virology
COVID-19 Testing/*methods
SARS-CoV-2/*genetics
COVID-19 Testing/standards ; Humans ; Reproducibility of Results ; Sensitivity and Specificity
Czasopismo naukowe

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