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Wyszukujesz frazę ""Receptors, Mating Factor"" wg kryterium: Temat


Tytuł :
Dielectric Spectroscopy Based Detection of Specific and Nonspecific Cellular Mechanisms.
Autorzy :
Stoneman MR; Department of Physics, University of Wisconsin-Milwaukee, Milwaukee, WI 53211, USA.
Raicu V; Department of Physics, University of Wisconsin-Milwaukee, Milwaukee, WI 53211, USA.; Department of Biological Sciences, University of Wisconsin-Milwaukee, Milwaukee, WI 53211, USA.
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Źródło :
Sensors (Basel, Switzerland) [Sensors (Basel)] 2021 May 03; Vol. 21 (9). Date of Electronic Publication: 2021 May 03.
Typ publikacji :
Journal Article
MeSH Terms :
Dielectric Spectroscopy*
Mating Factor*
Saccharomyces cerevisiae Proteins*
Cell Membrane ; Receptors, Mating Factor ; Saccharomyces cerevisiae
Czasopismo naukowe
Tytuł :
Structure of the class D GPCR Ste2 dimer coupled to two G proteins.
Autorzy :
Velazhahan V; MRC Laboratory of Molecular Biology, Cambridge, UK.
Ma N; Department of Computational and Quantitative Medicine, Beckman Research Institute of the City of Hope, Duarte, CA, USA.
Pándy-Szekeres G; Department of Drug Design and Pharmacology, Universitetsparken 2, Copenhagen, Denmark.; Medicinal Chemistry Research Group, Research Center for Natural Sciences, Budapest, Hungary.
Kooistra AJ; Department of Drug Design and Pharmacology, Universitetsparken 2, Copenhagen, Denmark.
Lee Y; MRC Laboratory of Molecular Biology, Cambridge, UK.
Gloriam DE; Department of Drug Design and Pharmacology, Universitetsparken 2, Copenhagen, Denmark.
Vaidehi N; Department of Computational and Quantitative Medicine, Beckman Research Institute of the City of Hope, Duarte, CA, USA.
Tate CG; MRC Laboratory of Molecular Biology, Cambridge, UK. .
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Źródło :
Nature [Nature] 2021 Jan; Vol. 589 (7840), pp. 148-153. Date of Electronic Publication: 2020 Dec 02.
Typ publikacji :
Comparative Study; Journal Article
MeSH Terms :
Cryoelectron Microscopy*
Protein Multimerization*
Heterotrimeric GTP-Binding Proteins/*chemistry
Heterotrimeric GTP-Binding Proteins/*metabolism
Receptors, Mating Factor/*chemistry
Receptors, Mating Factor/*metabolism
Saccharomyces cerevisiae/*chemistry
Saccharomyces cerevisiae/*metabolism
Saccharomyces cerevisiae Proteins/*chemistry
Saccharomyces cerevisiae Proteins/*metabolism
Amino Acid Sequence ; Binding Sites ; GTP-Binding Protein alpha Subunits, Gq-G11/chemistry ; GTP-Binding Protein alpha Subunits, Gq-G11/metabolism ; GTP-Binding Protein beta Subunits/chemistry ; GTP-Binding Protein beta Subunits/metabolism ; GTP-Binding Protein gamma Subunits/chemistry ; GTP-Binding Protein gamma Subunits/metabolism ; Humans ; Models, Molecular ; Protein Precursors/metabolism ; Sequence Alignment
Czasopismo naukowe
Tytuł :
In-Cell Detection of Conformational Substates of a G Protein-Coupled Receptor Quaternary Structure: Modulation of Substate Probability by Cognate Ligand Binding.
Autorzy :
Paprocki J; Department of Physics, University of Wisconsin-Milwaukee, Milwaukee, Wisconsin 53211, United States.
Biener G; Department of Physics, University of Wisconsin-Milwaukee, Milwaukee, Wisconsin 53211, United States.
Stoneman M; Department of Physics, University of Wisconsin-Milwaukee, Milwaukee, Wisconsin 53211, United States.
Raicu V; Department of Physics, University of Wisconsin-Milwaukee, Milwaukee, Wisconsin 53211, United States.; Department of Biological Sciences, University of Wisconsin-Milwaukee, Milwaukee, Wisconsin 53211, United States.
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Źródło :
The journal of physical chemistry. B [J Phys Chem B] 2020 Nov 12; Vol. 124 (45), pp. 10062-10076. Date of Electronic Publication: 2020 Oct 29.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
MeSH Terms :
Fluorescence Resonance Energy Transfer*
Receptors, G-Protein-Coupled*/metabolism
GTP-Binding Proteins ; Ligands ; Probability ; Receptors, Mating Factor/genetics ; Receptors, Mating Factor/metabolism
Czasopismo naukowe
Tytuł :
Ste2 receptor-mediated chemotropism of Fusarium graminearum contributes to its pathogenicity against wheat.
Autorzy :
Sridhar PS; Department of Biomedical and Molecular Sciences, Queen's University, 18 Stuart St., Kingston, ON, K7L 3N6, Canada.
Trofimova D; Department of Biomedical and Molecular Sciences, Queen's University, 18 Stuart St., Kingston, ON, K7L 3N6, Canada.
Subramaniam R; Agriculture and Agri-Food Canada, 960 Carling Avenue, Ottawa, ON, K1A 0C6, Canada.
González-Peña Fundora D; Agriculture and Agri-Food Canada, 5403, 1st Avenue South, Lethbridge, AB, T1J 4B1, Canada.
Foroud NA; Agriculture and Agri-Food Canada, 5403, 1st Avenue South, Lethbridge, AB, T1J 4B1, Canada.
Allingham JS; Department of Biomedical and Molecular Sciences, Queen's University, 18 Stuart St., Kingston, ON, K7L 3N6, Canada.
Loewen MC; Department of Biomedical and Molecular Sciences, Queen's University, 18 Stuart St., Kingston, ON, K7L 3N6, Canada. .; National Research Council of Canada, 100 Sussex Drive, Ottawa, ON, K1A 0R6, Canada. .
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Źródło :
Scientific reports [Sci Rep] 2020 Jul 01; Vol. 10 (1), pp. 10770. Date of Electronic Publication: 2020 Jul 01.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Fungal Proteins/*metabolism
Fusarium/*metabolism
Receptors, Mating Factor/*metabolism
Triticum/*microbiology
Agrobacterium tumefaciens ; Catalysis ; Cell Wall/metabolism ; Chemotaxis ; Gene Deletion ; Hyphae/metabolism ; Ligands ; MAP Kinase Signaling System ; Peroxidases/metabolism ; Pheromones/metabolism ; Plant Diseases/microbiology ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae Proteins ; Signal Transduction ; Spores, Fungal/metabolism ; Virulence
SCR Organism :
Fusarium graminearum
Czasopismo naukowe
Tytuł :
Detection of a Peptide Biomarker by Engineered Yeast Receptors.
Autorzy :
Adeniran A; Department of Chemical and Biological Engineering, ‡Interdisciplinary Biological Sciences Graduate Program, Northwestern University , Evanston, Illinois.
Stainbrook S; Department of Chemical and Biological Engineering, ‡Interdisciplinary Biological Sciences Graduate Program, Northwestern University , Evanston, Illinois.
Bostick JW; Department of Chemical and Biological Engineering, ‡Interdisciplinary Biological Sciences Graduate Program, Northwestern University , Evanston, Illinois.
Tyo KEJ; Department of Chemical and Biological Engineering, ‡Interdisciplinary Biological Sciences Graduate Program, Northwestern University , Evanston, Illinois.
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Źródło :
ACS synthetic biology [ACS Synth Biol] 2018 Feb 16; Vol. 7 (2), pp. 696-705. Date of Electronic Publication: 2018 Feb 05.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
MeSH Terms :
Peptides*/chemistry
Peptides*/genetics
Receptors, Mating Factor*/chemistry
Receptors, Mating Factor*/genetics
Saccharomyces cerevisiae*/chemistry
Saccharomyces cerevisiae*/genetics
Saccharomyces cerevisiae Proteins*/chemistry
Saccharomyces cerevisiae Proteins*/genetics
Cystatin C/*analysis
Directed Molecular Evolution/*methods
Protein Engineering/*methods
Biomarkers/chemistry ; Humans
Czasopismo naukowe
Tytuł :
CRISPR-addressable yeast strains with applications in human G protein-coupled receptor profiling and synthetic biology.
Autorzy :
Rowe JB; Department of Molecular and Cellular Pharmacology, University of Miami Miller School of Medicine, Miami, Florida, USA.
Taghon GJ; Department of Molecular and Cellular Pharmacology, University of Miami Miller School of Medicine, Miami, Florida, USA.
Kapolka NJ; Department of Molecular and Cellular Pharmacology, University of Miami Miller School of Medicine, Miami, Florida, USA.
Morgan WM; Department of Molecular and Cellular Pharmacology, University of Miami Miller School of Medicine, Miami, Florida, USA.
Isom DG; Department of Molecular and Cellular Pharmacology, University of Miami Miller School of Medicine, Miami, Florida, USA .; University of Miami Sylvester Comprehensive Cancer Center, Miami, Florida, USA.; University of Miami Center for Computational Sciences, Miami, Florida, USA.
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Źródło :
The Journal of biological chemistry [J Biol Chem] 2020 Jun 12; Vol. 295 (24), pp. 8262-8271. Date of Electronic Publication: 2020 May 01.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural
MeSH Terms :
Synthetic Biology*
Clustered Regularly Interspaced Short Palindromic Repeats/*genetics
Receptors, G-Protein-Coupled/*metabolism
Saccharomyces cerevisiae/*metabolism
Autocrine Communication ; Gene Dosage ; Genes, Reporter ; Humans ; Metabolic Engineering ; Pheromones/metabolism ; Receptors, Mating Factor/metabolism ; Receptors, Somatostatin/metabolism ; Reproducibility of Results ; Saccharomyces cerevisiae Proteins/metabolism ; Signal Transduction ; Somatostatin/analogs & derivatives ; Somatostatin/pharmacology
Czasopismo naukowe
Tytuł :
Quaternary structure of the yeast pheromone receptor Ste2 in living cells.
Autorzy :
Stoneman MR; Physics Department, University of Wisconsin-Milwaukee, Milwaukee, WI, USA. Electronic address: .
Paprocki JD; Physics Department, University of Wisconsin-Milwaukee, Milwaukee, WI, USA.
Biener G; Physics Department, University of Wisconsin-Milwaukee, Milwaukee, WI, USA.
Yokoi K; Physics Department, University of Wisconsin-Milwaukee, Milwaukee, WI, USA.
Shevade A; Department of Biological Sciences, University of Wisconsin-Milwaukee, Milwaukee, WI, USA.
Kuchin S; Department of Biological Sciences, University of Wisconsin-Milwaukee, Milwaukee, WI, USA.
Raicu V; Physics Department, University of Wisconsin-Milwaukee, Milwaukee, WI, USA; Department of Biological Sciences, University of Wisconsin-Milwaukee, Milwaukee, WI, USA. Electronic address: .
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Źródło :
Biochimica et biophysica acta. Biomembranes [Biochim Biophys Acta Biomembr] 2017 Sep; Vol. 1859 (9 Pt A), pp. 1456-1464. Date of Electronic Publication: 2016 Dec 16.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
MeSH Terms :
Receptors, G-Protein-Coupled/*chemistry
Receptors, Mating Factor/*chemistry
Receptors, Pheromone/*chemistry
Saccharomyces cerevisiae Proteins/*chemistry
Cell Membrane/chemistry ; Cell Membrane/metabolism ; Fluorescence Resonance Energy Transfer ; Pheromones/metabolism ; Protein Multimerization ; Protein Structure, Quaternary ; Receptors, G-Protein-Coupled/genetics ; Receptors, G-Protein-Coupled/metabolism ; Receptors, Mating Factor/genetics ; Receptors, Mating Factor/metabolism ; Receptors, Pheromone/genetics ; Receptors, Pheromone/metabolism ; Saccharomyces cerevisiae/genetics ; Saccharomyces cerevisiae Proteins/genetics ; Saccharomyces cerevisiae Proteins/metabolism
Czasopismo naukowe
Tytuł :
GPCR-Gα protein precoupling: Interaction between Ste2p, a yeast GPCR, and Gpa1p, its Gα protein, is formed before ligand binding via the Ste2p C-terminal domain and the Gpa1p N-terminal domain.
Autorzy :
Cevheroğlu O; Department of Microbiology, University of Tennessee, Knoxville, TN 37996-0845, United States; Department of Biological Sciences, Middle East Technical University, Universiteler Mah. Dumlupinar Blv. No: 1, Çankaya, Ankara, 06800, Turkey.
Becker JM; Department of Microbiology, University of Tennessee, Knoxville, TN 37996-0845, United States.
Son ÇD; Department of Biological Sciences, Middle East Technical University, Universiteler Mah. Dumlupinar Blv. No: 1, Çankaya, Ankara, 06800, Turkey. Electronic address: .
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Źródło :
Biochimica et biophysica acta. Biomembranes [Biochim Biophys Acta Biomembr] 2017 Dec; Vol. 1859 (12), pp. 2435-2446. Date of Electronic Publication: 2017 Sep 27.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
MeSH Terms :
Cell Membrane/*metabolism
Endoplasmic Reticulum/*metabolism
GTP-Binding Protein alpha Subunits, Gq-G11/*metabolism
Mating Factor/*metabolism
Receptors, Mating Factor/*metabolism
Saccharomyces cerevisiae/*metabolism
Saccharomyces cerevisiae Proteins/*metabolism
Bioluminescence Resonance Energy Transfer Techniques ; Cell Membrane/chemistry ; Endoplasmic Reticulum/chemistry ; GTP-Binding Protein alpha Subunits, Gq-G11/genetics ; Gene Expression ; Genes, Reporter ; Green Fluorescent Proteins/genetics ; Green Fluorescent Proteins/metabolism ; Ligands ; Mating Factor/genetics ; Phosphorylation ; Protein Binding ; Protein Interaction Domains and Motifs ; Protein Multimerization ; Receptors, Mating Factor/genetics ; Saccharomyces cerevisiae/genetics ; Saccharomyces cerevisiae Proteins/genetics ; Signal Transduction
Czasopismo naukowe
Tytuł :
Ratiometric GPCR signaling enables directional sensing in yeast.
Autorzy :
Henderson NT; Department of Pharmacology and Cancer Biology, Duke University, Durham, North Carolina, United States of America.
Pablo M; Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America.; Program in Molecular and Cellular Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America.
Ghose D; Department of Pharmacology and Cancer Biology, Duke University, Durham, North Carolina, United States of America.
Clark-Cotton MR; Department of Pharmacology and Cancer Biology, Duke University, Durham, North Carolina, United States of America.
Zyla TR; Department of Pharmacology and Cancer Biology, Duke University, Durham, North Carolina, United States of America.
Nolen J; Department of Mathematics, Duke University, Durham, North Carolina, United States of America.
Elston TC; Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America.
Lew DJ; Department of Pharmacology and Cancer Biology, Duke University, Durham, North Carolina, United States of America.
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Źródło :
PLoS biology [PLoS Biol] 2019 Oct 17; Vol. 17 (10), pp. e3000484. Date of Electronic Publication: 2019 Oct 17 (Print Publication: 2019).
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
MeSH Terms :
Gene Expression Regulation, Fungal*
Genes, Mating Type, Fungal*
Pheromones/*metabolism
Saccharomyces cerevisiae/*genetics
Signal Transduction/*genetics
Cell Cycle Proteins/genetics ; Cell Cycle Proteins/metabolism ; Cyclin-Dependent Kinase Inhibitor Proteins/genetics ; Cyclin-Dependent Kinase Inhibitor Proteins/metabolism ; Genes, Reporter ; Green Fluorescent Proteins/genetics ; Green Fluorescent Proteins/metabolism ; Guanine Nucleotide Exchange Factors/genetics ; Guanine Nucleotide Exchange Factors/metabolism ; Receptors, Mating Factor/genetics ; Receptors, Mating Factor/metabolism ; Saccharomyces cerevisiae/metabolism ; Saccharomyces cerevisiae Proteins/genetics ; Saccharomyces cerevisiae Proteins/metabolism ; cdc42 GTP-Binding Protein, Saccharomyces cerevisiae/genetics ; cdc42 GTP-Binding Protein, Saccharomyces cerevisiae/metabolism
Czasopismo naukowe
Tytuł :
Variable Dependence of Signaling Output on Agonist Occupancy of Ste2p, a G Protein-coupled Receptor in Yeast.
Autorzy :
Sridharan R; From the Department of Biochemistry and Biophysics, University of Rochester Medical Center, Rochester, New York 14642.
Connelly SM; From the Department of Biochemistry and Biophysics, University of Rochester Medical Center, Rochester, New York 14642.
Naider F; the Department of Chemistry and Macromolecular Assembly Institute, College of Staten Island of the City University of New York, Staten Island, New York 10314, and.; the Ph.D. Programs in Biochemistry and Chemistry, Graduate Center of the City University of New York, New York, New York 10016.
Dumont ME; From the Department of Biochemistry and Biophysics, University of Rochester Medical Center, Rochester, New York 14642, .
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Źródło :
The Journal of biological chemistry [J Biol Chem] 2016 Nov 11; Vol. 291 (46), pp. 24261-24279. Date of Electronic Publication: 2016 Sep 19.
Typ publikacji :
Journal Article
MeSH Terms :
GTPase-Activating Proteins/*metabolism
Receptors, Mating Factor/*metabolism
Saccharomyces cerevisiae/*metabolism
Saccharomyces cerevisiae Proteins/*metabolism
Signal Transduction/*physiology
GTPase-Activating Proteins/genetics ; Receptors, Mating Factor/genetics ; Saccharomyces cerevisiae/genetics ; Saccharomyces cerevisiae Proteins/genetics
Czasopismo naukowe
Tytuł :
Evolution of a G protein-coupled receptor response by mutations in regulatory network interactions.
Autorzy :
Di Roberto RB; Department of Cell and Systems Biology, University of Toronto, 25 Harbord Street, Toronto, Ontario, Canada M5S 3G5.
Chang B; Department of Cell and Systems Biology, University of Toronto, 25 Harbord Street, Toronto, Ontario, Canada M5S 3G5.
Trusina A; Niels Bohr Institute, University of Copenhagen, Blegdamsvej 17, Copenhagen Ø 2100, Denmark.
Peisajovich SG; Department of Cell and Systems Biology, University of Toronto, 25 Harbord Street, Toronto, Ontario, Canada M5S 3G5.
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Źródło :
Nature communications [Nat Commun] 2016 Aug 04; Vol. 7, pp. 12344. Date of Electronic Publication: 2016 Aug 04.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Evolution, Molecular*
Gene Regulatory Networks*/drug effects
Mutation/*genetics
Receptors, Mating Factor/*genetics
Saccharomyces cerevisiae/*genetics
Saccharomyces cerevisiae Proteins/*genetics
GTPase-Activating Proteins/metabolism ; Ligands ; Mitogen-Activated Protein Kinases/metabolism ; Models, Biological ; Pheromones/pharmacology ; Phosphorylation/drug effects ; Protein Binding/drug effects ; Receptors, Mating Factor/metabolism ; Saccharomyces cerevisiae/drug effects ; Saccharomyces cerevisiae/growth & development ; Saccharomyces cerevisiae Proteins/metabolism
Czasopismo naukowe
Tytuł :
The N-terminus of the yeast G protein-coupled receptor Ste2p plays critical roles in surface expression, signaling, and negative regulation.
Autorzy :
Uddin MS; Department of Microbiology, University of Tennessee, Knoxville, TN 37996, United States.
Hauser M; Department of Microbiology, University of Tennessee, Knoxville, TN 37996, United States.
Naider F; Department of Chemistry, and Macromolecular Assemblies Institute, College of Staten Island, CUNY, New York, NY 10314, United States; Graduate School and University Center, CUNY, New York, NY 10314, United States.
Becker JM; Department of Microbiology, University of Tennessee, Knoxville, TN 37996, United States. Electronic address: .
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Źródło :
Biochimica et biophysica acta [Biochim Biophys Acta] 2016 Apr; Vol. 1858 (4), pp. 715-24. Date of Electronic Publication: 2015 Dec 17.
Typ publikacji :
Journal Article
MeSH Terms :
Signal Transduction*
Receptors, G-Protein-Coupled/*chemistry
Receptors, Mating Factor/*chemistry
Saccharomyces cerevisiae Proteins/*chemistry
Amino Acid Sequence ; Binding Sites ; Gene Expression Regulation, Fungal ; Ligands ; Mating Factor ; Peptides/chemistry ; Peptides/metabolism ; Protein Binding ; Protein Structure, Tertiary ; Receptors, G-Protein-Coupled/biosynthesis ; Receptors, G-Protein-Coupled/metabolism ; Receptors, Mating Factor/biosynthesis ; Receptors, Mating Factor/metabolism ; Saccharomyces cerevisiae/chemistry ; Saccharomyces cerevisiae Proteins/biosynthesis ; Saccharomyces cerevisiae Proteins/metabolism ; Surface Properties
Czasopismo naukowe
Tytuł :
Fungal pathogen uses sex pheromone receptor for chemotropic sensing of host plant signals.
Autorzy :
Turrà D; Departamento de Genética, Campus de Excelencia Internacional Agroalimentario ceiA3, Universidad de Córdoba, 14071 Córdoba, Spain.
El Ghalid M; Departamento de Genética, Campus de Excelencia Internacional Agroalimentario ceiA3, Universidad de Córdoba, 14071 Córdoba, Spain.
Rossi F; Departamento de Genética, Campus de Excelencia Internacional Agroalimentario ceiA3, Universidad de Córdoba, 14071 Córdoba, Spain.
Di Pietro A; Departamento de Genética, Campus de Excelencia Internacional Agroalimentario ceiA3, Universidad de Córdoba, 14071 Córdoba, Spain.
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Źródło :
Nature [Nature] 2015 Nov 26; Vol. 527 (7579), pp. 521-4. Date of Electronic Publication: 2015 Oct 26.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Host-Pathogen Interactions*
Fusarium/*metabolism
Lycopersicon esculentum/*metabolism
Lycopersicon esculentum/*microbiology
Peroxidases/*metabolism
Receptors, Mating Factor/*metabolism
Tropism/*physiology
Catalysis ; Fusarium/growth & development ; Hyphae/growth & development ; Hyphae/metabolism ; Lycopersicon esculentum/enzymology ; MAP Kinase Signaling System ; Mating Factor ; Peptides/metabolism ; Plant Roots/enzymology ; Plant Roots/microbiology ; Receptors, Mating Factor/chemistry
Czasopismo naukowe
Tytuł :
A Cellular System for Spatial Signal Decoding in Chemical Gradients.
Autorzy :
Hegemann B; Department of Biology, Institute of Biochemistry, ETH Zurich, Otto-Stern-Weg 3, 8093 Zürich, Switzerland. Electronic address: .
Unger M; Department of Biology, Institute of Biochemistry, ETH Zurich, Otto-Stern-Weg 3, 8093 Zürich, Switzerland; Automatic Control Laboratory, ETH Zurich, Physikstrasse 3, 8092 Zürich, Switzerland.
Lee SS; Department of Biology, Institute of Biochemistry, ETH Zurich, Otto-Stern-Weg 3, 8093 Zürich, Switzerland.
Stoffel-Studer I; Department of Biology, Institute of Biochemistry, ETH Zurich, Otto-Stern-Weg 3, 8093 Zürich, Switzerland.
van den Heuvel J; Department of Biology, Institute of Biochemistry, ETH Zurich, Otto-Stern-Weg 3, 8093 Zürich, Switzerland.
Pelet S; Department of Fundamental Microbiology, University of Lausanne, Biophore Building, 1015 Lausanne, Switzerland.
Koeppl H; Automatic Control Laboratory, ETH Zurich, Physikstrasse 3, 8092 Zürich, Switzerland; Department of Electrical Engineering and Information Technology, Technische Universität Darmstadt, Rundeturmstrasse 12, 64283 Darmstadt, Germany.
Peter M; Department of Biology, Institute of Biochemistry, ETH Zurich, Otto-Stern-Weg 3, 8093 Zürich, Switzerland. Electronic address: .
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Źródło :
Developmental cell [Dev Cell] 2015 Nov 23; Vol. 35 (4), pp. 458-70. Date of Electronic Publication: 2015 Nov 12.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Signal Transduction*
Cell Polarity/*physiology
Mitogen-Activated Protein Kinases/*metabolism
Receptors, Mating Factor/*metabolism
Saccharomyces cerevisiae/*growth & development
Saccharomyces cerevisiae/*metabolism
Saccharomyces cerevisiae Proteins/*metabolism
cdc42 GTP-Binding Protein, Saccharomyces cerevisiae/*metabolism
Chemotaxis ; Image Processing, Computer-Assisted ; Immunoblotting ; Microfluidic Analytical Techniques ; Mitogen-Activated Protein Kinases/genetics ; Models, Theoretical ; Protein Binding ; Protein Transport ; Receptors, Mating Factor/genetics ; Saccharomyces cerevisiae/genetics ; Saccharomyces cerevisiae Proteins/genetics ; Single-Cell Analysis/methods ; cdc42 GTP-Binding Protein, Saccharomyces cerevisiae/genetics
Czasopismo naukowe
Tytuł :
The mapping of yeast's G-protein coupled receptor with an atomic force microscope.
Autorzy :
Takenaka M; Department of Chemical and Engineering, Graduate School of Engineering, Kobe University, Kobe, Japan. .
Miyachi Y
Ishii J
Ogino C
Kondo A
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Źródło :
Nanoscale [Nanoscale] 2015 Mar 21; Vol. 7 (11), pp. 4956-63.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Microscopy, Atomic Force*
Receptors, Mating Factor/*metabolism
Saccharomyces cerevisiae/*metabolism
Saccharomyces cerevisiae Proteins/*metabolism
Carbocyanines/chemistry ; Ligands ; Mating Factor ; Peptides/chemistry ; Peptides/genetics ; Peptides/metabolism ; Receptors, Mating Factor/antagonists & inhibitors ; Receptors, Mating Factor/genetics ; Saccharomyces cerevisiae Proteins/antagonists & inhibitors ; Saccharomyces cerevisiae Proteins/chemistry ; Saccharomyces cerevisiae Proteins/genetics
Czasopismo naukowe
Tytuł :
Identification of destabilizing and stabilizing mutations of Ste2p, a G protein-coupled receptor in Saccharomyces cerevisiae.
Autorzy :
Zuber J; Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry , P.O. Box 712, Rochester, New York 14642, United States.
Danial SA
Connelly SM
Naider F
Dumont ME
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Źródło :
Biochemistry [Biochemistry] 2015 Mar 10; Vol. 54 (9), pp. 1787-806. Date of Electronic Publication: 2015 Feb 24.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
MeSH Terms :
Mutation*
Receptors, Mating Factor/*genetics
Receptors, Mating Factor/*metabolism
Saccharomyces cerevisiae Proteins/*genetics
Saccharomyces cerevisiae Proteins/*metabolism
Amino Acid Substitution ; Mutagenesis, Site-Directed ; Protein Stability ; Protein Structure, Tertiary/genetics ; Receptors, Mating Factor/chemistry ; Saccharomyces cerevisiae Proteins/chemistry ; Signal Transduction/genetics ; Temperature
Czasopismo naukowe
Tytuł :
Development of a Highly Active Fluorescence-Based Detector for Yeast G Protein-Coupled Receptor Ste2p.
Autorzy :
Hong JW; School of Biotechnology, Yeungnam University, Gyeongsan 38541, Republic of Korea.
Ahn HJ; School of Biotechnology, Yeungnam University, Gyeongsan 38541, Republic of Korea.
Baek JS; School of Biotechnology, Yeungnam University, Gyeongsan 38541, Republic of Korea.
Hong EY; School of Biotechnology, Yeungnam University, Gyeongsan 38541, Republic of Korea.
Jin DH; Asan Institute for Life Science, Department of Convergence Medicine, College of Medicine, University of Ulsan 44610, Republic of Korea.
Khang YH; School of Biotechnology, Yeungnam University, Gyeongsan 38541, Republic of Korea.
Hong NJ; School of Biotechnology, Yeungnam University, Gyeongsan 38541, Republic of Korea.
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Źródło :
Journal of microbiology and biotechnology [J Microbiol Biotechnol] 2018 Oct 28; Vol. 28 (10), pp. 1589-1603.
Typ publikacji :
Journal Article
MeSH Terms :
Mating Factor/*analysis
Mating Factor/*metabolism
Receptors, G-Protein-Coupled/*metabolism
Saccharomyces cerevisiae Proteins/*metabolism
Amino Acid Sequence ; Binding Sites ; Binding, Competitive ; Fluorescence ; Gene Expression ; Genes, Reporter/genetics ; Mating Factor/chemical synthesis ; Mating Factor/chemistry ; Protein Binding ; Receptors, G-Protein-Coupled/genetics ; Receptors, Mating Factor/genetics ; Receptors, Mating Factor/metabolism ; Saccharomyces cerevisiae/genetics ; Saccharomyces cerevisiae/growth & development ; Saccharomyces cerevisiae/metabolism ; Saccharomyces cerevisiae Proteins/genetics
Czasopismo naukowe
Tytuł :
Novobiocin and peptide analogs of α-factor are positive allosteric modulators of the yeast G protein-coupled receptor Ste2p.
Autorzy :
Rymer JK; Department of Microbiology, University of Tennessee, Knoxville, TN 37996, United States.
Hauser M; Department of Microbiology, University of Tennessee, Knoxville, TN 37996, United States.
Bourdon AK; Department of Chemistry, University of Tennessee, Knoxville, TN 37996, United States.
Campagna SR; Department of Chemistry, University of Tennessee, Knoxville, TN 37996, United States.
Naider F; Department of Chemistry and Macromolecular Assemblies Institute, College of Staten Island, CUNY, New York, NY 10314, United States; Graduate School and University Center, CUNY, New York, NY 10314, United States.
Becker JM; Department of Microbiology, University of Tennessee, Knoxville, TN 37996, United States. Electronic address: .
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Źródło :
Biochimica et biophysica acta [Biochim Biophys Acta] 2015 Apr; Vol. 1848 (4), pp. 916-24. Date of Electronic Publication: 2015 Jan 07.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural
MeSH Terms :
Allosteric Regulation/*drug effects
Anti-Bacterial Agents/*pharmacology
Novobiocin/*pharmacology
Peptide Fragments/*pharmacology
Peptides/*pharmacology
Receptors, Mating Factor/*metabolism
Saccharomyces cerevisiae/*metabolism
Saccharomyces cerevisiae Proteins/*metabolism
Binding Sites ; Binding, Competitive ; Cross-Linking Reagents ; Humans ; Immunoblotting ; Ligands ; Mating Factor ; Membrane Proteins/genetics ; Membrane Proteins/metabolism ; Protein Binding ; Receptors, Mating Factor/genetics ; Saccharomyces cerevisiae/drug effects ; Saccharomyces cerevisiae/growth & development ; Saccharomyces cerevisiae Proteins/genetics ; Signal Transduction/drug effects ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Czasopismo naukowe
Tytuł :
Structural characterization of triple transmembrane domain containing fragments of a yeast G protein-coupled receptor in an organic : aqueous environment by solution-state NMR spectroscopy.
Autorzy :
Fracchiolla KE; Department of Chemistry, The College of Staten Island, City University of New York, Staten Island, NY, 10314, USA; Department of Biochemistry, The Graduate Center, City University of New York, New York, NY, 10016, USA.
Cohen LS
Arshava B
Poms M
Zerbe O
Becker JM
Naider F
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Źródło :
Journal of peptide science : an official publication of the European Peptide Society [J Pept Sci] 2015 Mar; Vol. 21 (3), pp. 212-22. Date of Electronic Publication: 2015 Feb 02.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
MeSH Terms :
Receptors, Mating Factor/*chemistry
Saccharomyces cerevisiae Proteins/*chemistry
Trifluoroethanol/*chemistry
Water/*chemistry
Escherichia coli/genetics ; Escherichia coli/metabolism ; Gene Expression ; Isotope Labeling ; Nuclear Magnetic Resonance, Biomolecular ; Protein Structure, Secondary ; Protein Structure, Tertiary ; Receptors, Mating Factor/genetics ; Recombinant Proteins/chemistry ; Recombinant Proteins/genetics ; Saccharomyces cerevisiae/chemistry ; Saccharomyces cerevisiae Proteins/genetics ; Thermodynamics
Czasopismo naukowe
Tytuł :
A Novel Screening Approach for Optimal and Functional Fusion of T4 Lysozyme in GPCRs.
Autorzy :
Mathew E; Department of Biochemistry and Biophysics, P.O. Box 712, University of Rochester School of Medicine and Dentistry, Rochester, New York, USA.
Dumont ME; Department of Biochemistry and Biophysics, P.O. Box 712, University of Rochester School of Medicine and Dentistry, Rochester, New York, USA. Electronic address: .
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Źródło :
Methods in enzymology [Methods Enzymol] 2015; Vol. 557, pp. 27-43. Date of Electronic Publication: 2015 Mar 24.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural
MeSH Terms :
Bacteriophage T4/*genetics
Muramidase/*genetics
Receptors, G-Protein-Coupled/*genetics
Receptors, Mating Factor/*genetics
Saccharomyces cerevisiae/*genetics
Viral Proteins/*genetics
Bacteriophage T4/chemistry ; Gene Expression ; Muramidase/chemistry ; Plasmids/genetics ; Protein Conformation ; Receptors, G-Protein-Coupled/chemistry ; Receptors, Mating Factor/chemistry ; Recombinant Fusion Proteins/chemistry ; Recombinant Fusion Proteins/genetics ; Saccharomyces cerevisiae/chemistry ; Solubility ; Viral Proteins/chemistry
Czasopismo naukowe

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