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Wyszukujesz frazę ""Real-time polymerase chain reaction"" wg kryterium: Temat


Tytuł :
qPrimerDB: A Powerful and User-Friendly Database for qPCR Primer Design.
Autorzy :
Chang W; College of Agronomy and Biotechnology, Southwest University, Chongqing, China.
Niu Y; College of Agronomy and Biotechnology, Southwest University, Chongqing, China.
Yu M; College of Agronomy and Biotechnology, Southwest University, Chongqing, China.
Li T; State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.; Chongqing Key Laboratory of Microsporidia Infection and Control, Southwest University, Chongqing, China.
Li J; College of Agronomy and Biotechnology, Southwest University, Chongqing, China.; Engineering Research Center of South Upland Agriculture, Ministry of Education, Chongqing, China.; Academy of Agricultural Sciences, Southwest University, Chongqing, China.
Lu K; College of Agronomy and Biotechnology, Southwest University, Chongqing, China. .; Engineering Research Center of South Upland Agriculture, Ministry of Education, Chongqing, China. .; Academy of Agricultural Sciences, Southwest University, Chongqing, China. .
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Źródło :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2392, pp. 173-182.
Typ publikacji :
Journal Article
MeSH Terms :
Real-Time Polymerase Chain Reaction*
DNA Primers/genetics ; Databases, Genetic ; Gene Expression ; Thermodynamics
Czasopismo naukowe
Tytuł :
Determining Viability of M. ulcerans by 16S rRNA RT Reverse Transcriptase Real-Time PCR.
Autorzy :
Beissner M; Division of Infectious Diseases and Tropical Medicine, University Hospital, Ludwig-Maximilians-University, Munich, Germany. .
Phillips RO; Kwame Nkrumah University of Science and Technology (KNUST), School of Medical Sciences and Kumasi Centre for Collaborative Research in Tropical Medicine (KCCR), Kumasi, Ghana.
Bretzel G; Division of Infectious Diseases and Tropical Medicine, University Hospital, Ludwig-Maximilians-University, Munich, Germany.
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Źródło :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2387, pp. 81-86.
Typ publikacji :
Journal Article
MeSH Terms :
Real-Time Polymerase Chain Reaction*
Gram-Positive Bacteria ; RNA, Ribosomal, 16S/genetics ; RNA-Directed DNA Polymerase ; Reverse Transcriptase Polymerase Chain Reaction
Czasopismo naukowe
Tytuł :
Detection of Rubella Virus by Tri-Primer RT-PCR Assay and Genotyping by Fragment RT-PCR.
Autorzy :
George S; Diagnostic Virology Group, National Institute of Virology, Pune, Maharashtra, India. .
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Źródło :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2392, pp. 53-64.
Typ publikacji :
Journal Article
MeSH Terms :
Real-Time Polymerase Chain Reaction*
Reverse Transcriptase Polymerase Chain Reaction*
Rubella virus*/genetics
Genotype ; RNA, Viral/genetics
Czasopismo naukowe
Tytuł :
PCR Primer Design for the Rapidly Evolving SARS-CoV-2 Genome.
Autorzy :
Qu W; iGeneTech Bioscience Co., Ltd, Beijing, China. .; Center of Growth, Metabolism and Aging, Key Laboratory of Bio-Resources and Eco-Environment, College of Life Sciences, Sichuan University, Chengdu, China. .
Li J; Information Center, Academy of Military Medical Science, Beijing, China.
Cai H; Center of Growth, Metabolism and Aging, Key Laboratory of Bio-Resources and Eco-Environment, College of Life Sciences, Sichuan University, Chengdu, China.
Zhao D; Information Center, Academy of Military Medical Science, Beijing, China. .
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Źródło :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2392, pp. 185-197.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
COVID-19*/diagnosis
Genome, Viral*
Real-Time Polymerase Chain Reaction*
SARS-CoV-2*/genetics
COVID-19 Testing ; Humans
Czasopismo naukowe
Tytuł :
Development and comparison of loop-mediated isothermal amplification with quantitative PCR for the specific detection of Saprolegnia spp.
Autorzy :
Ghosh S; Department of Biological Sciences, Bowling Green State University, Bowling Green, Ohio, United States of America.
Straus DL; United States Department of Agriculture, Agricultural Research Service, Harry K. Dupree-Stuttgart National Aquaculture Research Center, Stuttgart, Arkansas, United States of America.
Good C; The Conservation Fund's Freshwater Institute, Shepherdstown, West Virginia, United States of America.
Phuntumart V; Department of Biological Sciences, Bowling Green State University, Bowling Green, Ohio, United States of America.
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Źródło :
PloS one [PLoS One] 2021 Dec 13; Vol. 16 (12), pp. e0250808. Date of Electronic Publication: 2021 Dec 13 (Print Publication: 2021).
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Molecular Diagnostic Techniques*
Nucleic Acid Amplification Techniques*
Real-Time Polymerase Chain Reaction*
DNA Primers/*genetics
Electron Transport Complex IV/*genetics
Saprolegnia/*genetics
Saprolegnia/classification
SCR Protocol :
LAMP assay
Czasopismo naukowe
Tytuł :
A pentaplex real-time PCR assay for rapid identification of major beta-lactamase genes KPC, NDM, CTX, CMY, and OXA-48 directly from bacteria in blood.
Autorzy :
Hoj TR; Department of Microbiology and Molecular Biology, Brigham Young University, Provo, UT 84602, USA.
McNeely B; Department of Microbiology and Molecular Biology, Brigham Young University, Provo, UT 84602, USA.
Webber K; Department of Microbiology and Molecular Biology, Brigham Young University, Provo, UT 84602, USA.
Welling E; Chemical Engineering Department, Brigham Young University, Provo, UT, USA.
Pitt WG; Chemical Engineering Department, Brigham Young University, Provo, UT, USA.
Ford LC; Infectious Diseases, Intermountain Healthcare, Provo, UT USA.
Robison RA; Department of Microbiology and Molecular Biology, Brigham Young University, Provo, UT 84602, USA.
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Źródło :
Journal of medical microbiology [J Med Microbiol] 2021 Dec; Vol. 70 (12).
Typ publikacji :
Journal Article
MeSH Terms :
Real-Time Polymerase Chain Reaction*
Sepsis*/diagnosis
Sepsis*/drug therapy
beta-Lactamases*/genetics
Anti-Bacterial Agents/pharmacology ; Anti-Bacterial Agents/therapeutic use ; Bacterial Proteins/genetics ; Cephamycins ; Humans ; Klebsiella pneumoniae/enzymology ; Klebsiella pneumoniae/genetics ; Microbial Sensitivity Tests
Czasopismo naukowe
Tytuł :
Use of qPCR for the analysis of population heterogeneity and dynamics during Lactobacillus delbrueckii spp. bulgaricus batch fculture.
Autorzy :
Chen S; School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, China.
Gong P; School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, China.
Zhang J; School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, China.
Shan Y; School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, China.
Han X; School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, China.
Zhang L; School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, China.; College of Food Science and Engineering, Ocean University of China, Qingdao, China.
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Źródło :
Artificial cells, nanomedicine, and biotechnology [Artif Cells Nanomed Biotechnol] 2021 Dec; Vol. 49 (1), pp. 1-10.
Typ publikacji :
Journal Article; Video-Audio Media
MeSH Terms :
Lactobacillus delbrueckii*/genetics
Lactobacillus delbrueckii*/growth & development
RNA, Bacterial*/genetics
RNA, Bacterial*/metabolism
RNA, Ribosomal, 16S*/genetics
RNA, Ribosomal, 16S*/metabolism
Real-Time Polymerase Chain Reaction*
Czasopismo naukowe
Tytuł :
Effect of different storage conditions on COVID-19 RT-PCR results.
Autorzy :
Yilmaz Gulec E; Department of Medical Genetics, Istanbul Kanuni Sultan Suleyman Training and Research Hospital, Health Sciences University, Istanbul, Turkey.; Department of COVID-19 Diagnostic Center, Istanbul Kanuni Sultan Suleyman Training and Research Hospital, Health Sciences University, Istanbul, Turkey.
Cesur NP; Department of COVID-19 Diagnostic Center, TUSEB, Turkey Institutes of Health COVID-19 Diagnostic Center, Istanbul Kanuni Sultan Suleyman Training and Research Hospital, Health Sciences University, Istanbul, Turkey.; Department of Molecular Biology and Genetics, Faculty of Arts and Sciences, Yıldız Technical University, Istanbul, Turkey.
Yesilyurt Fazlioğlu G; Department of COVID-19 Diagnostic Center, TUSEB, Turkey Institutes of Health COVID-19 Diagnostic Center, Istanbul Kanuni Sultan Suleyman Training and Research Hospital, Health Sciences University, Istanbul, Turkey.; Division of Zoology, Department of Biology, Faculty of Sciences, Istanbul University, Istanbul, Turkey.
Kazezoğlu C; Department of COVID-19 Diagnostic Center, Istanbul Kanuni Sultan Suleyman Training and Research Hospital, Health Sciences University, Istanbul, Turkey.; Department of Biochemistry, Central Diagnostic Laboratory, Istanbul Kanuni Sultan Suleyman Training and Research Hospital, Health Sciences University, Istanbul, Turkey.
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Źródło :
Journal of medical virology [J Med Virol] 2021 Dec; Vol. 93 (12), pp. 6575-6581. Date of Electronic Publication: 2021 Jul 28.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
COVID-19 Testing/*methods
Real-Time Polymerase Chain Reaction/*methods
Specimen Handling/*methods
COVID-19/diagnosis ; Humans ; Real-Time Polymerase Chain Reaction/standards ; SARS-CoV-2 ; Specimen Handling/standards ; Time Factors
Czasopismo naukowe
Tytuł :
A DNA intercalating dye-based RT-qPCR alternative to diagnose SARS-CoV-2.
Autorzy :
Fuchs Wightman F; Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, Argentina.; Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE-UBA-CONICET), Ciudad Universitaria, Buenos Aires, Argentina.
Godoy Herz MA; Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, Argentina.; Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE-UBA-CONICET), Ciudad Universitaria, Buenos Aires, Argentina.
Muñoz JC; Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, Argentina.; Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE-UBA-CONICET), Ciudad Universitaria, Buenos Aires, Argentina.
Stigliano JN; Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, Argentina.; Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE-UBA-CONICET), Ciudad Universitaria, Buenos Aires, Argentina.
Bragado L; Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, Argentina.; Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE-UBA-CONICET), Ciudad Universitaria, Buenos Aires, Argentina.
Moreno NN; Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, Argentina.; Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE-UBA-CONICET), Ciudad Universitaria, Buenos Aires, Argentina.
Palavecino M; Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE-UBA-CONICET), Ciudad Universitaria, Buenos Aires, Argentina.; Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, Argentina.
Servi L; Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, Argentina.; Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE-UBA-CONICET), Ciudad Universitaria, Buenos Aires, Argentina.
Cabrerizo G; Instituto de Investigaciones Biomédicas en Retrovirus y SIDA (INBIRS), Universidad de Buenos Aires, Buenos Aires, Argentina.
Clemente J; Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE-UBA-CONICET), Ciudad Universitaria, Buenos Aires, Argentina.; Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, Argentina.
Avaro M; National Influenza Centre PAHO/WHO, Servicio Virosis Respiratorias, Departamento Virología, Instituto Nacional de Enfermedades Infecciosas, Buenos Aires, Argentina.
Pontoriero A; National Influenza Centre PAHO/WHO, Servicio Virosis Respiratorias, Departamento Virología, Instituto Nacional de Enfermedades Infecciosas, Buenos Aires, Argentina.
Benedetti E; National Influenza Centre PAHO/WHO, Servicio Virosis Respiratorias, Departamento Virología, Instituto Nacional de Enfermedades Infecciosas, Buenos Aires, Argentina.
Baumeister E; National Influenza Centre PAHO/WHO, Servicio Virosis Respiratorias, Departamento Virología, Instituto Nacional de Enfermedades Infecciosas, Buenos Aires, Argentina.
Rudolf F; Department of Biosystems Science and Engineering, ETH Zurich, Basel, Switzerland.; SIB Swiss Institute of Bioinformatics, ETH Zurich, Basel, Switzerland.
Remes Lenicov F; Instituto de Investigaciones Biomédicas en Retrovirus y SIDA (INBIRS), Universidad de Buenos Aires, Buenos Aires, Argentina.
Garcia C; Facultad de Ciencias Exactas y Naturales, Departamento de Química Biológica, Universidad De Buenos Aires, Buenos Aires, Argentina.; Laboratorio de Estrategias Antivirales- IQUIBICEN, CONICET-Universidad de Buenos Aires, Buenos Aires, Argentina.
Buggiano V; Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE-UBA-CONICET), Ciudad Universitaria, Buenos Aires, Argentina.; Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, Argentina.
Kornblihtt AR; Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, Argentina.; Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE-UBA-CONICET), Ciudad Universitaria, Buenos Aires, Argentina.
Srebrow A; Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, Argentina.; Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE-UBA-CONICET), Ciudad Universitaria, Buenos Aires, Argentina.
de la Mata M; Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, Argentina.; Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE-UBA-CONICET), Ciudad Universitaria, Buenos Aires, Argentina.
Muñoz MJ; Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, Argentina.; Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE-UBA-CONICET), Ciudad Universitaria, Buenos Aires, Argentina.; Fondazione Istituto FIRC di Oncologia Molecolare (IFOM), Milan, Italy.; Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, Argentina.
Schor IE; Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, Argentina.; Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE-UBA-CONICET), Ciudad Universitaria, Buenos Aires, Argentina.
Petrillo E; Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE-UBA-CONICET), Ciudad Universitaria, Buenos Aires, Argentina.; Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, Argentina.; Instituto Multidisciplinario de Investigación en Patologías Pediátricas (IMIPP), CONICET - GCBA, Buenos Aires, Argentina.
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Źródło :
RNA biology [RNA Biol] 2021 Dec; Vol. 18 (12), pp. 2218-2225. Date of Electronic Publication: 2021 May 26.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Benzothiazoles/*chemistry
COVID-19/*diagnosis
COVID-19 Nucleic Acid Testing/*methods
Diamines/*chemistry
Intercalating Agents/*chemistry
Quinolines/*chemistry
RNA, Viral/*genetics
Real-Time Polymerase Chain Reaction/*methods
SARS-CoV-2/*genetics
COVID-19/virology ; COVID-19 Nucleic Acid Testing/standards ; DNA/analysis ; DNA/biosynthesis ; DNA Primers/chemistry ; DNA Primers/metabolism ; Humans ; Nasopharynx/virology ; Real-Time Polymerase Chain Reaction/standards ; Sensitivity and Specificity
Czasopismo naukowe
Tytuł :
Validation of a novel molecular assay to the diagnostic of COVID-19 based on real time PCR with high resolution melting.
Autorzy :
Ferreira BIDS; Real Time PCR Platform RPT09A, Laboratory of Molecular Biology and Endemic Diseases, Oswaldo Cruz Institute/ Fiocruz, Rio de Janeiro, Brazil.
da Silva-Gomes NL; Real Time PCR Platform RPT09A, Laboratory of Molecular Biology and Endemic Diseases, Oswaldo Cruz Institute/ Fiocruz, Rio de Janeiro, Brazil.
Coelho WLDCNP; Laboratory of Technological Development in Virology, Oswaldo Cruz Institute/ Fiocruz, Rio de Janeiro, Brazil.
da Costa VD; Laboratory of Viral Hepatitis, Oswaldo Cruz Institute/ Fiocruz, Rio de Janeiro, Brazil.
Carneiro VCS; Laboratory of Molecular Virology, Oswaldo Cruz Institute/ Fiocruz, Rio de Janeiro, Brazil.
Kader RL; University Hospital Clementino Fraga Filho, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
Amaro MP; University Hospital Clementino Fraga Filho, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
Villar LM; Laboratory of Viral Hepatitis, Oswaldo Cruz Institute/ Fiocruz, Rio de Janeiro, Brazil.
Miyajima F; Oswaldo Cruz Foundation (Fiocruz), Branch Ceará, Eusebio, Brazil.
Alves-Leon SV; University Hospital Clementino Fraga Filho, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
de Paula VS; Laboratory of Molecular Virology, Oswaldo Cruz Institute/ Fiocruz, Rio de Janeiro, Brazil.
Leon LAA; Laboratory of Technological Development in Virology, Oswaldo Cruz Institute/ Fiocruz, Rio de Janeiro, Brazil.
Moreira OC; Real Time PCR Platform RPT09A, Laboratory of Molecular Biology and Endemic Diseases, Oswaldo Cruz Institute/ Fiocruz, Rio de Janeiro, Brazil.
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Źródło :
PloS one [PLoS One] 2021 Nov 22; Vol. 16 (11), pp. e0260087. Date of Electronic Publication: 2021 Nov 22 (Print Publication: 2021).
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't; Validation Study
MeSH Terms :
COVID-19 Nucleic Acid Testing/*methods
Real-Time Polymerase Chain Reaction/*methods
Adult ; COVID-19 Nucleic Acid Testing/standards ; Female ; Humans ; Male ; Nucleic Acid Denaturation ; Oligonucleotides/chemistry ; Real-Time Polymerase Chain Reaction/standards ; Respiratory Mucosa/virology ; SARS-CoV-2/genetics ; SARS-CoV-2/pathogenicity ; Saliva/virology ; Sensitivity and Specificity
Czasopismo naukowe
Tytuł :
Ultrasensitive Detection of Bacillus anthracis by Real-Time PCR Targeting a Polymorphism in Multi-Copy 16S rRNA Genes and Their Transcripts.
Autorzy :
Braun P; Bundeswehr Institute of Microbiology (IMB), 80937 Munich, Germany.
Nguyen MD; Bundeswehr Institute of Microbiology (IMB), 80937 Munich, Germany.
Walter MC; Bundeswehr Institute of Microbiology (IMB), 80937 Munich, Germany.
Grass G; Bundeswehr Institute of Microbiology (IMB), 80937 Munich, Germany.
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Źródło :
International journal of molecular sciences [Int J Mol Sci] 2021 Nov 12; Vol. 22 (22). Date of Electronic Publication: 2021 Nov 12.
Typ publikacji :
Journal Article
MeSH Terms :
Polymorphism, Genetic*
Real-Time Polymerase Chain Reaction*
Bacillus anthracis/*genetics
DNA, Bacterial/*genetics
DNA, Ribosomal/*genetics
RNA, Bacterial/*genetics
RNA, Ribosomal, 16S/*genetics
Czasopismo naukowe
Tytuł :
A Quadruplex qPCR for Detection and Differentiation of Classic and Natural Recombinant Myxoma Virus Strains of Leporids.
Autorzy :
Abade Dos Santos FA; Centro de Investigação Interdisciplinar em Sanidade Animal (CIISA), Faculdade de Medicina Veterinária, Universidade de Lisboa, Avenida da Universidade Técnica, 1300-477 Lisboa, Portugal.; Instituto Nacional de Investigação Agrária e Veterinária (INIAV, I.P.), Av. da República, Quinta do Marquês, 2780-157 Oeiras, Portugal.; Instituto Universitario de Biotecnología de Asturias (IUBA), Departamento de Bioquímica y Biología Molecular, Universidad de Oviedo, 33003 Oviedo, Spain.
Carvalho CL; Instituto Nacional de Investigação Agrária e Veterinária (INIAV, I.P.), Av. da República, Quinta do Marquês, 2780-157 Oeiras, Portugal.
Parra F; Instituto Universitario de Biotecnología de Asturias (IUBA), Departamento de Bioquímica y Biología Molecular, Universidad de Oviedo, 33003 Oviedo, Spain.
Dalton KP; Instituto Universitario de Biotecnología de Asturias (IUBA), Departamento de Bioquímica y Biología Molecular, Universidad de Oviedo, 33003 Oviedo, Spain.
Peleteiro MC; Centro de Investigação Interdisciplinar em Sanidade Animal (CIISA), Faculdade de Medicina Veterinária, Universidade de Lisboa, Avenida da Universidade Técnica, 1300-477 Lisboa, Portugal.
Duarte MD; Centro de Investigação Interdisciplinar em Sanidade Animal (CIISA), Faculdade de Medicina Veterinária, Universidade de Lisboa, Avenida da Universidade Técnica, 1300-477 Lisboa, Portugal.; Instituto Nacional de Investigação Agrária e Veterinária (INIAV, I.P.), Av. da República, Quinta do Marquês, 2780-157 Oeiras, Portugal.
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Źródło :
International journal of molecular sciences [Int J Mol Sci] 2021 Nov 07; Vol. 22 (21). Date of Electronic Publication: 2021 Nov 07.
Typ publikacji :
Evaluation Study; Journal Article
MeSH Terms :
Myxoma virus*/classification
Myxoma virus*/genetics
Lagomorpha/*virology
Myxomatosis, Infectious/*diagnosis
Real-Time Polymerase Chain Reaction/*methods
Animals ; Animals, Wild ; Diagnosis, Differential ; Gene Transfer, Horizontal/genetics ; Molecular Typing/methods ; Molecular Typing/veterinary ; Myxomatosis, Infectious/virology ; Rabbits ; Real-Time Polymerase Chain Reaction/veterinary ; Reproducibility of Results ; Sensitivity and Specificity ; Spain
Czasopismo naukowe
Tytuł :
Comparison of the current abattoir surveillance system for detection of paratuberculosis in Australian sheep with quantitative PCR tissue strategies using simulation modelling.
Autorzy :
Ly A; Faculty of Science, Sydney School of Veterinary Science, The University of Sydney, Camden, NSW, Australia.
Kirkeby C; Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Frederiksberg, Denmark.
Sergeant ESG; Ausvet Pty Ltd., Canberra, Australia.
Plain KM; Faculty of Science, Sydney School of Veterinary Science, The University of Sydney, Camden, NSW, Australia.
Smith M; Faculty of Science, Sydney School of Veterinary Science, The University of Sydney, Camden, NSW, Australia.
Dhand NK; Faculty of Science, Sydney School of Veterinary Science, The University of Sydney, Camden, NSW, Australia. Electronic address: .
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Źródło :
Preventive veterinary medicine [Prev Vet Med] 2021 Nov; Vol. 196, pp. 105495. Date of Electronic Publication: 2021 Sep 15.
Typ publikacji :
Comparative Study; Journal Article
MeSH Terms :
Paratuberculosis*/diagnosis
Paratuberculosis*/epidemiology
Real-Time Polymerase Chain Reaction*/veterinary
Sheep Diseases*/diagnosis
Sheep Diseases*/epidemiology
Abattoirs ; Animals ; Australia/epidemiology ; Sheep
Czasopismo naukowe
Tytuł :
A rapid and low-cost protocol for the detection of B.1.1.7 lineage of SARS-CoV-2 by using SYBR Green-based RT-qPCR.
Autorzy :
Abdel Sater F; Laboratory of Molecular Biology and Cancer Immunology (Covid 19 Unit), Faculty of Science I, Lebanese University, Hadath, Beirut, 1003, Lebanon.
Younes M; Research Department, Beirut Cardiac Institute, Old Airport Road, Beirut, Lebanon.
Nassar H; Bahman Hospital, Beirut, Lebanon.
Nguewa P; Department of Microbiology and Parasitology, ISTUN Instituto de Salud Tropical, IdiSNA (Navarra Institute for Health Research), University of Navarra, c/ Irunlarrea 1, 31008, Pamplona, Navarra, Spain. .
Hamze K; Laboratory of Molecular Biology and Cancer Immunology (Covid 19 Unit), Faculty of Science I, Lebanese University, Hadath, Beirut, 1003, Lebanon. .
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Źródło :
Molecular biology reports [Mol Biol Rep] 2021 Nov; Vol. 48 (11), pp. 7243-7249. Date of Electronic Publication: 2021 Oct 06.
Typ publikacji :
Journal Article; Validation Study
MeSH Terms :
Benzothiazoles*
Diamines*
Fluorescent Dyes*
Quinolines*
COVID-19/*virology
Real-Time Polymerase Chain Reaction/*methods
SARS-CoV-2/*isolation & purification
COVID-19/diagnosis ; Costs and Cost Analysis ; DNA Primers ; Genome, Viral ; Humans ; Mutation ; Real-Time Polymerase Chain Reaction/economics ; SARS-CoV-2/genetics ; Sensitivity and Specificity ; Spike Glycoprotein, Coronavirus/genetics ; Time Factors
Czasopismo naukowe
Tytuł :
SARS-CoV-2 detection in nasopharyngeal swabs: Performance characteristics of a real-time RT-qPCR and a droplet digital RT-PCR assay based on the exonuclease region (ORF1b, nsp 14).
Autorzy :
Pierri B; Centro di Referenza Nazionale per l'analisi e studio di correlazione tra ambiente, animale e uomo, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Portici, NA, Italy; Department of Medicine, Surgery and Dentistry 'Scuola Medica Salernitana', University of Salerno, Baronissi, SA, Italy.
Mancusi A; Department of Food Microbiology, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Portici, NA, Italy.
Proroga YTR; Department of Food Microbiology, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Portici, NA, Italy.
Capuano F; Department of Food Microbiology, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Portici, NA, Italy.
Cerino P; Centro di Referenza Nazionale per l'analisi e studio di correlazione tra ambiente, animale e uomo, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Portici, NA, Italy.
Girardi S; Department of Food Microbiology, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Portici, NA, Italy.
Vassallo L; Centro di Referenza Nazionale per l'analisi e studio di correlazione tra ambiente, animale e uomo, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Portici, NA, Italy.
Lo Conte G; Centro di Referenza Nazionale per l'analisi e studio di correlazione tra ambiente, animale e uomo, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Portici, NA, Italy.
Tafuro M; Centro di Referenza Nazionale per l'analisi e studio di correlazione tra ambiente, animale e uomo, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Portici, NA, Italy.
Cuomo MC; Centro di Referenza Nazionale per l'analisi e studio di correlazione tra ambiente, animale e uomo, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Portici, NA, Italy.
Di Concilio D; Centro di Referenza Nazionale per l'analisi e studio di correlazione tra ambiente, animale e uomo, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Portici, NA, Italy.
Vicenza T; Department of Food Safety, Nutrition and Veterinary Public Health, Istituto Superiore di Sanità, Rome, Italy.
Cozzi L; Department of Food Safety, Nutrition and Veterinary Public Health, Istituto Superiore di Sanità, Rome, Italy.
Di Pasquale S; Department of Food Safety, Nutrition and Veterinary Public Health, Istituto Superiore di Sanità, Rome, Italy.
La Rosa G; Department of Environment and Health, Istituto Superiore di Sanità, Rome, Italy.
Beikpour F; Department of Food Safety, Nutrition and Veterinary Public Health, Istituto Superiore di Sanità, Rome, Italy; Department of Veterinary Medicine, University of Bari 'Aldo Moro', Bari, Italy.
Suffredini E; Department of Food Safety, Nutrition and Veterinary Public Health, Istituto Superiore di Sanità, Rome, Italy. Electronic address: .
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Źródło :
Journal of virological methods [J Virol Methods] 2022 Feb; Vol. 300, pp. 114420. Date of Electronic Publication: 2021 Dec 10.
Typ publikacji :
Evaluation Study; Journal Article
MeSH Terms :
COVID-19*/diagnosis
Real-Time Polymerase Chain Reaction*
Reverse Transcriptase Polymerase Chain Reaction*
SARS-CoV-2*/isolation & purification
Exonucleases ; Humans ; Nasopharynx/virology ; RNA, Viral/genetics ; Sensitivity and Specificity
Czasopismo naukowe
Tytuł :
Validation of three qPCR for the detection of Burkholderia mallei in equine tissue samples.
Autorzy :
Fonseca Júnior AA; Laboratório Federal de Defesa Agropecuária Em Minas Gerais, Pedro Leopoldo, Brazil. .
Pinto CA; Laboratório Federal de Defesa Agropecuária Em Minas Gerais, Pedro Leopoldo, Brazil.
Alencar CAS; Laboratório Federal de Defesa Agropecuária Em Pernambuco, Recife, Brazil.
Bueno BL; Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
Dos Reis JKP; Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
de Carvalho Filho MB; Laboratório Federal de Defesa Agropecuária Em Minas Gerais, Pedro Leopoldo, Brazil.
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Źródło :
Archives of microbiology [Arch Microbiol] 2021 Sep; Vol. 203 (7), pp. 3965-3971. Date of Electronic Publication: 2021 May 24.
Typ publikacji :
Journal Article; Validation Study
MeSH Terms :
Burkholderia mallei*/genetics
Glanders*/diagnosis
Real-Time Polymerase Chain Reaction*/standards
Real-Time Polymerase Chain Reaction*/veterinary
Animals ; Horses ; Reproducibility of Results
Czasopismo naukowe
Tytuł :
Rethinking COVID-19 test sensitivity-a strategy for improving the detection limit.
Autorzy :
Akhtar MK; Department of Chemistry, College of Science, United Arab Emirates University, Al Ain, United Arab Emirates.
Leung RK; Stanley Ho Centre for Emerging Infectious Diseases, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong, China.
Khan G; Medical Microbiology and Immunology, College of Medicine and Health Sciences, United Arab Emirates University, Al Ain, United Arab Emirates.; Zayed Center for Health Sciences, United Arab Emirates University, Al Ain, United Arab Emirates.
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Źródło :
The Pan African medical journal [Pan Afr Med J] 2021 Aug 16; Vol. 39, pp. 244. Date of Electronic Publication: 2021 Aug 16 (Print Publication: 2021).
Typ publikacji :
Journal Article
MeSH Terms :
COVID-19/*diagnosis
COVID-19 Testing/*methods
Real-Time Polymerase Chain Reaction/*methods
COVID-19 Testing/standards ; False Negative Reactions ; Humans ; Limit of Detection ; Real-Time Polymerase Chain Reaction/standards ; Sensitivity and Specificity ; Specimen Handling
Czasopismo naukowe
Tytuł :
Case Study Using Recommended Reference Genes Actin and 18S for Reverse-Transcription Quantitative Real-Time PCR Analysis in Myzus persicae.
Autorzy :
Rahman S; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, PR China.
Zhao Z; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, PR China.
Umair Sial M; Institute of Plant Protection, Muhammad Nawaz Shareef University of Agriculture, Multan, Pakistan.
Zhang Y; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, PR China.
Jiang H; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, PR China.
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Źródło :
PloS one [PLoS One] 2021 Oct 20; Vol. 16 (10), pp. e0258201. Date of Electronic Publication: 2021 Oct 20 (Print Publication: 2021).
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Genes, Insect*
Real-Time Polymerase Chain Reaction*
Reverse Transcriptase Polymerase Chain Reaction*
Actins/*genetics
Aphids/*genetics
RNA, Ribosomal, 18S/*genetics
Animals ; Aphids/growth & development ; Gene Expression Regulation, Developmental/drug effects ; Insecticides/toxicity ; Niacinamide/analogs & derivatives ; Niacinamide/toxicity ; Reference Standards ; Triazines/toxicity
Czasopismo naukowe
Tytuł :
Real-time PCR method for qualitative and quantitative detection of Lactobacillus sakei group species targeting novel markers based on bioinformatics analysis.
Autorzy :
Kim E; Institute of Life Sciences & Resources and Department of Food Science and Biotechnology, Kyung Hee University, Yongin 17104, Republic of Korea.
Yang SM; Institute of Life Sciences & Resources and Department of Food Science and Biotechnology, Kyung Hee University, Yongin 17104, Republic of Korea.
Kim D; Institute of Life Sciences & Resources and Department of Food Science and Biotechnology, Kyung Hee University, Yongin 17104, Republic of Korea.
Kim HY; Institute of Life Sciences & Resources and Department of Food Science and Biotechnology, Kyung Hee University, Yongin 17104, Republic of Korea. Electronic address: .
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Źródło :
International journal of food microbiology [Int J Food Microbiol] 2021 Oct 02; Vol. 355, pp. 109335. Date of Electronic Publication: 2021 Jul 26.
Typ publikacji :
Journal Article
MeSH Terms :
Computational Biology*
Lactobacillus sakei*/genetics
Real-Time Polymerase Chain Reaction*
Fermented Foods/microbiology ; Genetic Markers/genetics ; RNA, Ribosomal, 16S/genetics
Czasopismo naukowe
Tytuł :
Detection of Mycobacterium tuberculosis complex in pulmonary and extrapulmonary samples with the FluoroType MTBDR assay.
Autorzy :
Svensson E; International Reference Laboratory of Mycobacteriology, Statens Serum Institut, Copenhagen, Denmark. Electronic address: .
Folkvardsen DB; International Reference Laboratory of Mycobacteriology, Statens Serum Institut, Copenhagen, Denmark.
Rasmussen EM; International Reference Laboratory of Mycobacteriology, Statens Serum Institut, Copenhagen, Denmark.
Lillebaek T; International Reference Laboratory of Mycobacteriology, Statens Serum Institut, Copenhagen, Denmark; Global Health Section, Department of Public Health, University of Copenhagen, Copenhagen, Denmark.
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Źródło :
Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases [Clin Microbiol Infect] 2021 Oct; Vol. 27 (10), pp. 1514.e1-1514.e4. Date of Electronic Publication: 2021 Jan 06.
Typ publikacji :
Journal Article
MeSH Terms :
Mycobacterium tuberculosis*/genetics
Mycobacterium tuberculosis*/isolation & purification
Real-Time Polymerase Chain Reaction*
Tuberculosis*/diagnosis
Antitubercular Agents/pharmacology ; Bacterial Proteins/genetics ; Humans ; Isoniazid/pharmacology ; Mutation ; Rifampin/pharmacology ; Sensitivity and Specificity
Czasopismo naukowe

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