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Wyszukujesz frazę ""Protein Engineering"" wg kryterium: Temat


Tytuł :
Optimizing the Expression of Human Dopamine Receptors in Escherichia coli .
Autorzy :
Boritzki V; Department of Biochemistry IV-Biopolymers, Universität Bayreuth, Universitätsstr. 30, 95447 Bayreuth, Germany.
Hübner H; Department of Chemistry and Pharmacy, Medicinal Chemistry, Friedrich-Alexander-Universität Erlangen-Nürnberg, Nikolaus-Fiebiger Str. 10, 91058 Erlangen, Germany.
Allikalt A; Department of Chemistry and Pharmacy, Medicinal Chemistry, Friedrich-Alexander-Universität Erlangen-Nürnberg, Nikolaus-Fiebiger Str. 10, 91058 Erlangen, Germany.
Gmeiner P; Department of Chemistry and Pharmacy, Medicinal Chemistry, Friedrich-Alexander-Universität Erlangen-Nürnberg, Nikolaus-Fiebiger Str. 10, 91058 Erlangen, Germany.
Wöhrl BM; Department of Biochemistry IV-Biopolymers, Universität Bayreuth, Universitätsstr. 30, 95447 Bayreuth, Germany.
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Źródło :
International journal of molecular sciences [Int J Mol Sci] 2021 Aug 11; Vol. 22 (16). Date of Electronic Publication: 2021 Aug 11.
Typ publikacji :
Journal Article
MeSH Terms :
Escherichia coli/*genetics
Protein Engineering/*methods
Receptors, Dopamine/*genetics
Calibration ; Cell Membrane/metabolism ; Cloning, Molecular/methods ; Escherichia coli/metabolism ; Gene Library ; Humans ; Mutation ; Organisms, Genetically Modified ; Protein Engineering/standards ; Receptors, Dopamine/metabolism ; Receptors, Dopamine D2/genetics ; Receptors, Dopamine D2/metabolism ; Receptors, Dopamine D3/genetics ; Receptors, Dopamine D3/metabolism ; Receptors, G-Protein-Coupled/genetics ; Receptors, G-Protein-Coupled/metabolism ; Recombinant Proteins/genetics ; Recombinant Proteins/metabolism ; Transformation, Bacterial ; Transgenes
Czasopismo naukowe
Tytuł :
Modification of DNA regions with metagenomic DNA fragments (MDRMDF): A convenient strategy for efficient protein engineering.
Autorzy :
Xu S; College of Food Science, Shenyang Agricultural University, Shenyang, China; College of Biosciences and Biotechnology, Shenyang Agricultural University, Shenyang, China.
Qi X; School of Food and Biological Engineering, Jiangsu University, Zhenjiang, China.
Gao S; College of Biosciences and Biotechnology, Shenyang Agricultural University, Shenyang, China.
Zhang Y; College of Food Science, Shenyang Agricultural University, Shenyang, China; College of Biosciences and Biotechnology, Shenyang Agricultural University, Shenyang, China.
Wang H; College of Food Science, Shenyang Agricultural University, Shenyang, China; College of Biosciences and Biotechnology, Shenyang Agricultural University, Shenyang, China.
Shao Y; College of Biosciences and Biotechnology, Shenyang Agricultural University, Shenyang, China.
Yang Y; College of Biosciences and Biotechnology, Shenyang Agricultural University, Shenyang, China.
An Y; College of Food Science, Shenyang Agricultural University, Shenyang, China; College of Biosciences and Biotechnology, Shenyang Agricultural University, Shenyang, China. Electronic address: .
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Źródło :
Biochimie [Biochimie] 2021 Aug; Vol. 187, pp. 75-81. Date of Electronic Publication: 2021 May 27.
Typ publikacji :
Journal Article
MeSH Terms :
Cloning, Molecular*
Metagenomics*
Protein Engineering*
DNA/*genetics
Recombinant Proteins/chemistry ; Recombinant Proteins/genetics
Czasopismo naukowe
Tytuł :
A cell-free nanobody engineering platform rapidly generates SARS-CoV-2 neutralizing nanobodies.
Autorzy :
Chen X; Klarman Cell Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA. .
Gentili M; Broad Institute of MIT and Harvard, Cambridge, MA, USA.
Hacohen N; Broad Institute of MIT and Harvard, Cambridge, MA, USA.; Department of Medicine, Harvard Medical School, Boston, MA, USA.; Center for Cancer Research, Massachusetts General Hospital, Boston, MA, USA.
Regev A; Klarman Cell Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA. .; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA. .; Howard Hughes Medical Institute, Chevy Chase, MD, USA. .; Genentech, 1 DNA Way, South San Francisco, CA, USA. .
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Źródło :
Nature communications [Nat Commun] 2021 Sep 17; Vol. 12 (1), pp. 5506. Date of Electronic Publication: 2021 Sep 17.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
MeSH Terms :
Protein Engineering*
Antibodies, Neutralizing/*immunology
SARS-CoV-2/*drug effects
Single-Domain Antibodies/*chemistry
Single-Domain Antibodies/*pharmacology
Antibodies, Viral ; COVID-19/drug therapy ; Humans ; Protein Binding ; Reproducibility of Results ; Single-Domain Antibodies/genetics ; Spike Glycoprotein, Coronavirus
Czasopismo naukowe
Tytuł :
Engineering a carbohydrate binding module to enhance chitinase catalytic efficiency on insoluble chitinous substrate.
Autorzy :
Su H; College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.
Gao L; College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.
Sun J; College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.
Mao X; College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China; Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China. Electronic address: .
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Źródło :
Food chemistry [Food Chem] 2021 Sep 01; Vol. 355, pp. 129462. Date of Electronic Publication: 2021 Mar 16.
Typ publikacji :
Journal Article
MeSH Terms :
Protein Engineering*
Bacterial Proteins/*metabolism
Chitin/*metabolism
Chitinases/*metabolism
Amino Acid Sequence ; Bacillus/enzymology ; Bacterial Proteins/chemistry ; Bacterial Proteins/genetics ; Binding Sites ; Chitinases/chemistry ; Chitinases/genetics ; Hydrolysis ; Molecular Dynamics Simulation ; Protein Structure, Tertiary ; Recombinant Proteins/biosynthesis ; Recombinant Proteins/isolation & purification ; Substrate Specificity
SCR Organism :
Bacillus circulans
Czasopismo naukowe
Tytuł :
Toward scalable biocatalytic conversion of 5-hydroxymethylfurfural by galactose oxidase using coordinated reaction and enzyme engineering.
Autorzy :
Birmingham WR; School of Chemistry, The University of Manchester, Manchester Institute of Biotechnology, Manchester, UK.
Toftgaard Pedersen A; Department of Chemical and Biochemical Engineering, Technical University of Denmark, Lyngby, Denmark.; Novozymes A/S, Krogshoejvej 36, Bagsvaerd, Denmark.
Dias Gomes M; Department of Chemical and Biochemical Engineering, Technical University of Denmark, Lyngby, Denmark.
Bøje Madsen M; Department of Chemical and Biochemical Engineering, Technical University of Denmark, Lyngby, Denmark.
Breuer M; BASF SE, White Biotechnology Research, Ludwigshafen, Germany.
Woodley JM; Department of Chemical and Biochemical Engineering, Technical University of Denmark, Lyngby, Denmark.
Turner NJ; School of Chemistry, The University of Manchester, Manchester Institute of Biotechnology, Manchester, UK. .
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Źródło :
Nature communications [Nat Commun] 2021 Aug 16; Vol. 12 (1), pp. 4946. Date of Electronic Publication: 2021 Aug 16.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Protein Engineering*
Furaldehyde/*analogs & derivatives
Furaldehyde/*metabolism
Galactose Oxidase/*genetics
Galactose Oxidase/*metabolism
Biocatalysis ; Catalysis ; Catalytic Domain ; Furans ; Galactose Oxidase/chemistry ; Mutagenesis ; Oxidation-Reduction
Czasopismo naukowe
Tytuł :
Molecular engineering of antimicrobial peptide (AMP)-polymer conjugates.
Autorzy :
Cui Z; Department of Chemical Engineering, University of Virginia, Charlottesville, VA, 22903, USA. .
Luo Q
Bannon MS
Gray VP
Bloom TG
Clore MF
Hughes MA
Crawford MA
Letteri RA
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Źródło :
Biomaterials science [Biomater Sci] 2021 Aug 07; Vol. 9 (15), pp. 5069-5091. Date of Electronic Publication: 2021 Jun 07.
Typ publikacji :
Journal Article; Review
MeSH Terms :
Anti-Infective Agents*/pharmacology
Polymers*
Protein Engineering*
Animals ; Anti-Bacterial Agents/pharmacology ; Antimicrobial Cationic Peptides ; Microbial Sensitivity Tests ; Pore Forming Cytotoxic Proteins
Czasopismo naukowe
Tytuł :
Immunoengineering approaches for cytokine therapy.
Autorzy :
Mansurov A; Pritzker School of Molecular Engineering, University of Chicago, Chicago, Illinois.
Lauterbach A; Pritzker School of Molecular Engineering, University of Chicago, Chicago, Illinois.
Budina E; Pritzker School of Molecular Engineering, University of Chicago, Chicago, Illinois.
Alpar AT; Pritzker School of Molecular Engineering, University of Chicago, Chicago, Illinois.
Hubbell JA; Pritzker School of Molecular Engineering, University of Chicago, Chicago, Illinois.
Ishihara J; Pritzker School of Molecular Engineering, University of Chicago, Chicago, Illinois.; Department of Bioengineering, Imperial College London, London, United Kingdom.
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Źródło :
American journal of physiology. Cell physiology [Am J Physiol Cell Physiol] 2021 Aug 01; Vol. 321 (2), pp. C369-C383. Date of Electronic Publication: 2021 Jul 07.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Immunotherapy*/methods
Protein Engineering*
Cytokines/*therapeutic use
Neoplasms/*therapy
Animals ; Cytokines/genetics ; Drug Delivery Systems/methods ; Humans ; Inflammation/drug therapy ; Neoplasms/genetics ; Neoplasms/immunology
Czasopismo naukowe
Tytuł :
Linker Engineering in the Context of Synthetic Protein Switches and Sensors.
Autorzy :
Gräwe A; Department of Biology, TU Darmstadt, 64287 Darmstadt, Germany; Centre for Synthetic Biology, TU Darmstadt, 64283 Darmstadt, Germany.
Stein V; Department of Biology, TU Darmstadt, 64287 Darmstadt, Germany; Centre for Synthetic Biology, TU Darmstadt, 64283 Darmstadt, Germany. Electronic address: .
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Źródło :
Trends in biotechnology [Trends Biotechnol] 2021 Jul; Vol. 39 (7), pp. 731-744. Date of Electronic Publication: 2020 Dec 05.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't; Review
MeSH Terms :
Protein Engineering*/trends
Proteins*/genetics
Amino Acid Motifs ; Biophysics ; Biosensing Techniques/trends ; Synthetic Biology
Czasopismo naukowe
Tytuł :
Engineering of a critical membrane-anchored enzyme for high solubility and catalytic activity.
Autorzy :
Hussain MS; Department of Chemistry & Biochemistry, University of Toledo, Toledo, OH, 43606, USA.
Wang Q; Department of Chemistry & Biochemistry, University of Toledo, Toledo, OH, 43606, USA.
Viola RE; Department of Chemistry & Biochemistry, University of Toledo, Toledo, OH, 43606, USA. Electronic address: .
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Źródło :
Archives of biochemistry and biophysics [Arch Biochem Biophys] 2021 May 30; Vol. 703, pp. 108870. Date of Electronic Publication: 2021 Apr 05.
Typ publikacji :
Journal Article
MeSH Terms :
Biocatalysis*
Protein Engineering*
Acetyltransferases/*genetics
Acetyltransferases/*metabolism
Cell Membrane/*enzymology
Acetyltransferases/chemistry ; Amino Acid Sequence ; Aspartic Acid/analogs & derivatives ; Aspartic Acid/metabolism ; Cell Line ; Humans ; Protein Domains ; Solubility
Czasopismo naukowe
Tytuł :
Design and construction of novel biocatalyst for bioprocessing: Recent advances and future outlook.
Autorzy :
Xu Y; Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, PR China; Science Center for Future Foods, Jiangnan University, Wuxi 214122, PR China.
Wu Y; Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, PR China; Science Center for Future Foods, Jiangnan University, Wuxi 214122, PR China.
Lv X; Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, PR China; Science Center for Future Foods, Jiangnan University, Wuxi 214122, PR China.
Sun G; Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, PR China; Science Center for Future Foods, Jiangnan University, Wuxi 214122, PR China.
Zhang H; Shandong Runde Biotechnology Co., Ltd., Tai'an 271000, PR China.
Chen T; Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, PR China; Science Center for Future Foods, Jiangnan University, Wuxi 214122, PR China.
Du G; Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, PR China; Science Center for Future Foods, Jiangnan University, Wuxi 214122, PR China.
Li J; Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, PR China; Science Center for Future Foods, Jiangnan University, Wuxi 214122, PR China.
Liu L; Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, PR China; Science Center for Future Foods, Jiangnan University, Wuxi 214122, PR China. Electronic address: .
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Źródło :
Bioresource technology [Bioresour Technol] 2021 Jul; Vol. 332, pp. 125071. Date of Electronic Publication: 2021 Mar 31.
Typ publikacji :
Journal Article; Review
MeSH Terms :
Metabolic Engineering*
Protein Engineering*
Biocatalysis ; Industry
Czasopismo naukowe
Tytuł :
Surface charged amino acid-based strategy for rational engineering of kinetic stability and specific activity of enzymes: Linking experiments with computational modeling.
Autorzy :
Liu Z; College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.
Fu X; College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.
Yuan M; College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.
Liang Q; College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.
Zhu C; College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.
Mou H; College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China. Electronic address: .
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Źródło :
International journal of biological macromolecules [Int J Biol Macromol] 2021 Jul 01; Vol. 182, pp. 228-236. Date of Electronic Publication: 2021 Apr 05.
Typ publikacji :
Journal Article
MeSH Terms :
Catalytic Domain*
Molecular Dynamics Simulation*
Protein Engineering*
beta-Mannosidase/*metabolism
Enzyme Stability ; Hot Temperature ; Kinetics ; Protein Conformation ; Thermodynamics ; beta-Mannosidase/chemistry
Czasopismo naukowe
Tytuł :
[Strategies for engineering the thermo-stability of glycosidase].
Autorzy :
Liu R; School of Life Sciences, Yan'an University, Yan'an 716000, Shaanxi, China.
Liu Y; School of Life Sciences, Yan'an University, Yan'an 716000, Shaanxi, China.
Li Q; Beijing Honghui New Pharmaceutical Technology Limited Company, Beijing 102629, China.; Institute of Biochemical Engineering, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 100081, China.
Feng X; Institute of Biochemical Engineering, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 100081, China.
Li C; School of Life Sciences, Yan'an University, Yan'an 716000, Shaanxi, China.; Institute of Biochemical Engineering, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 100081, China.
Gao X; School of Life Sciences, Yan'an University, Yan'an 716000, Shaanxi, China.; Institute of Biochemical Engineering, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 100081, China.; Shaanxi Engineering and Technological Research Center for Conversation and Utilization of Regional Biological Resources, Yan'an 716000, Shaanxi, China.
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Źródło :
Sheng wu gong cheng xue bao = Chinese journal of biotechnology [Sheng Wu Gong Cheng Xue Bao] 2021 Jun 25; Vol. 37 (6), pp. 1919-1930.
Typ publikacji :
Journal Article; Review
MeSH Terms :
Glycoside Hydrolases*/genetics
Protein Engineering*
Oligosaccharides ; Polysaccharides
Czasopismo naukowe
Tytuł :
[Advances in engineering non-ribosomal peptide synthetase].
Autorzy :
Wang C; Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Xu Y; Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
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Źródło :
Sheng wu gong cheng xue bao = Chinese journal of biotechnology [Sheng Wu Gong Cheng Xue Bao] 2021 Jun 25; Vol. 37 (6), pp. 1845-1857.
Typ publikacji :
Journal Article
MeSH Terms :
Biological Products*
Peptide Synthases*/genetics
Protein Engineering*
Peptides
Czasopismo naukowe
Tytuł :
Structural-Based Modeling in Protein Engineering. A Must Do.
Autorzy :
Roda S; Barcelona Supercomputing Center (BSC), Barcelona 08034, Spain.
Robles-Martín A; Barcelona Supercomputing Center (BSC), Barcelona 08034, Spain.
Xiang R; Barcelona Supercomputing Center (BSC), Barcelona 08034, Spain.
Kazemi M; Barcelona Supercomputing Center (BSC), Barcelona 08034, Spain.
Guallar V; Barcelona Supercomputing Center (BSC), Barcelona 08034, Spain.; Institució Catalana de Recerca i Estudis Avançats (ICREA), Barcelona 08010, Spain.
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Źródło :
The journal of physical chemistry. B [J Phys Chem B] 2021 Jun 24; Vol. 125 (24), pp. 6491-6500. Date of Electronic Publication: 2021 Jun 09.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Computational Biology*
Protein Engineering*
Czasopismo naukowe
Tytuł :
Improvement of Biophysical Properties and Affinity of a Human Anti-L1CAM Therapeutic Antibody through Antibody Engineering Based on Computational Methods.
Autorzy :
Chae H; Department of Systems Immunology, Kangwon National University, Chuncheon 24341, Korea.; APIT BIO Inc., B910, Munjeongdong Tera Tower, 167 Songpa-daero, Songpa-gu, Seoul 05855, Korea.
Cho S; Institute of Bioscience and Biotechnology, Kangwon National University, Chuncheon 24341, Korea.
Jeong M; Department of Systems Immunology, Kangwon National University, Chuncheon 24341, Korea.
Kwon K; Department of Systems Immunology, Kangwon National University, Chuncheon 24341, Korea.
Choi D; Division of Drug Process Development, New Drug Development Center, Osong Medical Innovation Foundation, Chungcheongbuk-do, Cheongju-si 28160, Korea.
Lee J; APIT BIO Inc., B910, Munjeongdong Tera Tower, 167 Songpa-daero, Songpa-gu, Seoul 05855, Korea.
Nam W; APIT BIO Inc., B910, Munjeongdong Tera Tower, 167 Songpa-daero, Songpa-gu, Seoul 05855, Korea.
Hong J; Department of Systems Immunology, Kangwon National University, Chuncheon 24341, Korea.
Lee J; Department of Systems Immunology, Kangwon National University, Chuncheon 24341, Korea.
Yoon S; APIT BIO Inc., B910, Munjeongdong Tera Tower, 167 Songpa-daero, Songpa-gu, Seoul 05855, Korea.
Hong H; Department of Systems Immunology, Kangwon National University, Chuncheon 24341, Korea.; Institute of Bioscience and Biotechnology, Kangwon National University, Chuncheon 24341, Korea.
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Źródło :
International journal of molecular sciences [Int J Mol Sci] 2021 Jun 22; Vol. 22 (13). Date of Electronic Publication: 2021 Jun 22.
Typ publikacji :
Journal Article
MeSH Terms :
Models, Molecular*
Protein Engineering*/methods
Antibodies, Monoclonal/*chemistry
Neural Cell Adhesion Molecule L1/*chemistry
Animals ; Antibodies, Monoclonal/genetics ; Antibodies, Monoclonal/pharmacology ; Antibody Affinity ; CHO Cells ; Chemical Phenomena ; Cricetulus ; Drug Design ; Drug Evaluation, Preclinical ; Humans ; Neural Cell Adhesion Molecule L1/antagonists & inhibitors ; Protein Stability ; Thermodynamics
Czasopismo naukowe
Tytuł :
Engineering of a Peptide α-N-Methyltransferase to Methylate Non-Proteinogenic Amino Acids.
Autorzy :
Song H; Division of Structural Biology, Wellcome Centre for Human Genetics, Roosevelt Drive, Oxford, OX3 7BN, UK.; The Research Complex at Harwell, Harwell Campus, Oxford, OX11 0FA, UK.; The Rosalind Franklin Institute, Harwell Campus, Oxford, OX11 0FA, UK.
Burton AJ; Department of Chemistry, Frick Chemistry Laboratory, Princeton University, Princeton, NJ, USA.
Shirran SL; Biomedical Sciences Research Complex, North Haugh, University of St. Andrews, Fife, KY16 9ST, UK.
Fahrig-Kamarauskaitė J; Department of Biology, Institute of Microbiology, Eidgenössische Technische Hochschule (ETH) Zürich, Zürich, Switzerland.
Kaspar H; Department of Biology, Institute of Microbiology, Eidgenössische Technische Hochschule (ETH) Zürich, Zürich, Switzerland.
Muir TW; Department of Chemistry, Frick Chemistry Laboratory, Princeton University, Princeton, NJ, USA.
Künzler M; Department of Biology, Institute of Microbiology, Eidgenössische Technische Hochschule (ETH) Zürich, Zürich, Switzerland.
Naismith JH; Division of Structural Biology, Wellcome Centre for Human Genetics, Roosevelt Drive, Oxford, OX3 7BN, UK.; The Research Complex at Harwell, Harwell Campus, Oxford, OX11 0FA, UK.; The Rosalind Franklin Institute, Harwell Campus, Oxford, OX11 0FA, UK.
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Źródło :
Angewandte Chemie (International ed. in English) [Angew Chem Int Ed Engl] 2021 Jun 21; Vol. 60 (26), pp. 14319-14323. Date of Electronic Publication: 2021 May 17.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Protein Engineering*
Amino Acids/*metabolism
Methyltransferases/*metabolism
Peptides/*metabolism
Amides/chemistry ; Amides/metabolism ; Amino Acids/chemistry ; Methylation ; Methyltransferases/chemistry ; Peptides/chemistry ; Protein Conformation
Czasopismo naukowe
Tytuł :
Engineering the protein dynamics of an ancestral luciferase.
Autorzy :
Schenkmayerova A; International Clinical Research Center, St. Anne's University Hospital Brno, Brno, Czech Republic.; Loschmidt Laboratories, Department of Experimental Biology and RECETOX, Faculty of Science, Masaryk University, Brno, Czech Republic.
Pinto GP; International Clinical Research Center, St. Anne's University Hospital Brno, Brno, Czech Republic.; Loschmidt Laboratories, Department of Experimental Biology and RECETOX, Faculty of Science, Masaryk University, Brno, Czech Republic.
Toul M; International Clinical Research Center, St. Anne's University Hospital Brno, Brno, Czech Republic.; Loschmidt Laboratories, Department of Experimental Biology and RECETOX, Faculty of Science, Masaryk University, Brno, Czech Republic.
Marek M; Loschmidt Laboratories, Department of Experimental Biology and RECETOX, Faculty of Science, Masaryk University, Brno, Czech Republic.
Hernychova L; Research Centre for Applied Molecular Oncology, Masaryk Memorial Cancer Institute, Brno, Czech Republic.
Planas-Iglesias J; International Clinical Research Center, St. Anne's University Hospital Brno, Brno, Czech Republic.; Loschmidt Laboratories, Department of Experimental Biology and RECETOX, Faculty of Science, Masaryk University, Brno, Czech Republic.
Daniel Liskova V; International Clinical Research Center, St. Anne's University Hospital Brno, Brno, Czech Republic.; Loschmidt Laboratories, Department of Experimental Biology and RECETOX, Faculty of Science, Masaryk University, Brno, Czech Republic.
Pluskal D; Loschmidt Laboratories, Department of Experimental Biology and RECETOX, Faculty of Science, Masaryk University, Brno, Czech Republic.
Vasina M; International Clinical Research Center, St. Anne's University Hospital Brno, Brno, Czech Republic.; Loschmidt Laboratories, Department of Experimental Biology and RECETOX, Faculty of Science, Masaryk University, Brno, Czech Republic.
Emond S; Department of Biochemistry, University of Cambridge, Cambridge, UK.
Dörr M; Department of Biotechnology and Enzyme Catalysis, Institute of Biochemistry, University of Greifswald, Greifswald, Germany.
Chaloupkova R; Loschmidt Laboratories, Department of Experimental Biology and RECETOX, Faculty of Science, Masaryk University, Brno, Czech Republic.
Bednar D; International Clinical Research Center, St. Anne's University Hospital Brno, Brno, Czech Republic.; Loschmidt Laboratories, Department of Experimental Biology and RECETOX, Faculty of Science, Masaryk University, Brno, Czech Republic.
Prokop Z; International Clinical Research Center, St. Anne's University Hospital Brno, Brno, Czech Republic.; Loschmidt Laboratories, Department of Experimental Biology and RECETOX, Faculty of Science, Masaryk University, Brno, Czech Republic.
Hollfelder F; Department of Biochemistry, University of Cambridge, Cambridge, UK. .
Bornscheuer UT; Department of Biotechnology and Enzyme Catalysis, Institute of Biochemistry, University of Greifswald, Greifswald, Germany. .
Damborsky J; International Clinical Research Center, St. Anne's University Hospital Brno, Brno, Czech Republic. .; Loschmidt Laboratories, Department of Experimental Biology and RECETOX, Faculty of Science, Masaryk University, Brno, Czech Republic. .
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Źródło :
Nature communications [Nat Commun] 2021 Jun 14; Vol. 12 (1), pp. 3616. Date of Electronic Publication: 2021 Jun 14.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Molecular Dynamics Simulation*
Protein Engineering*
Luciferases/*chemistry
Luciferases/*genetics
Luciferases/*metabolism
Animals ; Binding Sites ; Catalysis ; Enzyme Stability ; Kinetics ; Luciferases, Renilla/chemistry ; Luciferases, Renilla/genetics ; Luciferases, Renilla/metabolism ; Mammals ; Mice ; Mutagenesis ; Mutation ; NIH 3T3 Cells ; Protein Conformation ; Temperature
Czasopismo naukowe
Tytuł :
A novel strategy for engineering of a smart generation of immune ribonucleases against EGFR cells.
Autorzy :
Taghizadegan N; Department of Biochemistry, Shahrood Branch, Islamic Azad University, Shahrood, Iran.
Firoozrai M; Department of Biochemistry, Shahrood Branch, Islamic Azad University, Shahrood, Iran.
Nassiri M; Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran.
Ariannejad H; Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran.
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Źródło :
Journal of cellular physiology [J Cell Physiol] 2021 Jun; Vol. 236 (6), pp. 4303-4312. Date of Electronic Publication: 2021 Jan 09.
Typ publikacji :
Journal Article
MeSH Terms :
Protein Engineering*
Amphibian Proteins/*pharmacology
Antineoplastic Agents, Immunological/*pharmacology
Immunotoxins/*pharmacology
Ribonucleases/*pharmacology
Single-Chain Antibodies/*pharmacology
Skin Neoplasms/*drug therapy
Amphibian Proteins/genetics ; Amphibian Proteins/metabolism ; Animals ; Antineoplastic Agents, Immunological/metabolism ; Apoptosis/drug effects ; Binding Sites, Antibody ; CHO Cells ; Cell Line, Tumor ; Cricetulus ; ErbB Receptors/antagonists & inhibitors ; ErbB Receptors/immunology ; ErbB Receptors/metabolism ; HEK293 Cells ; Humans ; Immunotoxins/genetics ; Immunotoxins/metabolism ; Molecular Docking Simulation ; Molecular Dynamics Simulation ; Protein Binding ; Rana pipiens ; Ribonucleases/genetics ; Ribonucleases/metabolism ; Single-Chain Antibodies/metabolism ; Skin Neoplasms/immunology ; Skin Neoplasms/pathology
Czasopismo naukowe
Tytuł :
Enzymes in biotechnology: Critical platform technologies for bioprocess development.
Autorzy :
Katsimpouras C; Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, 02139 MA, USA.
Stephanopoulos G; Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, 02139 MA, USA. Electronic address: .
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Źródło :
Current opinion in biotechnology [Curr Opin Biotechnol] 2021 Jun; Vol. 69, pp. 91-102. Date of Electronic Publication: 2021 Jan 07.
Typ publikacji :
Journal Article; Review
MeSH Terms :
Enzymes, Immobilized*
Protein Engineering*
Biotechnology ; Catalysis ; Cell-Free System ; Enzymes/genetics
Czasopismo naukowe
Tytuł :
Increasing Long-Chain Dicarboxylic Acid Production in Candida tropicalis by Engineering Fatty Transporters.
Autorzy :
Zhang L; State Key Laboratory of Biobased Material and Green Papermaking (LBMP), Qilu University of Technology, Jinan, 250353, Shandong, People's Republic of China.
Xiu X; School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu, 214122, People's Republic of China.
Wang Z; State Key Laboratory of Biobased Material and Green Papermaking (LBMP), Qilu University of Technology, Jinan, 250353, Shandong, People's Republic of China.
Jiang Y; State Key Laboratory of Biobased Material and Green Papermaking (LBMP), Qilu University of Technology, Jinan, 250353, Shandong, People's Republic of China.
Fan H; State Key Laboratory of Biobased Material and Green Papermaking (LBMP), Qilu University of Technology, Jinan, 250353, Shandong, People's Republic of China.
Su J; State Key Laboratory of Biobased Material and Green Papermaking (LBMP), Qilu University of Technology, Jinan, 250353, Shandong, People's Republic of China.
Sui S; Zhucheng Dongxiao Biotechnology Co., Ltd, Xinxing Town, Zhucheng, Shandong, 262200, People's Republic of China.
Wang S; Zhucheng Dongxiao Biotechnology Co., Ltd, Xinxing Town, Zhucheng, Shandong, 262200, People's Republic of China.
Wang R; State Key Laboratory of Biobased Material and Green Papermaking (LBMP), Qilu University of Technology, Jinan, 250353, Shandong, People's Republic of China.
Li J; Zhucheng Dongxiao Biotechnology Co., Ltd, Xinxing Town, Zhucheng, Shandong, 262200, People's Republic of China.
Wang J; State Key Laboratory of Biobased Material and Green Papermaking (LBMP), Qilu University of Technology, Jinan, 250353, Shandong, People's Republic of China. .
Li N; Collage of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, People's Republic of China. .
Wang J; Zhucheng Dongxiao Biotechnology Co., Ltd, Xinxing Town, Zhucheng, Shandong, 262200, People's Republic of China.
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Źródło :
Molecular biotechnology [Mol Biotechnol] 2021 Jun; Vol. 63 (6), pp. 544-555. Date of Electronic Publication: 2021 Mar 30.
Typ publikacji :
Journal Article
MeSH Terms :
Protein Engineering*/methods
Alkanes/*chemistry
Candida tropicalis/*chemistry
Fatty Acids/*chemistry
Alkanes/metabolism ; Amino Acid Sequence/genetics ; Candida tropicalis/enzymology ; Candida tropicalis/metabolism ; Fatty Acid Transport Proteins/metabolism ; Fatty Acids/metabolism ; Fermentation ; Metabolic Networks and Pathways/genetics ; Oxidation-Reduction ; Peroxisomes/enzymology ; Peroxisomes/genetics ; Sequence Alignment
Czasopismo naukowe

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