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Wyszukujesz frazę ""Real-Time Polymerase Chain Reaction"" wg kryterium: Temat


Tytuł :
Development of SYBR Green I-based real-time reverse transcription polymerase chain reaction for the detection of feline astrovirus.
Autorzy :
Wang Y; Anhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, PR China.
Fu Z; Anhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, PR China.
Guo X; Anhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, PR China.
Zhang D; Anhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, PR China.
Bai C; Anhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, PR China.
Li W; Anhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, PR China.
Liu G; Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China.
Li Y; Municipal Key Laboratory of Virology, Ningbo Municipal Center for Disease Control and Prevention, Ningbo 315010, PR China.
Jiang S; Anhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, PR China. Electronic address: .
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Źródło :
Journal of virological methods [J Virol Methods] 2021 Feb; Vol. 288, pp. 114012. Date of Electronic Publication: 2020 Nov 04.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Organic Chemicals*
Real-Time Polymerase Chain Reaction*/methods
Real-Time Polymerase Chain Reaction*/standards
Astroviridae/*genetics
Cat Diseases/*diagnosis
Cat Diseases/*virology
Animals ; Cats ; Reproducibility of Results ; Sensitivity and Specificity
Czasopismo naukowe
Tytuł :
Implication of SARS-CoV-2 evolution in the sensitivity of RT-qPCR diagnostic assays.
Autorzy :
Osório NS; Life and Health Sciences Research Institute, School of Medicine, University of Minho, Braga 4710-057, Portugal. Electronic address: .
Correia-Neves M; Life and Health Sciences Research Institute, School of Medicine, University of Minho, Braga 4710-057, Portugal.
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Źródło :
The Lancet. Infectious diseases [Lancet Infect Dis] 2021 Feb; Vol. 21 (2), pp. 166-167. Date of Electronic Publication: 2020 May 28.
Typ publikacji :
Letter
MeSH Terms :
Evolution, Molecular*
Real-Time Polymerase Chain Reaction*/methods
Real-Time Polymerase Chain Reaction*/standards
COVID-19/*diagnosis
COVID-19/*virology
SARS-CoV-2/*genetics
Genome, Viral ; Humans ; Molecular Diagnostic Techniques/methods ; Molecular Diagnostic Techniques/standards ; RNA, Viral ; Sensitivity and Specificity
Opinia redakcyjna
Tytuł :
Use of Real-Time PCR for Chlamydia psittaci Detection in Human Specimens During an Outbreak of Psittacosis - Georgia and Virginia, 2018.
Autorzy :
McGovern OL
Kobayashi M
Shaw KA
Szablewski C
Gabel J
Holsinger C
Drenzek C
Brennan S
Milucky J
Farrar JL
Wolff BJ
Benitez AJ
Thurman KA
Diaz MH
Winchell JM
Schrag S
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Źródło :
MMWR. Morbidity and mortality weekly report [MMWR Morb Mortal Wkly Rep] 2021 Apr 09; Vol. 70 (14), pp. 505-509. Date of Electronic Publication: 2021 Apr 09.
Typ publikacji :
Journal Article
MeSH Terms :
Disease Outbreaks*
Real-Time Polymerase Chain Reaction*
Chlamydophila psittaci/*isolation & purification
Mass Screening/*methods
Psittacosis/*diagnosis
Adult ; Chlamydophila psittaci/genetics ; Feces/microbiology ; Female ; Georgia/epidemiology ; Humans ; Male ; Middle Aged ; Psittacosis/epidemiology ; Sputum/microbiology ; Virginia/epidemiology ; Young Adult
Czasopismo naukowe
Tytuł :
Development of TaqMan-based real-time RT-PCR assay based on N gene for the quantitative detection of feline morbillivirus.
Autorzy :
Makhtar ST; Faculty of Veterinary Medicine, Universiti Putra Malaysia, UPM Serdang, Selangor, Malaysia.
Tan SW; Institute of Bioscience, Universiti Putra Malaysia, UPM Serdang, Selangor, Malaysia.
Nasruddin NA; Faculty of Veterinary Medicine, Universiti Putra Malaysia, UPM Serdang, Selangor, Malaysia.
Abdul Aziz NA; Faculty of Veterinary Medicine, Universiti Putra Malaysia, UPM Serdang, Selangor, Malaysia.
Omar AR; Faculty of Veterinary Medicine, Universiti Putra Malaysia, UPM Serdang, Selangor, Malaysia.; Institute of Bioscience, Universiti Putra Malaysia, UPM Serdang, Selangor, Malaysia.
Mustaffa-Kamal F; Faculty of Veterinary Medicine, Universiti Putra Malaysia, UPM Serdang, Selangor, Malaysia. .; Institute of Bioscience, Universiti Putra Malaysia, UPM Serdang, Selangor, Malaysia. .
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Źródło :
BMC veterinary research [BMC Vet Res] 2021 Mar 23; Vol. 17 (1), pp. 128. Date of Electronic Publication: 2021 Mar 23.
Typ publikacji :
Journal Article
MeSH Terms :
Morbillivirus/*isolation & purification
Morbillivirus Infections/*veterinary
Real-Time Polymerase Chain Reaction/*veterinary
Animals ; Cross Reactions ; Morbillivirus/genetics ; Morbillivirus Infections/diagnosis ; Real-Time Polymerase Chain Reaction/methods ; Sensitivity and Specificity
SCR Organism :
Feline morbillivirus
Czasopismo naukowe
Tytuł :
Real-time PCR assay as a simple and efficient tool for viral stability study.
Autorzy :
Hu B; Global Vaccines Technical Operations, Merck Manufacturing Division, Merck & Co. Inc., West Point, PA 19486, USA.
Dopp JL; Chemical & Biological Engineering, Iowa State University, Ames, IA 50011, USA.
Salituro GM; Bioanalytics of Pharmacokinetics Pharmacodynamics & Drug Metabolism, Merck & Co. Inc., West Point, PA 19486, USA.
Rubinstein LJ; Bioanalytics of Pharmacokinetics Pharmacodynamics & Drug Metabolism, Merck & Co. Inc., West Point, PA 19486, USA.
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Źródło :
Bioanalysis [Bioanalysis] 2021 Mar; Vol. 13 (5), pp. 387-394. Date of Electronic Publication: 2021 Mar 04.
Typ publikacji :
Journal Article
MeSH Terms :
Real-Time Polymerase Chain Reaction*
RNA, Viral/*metabolism
Virus Diseases/*diagnosis
COVID-19/diagnosis ; COVID-19/virology ; Detergents/chemistry ; Humans ; RNA Stability ; RNA, Viral/blood ; RNA, Viral/urine ; SARS-CoV-2/genetics ; SARS-CoV-2/isolation & purification ; Temperature ; Virus Diseases/virology
Czasopismo naukowe
Tytuł :
Simultaneous and accurate visual identification of chicken, duck and pork components with the molecular amplification integrated lateral flow strip.
Autorzy :
Qin P; Engineering Research Center of Bio-process, MOE, School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, PR China; Research Center for Biomedical and Health Science, School of Life and Health, Anhui Science & Technology University, Fengyang 233100, PR China.
Xu J; Engineering Research Center of Bio-process, MOE, School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, PR China.
Yao L; Engineering Research Center of Bio-process, MOE, School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, PR China.
Wu Q; Engineering Research Center of Bio-process, MOE, School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, PR China.
Yan C; Engineering Research Center of Bio-process, MOE, School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, PR China; Research Center for Biomedical and Health Science, School of Life and Health, Anhui Science & Technology University, Fengyang 233100, PR China.
Lu J; Engineering Research Center of Bio-process, MOE, School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, PR China. Electronic address: .
Yao B; Engineering Research Center of Bio-process, MOE, School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, PR China; Anhui Province Institute of Product Quality Supervision & Inspection, Hefei 230051, PR China.
Liu G; Research Center for Biomedical and Health Science, School of Life and Health, Anhui Science & Technology University, Fengyang 233100, PR China.
Chen W; Engineering Research Center of Bio-process, MOE, School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, PR China. Electronic address: .
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Źródło :
Food chemistry [Food Chem] 2021 Mar 01; Vol. 339, pp. 127891. Date of Electronic Publication: 2020 Aug 20.
Typ publikacji :
Journal Article
MeSH Terms :
Chickens/*genetics
Ducks/*genetics
Meat/*analysis
Real-Time Polymerase Chain Reaction/*methods
Animals ; Cattle ; DNA/isolation & purification ; DNA/metabolism ; Gold/chemistry ; Limit of Detection ; Metal Nanoparticles/chemistry ; Real-Time Polymerase Chain Reaction/instrumentation ; Swine
Czasopismo naukowe
Tytuł :
Real-time PCR assay for Colletotrichum acutatum sensu stricto quantification in olive fruit samples.
Autorzy :
Azevedo-Nogueira F; BioISI - Biosystems & Integrative Sciences Institute, Faculty of Sciences, University of Lisboa, Portugal; University of Trás-os-Montes and Alto Douro, School of Life Science and Environment, Vila Real, Portugal.
Gomes S; BioISI - Biosystems & Integrative Sciences Institute, Faculty of Sciences, University of Lisboa, Portugal; University of Trás-os-Montes and Alto Douro, School of Life Science and Environment, Vila Real, Portugal. Electronic address: .
Lino A; University of Trás-os-Montes and Alto Douro, School of Life Science and Environment, Vila Real, Portugal.
Carvalho T; National Institute for Agricultural and Veterinary Research (INIAV), P.O. Box 6, 7350-951 Elvas, Portugal. Electronic address: .
Martins-Lopes P; BioISI - Biosystems & Integrative Sciences Institute, Faculty of Sciences, University of Lisboa, Portugal; University of Trás-os-Montes and Alto Douro, School of Life Science and Environment, Vila Real, Portugal. Electronic address: .
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Źródło :
Food chemistry [Food Chem] 2021 Mar 01; Vol. 339, pp. 127858. Date of Electronic Publication: 2020 Aug 17.
Typ publikacji :
Journal Article
MeSH Terms :
Real-Time Polymerase Chain Reaction*
Colletotrichum/*genetics
Colletotrichum/*isolation & purification
Fruit/*microbiology
Olea/*microbiology
Plant Diseases/microbiology ; Species Specificity
Czasopismo naukowe
Tytuł :
High-quality RT-PCR with chemically modified RNA controls.
Autorzy :
Luo G; Key Laboratory of Bio-Resource and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan, 610064, PR China; Department of Clinical Laboratory, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan, China.
Zhang J; Key Laboratory of Bio-Resource and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan, 610064, PR China.
Zhang S; Key Laboratory of Bio-Resource and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan, 610064, PR China.
Hu B; Key Laboratory of Bio-Resource and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan, 610064, PR China.
Hu L; SeNA Research Institute, Atlanta, GA, 30303, USA.
Huang Z; Key Laboratory of Bio-Resource and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan, 610064, PR China; SeNA Research Institute, Atlanta, GA, 30303, USA. Electronic address: .
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Źródło :
Talanta [Talanta] 2021 Mar 01; Vol. 224, pp. 121850. Date of Electronic Publication: 2020 Nov 11.
Typ publikacji :
Journal Article
MeSH Terms :
COVID-19/*diagnosis
COVID-19 Nucleic Acid Testing/*methods
RNA, Viral/*analysis
Real-Time Polymerase Chain Reaction/*methods
Reverse Transcriptase Polymerase Chain Reaction/*methods
COVID-19 Nucleic Acid Testing/instrumentation ; DNA, Viral/analysis ; False Negative Reactions ; Humans ; RNA Stability ; RNA, Viral/chemistry ; Reagent Kits, Diagnostic ; Real-Time Polymerase Chain Reaction/instrumentation ; Reverse Transcriptase Polymerase Chain Reaction/instrumentation ; SARS-CoV-2/genetics
Czasopismo naukowe
Tytuł :
Reverse Transcription Recombinase-Aided Amplification Assay With Lateral Flow Dipstick Assay for Rapid Detection of 2019 Novel Coronavirus.
Autorzy :
Zheng YZ; School of Food Engineering and Biotechnology, Hanshan Normal University, Chaozhou, China.
Chen JT; Department of Medical Laboratory, Huizhou Central People's Hospital, Huizhou, China.
Li J; Department of Human Parasitology, School of Basic Medical Sciences, Hubei University of Medicine, Shiyan, China.
Wu XJ; Department of Medical Laboratory, Huizhou Central People's Hospital, Huizhou, China.
Wen JZ; Department of Medical Laboratory, Center for Disease Control and Prevention, Chaozhou, China.
Liu XZ; Department of Medical Laboratory, Chaozhou People's Hospital, Shantou University Medical College, Chaozhou, China.
Lin LY; School of Food Engineering and Biotechnology, Hanshan Normal University, Chaozhou, China.
Liang XY; Department of Medical Laboratory, Huizhou Central People's Hospital, Huizhou, China.; Department of Medical Laboratory, Chaozhou People's Hospital, Shantou University Medical College, Chaozhou, China.
Huang HY; Department of Medical Laboratory, Chaozhou People's Hospital, Shantou University Medical College, Chaozhou, China.
Zha GC; School of Food Engineering and Biotechnology, Hanshan Normal University, Chaozhou, China.
Yang PK; School of Food Engineering and Biotechnology, Hanshan Normal University, Chaozhou, China.
Li LJ; Department of Research and Development, Chaozhou Hybribio Limited Corporation, Chaozhou, China.
Zhong TY; Department of Laboratory Medicine, First Affiliated Hospital of Gannan Medical University, Ganzhou, China.
Liu L; Department of Human Parasitology, School of Basic Medical Sciences, Hubei University of Medicine, Shiyan, China.
Cheng WJ; Department of Human Parasitology, School of Basic Medical Sciences, Hubei University of Medicine, Shiyan, China.
Song XN; Department of Human Parasitology, School of Basic Medical Sciences, Hubei University of Medicine, Shiyan, China.
Lin M; School of Food Engineering and Biotechnology, Hanshan Normal University, Chaozhou, China.
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Źródło :
Frontiers in cellular and infection microbiology [Front Cell Infect Microbiol] 2021 Feb 01; Vol. 11, pp. 613304. Date of Electronic Publication: 2021 Feb 01 (Print Publication: 2021).
Typ publikacji :
Evaluation Study; Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
COVID-19/*diagnosis
COVID-19 Nucleic Acid Testing/*methods
Real-Time Polymerase Chain Reaction/*methods
SARS-CoV-2/*genetics
COVID-19/virology ; COVID-19 Nucleic Acid Testing/instrumentation ; Coronavirus Nucleocapsid Proteins/genetics ; DNA Primers/genetics ; Humans ; Phosphoproteins/genetics ; Point-of-Care Testing ; RNA, Viral/genetics ; Real-Time Polymerase Chain Reaction/instrumentation ; Recombinases/metabolism ; Reverse Transcription ; SARS-CoV-2/isolation & purification ; Sensitivity and Specificity
Czasopismo naukowe
Tytuł :
Simpler and faster Covid-19 testing: Strategies to streamline SARS-CoV-2 molecular assays.
Autorzy :
Panpradist N; Department of Bioengineering, University of Washington, Seattle, WA, United States; Global Health of Women, Adolescents, and Children (Global WACh), School of Public Health, University of Washington, Seattle, WA, United States.
Wang Q; Department of Bioengineering, University of Washington, Seattle, WA, United States.
Ruth PS; Department of Bioengineering, University of Washington, Seattle, WA, United States; Paul G. Allen School of Computer Science & Engineering, University of Washington, Seattle, WA, United States.
Kotnik JH; Department of Bioengineering, University of Washington, Seattle, WA, United States; Department of Family Medicine, University of Washington, Seattle, WA, United States.
Oreskovic AK; Department of Bioengineering, University of Washington, Seattle, WA, United States.
Miller A; Department of Bioengineering, University of Washington, Seattle, WA, United States.
Stewart SWA; Department of Bioengineering, University of Washington, Seattle, WA, United States; Center for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, WA, United States.
Vrana J; Department of Bioengineering, University of Washington, Seattle, WA, United States.
Han PD; Department of Genome Sciences, Seattle, WA, United States; Brotman Baty Institute for Precision Medicine, Seattle, WA, United States.
Beck IA; Center for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, WA, United States.
Starita LM; Department of Genome Sciences, Seattle, WA, United States; Brotman Baty Institute for Precision Medicine, Seattle, WA, United States.
Frenkel LM; Center for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, WA, United States; Departments of Global Health, Medicine, Paediatrics, and Laboratory Medicine, University of Washington, Seattle, WA, United States. Electronic address: .
Lutz BR; Department of Bioengineering, University of Washington, Seattle, WA, United States; Brotman Baty Institute for Precision Medicine, Seattle, WA, United States. Electronic address: .
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Źródło :
EBioMedicine [EBioMedicine] 2021 Feb; Vol. 64, pp. 103236. Date of Electronic Publication: 2021 Feb 12.
Typ publikacji :
Clinical Trial; Journal Article; Multicenter Study
MeSH Terms :
COVID-19*/diagnosis
COVID-19*/genetics
COVID-19 Nucleic Acid Testing*
Real-Time Polymerase Chain Reaction*
Reverse Transcriptase Polymerase Chain Reaction*
RNA, Viral/*genetics
SARS-CoV-2/*genetics
Humans ; Sensitivity and Specificity
Czasopismo naukowe
Tytuł :
Comparison and correlation of commercial SARS-CoV-2 real-time-PCR assays, Ireland, June 2020.
Autorzy :
Carroll A; Public Health Laboratory-Health Service Executive-Dublin, Ireland.
McNamara E; Public Health Laboratory-Health Service Executive-Dublin, Ireland.
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Źródło :
Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin [Euro Surveill] 2021 Feb; Vol. 26 (6).
Typ publikacji :
Comparative Study; Journal Article
MeSH Terms :
Reagent Kits, Diagnostic*
Real-Time Polymerase Chain Reaction*
SARS-CoV-2/*isolation & purification
COVID-19/diagnosis ; Humans ; Ireland ; Reproducibility of Results ; SARS-CoV-2/genetics
Czasopismo naukowe
Tytuł :
Identification of MYCN -Related Gene as a Potential Biomarker for Neuroblastoma Prognostic Model by Integrated Analysis and Quantitative Real-Time PCR.
Autorzy :
Shao F; Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Pediatrics, Department of Pediatric Surgical Oncology, National Clinical Research Center for Child Health and Disorders, China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Children's Hospital of Chongqing Medical University, Chongqing, P.R. China.
Wang Z; Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Pediatrics, Department of Pediatric Surgical Oncology, National Clinical Research Center for Child Health and Disorders, China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Children's Hospital of Chongqing Medical University, Chongqing, P.R. China.
Wang S; Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Pediatrics, Department of Pediatric Surgical Oncology, National Clinical Research Center for Child Health and Disorders, China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Children's Hospital of Chongqing Medical University, Chongqing, P.R. China.
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Źródło :
DNA and cell biology [DNA Cell Biol] 2021 Feb; Vol. 40 (2), pp. 332-347. Date of Electronic Publication: 2020 Dec 30.
Typ publikacji :
Journal Article
MeSH Terms :
Computational Biology*
Real-Time Polymerase Chain Reaction*
Biomarkers, Tumor/*genetics
N-Myc Proto-Oncogene Protein/*genetics
Neuroblastoma/*diagnosis
Neuroblastoma/*genetics
20-Hydroxysteroid Dehydrogenases/genetics ; Adaptor Proteins, Signal Transducing/genetics ; Cyclic AMP-Dependent Protein Kinase Catalytic Subunits/genetics ; Cytoskeletal Proteins/genetics ; DNA Helicases/genetics ; Gene Expression Regulation, Neoplastic ; Humans ; Nerve Tissue Proteins/genetics ; Prognosis
Czasopismo naukowe
Tytuł :
Comparison of three TaqMan real-time reverse transcription-PCR assays in detecting SARS-CoV-2.
Autorzy :
Xiao Y; National Health Commission Key Laboratory of Systems Biology of Pathogens and Christophe Mérieux Laboratory, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100730, PR China.
Li Z; Key Laboratory of Medical Molecular Cell Biology of Shanxi Province, Institutes of Biomedical Sciences, Shanxi University, Taiyuan, 030006, PR China.
Wang X; National Health Commission Key Laboratory of Systems Biology of Pathogens and Christophe Mérieux Laboratory, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100730, PR China.
Wang Y; National Health Commission Key Laboratory of Systems Biology of Pathogens and Christophe Mérieux Laboratory, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100730, PR China.
Wang Y; National Health Commission Key Laboratory of Systems Biology of Pathogens and Christophe Mérieux Laboratory, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100730, PR China.
Wang G; National Health Commission Key Laboratory of Systems Biology of Pathogens and Christophe Mérieux Laboratory, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100730, PR China.
Ren L; National Health Commission Key Laboratory of Systems Biology of Pathogens and Christophe Mérieux Laboratory, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100730, PR China; Key Laboratory of Respiratory Disease Pathogenomics, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100730, PR China. Electronic address: .
Li J; Key Laboratory of Medical Molecular Cell Biology of Shanxi Province, Institutes of Biomedical Sciences, Shanxi University, Taiyuan, 030006, PR China; The Provincial Key Laboratories for Prevention and Treatment of Major Infectious Diseases, Shanxi, Taiyuan, 030006, PR China. Electronic address: .
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Źródło :
Journal of virological methods [J Virol Methods] 2021 Feb; Vol. 288, pp. 114030. Date of Electronic Publication: 2020 Dec 01.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Real-Time Polymerase Chain Reaction*/methods
Reverse Transcriptase Polymerase Chain Reaction*/methods
COVID-19/*diagnosis
COVID-19/*virology
COVID-19 Testing/*methods
SARS-CoV-2/*genetics
COVID-19 Testing/standards ; Humans ; Reproducibility of Results ; Sensitivity and Specificity
Czasopismo naukowe
Tytuł :
Consideration of the Cycle Threshold Values from Real-Time RT-PCR SARS-CoV-2 Interpretation for the Clinicians: Analysis of 339 Positive Cases from a Referral Laboratory in Jakarta, Indonesia.
Autorzy :
Ibrahim F; Clinical Microbiology Department, faculty of medicine, University of Indonesia. .
Natasha A
Saharman YR
Yasmon A
Fithriyah F
Karuniawati A
Ganiesa S
Sudarmono P
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Źródło :
Acta medica Indonesiana [Acta Med Indones] 2021 Jan; Vol. 53 (1), pp. 13-17.
Typ publikacji :
Journal Article
MeSH Terms :
COVID-19*/diagnosis
COVID-19*/epidemiology
COVID-19*/physiopathology
COVID-19 Nucleic Acid Testing*/methods
COVID-19 Nucleic Acid Testing*/standards
COVID-19 Nucleic Acid Testing*/statistics & numerical data
Real-Time Polymerase Chain Reaction*/methods
Real-Time Polymerase Chain Reaction*/statistics & numerical data
Symptom Assessment*/methods
Symptom Assessment*/statistics & numerical data
SARS-CoV-2/*isolation & purification
Adult ; Age Factors ; Correlation of Data ; Female ; Humans ; Indonesia/epidemiology ; Male ; Middle Aged ; Observer Variation ; Reproducibility of Results ; SARS-CoV-2/physiology ; Severity of Illness Index ; Sex Factors ; Viral Load
Czasopismo naukowe
Tytuł :
Analytical comparisons of SARS-COV-2 detection by qRT-PCR and ddPCR with multiple primer/probe sets.
Autorzy :
Liu X; State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, People's Republic of China.
Feng J; State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, People's Republic of China.
Zhang Q; State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, People's Republic of China.
Guo D; State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, People's Republic of China.
Zhang L; State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, People's Republic of China.
Suo T; State Key Laboratory of Virology, Renmin Hospital, Wuhan University, Wuhan, People's Republic of China.
Hu W; Department of Infectious Disease, Zhongnan Hospital, Wuhan University, Wuhan, People's Republic of China.
Guo M; State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, People's Republic of China.
Wang X; State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, People's Republic of China.
Huang Z; State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, People's Republic of China.
Xiong Y; Department of Infectious Disease, Zhongnan Hospital, Wuhan University, Wuhan, People's Republic of China.
Chen G; State Key Laboratory of Virology, Renmin Hospital, Wuhan University, Wuhan, People's Republic of China.
Chen Y; State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, People's Republic of China.
Lan K; State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, People's Republic of China.; Frontier Science Center for Immunology and Metabolism, Wuhan University, Wuhan, People's Republic of China.
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Źródło :
Emerging microbes & infections [Emerg Microbes Infect] 2020 Dec; Vol. 9 (1), pp. 1175-1179.
Typ publikacji :
Letter
MeSH Terms :
Multiplex Polymerase Chain Reaction*/methods
Real-Time Polymerase Chain Reaction*/methods
Real-Time Polymerase Chain Reaction*/standards
Betacoronavirus/*genetics
Coronavirus Infections/*diagnosis
Coronavirus Infections/*virology
Pneumonia, Viral/*diagnosis
Pneumonia, Viral/*virology
COVID-19 ; DNA Primers ; DNA Probes ; Humans ; Pandemics ; SARS-CoV-2 ; Sensitivity and Specificity ; Viral Load
Opinia redakcyjna
Tytuł :
Development of a fully automated high throughput PCR for the detection of SARS-CoV-2: The need for speed.
Autorzy :
Mayer FJ; Divison of Clinical Virology, Department of Laboratory Medicine, Medical University of Vienna , Vienna, Austria.
Ratzinger F; Ihr Labor, Medical Diagnostic Laboratories , Vienna, Austria.
Schmidt RLJ; Divison of Clinical Virology, Department of Laboratory Medicine, Medical University of Vienna , Vienna, Austria.
Greiner G; Divison of Clinical Virology, Department of Laboratory Medicine, Medical University of Vienna , Vienna, Austria.
Landt O; Tib-Molbiol , Berlin, Germany.
Am Ende A; Tib-Molbiol , Berlin, Germany.
Corman VM; Charité - Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF) , Berlin, Germany.
Perkmann-Nagele N; Divison of Clinical Virology, Department of Laboratory Medicine, Medical University of Vienna , Vienna, Austria.
Watkins-Riedel T; Divison of Clinical Virology, Department of Laboratory Medicine, Medical University of Vienna , Vienna, Austria.
Petermann D; Divison of Clinical Virology, Department of Laboratory Medicine, Medical University of Vienna , Vienna, Austria.
Abadir K; QIAGEN GmbH , Vienna, Austria.
Zweimüller-Mayer J; QIAGEN GmbH , Vienna, Austria.
Strassl R; Divison of Clinical Virology, Department of Laboratory Medicine, Medical University of Vienna , Vienna, Austria.
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Źródło :
Virulence [Virulence] 2020 Dec; Vol. 11 (1), pp. 964-967.
Typ publikacji :
Journal Article
MeSH Terms :
Betacoronavirus/*isolation & purification
High-Throughput Nucleotide Sequencing/*methods
RNA, Viral/*isolation & purification
Real-Time Polymerase Chain Reaction/*methods
Betacoronavirus/genetics ; High-Throughput Nucleotide Sequencing/instrumentation ; High-Throughput Nucleotide Sequencing/standards ; Humans ; RNA, Viral/genetics ; Real-Time Polymerase Chain Reaction/instrumentation ; Real-Time Polymerase Chain Reaction/standards ; Reproducibility of Results ; SARS-CoV-2 ; Sensitivity and Specificity ; Time Factors
Czasopismo naukowe
Tytuł :
Evaluation and Comparison of the Hologic Aptima SARS-CoV-2 Assay and the CDC 2019-nCoV Real-Time Reverse Transcription-PCR Diagnostic Panel Using a Four-Sample Pooling Approach.
Autorzy :
Mitchell SL; Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA .
Ventura SE; Clinical Microbiology Laboratory, UPMC Clinical Laboratory Building, Pittsburgh, Pennsylvania, USA.
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Źródło :
Journal of clinical microbiology [J Clin Microbiol] 2020 Nov 18; Vol. 58 (12). Date of Electronic Publication: 2020 Nov 18 (Print Publication: 2020).
Typ publikacji :
Letter
MeSH Terms :
Real-Time Polymerase Chain Reaction*/methods
Real-Time Polymerase Chain Reaction*/standards
COVID-19/*diagnosis
COVID-19/*virology
SARS-CoV-2/*genetics
Humans ; Reproducibility of Results ; Sensitivity and Specificity
Opinia redakcyjna
Tytuł :
An Ultra-Rapid Real-Time RT-PCR Method Using the PCR1100 to Detect Severe Acute Respiratory Syndrome Coronavirus-2.
Autorzy :
Shirato K; Laboratory of Acute Respiratory Viral Diseases and Cytokines, Department of Virology III, National Institute of Infectious Diseases, Japan.
Nao N; Laboratory of Acute Respiratory Viral Diseases and Cytokines, Department of Virology III, National Institute of Infectious Diseases, Japan.
Matsuyama S; Laboratory of Acute Respiratory Viral Diseases and Cytokines, Department of Virology III, National Institute of Infectious Diseases, Japan.
Takeda M; Laboratory of Acute Respiratory Viral Diseases and Cytokines, Department of Virology III, National Institute of Infectious Diseases, Japan.
Kageyama T; Influenza Virus Research Center, National Institute of Infectious Diseases, Japan.
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Źródło :
Japanese journal of infectious diseases [Jpn J Infect Dis] 2021 Jan 22; Vol. 74 (1), pp. 29-34. Date of Electronic Publication: 2020 Jun 30.
Typ publikacji :
Journal Article
MeSH Terms :
COVID-19/*virology
COVID-19 Testing/*instrumentation
Real-Time Polymerase Chain Reaction/*instrumentation
Reverse Transcriptase Polymerase Chain Reaction/*instrumentation
SARS-CoV-2/*isolation & purification
COVID-19 Testing/methods ; Humans ; Real-Time Polymerase Chain Reaction/methods ; Reverse Transcriptase Polymerase Chain Reaction/methods ; SARS-CoV-2/genetics ; Sensitivity and Specificity
Czasopismo naukowe
Tytuł :
Overcoming Supply Shortage for SARS-CoV-2 Detection by RT-qPCR.
Autorzy :
Barra GB; Research and Development Section, Sabin Medicina Diagnóstica, 70632-340 Brasília, Brazil.
Santa Rita TH; Research and Development Section, Sabin Medicina Diagnóstica, 70632-340 Brasília, Brazil.
Mesquita PG; Research and Development Section, Sabin Medicina Diagnóstica, 70632-340 Brasília, Brazil.
Jácomo RH; Research and Development Section, Sabin Medicina Diagnóstica, 70632-340 Brasília, Brazil.
Nery LFA; Research and Development Section, Sabin Medicina Diagnóstica, 70632-340 Brasília, Brazil.
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Źródło :
Genes [Genes (Basel)] 2021 Jan 13; Vol. 12 (1). Date of Electronic Publication: 2021 Jan 13.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
COVID-19*/diagnosis
COVID-19*/genetics
COVID-19 Nucleic Acid Testing*
RNA, Viral*/genetics
RNA, Viral*/metabolism
Real-Time Polymerase Chain Reaction*
Reverse Transcriptase Polymerase Chain Reaction*
SARS-CoV-2*/genetics
SARS-CoV-2*/metabolism
Female ; Humans ; Male
Czasopismo naukowe
Tytuł :
False negative rate of COVID-19 PCR testing: a discordant testing analysis.
Autorzy :
Kanji JN; Public Health Laboratory, Alberta Precision Laboratories, University of Alberta Hospital, 8440 - 112 Street, Edmonton, AB, T6G 2B7, Canada. .; Division of Infectious Diseases, Department of Medicine, University of Alberta, Room 1NW-29, 16940 - 87 Avenue NW, Edmonton, AB, T5R 4H5, Canada. .
Zelyas N; Public Health Laboratory, Alberta Precision Laboratories, University of Alberta Hospital, 8440 - 112 Street, Edmonton, AB, T6G 2B7, Canada.; Department of Laboratory Medicine and Pathology, University of Alberta, University of Alberta Hospital, 8440 - 112 Street, Edmonton, AB, T6G 2B7, Canada.
MacDonald C; Division of Medical Microbiology and Infection Control, Vancouver Coastal Health Vancouver General Hospital, 899 W 12th Avenue, Vancouver, BC, V5Z 1M9, Canada.
Pabbaraju K; Public Health Laboratory, Alberta Precision Laboratories, Foothills Hospital, 1403 - 29 Street NW, Calgary, AB, T2N 2T9, Canada.
Khan MN; Health Protection and Communicable Disease Control, Public Health, Alberta Health Services, Coronation Plaza, 14310 - 111 Avenue NW, Edmonton, AB, T5M 3Z7, Canada.
Prasad A; Health Protection and Communicable Disease Control, Public Health, Alberta Health Services, Coronation Plaza, 14310 - 111 Avenue NW, Edmonton, AB, T5M 3Z7, Canada.
Hu J; Medical Officer of Health (MOH), Public Health, Alberta Health Services, 1213 - 4 Street SW, Calgary, AB, T2R 0X7, Canada.; Department of Community Health Sciences, Cumming School of Medicine, University of Calgary, 1403 - 29 Street NW, Calgary, AB, T2N 2T9, Canada.
Diggle M; Public Health Laboratory, Alberta Precision Laboratories, University of Alberta Hospital, 8440 - 112 Street, Edmonton, AB, T6G 2B7, Canada.; Department of Laboratory Medicine and Pathology, University of Alberta, University of Alberta Hospital, 8440 - 112 Street, Edmonton, AB, T6G 2B7, Canada.
Berenger BM; Public Health Laboratory, Alberta Precision Laboratories, Foothills Hospital, 1403 - 29 Street NW, Calgary, AB, T2N 2T9, Canada.; Department of Pathology and Laboratory Medicine, University of Calgary, 3535 Research Road NW, Calgary, AB, T2L 2K8, Canada.
Tipples G; Public Health Laboratory, Alberta Precision Laboratories, University of Alberta Hospital, 8440 - 112 Street, Edmonton, AB, T6G 2B7, Canada.; Department of Medical Microbiology and Immunology, University of Alberta, 8440 - 112 Street, Edmonton, AB, T6G 2B7, Canada.; Li Ka Shing Institute of Virology, University of Alberta, 6-010 Katz Group Centre for Pharmacy and Research, Edmonton, AB, T6G 2E1, Canada.
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Źródło :
Virology journal [Virol J] 2021 Jan 09; Vol. 18 (1), pp. 13. Date of Electronic Publication: 2021 Jan 09.
Typ publikacji :
Journal Article
MeSH Terms :
COVID-19 Nucleic Acid Testing*/statistics & numerical data
Real-Time Polymerase Chain Reaction*
COVID-19/*diagnosis
SARS-CoV-2/*isolation & purification
Adult ; Aged ; Aged, 80 and over ; COVID-19/virology ; Canada ; False Negative Reactions ; Female ; Humans ; Male ; Middle Aged ; Molecular Diagnostic Techniques/statistics & numerical data ; Sensitivity and Specificity
Czasopismo naukowe

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