- Tytuł:
- Overexpression of the Nocardia lactamdurans alpha-aminoadipyl-cysteinyl-valine synthetase in Streptomyces lividans. The purified multienzyme uses cystathionine and 6-oxopiperidine 2-carboxylate as substrates for synthesis of the tripeptide.
- Autorzy:
- Źródło:
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European journal of biochemistry [EurJ Biochem] 1996 Dec 01; Vol. 242 (2), pp. 264-70. - Typ publikacji:
- Journal Article; Research Support, Non-U.S. Gov't
- MeSH Terms:
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Cystathionine/*metabolism
Nocardia/*enzymology
Peptide Synthases/*metabolism
Pipecolic Acids/*metabolism
Streptomyces/*metabolism
Chromatography, Gel ; Chromatography, High Pressure Liquid ; Chromatography, Ion Exchange ; Cloning, Molecular/methods ; Kinetics ; Multienzyme Complexes/biosynthesis ; Multienzyme Complexes/isolation & purification ; Multienzyme Complexes/metabolism ; Nocardia/genetics ; Peptide Synthases/biosynthesis ; Peptide Synthases/isolation & purification ; Recombinant Proteins/biosynthesis ; Recombinant Proteins/isolation & purification ; Recombinant Proteins/metabolism ; Restriction Mapping ; Substrate Specificity ; Ultrafiltration
Czasopismo naukowe