Temat: "Recombinant Proteins" - OpacWWW

Skocz do pozycji: 1.

Tytuł:: Modifications of the 5' region of the CASPON tag's mRNA further enhance soluble recombinant protein production in Escherichia coli.
Autorzy:: Köppl C; Austrian Centre of Industrial Biotechnology, Muthgasse 18, Vienna, 1190, Austria.; Department of Biotechnology, Institute of Bioprocess Science and Engineering, University of Natural Resources and Life Sciences, Muthgasse 18, Vienna, 1190, Austria.
Buchinger W; Biopharma Austria, Development Operations, Boehringer Ingelheim Regional Center Vienna GmbH & Co KG, Dr.-Boehringer-Gasse 5-11, Vienna, A-1121, Austria.
Striedner G; Austrian Centre of Industrial Biotechnology, Muthgasse 18, Vienna, 1190, Austria.; Department of Biotechnology, Institute of Bioprocess Science and Engineering, University of Natural Resources and Life Sciences, Muthgasse 18, Vienna, 1190, Austria.
Cserjan-Puschmann M; Austrian Centre of Industrial Biotechnology, Muthgasse 18, Vienna, 1190, Austria. .; Department of Biotechnology, Institute of Bioprocess Science and Engineering, University of Natural Resources and Life Sciences, Muthgasse 18, Vienna, 1190, Austria. .; Pokaż więcej
Źródło:: Microbial cell factories [Microb Cell Fact] 2024 Mar 20; Vol. 23 (1), pp. 86. Date of Electronic Publication: 2024 Mar 20.
Typ publikacji:: Journal Article
MeSH Terms:: Escherichia coli*/genetics
Escherichia coli*/metabolism
Carbon*
RNA, Messenger/genetics ; RNA, Messenger/metabolism ; 5' Untranslated Regions ; Codon ; Recombinant Proteins/genetics ; Recombinant Fusion Proteins/genetics

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Skocz do pozycji: 2.

Tytuł:: A Fusion of Taq DNA Polymerase with the CL7 Protein from Escherichia coli Remarkably Improves DNA Amplification.
Autorzy:: Li Z; State Key Laboratory of Biocatalysis and Enzyme, Engineering Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei Key Laboratory of Industrial Biotechnology, Biology Faculty of Hubei University, Hubei University, Wuhan 430062, China.
Wang Y; State Key Laboratory of Biocatalysis and Enzyme, Engineering Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei Key Laboratory of Industrial Biotechnology, Biology Faculty of Hubei University, Hubei University, Wuhan 430062, China.
Wang X; State Key Laboratory of Biocatalysis and Enzyme, Engineering Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei Key Laboratory of Industrial Biotechnology, Biology Faculty of Hubei University, Hubei University, Wuhan 430062, China.
Niu S; State Key Laboratory of Biocatalysis and Enzyme, Engineering Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei Key Laboratory of Industrial Biotechnology, Biology Faculty of Hubei University, Hubei University, Wuhan 430062, China.
Su Z; State Key Laboratory of Biocatalysis and Enzyme, Engineering Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei Key Laboratory of Industrial Biotechnology, Biology Faculty of Hubei University, Hubei University, Wuhan 430062, China.
Wang F; State Key Laboratory of Biocatalysis and Enzyme, Engineering Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei Key Laboratory of Industrial Biotechnology, Biology Faculty of Hubei University, Hubei University, Wuhan 430062, China.
Ni J; State Key Laboratory of Biocatalysis and Enzyme, Engineering Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei Key Laboratory of Industrial Biotechnology, Biology Faculty of Hubei University, Hubei University, Wuhan 430062, China.
Gong Y; State Key Laboratory of Biocatalysis and Enzyme, Engineering Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei Key Laboratory of Industrial Biotechnology, Biology Faculty of Hubei University, Hubei University, Wuhan 430062, China.; Hubei Biopesticide Engineering Research Center, Hubei Academy of Agricultural Sciences, Biopesticide Branch of Hubei Innovation Centre of Agricultural Science and Technology, Wuhan 430064, China.
Rao B; State Key Laboratory of Biocatalysis and Enzyme, Engineering Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei Key Laboratory of Industrial Biotechnology, Biology Faculty of Hubei University, Hubei University, Wuhan 430062, China.; Hubei Biopesticide Engineering Research Center, Hubei Academy of Agricultural Sciences, Biopesticide Branch of Hubei Innovation Centre of Agricultural Science and Technology, Wuhan 430064, China.; Pokaż więcej
Źródło:: Molecules (Basel, Switzerland) [Molecules] 2024 Mar 04; Vol. 29 (5). Date of Electronic Publication: 2024 Mar 04.
Typ publikacji:: Journal Article
MeSH Terms:: Escherichia coli*/metabolism
Nucleic Acid Amplification Techniques*
Humans ; Taq Polymerase/metabolism ; Polymerase Chain Reaction/methods ; DNA/metabolism ; Recombinant Proteins/metabolism

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Skocz do pozycji: 3.

Tytuł:: Scalable production of recombinant three-finger proteins: from inclusion bodies to high quality molecular probes.
Autorzy:: Xu J; Molecular and Computational Biology, Department of Biological Sciences, University of Southern California, Los Angeles, CA, 90089, USA. .
Lei X; Molecular and Computational Biology, Department of Biological Sciences, University of Southern California, Los Angeles, CA, 90089, USA.
Li A; Molecular and Computational Biology, Department of Biological Sciences, University of Southern California, Los Angeles, CA, 90089, USA.
Li J; Institute of Clinical Medicine, The First Affiliated Hospital of University of South China, Hengyang, Hunan, 421001, People's Republic of China.
Li S; Molecular and Computational Biology, Department of Biological Sciences, University of Southern California, Los Angeles, CA, 90089, USA.
Chen L; Molecular and Computational Biology, Department of Biological Sciences, University of Southern California, Los Angeles, CA, 90089, USA. .; Pokaż więcej
Źródło:: Microbial cell factories [Microb Cell Fact] 2024 Feb 12; Vol. 23 (1), pp. 48. Date of Electronic Publication: 2024 Feb 12.
Typ publikacji:: Journal Article
MeSH Terms:: Escherichia coli*/metabolism
Inclusion Bodies*/metabolism
Recombinant Proteins ; Disulfides/metabolism

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Skocz do pozycji: 4.

Tytuł:: Analyzing the correlation between protein expression and sequence-related features of mRNA and protein in Escherichia coli K-12 MG1655 model.
Autorzy:: Truong NHM; Center for Bioscience and Biotechnology, University of Science, Ho Chi Minh City, Vietnam.; Vietnam National University, Ho Chi Minh City, Vietnam.
T Vo N; Center for Bioscience and Biotechnology, University of Science, Ho Chi Minh City, Vietnam.; Vietnam National University, Ho Chi Minh City, Vietnam.; Laboratory of Molecular Biotechnology, University of Science, Ho Chi Minh City, Vietnam.
Nguyen BT; Vietnam National University, Ho Chi Minh City, Vietnam.; Department of Computer Science, University of Science, Ho Chi Minh City, Vietnam.
Huynh ST; Vietnam National University, Ho Chi Minh City, Vietnam.; Department of Computer Science, University of Science, Ho Chi Minh City, Vietnam.
Nguyen HD; Center for Bioscience and Biotechnology, University of Science, Ho Chi Minh City, Vietnam.; Vietnam National University, Ho Chi Minh City, Vietnam.; Pokaż więcej
Źródło:: PloS one [PLoS One] 2024 Feb 07; Vol. 19 (2), pp. e0288526. Date of Electronic Publication: 2024 Feb 07 (Print Publication: 2024).
Typ publikacji:: Journal Article
MeSH Terms:: Escherichia coli*/genetics
Escherichia coli*/metabolism
Escherichia coli K12*/genetics
Escherichia coli K12*/metabolism
RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Genome ; Recombinant Proteins/metabolism ; Protein Biosynthesis/genetics

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Skocz do pozycji: 5.

Tytuł:: RNA-seq reveals multifaceted gene expression response to Fab production in Escherichia coli fed-batch processes with particular focus on ribosome stalling.
Autorzy:: Vazulka S; Christian Doppler Laboratory for Production of Next-Level Biopharmaceuticals in E. Coli, Department of Biotechnology, University of Natural Resources and Life Sciences, Muthgasse 18, 1190, Vienna, Austria.
Schiavinato M; Department of Biotechnology, Institute of Computational Biology, University of Natural Resources and Life Sciences, Muthgasse 18, 1190, Vienna, Austria.
Tauer C; Christian Doppler Laboratory for Production of Next-Level Biopharmaceuticals in E. Coli, Department of Biotechnology, University of Natural Resources and Life Sciences, Muthgasse 18, 1190, Vienna, Austria.
Wagenknecht M; Boehringer Ingelheim RCV, GmbH & Co KG, Dr.-Boehringer-Gasse 5-11, A-1120, Vienna, Austria.
Cserjan-Puschmann M; Christian Doppler Laboratory for Production of Next-Level Biopharmaceuticals in E. Coli, Department of Biotechnology, University of Natural Resources and Life Sciences, Muthgasse 18, 1190, Vienna, Austria. .
Striedner G; Christian Doppler Laboratory for Production of Next-Level Biopharmaceuticals in E. Coli, Department of Biotechnology, University of Natural Resources and Life Sciences, Muthgasse 18, 1190, Vienna, Austria.; Pokaż więcej
Źródło:: Microbial cell factories [Microb Cell Fact] 2024 Jan 05; Vol. 23 (1), pp. 14. Date of Electronic Publication: 2024 Jan 05.
Typ publikacji:: Journal Article
MeSH Terms:: Escherichia coli*/genetics
RNA-Seq ; Sequence Analysis, RNA ; Recombinant Proteins ; Gene Expression

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Skocz do pozycji: 6.

Tytuł:: Human super antibody to viral RNA-dependent RNA polymerase produced by a modified Sortase self-cleave-bacteria surface display system.
Autorzy:: Glab-Ampai K; Center of Research Excellence in Therapeutic Proteins and Antibody Engineering, Department of Parasitology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, 10700, Thailand.
Mahasongkram K; Center of Research Excellence in Therapeutic Proteins and Antibody Engineering, Department of Parasitology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, 10700, Thailand.
Chulanetra M; Center of Research Excellence in Therapeutic Proteins and Antibody Engineering, Department of Parasitology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, 10700, Thailand.
Saenlom T; Center of Research Excellence in Therapeutic Proteins and Antibody Engineering, Department of Parasitology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, 10700, Thailand.
Thueng-In K; School of Pathology, Translational Medicine Program, Institute of Medicine, Suranaree University of Technology, Nakhon Ratchasima, 30000, Thailand.
Sookrung N; Center of Research Excellence in Therapeutic Proteins and Antibody Engineering, Department of Parasitology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, 10700, Thailand.; Biomedical Research Incubation Unit, Department of Research, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, 10700, Thailand.
Chaicumpa W; Center of Research Excellence in Therapeutic Proteins and Antibody Engineering, Department of Parasitology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, 10700, Thailand. .; Pokaż więcej
Źródło:: Microbial cell factories [Microb Cell Fact] 2023 Dec 18; Vol. 22 (1), pp. 260. Date of Electronic Publication: 2023 Dec 18.
Typ publikacji:: Journal Article
MeSH Terms:: Escherichia coli*/metabolism
Single-Chain Antibodies*/genetics
Humans ; RNA-Dependent RNA Polymerase/genetics ; RNA-Dependent RNA Polymerase/metabolism ; Recombinant Proteins ; Membrane Proteins

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Skocz do pozycji: 7.

Tytuł:: Enhanced production of a recombinant xylanase (XT6): optimization of production and purification, and scaled-up batch fermentation in a stirred tank bioreactor.
Autorzy:: Dhaver P; Discipline of Microbiology, School of Life Sciences, University of KwaZulu-Natal, Westville Campus, Durban, 4000, South Africa.
Sithole T; Enzyme Science Programme (ESP), Department of Biochemistry and Microbiology, Rhodes University, Makhanda (Grahamstown), 6140, Eastern Cape, South Africa.
Pletschke B; Enzyme Science Programme (ESP), Department of Biochemistry and Microbiology, Rhodes University, Makhanda (Grahamstown), 6140, Eastern Cape, South Africa. .
Sithole B; Biorefinery Industry Development Facility, Council for Scientific and Industrial Research, Durban, 4000, South Africa.; Discipline of Chemical Engineering, University of KwaZulu-Natal, Durban, 4000, South Africa.
Govinden R; Discipline of Microbiology, School of Life Sciences, University of KwaZulu-Natal, Westville Campus, Durban, 4000, South Africa.; Pokaż więcej
Źródło:: Scientific reports [Sci Rep] 2023 Nov 28; Vol. 13 (1), pp. 20895. Date of Electronic Publication: 2023 Nov 28.
Typ publikacji:: Journal Article
MeSH Terms:: Bioreactors*
Escherichia coli*/genetics
Escherichia coli*/metabolism
Fermentation ; Recombinant Proteins/metabolism ; Endo-1,4-beta Xylanases/metabolism

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Skocz do pozycji: 8.

Tytuł:: Serodiagnosis of human brucellosis by an indirect ELISA test using recombinant outer membrane protein 19 kDa (rOMP19) as an antigen.
Autorzy:: Golchin M; Department of Pathobiology, School of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.
Mollayi S; School of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.
Mohammadi E; Department of Pathobiology, School of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.
Eskandarzade N; Department of Basic Sciences, School of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, 7616914111, Iran. .; Pokaż więcej
Źródło:: BMC biotechnology [BMC Biotechnol] 2023 Oct 24; Vol. 23 (1), pp. 46. Date of Electronic Publication: 2023 Oct 24.
Typ publikacji:: Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:: Escherichia coli*/genetics
Brucellosis*/diagnosis
Humans ; Rose Bengal ; Enzyme-Linked Immunosorbent Assay/methods ; Recombinant Proteins/genetics ; Serologic Tests/methods ; Antibodies, Bacterial ; Sensitivity and Specificity

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Skocz do pozycji: 9.

Tytuł:: Development of an Anti-HER2 Single-Chain Variable Antibody Fragment Construct for High-Yield Soluble Expression in Escherichia coli and One-Step Chromatographic Purification.
Autorzy:: Byun KT; Department of Biotechnology, Research Institute (RIBHS), College of Biomedical and Health Science, Konkuk University, Chungju 27478, Republic of Korea.
Kim B; Department of Biotechnology, Research Institute (RIBHS), College of Biomedical and Health Science, Konkuk University, Chungju 27478, Republic of Korea.
Cho J; Department of Biotechnology, Research Institute (RIBHS), College of Biomedical and Health Science, Konkuk University, Chungju 27478, Republic of Korea.
Lee I; Department of Biotechnology, Research Institute (RIBHS), College of Biomedical and Health Science, Konkuk University, Chungju 27478, Republic of Korea.
Lee MG; Konkukbio Inc., Konkuk University, Chungju 27478, Republic of Korea.
Park D; Department of Biology Education, Korea National University of Education, Cheongju 28173, Republic of Korea.
Kang TB; Department of Biotechnology, Research Institute (RIBHS), College of Biomedical and Health Science, Konkuk University, Chungju 27478, Republic of Korea.; BK21 Project Team, Department of Applied Life Science, Graduate School, Konkuk University, Chungju 27478, Republic of Korea.
Won HS; Department of Biotechnology, Research Institute (RIBHS), College of Biomedical and Health Science, Konkuk University, Chungju 27478, Republic of Korea.; BK21 Project Team, Department of Applied Life Science, Graduate School, Konkuk University, Chungju 27478, Republic of Korea.
Kim CG; Department of Biotechnology, Research Institute (RIBHS), College of Biomedical and Health Science, Konkuk University, Chungju 27478, Republic of Korea.; Pokaż więcej
Źródło:: Biomolecules [Biomolecules] 2023 Oct 11; Vol. 13 (10). Date of Electronic Publication: 2023 Oct 11.
Typ publikacji:: Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:: Escherichia coli*/genetics
Escherichia coli*/metabolism
Single-Chain Antibodies*/chemistry
Recombinant Proteins/metabolism ; Chromatography, Affinity ; Maltose-Binding Proteins/genetics

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Skocz do pozycji: 10.

Tytuł:: Outer membrane protein N expressed in Gram-negative bacterial strain of Escherichia coli BL21 (DE3) Omp8 Rosetta strains under osmoregulation by salts, sugars, and pHs.
Autorzy:: Chumjan W; Department of Chemistry, Rajamangala University of Technology Isan Khon Kaen Campus, Khon Kaen, Thailand.
Sangchalee A; Department of Chemistry, Rajamangala University of Technology Isan Khon Kaen Campus, Khon Kaen, Thailand.
Somwang C; Department of Chemistry, Rajamangala University of Technology Isan Khon Kaen Campus, Khon Kaen, Thailand.
Mookda N; Department of Chemistry, Rajamangala University of Technology Isan Khon Kaen Campus, Khon Kaen, Thailand.
Yaikeaw S; Department of Chemistry, Rajamangala University of Technology Isan Khon Kaen Campus, Khon Kaen, Thailand.
Somsakeesit LO; Department of Chemistry, Rajamangala University of Technology Isan Khon Kaen Campus, Khon Kaen, Thailand.; Pokaż więcej
Źródło:: PloS one [PLoS One] 2023 Aug 03; Vol. 18 (8), pp. e0288096. Date of Electronic Publication: 2023 Aug 03 (Print Publication: 2023).
Typ publikacji:: Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:: Escherichia coli*/genetics
Escherichia coli*/metabolism
Salts*/metabolism
Osmoregulation ; Sugars/metabolism ; Isopropyl Thiogalactoside/pharmacology ; Recombinant Proteins/metabolism ; Membrane Proteins/metabolism

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Skocz do pozycji: 11.

Tytuł:: Extracellular production of Ulp1 403-621 in leaky E. coli and its application in antimicrobial peptide production.
Autorzy:: Fu L; State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, People's Republic of China.
Sun M; State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, People's Republic of China.
Wen W; State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, People's Republic of China.
Dong N; State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, People's Republic of China. .
Li D; State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, People's Republic of China. .; Pokaż więcej
Źródło:: Applied microbiology and biotechnology [Appl Microbiol Biotechnol] 2022 Dec; Vol. 106 (23), pp. 7805-7817. Date of Electronic Publication: 2022 Oct 19.
Typ publikacji:: Journal Article
MeSH Terms:: Antimicrobial Peptides*
Escherichia coli*/metabolism
Recombinant Proteins*/biosynthesis
Recombinant Proteins*/genetics
Endopeptidases/genetics ; Endopeptidases/metabolism ; Glycine/metabolism ; Peptide Hydrolases/metabolism ; Ubiquitins/metabolism

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Skocz do pozycji: 12.

Tytuł:: Effects of Signal Peptide and Chaperone Co-Expression on Heterologous Protein Production in Escherichia coli .
Autorzy:: Jomrit J; School of Pharmacy, Walailak University, Nakhon Si Thammarat 80160, Thailand.
Suhardi S; Department of Animal Science, Faculty of Agriculture, Mulawarman University, Samarinda 75123, Indonesia.
Summpunn P; Food Technology and Innovation Research Center of Excellence, School of Agricultural Technology and Food industry, Walailak University, Nakhon Si Thammarat 80160, Thailand.; Pokaż więcej
Źródło:: Molecules (Basel, Switzerland) [Molecules] 2023 Jul 23; Vol. 28 (14). Date of Electronic Publication: 2023 Jul 23.
Typ publikacji:: Journal Article
MeSH Terms:: Escherichia coli*/genetics
Escherichia coli*/metabolism
Escherichia coli Proteins*/metabolism
Humans ; beta-Mannosidase/metabolism ; Molecular Chaperones/genetics ; Molecular Chaperones/metabolism ; Recombinant Proteins/metabolism

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Skocz do pozycji: 13.

Tytuł:: Purification of recombinant bacterial collagens containing structural perturbations.
Autorzy:: Gahlawat S; Department of Biomedical Engineering, Rutgers, The State University of New Jersey, Piscataway, NJ, United States of America.
Nanda V; Department of Biochemistry and Molecular Biology, Robert Wood Johnson Medical School, Rutgers, The State University of New Jersey, Piscataway, NJ, United States of America.; Center for Advanced Biotechnology and Medicine, Rutgers, The State University of New Jersey, Piscataway, NJ, United States of America.
Shreiber DI; Department of Biomedical Engineering, Rutgers, The State University of New Jersey, Piscataway, NJ, United States of America.; Pokaż więcej
Źródło:: PloS one [PLoS One] 2023 May 17; Vol. 18 (5), pp. e0285864. Date of Electronic Publication: 2023 May 17 (Print Publication: 2023).
Typ publikacji:: Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:: Escherichia coli*/genetics
Escherichia coli*/metabolism
Collagen*/metabolism
Humans ; Trypsin ; Recombinant Proteins/genetics ; Bacterial Proteins/metabolism ; Biocompatible Materials ; Recombinant Fusion Proteins

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Skocz do pozycji: 14.

Tytuł:: Fundamental insights in early-stage inclusion body formation.
Autorzy:: Kopp J; Research Division Integrated Bioprocess Development, Institute of Chemical, Environmental and Bioscience, Vienna, Austria.
Bayer B; Department of Biotechnology, University of Natural Resources and Life Sciences, Vienna, Austria.; Novasign GmbH, Vienna, Austria.
Slouka C; Research Division Integrated Bioprocess Development, Institute of Chemical, Environmental and Bioscience, Vienna, Austria.
Striedner G; Department of Biotechnology, University of Natural Resources and Life Sciences, Vienna, Austria.; Novasign GmbH, Vienna, Austria.
Dürkop M; Department of Biotechnology, University of Natural Resources and Life Sciences, Vienna, Austria.; Novasign GmbH, Vienna, Austria.
Spadiut O; Research Division Integrated Bioprocess Development, Institute of Chemical, Environmental and Bioscience, Vienna, Austria.; Pokaż więcej
Źródło:: Microbial biotechnology [Microb Biotechnol] 2023 May; Vol. 16 (5), pp. 893-900. Date of Electronic Publication: 2022 Jul 13.
Typ publikacji:: Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:: Protein Aggregates*
Escherichia coli*/genetics
Escherichia coli*/metabolism
Inclusion Bodies/metabolism ; Recombinant Proteins/metabolism

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Skocz do pozycji: 15.

Tytuł:: Effects of adding poly-histidine tag on stability, antimicrobial activity and safety of recombinant buforin I expressed in periplasmic space of Escherichia coli.
Autorzy:: Roshanak S; Department of Food Science and Technology, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.
Yarabbi H; Department of Food Science and Technology, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.
Shahidi F; Department of Food Science and Technology, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.
Tabatabaei Yazdi F; Department of Food Science and Technology, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.
Movaffagh J; Department of Pharmaceutical Nanotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
Javadmanesh A; Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Azadi Square, Mashhad, 9177948974, Razavi Khorasan Province, Iran. .; Industrial Biotechnology Research Group, Research Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran. .; Pokaż więcej
Źródło:: Scientific reports [Sci Rep] 2023 Apr 04; Vol. 13 (1), pp. 5508. Date of Electronic Publication: 2023 Apr 04.
Typ publikacji:: Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:: Escherichia coli*/metabolism
Anti-Infective Agents*/pharmacology
Animals ; Periplasm/metabolism ; Antimicrobial Cationic Peptides/genetics ; Antimicrobial Cationic Peptides/pharmacology ; Antimicrobial Cationic Peptides/metabolism ; Tomography, X-Ray Computed ; Recombinant Proteins/genetics ; Recombinant Proteins/pharmacology

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Skocz do pozycji: 16.

Tytuł:: Chaperone assisted recombinant expression of a mycobacterial aminoacylase in Vibrio natriegens and Escherichia coli capable of N-lauroyl-L-amino acid synthesis.
Autorzy:: Haeger G; Institute of Nano- and Biotechnologies, Aachen University of Applied Sciences, 52428, Jülich, Germany.
Wirges J; Institute of Nano- and Biotechnologies, Aachen University of Applied Sciences, 52428, Jülich, Germany.
Tanzmann N; Institute of Nano- and Biotechnologies, Aachen University of Applied Sciences, 52428, Jülich, Germany.
Oyen S; Institute of Nano- and Biotechnologies, Aachen University of Applied Sciences, 52428, Jülich, Germany.
Jolmes T; TH Köln - Campus Leverkusen, 51379, Leverkusen, Germany.
Jaeger KE; Institute of Molecular Enzyme Technology, Heinrich Heine University Düsseldorf, 52425, Jülich, Germany.; Institute of Bio- and Geosciences IBG-1: Biotechnology, Forschungszentrum Jülich GmbH, Jülich, 52425, Germany.
Schörken U; TH Köln - Campus Leverkusen, 51379, Leverkusen, Germany.
Bongaerts J; Institute of Nano- and Biotechnologies, Aachen University of Applied Sciences, 52428, Jülich, Germany.
Siegert P; Institute of Nano- and Biotechnologies, Aachen University of Applied Sciences, 52428, Jülich, Germany. .; Pokaż więcej
Źródło:: Microbial cell factories [Microb Cell Fact] 2023 Apr 21; Vol. 22 (1), pp. 77. Date of Electronic Publication: 2023 Apr 21.
Typ publikacji:: Journal Article
MeSH Terms:: Escherichia coli*/genetics
Escherichia coli*/metabolism
Amino Acids*/metabolism
Recombinant Proteins/genetics ; Methionine
SCR Organism:: Vibrio natriegens

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Skocz do pozycji: 17.

Tytuł:: Simplified cloning and isolation of peptides from "sandwiched" SUMO-peptide-intein fusion proteins.
Autorzy:: Lamer T; Department of Chemistry, University of Alberta, Edmonton, AB, T6G 2G2, Canada.
Vederas JC; Department of Chemistry, University of Alberta, Edmonton, AB, T6G 2G2, Canada. .; Pokaż więcej
Źródło:: BMC biotechnology [BMC Biotechnol] 2023 Apr 05; Vol. 23 (1), pp. 11. Date of Electronic Publication: 2023 Apr 05.
Typ publikacji:: Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:: Inteins*
Escherichia coli*/genetics
Recombinant Fusion Proteins/genetics ; Small Ubiquitin-Related Modifier Proteins/genetics ; Peptides/genetics ; Recombinant Proteins/genetics ; Cloning, Molecular

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Skocz do pozycji: 18.

Tytuł:: Cell-Free Expression of a Therapeutic Protein Serratiopeptidase.
Autorzy:: Meng Y; School of Physical Science and Technology, ShanghaiTech University, Shanghai 201210, China.
Yang M; Clinical Pathology Center, The First Affiliated Hospital of Anhui Medical University, Hefei 230012, China.; Anhui Public Health Clinical Center, Hefei 230012, China.
Liu W; School of Physical Science and Technology, ShanghaiTech University, Shanghai 201210, China.
Li J; School of Physical Science and Technology, ShanghaiTech University, Shanghai 201210, China.; Pokaż więcej
Źródło:: Molecules (Basel, Switzerland) [Molecules] 2023 Mar 31; Vol. 28 (7). Date of Electronic Publication: 2023 Mar 31.
Typ publikacji:: Journal Article
MeSH Terms:: Escherichia coli*/genetics
Escherichia coli*/metabolism
Protein Biosynthesis*
Humans ; Cell Extracts ; Recombinant Proteins/metabolism ; Cell-Free System/metabolism

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Skocz do pozycji: 19.

Tytuł:: Acyl carrier protein tag can enhance tobacco etch virus protease stability and promote its covalent immobilisation.
Autorzy:: Li X; College of Life Sciences, South China Agricultural University, Guangzhou, 510642, People's Republic of China.; Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, Guangzhou, 510642, People's Republic of China.
Huang J; College of Life Sciences, South China Agricultural University, Guangzhou, 510642, People's Republic of China.; Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, Guangzhou, 510642, People's Republic of China.
Zhou J; College of Life Sciences, South China Agricultural University, Guangzhou, 510642, People's Republic of China.; Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, Guangzhou, 510642, People's Republic of China.
Sun C; College of Life Sciences, South China Agricultural University, Guangzhou, 510642, People's Republic of China.; Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, Guangzhou, 510642, People's Republic of China.
Zheng Y; College of Life Sciences, South China Agricultural University, Guangzhou, 510642, People's Republic of China.; Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, Guangzhou, 510642, People's Republic of China.
Wang Y; College of Life Sciences, South China Agricultural University, Guangzhou, 510642, People's Republic of China.; Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, Guangzhou, 510642, People's Republic of China.
Zhu J; College of Life Sciences, South China Agricultural University, Guangzhou, 510642, People's Republic of China.; Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, Guangzhou, 510642, People's Republic of China.
Wang S; College of Life Sciences, South China Agricultural University, Guangzhou, 510642, People's Republic of China. .; Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, Guangzhou, 510642, People's Republic of China. .; Pokaż więcej
Źródło:: Applied microbiology and biotechnology [Appl Microbiol Biotechnol] 2023 Mar; Vol. 107 (5-6), pp. 1697-1705. Date of Electronic Publication: 2023 Feb 10.
Typ publikacji:: Journal Article
MeSH Terms:: Escherichia coli*/metabolism
Acyl Carrier Protein*
Recombinant Fusion Proteins/metabolism ; Recombinant Proteins/metabolism ; Endopeptidases/metabolism

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Skocz do pozycji: 20.

Tytuł:: Omics-guided bacterial engineering of Escherichia coli ER2566 for recombinant protein expression.
Autorzy:: Zhou L; State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, School of Life Sciences, Xiamen University, Xiamen, 361102, Fujian, China.; National Institute of Diagnostics and Vaccine Development in Infectious Disease, Xiamen University, Xiamen, 361102, Fujian, China.
Ma Y; State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, School of Life Sciences, Xiamen University, Xiamen, 361102, Fujian, China.; National Institute of Diagnostics and Vaccine Development in Infectious Disease, Xiamen University, Xiamen, 361102, Fujian, China.
Wang K; College of Medicine, The First Affiliated Hospital, Zhejiang University, Hangzhou, 310003, Zhejiang, China.
Chen T; State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, School of Life Sciences, Xiamen University, Xiamen, 361102, Fujian, China.; National Institute of Diagnostics and Vaccine Development in Infectious Disease, Xiamen University, Xiamen, 361102, Fujian, China.
Huang Y; State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, School of Life Sciences, Xiamen University, Xiamen, 361102, Fujian, China.; National Institute of Diagnostics and Vaccine Development in Infectious Disease, Xiamen University, Xiamen, 361102, Fujian, China.
Liu L; State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, School of Life Sciences, Xiamen University, Xiamen, 361102, Fujian, China.; National Institute of Diagnostics and Vaccine Development in Infectious Disease, Xiamen University, Xiamen, 361102, Fujian, China.
Li Y; State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, School of Life Sciences, Xiamen University, Xiamen, 361102, Fujian, China.; National Institute of Diagnostics and Vaccine Development in Infectious Disease, Xiamen University, Xiamen, 361102, Fujian, China.
Sun J; State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, School of Life Sciences, Xiamen University, Xiamen, 361102, Fujian, China.; National Institute of Diagnostics and Vaccine Development in Infectious Disease, Xiamen University, Xiamen, 361102, Fujian, China.
Hu Y; State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, School of Life Sciences, Xiamen University, Xiamen, 361102, Fujian, China.; National Institute of Diagnostics and Vaccine Development in Infectious Disease, Xiamen University, Xiamen, 361102, Fujian, China.
Li T; State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, School of Life Sciences, Xiamen University, Xiamen, 361102, Fujian, China.; National Institute of Diagnostics and Vaccine Development in Infectious Disease, Xiamen University, Xiamen, 361102, Fujian, China.
Kong Z; State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, School of Life Sciences, Xiamen University, Xiamen, 361102, Fujian, China.; National Institute of Diagnostics and Vaccine Development in Infectious Disease, Xiamen University, Xiamen, 361102, Fujian, China.
Wang Y; State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, School of Life Sciences, Xiamen University, Xiamen, 361102, Fujian, China.; National Institute of Diagnostics and Vaccine Development in Infectious Disease, Xiamen University, Xiamen, 361102, Fujian, China.
Zheng Q; State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, School of Life Sciences, Xiamen University, Xiamen, 361102, Fujian, China.; National Institute of Diagnostics and Vaccine Development in Infectious Disease, Xiamen University, Xiamen, 361102, Fujian, China.
Zhao Q; State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, School of Life Sciences, Xiamen University, Xiamen, 361102, Fujian, China.; National Institute of Diagnostics and Vaccine Development in Infectious Disease, Xiamen University, Xiamen, 361102, Fujian, China.
Zhang J; State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, School of Life Sciences, Xiamen University, Xiamen, 361102, Fujian, China.; National Institute of Diagnostics and Vaccine Development in Infectious Disease, Xiamen University, Xiamen, 361102, Fujian, China.
Gu Y; State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, School of Life Sciences, Xiamen University, Xiamen, 361102, Fujian, China.; National Institute of Diagnostics and Vaccine Development in Infectious Disease, Xiamen University, Xiamen, 361102, Fujian, China.
Yu H; State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, School of Life Sciences, Xiamen University, Xiamen, 361102, Fujian, China. .; National Institute of Diagnostics and Vaccine Development in Infectious Disease, Xiamen University, Xiamen, 361102, Fujian, China. .
Xia N; State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, School of Life Sciences, Xiamen University, Xiamen, 361102, Fujian, China.; National Institute of Diagnostics and Vaccine Development in Infectious Disease, Xiamen University, Xiamen, 361102, Fujian, China.
Li S; State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, School of Life Sciences, Xiamen University, Xiamen, 361102, Fujian, China. .; National Institute of Diagnostics and Vaccine Development in Infectious Disease, Xiamen University, Xiamen, 361102, Fujian, China. .; Pokaż więcej
Źródło:: Applied microbiology and biotechnology [Appl Microbiol Biotechnol] 2023 Feb; Vol. 107 (2-3), pp. 853-865. Date of Electronic Publication: 2022 Dec 21.
Typ publikacji:: Journal Article
MeSH Terms:: Escherichia coli*/genetics
Escherichia coli*/metabolism
Escherichia coli Proteins*/genetics
Escherichia coli Proteins*/metabolism
Recombinant Proteins/metabolism ; Transcriptome ; Uridine Diphosphate/metabolism ; Metabolic Engineering

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