Temat: "protein refolding" - OpacWWW

Skocz do pozycji: 1.

Tytuł:: High level SARS-CoV-2 nucleocapsid refolding using mild condition for inclusion bodies solubilization: Application of high pressure at pH 9.0.
Autorzy:: Chura-Chambi RM; Centro de Biotecnologia, Instituto de Pesquisas Energéticas e Nucleares, IPEN-CNEN/SP, São Paulo, SP, Brazil.
Prieto-da-Silva ARB; Laboratório de Genética, Instituto Butantan, São Paulo, SP, Brazil.
Di Lela MM; Centro de Biotecnologia, Instituto de Pesquisas Energéticas e Nucleares, IPEN-CNEN/SP, São Paulo, SP, Brazil.
Oliveira JE; Centro de Biotecnologia, Instituto de Pesquisas Energéticas e Nucleares, IPEN-CNEN/SP, São Paulo, SP, Brazil.
Abreu PEA; Laboratório de Bacteriologia, Instituto Butantan, São Paulo, SP, Brazil.
Meireles LR; Laboratório de Protozoologia, Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, São Paulo,SP, Brazil.
de Andrade Junior HF; Laboratório de Protozoologia, Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, São Paulo,SP, Brazil.
Morganti L; Centro de Biotecnologia, Instituto de Pesquisas Energéticas e Nucleares, IPEN-CNEN/SP, São Paulo, SP, Brazil.; Pokaż więcej
Źródło:: PloS one [PLoS One] 2022 Feb 03; Vol. 17 (2), pp. e0262591. Date of Electronic Publication: 2022 Feb 03 (Print Publication: 2022).
Typ publikacji:: Journal Article
MeSH Terms:: Protein Refolding*
Antibodies, Viral/*blood
Antigens, Viral/*chemistry
COVID-19/*blood
COVID-19/*diagnosis
Coronavirus Nucleocapsid Proteins/*chemistry
Immunoglobulin G/*blood
Inclusion Bodies/*chemistry
SARS-CoV-2/*immunology
Antibodies, Viral/immunology ; Antigens, Viral/immunology ; COVID-19/virology ; Coronavirus Nucleocapsid Proteins/immunology ; Enzyme-Linked Immunosorbent Assay/methods ; Escherichia coli/genetics ; Escherichia coli/metabolism ; Humans ; Hydrogen-Ion Concentration ; Hydrostatic Pressure ; Immunoglobulin G/immunology ; Phosphoproteins/chemistry ; Phosphoproteins/immunology ; Protein Structure, Tertiary ; Recombinant Proteins/chemistry ; Recombinant Proteins/immunology ; Solubility

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Skocz do pozycji: 2.

Tytuł:: Human growth hormone inclusion bodies present native-like secondary and tertiary structures which can be preserved by mild solubilization for refolding.
Autorzy:: Chura-Chambi RM; Centro de Biotecnologia, Instituto de Pesquisas Energéticas e Nucleares, IPEN-CNEN/SP, São Paulo, SP, Brazil.
Farah CS; Departamento de Bioquímica, Instituto de Química da Universidade de São Paulo, São Paulo, SP, Brazil.
Morganti L; Centro de Biotecnologia, Instituto de Pesquisas Energéticas e Nucleares, IPEN-CNEN/SP, São Paulo, SP, Brazil. .; Pokaż więcej
Źródło:: Microbial cell factories [Microb Cell Fact] 2022 Aug 17; Vol. 21 (1), pp. 164. Date of Electronic Publication: 2022 Aug 17.
Typ publikacji:: Journal Article
MeSH Terms:: Human Growth Hormone*/metabolism
Inclusion Bodies*/metabolism
Humans ; Escherichia coli/genetics ; Escherichia coli/metabolism ; Protein Refolding ; Protein Structure, Secondary ; Protein Structure, Tertiary ; Recombinant Proteins/biosynthesis ; Solubility

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Skocz do pozycji: 3.

Tytuł:: Refolding and characterization of two G protein-coupled receptors purified from E. coli inclusion bodies.
Autorzy:: Heim B; Institute of Applied Biotechnology, University of Applied Sciences, Biberach, Germany.
Handrick R; Institute of Applied Biotechnology, University of Applied Sciences, Biberach, Germany.
Hartmann MD; Max Planck Institute for Developmental Biology, Tübingen, Germany.
Kiefer H; Institute of Applied Biotechnology, University of Applied Sciences, Biberach, Germany.; Pokaż więcej
Źródło:: PloS one [PLoS One] 2021 Feb 24; Vol. 16 (2), pp. e0247689. Date of Electronic Publication: 2021 Feb 24 (Print Publication: 2021).
Typ publikacji:: Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:: Protein Refolding*
Escherichia coli/*metabolism
Escherichia coli Proteins/*metabolism
Inclusion Bodies/*metabolism
Receptors, G-Protein-Coupled/*metabolism
Sphingosine-1-Phosphate Receptors/*metabolism

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Skocz do pozycji: 4.

Tytuł:: Advances in monitoring and control of refolding kinetics combining PAT and modeling.
Autorzy:: Pauk JN; Research Area Biochemical Engineering, Institute of Chemical, Environmental and Bioscience Engineering, Vienna University of Technology, Gumpendorferstrasse 1a/166, 1060, Vienna, Austria.; Competence Center CHASE GmbH, Altenbergerstraße 69, 4040, Linz, Austria.
Raju Palanisamy J; Research Area Biochemical Engineering, Institute of Chemical, Environmental and Bioscience Engineering, Vienna University of Technology, Gumpendorferstrasse 1a/166, 1060, Vienna, Austria.
Kager J; Research Area Biochemical Engineering, Institute of Chemical, Environmental and Bioscience Engineering, Vienna University of Technology, Gumpendorferstrasse 1a/166, 1060, Vienna, Austria.
Koczka K; Bilfinger Industrietechnik Salzburg GmbH, Mooslackengasse 17, 1190, Vienna, Austria.
Berghammer G; Bilfinger Industrietechnik Salzburg GmbH, Mooslackengasse 17, 1190, Vienna, Austria.
Herwig C; Research Area Biochemical Engineering, Institute of Chemical, Environmental and Bioscience Engineering, Vienna University of Technology, Gumpendorferstrasse 1a/166, 1060, Vienna, Austria. .
Veiter L; Research Area Biochemical Engineering, Institute of Chemical, Environmental and Bioscience Engineering, Vienna University of Technology, Gumpendorferstrasse 1a/166, 1060, Vienna, Austria.; Competence Center CHASE GmbH, Altenbergerstraße 69, 4040, Linz, Austria.; Pokaż więcej
Źródło:: Applied microbiology and biotechnology [Appl Microbiol Biotechnol] 2021 Mar; Vol. 105 (6), pp. 2243-2260. Date of Electronic Publication: 2021 Feb 17.
Typ publikacji:: Journal Article; Review
MeSH Terms:: Inclusion Bodies*
Protein Folding*
Kinetics ; Protein Refolding ; Recombinant Proteins/genetics ; Technology

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Skocz do pozycji: 5.

Tytuł:: Association of high pressure and alkaline condition for solubilization of inclusion bodies and refolding of the NS1 protein from zika virus.
Autorzy:: Rosa da Silva CM; Instituto de Pesquisas Energéticas e Nucleares, IPEN-CNEN/SP, Centro de Biotecnologia, Av. Prof. Lineu Prestes, 2242, São Paulo, 05508-000, Brazil.
Chura-Chambi RM; Instituto de Pesquisas Energéticas e Nucleares, IPEN-CNEN/SP, Centro de Biotecnologia, Av. Prof. Lineu Prestes, 2242, São Paulo, 05508-000, Brazil.
Ramos Pereira L; Departamento de Microbiologia, Universidade de São Paulo, Instituto de Ciências Biomédicas, Av. Prof. Lineu Prestes ,1374, São Paulo, 05508-000, Brazil.
Cordeiro Y; Universidade Federal do Rio de Janeiro, Faculdade de Farmácia, Av. Carlos Chagas Filho 373, Rio de Janeiro, 21941-902, Brazil.
de Souza Ferreira LC; Departamento de Microbiologia, Universidade de São Paulo, Instituto de Ciências Biomédicas, Av. Prof. Lineu Prestes ,1374, São Paulo, 05508-000, Brazil.
Morganti L; Instituto de Pesquisas Energéticas e Nucleares, IPEN-CNEN/SP, Centro de Biotecnologia, Av. Prof. Lineu Prestes, 2242, São Paulo, 05508-000, Brazil. .; Pokaż więcej
Źródło:: BMC biotechnology [BMC Biotechnol] 2018 Dec 12; Vol. 18 (1), pp. 78. Date of Electronic Publication: 2018 Dec 12.
Typ publikacji:: Evaluation Study; Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:: Biochemistry/*methods
Inclusion Bodies/*chemistry
Viral Nonstructural Proteins/*chemistry
Zika Virus/*metabolism
Alkalies/chemistry ; Hydrostatic Pressure ; Inclusion Bodies/genetics ; Inclusion Bodies/metabolism ; Protein Refolding ; Protein Structure, Secondary ; Solubility ; Viral Nonstructural Proteins/genetics ; Viral Nonstructural Proteins/metabolism ; Zika Virus/chemistry ; Zika Virus/genetics

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Skocz do pozycji: 6.

Tytuł:: Protein recovery from inclusion bodies of Escherichia coli using mild solubilization process.
Autorzy:: Singh A; Product Development Cell, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi, 110067, India. .
Upadhyay V; Product Development Cell, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi, 110067, India. .
Upadhyay AK; Product Development Cell, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi, 110067, India. .
Singh SM; Product Development Cell, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi, 110067, India. .
Panda AK; Product Development Cell, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi, 110067, India. .; Pokaż więcej
Źródło:: Microbial cell factories [Microb Cell Fact] 2015 Mar 25; Vol. 14, pp. 41. Date of Electronic Publication: 2015 Mar 25.
Typ publikacji:: Journal Article; Research Support, Non-U.S. Gov't; Review
MeSH Terms:: Protein Refolding*
Escherichia coli/*metabolism
Inclusion Bodies/*metabolism
Recombinant Proteins/*chemistry
Recombinant Proteins/*metabolism
Escherichia coli/genetics ; Models, Molecular ; Protein Denaturation ; Protein Folding ; Protein Unfolding ; Solubility

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Skocz do pozycji: 7.

Tytuł:: Disulfide linkages in Plasmodium falciparum plasmepsin-i are essential elements for its processing activity and multi-milligram recombinant production yield.
Autorzy:: Lolupiman S; Department of Biochemistry and Center for Excellence in Protein Structure and Function, Faculty of Science, Mahidol University, Bangkok, Thailand.
Siripurkpong P; Department of Medical Technology, Faculty of Allied Health Sciences, Thammasat University, Pathumthani, Thailand.
Yuvaniyama J; Department of Biochemistry and Center for Excellence in Protein Structure and Function, Faculty of Science, Mahidol University, Bangkok, Thailand.; Pokaż więcej
Źródło:: PloS one [PLoS One] 2014 Feb 20; Vol. 9 (2), pp. e89424. Date of Electronic Publication: 2014 Feb 20 (Print Publication: 2014).
Typ publikacji:: Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:: Protein Refolding*
Aspartic Acid Endopeptidases/*isolation & purification
Aspartic Acid Endopeptidases/*metabolism
Disulfides/*chemistry
Inclusion Bodies/*metabolism
Plasmodium falciparum/*metabolism
Recombinant Proteins/*isolation & purification
Amino Acid Sequence ; Aspartic Acid Endopeptidases/genetics ; Disulfides/metabolism ; Humans ; Molecular Sequence Data ; Plasmodium falciparum/genetics ; Protein Engineering ; Recombinant Proteins/genetics ; Recombinant Proteins/metabolism ; Substrate Specificity

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Skocz do pozycji: 8.

Tytuł:: Refolding techniques for recovering biologically active recombinant proteins from inclusion bodies.
Autorzy:: Yamaguchi H; Liberal Arts Education Center, Aso Campus, Tokai University, Minamiaso, Kumamoto 869-1404, Japan. .
Miyazaki M; Measurement Solution Research Center, National Institute of Advanced Industrial Science and Technology, Tosu, Saga 841-0052, Japan. .; Pokaż więcej
Źródło:: Biomolecules [Biomolecules] 2014 Feb 20; Vol. 4 (1), pp. 235-51. Date of Electronic Publication: 2014 Feb 20.
Typ publikacji:: Evaluation Study; Journal Article; Research Support, Non-U.S. Gov't; Review
MeSH Terms:: Protein Refolding*
Biochemistry/*methods
Inclusion Bodies/*metabolism
Recombinant Proteins/*chemistry
Recombinant Proteins/*metabolism
Escherichia coli/genetics ; Escherichia coli/metabolism ; Inclusion Bodies/chemistry ; Inclusion Bodies/genetics ; Recombinant Proteins/genetics

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Skocz do pozycji: 9.

Tytuł:: Recovery of bioactive protein from bacterial inclusion bodies using trifluoroethanol as solubilization agent.
Autorzy:: Upadhyay V; Product Development Cell, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi, 110 067, India.
Singh A; Product Development Cell, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi, 110 067, India.
Jha D; Product Development Cell, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi, 110 067, India.
Singh A; Product Development Cell, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi, 110 067, India.
Panda AK; Product Development Cell, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi, 110 067, India. .; Pokaż więcej
Źródło:: Microbial cell factories [Microb Cell Fact] 2016 Jun 08; Vol. 15, pp. 100. Date of Electronic Publication: 2016 Jun 08.
Typ publikacji:: Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:: Escherichia coli/*metabolism
Inclusion Bodies/*metabolism
Recombinant Proteins/*biosynthesis
Trifluoroethanol/*chemistry
Chromatography, Gel ; Chromatography, High Pressure Liquid ; Circular Dichroism ; Electrophoresis, Polyacrylamide Gel ; Human Growth Hormone/genetics ; Human Growth Hormone/metabolism ; Humans ; Inclusion Bodies/chemistry ; Protein Refolding ; Protein Structure, Secondary ; Protein Structure, Tertiary ; Recombinant Proteins/chemistry ; Recombinant Proteins/isolation & purification

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Skocz do pozycji: 10.

Tytuł:: A Rapid Method for Refolding Cell Surface Receptors and Ligands.
Autorzy:: Zhai L; Department of Biochemistry, Virginia Polytechnic Institute &State University, Blacksburg, VA 24061, USA.; Interdepartmental Microbiology Graduate Program, Virginia Polytechnic Institute &State University, Blacksburg, VA 24061, USA.
Wu L; Department of Biochemistry, Virginia Polytechnic Institute &State University, Blacksburg, VA 24061, USA.
Li F; Department of Biochemistry, Virginia Polytechnic Institute &State University, Blacksburg, VA 24061, USA.
Burnham RS; Department of Biochemistry, Virginia Polytechnic Institute &State University, Blacksburg, VA 24061, USA.
Pizarro JC; Department of Tropical Medicine, Tulane University, New Orleans, LA 70112, USA.
Xu B; Department of Biochemistry, Virginia Polytechnic Institute &State University, Blacksburg, VA 24061, USA.; Interdepartmental Microbiology Graduate Program, Virginia Polytechnic Institute &State University, Blacksburg, VA 24061, USA.; Center for Drug Discovery, Virginia Polytechnic Institute &State University, Blacksburg, VA 24061, USA.; Pokaż więcej
Źródło:: Scientific reports [Sci Rep] 2016 May 24; Vol. 6, pp. 26482. Date of Electronic Publication: 2016 May 24.
Typ publikacji:: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
MeSH Terms:: Inclusion Bodies/*metabolism
Receptors, Cell Surface/*chemistry
Circular Dichroism ; Inclusion Bodies/chemistry ; Ligands ; Models, Molecular ; Protein Refolding ; Receptors, Cell Surface/isolation & purification ; Recombinant Proteins/chemistry

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Skocz do pozycji: 11.

Tytuł:: Refolding of proteins from inclusion bodies: rational design and recipes.
Autorzy:: Basu A; School of Chemical and Biomedical Engineering, Nanyang Technological University, 62 Nanyang Drive, Singapore, Singapore.
Li X
Leong SS; Pokaż więcej
Źródło:: Applied microbiology and biotechnology [Appl Microbiol Biotechnol] 2011 Oct; Vol. 92 (2), pp. 241-51. Date of Electronic Publication: 2011 Aug 07.
Typ publikacji:: Evaluation Study; Journal Article; Review
MeSH Terms:: Protein Refolding*
Inclusion Bodies/*chemistry
Industrial Microbiology/*methods
Recombinant Proteins/*chemistry
Animals ; Bacteria/genetics ; Bacteria/metabolism ; Humans ; Inclusion Bodies/genetics ; Inclusion Bodies/metabolism ; Industrial Microbiology/instrumentation ; Kinetics ; Recombinant Proteins/genetics ; Recombinant Proteins/metabolism

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Skocz do pozycji: 12.

Tytuł:: Characterization of the 4,6-α-glucanotransferase GTFB enzyme of Lactobacillus reuteri 121 isolated from inclusion bodies.
Autorzy:: Bai Y; Microbial Physiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Nijenborgh 7, 9747 AG, Groningen, The Netherlands. .; The State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, 214122, China. .
van der Kaaij RM; Microbial Physiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Nijenborgh 7, 9747 AG, Groningen, The Netherlands. .
Woortman AJ; Department of Polymer Chemistry, Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, The Netherlands. .
Jin Z; The State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, 214122, China. .
Dijkhuizen L; Microbial Physiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Nijenborgh 7, 9747 AG, Groningen, The Netherlands. .; Pokaż więcej
Źródło:: BMC biotechnology [BMC Biotechnol] 2015 Jun 09; Vol. 15, pp. 49. Date of Electronic Publication: 2015 Jun 09.
Typ publikacji:: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:: Escherichia coli/*genetics
Glycogen Debranching Enzyme System/*biosynthesis
Glycogen Debranching Enzyme System/*chemistry
Inclusion Bodies/*enzymology
Limosilactobacillus reuteri/*genetics
Bacterial Proteins/biosynthesis ; Bacterial Proteins/chemistry ; Bacterial Proteins/isolation & purification ; Enzyme Stability ; Escherichia coli/metabolism ; Glycogen Debranching Enzyme System/isolation & purification ; Inclusion Bodies/chemistry ; Limosilactobacillus reuteri/enzymology ; Models, Molecular ; Protein Denaturation ; Protein Refolding ; Protein Structure, Secondary ; Solubility ; Substrate Specificity