Temat: "Real-time polymerase chain reaction" - OpacWWW

Skocz do pozycji: 1.

Tytuł:: High-resolution melt curve analysis: An approach for variant detection in the TPO gene of congenital hypothyroid patients in Bangladesh.
Autorzy:: Begum MN; Department of Genetic Engineering & Biotechnology, University of Dhaka, Dhaka, Bangladesh.; Institute for Developing Science and Health Initiatives (ideSHi), ECB Chattar, Mirpur, Dhaka, Bangladesh.; Infectious Diseases Division, Virology Laboratory, International Centre for Diarrhoeal Disease Research, Bangladesh, Mohakhali, Dhaka, Bangladesh.
Mahtarin R; Institute for Developing Science and Health Initiatives (ideSHi), ECB Chattar, Mirpur, Dhaka, Bangladesh.
Islam MT; Institute for Developing Science and Health Initiatives (ideSHi), ECB Chattar, Mirpur, Dhaka, Bangladesh.
Antora NJ; Institute for Developing Science and Health Initiatives (ideSHi), ECB Chattar, Mirpur, Dhaka, Bangladesh.
Sarker SK; Institute for Developing Science and Health Initiatives (ideSHi), ECB Chattar, Mirpur, Dhaka, Bangladesh.
Sultana N; Institute for Developing Science and Health Initiatives (ideSHi), ECB Chattar, Mirpur, Dhaka, Bangladesh.
Sajib AA; Department of Genetic Engineering & Biotechnology, University of Dhaka, Dhaka, Bangladesh.
Islam ABMMK; Department of Genetic Engineering & Biotechnology, University of Dhaka, Dhaka, Bangladesh.
Banu H; Department of Endocrinology, Bangabandhu Sheikh Mujib Medical University (BSMMU), Shahbag, Dhaka, Bangladesh.
Hasanat MA; Department of Endocrinology, Bangabandhu Sheikh Mujib Medical University (BSMMU), Shahbag, Dhaka, Bangladesh.
Shyamaly KJ; Department of Pediatrics, Bangabandhu Sheikh Mujib Medical University (BSMMU) Shahbag, Dhaka, Bangladesh.
Begum S; Department of Pediatrics, Bangabandhu Sheikh Mujib Medical University (BSMMU) Shahbag, Dhaka, Bangladesh.
Konika TK; Nuclear Medicine and Allied Sciences, Bangabandhu Sheikh Mujib Medical University (BSMMU), Shahbag, Dhaka, Bangladesh.
Haque S; Nuclear Medicine and Allied Sciences, Bangabandhu Sheikh Mujib Medical University (BSMMU), Shahbag, Dhaka, Bangladesh.
Hasan M; Nuclear Medicine and Allied Sciences, Bangabandhu Sheikh Mujib Medical University (BSMMU), Shahbag, Dhaka, Bangladesh.
Sultana S; Scintigraphy Division, Bangabandhu Sheikh Mujib Medical University (BSMMU) Shahbag, Dhaka, Bangladesh.
Bhuiyan TR; Infectious Diseases Division, Mucosal Immunology and Vaccinology, International Centre for Diarrhoeal Disease Research, Bangladesh, Mohakhali, Dhaka, Bangladesh.
Mannoor K; Institute for Developing Science and Health Initiatives (ideSHi), ECB Chattar, Mirpur, Dhaka, Bangladesh.
Qadri F; Institute for Developing Science and Health Initiatives (ideSHi), ECB Chattar, Mirpur, Dhaka, Bangladesh.; Infectious Diseases Division, Mucosal Immunology and Vaccinology, International Centre for Diarrhoeal Disease Research, Bangladesh, Mohakhali, Dhaka, Bangladesh.
Akhteruzzaman S; Department of Genetic Engineering & Biotechnology, University of Dhaka, Dhaka, Bangladesh.; Pokaż więcej
Źródło:: PloS one [PLoS One] 2024 Apr 10; Vol. 19 (4), pp. e0293570. Date of Electronic Publication: 2024 Apr 10 (Print Publication: 2024).
Typ publikacji:: Journal Article
MeSH Terms:: Congenital Hypothyroidism*/diagnosis
Congenital Hypothyroidism*/genetics
Humans ; Bangladesh ; Mutation ; DNA ; Real-Time Polymerase Chain Reaction

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Skocz do pozycji: 2.

Tytuł:: EndoGeneAnalyzer: A tool for selection and validation of reference genes.
Autorzy:: Teixeira EB; Oncology Research Center, Federal University of Pará, Belém, Pará, Brazil.
Khayat AS; Oncology Research Center, Federal University of Pará, Belém, Pará, Brazil.
Assumpção PP; Oncology Research Center, Federal University of Pará, Belém, Pará, Brazil.
Casseb SM; Oncology Research Center, Federal University of Pará, Belém, Pará, Brazil.
Moreira-Nunes CA; Oncology Research Center, Federal University of Pará, Belém, Pará, Brazil.; Pharmacogenetics Laboratory, Drug Research and Development Center (NPDM), Department of Medicine, Federal University of Ceará, Fortaleza, Brazil.; Clementino Fraga Group, Central Unity, Molecular Biology Laboratory, Fortaleza, CE, Brazil.
Moreira FC; Oncology Research Center, Federal University of Pará, Belém, Pará, Brazil.; Pokaż więcej
Źródło:: PloS one [PLoS One] 2024 Apr 03; Vol. 19 (4), pp. e0299993. Date of Electronic Publication: 2024 Apr 03 (Print Publication: 2024).
Typ publikacji:: Journal Article
MeSH Terms:: Gene Expression Profiling*
Real-Time Polymerase Chain Reaction ; Reference Standards ; Reverse Transcriptase Polymerase Chain Reaction

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Skocz do pozycji: 3.

Tytuł:: A quantitative assay for the assessment of cutaneous human papillomaviruses and polyomaviruses over time: A proof-of-concept in two patients with atopic dermatitis and psoriasis.
Autorzy:: Du-Thanh A; Pathogen Discovery Laboratory, Institut Pasteur, Paris, France.; Pathogenesis and Control of Chronic Infections, INSERM, University of Montpellier, Montpellier, France.; Département de Dermatologie, CHU de Montpellier, Montpellier, France.
Foulongne V; Pathogenesis and Control of Chronic Infections, INSERM, University of Montpellier, Montpellier, France.; Laboratoire de Virologie, CHU de Montpellier, Montpellier, France.
Dereure O; Pathogenesis and Control of Chronic Infections, INSERM, University of Montpellier, Montpellier, France.; Département de Dermatologie, CHU de Montpellier, Montpellier, France.
Eloit M; Pathogen Discovery Laboratory, Institut Pasteur, Paris, France.; Ecole Nationale Vétérinaire d'Alfort, Maisons-Alfort, France.
Pérot P; Pathogen Discovery Laboratory, Institut Pasteur, Paris, France.; Pokaż więcej
Źródło:: PloS one [PLoS One] 2024 Apr 03; Vol. 19 (4), pp. e0297907. Date of Electronic Publication: 2024 Apr 03 (Print Publication: 2024).
Typ publikacji:: Case Reports; Journal Article
MeSH Terms:: Polyomavirus*/genetics
Dermatitis, Atopic*
Papillomavirus Infections*
Psoriasis*
Skin Diseases*
Merkel cell polyomavirus*
Humans ; Human Papillomavirus Viruses ; DNA, Viral/genetics ; DNA, Viral/analysis ; Skin/microbiology ; Papillomaviridae/genetics ; Real-Time Polymerase Chain Reaction

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Skocz do pozycji: 4.

Tytuł:: Detection of pathogenic Leptospira with rapid extraction followed by recombinase polymerase amplification (RPA) and quantitative polymerase chain reaction (qPCR) assay-A comprehensive study from Sri Lanka.
Autorzy:: Uduwawala H; Molecular Medicine Unit, Faculty of Medicine, University of Kelaniya, Ragama, Sri Lanka.
Manamperi A; Molecular Medicine Unit, Faculty of Medicine, University of Kelaniya, Ragama, Sri Lanka.
Gunaratna GPS; Department of Medical Microbiology, Faculty of Medicine, University of Kelaniya, Ragama, Sri Lanka.
Karunanayake L; National Reference Laboratory for Leptospirosis, Medical Research Institute, Colombo, Sri Lanka.
Ceruti A; Institute of Animal Hygiene and Veterinary Public Health, Leipzig University, Leipzig, Germany.
Abd El Wahed A; Institute of Animal Hygiene and Veterinary Public Health, Leipzig University, Leipzig, Germany.
Fernando L; Centre for Clinical Management of Dengue & Dengue Haemorrhagic Fever, District General Hospital, Negombo, Sri Lanka.
Premaratna R; Department of Medicine, Faculty of Medicine, University of Kelaniya, Ragama, Sri Lanka.
Hapugoda M; Molecular Medicine Unit, Faculty of Medicine, University of Kelaniya, Ragama, Sri Lanka.; Pokaż więcej
Źródło:: PloS one [PLoS One] 2024 Mar 15; Vol. 19 (3), pp. e0295287. Date of Electronic Publication: 2024 Mar 15 (Print Publication: 2024).
Typ publikacji:: Journal Article
MeSH Terms:: Leptospira*/genetics
Leptospirosis*/diagnosis
Animals ; Humans ; Recombinases ; Retrospective Studies ; Prospective Studies ; Sri Lanka ; Polymerase Chain Reaction ; Nucleotidyltransferases ; Zoonoses ; Sensitivity and Specificity ; Real-Time Polymerase Chain Reaction/methods ; Nucleic Acid Amplification Techniques/methods

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Skocz do pozycji: 5.

Tytuł:: Investigating animal reservoirs for hepatitis E virus in Bangui, Central African Republic.
Autorzy:: Nombot-Yazenguet MPM; Viral Hepatitis Laboratory, Institut Pasteur de Bangui, Bangui, Central African Republic.
Modiyinji AF; Department of Virology, Centre Pasteur of Cameroon, Yaoundé, Cameroon.
Tricou V; Viral Hepatitis Laboratory, Institut Pasteur de Bangui, Bangui, Central African Republic.
Manirakiza A; Epidemiological Service, Institut Pasteur de Bangui, Bangui, Central African Republic.
Njouom R; Department of Virology, Centre Pasteur of Cameroon, Yaoundé, Cameroon.
Komas NPJ; Viral Hepatitis Laboratory, Institut Pasteur de Bangui, Bangui, Central African Republic.; Pokaż więcej
Źródło:: PloS one [PLoS One] 2024 Mar 15; Vol. 19 (3), pp. e0300608. Date of Electronic Publication: 2024 Mar 15 (Print Publication: 2024).
Typ publikacji:: Journal Article
MeSH Terms:: Hepatitis E virus*/genetics
Hepatitis E*/epidemiology
Hepatitis E*/veterinary
Swine Diseases*
Swine ; Animals ; Humans ; Phylogeny ; Central African Republic/epidemiology ; Prospective Studies ; Real-Time Polymerase Chain Reaction ; RNA, Viral/genetics ; RNA, Viral/analysis ; Genotype ; Feces/chemistry ; Goats/genetics

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Skocz do pozycji: 6.

Tytuł:: Effect of target gene sequence evenness and dominance on real-time PCR quantification of artificial sulfate-reducing microbial communities.
Autorzy:: Du Z; Center for Environmental Health Risk Assessment and Research, Chinese Research Academy of Environmental Sciences, Beijing, China.; The BioTechnology Institute, University of Minnesota Twin Cities, St. Paul, Minnesota, United States of America.
Behrens SF; The BioTechnology Institute, University of Minnesota Twin Cities, St. Paul, Minnesota, United States of America.; Department of Civil, Environmental, and Geo-Engineering, University of Minnesota Twin Cities, Minneapolis, Minnesota, United States of America.; Pokaż więcej
Źródło:: PloS one [PLoS One] 2024 Mar 07; Vol. 19 (3), pp. e0299930. Date of Electronic Publication: 2024 Mar 07 (Print Publication: 2024).
Typ publikacji:: Journal Article
MeSH Terms:: Sulfates*
Microbiota*/genetics
Real-Time Polymerase Chain Reaction/methods ; Phylogeny ; Reproducibility of Results

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Skocz do pozycji: 7.

Tytuł:: Clinical epidemiology, determinants, and outcomes of viral encephalitis in Ghana; a cross-sectional study.
Autorzy:: Yeboah R; Kumasi Centre for Collaborative Research in Tropical Medicine, Kumasi, Ghana.; Department of Theoretical and Applied Biology, Kwame Nkrumah University of Science and Technology (KNUST), Kumasi, Ghana.
Gorman R; Kumasi Centre for Collaborative Research in Tropical Medicine, Kumasi, Ghana.
Acheampong HK; Kumasi Centre for Collaborative Research in Tropical Medicine, Kumasi, Ghana.
Nyarko-Afriyie E; Kumasi Centre for Collaborative Research in Tropical Medicine, Kumasi, Ghana.
Aryeetey S; Kumasi Centre for Collaborative Research in Tropical Medicine, Kumasi, Ghana.
Tetteh HD; Kumasi Centre for Collaborative Research in Tropical Medicine, Kumasi, Ghana.
Owusu M; Kumasi Centre for Collaborative Research in Tropical Medicine, Kumasi, Ghana.; Department of Medical Diagnostics, Kwame Nkrumah University of Science and Technology (KNUST), Kumasi, Ghana.
Yeboah ES; Emergency Unit, Nyaho Medical Centre, Accra, Ghana.
Adade T; Department of Medicine, Komfo Anokye Teaching Hospital, Kumasi, Ghana.
Bonney J; Kumasi Centre for Collaborative Research in Tropical Medicine, Kumasi, Ghana.; Emergency Medicine Directorate, Komfo Anokye Teaching Hospital, Kumasi, Ghana.
Amoako YA; Kumasi Centre for Collaborative Research in Tropical Medicine, Kumasi, Ghana.; Department of Medicine, Komfo Anokye Teaching Hospital, Kumasi, Ghana.; Department of Medicine, School of Medicine and Dentistry, Kwame Nkrumah University of Science and Technology (KNUST), Kumasi, Ghana.
El-Duah P; Institute of Virology, Charite, Universitätsmedizin Berlin, Berlin, Germany.
Obiri-Danso K; Department of Theoretical and Applied Biology, Kwame Nkrumah University of Science and Technology (KNUST), Kumasi, Ghana.
Drosten C; Institute of Virology, Charite, Universitätsmedizin Berlin, Berlin, Germany.
Phillips RO; Kumasi Centre for Collaborative Research in Tropical Medicine, Kumasi, Ghana.; Department of Medicine, Komfo Anokye Teaching Hospital, Kumasi, Ghana.; Department of Medicine, School of Medicine and Dentistry, Kwame Nkrumah University of Science and Technology (KNUST), Kumasi, Ghana.
Sylverken AA; Kumasi Centre for Collaborative Research in Tropical Medicine, Kumasi, Ghana.; Department of Theoretical and Applied Biology, Kwame Nkrumah University of Science and Technology (KNUST), Kumasi, Ghana.; Pokaż więcej
Źródło:: PloS one [PLoS One] 2024 Feb 12; Vol. 19 (2), pp. e0297277. Date of Electronic Publication: 2024 Feb 12 (Print Publication: 2024).
Typ publikacji:: Journal Article
MeSH Terms:: Encephalitis, Viral*/diagnosis
Encephalitis, Viral*/epidemiology
Viruses*
Humans ; Cross-Sectional Studies ; Ghana/epidemiology ; Prospective Studies ; Real-Time Polymerase Chain Reaction

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Skocz do pozycji: 8.

Tytuł:: COVID-19 in patients presenting with malaria-like symptoms at a primary healthcare facility in Accra, Ghana.
Autorzy:: Asamoah I; Department of Medical Microbiology, University of Ghana Medical School, University of Ghana, Korle-Bu, Accra, Ghana.
Adusei-Poku M; Department of Medical Microbiology, University of Ghana Medical School, University of Ghana, Korle-Bu, Accra, Ghana.
Vandyck-Sey P; Korle Bu Polyclinic Family Medicine Department, Korle Bu Teaching Hospital, Accra, Ghana.
Steele-Dadzie A; Korle Bu Polyclinic Family Medicine Department, Korle Bu Teaching Hospital, Accra, Ghana.
Kuffour AS; Department of Medical Microbiology, University of Ghana Medical School, University of Ghana, Korle-Bu, Accra, Ghana.
Turkson A; Department of Medical Microbiology, University of Ghana Medical School, University of Ghana, Korle-Bu, Accra, Ghana.
Asante IA; Department of Virology, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra, Ghana.
Addo-Osafo K; Department of Medical Microbiology, University of Ghana Medical School, University of Ghana, Korle-Bu, Accra, Ghana.
Mohktar Q; Department of Immunology, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra, Ghana.
Adu B; Department of Immunology, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra, Ghana.
Afrane YA; Department of Medical Microbiology, University of Ghana Medical School, University of Ghana, Korle-Bu, Accra, Ghana.
Sagoe KWC; Department of Medical Microbiology, University of Ghana Medical School, University of Ghana, Korle-Bu, Accra, Ghana.; Pokaż więcej
Źródło:: PloS one [PLoS One] 2024 Feb 09; Vol. 19 (2), pp. e0298088. Date of Electronic Publication: 2024 Feb 09 (Print Publication: 2024).
Typ publikacji:: Journal Article
MeSH Terms:: COVID-19*/diagnosis
COVID-19*/epidemiology
Malaria*/diagnosis
Malaria*/epidemiology
Humans ; SARS-CoV-2/genetics ; COVID-19 Testing ; Ghana/epidemiology ; Real-Time Polymerase Chain Reaction ; Headache ; Primary Health Care ; Sensitivity and Specificity
SCR Organism:: SARS-CoV-2 variants

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Skocz do pozycji: 9.

Tytuł:: Selection and validation of reference genes for RT-qPCR in ophiocordyceps sinensis under different experimental conditions.
Autorzy:: He L; School of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong University, Lanzhou, GanSu, P. R. China.
Wang JY; School of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong University, Lanzhou, GanSu, P. R. China.
Su QJ; School of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong University, Lanzhou, GanSu, P. R. China.
Chen ZH; School of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong University, Lanzhou, GanSu, P. R. China.
Xie F; School of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong University, Lanzhou, GanSu, P. R. China.; Pokaż więcej
Źródło:: PloS one [PLoS One] 2024 Feb 06; Vol. 19 (2), pp. e0287882. Date of Electronic Publication: 2024 Feb 06 (Print Publication: 2024).
Typ publikacji:: Journal Article
MeSH Terms:: Cordyceps*/genetics
RNA, Ribosomal, 18S/genetics ; Gene Expression Profiling/methods ; Genes, Plant ; Real-Time Polymerase Chain Reaction/methods ; Reference Standards ; Tubulin/genetics ; Ubiquitin/genetics

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Skocz do pozycji: 10.

Tytuł:: Re-testing as a method of implementing external quality assessment program for COVID-19 real time PCR testing in Uganda.
Autorzy:: Okek EJ; Department of Arbovirology, Uganda Virus Research Institute, Entebbe, Uganda.; College of Health Sciences, Makerere University, Kampala, Uganda.; Viral Hemorrhagic Fevers Laboratory, Uganda Virus Research Institute, Entebbe, Uganda.
Masembe FJ; College of Health Sciences, Makerere University, Kampala, Uganda.
Kiconco J; Department of Arbovirology, Uganda Virus Research Institute, Entebbe, Uganda.
Kayiwa J; Department of Arbovirology, Uganda Virus Research Institute, Entebbe, Uganda.
Amwine E; Department of Arbovirology, Uganda Virus Research Institute, Entebbe, Uganda.; Viral Hemorrhagic Fevers Laboratory, Uganda Virus Research Institute, Entebbe, Uganda.
Obote D; College of Health Sciences, Makerere University, Kampala, Uganda.
Alele S; College of Health Sciences, Makerere University, Kampala, Uganda.
Nahabwe C; Department of Quality Assurance, Allied Health Professional Council, Kampala, Uganda.
Were J; Department of Diagnostics, Mulago National Referral Hospital, Kampala, Uganda.
Bagaya B; College of Health Sciences, Makerere University, Kampala, Uganda.
Balinandi S; Department of Arbovirology, Uganda Virus Research Institute, Entebbe, Uganda.; Viral Hemorrhagic Fevers Laboratory, Uganda Virus Research Institute, Entebbe, Uganda.
Lutwama J; Department of Arbovirology, Uganda Virus Research Institute, Entebbe, Uganda.; College of Health Sciences, Makerere University, Kampala, Uganda.; Viral Hemorrhagic Fevers Laboratory, Uganda Virus Research Institute, Entebbe, Uganda.
Kaleebu P; Department of Arbovirology, Uganda Virus Research Institute, Entebbe, Uganda.; College of Health Sciences, Makerere University, Kampala, Uganda.; Viral Hemorrhagic Fevers Laboratory, Uganda Virus Research Institute, Entebbe, Uganda.; Pokaż więcej
Źródło:: PloS one [PLoS One] 2024 Jan 24; Vol. 19 (1), pp. e0287272. Date of Electronic Publication: 2024 Jan 24 (Print Publication: 2024).
Typ publikacji:: Journal Article
MeSH Terms:: COVID-19*/diagnosis
Humans ; Uganda ; Real-Time Polymerase Chain Reaction ; COVID-19 Testing ; Academies and Institutes

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Skocz do pozycji: 11.

Tytuł:: Single-tube multiplex real-time PCR with EvaGreen and high-resolution melting analysis for diagnosis of α0-thalassemia--SEA,--THAI, and--CR type deletions.
Autorzy:: Ruengdit C; Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand.
Punyamung M; Associated Medical Sciences Clinical Service Center, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand.
Intasai N; Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand.
Pornprasert S; Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand.; Pokaż więcej
Źródło:: PloS one [PLoS One] 2023 Nov 06; Vol. 18 (11), pp. e0293838. Date of Electronic Publication: 2023 Nov 06 (Print Publication: 2023).
Typ publikacji:: Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:: alpha-Thalassemia*/genetics
Hemoglobins, Abnormal*/genetics
Pregnancy ; Female ; Humans ; Thailand ; Real-Time Polymerase Chain Reaction ; Southeast Asian People ; Hydrops Fetalis/diagnosis ; Hydrops Fetalis/genetics ; Prenatal Diagnosis/methods

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Skocz do pozycji: 12.

Tytuł:: Screening of C. auris among Candida isolates from various tertiary care institutions in Lahore by VITEK 2 and real time PCR based molecular technique.
Autorzy:: Huma ZE; Department of Microbiology, University of Health Sciences, Lahore, Pakistan.
Saleem S; Department of Microbiology, University of Health Sciences, Lahore, Pakistan.
Imran M; Department of Microbiology, University of Health Sciences, Lahore, Pakistan.
Jabeen K; Department of Microbiology, University of Health Sciences, Lahore, Pakistan.
Arshad F; Department of Microbiology, University of Health Sciences, Lahore, Pakistan.
Amar A; Department of Human Genetics and Molecular Biology, University of Health Sciences, Lahore, Pakistan.; Pokaż więcej
Źródło:: PloS one [PLoS One] 2023 Oct 24; Vol. 18 (10), pp. e0293390. Date of Electronic Publication: 2023 Oct 24 (Print Publication: 2023).
Typ publikacji:: Journal Article
MeSH Terms:: Candidiasis*/diagnosis
Candidiasis*/epidemiology
Cross Infection*/diagnosis
Cross Infection*/epidemiology
Humans ; Candida ; Real-Time Polymerase Chain Reaction ; Fluconazole/pharmacology ; Tertiary Healthcare ; Hospitals, Teaching ; Microbial Sensitivity Tests ; Antifungal Agents

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Skocz do pozycji: 13.

Tytuł:: Comparison of DNA concentration and bacterial pathogen PCR detection when using two DNA extraction kits for nasopharyngeal/oropharyngeal samples.
Autorzy:: Khan D; Medical Research Council Unit The Gambia at the London School of Hygiene & Tropical Medicine, Banjul, The Gambia.
Thomas SA; Medical Research Council Unit The Gambia at the London School of Hygiene & Tropical Medicine, Banjul, The Gambia.
Tientcheu PE; Medical Research Council Unit The Gambia at the London School of Hygiene & Tropical Medicine, Banjul, The Gambia.
Suso SMS; Medical Research Council Unit The Gambia at the London School of Hygiene & Tropical Medicine, Banjul, The Gambia.
Dupont C; J. Craig Venter Institute, La Jolla, CA, United States of America.
Kwambana-Adams B; Medical Research Council Unit The Gambia at the London School of Hygiene & Tropical Medicine, Banjul, The Gambia.; NIHR Global Health Research Unit on Mucosal Pathogens, Division of Infection and Immunity, University College London, London, United Kingdom.
Mohammed NI; Medical Research Council Unit The Gambia at the London School of Hygiene & Tropical Medicine, Banjul, The Gambia.
Nicol MP; Division of Infection and Immunity, School of Biomedical Sciences, University of Western Australia, Perth, Australia.
Antonio M; Medical Research Council Unit The Gambia at the London School of Hygiene & Tropical Medicine, Banjul, The Gambia.; Centre for Epidemic Preparedness and Response, London School of Hygiene & Tropical Medicine, London, United Kingdom.; Department of Infection Biology, Faculty of Infectious and Tropical Diseases, London School of Hygiene & Tropical Medicine, London, United Kingdom.; Pokaż więcej
Źródło:: PloS one [PLoS One] 2023 Aug 03; Vol. 18 (8), pp. e0289557. Date of Electronic Publication: 2023 Aug 03 (Print Publication: 2023).
Typ publikacji:: Journal Article; Research Support, N.I.H., Extramural
MeSH Terms:: Bacteria*/genetics
Neisseria meningitidis*/genetics
Humans ; DNA ; Streptococcus pneumoniae/genetics ; Real-Time Polymerase Chain Reaction ; Nasopharynx ; Oropharynx ; Haemophilus influenzae/genetics ; DNA, Bacterial/genetics

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Skocz do pozycji: 14.

Tytuł:: Development and Evaluation of AccuPower COVID-19 Multiplex Real-Time RT-PCR Kit and AccuPower SARS-CoV-2 Multiplex Real-Time RT-PCR Kit for SARS-CoV-2 Detection in Sputum, NPS/OPS, Saliva and Pooled Samples.
Autorzy:: Suh IB; Department of Laboratory Medicine, College of Medicine, Kangwon National University, Chuncheon, Korea.
Lim J; Department of Laboratory Medicine, Myongji Hospital, Hanyang University College of Medicine, Goyang, Korea.
Kim HS; Research Administration Team, Institute of Clinical Medicine, Myongji Hospital, Hanyang University College of Medicine, Goyang, Korea.
Rhim G; Department of General Affairs, Korean Association of Otorhinolaryngologists, Seoul, Korea.
Kim H; Department of Laboratory Medicine, College of Medicine, Kangwon National University, Chuncheon, Korea.
Kim H; Department of Laboratory Medicine, College of Medicine, Kangwon National University, Chuncheon, Korea.
Lee SM; Department of Laboratory Medicine, College of Medicine, Kangwon National University, Chuncheon, Korea.
Park HS; Department of Laboratory Medicine, College of Medicine, Kangwon National University, Chuncheon, Korea.
Song HJ; Department of Laboratory Medicine, College of Medicine, Kangwon National University, Chuncheon, Korea.
Hong M; Department of Laboratory Medicine, College of Medicine, Kangwon National University, Chuncheon, Korea.
Shin GS; Department of Laboratory Medicine, College of Medicine, Kangwon National University, Chuncheon, Korea.
Kim MJ; Department of Laboratory Medicine, Myongji Hospital, Hanyang University College of Medicine, Goyang, Korea.; Pokaż więcej
Źródło:: PloS one [PLoS One] 2022 Feb 10; Vol. 17 (2), pp. e0263341. Date of Electronic Publication: 2022 Feb 10 (Print Publication: 2022).
Typ publikacji:: Evaluation Study; Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:: Multiplex Polymerase Chain Reaction*
Real-Time Polymerase Chain Reaction*
COVID-19/*virology
Nasopharynx/*virology
Oropharynx/*virology
SARS-CoV-2/*genetics
SARS-CoV-2/*isolation & purification
Saliva/*virology
Sputum/*virology
Cross Reactions ; Humans ; Limit of Detection ; Sensitivity and Specificity

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Skocz do pozycji: 15.

Tytuł:: A robust biostatistical method leverages informative but uncertainly determined qPCR data for biomarker detection, early diagnosis, and treatment.
Autorzy:: Zhuang W; Division of Bioinformatics and Biostatistics, National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, Arkansas, United States of America.
Camacho L; Division of Biochemical Toxicology, National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, Arkansas, United States of America.
Silva CS; Division of Biochemical Toxicology, National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, Arkansas, United States of America.
Thomson M; Office of New Drugs, Center for Drug Evaluation and Research, U.S. Food and Drug Administration, Silver Spring, Maryland, United States of America.
Snyder K; Office of New Drugs, Center for Drug Evaluation and Research, U.S. Food and Drug Administration, Silver Spring, Maryland, United States of America.; Pokaż więcej
Źródło:: PloS one [PLoS One] 2022 Jan 31; Vol. 17 (1), pp. e0263070. Date of Electronic Publication: 2022 Jan 31 (Print Publication: 2022).
Typ publikacji:: Journal Article; Research Support, U.S. Gov't, P.H.S.
MeSH Terms:: Early Diagnosis*
Models, Theoretical*
Real-Time Polymerase Chain Reaction*
Biomarkers ; Humans

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Skocz do pozycji: 16.

Tytuł:: Development and comparison of loop-mediated isothermal amplification with quantitative PCR for the specific detection of Saprolegnia spp.
Autorzy:: Ghosh S; Department of Biological Sciences, Bowling Green State University, Bowling Green, Ohio, United States of America.
Straus DL; United States Department of Agriculture, Agricultural Research Service, Harry K. Dupree-Stuttgart National Aquaculture Research Center, Stuttgart, Arkansas, United States of America.
Good C; The Conservation Fund's Freshwater Institute, Shepherdstown, West Virginia, United States of America.
Phuntumart V; Department of Biological Sciences, Bowling Green State University, Bowling Green, Ohio, United States of America.; Pokaż więcej
Źródło:: PloS one [PLoS One] 2021 Dec 13; Vol. 16 (12), pp. e0250808. Date of Electronic Publication: 2021 Dec 13 (Print Publication: 2021).
Typ publikacji:: Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:: Molecular Diagnostic Techniques*
Nucleic Acid Amplification Techniques*
Real-Time Polymerase Chain Reaction*
DNA Primers/*genetics
Electron Transport Complex IV/*genetics
Saprolegnia/*genetics
Saprolegnia/classification
SCR Protocol:: LAMP assay

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Skocz do pozycji: 17.

Tytuł:: Validation of a novel molecular assay to the diagnostic of COVID-19 based on real time PCR with high resolution melting.
Autorzy:: Ferreira BIDS; Real Time PCR Platform RPT09A, Laboratory of Molecular Biology and Endemic Diseases, Oswaldo Cruz Institute/ Fiocruz, Rio de Janeiro, Brazil.
da Silva-Gomes NL; Real Time PCR Platform RPT09A, Laboratory of Molecular Biology and Endemic Diseases, Oswaldo Cruz Institute/ Fiocruz, Rio de Janeiro, Brazil.
Coelho WLDCNP; Laboratory of Technological Development in Virology, Oswaldo Cruz Institute/ Fiocruz, Rio de Janeiro, Brazil.
da Costa VD; Laboratory of Viral Hepatitis, Oswaldo Cruz Institute/ Fiocruz, Rio de Janeiro, Brazil.
Carneiro VCS; Laboratory of Molecular Virology, Oswaldo Cruz Institute/ Fiocruz, Rio de Janeiro, Brazil.
Kader RL; University Hospital Clementino Fraga Filho, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
Amaro MP; University Hospital Clementino Fraga Filho, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
Villar LM; Laboratory of Viral Hepatitis, Oswaldo Cruz Institute/ Fiocruz, Rio de Janeiro, Brazil.
Miyajima F; Oswaldo Cruz Foundation (Fiocruz), Branch Ceará, Eusebio, Brazil.
Alves-Leon SV; University Hospital Clementino Fraga Filho, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
de Paula VS; Laboratory of Molecular Virology, Oswaldo Cruz Institute/ Fiocruz, Rio de Janeiro, Brazil.
Leon LAA; Laboratory of Technological Development in Virology, Oswaldo Cruz Institute/ Fiocruz, Rio de Janeiro, Brazil.
Moreira OC; Real Time PCR Platform RPT09A, Laboratory of Molecular Biology and Endemic Diseases, Oswaldo Cruz Institute/ Fiocruz, Rio de Janeiro, Brazil.; Pokaż więcej
Źródło:: PloS one [PLoS One] 2021 Nov 22; Vol. 16 (11), pp. e0260087. Date of Electronic Publication: 2021 Nov 22 (Print Publication: 2021).
Typ publikacji:: Journal Article; Research Support, Non-U.S. Gov't; Validation Study
MeSH Terms:: COVID-19 Nucleic Acid Testing/*methods
Real-Time Polymerase Chain Reaction/*methods
Adult ; COVID-19 Nucleic Acid Testing/standards ; Female ; Humans ; Male ; Nucleic Acid Denaturation ; Oligonucleotides/chemistry ; Real-Time Polymerase Chain Reaction/standards ; Respiratory Mucosa/virology ; SARS-CoV-2/genetics ; SARS-CoV-2/pathogenicity ; Saliva/virology ; Sensitivity and Specificity

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Skocz do pozycji: 18.

Tytuł:: Case Study Using Recommended Reference Genes Actin and 18S for Reverse-Transcription Quantitative Real-Time PCR Analysis in Myzus persicae.
Autorzy:: Rahman S; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, PR China.
Zhao Z; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, PR China.
Umair Sial M; Institute of Plant Protection, Muhammad Nawaz Shareef University of Agriculture, Multan, Pakistan.
Zhang Y; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, PR China.
Jiang H; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, PR China.; Pokaż więcej
Źródło:: PloS one [PLoS One] 2021 Oct 20; Vol. 16 (10), pp. e0258201. Date of Electronic Publication: 2021 Oct 20 (Print Publication: 2021).
Typ publikacji:: Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:: Genes, Insect*
Real-Time Polymerase Chain Reaction*
Reverse Transcriptase Polymerase Chain Reaction*
Actins/*genetics
Aphids/*genetics
RNA, Ribosomal, 18S/*genetics
Animals ; Aphids/growth & development ; Gene Expression Regulation, Developmental/drug effects ; Insecticides/toxicity ; Niacinamide/analogs & derivatives ; Niacinamide/toxicity ; Reference Standards ; Triazines/toxicity

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Skocz do pozycji: 19.

Tytuł:: Validation of a redesigned pan-poliovirus assay and real-time PCR platforms for the global poliovirus laboratory network.
Autorzy:: Sun H; Division of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America.
Harrington C; Division of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America.
Gerloff N; Division of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America.
Mandelbaum M; Division of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America.
Jeffries-Miles S; Division of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America.
Apostol LNG; Research Institute for Tropical Medicine, Muntinlupa City, Philippines.
Valencia MAD; Research Institute for Tropical Medicine, Muntinlupa City, Philippines.
Shaukat S; National Institute of Health, Islamabad, Pakistan.
Angez M; National Institute of Health, Islamabad, Pakistan.
Sharma DK; National Institute of Virology, Mumbai, India.
Nalavade UP; National Institute of Virology, Mumbai, India.
Pawar SD; National Institute of Virology, Mumbai, India.
Pukuta Simbu E; National Institute of Biomedical Research, Kinshasa, Democratic Republic of the Congo, Congo.
Andriamamonjy S; Virology Unit, Institut Pasteur de Madagascar, Antananarivo, Madagascar.
Razafindratsimandresy R; Virology Unit, Institut Pasteur de Madagascar, Antananarivo, Madagascar.
Vega E; Division of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America.; Pokaż więcej
Źródło:: PloS one [PLoS One] 2021 Aug 06; Vol. 16 (8), pp. e0255795. Date of Electronic Publication: 2021 Aug 06 (Print Publication: 2021).
Typ publikacji:: Journal Article
MeSH Terms:: Poliovirus*
Real-Time Polymerase Chain Reaction*
Feces ; Laboratories ; Sewage

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Skocz do pozycji: 20.

Tytuł:: The selection and validation of reference genes for quantitative real-time PCR studies in near-isogenic susceptible and resistant tomato lines, infected with the geminivirus tomato curly stunt virus.
Autorzy:: Bokhale M; Department of Biochemistry, University of Johannesburg, Auckland Park, Johannesburg, South Africa.
Mwaba I; Department of Biochemistry, University of Johannesburg, Auckland Park, Johannesburg, South Africa.
Allie F; Department of Biochemistry, University of Johannesburg, Auckland Park, Johannesburg, South Africa.; Pokaż więcej
Źródło:: PloS one [PLoS One] 2023 Jul 27; Vol. 18 (7), pp. e0284456. Date of Electronic Publication: 2023 Jul 27 (Print Publication: 2023).
Typ publikacji:: Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:: Solanum lycopersicum*/genetics
Geminiviridae*/genetics
Real-Time Polymerase Chain Reaction/methods ; Genes, Plant ; Gene Expression Profiling/methods ; Transferases/genetics ; Reference Standards

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