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Wyświetlanie 1-18 z 18
Tytuł :
Enzymatic degradation of PrPSc by a protease secreted from Aeropyrum pernix K1.
Autorzy :
Snajder M; Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.
Vilfan T
Cernilec M
Rupreht R
Popović M
Juntes P
Serbec VČ
Ulrih NP
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Źródło :
PloS one [PLoS One] 2012; Vol. 7 (6), pp. e39548. Date of Electronic Publication: 2012 Jun 28.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Aeropyrum/*chemistry
Metalloproteases/*analysis
PrPSc Proteins/*metabolism
Aeropyrum/metabolism ; Animals ; Blotting, Western ; Brain/metabolism ; Cattle ; Electrophoresis, Polyacrylamide Gel ; Humans ; Metalloproteases/metabolism ; Mice ; Protein Isoforms/metabolism
Czasopismo naukowe
Tytuł :
Codon optimisation is key for pernisine expression in Escherichia coli.
Autorzy :
Šnajder M; Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.
Mihelič M; Centre of Excellence for Integrated Approaches in Chemistry and Biology (CipKeBiP), Ljubljana, Slovenia; Institute Jozef Stefan, Ljubljana, Slovenia.
Turk D; Centre of Excellence for Integrated Approaches in Chemistry and Biology (CipKeBiP), Ljubljana, Slovenia; Institute Jozef Stefan, Ljubljana, Slovenia.
Ulrih NP; Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia; Centre of Excellence for Integrated Approaches in Chemistry and Biology (CipKeBiP), Ljubljana, Slovenia.
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Źródło :
PloS one [PLoS One] 2015 Apr 09; Vol. 10 (4), pp. e0123288. Date of Electronic Publication: 2015 Apr 09 (Print Publication: 2015).
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Gene Expression Regulation, Bacterial*
Archaea/*genetics
Escherichia coli/*genetics
Serine Proteases/*biosynthesis
Amino Acid Sequence ; Archaea/enzymology ; Cloning, Molecular ; Codon/genetics ; Escherichia coli/enzymology ; Serine Proteases/genetics
Czasopismo naukowe
Tytuł :
Characterization of a novel high-pH-tolerant laccase-like multicopper oxidase and its sequence diversity in Thioalkalivibrio sp.
Autorzy :
Ausec L; Department of Food Science and Technology, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000, Ljubljana, Slovenia.
Črnigoj M; Department of Food Science and Technology, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000, Ljubljana, Slovenia.; Department of Biology, Biotechnical Faculty, University of Ljubljana, Večna pot 111, 1000, Ljubljana, Slovenia.
Šnajder M; Department of Food Science and Technology, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000, Ljubljana, Slovenia.
Ulrih NP; Department of Food Science and Technology, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000, Ljubljana, Slovenia.; Centre of Excellence for Integrated Approaches in Chemistry and Biology of Proteins (CipKeBiP), Jamova 39, 1000, Ljubljana, Slovenia.
Mandic-Mulec I; Department of Food Science and Technology, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000, Ljubljana, Slovenia. .
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Źródło :
Applied microbiology and biotechnology [Appl Microbiol Biotechnol] 2015 Dec; Vol. 99 (23), pp. 9987-99. Date of Electronic Publication: 2015 Jul 31.
Typ publikacji :
Journal Article
MeSH Terms :
Genetic Variation*
Ectothiorhodospiraceae/*enzymology
Laccase/*metabolism
Recombinant Fusion Proteins/*metabolism
Amino Acid Sequence ; Cloning, Molecular ; Cluster Analysis ; Ectothiorhodospiraceae/genetics ; Enzyme Stability ; Escherichia coli/genetics ; Escherichia coli/metabolism ; Gene Expression ; Hydrogen-Ion Concentration ; Laccase/chemistry ; Laccase/genetics ; Laccase/isolation & purification ; Molecular Sequence Data ; Phylogeny ; Recombinant Fusion Proteins/chemistry ; Recombinant Fusion Proteins/genetics ; Recombinant Fusion Proteins/isolation & purification ; Sequence Homology, Amino Acid ; Substrate Specificity ; Temperature
Czasopismo naukowe
Tytuł :
Extracellular production of the engineered thermostable protease pernisine from Aeropyrum pernix K1 in Streptomyces rimosus.
Autorzy :
Šnajder M; Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000, Ljubljana, Slovenia.; Labena Ltd., Ljubljana, Slovenia.
Carrillo Rincón AF; Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000, Ljubljana, Slovenia.
Magdevska V; Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000, Ljubljana, Slovenia.; AciesBio Ltd., Ljubljana, Slovenia.
Bahun M; Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000, Ljubljana, Slovenia.
Kranjc L; Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000, Ljubljana, Slovenia.
Paš M; Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000, Ljubljana, Slovenia.
Juntes P; Veterinary Faculty, University of Ljubljana, Ljubljana, Slovenia.
Petković H; Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000, Ljubljana, Slovenia. .
Poklar Ulrih N; Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000, Ljubljana, Slovenia. .; The Centre of Excellence for Integrated Approaches in Chemistry and Biology of Proteins, Ljubljana, Slovenia. .
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Źródło :
Microbial cell factories [Microb Cell Fact] 2019 Nov 07; Vol. 18 (1), pp. 196. Date of Electronic Publication: 2019 Nov 07.
Typ publikacji :
Journal Article
MeSH Terms :
Bacterial Proteins*/genetics
Bacterial Proteins*/metabolism
Endopeptidases*/genetics
Endopeptidases*/metabolism
Microorganisms, Genetically-Modified*/genetics
Microorganisms, Genetically-Modified*/metabolism
Recombinant Proteins*/genetics
Recombinant Proteins*/metabolism
Streptomyces rimosus*/genetics
Streptomyces rimosus*/metabolism
Aeropyrum/*enzymology
Cloning, Molecular
Czasopismo naukowe
Tytuł :
Computational design and characterization of nanobody-derived peptides that stabilize the active conformation of the β 2 -adrenergic receptor (β 2 -AR).
Autorzy :
Sencanski M; Center for Multidisciplinary Research, Institute of Nuclear Sciences VINCA, University of Belgrade, Belgrade, Serbia.
Glisic S; Center for Multidisciplinary Research, Institute of Nuclear Sciences VINCA, University of Belgrade, Belgrade, Serbia.
Šnajder M; Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.
Veljkovic N; Center for Multidisciplinary Research, Institute of Nuclear Sciences VINCA, University of Belgrade, Belgrade, Serbia.
Poklar Ulrih N; Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.
Mavri J; Laboratory of Computational Biochemistry and Drug Design, National Institute of Chemistry, Ljubljana, Slovenia.
Vrecl M; Institute of Preclinical Sciences, Veterinary Faculty, University of Ljubljana, Ljubljana, Slovenia. .
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Źródło :
Scientific reports [Sci Rep] 2019 Nov 12; Vol. 9 (1), pp. 16555. Date of Electronic Publication: 2019 Nov 12.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Peptides/*chemistry
Receptors, Adrenergic, beta-2/*metabolism
HEK293 Cells ; Humans ; Ligands ; Molecular Dynamics Simulation ; Protein Binding ; Protein Domains ; Protein Stability ; Single-Domain Antibodies
Czasopismo naukowe
Tytuł :
Insights into the Maturation of Pernisine, a Subtilisin-Like Protease from the Hyperthermophilic Archaeon Aeropyrum pernix.
Autorzy :
Bahun M; Department of Food Science and Technology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.
Šnajder M; Department of Food Science and Technology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.
Turk D; Department of Molecular and Structural Biology, Jožef Stefan Institute, Ljubljana, Slovenia.; Centre of Excellence for Integrated Approaches in Chemistry and Biology of Proteins (CIPKeBiP), Ljubljana, Slovenia.
Poklar Ulrih N; Department of Food Science and Technology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia .; Centre of Excellence for Integrated Approaches in Chemistry and Biology of Proteins (CIPKeBiP), Ljubljana, Slovenia.
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Źródło :
Applied and environmental microbiology [Appl Environ Microbiol] 2020 Aug 18; Vol. 86 (17). Date of Electronic Publication: 2020 Aug 18 (Print Publication: 2020).
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Aeropyrum/*genetics
Archaeal Proteins/*genetics
Peptide Hydrolases/*genetics
Aeropyrum/enzymology ; Amino Acid Sequence ; Archaeal Proteins/chemistry ; Archaeal Proteins/metabolism ; Binding Sites ; Molecular Conformation ; Peptide Hydrolases/chemistry ; Peptide Hydrolases/metabolism
Czasopismo naukowe
    Wyświetlanie 1-18 z 18

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