Informacja

Drogi użytkowniku, aplikacja do prawidłowego działania wymaga obsługi JavaScript. Proszę włącz obsługę JavaScript w Twojej przeglądarce.

Przeglądasz jako GOŚĆ

Wyszukujesz frazę ""Real-Time Polymerase Chain Reaction"" wg kryterium: Temat


Tytuł :
Innovative Toolbox for the Quantification of Drosophila C Virus, Drosophila A Virus, and Nora Virus.
Autorzy :
Nigg JC; Viruses and RNA Interference Unit, Institut Pasteur, CNRS UMR 3569, 75724 Paris CEDEX 15, France. Electronic address: .
Mongelli V; Viruses and RNA Interference Unit, Institut Pasteur, CNRS UMR 3569, 75724 Paris CEDEX 15, France. Electronic address: .
Blanc H; Viruses and RNA Interference Unit, Institut Pasteur, CNRS UMR 3569, 75724 Paris CEDEX 15, France. Electronic address: .
Saleh MC; Viruses and RNA Interference Unit, Institut Pasteur, CNRS UMR 3569, 75724 Paris CEDEX 15, France. Electronic address: .
Pokaż więcej
Źródło :
Journal of molecular biology [J Mol Biol] 2022 Mar 30; Vol. 434 (6), pp. 167308. Date of Electronic Publication: 2021 Oct 19.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Dicistroviridae*/isolation & purification
Dicistroviridae*/physiology
Drosophila melanogaster*/virology
Immunoassay*
Real-Time Polymerase Chain Reaction*
Animals ; RNA, Viral/genetics ; Virus Replication
SCR Organism :
Drosophila C virus
Czasopismo naukowe
Tytuł :
Development and performance evaluation of TaqMan real-time fluorescence quantitative methylation specific PCR for detecting methylation level of PER2.
Autorzy :
Jiang H; Qingdao University, Qingdao, 266000, Shandong, China.
Yang X; Department of Laboratory Center, Yantai Yuhuangding Hospital Affiliated to Qingdao University, 20 Yuhuangding East Road, Yantai, 264000, Shandong, China.
Mi M; Department of Laboratory Center, Qilu Hospital of Shandong University, Jinan, 250000, Shandong, China.
Wei X; Department of Laboratory Center, Qingdao Women and Children's Hospital, Qingdao, 266000, Shandong, China.
Wu H; Qingdao University, Qingdao, 266000, Shandong, China.
Xin Y; Binzhou Medical University, Yantai, 264000, Shandong, China.
Jiao L; Qingdao University, Qingdao, 266000, Shandong, China.
Sun S; Department of Laboratory Center, Yantaishan Hospital, Yantai, 264000, Shandong, China.
Sun C; Department of Laboratory Center, Yantai Yuhuangding Hospital Affiliated to Qingdao University, 20 Yuhuangding East Road, Yantai, 264000, Shandong, China. .
Pokaż więcej
Źródło :
Molecular biology reports [Mol Biol Rep] 2022 Mar; Vol. 49 (3), pp. 2097-2105. Date of Electronic Publication: 2021 Dec 01.
Typ publikacji :
Journal Article
MeSH Terms :
DNA Methylation*/genetics
Period Circadian Proteins*
Real-Time Polymerase Chain Reaction*/methods
Humans ; Promoter Regions, Genetic/genetics ; Reproducibility of Results ; Sensitivity and Specificity
Czasopismo naukowe
Tytuł :
Application of a Commercial Salmonella Real-Time PCR Assay for the Detection and Quantitation of Salmonella enterica in Poultry Ceca.
Autorzy :
Chaney WE; Diamond V, Cargill Health Technologies, Cedar Rapids, Iowa 52404.
Englishbey AK; Hygiena LLC, Camarillo, California 93012.
Stephens TP; Hygiena LLC, Camarillo, California 93012.
Applegate SF; Department of Animal and Food Sciences, Texas Tech University, Lubbock, Texas 79415, USA.
Sanchez-Plata MX; Department of Animal and Food Sciences, Texas Tech University, Lubbock, Texas 79415, USA.
Pokaż więcej
Źródło :
Journal of food protection [J Food Prot] 2022 Mar 01; Vol. 85 (3), pp. 527-533.
Typ publikacji :
Journal Article
MeSH Terms :
Cecum*/microbiology
Poultry*/microbiology
Real-Time Polymerase Chain Reaction*/methods
Salmonella enterica*/isolation & purification
Animals ; Chickens ; Food Microbiology ; Reproducibility of Results
Czasopismo naukowe
Tytuł :
qPrimerDB: A Powerful and User-Friendly Database for qPCR Primer Design.
Autorzy :
Chang W; College of Agronomy and Biotechnology, Southwest University, Chongqing, China.
Niu Y; College of Agronomy and Biotechnology, Southwest University, Chongqing, China.
Yu M; College of Agronomy and Biotechnology, Southwest University, Chongqing, China.
Li T; State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.; Chongqing Key Laboratory of Microsporidia Infection and Control, Southwest University, Chongqing, China.
Li J; College of Agronomy and Biotechnology, Southwest University, Chongqing, China.; Engineering Research Center of South Upland Agriculture, Ministry of Education, Chongqing, China.; Academy of Agricultural Sciences, Southwest University, Chongqing, China.
Lu K; College of Agronomy and Biotechnology, Southwest University, Chongqing, China. .; Engineering Research Center of South Upland Agriculture, Ministry of Education, Chongqing, China. .; Academy of Agricultural Sciences, Southwest University, Chongqing, China. .
Pokaż więcej
Źródło :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2392, pp. 173-182.
Typ publikacji :
Journal Article
MeSH Terms :
Real-Time Polymerase Chain Reaction*
DNA Primers/genetics ; Databases, Genetic ; Gene Expression ; Thermodynamics
Czasopismo naukowe
Tytuł :
High-throughput nanofluidic real-time PCR to discriminate Pneumococcal Conjugate Vaccine (PCV)-associated serogroups 6, 18, and 22 to serotypes using modified oligonucleotides.
Autorzy :
Downs SL; South African Medical Research Council, Vaccines and Infectious Diseases Analytics Research Unit, School of Pathology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa. .; Department of Science and Technology/National Research Foundation, South African Research Chair Initiative in Vaccine Preventable Diseases, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa. .
Madhi SA; South African Medical Research Council, Vaccines and Infectious Diseases Analytics Research Unit, School of Pathology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa.; Department of Science and Technology/National Research Foundation, South African Research Chair Initiative in Vaccine Preventable Diseases, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa.
Van der Merwe L; South African Medical Research Council, Vaccines and Infectious Diseases Analytics Research Unit, School of Pathology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa.; Department of Science and Technology/National Research Foundation, South African Research Chair Initiative in Vaccine Preventable Diseases, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa.
Nunes MC; South African Medical Research Council, Vaccines and Infectious Diseases Analytics Research Unit, School of Pathology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa.; Department of Science and Technology/National Research Foundation, South African Research Chair Initiative in Vaccine Preventable Diseases, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa.
Olwagen CP; South African Medical Research Council, Vaccines and Infectious Diseases Analytics Research Unit, School of Pathology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa. .; Department of Science and Technology/National Research Foundation, South African Research Chair Initiative in Vaccine Preventable Diseases, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa. .
Pokaż więcej
Źródło :
Scientific reports [Sci Rep] 2021 Dec 09; Vol. 11 (1), pp. 23728. Date of Electronic Publication: 2021 Dec 09.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Real-Time Polymerase Chain Reaction*/methods
Real-Time Polymerase Chain Reaction*/standards
Pneumococcal Vaccines/*genetics
Streptococcus pneumoniae/*classification
Streptococcus pneumoniae/*genetics
Vaccines, Conjugate/*genetics
High-Throughput Screening Assays ; Humans ; Oligonucleotides ; Pneumococcal Infections/diagnosis ; Pneumococcal Infections/immunology ; Pneumococcal Infections/microbiology ; Pneumococcal Infections/prevention & control ; Pneumococcal Vaccines/immunology ; Sensitivity and Specificity ; Serogroup ; Streptococcus pneumoniae/immunology ; Vaccines, Conjugate/immunology
Czasopismo naukowe
Tytuł :
Detection of mecA and 16S rRNA Genes Using Real-Time PCR Can Be Useful in Diagnosing Iliopsoas Abscess, Especially in Culture-Negative Cases: RT-PCR for Iliopsoas Abscess.
Autorzy :
Choe H; Yokohama City University Department of Orthopaedic Surgery, 236-0004 3-9 Fukuura, Kanazawa-ku, Yokohama City, Kanagawa, Japan.
Kobayashi N; Yokohama City University Medical Centre Department of Orthopaedic Surgery, 232-0024 4-57 Urahune-cho, Minami-ku, Yokohama City, Kanagawa, Japan.
Ito Y; Yokohama City University Department of Orthopaedic Surgery, 236-0004 3-9 Fukuura, Kanazawa-ku, Yokohama City, Kanagawa, Japan.
Ike H; Yokohama City University Department of Orthopaedic Surgery, 236-0004 3-9 Fukuura, Kanazawa-ku, Yokohama City, Kanagawa, Japan.
Tezuka T; Yokohama City University Department of Orthopaedic Surgery, 236-0004 3-9 Fukuura, Kanazawa-ku, Yokohama City, Kanagawa, Japan.
Takeyama M; Yokohama City University Department of Orthopaedic Surgery, 236-0004 3-9 Fukuura, Kanazawa-ku, Yokohama City, Kanagawa, Japan.
Kawabata Y; Yokohama City University Department of Orthopaedic Surgery, 236-0004 3-9 Fukuura, Kanazawa-ku, Yokohama City, Kanagawa, Japan.
Inaba Y; Yokohama City University Department of Orthopaedic Surgery, 236-0004 3-9 Fukuura, Kanazawa-ku, Yokohama City, Kanagawa, Japan.
Pokaż więcej
Źródło :
BioMed research international [Biomed Res Int] 2022 Feb 11; Vol. 2022, pp. 2209609. Date of Electronic Publication: 2022 Feb 11 (Print Publication: 2022).
Typ publikacji :
Journal Article
MeSH Terms :
Real-Time Polymerase Chain Reaction*
Psoas Abscess/*diagnosis
RNA, Ribosomal, 16S/*analysis
Adult ; Aged ; Aged, 80 and over ; Biomarkers/analysis ; Female ; Genes, rRNA ; Humans ; Image-Guided Biopsy ; Male ; Middle Aged ; Predictive Value of Tests ; Psoas Abscess/genetics ; Sensitivity and Specificity
Czasopismo naukowe
Tytuł :
SARS-CoV-2 RT-qPCR Test Detection Rates Are Associated with Patient Age, Sex, and Time since Diagnosis.
Autorzy :
Levine-Tiefenbrun M; Biology Faculty, Technion-Israel Institute of Technology, Haifa, Israel.
Yelin I; Biology Faculty, Technion-Israel Institute of Technology, Haifa, Israel. Electronic address: .
Uriel H; Faculty of Computer Science, Technion-Israel Institute of Technology, Haifa, Israel.
Kuint J; Maccabitech, Maccabi Health Services, Tel Aviv, Israel; Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv, Israel.
Schreiber L; Maccabi Megalab, Maccabi Healthcare Services, Rehovot, Israel.
Herzel E; Maccabitech, Maccabi Health Services, Tel Aviv, Israel.
Katz R; Maccabitech, Maccabi Health Services, Tel Aviv, Israel.
Ben-Tov A; Maccabitech, Maccabi Health Services, Tel Aviv, Israel; Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv, Israel.
Gazit S; Maccabitech, Maccabi Health Services, Tel Aviv, Israel.
Patalon T; Maccabitech, Maccabi Health Services, Tel Aviv, Israel.
Chodick G; Maccabitech, Maccabi Health Services, Tel Aviv, Israel; Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv, Israel.
Kishony R; Biology Faculty, Technion-Israel Institute of Technology, Haifa, Israel; Faculty of Computer Science, Technion-Israel Institute of Technology, Haifa, Israel.
Pokaż więcej
Źródło :
The Journal of molecular diagnostics : JMD [J Mol Diagn] 2022 Feb; Vol. 24 (2), pp. 112-119. Date of Electronic Publication: 2021 Nov 23.
Typ publikacji :
Journal Article
MeSH Terms :
COVID-19 Testing*/methods
Real-Time Polymerase Chain Reaction*/methods
COVID-19/*diagnosis
COVID-19/*epidemiology
COVID-19/*virology
SARS-CoV-2/*genetics
Adult ; Age Factors ; Female ; Humans ; Male ; Middle Aged ; Odds Ratio ; RNA, Viral ; Sensitivity and Specificity ; Sex Factors ; Time Factors ; Viral Load ; Young Adult
Czasopismo naukowe
Tytuł :
A Method for Evaluation of the Level of Circulating Mitochondrial DNA by ND1 and ND2 Genes.
Autorzy :
Ogarkov OB; Research Center for Family Health and Human Reproduction Problems, Irkutsk, Russia. .
Orlova EA; Research Center for Family Health and Human Reproduction Problems, Irkutsk, Russia.
Malov IV; Irkutsk State Medical University, Ministry of Health of the Russian Federation, Irkutsk, Russia.
Zhdanova SN; Research Center for Family Health and Human Reproduction Problems, Irkutsk, Russia.
Malov SI; Irkutsk State Medical University, Ministry of Health of the Russian Federation, Irkutsk, Russia.
Khromova PA; Research Center for Family Health and Human Reproduction Problems, Irkutsk, Russia.
Stepanenko LA; Irkutsk State Medical University, Ministry of Health of the Russian Federation, Irkutsk, Russia.
Sinkov VV; Research Center for Family Health and Human Reproduction Problems, Irkutsk, Russia.
Rychkova LV; Research Center for Family Health and Human Reproduction Problems, Irkutsk, Russia.
Kolesnikova LI; Research Center for Family Health and Human Reproduction Problems, Irkutsk, Russia.
Pokaż więcej
Źródło :
Bulletin of experimental biology and medicine [Bull Exp Biol Med] 2022 Feb; Vol. 172 (4), pp. 495-498. Date of Electronic Publication: 2022 Feb 17.
Typ publikacji :
Journal Article
MeSH Terms :
COVID-19/*metabolism
Cell-Free Nucleic Acids/*genetics
DNA, Mitochondrial/*genetics
NADH Dehydrogenase/*genetics
Real-Time Polymerase Chain Reaction/*standards
Adult ; Aged ; COVID-19/virology ; Case-Control Studies ; Cell-Free Nucleic Acids/blood ; DNA Primers/chemical synthesis ; DNA, Mitochondrial/blood ; Female ; Humans ; Male ; Middle Aged ; Mitochondria/genetics ; Mitochondria/virology ; NADH Dehydrogenase/blood ; Real-Time Polymerase Chain Reaction/methods ; SARS-CoV-2/pathogenicity
Czasopismo naukowe
Tytuł :
Log ( Lactobacillus crispatus / Gardnerella vaginalis ): a new indicator of diagnosing bacterial vaginosis.
Autorzy :
Deng T; Department of Laboratory Medicine, Tongde Hospital of Zhejiang Province, Hangzhou, P.R. China.
Shang A; Department of Laboratory Medicine, Shanghai Tongji Hospital, School of Medicine, Tongji University, Shanghai, P.R. China.
Zheng Y; Department of Obstetrics and Gynecology, Tongde Hospital of Zhejiang Province, Hangzhou, P.R. China.
Zhang L; Department of Laboratory Medicine, Tongde Hospital of Zhejiang Province, Hangzhou, P.R. China.
Sun H; Department of Laboratory Medicine, Tongde Hospital of Zhejiang Province, Hangzhou, P.R. China.
Wang W; Department of Laboratory Medicine, Tongde Hospital of Zhejiang Province, Hangzhou, P.R. China.
Pokaż więcej
Źródło :
Bioengineered [Bioengineered] 2022 Feb; Vol. 13 (2), pp. 2981-2991.
Typ publikacji :
Evaluation Study; Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Gardnerella vaginalis/*genetics
Lactobacillus crispatus/*genetics
RNA, Ribosomal, 16S/*analysis
Real-Time Polymerase Chain Reaction/*methods
Vaginosis, Bacterial/*diagnosis
Adolescent ; Adult ; China ; Cohort Studies ; Diagnostic Techniques, Obstetrical and Gynecological ; Female ; Gardnerella vaginalis/isolation & purification ; Humans ; Lactobacillus crispatus/isolation & purification ; Middle Aged ; RNA, Bacterial/analysis ; RNA-Seq ; Real-Time Polymerase Chain Reaction/standards ; Reproducibility of Results ; Sensitivity and Specificity ; Sequence Analysis, RNA/methods ; Vaginosis, Bacterial/microbiology ; Young Adult
Czasopismo naukowe
Tytuł :
SARS-CoV-2 detection in nasopharyngeal swabs: Performance characteristics of a real-time RT-qPCR and a droplet digital RT-PCR assay based on the exonuclease region (ORF1b, nsp 14).
Autorzy :
Pierri B; Centro di Referenza Nazionale per l'analisi e studio di correlazione tra ambiente, animale e uomo, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Portici, NA, Italy; Department of Medicine, Surgery and Dentistry 'Scuola Medica Salernitana', University of Salerno, Baronissi, SA, Italy.
Mancusi A; Department of Food Microbiology, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Portici, NA, Italy.
Proroga YTR; Department of Food Microbiology, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Portici, NA, Italy.
Capuano F; Department of Food Microbiology, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Portici, NA, Italy.
Cerino P; Centro di Referenza Nazionale per l'analisi e studio di correlazione tra ambiente, animale e uomo, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Portici, NA, Italy.
Girardi S; Department of Food Microbiology, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Portici, NA, Italy.
Vassallo L; Centro di Referenza Nazionale per l'analisi e studio di correlazione tra ambiente, animale e uomo, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Portici, NA, Italy.
Lo Conte G; Centro di Referenza Nazionale per l'analisi e studio di correlazione tra ambiente, animale e uomo, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Portici, NA, Italy.
Tafuro M; Centro di Referenza Nazionale per l'analisi e studio di correlazione tra ambiente, animale e uomo, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Portici, NA, Italy.
Cuomo MC; Centro di Referenza Nazionale per l'analisi e studio di correlazione tra ambiente, animale e uomo, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Portici, NA, Italy.
Di Concilio D; Centro di Referenza Nazionale per l'analisi e studio di correlazione tra ambiente, animale e uomo, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Portici, NA, Italy.
Vicenza T; Department of Food Safety, Nutrition and Veterinary Public Health, Istituto Superiore di Sanità, Rome, Italy.
Cozzi L; Department of Food Safety, Nutrition and Veterinary Public Health, Istituto Superiore di Sanità, Rome, Italy.
Di Pasquale S; Department of Food Safety, Nutrition and Veterinary Public Health, Istituto Superiore di Sanità, Rome, Italy.
La Rosa G; Department of Environment and Health, Istituto Superiore di Sanità, Rome, Italy.
Beikpour F; Department of Food Safety, Nutrition and Veterinary Public Health, Istituto Superiore di Sanità, Rome, Italy; Department of Veterinary Medicine, University of Bari 'Aldo Moro', Bari, Italy.
Suffredini E; Department of Food Safety, Nutrition and Veterinary Public Health, Istituto Superiore di Sanità, Rome, Italy. Electronic address: .
Pokaż więcej
Źródło :
Journal of virological methods [J Virol Methods] 2022 Feb; Vol. 300, pp. 114420. Date of Electronic Publication: 2021 Dec 10.
Typ publikacji :
Evaluation Study; Journal Article
MeSH Terms :
COVID-19*/diagnosis
Real-Time Polymerase Chain Reaction*
Reverse Transcriptase Polymerase Chain Reaction*
SARS-CoV-2*/isolation & purification
Exonucleases ; Humans ; Nasopharynx/virology ; RNA, Viral/genetics ; Sensitivity and Specificity
Czasopismo naukowe
Tytuł :
A robust biostatistical method leverages informative but uncertainly determined qPCR data for biomarker detection, early diagnosis, and treatment.
Autorzy :
Zhuang W; Division of Bioinformatics and Biostatistics, National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, Arkansas, United States of America.
Camacho L; Division of Biochemical Toxicology, National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, Arkansas, United States of America.
Silva CS; Division of Biochemical Toxicology, National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, Arkansas, United States of America.
Thomson M; Office of New Drugs, Center for Drug Evaluation and Research, U.S. Food and Drug Administration, Silver Spring, Maryland, United States of America.
Snyder K; Office of New Drugs, Center for Drug Evaluation and Research, U.S. Food and Drug Administration, Silver Spring, Maryland, United States of America.
Pokaż więcej
Źródło :
PloS one [PLoS One] 2022 Jan 31; Vol. 17 (1), pp. e0263070. Date of Electronic Publication: 2022 Jan 31 (Print Publication: 2022).
Typ publikacji :
Journal Article; Research Support, U.S. Gov't, P.H.S.
MeSH Terms :
Early Diagnosis*
Models, Theoretical*
Real-Time Polymerase Chain Reaction*
Biomarkers ; Humans
Czasopismo naukowe
Tytuł :
Performance Characteristics of Real-Time PCRs for African Swine Fever Virus Genome Detection-Comparison of Twelve Kits to an OIE-Recommended Method.
Autorzy :
Pikalo J; Friedrich-Loeffler-Institut, Suedufer 10, 17493 Greifswald-Insel Riems, Germany.
Carrau T; Friedrich-Loeffler-Institut, Suedufer 10, 17493 Greifswald-Insel Riems, Germany.
Deutschmann P; Friedrich-Loeffler-Institut, Suedufer 10, 17493 Greifswald-Insel Riems, Germany.
Fischer M; Friedrich-Loeffler-Institut, Suedufer 10, 17493 Greifswald-Insel Riems, Germany.
Schlottau K; Friedrich-Loeffler-Institut, Suedufer 10, 17493 Greifswald-Insel Riems, Germany.
Beer M; Friedrich-Loeffler-Institut, Suedufer 10, 17493 Greifswald-Insel Riems, Germany.
Blome S; Friedrich-Loeffler-Institut, Suedufer 10, 17493 Greifswald-Insel Riems, Germany.
Pokaż więcej
Źródło :
Viruses [Viruses] 2022 Jan 24; Vol. 14 (2). Date of Electronic Publication: 2022 Jan 24.
Typ publikacji :
Evaluation Study; Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
African Swine Fever/*diagnosis
African Swine Fever/*virology
African Swine Fever Virus/*genetics
African Swine Fever Virus/*isolation & purification
Real-Time Polymerase Chain Reaction/*methods
African Swine Fever Virus/classification ; Animal Husbandry/organization & administration ; Animals ; DNA, Viral/genetics ; Genome, Viral ; Genotype ; Germany ; Reagent Kits, Diagnostic ; Real-Time Polymerase Chain Reaction/instrumentation ; Sensitivity and Specificity ; Swine ; World Health Organization
Czasopismo naukowe
Tytuł :
Comparison of pneumococcal colonization density among healthy children and children with respiratory symptoms using real time PCR (RT-PCR).
Autorzy :
Vidanapathirana G; Faculty of Medicine, University of Peradeniya, Kandy, Sri Lanka.
Angulmaduwa ALSK; Department of Microbiology, Faculty of Medicine, University of Peradeniya, Kandy, Sri Lanka.
Munasinghe TS; Department of Paediatrics, Faculty of Medicine, University of Peradeniya, Kandy, Sri Lanka.
Ekanayake EWMA; Department of Microbiology, Faculty of Medicine, University of Peradeniya, Kandy, Sri Lanka.
Harasgama P; Department of Microbiology, Faculty of Medicine, University of Peradeniya, Kandy, Sri Lanka.
Kudagammana ST; Department of Paediatrics, Faculty of Medicine, University of Peradeniya, Kandy, Sri Lanka.
Dissanayake BN; Department of Microbiology, Faculty of Medicine, University of Peradeniya, Kandy, Sri Lanka.
Liyanapathirana LVC; Department of Microbiology, Faculty of Medicine, University of Peradeniya, Kandy, Sri Lanka. .
Pokaż więcej
Źródło :
BMC microbiology [BMC Microbiol] 2022 Jan 20; Vol. 22 (1), pp. 31. Date of Electronic Publication: 2022 Jan 20.
Typ publikacji :
Comparative Study; Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Bacteriological Techniques/*standards
Hospitalization/*statistics & numerical data
Pneumococcal Infections/*microbiology
Real-Time Polymerase Chain Reaction/*standards
Streptococcus pneumoniae/*genetics
Streptococcus pneumoniae/*growth & development
Child, Preschool ; Cohort Studies ; Healthy Volunteers/statistics & numerical data ; Humans ; Infant ; Nasopharynx/microbiology ; Real-Time Polymerase Chain Reaction/methods ; Respiratory Tract Infections/microbiology ; Streptococcus pneumoniae/isolation & purification
Czasopismo naukowe
Tytuł :
Determining Viability of M. ulcerans by 16S rRNA RT Reverse Transcriptase Real-Time PCR.
Autorzy :
Beissner M; Division of Infectious Diseases and Tropical Medicine, University Hospital, Ludwig-Maximilians-University, Munich, Germany. .
Phillips RO; Kwame Nkrumah University of Science and Technology (KNUST), School of Medical Sciences and Kumasi Centre for Collaborative Research in Tropical Medicine (KCCR), Kumasi, Ghana.
Bretzel G; Division of Infectious Diseases and Tropical Medicine, University Hospital, Ludwig-Maximilians-University, Munich, Germany.
Pokaż więcej
Źródło :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2387, pp. 81-86.
Typ publikacji :
Journal Article
MeSH Terms :
Real-Time Polymerase Chain Reaction*
Gram-Positive Bacteria ; RNA, Ribosomal, 16S/genetics ; RNA-Directed DNA Polymerase ; Reverse Transcriptase Polymerase Chain Reaction
Czasopismo naukowe
Tytuł :
Detection of Rubella Virus by Tri-Primer RT-PCR Assay and Genotyping by Fragment RT-PCR.
Autorzy :
George S; Diagnostic Virology Group, National Institute of Virology, Pune, Maharashtra, India. .
Pokaż więcej
Źródło :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2392, pp. 53-64.
Typ publikacji :
Journal Article
MeSH Terms :
Real-Time Polymerase Chain Reaction*
Reverse Transcriptase Polymerase Chain Reaction*
Rubella virus*/genetics
Genotype ; RNA, Viral/genetics
Czasopismo naukowe
Tytuł :
Development of a one-step RT-qPCR detection assay for the newly described citrus viroid VII.
Autorzy :
Chambers GA; NSW Department of Primary Industries, Elizabeth Macarthur Agricultural Institute, Private Bag 4008, Narellan, NSW, 2567, Australia; Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, GPO Box 267, Brisbane, Queensland, 4001, Australia. Electronic address: .
Geering ADW; Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, GPO Box 267, Brisbane, Queensland, 4001, Australia.
Holford P; Western Sydney University, School of Science, LB 1797, Penrith, NSW, 2752, Australia.
Vidalakis G; Department of Microbiology and Plant Pathology, University of California, Riverside, Riverside, CA, United States.
Donovan NJ; NSW Department of Primary Industries, Elizabeth Macarthur Agricultural Institute, Private Bag 4008, Narellan, NSW, 2567, Australia.
Pokaż więcej
Źródło :
Journal of virological methods [J Virol Methods] 2022 Jan; Vol. 299, pp. 114330. Date of Electronic Publication: 2021 Oct 11.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Citrus*/virology
Plant Viruses*/isolation & purification
Real-Time Polymerase Chain Reaction*/methods
Viroids*/genetics
Viroids*/isolation & purification
Australia ; Sensitivity and Specificity
Czasopismo naukowe
Tytuł :
PCR Primer Design for the Rapidly Evolving SARS-CoV-2 Genome.
Autorzy :
Qu W; iGeneTech Bioscience Co., Ltd, Beijing, China. .; Center of Growth, Metabolism and Aging, Key Laboratory of Bio-Resources and Eco-Environment, College of Life Sciences, Sichuan University, Chengdu, China. .
Li J; Information Center, Academy of Military Medical Science, Beijing, China.
Cai H; Center of Growth, Metabolism and Aging, Key Laboratory of Bio-Resources and Eco-Environment, College of Life Sciences, Sichuan University, Chengdu, China.
Zhao D; Information Center, Academy of Military Medical Science, Beijing, China. .
Pokaż więcej
Źródło :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2392, pp. 185-197.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
COVID-19*/diagnosis
Genome, Viral*
Real-Time Polymerase Chain Reaction*
SARS-CoV-2*/genetics
COVID-19 Testing ; Humans
Czasopismo naukowe
Tytuł :
Culture-Independent Detection of Poliovirus in Stool Samples by Direct RNA Extraction.
Autorzy :
Harrington C; Division of Viral Diseases, Centers for Disease Control and Preventiongrid.416738.f, Atlanta, Georgia, USA.
Sun H; Division of Viral Diseases, Centers for Disease Control and Preventiongrid.416738.f, Atlanta, Georgia, USA.
Jeffries-Miles S; Division of Viral Diseases, Centers for Disease Control and Preventiongrid.416738.f, Atlanta, Georgia, USA.
Gerloff N; Division of Viral Diseases, Centers for Disease Control and Preventiongrid.416738.f, Atlanta, Georgia, USA.
Mandelbaum M; Division of Viral Diseases, Centers for Disease Control and Preventiongrid.416738.f, Atlanta, Georgia, USA.
Pang H; Cherokee Nation Assurance, contracting agency to the Division of Viral Diseases, Arlington, Virginia, USA.
Collins N; IHRC, Inc., contracting agency to the Division of Viral Diseases, Atlanta, Georgia, USA.
Burns CC; Division of Viral Diseases, Centers for Disease Control and Preventiongrid.416738.f, Atlanta, Georgia, USA.
Vega E; Division of Viral Diseases, Centers for Disease Control and Preventiongrid.416738.f, Atlanta, Georgia, USA.
Pokaż więcej
Źródło :
Microbiology spectrum [Microbiol Spectr] 2021 Dec 22; Vol. 9 (3), pp. e0066821. Date of Electronic Publication: 2021 Nov 10.
Typ publikacji :
Evaluation Study; Journal Article
MeSH Terms :
Clinical Laboratory Techniques/*methods
Feces/*chemistry
Poliomyelitis/*virology
Poliovirus/*isolation & purification
RNA, Viral/*genetics
RNA, Viral/*isolation & purification
Real-Time Polymerase Chain Reaction/*methods
Feces/virology ; Humans ; Poliomyelitis/diagnosis ; Poliovirus/genetics ; Prospective Studies ; Real-Time Polymerase Chain Reaction/instrumentation ; Retrospective Studies
Czasopismo naukowe
Tytuł :
Development and comparison of loop-mediated isothermal amplification with quantitative PCR for the specific detection of Saprolegnia spp.
Autorzy :
Ghosh S; Department of Biological Sciences, Bowling Green State University, Bowling Green, Ohio, United States of America.
Straus DL; United States Department of Agriculture, Agricultural Research Service, Harry K. Dupree-Stuttgart National Aquaculture Research Center, Stuttgart, Arkansas, United States of America.
Good C; The Conservation Fund's Freshwater Institute, Shepherdstown, West Virginia, United States of America.
Phuntumart V; Department of Biological Sciences, Bowling Green State University, Bowling Green, Ohio, United States of America.
Pokaż więcej
Źródło :
PloS one [PLoS One] 2021 Dec 13; Vol. 16 (12), pp. e0250808. Date of Electronic Publication: 2021 Dec 13 (Print Publication: 2021).
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Molecular Diagnostic Techniques*
Nucleic Acid Amplification Techniques*
Real-Time Polymerase Chain Reaction*
DNA Primers/*genetics
Electron Transport Complex IV/*genetics
Saprolegnia/*genetics
Saprolegnia/classification
SCR Protocol :
LAMP assay
Czasopismo naukowe
Tytuł :
Alternative tissue fixation for combined histopathological and molecular analysis in a clinically representative setting.
Autorzy :
Meecham A; Research Department of Pathology, UCL Cancer Institute, London, UK.
Miranda E; Research Department of Pathology, UCL Cancer Institute, London, UK.
Morris HT; Institute of Cancer Sciences, University of Glasgow, Glasgow, UK.
Hair J; NHS Research Scotland Greater Glasgow and Clyde Biorepository, Glasgow, UK.
Oien KA; Institute of Cancer Sciences, University of Glasgow, Glasgow, UK.
Gerrard G; Research Department of Pathology, UCL Cancer Institute, London, UK.; Sarah Cannon Molecular Diagnostics, HCA Healthcare UK, London, UK.
Guppy N; Research Department of Pathology, UCL Cancer Institute, London, UK.
Mooney D; Department of Pathology, Queen Elizabeth University Hospital, Glasgow, UK.
Shaw EC; Department of Cellular Pathology, University Hospital Southampton NHS Foundation Trust, Southampton, UK.; Cancer Sciences Unit, University of Southampton, Southampton, UK.
Ashton-Key M; Department of Cellular Pathology, University Hospital Southampton NHS Foundation Trust, Southampton, UK.; Cancer Sciences Unit, University of Southampton, Southampton, UK.
Lees R; Department of Cellular Pathology, University Hospital Southampton NHS Foundation Trust, Southampton, UK.; Cancer Sciences Unit, University of Southampton, Southampton, UK.
Flanagan A; Research Department of Pathology, UCL Cancer Institute, London, UK.; Royal National Orthopaedic Hospital NHS Trust, Stanmore, Middlesex, UK.
Rodriguez-Justo M; Research Department of Pathology, UCL Cancer Institute, London, UK. .; University College Hospitals, London, UK. .
Pokaż więcej
Źródło :
Histochemistry and cell biology [Histochem Cell Biol] 2021 Dec; Vol. 156 (6), pp. 595-607. Date of Electronic Publication: 2021 Dec 14.
Typ publikacji :
Journal Article
MeSH Terms :
Immunohistochemistry*
Real-Time Polymerase Chain Reaction*
Tissue Fixation*
DNA/*genetics
RNA/*genetics
Formaldehyde ; Humans
Czasopismo naukowe

Ta witryna wykorzystuje pliki cookies do przechowywania informacji na Twoim komputerze. Pliki cookies stosujemy w celu świadczenia usług na najwyższym poziomie, w tym w sposób dostosowany do indywidualnych potrzeb. Korzystanie z witryny bez zmiany ustawień dotyczących cookies oznacza, że będą one zamieszczane w Twoim komputerze. W każdym momencie możesz dokonać zmiany ustawień dotyczących cookies