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Tytuł:
Rapid Detection of Three Common Bacteria Based on Fluorescence Spectroscopy.
Autorzy:
Du R; Guangdong Key Lab of Ocean Remote Sensing, State Key Laboratory of Tropical Oceanography, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China.; University of Chinese Academy of Sciences, Beijing 100049, China.
Yang D; Guangdong Key Lab of Ocean Remote Sensing, State Key Laboratory of Tropical Oceanography, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China.; Southern Marine Science and Engineering Guangdong Laboratory, Guangzhou 511458, China.
Yin X; Guangdong Key Lab of Ocean Remote Sensing, State Key Laboratory of Tropical Oceanography, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China.; University of Chinese Academy of Sciences, Beijing 100049, China.
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Źródło:
Sensors (Basel, Switzerland) [Sensors (Basel)] 2022 Feb 03; Vol. 22 (3). Date of Electronic Publication: 2022 Feb 03.
Typ publikacji:
Journal Article
MeSH Terms:
Escherichia coli*
Staphylococcus aureus*
Bacteria ; Fluorescence ; Spectrometry, Fluorescence ; Tryptophan
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknie Pełny tekstSzczegóły
Tytuł:
Biophysical and structural studies reveal marginal stability of a crucial hydrocarbon biosynthetic enzyme acyl ACP reductase.
Autorzy:
Sharma A; Microbial Engineering Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, India.; DBT-ICGEB Centre for Advanced Bioenergy Research, International Centre for Genetic Engineering and Biotechnology, New Delhi, India.
Shakeel T; Microbial Engineering Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, India.; DBT-ICGEB Centre for Advanced Bioenergy Research, International Centre for Genetic Engineering and Biotechnology, New Delhi, India.
Gupta M; Microbial Engineering Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, India.; DBT-ICGEB Centre for Advanced Bioenergy Research, International Centre for Genetic Engineering and Biotechnology, New Delhi, India.
Rajacharya GH; Microbial Engineering Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, India.; DBT-ICGEB Centre for Advanced Bioenergy Research, International Centre for Genetic Engineering and Biotechnology, New Delhi, India.
Yazdani SS; Microbial Engineering Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, India. .; DBT-ICGEB Centre for Advanced Bioenergy Research, International Centre for Genetic Engineering and Biotechnology, New Delhi, India. .
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Źródło:
Scientific reports [Sci Rep] 2021 Jun 08; Vol. 11 (1), pp. 12045. Date of Electronic Publication: 2021 Jun 08.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
Enoyl-(Acyl-Carrier Protein) Reductase (NADPH, B-Specific)/*metabolism
Escherichia coli/*enzymology
Hydrocarbons/*chemistry
Alkanes/metabolism ; Bacterial Proteins/metabolism ; Biofuels ; Biophysics ; Biotechnology ; Chromatography ; Chromatography, Liquid ; Circular Dichroism ; Light ; Mass Spectrometry ; Molecular Dynamics Simulation ; Oxygenases/chemistry ; Protein Denaturation ; Protein Folding ; Scattering, Radiation ; Spectrometry, Fluorescence ; Static Electricity ; Synechococcus/metabolism ; Temperature ; Urea/chemistry
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknieSzczegóły
Tytuł:
Affinity of Skp to OmpC revealed by single-molecule detection.
Autorzy:
Pan S; Beijing National Laboratory for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, and Department of Chemical Biology, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China.; Biomedical Pioneering Innovation Center (BIOPIC), Peking University, Beijing, 100871, China.
Yang C; Beijing National Laboratory for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, and Department of Chemical Biology, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China.; Biomedical Pioneering Innovation Center (BIOPIC), Peking University, Beijing, 100871, China.
Zhao XS; Beijing National Laboratory for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, and Department of Chemical Biology, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China. .; Biomedical Pioneering Innovation Center (BIOPIC), Peking University, Beijing, 100871, China. .
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Źródło:
Scientific reports [Sci Rep] 2020 Sep 10; Vol. 10 (1), pp. 14871. Date of Electronic Publication: 2020 Sep 10.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
DNA-Binding Proteins/*metabolism
Escherichia coli/*metabolism
Escherichia coli Proteins/*metabolism
Molecular Chaperones/*metabolism
Periplasm/*metabolism
Porins/*metabolism
DNA-Binding Proteins/chemistry ; Escherichia coli/growth & development ; Escherichia coli Proteins/chemistry ; Molecular Chaperones/chemistry ; Porins/chemistry ; Protein Binding ; Protein Folding ; Single Molecule Imaging/methods ; Spectrometry, Fluorescence/methods
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknie Pełny tekstSzczegóły
Tytuł:
Site-Specific Tryptophan Labels Reveal Local Microsecond-Millisecond Motions of Dihydrofolate Reductase.
Autorzy:
Vaughn MB; Division of Natural Sciences, Oglethorpe University, Atlanta, GA 30319, USA.
Biren C; Department of Chemistry, Emory University, Atlanta, GA 30322, USA.
Li Q; Department of Chemistry, Emory University, Atlanta, GA 30322, USA.
Ragupathi A; Department of Chemistry, Emory University, Atlanta, GA 30322, USA.
Dyer RB; Department of Chemistry, Emory University, Atlanta, GA 30322, USA.
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Źródło:
Molecules (Basel, Switzerland) [Molecules] 2020 Aug 22; Vol. 25 (17). Date of Electronic Publication: 2020 Aug 22.
Typ publikacji:
Journal Article
MeSH Terms:
Escherichia coli/*enzymology
Escherichia coli Proteins/*chemistry
Tetrahydrofolate Dehydrogenase/*chemistry
Escherichia coli/genetics ; Escherichia coli Proteins/genetics ; Mutation ; Protein Conformation ; Spectrometry, Fluorescence ; Tetrahydrofolate Dehydrogenase/genetics ; Tryptophan/chemistry ; Tryptophan/genetics
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknieSzczegóły
Tytuł:
Identification of resistance in Escherichia coli and Klebsiella pneumoniae using excitation-emission matrix fluorescence spectroscopy and multivariate analysis.
Autorzy:
Costa FSL; Institute of Chemistry, Biological Chemistry and Chemometrics, Federal University of Rio Grande do Norte, Natal, RN, 59072-970, Brazil.
Bezerra CCR; Laboratory of Mycobateria, Department of Microbiology and Parasitology, Federal University of Rio Grande do Norte, Natal, RN, 59072-970, Brazil.
Neto RM; Laboratory of Mycobateria, Department of Microbiology and Parasitology, Federal University of Rio Grande do Norte, Natal, RN, 59072-970, Brazil.
Morais CLM; Lancashire Teaching Hospitals NHS Trust, Fulwood, Preston, PR2 9HT, UK.
Lima KMG; Institute of Chemistry, Biological Chemistry and Chemometrics, Federal University of Rio Grande do Norte, Natal, RN, 59072-970, Brazil. .
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Źródło:
Scientific reports [Sci Rep] 2020 Aug 03; Vol. 10 (1), pp. 12994. Date of Electronic Publication: 2020 Aug 03.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
Drug Resistance, Bacterial*
Escherichia coli/*drug effects
Klebsiella pneumoniae/*drug effects
Spectrometry, Fluorescence/*methods
Microbial Sensitivity Tests ; Multivariate Analysis ; Principal Component Analysis ; Reproducibility of Results
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknie Pełny tekstSzczegóły
Tytuł:
Multimode detection of β-glycosidase and pathogenic bacteria via cation exchange assisted signal amplification.
Autorzy:
Wang X; College of Chemistry and Material Science, Sichuan Normal University, Chengdu, 610068, Sichuan, China.
Chen W; College of Chemistry and Chemical Engineering, China West Normal University, Nanchong, 637000, China.
Yang H; College of Chemistry and Material Science, Sichuan Normal University, Chengdu, 610068, Sichuan, China.
Zhang X; Interdisciplinary Nanoscience Center, Aarhus University, 8000, Aarhus C, Denmark.
Deng M; College of Chemistry and Material Science, Sichuan Normal University, Chengdu, 610068, Sichuan, China.
Zhou X; College of Chemistry and Material Science, Sichuan Normal University, Chengdu, 610068, Sichuan, China.
Huang K; College of Chemistry and Material Science, Sichuan Normal University, Chengdu, 610068, Sichuan, China. .
Chen P; Department of Laboratory Medicine, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University and Collaborative Innovation Center for Biotherapy, Chengdu, 610041, Sichuan, China. .
Ying B; Department of Laboratory Medicine, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University and Collaborative Innovation Center for Biotherapy, Chengdu, 610041, Sichuan, China. .
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Źródło:
Mikrochimica acta [Mikrochim Acta] 2020 Jul 17; Vol. 187 (8), pp. 453. Date of Electronic Publication: 2020 Jul 17.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
Bacterial Proteins/*analysis
Bacteriological Techniques/*methods
Enzyme Assays/*methods
Escherichia coli/*isolation & purification
beta-Galactosidase/*analysis
Animals ; Bacterial Proteins/chemistry ; Bacterial Proteins/urine ; Cadmium Compounds/chemistry ; Escherichia coli/enzymology ; Galactosides/chemistry ; Humans ; Limit of Detection ; Mass Spectrometry ; Metal Nanoparticles/chemistry ; Milk/microbiology ; Oxidation-Reduction ; Quantum Dots/chemistry ; Silver/chemistry ; Spectrometry, Fluorescence ; Tellurium/chemistry ; Urine/microbiology ; beta-Galactosidase/chemistry ; beta-Galactosidase/urine
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknie Pełny tekstSzczegóły
Tytuł:
Cationic liposomes for generic signal amplification strategies in bioassays.
Autorzy:
Hofmann C; Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, Universitätsstraße 31, 93053, Regensburg, Germany.
Kaiser B; Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, Universitätsstraße 31, 93053, Regensburg, Germany.
Maerkl S; Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, Universitätsstraße 31, 93053, Regensburg, Germany.
Duerkop A; Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, Universitätsstraße 31, 93053, Regensburg, Germany.
Baeumner AJ; Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, Universitätsstraße 31, 93053, Regensburg, Germany. .
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Źródło:
Analytical and bioanalytical chemistry [Anal Bioanal Chem] 2020 May; Vol. 412 (14), pp. 3383-3393. Date of Electronic Publication: 2020 Apr 06.
Typ publikacji:
Journal Article
MeSH Terms:
Cations/*chemistry
Escherichia coli/*isolation & purification
Fluorescent Dyes/*analysis
Liposomes/*chemistry
Rhodamines/*analysis
Bacterial Load/methods ; Escherichia coli Infections/microbiology ; Humans ; Luminescent Measurements/methods ; Phospholipids/chemistry ; Spectrometry, Fluorescence/methods ; Static Electricity
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknie Pełny tekstSzczegóły
Tytuł:
The challenge of intracellular antibiotic accumulation, a function of fluoroquinolone influx versus bacterial efflux.
Autorzy:
Vergalli J; UMR_MD1, U-1261, Aix-Marseille University, INSERM, IRBA, MCT, Marseille, France.
Atzori A; Department of Physics, University of Cagliari, 09042, Monserrato, CA, Italy.
Pajovic J; DISCO beamline, Synchrotron Soleil, Saint-Aubin, France.; University of Belgrade, Faculty of Physics, 11001, Belgrade, Serbia.
Dumont E; UMR_MD1, U-1261, Aix-Marseille University, INSERM, IRBA, MCT, Marseille, France.; Institut National Supérieur d'Agronomie et de Biotechnologies (INSAB), Université des Sciences et Techniques de Masuku (USTM), BP941, Franceville, Gabon.
Malloci G; Department of Physics, University of Cagliari, 09042, Monserrato, CA, Italy.
Masi M; UMR_MD1, U-1261, Aix-Marseille University, INSERM, IRBA, MCT, Marseille, France.; Institut de Biologie Intégrative de la Cellule (I2BC), Université Paris Saclay, CNRS, CEA, 91198, Gif-sur-Yvette, France.
Vargiu AV; Department of Physics, University of Cagliari, 09042, Monserrato, CA, Italy.
Winterhalter M; Department of Life Sciences and Chemistry, Jacobs University Bremen, 28719, Bremen, Germany.
Réfrégiers M; DISCO beamline, Synchrotron Soleil, Saint-Aubin, France.
Ruggerone P; Department of Physics, University of Cagliari, 09042, Monserrato, CA, Italy.
Pagès JM; UMR_MD1, U-1261, Aix-Marseille University, INSERM, IRBA, MCT, Marseille, France. .
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Źródło:
Communications biology [Commun Biol] 2020 Apr 28; Vol. 3 (1), pp. 198. Date of Electronic Publication: 2020 Apr 28.
Typ publikacji:
Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
MeSH Terms:
Anti-Bacterial Agents/*metabolism
Escherichia coli/*metabolism
Escherichia coli Proteins/*metabolism
Fluoroquinolones/*metabolism
Multidrug Resistance-Associated Proteins/*metabolism
Anti-Bacterial Agents/pharmacology ; Binding Sites ; Biological Transport ; Drug Resistance, Bacterial ; Escherichia coli/drug effects ; Escherichia coli/genetics ; Escherichia coli Proteins/chemistry ; Escherichia coli Proteins/genetics ; Fluoroquinolones/pharmacology ; Molecular Docking Simulation ; Molecular Dynamics Simulation ; Molecular Structure ; Multidrug Resistance-Associated Proteins/chemistry ; Multidrug Resistance-Associated Proteins/genetics ; Protein Binding ; Spectrometry, Fluorescence ; Structure-Activity Relationship
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknie Pełny tekstSzczegóły
Tytuł:
Rapid and cost-effective evaluation of bacterial viability using fluorescence spectroscopy.
Autorzy:
Ou F; Department of Physics, The University of Auckland, Auckland, 1010, New Zealand. .; The Dodd-Walls Centre for Photonic and Quantum Technologies, Auckland, 1010, New Zealand. .
McGoverin C; Department of Physics, The University of Auckland, Auckland, 1010, New Zealand.; The Dodd-Walls Centre for Photonic and Quantum Technologies, Auckland, 1010, New Zealand.
Swift S; School of Medical Sciences, The University of Auckland, Auckland, 1023, New Zealand.
Vanholsbeeck F; Department of Physics, The University of Auckland, Auckland, 1010, New Zealand.; The Dodd-Walls Centre for Photonic and Quantum Technologies, Auckland, 1010, New Zealand.
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Źródło:
Analytical and bioanalytical chemistry [Anal Bioanal Chem] 2019 Jun; Vol. 411 (16), pp. 3653-3663. Date of Electronic Publication: 2019 May 02.
Typ publikacji:
Journal Article
MeSH Terms:
Cost-Benefit Analysis*
Microbial Viability*
Escherichia coli/*physiology
Spectrometry, Fluorescence/*methods
Escherichia coli/isolation & purification ; Flow Cytometry ; Fluorescent Dyes/chemistry ; Organic Chemicals/chemistry ; Reproducibility of Results
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknie Pełny tekstSzczegóły
Tytuł:
Integration of a Gold-Specific Whole E. coli Cell Sensing and Adsorption Based on BioBrick.
Autorzy:
Yan L; State Key Laboratory of Coordination Chemistry, Institute of Chemistry and BioMedical Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China. .
Sun P; State Key Laboratory of Coordination Chemistry, Institute of Chemistry and BioMedical Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China. .; State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, China. .
Xu Y; State Key Laboratory of Coordination Chemistry, Institute of Chemistry and BioMedical Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China. .
Zhang S; College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China. .
Wei W; State Key Laboratory of Coordination Chemistry, Institute of Chemistry and BioMedical Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China. .
Zhao J; State Key Laboratory of Coordination Chemistry, Institute of Chemistry and BioMedical Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China. .; State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, China. .
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Źródło:
International journal of molecular sciences [Int J Mol Sci] 2018 Nov 24; Vol. 19 (12). Date of Electronic Publication: 2018 Nov 24.
Typ publikacji:
Journal Article
MeSH Terms:
Biosensing Techniques*
Environmental Pollution/*analysis
Escherichia coli/*genetics
Escherichia coli Proteins/*genetics
Gold/*chemistry
Metals, Heavy/*analysis
Water Pollutants, Chemical/*analysis
Adsorption ; Carrier Proteins ; Escherichia coli/metabolism ; Escherichia coli Proteins/metabolism ; Genes, Reporter ; Genetic Engineering/methods ; Humans ; Limit of Detection ; Luminescent Proteins/genetics ; Luminescent Proteins/metabolism ; Promoter Regions, Genetic ; Recombinant Proteins/genetics ; Recombinant Proteins/metabolism ; Regulon ; Salmonella typhimurium/genetics ; Salmonella typhimurium/metabolism ; Spectrometry, Fluorescence/methods ; Red Fluorescent Protein
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknie Pełny tekstSzczegóły
Tytuł:
Biosynthesis of Fluorescent β Subunits of C-Phycocyanin from Spirulina subsalsa in Escherichia coli , and Their Antioxidant Properties.
Autorzy:
Wu XJ; College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, China. .
Yang H; College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, China. .
Chen YT; College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, China. .
Li PP; College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, China. .
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Źródło:
Molecules (Basel, Switzerland) [Molecules] 2018 Jun 06; Vol. 23 (6). Date of Electronic Publication: 2018 Jun 06.
Typ publikacji:
Journal Article
MeSH Terms:
Antioxidants/*pharmacology
Escherichia coli/*genetics
Fluorescent Dyes/*chemistry
Phycocyanin/*genetics
Spirulina/*metabolism
Cloning, Molecular ; Gene Expression ; Inhibitory Concentration 50 ; Phycocyanin/chemistry ; Phycocyanin/pharmacology ; Plasmids ; Recombinant Proteins/chemistry ; Recombinant Proteins/genetics ; Recombinant Proteins/pharmacology ; Spectrometry, Fluorescence ; Spectrophotometry, Ultraviolet
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknie Pełny tekstSzczegóły
Tytuł:
Plasmonic cell nanocoating: a new concept for rapid microbial screening.
Autorzy:
Xu K; Department of Bioproducts and Biosystems Engineering, University of Minnesota Twin Cities, 2004 Folwell Ave., Saint Paul, MN, 55108-6005, USA.
Bui MN; Department of Bioproducts and Biosystems Engineering, University of Minnesota Twin Cities, 2004 Folwell Ave., Saint Paul, MN, 55108-6005, USA.
Fang A; Department of Bioproducts and Biosystems Engineering, University of Minnesota Twin Cities, 2004 Folwell Ave., Saint Paul, MN, 55108-6005, USA.
Abbas A; Department of Bioproducts and Biosystems Engineering, University of Minnesota Twin Cities, 2004 Folwell Ave., Saint Paul, MN, 55108-6005, USA. .
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Źródło:
Analytical and bioanalytical chemistry [Anal Bioanal Chem] 2017 Nov; Vol. 409 (27), pp. 6305-6314. Date of Electronic Publication: 2017 Sep 13.
Typ publikacji:
Journal Article
MeSH Terms:
Escherichia coli/*isolation & purification
Gold/*chemistry
Metal Nanoparticles/*chemistry
Saccharomyces cerevisiae/*isolation & purification
Chitin/chemistry ; Disulfides/chemistry ; Escherichia coli Infections/microbiology ; Fluorescent Dyes/chemistry ; Humans ; Limit of Detection ; Spectrometry, Fluorescence/methods ; Spectrum Analysis, Raman/methods
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknieSzczegóły
Tytuł:
Rapid fluorescence detection of pathogenic bacteria using magnetic enrichment technique combined with magnetophoretic chromatography.
Autorzy:
Che Y; Defense Key Disciplines Lab of Novel Micro-nano Devices and System Technology, Chongqing University, Chongqing, 400030, China.; School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, 400030, China.
Xu Y; Defense Key Disciplines Lab of Novel Micro-nano Devices and System Technology, Chongqing University, Chongqing, 400030, China. .; Key Laboratory for Optoelectronic Technology & System of Ministry of Education, Chongqing University, Shapingba, Chongqing, 400044, China. .; International R & D Center of Micro-nano Systems and New Materials Technology, Chongqing University, Chongqing, 400030, China. .; School of Optoelectronics Engineering, Chongqing University, Shapingba, Chongqing, 400044, China. .
Wang R; Defense Key Disciplines Lab of Novel Micro-nano Devices and System Technology, Chongqing University, Chongqing, 400030, China.; International R & D Center of Micro-nano Systems and New Materials Technology, Chongqing University, Chongqing, 400030, China.; School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, 400030, China.
Chen L; Defense Key Disciplines Lab of Novel Micro-nano Devices and System Technology, Chongqing University, Chongqing, 400030, China. .; School of Optoelectronics Engineering, Chongqing University, Shapingba, Chongqing, 400044, China. .
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Źródło:
Analytical and bioanalytical chemistry [Anal Bioanal Chem] 2017 Aug; Vol. 409 (20), pp. 4709-4718. Date of Electronic Publication: 2017 Jun 29.
Typ publikacji:
Journal Article
MeSH Terms:
Magnetite Nanoparticles*
Chromatography/*methods
Escherichia coli/*isolation & purification
Colony Count, Microbial ; Limit of Detection ; Reproducibility of Results ; Spectrometry, Fluorescence
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknieSzczegóły
Tytuł:
A rapid and low-cost estimation of bacteria counts in solution using fluorescence spectroscopy.
Autorzy:
Guo R; The Dodd-Walls Centre for Photonic and Quantum Technologies, Department of Physics, The University of Auckland, Private Bag 92019, Auckland, 1142, New Zealand.
McGoverin C; The Dodd-Walls Centre for Photonic and Quantum Technologies, Department of Physics, The University of Auckland, Private Bag 92019, Auckland, 1142, New Zealand.
Swift S; Department of Molecular Medicine and Pathology, The University of Auckland, Private Bag 92019, Auckland, 1142, New Zealand.
Vanholsbeeck F; The Dodd-Walls Centre for Photonic and Quantum Technologies, Department of Physics, The University of Auckland, Private Bag 92019, Auckland, 1142, New Zealand. .
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Źródło:
Analytical and bioanalytical chemistry [Anal Bioanal Chem] 2017 Jun; Vol. 409 (16), pp. 3959-3967. Date of Electronic Publication: 2017 Apr 07.
Typ publikacji:
Journal Article
MeSH Terms:
Acridine Orange/*analysis
Escherichia coli/*isolation & purification
Fluorescent Dyes/*analysis
Spectrometry, Fluorescence/*methods
Bacteria/isolation & purification ; Staining and Labeling/methods
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknieSzczegóły
Tytuł:
Development of an on-site rapid real-time polymerase chain reaction system and the characterization of suitable DNA polymerases for TaqMan probe technology.
Autorzy:
Furutani S; Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-8-31 Midorigaoka, Ikeda, Osaka, 563-8577, Japan.
Naruishi N; Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-8-31 Midorigaoka, Ikeda, Osaka, 563-8577, Japan.
Hagihara Y; Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-8-31 Midorigaoka, Ikeda, Osaka, 563-8577, Japan.
Nagai H; Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-8-31 Midorigaoka, Ikeda, Osaka, 563-8577, Japan. .
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Źródło:
Analytical and bioanalytical chemistry [Anal Bioanal Chem] 2016 Aug; Vol. 408 (20), pp. 5641-9. Date of Electronic Publication: 2016 Jun 07.
Typ publikacji:
Journal Article
MeSH Terms:
DNA, Bacterial/*genetics
Escherichia coli/*genetics
Escherichia coli/*isolation & purification
Real-Time Polymerase Chain Reaction/*methods
Spectrometry, Fluorescence/*methods
Taq Polymerase/*genetics
Reproducibility of Results ; Sensitivity and Specificity
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknieSzczegóły
Tytuł:
Destabilizing an interacting motif strengthens the association of a designed ankyrin repeat protein with tubulin.
Autorzy:
Ahmad S; Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ Paris-Sud, Université Paris-Saclay, 91198, Gif-sur-Yvette cedex, France.
Pecqueur L; Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ Paris-Sud, Université Paris-Saclay, 91198, Gif-sur-Yvette cedex, France.
Dreier B; Department of Biochemistry, University of Zurich, Winterthurerstrasse 190, CH-8057, Zurich, Switzerland.
Hamdane D; Laboratoire de Chimie des Processus Biologiques, CNRS-UMR 8229, Collège De France, 11 place Marcelin Berthelot, 75231 Paris Cedex 05, France.
Aumont-Nicaise M; Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ Paris-Sud, Université Paris-Saclay, 91198, Gif-sur-Yvette cedex, France.
Plückthun A; Department of Biochemistry, University of Zurich, Winterthurerstrasse 190, CH-8057, Zurich, Switzerland.
Knossow M; Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ Paris-Sud, Université Paris-Saclay, 91198, Gif-sur-Yvette cedex, France.
Gigant B; Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ Paris-Sud, Université Paris-Saclay, 91198, Gif-sur-Yvette cedex, France.
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Źródło:
Scientific reports [Sci Rep] 2016 Jul 06; Vol. 6, pp. 28922. Date of Electronic Publication: 2016 Jul 06.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
Ankyrin Repeat*
Escherichia coli/*metabolism
Escherichia coli Proteins/*chemistry
Tubulin/*chemistry
Amino Acid Motifs ; Ankyrins/chemistry ; Circular Dichroism ; Cloning, Molecular ; Enzyme-Linked Immunosorbent Assay ; Kinetics ; Models, Molecular ; Mutagenesis ; Mutation ; Protein Binding ; Protein Engineering ; Ribosomes/chemistry ; Spectrometry, Fluorescence ; Surface Plasmon Resonance
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknieSzczegóły
Tytuł:
Conformational change in individual enzyme molecules.
Autorzy:
Crawford JJ; a Department of Chemistry, 360 Parker Building, University of Manitoba, Winnipeg, MB R3T 2N2, Canada.
Itzkow F; b Department of Chemistry, 599 Portage Avenue, University of Winnipeg, Winnipeg, MB R3B 2G3, Canada.
MacLean J; b Department of Chemistry, 599 Portage Avenue, University of Winnipeg, Winnipeg, MB R3B 2G3, Canada.
Craig DB; b Department of Chemistry, 599 Portage Avenue, University of Winnipeg, Winnipeg, MB R3B 2G3, Canada.
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Źródło:
Biochemistry and cell biology = Biochimie et biologie cellulaire [Biochem Cell Biol] 2015 Dec; Vol. 93 (6), pp. 611-8. Date of Electronic Publication: 2015 Sep 30.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
Models, Molecular*
Escherichia coli/*enzymology
Escherichia coli Proteins/*chemistry
beta-Galactosidase/*chemistry
Acridines/chemistry ; Acridines/metabolism ; Electrophoresis, Capillary ; Electrophoretic Mobility Shift Assay ; Enzyme Stability ; Escherichia coli/genetics ; Escherichia coli/growth & development ; Escherichia coli Proteins/genetics ; Escherichia coli Proteins/isolation & purification ; Escherichia coli Proteins/metabolism ; Fluorescent Dyes/chemistry ; Fluorescent Dyes/metabolism ; Galactose/analogs & derivatives ; Galactose/chemistry ; Galactose/metabolism ; Galactosides/chemistry ; Galactosides/metabolism ; Gene Deletion ; Heat-Shock Proteins/genetics ; Heat-Shock Proteins/metabolism ; Heat-Shock Response ; Hot Temperature/adverse effects ; Lac Operon ; Protein Conformation ; Protein Folding ; Recombinant Proteins/chemistry ; Recombinant Proteins/isolation & purification ; Recombinant Proteins/metabolism ; Reproducibility of Results ; Spectrometry, Fluorescence ; Stochastic Processes ; beta-Galactosidase/genetics ; beta-Galactosidase/isolation & purification ; beta-Galactosidase/metabolism
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknieSzczegóły
Tytuł:
Identification of a Cryptic Bacterial Promoter in Mouse (mdr1a) P-Glycoprotein cDNA.
Autorzy:
Pluchino KM; Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, United States of America.
Esposito D; Protein Expression Laboratory, Frederick National Laboratory for Cancer Research, Leidos Biomedical Research, Inc., Frederick, MD, United States of America.
Moen JK; Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, United States of America.
Hall MD; Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, United States of America.
Madigan JP; Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, United States of America.
Shukla S; Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, United States of America.
Procter LV; Protein Expression Laboratory, Frederick National Laboratory for Cancer Research, Leidos Biomedical Research, Inc., Frederick, MD, United States of America.
Wall VE; Protein Expression Laboratory, Frederick National Laboratory for Cancer Research, Leidos Biomedical Research, Inc., Frederick, MD, United States of America.
Schneider TD; Gene Regulation and Chromosome Biology Laboratory, National Cancer Institute, Molecular Information Theory Group, Frederick, MD, United States of America.
Pringle I; Gene Medicine Research Group, NDCLS, Radcliffe Department of Medicine, University of Oxford, Oxford, United Kingdom.
Ambudkar SV; Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, United States of America.
Gill DR; Gene Medicine Research Group, NDCLS, Radcliffe Department of Medicine, University of Oxford, Oxford, United Kingdom.
Hyde SC; Gene Medicine Research Group, NDCLS, Radcliffe Department of Medicine, University of Oxford, Oxford, United Kingdom.
Gottesman MM; Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, United States of America.
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Źródło:
PloS one [PLoS One] 2015 Aug 26; Vol. 10 (8), pp. e0136396. Date of Electronic Publication: 2015 Aug 26 (Print Publication: 2015).
Typ publikacji:
Journal Article; Research Support, N.I.H., Extramural; Research Support, N.I.H., Intramural
MeSH Terms:
ATP Binding Cassette Transporter, Subfamily B/*genetics
DNA, Complementary/*genetics
Escherichia coli/*growth & development
Promoter Regions, Genetic/*genetics
ATP Binding Cassette Transporter, Subfamily B/metabolism ; Animals ; Binding Sites ; Blotting, Western ; DNA-Directed RNA Polymerases/metabolism ; Escherichia coli/genetics ; Escherichia coli/metabolism ; Flow Cytometry ; HEK293 Cells ; Humans ; Mice ; Microscopy, Confocal ; Plasmids/genetics ; Protein Conformation ; Sigma Factor/metabolism ; Spectrometry, Fluorescence
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknieSzczegóły
Tytuł:
Quantitative Brightness Analysis of Fluorescence Intensity Fluctuations in E. Coli.
Autorzy:
Hur KH; School of Physics and Astronomy, University of Minnesota, Minneapolis, Minnesota, United States of America.
Mueller JD; School of Physics and Astronomy, University of Minnesota, Minneapolis, Minnesota, United States of America; Department of Biomedical Engineering, University of Minnesota, Minneapolis, Minnesota, United States of America.
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Źródło:
PloS one [PLoS One] 2015 Jun 22; Vol. 10 (6), pp. e0130063. Date of Electronic Publication: 2015 Jun 22 (Print Publication: 2015).
Typ publikacji:
Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.
MeSH Terms:
Escherichia coli/*physiology
Green Fluorescent Proteins/*metabolism
Nucleocytoplasmic Transport Proteins/*physiology
Staining and Labeling/*methods
Algorithms ; Cell Line ; Fluorescence ; Humans ; Models, Theoretical ; Photobleaching ; Protein Binding ; Spectrometry, Fluorescence
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknieSzczegóły
Tytuł:
Production of an antimicrobial peptide AN5-1 in Escherichia coli and its dual mechanisms against bacteria.
Autorzy:
Yi T; Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, Jilin University, 2699 Qianjin Street, Changchun, Jilin, 130012, China; School of Life Sciences, Jilin University, Changchun, Jilin, 130012, China.
Huang Y
Chen Y
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Źródło:
Chemical biology & drug design [Chem Biol Drug Des] 2015 May; Vol. 85 (5), pp. 598-607. Date of Electronic Publication: 2014 Nov 04.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
Anti-Infective Agents/*metabolism
Anti-Infective Agents/*pharmacology
Antimicrobial Cationic Peptides/*metabolism
Antimicrobial Cationic Peptides/*pharmacology
Bacteria/*drug effects
Escherichia coli/*metabolism
Amino Acid Sequence ; Anti-Infective Agents/chemistry ; Antimicrobial Cationic Peptides/chemistry ; Bacteria/metabolism ; Cell Wall/drug effects ; Circular Dichroism ; DNA/chemistry ; DNA/metabolism ; Erythrocytes/drug effects ; Erythrocytes/metabolism ; Escherichia coli/drug effects ; Hemolysis/drug effects ; Humans ; Microbial Sensitivity Tests ; Paenibacillus/metabolism ; Protein Binding ; Protein Structure, Secondary ; Recombinant Fusion Proteins/biosynthesis ; Recombinant Fusion Proteins/chemistry ; Recombinant Fusion Proteins/isolation & purification ; Recombinant Fusion Proteins/pharmacology ; Spectrometry, Fluorescence
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknieSzczegóły
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