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Tytuł :
Pogz deficiency leads to transcription dysregulation and impaired cerebellar activity underlying autism-like behavior in mice.
Autorzy :
Suliman-Lavie R; Department of Genetics, The Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel.
Title B; Department of Neurobiology, The Institute of Life Sciences and Edmond & Lily Safra Center for Brain Sciences (ELSC), The Hebrew University of Jerusalem, Jerusalem, Israel.
Cohen Y; Department of Genetics, The Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel.
Hamada N; Department of Molecular Neurobiology, Institute for Developmental Research, Aichi Developmental Disability Center, Kasugai, Japan.
Tal M; Department of Genetics, The Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel.
Tal N; Department of Genetics, The Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel.
Monderer-Rothkoff G; Department of Genetics, The Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel.
Gudmundsdottir B; Cellular and Molecular Therapeutics Branch, National Heart Lung and Blood Institutes (NHLBI)/National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK), National Institutes of Health (NIH), Bethesda, MD, USA.
Gudmundsson KO; Mouse Cancer Genetics Program, Center for Cancer Research, National Cancer Institute at Frederick, Bldg. 560/12-70, 1050 Boyles Street, Frederick, MD, 21702, USA.; Basic Research Program, Leidos Biomedical Research Inc, Frederick National Laboratory for Cancer Research, Bldg. 560/32-31D, 1050 Boyles Street, Frederick, MD, 21702, USA.
Keller JR; Mouse Cancer Genetics Program, Center for Cancer Research, National Cancer Institute at Frederick, Bldg. 560/12-70, 1050 Boyles Street, Frederick, MD, 21702, USA.; Basic Research Program, Leidos Biomedical Research Inc, Frederick National Laboratory for Cancer Research, Bldg. 560/32-31D, 1050 Boyles Street, Frederick, MD, 21702, USA.
Huang GJ; Department and Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, Taiwan.
Nagata KI; Department of Molecular Neurobiology, Institute for Developmental Research, Aichi Developmental Disability Center, Kasugai, Japan.; Department of Neurochemistry, Nagoya University Graduate School of Medicine, Nagoya, Japan.
Yarom Y; Department of Neurobiology, The Institute of Life Sciences and Edmond & Lily Safra Center for Brain Sciences (ELSC), The Hebrew University of Jerusalem, Jerusalem, Israel. .
Shifman S; Department of Genetics, The Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel. .
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Źródło :
Nature communications [Nat Commun] 2020 Nov 17; Vol. 11 (1), pp. 5836. Date of Electronic Publication: 2020 Nov 17.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
MeSH Terms :
Behavior, Animal*
Autism Spectrum Disorder/*etiology
Brain/*physiopathology
DNA Transposable Elements/*genetics
Purkinje Cells/*physiology
Transposases/*metabolism
Animals ; Autism Spectrum Disorder/genetics ; Brain/anatomy & histology ; Brain/embryology ; Brain/growth & development ; Cognition Disorders/genetics ; Disease Models, Animal ; Female ; Gene Expression Regulation ; HEK293 Cells ; Humans ; Learning ; Male ; Mice, Inbred ICR ; Mice, Knockout ; Microcephaly/genetics ; Motor Activity/genetics ; Neurogenesis/genetics ; Pregnancy ; Purkinje Cells/pathology ; Social Behavior ; Transcription, Genetic ; Transposases/deficiency
Czasopismo naukowe
Tytuł :
Nuclei Isolation from Fresh Frozen Brain Tumors for Single-Nucleus RNA-seq and ATAC-seq.
Autorzy :
Narayanan A; Neurology Clinic and National Center for Tumor Diseases, University Hospital Heidelberg.
Blanco-Carmona E; Bioinformatics and Omics Data Analytics, German Cancer Research Center (DKFZ).
Demirdizen E; Neurology Clinic and National Center for Tumor Diseases, University Hospital Heidelberg.
Sun X; Neurology Clinic and National Center for Tumor Diseases, University Hospital Heidelberg; Clinical Cooperation Unit Neurooncology, German Cancer Research Center (DKFZ).
Herold-Mende C; Division of Experimental Neurosurgery, Department of Neurosurgery, University of Heidelberg, INF 400, Heidelberg, Germany.
Schlesner M; Bioinformatics and Omics Data Analytics, German Cancer Research Center (DKFZ).
Turcan S; Neurology Clinic and National Center for Tumor Diseases, University Hospital Heidelberg; .
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Źródło :
Journal of visualized experiments : JoVE [J Vis Exp] 2020 Aug 25 (162). Date of Electronic Publication: 2020 Aug 25.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't; Video-Audio Media
MeSH Terms :
Freezing*
High-Throughput Nucleotide Sequencing*
RNA-Seq*
Brain Neoplasms/*pathology
Cell Nucleus/*metabolism
Chromatin/*metabolism
Transposases/*metabolism
Centrifugation ; Dissection ; Flow Cytometry ; Glioma/pathology ; Humans ; RNA, Small Nuclear/genetics ; RNA, Small Nuclear/metabolism ; Transposases/genetics
Czasopismo naukowe
Tytuł :
Structural basis of seamless excision and specific targeting by piggyBac transposase.
Autorzy :
Chen Q; Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, 20892, USA.
Luo W; Department of Medicine, Division and Nephrology and Hypertension, Vanderbilt University Medical Center, Nashville, TN, USA.; Department of Veterans Affairs, Nashville, TN, USA.
Veach RA; Department of Medicine, Division and Nephrology and Hypertension, Vanderbilt University Medical Center, Nashville, TN, USA.; Department of Veterans Affairs, Nashville, TN, USA.
Hickman AB; Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, 20892, USA.
Wilson MH; Department of Medicine, Division and Nephrology and Hypertension, Vanderbilt University Medical Center, Nashville, TN, USA.; Department of Veterans Affairs, Nashville, TN, USA.; Department of Pharmacology, Vanderbilt University, Nashville, TN, USA.
Dyda F; Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, 20892, USA. .
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Źródło :
Nature communications [Nat Commun] 2020 Jul 10; Vol. 11 (1), pp. 3446. Date of Electronic Publication: 2020 Jul 10.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural; Research Support, N.I.H., Intramural; Research Support, U.S. Gov't, Non-P.H.S.
MeSH Terms :
DNA Transposable Elements/*genetics
Transposases/*metabolism
Cryoelectron Microscopy ; Humans ; Magnetic Resonance Spectroscopy ; Protein Binding ; Protein Structure, Secondary ; Transposases/genetics
Czasopismo naukowe
Tytuł :
Comment on "RNA-guided DNA insertion with CRISPR-associated transposases".
Autorzy :
Rice PA; Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, IL, USA. .
Craig NL; Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Dyda F; Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD, USA.
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Źródło :
Science (New York, N.Y.) [Science] 2020 Jun 05; Vol. 368 (6495).
Typ publikacji :
Journal Article; Comment
MeSH Terms :
Clustered Regularly Interspaced Short Palindromic Repeats*
Transposases*
CRISPR-Cas Systems ; DNA Transposable Elements ; RNA
Czasopismo naukowe
Tytuł :
Response to Comment on "RNA-guided DNA insertion with CRISPR-associated transposases".
Autorzy :
Strecker J; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.; McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.; Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Ladha A; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.; McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.; Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Makarova KS; National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA.
Koonin EV; National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA.
Zhang F; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA. .; McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.; Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
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Źródło :
Science (New York, N.Y.) [Science] 2020 Jun 05; Vol. 368 (6495).
Typ publikacji :
Journal Article; Comment
MeSH Terms :
Clustered Regularly Interspaced Short Palindromic Repeats*
Transposases*
DNA Transposable Elements ; Plasmids ; RNA
Czasopismo naukowe
Tytuł :
The domesticated transposase ALP2 mediates formation of a novel Polycomb protein complex by direct interaction with MSI1, a core subunit of Polycomb Repressive Complex 2 (PRC2).
Autorzy :
Velanis CN; Institute of Molecular Plant Science, School of Biological Sciences, University of Edinburgh, Daniel Rutherford Building, Max Born Crescent, Edinburgh, United Kingdom.
Perera P; Institute of Molecular Plant Science, School of Biological Sciences, University of Edinburgh, Daniel Rutherford Building, Max Born Crescent, Edinburgh, United Kingdom.
Thomson B; Smurfit Institute of Genetics, Trinity College Dublin, Ireland.
de Leau E; Institute of Molecular Plant Science, School of Biological Sciences, University of Edinburgh, Daniel Rutherford Building, Max Born Crescent, Edinburgh, United Kingdom.
Liang SC; Institute of Molecular Plant Science, School of Biological Sciences, University of Edinburgh, Daniel Rutherford Building, Max Born Crescent, Edinburgh, United Kingdom.
Hartwig B; Department of Plant Developmental Biology, Max Planck Institute for Plant Breeding Research, Köln, Germany.
Förderer A; Department of Plant Developmental Biology, Max Planck Institute for Plant Breeding Research, Köln, Germany.
Thornton H; Institute of Molecular Plant Science, School of Biological Sciences, University of Edinburgh, Daniel Rutherford Building, Max Born Crescent, Edinburgh, United Kingdom.
Arede P; Institute of Molecular Plant Science, School of Biological Sciences, University of Edinburgh, Daniel Rutherford Building, Max Born Crescent, Edinburgh, United Kingdom.
Chen J; Institute of Molecular Plant Science, School of Biological Sciences, University of Edinburgh, Daniel Rutherford Building, Max Born Crescent, Edinburgh, United Kingdom.
Webb KM; Wellcome Centre for Cell Biology, School of Biological Sciences, University of Edinburgh, Max Born Crescent, Edinburgh, United Kingdom.
Gümüs S; Department of Biotechnology, Mannheim University of Applied Science, Mannheim, Germany.
De Jaeger G; Department of Plant Biotechnology and Bioinformatics, Ghent University, Gent, Belgium.; VIB Center for Plant Systems Biology, Gent, Belgium.
Page CA; Department of Biology & Geology, University of South Carolina Aiken, Aiken, South Carolina, United States of America.
Hancock CN; Department of Biology & Geology, University of South Carolina Aiken, Aiken, South Carolina, United States of America.
Spanos C; Wellcome Centre for Cell Biology, School of Biological Sciences, University of Edinburgh, Max Born Crescent, Edinburgh, United Kingdom.
Rappsilber J; Wellcome Centre for Cell Biology, School of Biological Sciences, University of Edinburgh, Max Born Crescent, Edinburgh, United Kingdom.; Bioanalytics Unit, Institute of Biotechnology, Technische Universität Berlin, Berlin, Germany.
Voigt P; Wellcome Centre for Cell Biology, School of Biological Sciences, University of Edinburgh, Max Born Crescent, Edinburgh, United Kingdom.
Turck F; Department of Plant Developmental Biology, Max Planck Institute for Plant Breeding Research, Köln, Germany.
Wellmer F; Smurfit Institute of Genetics, Trinity College Dublin, Ireland.
Goodrich J; Institute of Molecular Plant Science, School of Biological Sciences, University of Edinburgh, Daniel Rutherford Building, Max Born Crescent, Edinburgh, United Kingdom.
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Źródło :
PLoS genetics [PLoS Genet] 2020 May 28; Vol. 16 (5), pp. e1008681. Date of Electronic Publication: 2020 May 28 (Print Publication: 2020).
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Arabidopsis*/enzymology
Arabidopsis*/genetics
Arabidopsis*/metabolism
Arabidopsis Proteins/*metabolism
Polycomb-Group Proteins/*metabolism
Repressor Proteins/*metabolism
Transposases/*physiology
Animals ; Arabidopsis Proteins/chemistry ; Arabidopsis Proteins/genetics ; Catalytic Domain/genetics ; Cells, Cultured ; Domestication ; Gene Expression Regulation, Plant ; Plants, Genetically Modified ; Polycomb-Group Proteins/genetics ; Protein Binding ; Protein Subunits/chemistry ; Protein Subunits/genetics ; Protein Subunits/metabolism ; Repressor Proteins/chemistry ; Repressor Proteins/genetics ; Sf9 Cells ; Spodoptera ; Transposases/genetics
Czasopismo naukowe
Tytuł :
Transposon-mediated generation of CAR-T cells shows efficient anti B-cell leukemia response after ex vivo expansion.
Autorzy :
Chicaybam L; Molecular Carcinogenesis Program - Research Coordination - Brazilian National Cancer Institute (INCA), Rio de Janeiro, Brazil.; Vice-Presidency of Research and Biological Collections (VPPCB) - Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro, Brazil.
Abdo L; Molecular Carcinogenesis Program - Research Coordination - Brazilian National Cancer Institute (INCA), Rio de Janeiro, Brazil.
Viegas M; Molecular Carcinogenesis Program - Research Coordination - Brazilian National Cancer Institute (INCA), Rio de Janeiro, Brazil.
Marques LVC; Molecular Carcinogenesis Program - Research Coordination - Brazilian National Cancer Institute (INCA), Rio de Janeiro, Brazil.
de Sousa P; Molecular Carcinogenesis Program - Research Coordination - Brazilian National Cancer Institute (INCA), Rio de Janeiro, Brazil.
Batista-Silva LR; Molecular Carcinogenesis Program - Research Coordination - Brazilian National Cancer Institute (INCA), Rio de Janeiro, Brazil.
Alves-Monteiro V; Molecular Carcinogenesis Program - Research Coordination - Brazilian National Cancer Institute (INCA), Rio de Janeiro, Brazil.
Bonecker S; Bone Marrow Transplantation Unit, Specialized Laboratories, Laboratory of Molecular Biology, National Cancer Institute (INCA), Rio de Janeiro, Brazil.
Monte-Mór B; Bone Marrow Transplantation Unit, Specialized Laboratories, Laboratory of Molecular Biology, National Cancer Institute (INCA), Rio de Janeiro, Brazil.
Bonamino MH; Molecular Carcinogenesis Program - Research Coordination - Brazilian National Cancer Institute (INCA), Rio de Janeiro, Brazil. .; Vice-Presidency of Research and Biological Collections (VPPCB) - Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro, Brazil. .
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Źródło :
Gene therapy [Gene Ther] 2020 Feb; Vol. 27 (1-2), pp. 85-95. Date of Electronic Publication: 2020 Jan 09.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Immunotherapy, Adoptive/*methods
Leukemia, B-Cell/*therapy
Transposases/*therapeutic use
Animals ; Antigens, CD19/genetics ; Cell Line, Tumor ; Cytotoxicity, Immunologic/genetics ; Cytotoxicity, Immunologic/immunology ; Female ; Genetic Vectors/genetics ; Genetic Vectors/therapeutic use ; Humans ; Mice ; Mice, Inbred NOD ; Mice, SCID ; Precursor Cell Lymphoblastic Leukemia-Lymphoma/therapy ; Receptors, Antigen, T-Cell/genetics ; T-Lymphocytes/immunology ; Transposases/genetics ; Xenograft Model Antitumor Assays
Czasopismo naukowe
Tytuł :
Structures of a RAG-like transposase during cut-and-paste transposition.
Autorzy :
Liu C; Department of Immunobiology, Yale School of Medicine, New Haven, CT, USA.
Yang Y; Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT, USA.; Howard Hughes Medical Institute, Yale University, New Haven, CT, USA.
Schatz DG; Department of Immunobiology, Yale School of Medicine, New Haven, CT, USA. .
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Źródło :
Nature [Nature] 2019 Nov; Vol. 575 (7783), pp. 540-544. Date of Electronic Publication: 2019 Nov 13.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
MeSH Terms :
Biocatalysis*
Homeodomain Proteins*/chemistry
Homeodomain Proteins*/metabolism
Homeodomain Proteins*/ultrastructure
Moths/*enzymology
Transposases/*chemistry
Transposases/*metabolism
Amino Acid Sequence ; Animals ; Apoenzymes/chemistry ; Apoenzymes/metabolism ; Apoenzymes/ultrastructure ; Base Sequence ; Cryoelectron Microscopy ; Crystallography, X-Ray ; DNA/chemistry ; DNA/genetics ; DNA/metabolism ; DNA Cleavage ; DNA-Binding Proteins ; Evolution, Molecular ; Models, Molecular ; Moths/ultrastructure ; Protein Domains ; Transposases/ultrastructure
Czasopismo naukowe
Tytuł :
A single amino acid switch converts the Sleeping Beauty transposase into an efficient unidirectional excisionase with utility in stem cell reprogramming.
Autorzy :
Kesselring L; Transposition and Genome Engineering, Division of Medical Biotechnology, Paul Ehrlich Institute, Langen, Germany.
Miskey C; Transposition and Genome Engineering, Division of Medical Biotechnology, Paul Ehrlich Institute, Langen, Germany.
Zuliani C; Structural and Computational Biology Unit, European Molecular Biology Laboratory, Heidelberg 69117, Germany.
Querques I; Structural and Computational Biology Unit, European Molecular Biology Laboratory, Heidelberg 69117, Germany.
Kapitonov V; Transposition and Genome Engineering, Division of Medical Biotechnology, Paul Ehrlich Institute, Langen, Germany.
Laukó A; BioTalentum Ltd, Gödöllő, 2100 Gödöllő, Hungary.
Fehér A; BioTalentum Ltd, Gödöllő, 2100 Gödöllő, Hungary.
Palazzo A; Department of Biology, University of Bari 'Aldo Moro', Italy.
Diem T; Transposition and Genome Engineering, Division of Medical Biotechnology, Paul Ehrlich Institute, Langen, Germany.
Lustig J; Transposition and Genome Engineering, Division of Medical Biotechnology, Paul Ehrlich Institute, Langen, Germany.
Sebe A; Transposition and Genome Engineering, Division of Medical Biotechnology, Paul Ehrlich Institute, Langen, Germany.
Wang Y; Mobile DNA, Max Delbrück Center for Molecular Medicine, Berlin, Germany.
Dinnyés A; BioTalentum Ltd, Gödöllő, 2100 Gödöllő, Hungary.
Izsvák Z; Mobile DNA, Max Delbrück Center for Molecular Medicine, Berlin, Germany.
Barabas O; Structural and Computational Biology Unit, European Molecular Biology Laboratory, Heidelberg 69117, Germany.
Ivics Z; Transposition and Genome Engineering, Division of Medical Biotechnology, Paul Ehrlich Institute, Langen, Germany.
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Źródło :
Nucleic acids research [Nucleic Acids Res] 2020 Jan 10; Vol. 48 (1), pp. 316-331.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Cellular Reprogramming*
DNA Transposable Elements*
Induced Pluripotent Stem Cells/*metabolism
Transposases/*genetics
Amino Acid Substitution ; Animals ; Epistasis, Genetic ; Genetic Engineering/methods ; HeLa Cells ; Hep G2 Cells ; Humans ; Induced Pluripotent Stem Cells/cytology ; Mice ; Mutation ; Transposases/metabolism
Czasopismo naukowe
Tytuł :
First Biochemical Steps on Bacterial Transposition Pathways.
Autorzy :
Guynet C; Laboratoire de Microbiologie et Génétique Moléculaires, Centre de Biologie Intégrative (CBI), Centre National de la Recherche Scientifique (CNRS), Université de Toulouse, UPS, Toulouse, France. .
Nicolas E; Laboratory of Evolutionary Genetics and Ecology (LEGE), Department of Biology-URBE, University of Namur (UNamur), Namur, Belgium.
Ton-Hoang B; Laboratoire de Microbiologie et Génétique Moléculaires, Centre de Biologie Intégrative (CBI), Centre National de la Recherche Scientifique (CNRS), Université de Toulouse, UPS, Toulouse, France.
Bouet JY; Laboratoire de Microbiologie et Génétique Moléculaires, Centre de Biologie Intégrative (CBI), Centre National de la Recherche Scientifique (CNRS), Université de Toulouse, UPS, Toulouse, France.
Hallet B; Louvain Institute of Biomolecular Science and Technology (LIBST), Université Catholique de Louvain (UCLouvain), Louvain-la-Neuve, Belgium. .
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Źródło :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2020; Vol. 2075, pp. 157-177.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
DNA Transposable Elements*
Bacteria/*genetics
Bacteria/*metabolism
Transposases/*metabolism
Bacterial Physiological Phenomena ; DNA-Binding Proteins/metabolism ; Electrophoretic Mobility Shift Assay ; Gene Expression ; Macromolecular Substances/metabolism ; Protein Binding ; Staining and Labeling ; Temperature ; Transposases/genetics
Czasopismo naukowe
Tytuł :
Engineering rules that minimize germline silencing of transgenes in simple extrachromosomal arrays in C. elegans.
Autorzy :
Aljohani MD; King Abdullah University of Science and Technology (KAUST), Biological and Environmental Science and Engineering Division (BESE), KAUST Environmental Epigenetics Program (KEEP), Thuwal, 23955-6900, Saudi Arabia.
El Mouridi S; King Abdullah University of Science and Technology (KAUST), Biological and Environmental Science and Engineering Division (BESE), KAUST Environmental Epigenetics Program (KEEP), Thuwal, 23955-6900, Saudi Arabia.
Priyadarshini M; King Abdullah University of Science and Technology (KAUST), Biological and Environmental Science and Engineering Division (BESE), KAUST Environmental Epigenetics Program (KEEP), Thuwal, 23955-6900, Saudi Arabia.
Vargas-Velazquez AM; King Abdullah University of Science and Technology (KAUST), Biological and Environmental Science and Engineering Division (BESE), KAUST Environmental Epigenetics Program (KEEP), Thuwal, 23955-6900, Saudi Arabia.
Frøkjær-Jensen C; King Abdullah University of Science and Technology (KAUST), Biological and Environmental Science and Engineering Division (BESE), KAUST Environmental Epigenetics Program (KEEP), Thuwal, 23955-6900, Saudi Arabia. .
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Źródło :
Nature communications [Nat Commun] 2020 Dec 09; Vol. 11 (1), pp. 6300. Date of Electronic Publication: 2020 Dec 09.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
MeSH Terms :
Gene Silencing*
Genetic Engineering/*methods
Introns/*genetics
Animals ; Animals, Genetically Modified/genetics ; CRISPR-Associated Protein 9/genetics ; Caenorhabditis elegans/genetics ; Codon/genetics ; DNA-Binding Proteins/genetics ; Genes, Reporter/genetics ; Genetic Vectors/genetics ; Germ Cells ; Luminescent Proteins/genetics ; Transgenes/genetics ; Transposases/genetics
Czasopismo naukowe
Tytuł :
An Engineered Cas-Transposon System for Programmable and Site-Directed DNA Transpositions.
Autorzy :
Chen SP; Department of Systems Biology, Columbia University Medical Center, New York, New York.; Integrated Program in Cellular, Molecular and Biomedical Studies, Columbia University Medical Center, New York, New York.
Wang HH; Department of Systems Biology, Columbia University Medical Center, New York, New York.; Department of Pathology and Cell Biology, Columbia University Medical Center, New York, New York.
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Źródło :
The CRISPR journal [CRISPR J] 2019 Dec; Vol. 2 (6), pp. 376-394. Date of Electronic Publication: 2019 Nov 19.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
MeSH Terms :
DNA Transposable Elements/*genetics
Mutagenesis, Insertional/*methods
Transposases/*metabolism
CRISPR-Associated Protein 9/genetics ; CRISPR-Associated Protein 9/metabolism ; CRISPR-Cas Systems/genetics ; CRISPR-Cas Systems/physiology ; DNA Transposable Elements/physiology ; Endonucleases/genetics ; Escherichia coli/genetics ; Gene Editing/methods ; Recombination, Genetic/genetics ; Recombination, Genetic/physiology ; Transposases/genetics
Czasopismo naukowe
Tytuł :
Jump ahead with a twist: DNA acrobatics drive transposition forward.
Autorzy :
Arinkin V; Structural and Computational Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany.
Smyshlyaev G; Structural and Computational Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany; European Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI), Wellcome Genome Campus, Hinxton, UK.
Barabas O; Structural and Computational Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany. Electronic address: .
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Źródło :
Current opinion in structural biology [Curr Opin Struct Biol] 2019 Dec; Vol. 59, pp. 168-177. Date of Electronic Publication: 2019 Oct 05.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Review
MeSH Terms :
DNA Transposable Elements*
Transposases/*metabolism
Animals ; DNA Breaks, Single-Stranded ; DNA Cleavage ; Humans ; Recombination, Genetic ; Structure-Activity Relationship ; Transposases/chemistry
Czasopismo naukowe
Tytuł :
In vivo CRISPR screening in CD8 T cells with AAV-Sleeping Beauty hybrid vectors identifies membrane targets for improving immunotherapy for glioblastoma.
Autorzy :
Ye L; System Biology Institute, Integrated Science & Technology Center, West Haven, CT, USA.; Department of Genetics, Yale University School of Medicine, New Haven, CT, USA.; Center for Cancer Systems Biology, Integrated Science & Technology Center, West Haven, CT, USA.
Park JJ; System Biology Institute, Integrated Science & Technology Center, West Haven, CT, USA.; Department of Genetics, Yale University School of Medicine, New Haven, CT, USA.; Center for Cancer Systems Biology, Integrated Science & Technology Center, West Haven, CT, USA.; Yale M.D.-Ph.D. Program, Yale University School of Medicine, New Haven, CT, USA.
Dong MB; System Biology Institute, Integrated Science & Technology Center, West Haven, CT, USA.; Department of Genetics, Yale University School of Medicine, New Haven, CT, USA.; Center for Cancer Systems Biology, Integrated Science & Technology Center, West Haven, CT, USA.; Yale M.D.-Ph.D. Program, Yale University School of Medicine, New Haven, CT, USA.; Immunobiology Program, Yale University School of Medicine, New Haven, CT, USA.; Department of Immunobiology, Yale University School of Medicine, New Haven, CT, USA.
Yang Q; System Biology Institute, Integrated Science & Technology Center, West Haven, CT, USA.; Department of Genetics, Yale University School of Medicine, New Haven, CT, USA.; Center for Cancer Systems Biology, Integrated Science & Technology Center, West Haven, CT, USA.
Chow RD; System Biology Institute, Integrated Science & Technology Center, West Haven, CT, USA.; Department of Genetics, Yale University School of Medicine, New Haven, CT, USA.; Center for Cancer Systems Biology, Integrated Science & Technology Center, West Haven, CT, USA.; Yale M.D.-Ph.D. Program, Yale University School of Medicine, New Haven, CT, USA.
Peng L; System Biology Institute, Integrated Science & Technology Center, West Haven, CT, USA.; Department of Genetics, Yale University School of Medicine, New Haven, CT, USA.; Center for Cancer Systems Biology, Integrated Science & Technology Center, West Haven, CT, USA.
Du Y; System Biology Institute, Integrated Science & Technology Center, West Haven, CT, USA.; Department of Genetics, Yale University School of Medicine, New Haven, CT, USA.; Center for Cancer Systems Biology, Integrated Science & Technology Center, West Haven, CT, USA.
Guo J; System Biology Institute, Integrated Science & Technology Center, West Haven, CT, USA.; Department of Genetics, Yale University School of Medicine, New Haven, CT, USA.; Center for Cancer Systems Biology, Integrated Science & Technology Center, West Haven, CT, USA.
Dai X; System Biology Institute, Integrated Science & Technology Center, West Haven, CT, USA.; Department of Genetics, Yale University School of Medicine, New Haven, CT, USA.; Center for Cancer Systems Biology, Integrated Science & Technology Center, West Haven, CT, USA.
Wang G; System Biology Institute, Integrated Science & Technology Center, West Haven, CT, USA.; Department of Genetics, Yale University School of Medicine, New Haven, CT, USA.; Center for Cancer Systems Biology, Integrated Science & Technology Center, West Haven, CT, USA.
Errami Y; System Biology Institute, Integrated Science & Technology Center, West Haven, CT, USA.; Department of Genetics, Yale University School of Medicine, New Haven, CT, USA.; Center for Cancer Systems Biology, Integrated Science & Technology Center, West Haven, CT, USA.
Chen S; System Biology Institute, Integrated Science & Technology Center, West Haven, CT, USA. .; Department of Genetics, Yale University School of Medicine, New Haven, CT, USA. .; Center for Cancer Systems Biology, Integrated Science & Technology Center, West Haven, CT, USA. .; Yale M.D.-Ph.D. Program, Yale University School of Medicine, New Haven, CT, USA. .; Immunobiology Program, Yale University School of Medicine, New Haven, CT, USA. .; Department of Neurosurgery, Yale University School of Medicine, New Haven, CT, USA. .; Yale Comprehensive Cancer Center, Yale University School of Medicine, New Haven, CT, USA. .; Yale Stem Cell Center, Yale University School of Medicine, New Haven, CT, USA. .; Yale Liver Center, Yale University School of Medicine, New Haven, CT, USA. .; Yale Center for Biomedical Data Science, Yale University School of Medicine, New Haven, CT, USA. .
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Źródło :
Nature biotechnology [Nat Biotechnol] 2019 Nov; Vol. 37 (11), pp. 1302-1313. Date of Electronic Publication: 2019 Sep 23.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
MeSH Terms :
CD8-Positive T-Lymphocytes/*transplantation
Gene Editing/*methods
Glioblastoma/*therapy
Membrane Proteins/*genetics
Transposases/*genetics
Animals ; Antigens, CD/genetics ; CD8-Positive T-Lymphocytes/metabolism ; CRISPR-Cas Systems ; Cell Line, Tumor ; Dependovirus/genetics ; Female ; Glioblastoma/genetics ; Glioblastoma/immunology ; Humans ; Immunotherapy, Adoptive ; Male ; Mice ; N-Acetylglucosaminyltransferases/genetics ; Neoplasm Proteins/genetics ; Protein Disulfide-Isomerases/genetics ; RNA, Guide/genetics ; Receptors, Cell Surface/genetics ; Transposases/metabolism ; Xenograft Model Antitumor Assays
Czasopismo naukowe
Tytuł :
An analysis of the IS 6 /IS 26 family of insertion sequences: is it a single family?
Autorzy :
Harmer CJ; School of Life and Environmental Sciences, University of Sydney, NSW 2006, Australia.
Hall RM; School of Life and Environmental Sciences, University of Sydney, NSW 2006, Australia.
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Źródło :
Microbial genomics [Microb Genom] 2019 Sep; Vol. 5 (9). Date of Electronic Publication: 2019 Sep 05.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Archaeal Proteins/*metabolism
Bacterial Proteins/*metabolism
DNA Transposable Elements/*genetics
Transposases/*metabolism
Amino Acid Sequence ; Archaea/genetics ; Archaeal Proteins/chemistry ; Archaeal Proteins/genetics ; Bacterial Proteins/chemistry ; Bacterial Proteins/genetics ; Catalytic Domain ; Databases, Genetic ; Gram-Negative Bacteria/genetics ; Helix-Turn-Helix Motifs/genetics ; Mycobacterium fortuitum/genetics ; Sequence Alignment ; Terminal Repeat Sequences/genetics ; Transposases/chemistry ; Transposases/genetics
Czasopismo naukowe
Tytuł :
Efficient low-cost chromatin profiling with CUT&Tag.
Autorzy :
Kaya-Okur HS; Basic Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA.; Howard Hughes Medical Institute, Chevy Chase, MD, USA.; Altius Institute for Biomedical Sciences, Seattle, WA, USA.
Janssens DH; Basic Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA.
Henikoff JG; Basic Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA.
Ahmad K; Basic Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA.
Henikoff S; Basic Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA. .; Howard Hughes Medical Institute, Chevy Chase, MD, USA. .
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Źródło :
Nature protocols [Nat Protoc] 2020 Oct; Vol. 15 (10), pp. 3264-3283. Date of Electronic Publication: 2020 Sep 10.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
MeSH Terms :
Chromatin/*genetics
Chromosome Mapping/*methods
Epigenomics/*methods
Base Sequence ; DNA/genetics ; Gene Library ; High-Throughput Nucleotide Sequencing/methods ; Histones/metabolism ; Sequence Analysis, DNA/methods ; Single-Cell Analysis/methods ; Transposases/genetics ; Transposases/metabolism
Czasopismo naukowe
Tytuł :
Single-cell lineage tracing approaches in hematology research: technical considerations.
Autorzy :
Carrelha J; Haematopoietic Stem Cell Laboratory, MRC Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, UK; MRC Molecular Haematology Unit, MRC Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, UK.
Lin DS; Immunology, The Walter & Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia; Faculty of Medicine, Dentistry & Health Sciences, University of Melbourne, Parkville, Victoria, Australia.
Rodriguez-Fraticelli AE; Stem Cell Program, Boston Children's Hospital, Boston, Massachusetts, USA; Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, Massachusetts, USA.
Luis TC; Department of Life Sciences, Imperial College London, London, UK.
Wilkinson AC; Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, CA, USA; Department of Genetics, Stanford University School of Medicine, Stanford, CA, USA.
Cabezas-Wallscheid N; Max Planck Institute of Immunobiology and Epigenetics, Freiburg, Germany.
Tremblay CS; Australian Centre for Blood Diseases, Central Clinical School, Monash University, Melbourne, Australia.
Haas S; Heidelberg Institute for Stem Cell Technology and Experimental Medicine (HI-STEM gGmbH), Heidelberg, Germany; Division of Stem Cells and Cancer, Deutsches Krebsforschungszentrum (DKFZ) and DKFZ-ZMBH Alliance, Heidelberg, Germany. Electronic address: .
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Źródło :
Experimental hematology [Exp Hematol] 2020 Sep; Vol. 89, pp. 26-36. Date of Electronic Publication: 2020 Jul 28.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
MeSH Terms :
Cell Tracking/*methods
Cell Transplantation/*methods
Hematology/*methods
Hematopoiesis/*genetics
Hematopoietic Stem Cells/*cytology
Animals ; Cell Differentiation ; Cell Lineage/genetics ; Cell Lineage/immunology ; Congresses as Topic ; DNA Barcoding, Taxonomic/methods ; Genetic Vectors/chemistry ; Genetic Vectors/metabolism ; Hematopoiesis/immunology ; Hematopoietic Stem Cells/immunology ; Hematopoietic Stem Cells/virology ; Homeostasis/genetics ; Homeostasis/immunology ; Humans ; Lentivirus/genetics ; Lentivirus/metabolism ; Mice ; Single-Cell Analysis/methods ; Transgenes ; Transposases/genetics ; Transposases/immunology
Czasopismo naukowe
Tytuł :
Transposition: A CRISPR Way to Get Around.
Autorzy :
Dimitriu T; Environment and Sustainability Institute, Biosciences, University of Exeter, Cornwall Campus, Penryn, Cornwall TR10 9FE, UK.
Ashby B; Department of Mathematical Sciences, University of Bath, Claverton Down, Bath, Somerset BA2 7AY, UK.
Westra ER; Environment and Sustainability Institute, Biosciences, University of Exeter, Cornwall Campus, Penryn, Cornwall TR10 9FE, UK. Electronic address: .
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Źródło :
Current biology : CB [Curr Biol] 2019 Sep 23; Vol. 29 (18), pp. R886-R889.
Typ publikacji :
Journal Article; Comment
MeSH Terms :
Clustered Regularly Interspaced Short Palindromic Repeats*
Transposases*
CRISPR-Cas Systems ; DNA Transposable Elements ; RNA
Czasopismo naukowe
Tytuł :
Targeted DNA transposition in vitro using a dCas9-transposase fusion protein.
Autorzy :
Bhatt S; School of Life Sciences, University of Nottingham, Queen's Medical Centre, Nottingham NG7 2UH, UK.
Chalmers R; School of Life Sciences, University of Nottingham, Queen's Medical Centre, Nottingham NG7 2UH, UK.
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Źródło :
Nucleic acids research [Nucleic Acids Res] 2019 Sep 05; Vol. 47 (15), pp. 8126-8135.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
CRISPR-Associated Protein 9/*genetics
DNA Transposable Elements/*genetics
Recombinant Fusion Proteins/*genetics
Transposases/*genetics
Base Sequence ; Binding Sites/genetics ; CRISPR-Associated Protein 9/metabolism ; DNA, Bacterial/genetics ; DNA, Bacterial/metabolism ; Escherichia coli/genetics ; Escherichia coli/metabolism ; Kinetics ; Mutagenesis, Insertional ; Recombinant Fusion Proteins/metabolism ; Transgenes/genetics ; Transposases/metabolism
Czasopismo naukowe

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