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Wyświetlanie 1-20 z 35644
Tytuł:
Evaluating Two Steps in Transcription Using a Fluorescence-Based Electrophoretic Mobility Shift Assay
Autorzy:
Singh, Anoushka
Miller, Ryan C.
Archuleta, Stephen R.
Kugel, Jennifer F. (ORCID 0000-0003-1086-290X)
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Deskryptory:
Molecular Biology
Science Instruction
Genetics
Teaching Methods
Laboratory Experiments
Biochemistry
Scientific Research
Scientific Principles
Źródło:
Biochemistry and Molecular Biology Education. Mar-Apr 2023 51(2):230-235.
Recenzowane naukowo:
Y
Page Count:
6
Sponsoring Agency:
National Science Foundation (NSF)
Czasopismo naukowe
Szczegóły
Tytuł:
A possible role of gas-phase electrophoretic mobility molecular analysis (nES GEMMA) in extracellular vesicle research.
Autorzy:
Steinberger S; Institute of Chemical Technologies and Analytics, TU Wien, Getreidemarkt 9/164 CTA, A-1060, Vienna, Austria.
Karuthedom George S; Center for Biomedical Technology, Department for Biomedical Research, Danube University Krems, Krems, Austria.
Lauková L; Center for Biomedical Technology, Department for Biomedical Research, Danube University Krems, Krems, Austria.
Weiss R; Center for Biomedical Technology, Department for Biomedical Research, Danube University Krems, Krems, Austria.
Tripisciano C; Center for Biomedical Technology, Department for Biomedical Research, Danube University Krems, Krems, Austria.
Birner-Gruenberger R; Institute of Chemical Technologies and Analytics, TU Wien, Getreidemarkt 9/164 CTA, A-1060, Vienna, Austria.
Weber V; Center for Biomedical Technology, Department for Biomedical Research, Danube University Krems, Krems, Austria.
Allmaier G; Institute of Chemical Technologies and Analytics, TU Wien, Getreidemarkt 9/164 CTA, A-1060, Vienna, Austria.
Weiss VU; Institute of Chemical Technologies and Analytics, TU Wien, Getreidemarkt 9/164 CTA, A-1060, Vienna, Austria. .
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Źródło:
Analytical and bioanalytical chemistry [Anal Bioanal Chem] 2021 Dec; Vol. 413 (30), pp. 7341-7352. Date of Electronic Publication: 2021 Oct 07.
Typ publikacji:
Journal Article
MeSH Terms:
Electrophoretic Mobility Shift Assay/*methods
Extracellular Vesicles/*metabolism
Spectrometry, Mass, Electrospray Ionization/*methods
Gases ; Humans ; Spectrometry, Mass, Electrospray Ionization/instrumentation
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknieSzczegóły
Tytuł:
From an electrophoretic mobility shift assay to isolated transcription factors: a fast genomic-proteomic approach.
Autorzy:
Mach-Aigner AR; Department of Gene Technology and Applied Biochemistry, Institute of Chemical Engineering, TU Wien, Austria. />Grosstessner-Hain K
Poças-Fonseca MJ
Mechtler K
Mach RL
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Źródło:
BMC genomics [BMC Genomics] 2010 Nov 18; Vol. 11, pp. 644. Date of Electronic Publication: 2010 Nov 18.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
Electrophoretic Mobility Shift Assay*
Fungal Proteins/*isolation & purification
Hypocrea/*metabolism
Proteomics/*methods
Transcription Factors/*isolation & purification
Base Sequence ; Carbon/pharmacology ; Chromatography, Affinity ; Cloning, Molecular ; Fungal Proteins/chemistry ; Fungal Proteins/genetics ; Glucose/pharmacology ; Hypocrea/drug effects ; Hypocrea/genetics ; Hypocrea/growth & development ; Molecular Sequence Data ; Promoter Regions, Genetic/genetics ; Protein Binding/drug effects ; Protein Biosynthesis/drug effects ; Repressor Proteins/genetics ; Repressor Proteins/metabolism ; Streptavidin/metabolism ; Transcription Factors/genetics ; Transcription Factors/metabolism ; Transcription, Genetic/drug effects
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknieSzczegóły
Tytuł:
Determination of equilibrium dissociation constants for recombinant antibodies by high-throughput affinity electrophoresis.
Autorzy:
Pan Y; University of California, Berkeley - UCSF Graduate Program in Bioengineering, Berkeley, CA, 94720, USA.
Sackmann EK; Labcyte Inc., 1190 Borregas Ave, Sunnyvale, CA, 94089, USA.
Wypisniak K; Department of Pharmaceutical Chemistry, University of California, San Francisco, CA, 94158 USA.
Hornsby M; Department of Pharmaceutical Chemistry, University of California, San Francisco, CA, 94158 USA.
Datwani SS; Labcyte Inc., 1190 Borregas Ave, Sunnyvale, CA, 94089, USA.
Herr AE; University of California, Berkeley - UCSF Graduate Program in Bioengineering, Berkeley, CA, 94720, USA.; Department of Bioengineering, University of California, Berkeley, CA, 94720, USA.
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Źródło:
Scientific reports [Sci Rep] 2016 Dec 23; Vol. 6, pp. 39774. Date of Electronic Publication: 2016 Dec 23.
Typ publikacji:
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
MeSH Terms:
Antibody Affinity*
Electrophoresis, Polyacrylamide Gel*
Electrophoretic Mobility Shift Assay*
Immunoglobulin Fab Fragments/*chemistry
Animals ; Humans ; Recombinant Proteins/chemistry
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknieSzczegóły
Tytuł:
Electromobility Shift Assay Reveals Evidence in Favor of Allele-Specific Binding of RUNX1 to the 5' Hypersensitive Site 4-Locus Control Region.
Autorzy:
Dehghani H; a Genetics Research Center, University of Social Welfare and Rehabilitation Sciences , Tehran , Iran.
Ghobakhloo S; a Genetics Research Center, University of Social Welfare and Rehabilitation Sciences , Tehran , Iran.
Neishabury M; a Genetics Research Center, University of Social Welfare and Rehabilitation Sciences , Tehran , Iran.
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Źródło:
Hemoglobin [Hemoglobin] 2016 Aug; Vol. 40 (4), pp. 236-9.
Typ publikacji:
Journal Article
MeSH Terms:
Alleles*
Electrophoretic Mobility Shift Assay*
Locus Control Region*
Core Binding Factor Alpha 2 Subunit/*metabolism
Binding Sites ; Humans ; Inverted Repeat Sequences ; Phenotype ; Protein Binding
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknie Pełny tekstSzczegóły
Tytuł:
Protocol to detect nucleotide-protein interaction in vitro using a non-radioactive competitive electrophoretic mobility shift assay
Autorzy:
Fang Wang
Ting Yao
Wen Yang
Pan Wu
Yutao Liu
Bin Yang
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Temat:
Microbiology
Molecular biology
Molecular/Chemical probes
Protein biochemistry
Protein expression and purification
Science (General)
Q1-390
Źródło:
STAR Protocols, Vol 3, Iss 4, Pp 101730- (2022)
Opis pliku:
electronic resource
Relacje:
http://www.sciencedirect.com/science/article/pii/S2666166722006104; https://doaj.org/toc/2666-1667
Dostęp URL:
https://doaj.org/article/7b36a8ca63ee4e7c89f04eaa42c78736  Link otwiera się w nowym oknie
Czasopismo naukowe
Szczegóły
Tytuł:
Rapid preparation of high-purity nuclear proteins from a small number of cultured cells for use in electrophoretic mobility shift assays.
Autorzy:
Luo Y; Laboratory of Molecular Diagnostics, Department of Mycobacteriology, Leprosy Research Center, National Institute of Infectious Diseases, 4-2-1 Aoba-cho, Higashimurayama-shi, 189-0002, Tokyo, Japan. .
Hara T; Laboratory of Molecular Diagnostics, Department of Mycobacteriology, Leprosy Research Center, National Institute of Infectious Diseases, 4-2-1 Aoba-cho, Higashimurayama-shi, 189-0002, Tokyo, Japan. .
Ishido Y; Laboratory of Molecular Diagnostics, Department of Mycobacteriology, Leprosy Research Center, National Institute of Infectious Diseases, 4-2-1 Aoba-cho, Higashimurayama-shi, 189-0002, Tokyo, Japan. .
Yoshihara A; Laboratory of Molecular Diagnostics, Department of Mycobacteriology, Leprosy Research Center, National Institute of Infectious Diseases, 4-2-1 Aoba-cho, Higashimurayama-shi, 189-0002, Tokyo, Japan. .
Oda K; Laboratory of Molecular Diagnostics, Department of Mycobacteriology, Leprosy Research Center, National Institute of Infectious Diseases, 4-2-1 Aoba-cho, Higashimurayama-shi, 189-0002, Tokyo, Japan. .
Makino M; Department of Mycobacteriology, Leprosy Research Center, National Institute of Infectious Diseases, 4-2-1 Aoba-cho, Higashimurayama-shi, 189-0002, Tokyo, Japan. .
Ishii N; Leprosy Research Center, National Institute of Infectious Diseases, 4-2-1 Aoba-cho, Higashimurayama-shi, 189-0002, Tokyo, Japan. .
Hiroi N; Department of Education Planning and Development, Faculty of Medicine, Toho University, Tokyo, 143-8540, Japan. .
Suzuki K; Laboratory of Molecular Diagnostics, Department of Mycobacteriology, Leprosy Research Center, National Institute of Infectious Diseases, 4-2-1 Aoba-cho, Higashimurayama-shi, 189-0002, Tokyo, Japan. .
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Źródło:
BMC immunology [BMC Immunol] 2014 Dec 20; Vol. 15, pp. 586. Date of Electronic Publication: 2014 Dec 20.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
Electrophoretic Mobility Shift Assay*
NF-kappa B/*chemistry
NF-kappa B/*isolation & purification
Cell Line, Tumor ; Humans
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknieSzczegóły
Tytuł:
Validation of miRNA-mRNA interactions by electrophoretic mobility shift assays.
Autorzy:
Solé A
Mencia N
Villalobos X
Noé V
Ciudad CJ; Department of Biochemistry and Molecular Biology, School of Pharmacy, University of Barcelona, Avenue Diagonal 643, Barcelona E-08028, Spain. .
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Źródło:
BMC research notes [BMC Res Notes] 2013 Nov 12; Vol. 6, pp. 454. Date of Electronic Publication: 2013 Nov 12.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't; Validation Study
MeSH Terms:
Electrophoretic Mobility Shift Assay*
3' Untranslated Regions/*genetics
MicroRNAs/*chemistry
Ribonucleotides/*chemistry
Sodium-Bicarbonate Symporters/*chemistry
Binding Sites ; Gene Expression Regulation ; Humans ; MicroRNAs/genetics ; Phosphorus Radioisotopes ; RNA Stability ; Ribonucleotides/genetics ; Sodium-Bicarbonate Symporters/genetics
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknieSzczegóły
Tytuł:
Analysis of enzyme-treated red blood cell surface and haemagglutination using a theory of soft-particle electrophoresis.
Autorzy:
Hyono A; Faculty of Pharmaceutical Sciences, Tokyo University of Sciences, Yamazaki, Noda, Chiba, Japan. />Mazda T
Okazaki H
Tadokoro K
Ohshima H
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Źródło:
Vox sanguinis [Vox Sang] 2008 Aug; Vol. 95 (2), pp. 131-6. Date of Electronic Publication: 2008 May 28.
Typ publikacji:
Journal Article
MeSH Terms:
Electrophoretic Mobility Shift Assay*
Hemagglutination Tests*
Models, Biological*
Erythrocyte Membrane/*drug effects
Hemagglutination/*drug effects
Neuraminidase/*pharmacology
Papain/*pharmacology
Agglutinins/immunology ; Antigen-Antibody Reactions/drug effects ; Arthrobacter/enzymology ; Bacterial Proteins/pharmacology ; Cell Size/drug effects ; Colloids ; Duffy Blood-Group System/immunology ; Erythrocyte Membrane/immunology ; Hemagglutination/physiology ; Humans ; Isoantibodies/immunology ; Membrane Potentials/drug effects ; Receptors, Cell Surface/immunology ; Rh-Hr Blood-Group System/immunology ; Rho(D) Immune Globulin ; Surface Properties/drug effects
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknieSzczegóły
Tytuł:
A microfluidics-based mobility shift assay to identify new inhibitors of β-secretase for Alzheimer's disease.
Autorzy:
Liu R; State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, China.; Shanghai ChemPartner Co. Ltd., 998 Halei Road, Shanghai, 201203, China.
Liu YC; Shanghai ChemPartner Co. Ltd., 998 Halei Road, Shanghai, 201203, China.
Meng J; Shanghai ChemPartner Co. Ltd., 998 Halei Road, Shanghai, 201203, China.
Zhu H; Shanghai ChemPartner Co. Ltd., 998 Halei Road, Shanghai, 201203, China.
Zhang X; State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, China. .
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Źródło:
Analytical and bioanalytical chemistry [Anal Bioanal Chem] 2017 Nov; Vol. 409 (28), pp. 6635-6642. Date of Electronic Publication: 2017 Sep 09.
Typ publikacji:
Journal Article; Validation Study
MeSH Terms:
Amyloid Precursor Protein Secretases/*antagonists & inhibitors
Aspartic Acid Endopeptidases/*antagonists & inhibitors
Drug Evaluation, Preclinical/*methods
Electrophoretic Mobility Shift Assay/*methods
Microfluidic Analytical Techniques/*methods
Alzheimer Disease/drug therapy ; Alzheimer Disease/enzymology ; Amyloid Precursor Protein Secretases/metabolism ; Aspartic Acid Endopeptidases/metabolism ; Enzyme Assays/methods ; Humans ; Kinetics ; Recombinant Proteins/metabolism
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknieSzczegóły
Tytuł:
Assessing 2D electrophoretic mobility spectroscopy (2D MOSY) for analytical applications.
Autorzy:
Fang Y; Division of Applied Physical Chemistry, Department of Chemistry, KTH Royal Institute of Technology, Teknikringen 30, SE-10044, Stockholm, Sweden.
Yushmanov PV; P&L Scientific Instrument Services, Box 1241, SE-18124, Lidingö, Sweden.
Furó I; Division of Applied Physical Chemistry, Department of Chemistry, KTH Royal Institute of Technology, Teknikringen 30, SE-10044, Stockholm, Sweden.
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Źródło:
Magnetic resonance in chemistry : MRC [Magn Reson Chem] 2017 Jun; Vol. 55 (6), pp. 584-588. Date of Electronic Publication: 2017 Feb 01.
Typ publikacji:
Journal Article
MeSH Terms:
Complex Mixtures/*analysis
Electrophoretic Mobility Shift Assay/*methods
Magnetic Resonance Spectroscopy/*methods
Acetaminophen/analysis ; Amino Acids/analysis ; Aspirin/analysis ; Caffeine/analysis ; Diffusion ; Hydrogen-Ion Concentration ; Tablets
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknieSzczegóły
Tytuł:
Surface coating and matrix effect on the electrophoretic mobility of gold nanoparticles: a capillary electrophoresis-inductively coupled plasma mass spectrometry study.
Autorzy:
Qu H; Office of Regulatory Affairs, Arkansas Regional Laboratory, U.S. Food and Drug Administration, 3900 NCTR Road, Jefferson, AR, 72079, USA.
Linder SW; Office of Regulatory Affairs, Arkansas Regional Laboratory, U.S. Food and Drug Administration, 3900 NCTR Road, Jefferson, AR, 72079, USA.
Mudalige TK; Office of Regulatory Affairs, Arkansas Regional Laboratory, U.S. Food and Drug Administration, 3900 NCTR Road, Jefferson, AR, 72079, USA. .
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Źródło:
Analytical and bioanalytical chemistry [Anal Bioanal Chem] 2017 Feb; Vol. 409 (4), pp. 979-988. Date of Electronic Publication: 2016 Oct 25.
Typ publikacji:
Journal Article
MeSH Terms:
Electrophoresis, Capillary/*methods
Electrophoretic Mobility Shift Assay/*methods
Gold/*chemistry
Mass Spectrometry/*methods
Metal Nanoparticles/*chemistry
Surface Properties
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknieSzczegóły
Tytuł:
MIST, a Novel Approach to Reveal Hidden Substrate Specificity in Aminoacyl-tRNA Synthetases.
Autorzy:
Eriani G; Architecture et Réactivité de l'ARN, Université de Strasbourg, CNRS, Institut de Biologie Moléculaire et Cellulaire, 67084, Strasbourg, CEDEX, France.
Karam J; Laboratory of tRNA Biology, Department of Biology, College of Charleston, Charleston, South Carolina, United States of America.
Jacinto J; Laboratory of tRNA Biology, Department of Biology, College of Charleston, Charleston, South Carolina, United States of America.
Morris Richard E; Laboratory of tRNA Biology, Department of Biology, College of Charleston, Charleston, South Carolina, United States of America.
Geslain R; Laboratory of tRNA Biology, Department of Biology, College of Charleston, Charleston, South Carolina, United States of America.
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Źródło:
PloS one [PLoS One] 2015 Jun 11; Vol. 10 (6), pp. e0130042. Date of Electronic Publication: 2015 Jun 11 (Print Publication: 2015).
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
Protein Biosynthesis*
Amino Acids/*metabolism
Amino Acyl-tRNA Synthetases/*metabolism
Electrophoretic Mobility Shift Assay/*methods
Microarray Analysis/*methods
RNA, Transfer/*metabolism
Saccharomyces cerevisiae/*metabolism
Amino Acids/genetics ; Amino Acyl-tRNA Synthetases/genetics ; Aminoacylation ; RNA, Transfer/genetics ; Saccharomyces cerevisiae/genetics ; Substrate Specificity
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknieSzczegóły
Tytuł:
Determination of nuclear transcription factor activity using a modified electrophoretic mobility shift assay
Autorzy:
Yong Wang
Wenhua Huang
Daizhi Peng
Yuanbin Hu
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Temat:
Nuclear transcription factor
electrophoretic mobility shift assay
methodology
modification
Biotechnology
TP248.13-248.65
Źródło:
Brazilian Archives of Biology and Technology, Vol 51, Iss 4, Pp 667-672 (2008)
Opis pliku:
electronic resource
Relacje:
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000400025; https://doaj.org/toc/1516-8913; https://doaj.org/toc/1678-4324
Dostęp URL:
https://doaj.org/article/c518fb9c092545d696c2ee891380f7f9  Link otwiera się w nowym oknie
Czasopismo naukowe
Szczegóły
Wyświetlanie 1-20 z 35644

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