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Tytuł :
Lipoic Acid-Modified Oligoethyleneimine-Mediated miR-34a Delivery to Achieve the Anti-Tumor Efficacy.
Autorzy :
Huang Y; Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, School of Life Sciences, Jilin University, Changchun 130012, China.
Wang L; Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, School of Life Sciences, Jilin University, Changchun 130012, China.
Chen Y; Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, School of Life Sciences, Jilin University, Changchun 130012, China.
Han H; Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, School of Life Sciences, Jilin University, Changchun 130012, China.
Li Q; Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, School of Life Sciences, Jilin University, Changchun 130012, China.
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Źródło :
Molecules (Basel, Switzerland) [Molecules] 2021 Aug 10; Vol. 26 (16). Date of Electronic Publication: 2021 Aug 10.
Typ publikacji :
Journal Article
MeSH Terms :
Transfection*
Antineoplastic Agents/*pharmacology
MicroRNAs/*metabolism
Polyethyleneimine/*chemistry
Thioctic Acid/*chemistry
Apoptosis/drug effects ; Cell Cycle Checkpoints/drug effects ; Cell Movement/drug effects ; Cell Proliferation/drug effects ; HeLa Cells ; Humans ; MicroRNAs/genetics ; Nanoparticles/ultrastructure ; Polyethyleneimine/chemical synthesis ; Thioctic Acid/chemical synthesis ; Wound Healing/drug effects
Czasopismo naukowe
Tytuł :
Nanotechnology-Based Strategies to Overcome Current Barriers in Gene Delivery.
Autorzy :
Mirón-Barroso S; Institute of Molecular Biology of Barcelona, IBMB-CSIC, 08028 Barcelona, Spain.
Domènech EB; Department of Genetics Microbiology and Statistics, University of Barcelona, 08007 Barcelona, Spain.
Trigueros S; Institute of Molecular Biology of Barcelona, IBMB-CSIC, 08028 Barcelona, Spain.; Department of Genetics Microbiology and Statistics, University of Barcelona, 08007 Barcelona, Spain.; Department of Zoology, University of Oxford, Oxford OX1 3PS, UK.
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Źródło :
International journal of molecular sciences [Int J Mol Sci] 2021 Aug 09; Vol. 22 (16). Date of Electronic Publication: 2021 Aug 09.
Typ publikacji :
Journal Article; Review
MeSH Terms :
Genetic Therapy*
Nanotechnology*
Transfection*
Nanostructures/*therapeutic use
Animals ; Humans
Czasopismo naukowe
Tytuł :
Mutational burden, MHC-I expression and immune infiltration as limiting factors for in situ vaccination by TNFα and IL-12 gene electrotransfer.
Autorzy :
Kamensek U; Department of Experimental Oncology, Institute of Oncology Ljubljana, Zaloska cesta 2, SI-1000 Ljubljana, Slovenia; University of Ljubljana, Biotechnical Faculty, Jamnikarjeva ulica 101, SI-1000 Ljubljana, Slovenia. Electronic address: .
Ursic K; Department of Experimental Oncology, Institute of Oncology Ljubljana, Zaloska cesta 2, SI-1000 Ljubljana, Slovenia; University of Ljubljana, Biotechnical Faculty, Jamnikarjeva ulica 101, SI-1000 Ljubljana, Slovenia.
Markelc B; Department of Experimental Oncology, Institute of Oncology Ljubljana, Zaloska cesta 2, SI-1000 Ljubljana, Slovenia; Faculty of Health Sciences, University of Ljubljana, Zdravstvena pot 5, SI-1000 Ljubljana, Slovenia.
Cemazar M; Department of Experimental Oncology, Institute of Oncology Ljubljana, Zaloska cesta 2, SI-1000 Ljubljana, Slovenia; Faculty of Health Sciences, University of Primorska, Polje 42, SI-6310 Izola, Slovenia.
Setrajcic Dragos V; Department of Molecular Diagnostics, Institute of Oncology Ljubljana, Zaloska cesta 2, SI-1000 Ljubljana, Slovenia.
Sersa G; Department of Experimental Oncology, Institute of Oncology Ljubljana, Zaloska cesta 2, SI-1000 Ljubljana, Slovenia; Faculty of Health Sciences, University of Ljubljana, Zdravstvena pot 5, SI-1000 Ljubljana, Slovenia.
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Źródło :
Bioelectrochemistry (Amsterdam, Netherlands) [Bioelectrochemistry] 2021 Aug; Vol. 140, pp. 107831. Date of Electronic Publication: 2021 Apr 28.
Typ publikacji :
Journal Article
MeSH Terms :
Mutation*
Transfection*
Vaccination*
Gene Expression Regulation/*immunology
Histocompatibility Antigens Class I/*genetics
Interleukin-12/*genetics
Tumor Necrosis Factor-alpha/*genetics
Animals ; Cell Line, Tumor ; Mice
Czasopismo naukowe
Tytuł :
Exosomes of Mesenchymal Stem Cells as a Proper Vehicle for Transfecting miR-145 into the Breast Cancer Cell Line and Its Effect on Metastasis.
Autorzy :
Sheykhhasan M; Department of Mesenchymal Stem Cells, Academic Center for Education, Culture and Research (ACECR), Qom Branch, Qom, Iran.
Kalhor N; Department of Mesenchymal Stem Cells, Academic Center for Education, Culture and Research (ACECR), Qom Branch, Qom, Iran.
Sheikholeslami A; Department of Mesenchymal Stem Cells, Academic Center for Education, Culture and Research (ACECR), Qom Branch, Qom, Iran.
Dolati M; Cellular and Molecular Research Center, Qom University of Medical Sciences, Qom, Iran.
Amini E; Department of Cellular & Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran.
Fazaeli H; Department of Mesenchymal Stem Cells, Academic Center for Education, Culture and Research (ACECR), Qom Branch, Qom, Iran.
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Źródło :
BioMed research international [Biomed Res Int] 2021 Jun 29; Vol. 2021, pp. 5516078. Date of Electronic Publication: 2021 Jun 29 (Print Publication: 2021).
Typ publikacji :
Journal Article
MeSH Terms :
Transfection*
Breast Neoplasms/*genetics
Breast Neoplasms/*pathology
Exosomes/*metabolism
Mesenchymal Stem Cells/*metabolism
MicroRNAs/*metabolism
Adipose Tissue/cytology ; Breast Neoplasms/enzymology ; Cell Line, Tumor ; Exosomes/ultrastructure ; Female ; Gene Expression Regulation, Neoplastic ; Humans ; Matrix Metalloproteinase 9/genetics ; Matrix Metalloproteinase 9/metabolism ; MicroRNAs/genetics ; Neoplasm Metastasis ; Receptor, ErbB-2/metabolism ; Tumor Suppressor Protein p53/genetics ; Tumor Suppressor Protein p53/metabolism ; rho-Associated Kinases/genetics ; rho-Associated Kinases/metabolism
Czasopismo naukowe
Tytuł :
Efficient and gentle delivery of molecules into cells with different elasticity via Progressive Mechanoporation.
Autorzy :
Uvizl A; Cell Cycle, Biotechnology Center, Technische Universität Dresden, 01307 Dresden, Germany.
Goswami R; Max Planck Institute for the Science of Light & Max-Planck-Zentrum für Physik und Medizin, 91058 Erlangen, Germany. .
Gandhi SD; Cell Cycle, Biotechnology Center, Technische Universität Dresden, 01307 Dresden, Germany.
Augsburg M; Medical Systems Biology, Medical Faculty and University Hospital Carl Gustav Carus, TU Dresden, 01307 Dresden, Germany.
Buchholz F; Medical Systems Biology, Medical Faculty and University Hospital Carl Gustav Carus, TU Dresden, 01307 Dresden, Germany.
Guck J; Max Planck Institute for the Science of Light & Max-Planck-Zentrum für Physik und Medizin, 91058 Erlangen, Germany. .
Mansfeld J; Cell Cycle, Biotechnology Center, Technische Universität Dresden, 01307 Dresden, Germany and The Institute of Cancer Research, London SW7 3RP, UK. .
Girardo S; Max Planck Institute for the Science of Light & Max-Planck-Zentrum für Physik und Medizin, 91058 Erlangen, Germany. .
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Źródło :
Lab on a chip [Lab Chip] 2021 Jun 15; Vol. 21 (12), pp. 2437-2452.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Transfection*
Cell Membrane ; Cell Membrane Permeability ; Elasticity ; Stress, Mechanical
Czasopismo naukowe
Tytuł :
Regulation of gene transfection by cell size, shape and elongation on micropatterned surfaces.
Autorzy :
Wang Y; Research Center for Functional Materials, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan. , Graduate School of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8577, Japan.
Yang Y; Research Center for Functional Materials, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan. , Graduate School of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8577, Japan.
Yoshitomi T; Research Center for Functional Materials, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan. .
Kawazoe N; Research Center for Functional Materials, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan. .
Yang Y; Graduate School of Life and Environmental Science, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan.
Chen G; Research Center for Functional Materials, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan. , Graduate School of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8577, Japan.
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Źródło :
Journal of materials chemistry. B [J Mater Chem B] 2021 Jun 03; Vol. 9 (21), pp. 4329-4339.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Cell Shape*
Cell Size*
Transfection*
Mesenchymal Stem Cells/*cytology
Actin Cytoskeleton/metabolism ; Cell Nucleus/metabolism ; Cytoskeleton/metabolism ; DNA/biosynthesis ; Fluorescent Dyes/chemistry ; Humans ; Mesenchymal Stem Cells/metabolism ; Microscopy, Fluorescence ; Surface Properties
Czasopismo naukowe
Tytuł :
Development of Covalent Chitosan-Polyethylenimine Derivatives as Gene Delivery Vehicle: Synthesis, Characterization, and Evaluation.
Autorzy :
Nicolle L; Group for Functionalized Biomaterials, Institute of Chemical Sciences and Engineering Ecole Polytechnique Fédérale de Lausanne, EPFL SB ISIC SCI-SB-SG, Station 6, CH-1015 Lausanne, Switzerland.
Casper J; Division of Pharmaceutical Technology, Department of Pharmaceutical Sciences, University of Basel, Klingelbergstrasse 50/70, CH-4056 Basel, Switzerland.
Willimann M; Division of Metabolism and Children's Research Center, University Children's Hospital Zürich, CH-8032 Zürich, Switzerland.
Journot CMA; Group for Functionalized Biomaterials, Institute of Chemical Sciences and Engineering Ecole Polytechnique Fédérale de Lausanne, EPFL SB ISIC SCI-SB-SG, Station 6, CH-1015 Lausanne, Switzerland.
Detampel P; Division of Pharmaceutical Technology, Department of Pharmaceutical Sciences, University of Basel, Klingelbergstrasse 50/70, CH-4056 Basel, Switzerland.
Einfalt T; Division of Pharmaceutical Technology, Department of Pharmaceutical Sciences, University of Basel, Klingelbergstrasse 50/70, CH-4056 Basel, Switzerland.
Grisch-Chan HM; Division of Metabolism and Children's Research Center, University Children's Hospital Zürich, CH-8032 Zürich, Switzerland.
Thöny B; Division of Metabolism and Children's Research Center, University Children's Hospital Zürich, CH-8032 Zürich, Switzerland.
Gerber-Lemaire S; Group for Functionalized Biomaterials, Institute of Chemical Sciences and Engineering Ecole Polytechnique Fédérale de Lausanne, EPFL SB ISIC SCI-SB-SG, Station 6, CH-1015 Lausanne, Switzerland.
Huwyler J; Division of Pharmaceutical Technology, Department of Pharmaceutical Sciences, University of Basel, Klingelbergstrasse 50/70, CH-4056 Basel, Switzerland.
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Źródło :
International journal of molecular sciences [Int J Mol Sci] 2021 Apr 07; Vol. 22 (8). Date of Electronic Publication: 2021 Apr 07.
Typ publikacji :
Journal Article
MeSH Terms :
Gene Transfer Techniques*
Transfection*/methods
Chitosan/*chemistry
Polyethyleneimine/*chemistry
Animals ; Cell Line, Tumor ; Cell Survival ; Chemical Phenomena ; Chemistry Techniques, Synthetic ; Colloids/chemistry ; DNA/chemistry ; Gene Expression ; Genes, Reporter ; Genetic Vectors ; Humans ; Magnetic Resonance Spectroscopy ; Mice
Czasopismo naukowe
Tytuł :
Polypurine Reverse-Hoogsteen Hairpins as a Tool for Exon Skipping at the Genomic Level in Mammalian Cells.
Autorzy :
Noé V; Department of Biochemistry and Physiology, School of Pharmacy and Food Sciences & IN2UB, University of Barcelona, 08028 Barcelona, Spain.
Ciudad CJ; Department of Biochemistry and Physiology, School of Pharmacy and Food Sciences & IN2UB, University of Barcelona, 08028 Barcelona, Spain.
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Źródło :
International journal of molecular sciences [Int J Mol Sci] 2021 Apr 06; Vol. 22 (7). Date of Electronic Publication: 2021 Apr 06.
Typ publikacji :
Journal Article
MeSH Terms :
Exons*
Inverted Repeat Sequences*
RNA, Messenger*/genetics
RNA, Messenger*/metabolism
Tetrahydrofolate Dehydrogenase*/biosynthesis
Tetrahydrofolate Dehydrogenase*/genetics
Transfection*
Animals ; Cell Line, Tumor ; Cricetulus ; Female
Czasopismo naukowe
Tytuł :
Concurrent transfection of randomized transgene configurations into targeted integration CHO host is an advantageous and cost-effective method for expression of complex molecules.
Autorzy :
Dong E; Cell Culture and Bioprocess Operations Department, Genentech, Inc. 1 DNA Way, South San Francisco, California, USA.
Lam C; Cell Culture and Bioprocess Operations Department, Genentech, Inc. 1 DNA Way, South San Francisco, California, USA.
Tang D; Cell Culture and Bioprocess Operations Department, Genentech, Inc. 1 DNA Way, South San Francisco, California, USA.
Louie S; Cell Culture and Bioprocess Operations Department, Genentech, Inc. 1 DNA Way, South San Francisco, California, USA.
Yim M; Cell Culture and Bioprocess Operations Department, Genentech, Inc. 1 DNA Way, South San Francisco, California, USA.
Williams AJ; Purification Development Department, Genentech, Inc. 1 DNA Way, South San Francisco, California, USA.
Sawyer W; Biochemical and Cellular Pharmacology Department, Genentech, Inc. 1 DNA Way, South San Francisco, California, USA.
Yip S; Cell Culture and Bioprocess Operations Department, Genentech, Inc. 1 DNA Way, South San Francisco, California, USA.
Carver J; Cell Culture and Bioprocess Operations Department, Genentech, Inc. 1 DNA Way, South San Francisco, California, USA.
AlBarakat A; Cell Culture and Bioprocess Operations Department, Genentech, Inc. 1 DNA Way, South San Francisco, California, USA.
Tsukuda J; Cell Culture and Bioprocess Operations Department, Genentech, Inc. 1 DNA Way, South San Francisco, California, USA.
Snedecor B; Cell Culture and Bioprocess Operations Department, Genentech, Inc. 1 DNA Way, South San Francisco, California, USA.
Misaghi S; Cell Culture and Bioprocess Operations Department, Genentech, Inc. 1 DNA Way, South San Francisco, California, USA.
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Źródło :
Biotechnology journal [Biotechnol J] 2021 Apr; Vol. 16 (4), pp. e2000230. Date of Electronic Publication: 2020 Dec 30.
Typ publikacji :
Journal Article
MeSH Terms :
Transfection*
Transgenes*/genetics
Animals ; CHO Cells ; Cost-Benefit Analysis ; Cricetinae ; Cricetulus ; Recombinant Proteins/genetics
Czasopismo naukowe
Tytuł :
Evaluation of a Novel Plasmid for Simultaneous Gene Electrotransfer-Mediated Silencing of CD105 and CD146 in Combination with Irradiation.
Autorzy :
Savarin M; Department of Experimental Oncology, Institute of Oncology Ljubljana, 1000 Ljubljana, Slovenia.
Kamensek U; Department of Experimental Oncology, Institute of Oncology Ljubljana, 1000 Ljubljana, Slovenia.; Biotechnical Faculty, University of Ljubljana, 1000 Ljubljana, Slovenia.
Znidar K; Department of Experimental Oncology, Institute of Oncology Ljubljana, 1000 Ljubljana, Slovenia.
Todorovic V; Department of Experimental Oncology, Institute of Oncology Ljubljana, 1000 Ljubljana, Slovenia.
Sersa G; Department of Experimental Oncology, Institute of Oncology Ljubljana, 1000 Ljubljana, Slovenia.; Faculty of Health Sciences, University of Ljubljana, 1000 Ljubljana, Slovenia.
Cemazar M; Department of Experimental Oncology, Institute of Oncology Ljubljana, 1000 Ljubljana, Slovenia.; Faculty of Health Sciences, University of Primorska, 6310 Izola, Slovenia.
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Źródło :
International journal of molecular sciences [Int J Mol Sci] 2021 Mar 17; Vol. 22 (6). Date of Electronic Publication: 2021 Mar 17.
Typ publikacji :
Journal Article
MeSH Terms :
Electroporation*
Gene Silencing*
Radiation, Ionizing*
Transfection*
CD146 Antigen/*genetics
Endoglin/*genetics
Plasmids/*genetics
Animals ; Cell Death ; Cell Line ; Cytokines/metabolism ; Endothelial Cells/radiation effects ; Membrane Proteins ; Mice ; Neovascularization, Physiologic/radiation effects
Czasopismo naukowe
Tytuł :
mRNA Transfection of T-Lymphocytes by Electroporation.
Autorzy :
Schwarze LI; Research Department of Cell and Gene Therapy, Department of Stem Cell Transplantation, University Medical Center Hamburg-Eppendorf, Hamburg, Germany. .; German Center for Infection Research (DZIF), Hamburg, Germany. .
Fehse B; Research Department of Cell and Gene Therapy, Department of Stem Cell Transplantation, University Medical Center Hamburg-Eppendorf, Hamburg, Germany. .; German Center for Infection Research (DZIF), Hamburg, Germany. .
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Źródło :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2285, pp. 217-226.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Electroporation*
Transfection*
CD4-Positive T-Lymphocytes/*metabolism
RNA, Messenger/*metabolism
Animals ; CD4-Positive T-Lymphocytes/immunology ; Cells, Cultured ; Gene Knockout Techniques ; Humans ; RNA, Messenger/genetics ; Receptors, CCR5/genetics ; Receptors, CCR5/metabolism ; Research Design ; Transcription Activator-Like Effector Nucleases/genetics ; Transcription Activator-Like Effector Nucleases/metabolism ; Workflow
Czasopismo naukowe
Tytuł :
siRNA Delivery to Melanoma Cells with Cationic Niosomes.
Autorzy :
Obeid MA; Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, Yarmouk University, Irbid, Jordan. .
Alyamani H; Strathclyde Institute of Pharmacy & Biomedical Sciences, University of Strathclyde, Glasgow, UK.
Amawi H; Department of Pharmacy Practice, Faculty of Pharmacy, Yarmouk University, Irbid, Jordan.
Aljabali AAA; Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, Yarmouk University, Irbid, Jordan.
Rezigue M; Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, Yarmouk University, Irbid, Jordan.
Abdeljaber SN; Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, Yarmouk University, Irbid, Jordan.
Ferro VA; Strathclyde Institute of Pharmacy & Biomedical Sciences, University of Strathclyde, Glasgow, UK.
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Źródło :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2265, pp. 621-634.
Typ publikacji :
Journal Article
MeSH Terms :
Melanoma*/genetics
Melanoma*/metabolism
Melanoma*/pathology
Melanoma*/therapy
Nanoparticles*/chemistry
Nanoparticles*/therapeutic use
RNA, Small Interfering*/chemistry
RNA, Small Interfering*/pharmacology
Transfection*
Humans ; Liposomes
Czasopismo naukowe
Tytuł :
Transfection of Neuronal Cultures.
Autorzy :
Sariyer IK; Department of Neuroscience, Lewis Katz School of Medicine, Temple University, Philadelphia, PA, USA. .
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Źródło :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2311, pp. 147-153.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural
MeSH Terms :
Transfection*
Lipids/*chemistry
Neurons/*physiology
Animals ; Cells, Cultured ; Gene Expression Regulation ; Humans ; Neurons/metabolism ; Primary Cell Culture
Czasopismo naukowe
Tytuł :
MRI-Visible Nanovehicle for Efficient siRNA Delivery.
Autorzy :
Jin R; National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, China.
Zhu W; Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, China.
Lin G; National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, China.
Liu G; Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University, Xiamen, China.
Ai H; National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, China. .; Department of Radiology, West China Hospital, Sichuan University, Chengdu, China. .
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Źródło :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2282, pp. 195-208.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Magnetic Resonance Imaging*
RNA Interference*
Transfection*
Breast Neoplasms/*diagnostic imaging
Contrast Media/*metabolism
Ferric Compounds/*metabolism
Polyethyleneimine/*chemistry
RNA, Small Interfering/*genetics
Animals ; Breast Neoplasms/genetics ; Breast Neoplasms/metabolism ; Contrast Media/chemistry ; Female ; Ferric Compounds/chemistry ; Gene Expression Regulation, Neoplastic ; Humans ; MCF-7 Cells ; Mice ; RNA, Small Interfering/chemistry ; RNA, Small Interfering/metabolism ; Research Design ; Workflow
Czasopismo naukowe
Tytuł :
High-Throughput Expression Screening in Mammalian Suspension Cells.
Autorzy :
Chapple SD; IONTAS Ltd, Unit 2, Iconix Park, London Road, Pampisford, Cambridge, UK.
Dyson MR; Department of Antibody Engineering, Ichnos Sciences S.A., Biopôle Lausanne-Epalinges, Epalinges, Switzerland. .
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Źródło :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2199, pp. 117-125.
Typ publikacji :
Journal Article
MeSH Terms :
Cloning, Molecular*
Gene Expression*
High-Throughput Screening Assays*
Recombinant Proteins*/biosynthesis
Recombinant Proteins*/genetics
Transfection*
Genetic Vectors/*genetics
HEK293 Cells ; Humans
Czasopismo naukowe
Tytuł :
β-arrestin 1 transfection induced cell death in high grade glioma in vitro .
Autorzy :
Folcuti C; Department of Biochemistry, University of Medicine and Pharmacy of Craiova , Craiova, Romania.
Horescu C; Department of Biochemistry, University of Medicine and Pharmacy of Craiova , Craiova, Romania.
Barcan E; Department of Biochemistry, University of Medicine and Pharmacy of Craiova , Craiova, Romania.
Alexandru O; Department of Neurology, University of Medicine and Pharmacy of Craiova , Craiova, Romania.
Tuta C; Department of Biochemistry, University of Medicine and Pharmacy of Craiova , Craiova, Romania.
Vatu BI; Department of Biochemistry, University of Medicine and Pharmacy of Craiova , Craiova, Romania.
Artene SA; Department of Biochemistry, University of Medicine and Pharmacy of Craiova , Craiova, Romania.
Dricu A; Department of Biochemistry, University of Medicine and Pharmacy of Craiova , Craiova, Romania.
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Źródło :
Journal of immunoassay & immunochemistry [J Immunoassay Immunochem] 2020 Nov 01; Vol. 41 (6), pp. 1021-1032. Date of Electronic Publication: 2020 Aug 18.
Typ publikacji :
Journal Article
MeSH Terms :
Transfection*
Cell Death/*genetics
Glioma/*therapy
beta-Arrestin 1/*genetics
beta-Arrestin 1/*metabolism
Antineoplastic Agents, Alkylating/pharmacology ; Cell Death/drug effects ; Cell Proliferation/drug effects ; Cell Survival/drug effects ; Dose-Response Relationship, Drug ; Drug Screening Assays, Antitumor ; Glioma/metabolism ; Glioma/pathology ; Humans ; Structure-Activity Relationship ; Temozolomide/pharmacology ; Tumor Cells, Cultured
Czasopismo naukowe
Tytuł :
Sonogenetic Modulation of Cellular Activities in Mammalian Cells.
Autorzy :
Huang YS; Institute of Molecular Medicine, National Tsing Hua University, Hsinchu, Taiwan.
Fan CH; Department of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, Hsinchu, Taiwan.
Yang WT; Institute of Molecular Medicine, National Tsing Hua University, Hsinchu, Taiwan.
Yeh CK; Department of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, Hsinchu, Taiwan. .
Lin YC; Institute of Molecular Medicine, National Tsing Hua University, Hsinchu, Taiwan. .; Department of Molecular Medicine, National Tsing Hua University, Hsinchu, Taiwan. .
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Źródło :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2312, pp. 109-124.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Cell Engineering*
Transfection*
Ultrasonics*
Brain/*metabolism
Molecular Motor Proteins/*genetics
Animals ; Cell Culture Techniques ; Gene Expression Regulation ; Genetic Vectors ; HEK293 Cells ; Humans ; Mice, Inbred C57BL ; Microbubbles ; Molecular Motor Proteins/metabolism ; Mutation
Czasopismo naukowe
Tytuł :
Fundamental and Practical Aspects in the Formulation of Colloidal Polyelectrolyte Complexes of Chitosan and siRNA.
Autorzy :
Schatz C; Laboratoire de Chimie des Polymères Organiques (LCPO), University of Bordeaux, CNRS, Bordeaux INP, UMR 5629, Pessac, France.
Delas T; Laboratoire de Chimie des Polymères Organiques (LCPO), University of Bordeaux, CNRS, Bordeaux INP, UMR 5629, Pessac, France. .
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Źródło :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2282, pp. 297-327.
Typ publikacji :
Journal Article; Review
MeSH Terms :
RNA Interference*
Transfection*
Chitosan/*chemistry
Polyelectrolytes/*chemistry
RNA, Small Interfering/*genetics
Animals ; Cell Line ; Colloids ; Humans ; RNA, Small Interfering/chemistry ; RNA, Small Interfering/metabolism ; Static Electricity
Czasopismo naukowe
Tytuł :
Isolation of Primary Human and Rodent Brain Microvascular Endothelial Cells: Culturing, Characterization, and High-Efficiency Transfection.
Autorzy :
Darbinian N; Department of Neurology, Lewis Katz School of Medicine, Temple University, Philadelphia, PA, USA. .; Shriners Hospital Pediatric Research Center, Temple University School of Medicine, Philadelphia, PA, USA. .
Darbinyan A; Department of Pathology, Yale University School of Medicine, New Haven, CT, USA.
Merabova N; Shriners Hospital Pediatric Research Center, Temple University School of Medicine, Philadelphia, PA, USA.
Amini S; Department of Biology, College of Science & Technology, Temple University, Philadelphia, PA, USA.
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Źródło :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2311, pp. 185-193.
Typ publikacji :
Journal Article
MeSH Terms :
Cell Separation*
Transfection*
Blood-Brain Barrier/*physiology
Brain/*blood supply
Endothelial Cells/*physiology
Microvessels/*cytology
Animals ; Blood-Brain Barrier/metabolism ; Cell Culture Techniques ; Cell Differentiation ; Cell Proliferation ; Cells, Cultured ; Endothelial Cells/metabolism ; Fetus ; Gestational Age ; Humans ; Mice ; Rats
Czasopismo naukowe
Tytuł :
Cationic Dendrimers for siRNA Delivery: An Overview of Methods for In Vitro/In Vivo Characterization.
Autorzy :
Laurini E; Molecular Biology and Nanotechnology Laboratory (), Department of Engineering and Architecture, University of Trieste, Trieste, Italy. .
Aulic S; Molecular Biology and Nanotechnology Laboratory (), Department of Engineering and Architecture, University of Trieste, Trieste, Italy.
Marson D; Molecular Biology and Nanotechnology Laboratory (), Department of Engineering and Architecture, University of Trieste, Trieste, Italy.
Fermeglia M; Molecular Biology and Nanotechnology Laboratory (), Department of Engineering and Architecture, University of Trieste, Trieste, Italy.
Pricl S; Molecular Biology and Nanotechnology Laboratory (), Department of Engineering and Architecture, University of Trieste, Trieste, Italy.; Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Poland.
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Źródło :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2282, pp. 209-244.
Typ publikacji :
Journal Article; Review
MeSH Terms :
RNA Interference*
RNAi Therapeutics*
Transfection*
Dendrimers/*chemistry
RNA, Small Interfering/*genetics
Animals ; Cations ; Cell Line ; Dendrimers/metabolism ; Dendrimers/toxicity ; Humans ; RNA, Small Interfering/chemistry ; RNA, Small Interfering/metabolism
Czasopismo naukowe

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