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Tytuł :
[Monitoring the spread of the SARS-CoV-2 (Coronaviridae: Coronavirinae: Betacoronavirus; Sarbecovirus) variants in the Moscow region using targeted high-throughput sequencing].
Autorzy :
Borisova NI; FSBI «Central Research Institute for Epidemiology» of the Federal Service for Supervision of Consumer Rights Protection and Human Welfare (Rospotrebnadzor).
Kotov IA; FSBI «Central Research Institute for Epidemiology» of the Federal Service for Supervision of Consumer Rights Protection and Human Welfare (Rospotrebnadzor); FSAEI HE «Moscow Institute of Physics and Technology (National Research University)».
Kolesnikov AA; FSBI «Central Research Institute for Epidemiology» of the Federal Service for Supervision of Consumer Rights Protection and Human Welfare (Rospotrebnadzor).
Kaptelova VV; FSBI «Central Research Institute for Epidemiology» of the Federal Service for Supervision of Consumer Rights Protection and Human Welfare (Rospotrebnadzor).
Speranskaya AS; FSBI «Central Research Institute for Epidemiology» of the Federal Service for Supervision of Consumer Rights Protection and Human Welfare (Rospotrebnadzor).
Kondrasheva LY; FSBI «Central Research Institute for Epidemiology» of the Federal Service for Supervision of Consumer Rights Protection and Human Welfare (Rospotrebnadzor).
Tivanova EV; FSBI «Central Research Institute for Epidemiology» of the Federal Service for Supervision of Consumer Rights Protection and Human Welfare (Rospotrebnadzor).
Khafizov KF; FSBI «Central Research Institute for Epidemiology» of the Federal Service for Supervision of Consumer Rights Protection and Human Welfare (Rospotrebnadzor).
Akimkin VG; FSBI «Central Research Institute for Epidemiology» of the Federal Service for Supervision of Consumer Rights Protection and Human Welfare (Rospotrebnadzor).
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Źródło :
Voprosy virusologii [Vopr Virusol] 2021 Sep 18; Vol. 66 (4), pp. 269-278. Date of Electronic Publication: 2021 Sep 18.
Typ publikacji :
Journal Article
MeSH Terms :
COVID-19*/diagnosis
COVID-19*/epidemiology
COVID-19*/genetics
COVID-19*/transmission
COVID-19 Nucleic Acid Testing*
High-Throughput Nucleotide Sequencing*
Reverse Transcriptase Polymerase Chain Reaction*
SARS-CoV-2/*genetics
Female ; Humans ; Male ; Moscow/epidemiology
SCR Organism :
SARS-CoV-2 variants
Czasopismo naukowe
Tytuł :
In-house reverse transcriptase polymerase chain reaction for detection of SARS-CoV-2 with increased sensitivity.
Autorzy :
Kalita MJ; Laboratory of Molecular Virology and Oncology, Department of Bioengineering and Technology, Gauhati University, Guwahati, Assam, 781014, India.
Dutta K; Laboratory of Molecular Virology and Oncology, Department of Bioengineering and Technology, Gauhati University, Guwahati, Assam, 781014, India.
Hazarika G; Laboratory of Molecular Virology and Oncology, Department of Bioengineering and Technology, Gauhati University, Guwahati, Assam, 781014, India.
Dutta R; Department of Microbiology, GMCH, Guwahati, Assam, 781032, India.
Kalita S; Laboratory of Molecular Virology and Oncology, Department of Bioengineering and Technology, Gauhati University, Guwahati, Assam, 781014, India.; Department of Medicine, GMCH, Guwahati, Assam, 781032, India.
Das PP; Laboratory of Molecular Virology and Oncology, Department of Bioengineering and Technology, Gauhati University, Guwahati, Assam, 781014, India.; Department of Medicine, GMCH, Guwahati, Assam, 781032, India.
Sarma MP; Department of Biotechnology, Assam Down Town University, Guwahati, Assam, 781068, India.
Banu S; Laboratory of Molecular Virology and Oncology, Department of Bioengineering and Technology, Gauhati University, Guwahati, Assam, 781014, India.
Idris MG; Laboratory of Molecular Virology and Oncology, Department of Bioengineering and Technology, Gauhati University, Guwahati, Assam, 781014, India.
Talukdar AJ; Department of Medicine, GMCH, Guwahati, Assam, 781032, India.
Dutta S; Department of Medicine, GMCH, Guwahati, Assam, 781032, India.
Sharma A; Department of Microbiology, GMCH, Guwahati, Assam, 781032, India. .
Medhi S; Laboratory of Molecular Virology and Oncology, Department of Bioengineering and Technology, Gauhati University, Guwahati, Assam, 781014, India. .
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Źródło :
Scientific reports [Sci Rep] 2021 Sep 09; Vol. 11 (1), pp. 17878. Date of Electronic Publication: 2021 Sep 09.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
COVID-19/*diagnosis
COVID-19 Nucleic Acid Testing/*methods
Nucleic Acid Amplification Techniques/*methods
Reverse Transcriptase Polymerase Chain Reaction/*methods
SARS-CoV-2/*genetics
Humans ; Nucleic Acid Amplification Techniques/economics ; RNA, Viral/genetics ; RNA-Dependent RNA Polymerase/genetics ; Reverse Transcriptase Polymerase Chain Reaction/economics ; Sensitivity and Specificity ; Spike Glycoprotein, Coronavirus/genetics ; Viroporin Proteins/genetics
Czasopismo naukowe
Tytuł :
Performance of RT-PCR on Saliva Specimens Compared With Nasopharyngeal Swabs for the Detection of SARS-CoV-2 in Children: A Prospective Comparative Clinical Trial.
Autorzy :
Fougère Y; From the Pediatric Infectious Diseases and Vaccinology Unit, Department Women-Mother-Child, Lausanne University Hospital, Lausanne, Switzerland.
Schwob JM; Department of Formation, Research and Innovation, Tropical and Travel Medicine Unit, Center for Primary Care and Public Health (Unisanté), Lausanne, Switzerland.
Miauton A; Department of Formation, Research and Innovation, Tropical and Travel Medicine Unit, Center for Primary Care and Public Health (Unisanté), Lausanne, Switzerland.
Hoegger F; Service of Pediatrics, Department Women-Mother-Child, Lausanne University Hospital, Lausanne, Switzerland.
Opota O; Department of Laboratory, Institute of Microbiology, Lausanne University Hospital, Lausanne, Switzerland.
Jaton K; Department of Laboratory, Institute of Microbiology, Lausanne University Hospital, Lausanne, Switzerland.
Brouillet R; Department of Laboratory, Institute of Microbiology, Lausanne University Hospital, Lausanne, Switzerland.
Greub G; Department of Laboratory, Institute of Microbiology, Lausanne University Hospital, Lausanne, Switzerland.; University of Lausanne, Lausanne, Switzerland.
Genton B; Department of Formation, Research and Innovation, Tropical and Travel Medicine Unit, Center for Primary Care and Public Health (Unisanté), Lausanne, Switzerland.; University of Lausanne, Lausanne, Switzerland.
Gehri M; Service of Pediatrics, Department Women-Mother-Child, Lausanne University Hospital, Lausanne, Switzerland.
Taddeo I; Pediatric Emergency Center, Vidy-Med, Lausanne, Switzerland.
D'Acremont V; Department of Formation, Research and Innovation, Tropical and Travel Medicine Unit, Center for Primary Care and Public Health (Unisanté), Lausanne, Switzerland.; University of Lausanne, Lausanne, Switzerland.
Asner SA; From the Pediatric Infectious Diseases and Vaccinology Unit, Department Women-Mother-Child, Lausanne University Hospital, Lausanne, Switzerland.; University of Lausanne, Lausanne, Switzerland.; Infectious Diseases Service, Department of Medicine, Lausanne University Hospital, University of Lausanne, Lausanne, Switzerland.
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Źródło :
The Pediatric infectious disease journal [Pediatr Infect Dis J] 2021 Aug 01; Vol. 40 (8), pp. e300-e304.
Typ publikacji :
Clinical Trial; Comparative Study; Journal Article
MeSH Terms :
COVID-19 Testing*
Reverse Transcriptase Polymerase Chain Reaction*
COVID-19/*diagnosis
COVID-19/*virology
Nasopharynx/*virology
SARS-CoV-2/*isolation & purification
Saliva/*virology
Child ; Child, Preschool ; Diagnostic Tests, Routine ; Female ; Humans ; Infant ; Infant, Newborn ; Male ; Prospective Studies ; SARS-CoV-2/genetics ; Sensitivity and Specificity ; Specimen Handling ; Viral Load
Czasopismo naukowe
Tytuł :
Comparison of RNA- and DNA-based methods for measurable residual disease analysis in NPM1-mutated acute myeloid leukemia.
Autorzy :
Pettersson L; Department of Clinical Sciences, Division of Pathology, Lund University, Skane University Hospital, Lund, Sweden.; Department of Pathology, Halland Hospital Halmstad, Region Halland, Halmstad, Sweden.
Johansson Alm S; Department of Laboratory Medicine, Institute of Biomedicine, Sahlgrenska Academy at University of Gothenburg, Gothenburg, Sweden.
Almstedt A; SciLife Clinical Genomics Gothenburg, Gothenburg, Sweden.
Chen Y; Department of Clinical Sciences, Division of Oncology, Faculty of Medicine, Lund University, Lund, Sweden.
Orrsjö G; Section for Hematology and Coagulation, Sahlgrenska University Hospital, Gothenburg, Sweden.
Shah-Barkhordar G; Department of Clinical Genetics and Genomics, Sahlgrenska University Hospital, Gothenburg, Sweden.
Zhou L; Klinisk Patologi, Region Laboratories, Region Skåne, Lund, Sweden.
Kotarsky H; Klinisk Patologi, Region Laboratories, Region Skåne, Lund, Sweden.
Vidovic K; Department of Clinical Sciences, Division of Pathology, Lund University, Skane University Hospital, Lund, Sweden.
Asp J; Department of Laboratory Medicine, Institute of Biomedicine, Sahlgrenska Academy at University of Gothenburg, Gothenburg, Sweden.; Department of Clinical Chemistry, Sahlgrenska University Hospital, Gothenburg, Sweden.
Lazarevic V; Department of Hematology, Oncology and Radiation Physics, Lund University, Skane University Hospital, Lund, Sweden.
Saal LH; Department of Clinical Sciences, Division of Oncology, Faculty of Medicine, Lund University, Lund, Sweden.; Lund University Cancer Center, Medicon Village, Lund, Sweden.
Fogelstrand L; Department of Laboratory Medicine, Institute of Biomedicine, Sahlgrenska Academy at University of Gothenburg, Gothenburg, Sweden.; Department of Clinical Chemistry, Sahlgrenska University Hospital, Gothenburg, Sweden.
Ehinger M; Department of Clinical Sciences, Division of Pathology, Lund University, Skane University Hospital, Lund, Sweden.; Klinisk Patologi, Region Laboratories, Region Skåne, Lund, Sweden.
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Źródło :
International journal of laboratory hematology [Int J Lab Hematol] 2021 Aug; Vol. 43 (4), pp. 664-674. Date of Electronic Publication: 2021 May 30.
Typ publikacji :
Comparative Study; Journal Article
MeSH Terms :
Mutation*
Real-Time Polymerase Chain Reaction*
Reverse Transcriptase Polymerase Chain Reaction*
DNA, Neoplasm/*blood
Leukemia, Myeloid, Acute/*blood
Nuclear Proteins/*metabolism
RNA, Neoplasm/*blood
Adult ; Aged ; DNA, Neoplasm/genetics ; Female ; Humans ; Leukemia, Myeloid, Acute/genetics ; Male ; Middle Aged ; Neoplasm, Residual/blood ; Nuclear Proteins/genetics ; RNA, Neoplasm/genetics
Czasopismo naukowe
Tytuł :
Validation of real-time RT-PCR for detection of SARS-CoV-2 in the early stages of the COVID-19 outbreak in the Republic of Korea.
Autorzy :
Chung YS; Division of Infectious Disease Diagnosis Control, Honam Regional Centers for Disease Control and Prevention, Korea Diseases Control and Prevention Agency, Gwangju-si, 61947, Republic of Korea.
Lee NJ; Division of Emerging Infectious Diseases, Bureau of Infectious Disease Diagnosis Control, Korea Diseases Control and Prevention Agency, Cheongju-si, 28159, Republic of Korea.
Woo SH; Division of Emerging Infectious Diseases, Bureau of Infectious Disease Diagnosis Control, Korea Diseases Control and Prevention Agency, Cheongju-si, 28159, Republic of Korea.
Kim JM; Division of Emerging Infectious Diseases, Bureau of Infectious Disease Diagnosis Control, Korea Diseases Control and Prevention Agency, Cheongju-si, 28159, Republic of Korea.
Kim HM; Division of Emerging Infectious Diseases, Bureau of Infectious Disease Diagnosis Control, Korea Diseases Control and Prevention Agency, Cheongju-si, 28159, Republic of Korea.
Jo HJ; Division of Emerging Infectious Diseases, Bureau of Infectious Disease Diagnosis Control, Korea Diseases Control and Prevention Agency, Cheongju-si, 28159, Republic of Korea.
Park YE; Division of Laboratory Diagnosis Management, Bureau of Infectious Disease Diagnosis Control, Korea Diseases Control and Prevention Agency, Cheongju-si, 28159, Republic of Korea.
Han MG; Division of Viral Diseases, Bureau of Infectious Disease Diagnosis Control, Korea Diseases Control and Prevention Agency, 187 Osongsaengmyeong2-ro, Osong-eup, Heungdeok-gu, Cheongju-si, 28159, Republic of Korea. .
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Źródło :
Scientific reports [Sci Rep] 2021 Jul 20; Vol. 11 (1), pp. 14817. Date of Electronic Publication: 2021 Jul 20.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't; Validation Study
MeSH Terms :
COVID-19/*epidemiology
COVID-19 Nucleic Acid Testing/*methods
Real-Time Polymerase Chain Reaction/*methods
Reverse Transcriptase Polymerase Chain Reaction/*methods
Animals ; COVID-19/virology ; COVID-19 Nucleic Acid Testing/standards ; Chlorocebus aethiops ; Humans ; Limit of Detection ; Real-Time Polymerase Chain Reaction/standards ; Reproducibility of Results ; Republic of Korea ; Reverse Transcriptase Polymerase Chain Reaction/standards ; Vero Cells
Czasopismo naukowe
Tytuł :
Direct RT-PCR amplification of SARS-CoV-2 from clinical samples using a concentrated viral lysis-amplification buffer prepared with IGEPAL-630.
Autorzy :
Castellanos-Gonzalez A; Infectious Diseases Division, Internal Medicine Department, University of Texas Medical Branch, Galveston, TX, USA. .
Shelite TR; Infectious Diseases Division, Internal Medicine Department, University of Texas Medical Branch, Galveston, TX, USA.
Lloyd N; Infectious Diseases Division, Pathology Department, University of Texas Medical Branch, Galveston, TX, USA.
Sadiqova A; Infectious Diseases Division, Internal Medicine Department, University of Texas Medical Branch, Galveston, TX, USA.
Ping R; Infectious Diseases Division, Pathology Department, University of Texas Medical Branch, Galveston, TX, USA.
Williams-Bouyer N; Infectious Diseases Division, Pathology Department, University of Texas Medical Branch, Galveston, TX, USA.
Melby PC; Infectious Diseases Division, Internal Medicine Department, University of Texas Medical Branch, Galveston, TX, USA.
Travi BL; Infectious Diseases Division, Internal Medicine Department, University of Texas Medical Branch, Galveston, TX, USA.
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Źródło :
Scientific reports [Sci Rep] 2021 Jul 09; Vol. 11 (1), pp. 14204. Date of Electronic Publication: 2021 Jul 09.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
MeSH Terms :
COVID-19*/diagnosis
COVID-19*/genetics
COVID-19 Nucleic Acid Testing*
Reverse Transcriptase Polymerase Chain Reaction*
Specimen Handling*
RNA, Viral/*genetics
SARS-CoV-2/*genetics
Humans ; Sensitivity and Specificity
Czasopismo naukowe
Tytuł :
Characteristics of SARS-CoV-2 testing for rapid diagnosis of COVID-19 during the initial stages of a global pandemic.
Autorzy :
Guthrie JL; Public Health Ontario, Toronto, Ontario, Canada.
Chen AJ; Public Health Ontario, Toronto, Ontario, Canada.
Budhram DR; Public Health Ontario, Toronto, Ontario, Canada.
Cronin K; Public Health Ontario, Toronto, Ontario, Canada.; National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.
Peci A; Public Health Ontario, Toronto, Ontario, Canada.
Nelson P; Public Health Ontario, Toronto, Ontario, Canada.
Mallo GV; Public Health Ontario, Toronto, Ontario, Canada.
Broukhanski G; Public Health Ontario, Toronto, Ontario, Canada.
Murti M; Public Health Ontario, Toronto, Ontario, Canada.; Dalla Lana School of Public Health, University of Toronto, Toronto, Ontario, Canada.
Majury A; Public Health Ontario, Toronto, Ontario, Canada.; Public Health Ontario, Kingston, Ontario, Canada.; Queen's University, Kingston, Ontario, Canada.
Mazzulli T; Public Health Ontario, Toronto, Ontario, Canada.; Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada.; Mount Sinai Hospital, Toronto, Canada.
Allen VG; Public Health Ontario, Toronto, Ontario, Canada.; Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada.
Patel SN; Public Health Ontario, Toronto, Ontario, Canada.; Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada.
Kus JV; Public Health Ontario, Toronto, Ontario, Canada.; Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada.
Tran V; Public Health Ontario, Toronto, Ontario, Canada.; Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada.
Gubbay JB; Public Health Ontario, Toronto, Ontario, Canada.; Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada.
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Źródło :
PloS one [PLoS One] 2021 Jul 09; Vol. 16 (7), pp. e0253941. Date of Electronic Publication: 2021 Jul 09 (Print Publication: 2021).
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
COVID-19*/diagnosis
COVID-19*/epidemiology
COVID-19*/genetics
COVID-19 Nucleic Acid Testing*
Pandemics*
Reverse Transcriptase Polymerase Chain Reaction*
SARS-CoV-2/*genetics
Adolescent ; Adult ; Aged ; Child ; Child, Preschool ; Female ; Humans ; Infant ; Male ; Middle Aged ; Ontario/epidemiology
Czasopismo naukowe
Tytuł :
Oropharyngeal Squamous Cell Carcinoma With Discordant p16 and HPV mRNA Results: Incidence and Characterization in a Large, Contemporary United States Cohort.
Autorzy :
Shinn JR; Department of Otorhinolaryngology-Head and Neck Surgery, University of Pennsylvania, Philadelphia, PA.
Davis SJ; Departments of Otolaryngology-Head and Neck Surgery.
Lang-Kuhs KA; Departments of Otolaryngology-Head and Neck Surgery.; Department of Epidemiology, College of Public Health, University of Kentucky, Lexington, KY.
Rohde S; Departments of Otolaryngology-Head and Neck Surgery.
Wang X; Department of Pharmacology and Regenerative Medicine, The University of Illinois at Chicago, Chicago, IL.
Liu P; Departments of Pathology and Immunology.
Dupont WD; Biostatistics.
Plummer D Jr; Biostatistics.
Thorstad WL; Radiation Oncology, Washington University School of Medicine, St. Louis, MO.
Chernock RD; Departments of Pathology and Immunology.
Mehrad M; Departments of Otolaryngology-Head and Neck Surgery.; Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN.
Lewis JS Jr; Departments of Otolaryngology-Head and Neck Surgery.; Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN.
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Źródło :
The American journal of surgical pathology [Am J Surg Pathol] 2021 Jul 01; Vol. 45 (7), pp. 951-961.
Typ publikacji :
Journal Article; Multicenter Study; Research Support, N.I.H., Extramural
MeSH Terms :
Immunohistochemistry*
Reverse Transcriptase Polymerase Chain Reaction*
Biomarkers, Tumor/*analysis
Cyclin-Dependent Kinase Inhibitor p16/*analysis
Oropharyngeal Neoplasms/*chemistry
Papillomaviridae/*genetics
Papillomavirus Infections/*diagnosis
RNA, Messenger/*genetics
RNA, Viral/*genetics
Squamous Cell Carcinoma of Head and Neck/*chemistry
Adult ; Aged ; Algorithms ; Decision Support Techniques ; Female ; Humans ; Incidence ; Male ; Middle Aged ; Oropharyngeal Neoplasms/mortality ; Oropharyngeal Neoplasms/therapy ; Oropharyngeal Neoplasms/virology ; Papillomavirus Infections/mortality ; Papillomavirus Infections/therapy ; Papillomavirus Infections/virology ; Predictive Value of Tests ; Prognosis ; Retrospective Studies ; Squamous Cell Carcinoma of Head and Neck/mortality ; Squamous Cell Carcinoma of Head and Neck/therapy ; Squamous Cell Carcinoma of Head and Neck/virology ; United States/epidemiology
Czasopismo naukowe
Tytuł :
Does the absence of SARS-CoV-2 specific genes always exclude the infection? How to interpret RT-PCR results?-The scenario of interactive online workshop.
Autorzy :
Kocemba-Pilarczyk KA; Jagiellonian University Medical College, Kraków, Poland.
Bentke-Imiolek A; Jagiellonian University Medical College, Kraków, Poland.
Dudzik P; Jagiellonian University Medical College, Kraków, Poland.
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Źródło :
Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology [Biochem Mol Biol Educ] 2021 Jul; Vol. 49 (4), pp. 658-663. Date of Electronic Publication: 2021 Mar 27.
Typ publikacji :
Journal Article
MeSH Terms :
COVID-19*/diagnosis
COVID-19*/genetics
COVID-19 Nucleic Acid Testing*
Education*
Education, Distance*
Reverse Transcriptase Polymerase Chain Reaction*
Molecular Biology/*education
SARS-CoV-2/*genetics
Humans
Czasopismo naukowe
Tytuł :
A rapid sap-direct reverse transcription-polymerase chain reaction method for detection of dendrobium viroid in Dendrobium plants.
Autorzy :
Li S; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.; Citrus Research Institute, Chinese Academy of Agricultural Sciences/Southwest University, Chongqing, China.
Yang Y; Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, China.
Xing F; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
Che HY; Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, China.
Cao XR; Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, China.
Zhang ZX; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
Khoo YW; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
Zhou CY; Citrus Research Institute, Chinese Academy of Agricultural Sciences/Southwest University, Chongqing, China.
Li SF; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.; Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, China.
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Źródło :
Letters in applied microbiology [Lett Appl Microbiol] 2021 Jul; Vol. 73 (1), pp. 26-30. Date of Electronic Publication: 2021 May 19.
Typ publikacji :
Journal Article
MeSH Terms :
Reverse Transcriptase Polymerase Chain Reaction*
Dendrobium/*virology
Viroids/*genetics
Virology/*methods
China ; Reverse Transcription ; Viroids/isolation & purification
Czasopismo naukowe
Tytuł :
Comparative evaluation of a dual-target real-time RT-PCR assay for COVID-19 diagnosis and assessment of performance in pooled saliva and nasopharyngeal swab samples.
Autorzy :
Yip CCY; Department of Microbiology, Queen Mary Hospital, Hong Kong Special Administrative Region, China.
Leung KH; Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong Special Administrative Region, China.
Ng ACK; Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong Special Administrative Region, China.
Chan KH; Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong Special Administrative Region, China.
To KKW; Department of Clinical Microbiology and Infection Control, The University of Hong Kong-Shenzhen Hospital, Shenzhen, Guangdong, China.; State Key Laboratory of Emerging Infectious Diseases, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong Special Administrative Region, China.; Carol Yu Centre for Infection, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong Special Administrative Region, China.
Chan JFW; Department of Clinical Microbiology and Infection Control, The University of Hong Kong-Shenzhen Hospital, Shenzhen, Guangdong, China.; State Key Laboratory of Emerging Infectious Diseases, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong Special Administrative Region, China.; Carol Yu Centre for Infection, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong Special Administrative Region, China.
Hung IFN; Department of Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong Special Administrative Region, China.
Cheng VCC; Department of Microbiology, Queen Mary Hospital, Hong Kong Special Administrative Region, China.
Sridhar S; Department of Clinical Microbiology and Infection Control, The University of Hong Kong-Shenzhen Hospital, Shenzhen, Guangdong, China.; State Key Laboratory of Emerging Infectious Diseases, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong Special Administrative Region, China.; Carol Yu Centre for Infection, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong Special Administrative Region, China.
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Źródło :
Expert review of molecular diagnostics [Expert Rev Mol Diagn] 2021 Jul; Vol. 21 (7), pp. 741-747. Date of Electronic Publication: 2021 Jun 04.
Typ publikacji :
Journal Article; Video-Audio Media
MeSH Terms :
COVID-19*/diagnosis
COVID-19*/genetics
COVID-19 Nucleic Acid Testing*
Real-Time Polymerase Chain Reaction*
Reverse Transcriptase Polymerase Chain Reaction*
Specimen Handling*
SARS-CoV-2/*genetics
Humans ; RNA, Viral/genetics
Czasopismo naukowe
Tytuł :
The use of routine blood tests to assist the diagnosis of COVID-19 in symptomatic hospitalized patients.
Autorzy :
Parsons IT; School of Life Sciences and Medicine, King's College London, St Thomas' Hospital, London, UK.
Parsons AT; School of Electronic, Electrical & Systems Engineering, Edgbaston, University of Birmingham, Birmingham, UK.
Balme E; Royal Surrey County Hospital NHS Trust, Guildford, UK.
Hazell G; Royal Surrey County Hospital NHS Trust, Guildford, UK.
Gifford R; Academic Department of Military Medicine, Research and Clinical Innovation, Royal Centre for Defence Medicine, Birmingham, UK.
Stacey M; Academic Department of Military Medicine, Research and Clinical Innovation, Royal Centre for Defence Medicine, Birmingham, UK.
Woods DR; Academic Department of Military Medicine, Research and Clinical Innovation, Royal Centre for Defence Medicine, Birmingham, UK.
Russell-Jones D; Royal Surrey County Hospital NHS Trust, Guildford, UK.
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Źródło :
Annals of clinical biochemistry [Ann Clin Biochem] 2021 Jul; Vol. 58 (4), pp. 318-326. Date of Electronic Publication: 2021 Mar 09.
Typ publikacji :
Journal Article
MeSH Terms :
COVID-19*/blood
COVID-19*/diagnosis
COVID-19 Nucleic Acid Testing*
Reverse Transcriptase Polymerase Chain Reaction*
SARS-CoV-2*
Ferritins/*blood
L-Lactate Dehydrogenase/*blood
Aged ; Aged, 80 and over ; Female ; Humans ; Leukocyte Count ; Male ; Middle Aged ; Retrospective Studies
Czasopismo naukowe
Tytuł :
SARS-CoV-2 RT-PCR test results across symptomatic COVID-19 cases in Auckland, New Zealand, February-June 2020.
Autorzy :
Howe K
Hale M
Reynolds GE
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Źródło :
Communicable diseases intelligence (2018) [Commun Dis Intell (2018)] 2021 Jun 15; Vol. 45. Date of Electronic Publication: 2021 Jun 15.
Typ publikacji :
Journal Article
MeSH Terms :
Reverse Transcriptase Polymerase Chain Reaction*
SARS-CoV-2*
COVID-19/*diagnosis
COVID-19/*epidemiology
Adolescent ; Adult ; Aged ; Aged, 80 and over ; COVID-19/mortality ; Child ; Child, Preschool ; Female ; Humans ; Infant ; Male ; Middle Aged ; New Zealand/epidemiology ; Young Adult
Czasopismo naukowe
Tytuł :
Development of an efficient one-step real-time reverse transcription polymerase chain reaction method for severe acute respiratory syndrome-coronavirus-2 detection.
Autorzy :
Nakura Y; Department of Developmental Medicine, Research Institute, Osaka Women's and Children's Hospital, Izumi-city, Osaka, Japan.
Wu HN; Department of Developmental Medicine, Research Institute, Osaka Women's and Children's Hospital, Izumi-city, Osaka, Japan.
Okamoto Y; Department of Laboratory Medicine, Osaka Women's and Children's Hospital, Izumi-city, Osaka, Japan.
Takeuchi M; Department of Intensive Care Medicine, Osaka Women's and Children's Hospital, Izumi-city, Osaka, Japan.
Suzuki K; The Research Foundation for Microbial Diseases of Osaka University, Suita-city, Osaka, Japan.
Tamura Y; Department of Clinical Laboratory, Osaka Habikino Medical Center, Habikino-city, Osaka, Japan.
Oba Y; Department of General Medicine, Osaka General Medical Center, Osaka-city, Osaka, Japan.
Nishiumi F; Department of Developmental Medicine, Research Institute, Osaka Women's and Children's Hospital, Izumi-city, Osaka, Japan.
Hatori N; The Research Foundation for Microbial Diseases of Osaka University, Suita-city, Osaka, Japan.
Fujiwara S; Department of Biosciences, School of Biological and Environmental Sciences, Kwansei-Gakuin University, Sanda-city, Hyogo, Japan.
Yasukawa K; Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Kyoto-city, Kyoto, Japan.
Ida S; Department of Laboratory Medicine, Osaka Women's and Children's Hospital, Izumi-city, Osaka, Japan.
Yanagihara I; Department of Developmental Medicine, Research Institute, Osaka Women's and Children's Hospital, Izumi-city, Osaka, Japan.; Department of Laboratory Medicine, Osaka Women's and Children's Hospital, Izumi-city, Osaka, Japan.
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Źródło :
PloS one [PLoS One] 2021 Jun 04; Vol. 16 (6), pp. e0252789. Date of Electronic Publication: 2021 Jun 04 (Print Publication: 2021).
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
COVID-19*/diagnosis
COVID-19*/genetics
Real-Time Polymerase Chain Reaction*
Reverse Transcriptase Polymerase Chain Reaction*
SARS-CoV-2/*genetics
Humans
Czasopismo naukowe
Tytuł :
Coronavirus Disease 2019 Test Correlation Between Nasopharyngeal Swab and BAL in Asymptomatic Patients.
Autorzy :
Chang J; Division of Pulmonary, Allergy and Critical Care Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA.
Swenson KE; Division of Pulmonary, Allergy and Critical Care Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA.
Sung A; Division of Pulmonary, Allergy and Critical Care Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA.
Bedi H; Division of Pulmonary, Allergy and Critical Care Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA. Electronic address: .
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Źródło :
Chest [Chest] 2021 Jun; Vol. 159 (6), pp. 2488-2490. Date of Electronic Publication: 2020 Nov 17.
Typ publikacji :
Letter
MeSH Terms :
COVID-19 Nucleic Acid Testing*
Reverse Transcriptase Polymerase Chain Reaction*
Bronchoalveolar Lavage Fluid/*virology
COVID-19/*diagnosis
Carrier State/*diagnosis
Nasopharynx/*virology
Adult ; Aged ; Bronchoalveolar Lavage ; Bronchoscopy ; False Negative Reactions ; False Positive Reactions ; Female ; Humans ; Male ; Middle Aged ; Prospective Studies ; Reproducibility of Results
Opinia redakcyjna
Tytuł :
Detection of PIK3CA Gene Mutation in Head and Neck Squamous Cell Carcinoma Using Droplet Digital PCR and RT-qPCR.
Autorzy :
Borkowska EM; Department of Clinical Genetics Chair of Laboratory and Clinical Genetics, Medical University of Lodz, 92-213 Lodz, Poland.
Barańska M; Department of Otolaryngology, Head and Neck Oncology, Medical University of Lodz, 93-143 Lodz, Poland.
Kowalczyk M; Department of Otolaryngology, Head and Neck Oncology, Medical University of Lodz, 93-143 Lodz, Poland.
Pietruszewska W; Department of Otolaryngology, Head and Neck Oncology, Medical University of Lodz, 93-143 Lodz, Poland.
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Źródło :
Biomolecules [Biomolecules] 2021 May 31; Vol. 11 (6). Date of Electronic Publication: 2021 May 31.
Typ publikacji :
Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Real-Time Polymerase Chain Reaction*
Reverse Transcriptase Polymerase Chain Reaction*
Class I Phosphatidylinositol 3-Kinases/*genetics
Head and Neck Neoplasms/*genetics
Neoplasm Proteins/*genetics
Squamous Cell Carcinoma of Head and Neck/*genetics
Adult ; Aged ; Aged, 80 and over ; Female ; Head and Neck Neoplasms/enzymology ; Humans ; Male ; Middle Aged ; Squamous Cell Carcinoma of Head and Neck/enzymology
Czasopismo naukowe
Tytuł :
Correlating qRT-PCR, dPCR and Viral Titration for the Identification and Quantification of SARS-CoV-2: A New Approach for Infection Management.
Autorzy :
Brandolini M; Unit of Microbiology, The Great Romagna Hub Laboratory, 47522 Pievesestina, Italy.
Taddei F; Unit of Microbiology, The Great Romagna Hub Laboratory, 47522 Pievesestina, Italy.
Marino MM; Unit of Microbiology, The Great Romagna Hub Laboratory, 47522 Pievesestina, Italy.
Grumiro L; Unit of Microbiology, The Great Romagna Hub Laboratory, 47522 Pievesestina, Italy.
Scalcione A; Unit of Microbiology, The Great Romagna Hub Laboratory, 47522 Pievesestina, Italy.
Turba ME; Xenturion Srl, 47122 Forlì, Italy.
Gentilini F; Department of Veterinary Medical Sciences, Alma Mater Studiorum-University of Bologna, 40064 Bologna, Italy.
Fantini M; Unit of Microbiology, The Great Romagna Hub Laboratory, 47522 Pievesestina, Italy.
Zannoli S; Unit of Microbiology, The Great Romagna Hub Laboratory, 47522 Pievesestina, Italy.
Dirani G; Unit of Microbiology, The Great Romagna Hub Laboratory, 47522 Pievesestina, Italy.
Sambri V; Unit of Microbiology, The Great Romagna Hub Laboratory, 47522 Pievesestina, Italy.; Department of Experimental, Diagnostic and Specialty Medicine-DIMES, Alma Mater Studiorum-University of Bologna, 40138 Bologna, Italy.
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Źródło :
Viruses [Viruses] 2021 May 28; Vol. 13 (6). Date of Electronic Publication: 2021 May 28.
Typ publikacji :
Comparative Study; Journal Article
MeSH Terms :
Reverse Transcriptase Polymerase Chain Reaction*
COVID-19/*therapy
COVID-19/*virology
Polymerase Chain Reaction/*methods
RNA, Viral/*analysis
SARS-CoV-2/*isolation & purification
Viral Load/*methods
Animals ; Cells, Cultured ; Chlorocebus aethiops ; Digital Technology/methods ; Humans ; SARS-CoV-2/genetics ; Vero Cells ; Virus Cultivation
Czasopismo naukowe
Tytuł :
SARS-CoV-2 PCR-positive and PCR-negative cases of pneumonia admitted to the hospital during the peak of COVID-19 pandemic: analysis of in-hospital and post-hospital mortality.
Autorzy :
Gaipov A; Department of Medicine, Nazarbayev University School of Medicine, Kerey and Zhanibek Khans Street 5/1, Room 345, Nur-Sultan city, Kazakhstan. .
Gusmanov A; Department of Medicine, Nazarbayev University School of Medicine, Kerey and Zhanibek Khans Street 5/1, Room 345, Nur-Sultan city, Kazakhstan.
Abbay A; Department of Medicine, Nazarbayev University School of Medicine, Kerey and Zhanibek Khans Street 5/1, Room 345, Nur-Sultan city, Kazakhstan.
Sakko Y; Department of Medicine, Nazarbayev University School of Medicine, Kerey and Zhanibek Khans Street 5/1, Room 345, Nur-Sultan city, Kazakhstan.
Issanov A; Department of Medicine, Nazarbayev University School of Medicine, Kerey and Zhanibek Khans Street 5/1, Room 345, Nur-Sultan city, Kazakhstan.
Kadyrzhanuly K; Department of Medicine, Nazarbayev University School of Medicine, Kerey and Zhanibek Khans Street 5/1, Room 345, Nur-Sultan city, Kazakhstan.
Yermakhanova Z; Department of Emergency Medicine, Akhmet Yassawi University Medical Faculty, Turkestan, Kazakhstan.
Aliyeva L; Department of expertise, Social Health Insurance Fund branch of the Turkestan Region, Turkestan, Kazakhstan.
Kashkynbayev A; Department of Mathematics, Nazarbayev University School of Sciences and Humanities, Nur-Sultan, Kazakhstan.
Moldaliyev I; Department of Preventive Medicine, Akhmet Yassawi University Medical Faculty, Turkestan, Kazakhstan.
Crape B; Department of Medicine, Nazarbayev University School of Medicine, Kerey and Zhanibek Khans Street 5/1, Room 345, Nur-Sultan city, Kazakhstan.
Sarria-Santamera A; Department of Medicine, Nazarbayev University School of Medicine, Kerey and Zhanibek Khans Street 5/1, Room 345, Nur-Sultan city, Kazakhstan.
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Źródło :
BMC infectious diseases [BMC Infect Dis] 2021 May 20; Vol. 21 (1), pp. 458. Date of Electronic Publication: 2021 May 20.
Typ publikacji :
Journal Article
MeSH Terms :
Reverse Transcriptase Polymerase Chain Reaction*
COVID-19/*mortality
Pneumonia/*diagnosis
RNA, Viral/*metabolism
SARS-CoV-2/*genetics
Adult ; Aged ; COVID-19/diagnosis ; COVID-19/epidemiology ; COVID-19/virology ; Female ; Hospital Mortality ; Hospitalization ; Humans ; Kazakhstan/epidemiology ; Male ; Middle Aged ; Pandemics ; Pneumonia/mortality ; Pneumonia/virology ; RNA, Viral/analysis ; SARS-CoV-2/isolation & purification ; Survival Rate ; Young Adult
Czasopismo naukowe
Tytuł :
Environmentally friendly method of RNA isolation.
Autorzy :
Drygin YF; Laboratory of Molecular Biology of Viruses, Belozersky Research Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russian Federation; Laboratory of Immunochemistry, Research Center of Biotechnology, Russian Academy of Sciences, Moscow, Russian Federation. Electronic address: .
Butenko KO; Laboratory of Immunochemistry, Research Center of Biotechnology, Russian Academy of Sciences, Moscow, Russian Federation; Chair of Virology, Faculty of Biology, Lomonosov Moscow State University, Moscow, Russian Federation.
Gasanova TV; Chair of Virology, Faculty of Biology, Lomonosov Moscow State University, Moscow, Russian Federation.
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Źródło :
Analytical biochemistry [Anal Biochem] 2021 May 01; Vol. 620, pp. 114113. Date of Electronic Publication: 2021 Jan 30.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Reverse Transcriptase Polymerase Chain Reaction*
Escherichia coli/*genetics
RNA/*isolation & purification
Tobacco/*genetics
RNA/genetics
Czasopismo naukowe
Tytuł :
Development and application of a real-time RT-PCR assay to rapidly detect H2 subtype avian influenza A viruses.
Autorzy :
Xiao Y; State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, and National Clinical Research Center for Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
Yang F; State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, and National Clinical Research Center for Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
Liu F; State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, and National Clinical Research Center for Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
Yao H; State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, and National Clinical Research Center for Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
Wu N; State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, and National Clinical Research Center for Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
Wu H; State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, and National Clinical Research Center for Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
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Źródło :
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc [J Vet Diagn Invest] 2021 May; Vol. 33 (3), pp. 577-581. Date of Electronic Publication: 2021 Feb 22.
Typ publikacji :
Journal Article
MeSH Terms :
Influenza A virus/*isolation & purification
Influenza in Birds/*diagnosis
Real-Time Polymerase Chain Reaction/*veterinary
Reverse Transcriptase Polymerase Chain Reaction/*veterinary
Animals ; Chick Embryo ; Chickens ; Real-Time Polymerase Chain Reaction/methods ; Reverse Transcriptase Polymerase Chain Reaction/methods ; Sensitivity and Specificity
Czasopismo naukowe

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