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Tytuł :
Glycosylation Profiling of Glycoproteins Secreted from Cultured Cells Using Glycan Node Analysis and GC-MS.
Autorzy :
Aguilar Díaz de León JS; School of Molecular Sciences, The Biodesign Institute-Center for Personalized Diagnostics, Arizona State University, Tempe, AZ, USA.
Borges CR; School of Molecular Sciences, The Biodesign Institute-Center for Personalized Diagnostics, Arizona State University, Tempe, AZ, USA. .
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Źródło :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2271, pp. 317-330.
Typ publikacji :
Journal Article
MeSH Terms :
Gas Chromatography-Mass Spectrometry*
Glycomics*
Protein Processing, Post-Translational*
Glycoproteins/*analysis
Hepatocytes/*metabolism
Immunoglobulin G/*analysis
Polysaccharides/*analysis
Culture Media, Conditioned/metabolism ; Glycosylation ; Hep G2 Cells ; Hepatocytes/drug effects ; Humans ; Interleukin-1beta/pharmacology ; Interleukin-6/pharmacology ; Methylation ; Neuraminidase/metabolism ; Research Design ; Secretory Pathway ; Workflow
Czasopismo naukowe
Tytuł :
Determination of Isomeric Glycan Structures by Permethylation and Liquid Chromatography-Mass Spectrometry (LC-MS).
Autorzy :
Cho BG; Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX, USA.
Banazadeh A; Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX, USA.
Peng W; Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX, USA.
Zhao J; Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX, USA.
Goli M; Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX, USA.
Gautam S; Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX, USA.
Hussein A; Department of Biotechnology, Institute of Graduate Studies and Research, University of Alexandria, Alexandria, Egypt.
Mechref Y; Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX, USA. .
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Źródło :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2271, pp. 281-301.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural
MeSH Terms :
Chromatography, Liquid*
Glycomics*
Protein Processing, Post-Translational*
Spectrometry, Mass, Electrospray Ionization*
Tandem Mass Spectrometry*
Glycoproteins/*analysis
Polysaccharides/*analysis
Carbohydrate Conformation ; Glycoside Hydrolases/metabolism ; Glycosylation ; Graphite/chemistry ; Isomerism ; Methylation ; Porosity ; Research Design ; Workflow
Czasopismo naukowe
Tytuł :
High-temperature LC-MS/MS of permethylated glycans derived from glycoproteins.
Autorzy :
Zhou S; Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX, USA.
Hu Y; Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX, USA.
Mechref Y; Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX, USA.
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Źródło :
Electrophoresis [Electrophoresis] 2016 Jun; Vol. 37 (11), pp. 1506-13. Date of Electronic Publication: 2016 Apr 09.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
MeSH Terms :
Glycomics/*methods
Glycoproteins/*chemistry
Polysaccharides/*analysis
Animals ; Chromatography, Liquid ; Humans ; Isomerism ; Sensitivity and Specificity ; Tandem Mass Spectrometry ; Temperature
Czasopismo naukowe
Tytuł :
Protein and site specificity of fucosylation in liver-secreted glycoproteins.
Autorzy :
Pompach P; Institute of Microbiology v.v.i., Czech Academy of Sciences , Videnska 1083, Prague 142 20, Czech Republic.
Ashline DJ
Brnakova Z
Benicky J
Sanda M
Goldman R
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Źródło :
Journal of proteome research [J Proteome Res] 2014 Dec 05; Vol. 13 (12), pp. 5561-9. Date of Electronic Publication: 2014 Oct 10.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
MeSH Terms :
Protein Processing, Post-Translational*
Glycoproteins/*blood
Liver/*metabolism
Amino Acid Sequence ; Carbohydrate Conformation ; Carbohydrate Sequence ; Glycoproteins/metabolism ; Glycosylation ; Hepatitis C/blood ; Humans ; Liver Cirrhosis/blood ; Liver Cirrhosis/virology ; Male ; Middle Aged ; Molecular Sequence Data
Czasopismo naukowe

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